METHOD FOR MICROSCOPIC EXAMINATION OF PHYTOPLANKTON

Abstract

The present disclosure relates to a method for microscopic examination of phytoplankton. The present disclosure solves the problem of decreased counting accuracy of perioptometry in a dry environment. The method is as follows: step 1, making an analyte into a glass slide, performing qualitative identification of the phytoplankton in the field of view; and step 2, drying the glass slide; after drying, counting the phytoplankton and full-slide rare algae on the glass slide microscopically. When the humidity of microscopy room is only 20%, the method of the present disclosure may count a sample accurately, which has a significant increase in counting accuracy compared with the existing perioptometry. The method of the present disclosure effectively solves the problem of failure to perform the perioptometry accurately due to dry ambient humidity.

Claims

1. A method for microscopic examination of phytoplankton, comprising the following steps: step 1, making an analyte into a glass slide, performing qualitative identification of the phytoplankton in the field of view, and determining dominant species, common species, and rare species of a sample to be counted; and step 2, drying the glass slide in step 1; after drying, counting the phytoplankton and full-slide rare algae on the glass slide microscopically, namely realizing the microscopic examination of the phytoplankton.

2. The method according to claim 1, wherein the drying in step 2 is carried out by using a dryer, a fan, or a blower.

Description

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0011] Embodiment 1: A method for microscopic examination of phytoplankton in this example includes the following steps:

[0012] step 1, making an analyte into a glass slide, performing qualitative identification of the phytoplankton in the field of view, and determining dominant species, common species, and rare species of a sample to be counted; and

[0013] step 2, drying the glass slide in step 1; after drying, counting the phytoplankton and full-slide rare algae on the glass slide microscopically, namely realizing the microscopic examination of the phytoplankton.

[0014] In the embodiment, in the drying process of step 2, as drying a liquid sample is accelerated by external force, the glass slide is not allowed to face toward an air outlet position directly; furthermore, excessive wind must be avoided, and massive displacement of the algae distributed on the glass slide is avoided due to external force, for fear of the aggregation of the algae on the glass slide.

[0015] Embodiment 2: The difference between this embodiment and Embodiment 1 is that: a dryer, a fan, or a blower is used in the drying in step 2. Other steps and parameters are the same as those in Embodiment 1.

[0016] In this embodiment, in the drying process, as drying a liquid sample is accelerated by external force, particularly by a fan or a blower, the glass slide is not allowed to face toward an air outlet position directly; furthermore, excessive wind must be avoided, and massive displacement of the algae distributed on the glass slide is avoided due to external force, for fear of the aggregation of the algae on the glass slide.

EXAMPLE 1

[0017] The phytoplankton in samