METHOD FOR DEGRADING CANNABINOID

Abstract

Disclosed is a method for degrading cannabinoid, wherein the method carries out a photocatalytic reaction on a liquid containing cannabinoid, the method specifically comprising: (1) a photocatalytic reaction; (2) filtering; and (3) recycling of a photocatalyst. After the liquid containing the cannabinoid is treated using the method, the cannabinoid content is obviously reduced and can reach ppm-level. The raw materials and reagents used in the method are cheap and easy to obtain, the operation and testing methods are simple, and the photocatalyst can be recycled, avoiding waste of resources, and facilitating industrialization.

Claims

1. A method of degrading cannabinoid by a photocatalytic reaction on a solution containing cannabinoid.

2. The method for degrading cannabinoid according to claim 1, wherein the cannabinoid comprises one or a combination or two or more of tetrahydrocannabinol THC, cannabinol CBD, cannabigerol CBG, cannabichromene CBC, cannabinol CBN, and propyl homologues Δ9-tetrahydrocannabivarin THCV and cannabidivarin CBDV.

3. The method for degrading cannabinoid according to claim 1, wherein the method comprises the following steps: (1) placing the liquid containing cannabinoid in a container equipped with illuminance equipment, adding a photocatalyst and stirring at room temperature; and (2) standing the liquid in step (1), and filtering to obtain a filtrate and a filter cake.

4. The method for degrading cannabinoid according to claim 3, wherein the illumination equipment in step (1) has an illuminance of 2000˜20000 Lx.

5. The method for degrading cannabinoid according to claim 4, wherein the illuminance is 4500˜12000 Lx, and preferably 7000˜12000 Lx.

6. The method for degrading cannabinoid according to claim 3, wherein a mass-volume ratio of an amount of the photocatalyst to the solution containing cannabinoid in step (1) is 5% to 50% in mg/mL.

7. The method for degrading cannabinoid according to claim 6, wherein a mass-volume ratio of an amount of the photocatalyst to the solution containing cannabinoid is 10% to 30% in mg/mL, and preferably, the mass-volume ratio of the amount of the photocatalyst to the solution containing cannabinoid is 10% to 20% in mg/mL.

8. The method for degrading cannabinoid according to claim 3, wherein the photocatalyst in step (1) is selected from one or a combination of two or more of bismuth vanadate, bismuth oxychloride, bismuth vanadate/titanium dioxide composite, bismuth vanadate/bismuth oxychloride composite, bismuth oxychloride/titanium dioxide composite in any proportion; and preferably, the photocatalyst is bismuth vanadate or bismuth vanadate/bismuth oxychloride composite.

9. The method for degrading cannabinoid according to claim 3, wherein before the step (1), the method also comprises: (a) concentrating the liquid containing cannabinoid to obtain a thick paste; and (b) adding a solvent to the thick paste, stirring and dissolving to obtain an enriched liquid of the liquid containing cannabinoid; and the filter cake obtained in the step (2) is reused as a photocatalyst.

10. The method for degrading cannabinoid according to claim 9, wherein the solvent in step (b) is an organic solvent, preferably selected from one or a combination of two or more of ethanol, methanol, ethyl acetate, acetone, chloroform, petroleum ether, n-hexane, and n-heptane in any proportion, and more preferably, the organic solvent is selected from one or a combination of two or more of ethanol, methanol, ethyl acetate, and acetone in any proportion.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0035] FIG. 1 is a high-performance liquid chromatogram of standards CBDV, CBG, CBD, THCV, and THC in Example 1 of the invention.

[0036] FIG. 2 is a high-performance liquid chromatogram of a waste liquid sample before degradation in Example 1 of the invention.

[0037] FIG. 3 is a high-performance liquid chromatogram of a filtrate after degradation in Example 1 of the invention.

DETAILED DESCRIPTION

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the field of the invention. The following examples are used to illustrate the invention but not to limit it. The following abbreviations and their corresponding substances appearing in the invention are:

[0039] CBDV cannabidivarin

[0040] CBD cannabidiol

[0041] CBG cannabigerol

[0042] THCV Δ9-tetrahydrocannabivarin

[0043] THC tetrahydrocannabinol

[0044] In combination with the examples of the invention, the technical scheme of the invention will be described clearly and completely, and it is obvious that the described examples are only part of the examples of the invention, not all of the examples. Based on the examples of the invention, all other examples obtained by those skilled in the field without creative work shall fall within the protection scope of the invention.

Example 1

[0045] 1. The Steps of the Method for Degrading Cannabinoid in the Present Example are as Follows:

[0046] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 60° C. to obtain a thick paste;

[0047] (2) the thick paste was added with 95% ethanol, stirred for dissolution and dissolving to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0048] (3) the enriched liquid in step (2) was placed in a closed treatment tank with illumination equipment, and 10% (mg/mL) of photocatalyst bismuth vanadate was added, and the resulting mixture was stirred at room temperature for 30 minutes with illuminance of 7000 Lx;

[0049] (4) the liquid after reaction in step (3) was left standing for 12 hours, and then filtered to obtain a filtrate and a filter cake;

[0050] (5) the filter cake obtained in step (4) was added into water, and stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 3 times. The finally collected filter cake was dried in a vacuum environment at 50° C. for 24 hours to obtain a recovered photocatalyst; and

[0051] (6) the reserved sample of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

[0052] 2. Methods of Sample Detection and Analysis

[0053] High-performance liquid chromatography detection conditions and system applicability test: octadecyl silane bonded silica gel was used as a filler, 0.1% formic acid aqueous solution as mobile phase A, 0.1% formic acid in acetonitrile as mobile phase B, the elution procedure is as follows, the detection wavelength was 220 nm, and the number of theoretical plates calculated by CBD was not less than 12000.

[0054] When the elution time was 0-6 minutes, the volume concentration of the mobile phase: mobile phase A: 30%, and mobile phase B: balance; when the elution time was 6-12 minutes, the volume concentration of the mobile phase: mobile phase A: 30% to 23% in a gradient, and mobile phase B: balance; when the elution time was 12-22 minutes, the volume concentration of the mobile phase: mobile phase A: 23%, and mobile phase B: balance; when the elution time was 22-22.2 minutes, the volume concentration of the mobile phase: mobile phase A: 23% to 30% in a gradient, mobile phase B: balance; when the elution time was 22.2-26 minutes, the volume concentration of the mobile phase: mobile phase A: 30%, and mobile phase B: balance.

[0055] Preparation of standard solution: standard solutions of CBDV, CBG, CBD, THC, and THCV with a concentration of 1.0 mg/ml were precisely measured, and respectively diluted in methanol into a standard solution with concentrations of CBDV, CBG, THC, and THCV being 10 μg/mL, and concentration of CBD being 150 μg/mL.

[0056] Preparation of waste liquid sample before degradation: 1.5 mL the reserved sample in step (1) was measured and placed in a 5 mL volumetric flask, dissolved and diluted to the calibration line with methanol, shaken well, filtered, and the filtrate was taken for detection and analysis.

[0057] Preparation of filtrate sample after degradation: 1.5 mL the filtrate obtained in step (3) was measured and placed in a 5 mL volumetric flask, dissolved and diluted to the calibration line with methanol, shaken well, filtered, and the filtrate was taken for detection and analysis.

[0058] 10 μL of the waste liquid sample before degradation and 10 μL of the filtrate sample after degradation were injected into high-performance liquid chromatography (HPLC), and chromatograms were recorded.

[0059] 3. Experimental Results

[0060] The chromatogram of standard solution in this example is shown in FIG. 1, and the chromatogram of the waste liquid sample before degradation is shown in FIG. 2, it can be obviously seen that the waste liquid sample before degradation contains CBDV, CBG, CBD, THC and THCV, especially contents of CBD, CBDV and THC are higher.

[0061] The chromatogram of the filtrate sample after degradation in this example is shown in FIG. 3. Compared with FIG. 1 and FIG. 2, the chromatographic peaks of CBG, CBD, and THCV substantially disappear, indicating that the remaining contents of CBG, CBD, and THCV were very small; and the chromatographic peaks of CBDV and THC are very narrow and low, indicating that the contents of CBDV and THC were low. According to the calculation, the total content of cannabinoids in the filtrate sample after degradation was 10 ppm. The experimental results show that the method for degrading cannabinoid in the invention can greatly degrade cannabinoids in waste liquid samples, and the total content of cannabinoids after degradation may reach ppm-level.

Example 2

[0062] 1. The Steps of the Method for Degrading Cannabinoid in the Example are as Follows:

[0063] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 70° C. to obtain a thick paste;

[0064] (2) the thick paste was added with ethyl acetate, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0065] (3) the enriched liquid of step (2) was placed in a closed treatment tank with illumination equipment, and 20% (mg/mL) of photocatalyst bismuth vanadate/bismuth oxychloride was added, and the resulting mixture was stirred at room temperature for 60 minutes with illuminance of 10000 Lx;

[0066] (4) the liquid after reaction in step (3) was left standing for 24 hours, and then filtered to obtain a filtrate and a filter cake;

[0067] (5) the filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 6 times. The finally collected filter cake was dried in a vacuum environment at 80° C. for 12 hours to obtain a recovered photocatalyst; and

[0068] (6) the reserved sample solution of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

[0069] 2. Methods of Sample Detection and Analysis

[0070] High-performance liquid chromatography detection conditions and system applicability test: octadecyl silane bonded silica gel was used as a filler, 0.1% formic acid in acetonitrile as mobile phase A, 0.1% formic acid aqueous solution as mobile phase B, the elution procedure is as follows, the detection wavelength was 220 nm, and the number of theoretical plates calculated by CBD was not less than 12000.

[0071] When the elution time was 0-6 minutes, the volume concentration of the mobile phase: mobile phase A: 30%, and mobile phase B: balance; when the elution time was 6-12 minutes, the volume concentration of the mobile phase: mobile phase A: 30% to 23% in a gradient, and mobile phase B: balance; when the elution time was 12-22 minutes, the volume concentration of the mobile phase: mobile phase A: 23%, and mobile phase B: balance; when the elution time was 22-22.2 minutes, the volume concentration of the mobile phase: mobile phase A: 23% to 30% in a gradient, and mobile phase B: balance; when the elution time was 22.2-26 minutes, the volume concentration of the mobile phase: mobile phase A: 30%, and mobile phase B: balance.

[0072] Preparation of standard solution: standard solutions of CBDV, CBG, CBD, THC, and THCV with a concentration of 1.0 mg/ml were precisely measured, and respectively diluted in methanol into a standard solution with concentrations of CBDV, CBG, THC, and THCV being 10 μg/mL, and concentration of CBD being 150 m/mL.

[0073] Preparation of waste liquid sample before degradation: 1.5 mL the reserved sample in step (1) was measured and placed in a 5 mL volumetric flask, dissolved and diluted to the calibration line with methanol, shaken well, filtered, and the filtrate was taken for detection and analysis.

[0074] Preparation of filtrate sample after degradation: 1.5 mL the filtrate obtained in step (3) was measured and placed in a 5 mL volumetric flask, dissolved and diluted to the calibration line with methanol, shaken well, filtered, and the filtrate taken for detection and analysis.

[0075] 10 μL of the waste liquid sample before degradation and 10 μL of the filtrate sample after degradation were injected into high-performance liquid chromatography (HPLC), and chromatograms were recorded.

[0076] 3. Experimental Results

[0077] According to calculation based on the chromatograms of the standard solution, the waste liquid sample before degradation and the filtrate sample after degradation in this example, the total content of cannabinoids in the filtrate sample after degradation was 8 ppm. The experimental results show that cannabinoid in a waste liquid sample can be greatly degraded by reasonably changing the amount of photocatalyst and the catalysis time according to the method for degrading cannabinoid of the invention, and the total content of cannabinoids after degradation may reach ppm-level.

Example 3

[0078] 1. The Steps of the Method for Degrading Cannabinoid in the Example are as Follows:

[0079] (1) reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 40° C. to obtain a thick paste;

[0080] (2) the thick paste was added with acetone, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0081] (3) the enriched liquid of step (2) was placed in a closed treatment tank with illumination equipment, and 30% (mg/mL) of example 1 recovered bismuth vanadate photocatalyst was added, and the resulting mixture was stirred at room temperature for 60 minutes with illuminance of 4500 Lx;

[0082] (4) the liquid after reaction in step (3) was left standing for 78 hours, and then filtered to obtain a filtrate and a filter cake;

[0083] (5) the filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 2 times. The finally collected filter cake was dried in a vacuum environment of 80° C. for 12 hours to obtain a recovered photocatalyst; and

[0084] (6) the reserved sample of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

[0085] 2. Methods of Sample Detection and Analysis

[0086] The methods of sample detection and analysis of this example were the same as those of Example 2.

[0087] 3. Experimental Results

[0088] According to calculation based on the chromatograms of the standard solution, the waste liquid sample before degradation, and the filtrate sample after degradation in this example, the total content of cannabinoids in the filtrate sample after degradation was 10 ppm. The experimental results show that the recovered photocatalyst used in the method for degrading cannabinoid of the invention can still greatly degrade cannabinoids in waste liquid samples, and the total content of cannabinoids can still reach ppm-level after degradation.

Example 4

[0089] 1. The Steps of the Method for Degrading Cannabinoid in the Example are as Follows:

[0090] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 60° C. to obtain a thick paste;

[0091] (2) the thick paste was added with methanol, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0092] (3) the enriched liquid in step (2) was placed in a closed treatment tank with illumination equipment, and 20% (mg/mL) of photocatalyst bismuth oxychloride was added, and the resulting mixture was stirred at room temperature for 5 minutes with illuminance of 20000 Lx;

[0093] (4) the liquid after reaction in step (3) was left standing for 50 hours, and then filtered to obtain a filtrate and a filter cake;

[0094] (5) the filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 3 times. The finally collected filter cake was dried in a vacuum environment at 80° C. for 12 hours to obtain a recovered photocatalyst; and

[0095] (6) the reserved sample of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

[0096] 2. Methods of Sample Detection and Analysis

[0097] The methods of sample detection and analysis of this example were the same as those of Example 2.

[0098] 3. Experimental Results

[0099] According to calculation based on the chromatograms of the standard solution, the waste liquid sample before degradation, and the filtrate sample after degradation in this example, the total content of cannabinoids in the filtrate sample after degradation was 15 ppm. The experimental results show that the method for degrading cannabinoid of the invention can greatly degrade cannabinoids in waste liquid samples, and the total content of cannabinoids after degradation may reach ppm-level.

Example 5

[0100] 1. The Steps of the Method for Degrading Cannabinoid in the Example are as Follows:

[0101] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 60° C. to obtain a thick paste;

[0102] (2) the thick paste was added with 95% ethanol, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0103] (3) the enriched liquid in step (2) was placed in a closed treatment tank with illumination equipment, and 15% (mg/mL) of photocatalyst bismuth vanadate/titanium dioxide was added, and the resulting mixture was stirred at room temperature for 60 minutes with illuminance of 20000 Lx;

[0104] (4) the liquid after reaction in step (3) was left standing for 24 hours, and then filtered to obtain a filtrate and a filter cake;

[0105] (5) the filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes, and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake, the finally collected filter cake was dried in a vacuum environment at 80° C. for 12 hours to obtain a recovered photocatalyst; and

[0106] (6) the reserved sample of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

[0107] 2. Methods of Sample Detection and Analysis

[0108] The methods of sample detection and analysis of this example were the same as those of Example 2.

[0109] 3. Experimental Results

[0110] According to calculation based on the chromatograms of the standard solution, the waste liquid sample before degradation, and the filtrate sample after degradation in this example, the total content of cannabinoids in the filtrate sample after degradation was 12 ppm. The experimental results show that the method for degrading cannabinoid of the invention can greatly degrade cannabinoids in waste liquid samples, and the total content of cannabinoids after degradation may reach ppm-level.

Example 6

[0111] 1. The Steps of the Method for Degrading Cannabinoid in the Example are as Follows:

[0112] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 60° C. to obtain a thick paste;

[0113] (2) the thick paste was added with 95% ethanol, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0114] (3) the enriched liquid in step (2) was placed in a closed treatment tank with illumination equipment, and 5% (mg/mL) of photocatalyst bismuth vanadate was added, and the resulting mixture was stirred at room temperature for 60 minutes with illuminance of 12000 Lx;

[0115] (4) the liquid after reaction in step (3) was left standing for 78 hours, and then filtered to obtain a filtrate and a filter cake;

[0116] (5) the filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes, and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 3 times. The finally collected filter cake was dried in a vacuum environment at 80° C. for 12 hours to obtain a recovered photocatalyst; and

[0117] (6) the reserved sample of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

[0118] 2. Methods of Sample Detection and Analysis

[0119] The methods of sample detection and analysis of this example were the same as those of Example 2.

[0120] 3. Experimental Results

[0121] According to calculation based on the chromatograms of the standard solution, the waste liquid sample before degradation, and the filtrate sample after degradation in this example, the total content of cannabinoids in the filtrate sample after degradation was 13 ppm. The experimental results show that the method for degrading cannabinoid of the invention can greatly degrade cannabinoids in waste liquid samples, and the total content of cannabinoids after degradation may reach ppm-level.

Example 7

[0122] The amount of photocatalyst used in this example was 10% (mg/mL), and other experimental and detection conditions were the same as those in Example 6.

Example 8

[0123] The amount of photocatalyst used in this example was 20% (mg/mL), and other experimental and detection conditions were the same as those in Example 6.

Example 9

[0124] The amount of photocatalyst used in this example was 30% (mg/mL), and other experimental and detection conditions were the same as those in Example 6.

TABLE-US-00001 TABLE 1 Comparison of the amount of photocatalyst in Example 6-9 Exam- Exam- Exam- Exam- ple 6 ple 7 ple 8 ple 9 Amount of photocatalyst (mg/mL) 5% 10% 20% 30% Total cannabinoid content 13 8 10 14 in filtrate sample after degradation (ppm)

[0125] In Table 1, the amount of photocatalyst used in Example 6-9 was 5%, 10%, 10% and 30% (mg/mL), respectively, under the same other experimental conditions, the total content of cannabinoids in the filtrate samples after degradation were 13, 8, 10, and 14 ppm, respectively, indicating that the degradation of cannabinoids is better when the amount of photocatalyst is 10% (mg/mL).

Example 10

[0126] The photocatalyst of this example was bismuth vanadate/bismuth oxychloride composite, and other experimental and detection conditions were the same as those of Example 7.

Example 11

[0127] The photocatalyst of this example was bismuth oxychloride, and other experimental and detection conditions were the same as those of Example 7.

Example 12

[0128] The photocatalyst of this example was bismuth vanadate/titanium dioxide composite, and other experimental and detection conditions were the same as those of Example 7.

Example 13

[0129] The photocatalyst of this example was bismuth vanadate/titanium dioxide composite, and other experimental and detection conditions were the same as those of Example 7.

TABLE-US-00002 TABLE 2 Comparison of the types of photocatalysts in Example 7, 10-13 Exam- Exam- Exam- Exam- Exam- ple7 ple10 ple11 ple12 ple13 Total cannabinoid 8 8.1 16 14 20 content in filtrate sample after degradation (ppm)

[0130] In table 2, the photocatalysts of Examples 7, 10-13 were bismuth vanadate, bismuth vanadate/bismuth oxychloride composite, bismuth vanadate/titanium dioxide composite, and bismuth vanadate/titanium dioxide composite, respectively, under the same other experimental conditions, the total content of cannabinoids in the filtrate samples after degradation were 8, 8.1, 16, 14, and 20 ppm, respectively, indicating that the degradation of cannabinoids is better when the photocatalysts is bismuth vanadate or bismuth vanadate/bismuth oxychloride respectively.

Example 14

[0131] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 55° C. to obtain a thick paste;

[0132] (2) the thick paste was added with 95% ethanol, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0133] (3) the enriched liquid in step (2) was placed in a closed treatment tank with illumination equipment, and 18% (mg/mL) of photocatalyst bismuth vanadate was added, and the resulting mixture was stirred at room temperature for 40 minutes with illuminance of 5500 Lx;

[0134] (4) the liquid after reaction in step (3) was left standing for 20 hours, and then filtered to obtain a filtrate and a filter cake;

[0135] (5) the filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 3 times. The finally collected filter cake was dried in a vacuum environment at 65° C. for 20 hours to obtain a recovered photocatalyst; and

[0136] (6) the reserved sample of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

Example 15

[0137] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 65° C. to obtain a thick paste;

[0138] (2) the thick paste was added with 95% ethanol, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0139] (3) the enriched liquid in step (2) was placed in a closed treatment tank with illumination equipment, and 23% (mg/mL) of photocatalyst bismuth vanadate/bismuth oxychloride composite was added, and the resulting mixture was stirred at room temperature for 15 minutes with illuminance of 8300 Lx;

[0140] (4) the liquid after reaction in step (3) was left standing for 35 hours, and then filtered to obtain a filtrate and a filter cake;

[0141] (5) The filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 3 times. The finally collected filter cake was dried in a vacuum environment at 70° C. for 18 hours to obtain a recovered photocatalyst; and

[0142] (6) the reserved sample of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

Example 16

[0143] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 45° C. to obtain a thick paste;

[0144] (2) the thick paste was added with 95% ethanol, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0145] (3) the enriched liquid in step (2) was placed in a closed treatment tank with illumination equipment, and 35% (mg/mL) of photocatalyst bismuth oxychloride was added, and the resulting mixture was stirred at room temperature for 10 minutes with illuminance of 3800 Lx;

[0146] (4) the liquid after reaction in step (3) was left standing for 65 hours, and then filtered to obtain a filtrate and a filter cake;

[0147] (5) the filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 3 times. The finally collected filter cake was dried in a vacuum environment at 55° C. for 24 hours to obtain a recovered photocatalyst; and

[0148] (6) the reserved solution of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

Example 17

[0149] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 60° C. to obtain a thick paste;

[0150] (2) the thick paste was added with 95% ethanol, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0151] (3) the enriched liquid in step (2) was placed in a closed treatment tank with illumination equipment, and 42% (mg/mL) of photocatalyst bismuth vanadate/bismuth oxychloride composite was added, and the resulting mixture was and stirred at room temperature for 55 minutes with illuminance of 7200 Lx;

[0152] (4) the liquid after reaction in step (3) was left standing for 18 hours, and then filtered to obtain a filtrate and a filter cake;

[0153] (5) the filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was added into ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 3 times. The finally collected filter cake was dried in a vacuum environment at 75° C. for 15 hours to obtain a recovered photocatalyst; and

[0154] (6) the reserved sample of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

Example 18

[0155] (1) after reserving a sample from a waste liquid containing cannabinoid, the remaining waste liquid was concentrated under reduced pressure at 53° C. to obtain a thick paste;

[0156] (2) the thick paste was added with 95% ethanol, stirred for dissolution to obtain an enriched liquid of the waste liquid containing cannabinoid;

[0157] (3) the enriched liquid in step (2) was placed in a closed treatment tank with illumination equipment, and 7% (mg/mL) of photocatalyst bismuth vanadate/titanium dioxide composite was added, and the resulting mixture was stirred at room temperature for 25 minutes with illuminance of 10500 Lx;

[0158] (4) the liquid after reaction in step (3) was left standing for 40 hours, and then filtered to obtain a filtrate and a filter cake;

[0159] (5) the filter cake obtained in step (4) was added into water, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was added into 95% ethanol, and the resulting mixture was stirred for 15 minutes and then filtered to collect a filter cake. The resulting filter cake was washed repeatedly for 3 times. The finally collected filter cake was dried in a vacuum environment of 80° C. at 12 hours to obtain a recovered photocatalyst; and

[0160] (6) the reserved sample of step (1) and the filtrate obtained in step (4) were analyzed by high-performance liquid chromatography (HPLC) to detect the contents of CBDV, CBD, CBG, THCV, and THC.

[0161] The above are only preferred examples of the invention and are not intended to limit the invention. Any modifications, equivalent replacements, etc. made within the spirit and principles of the invention shall be included in the protection scope of the invention.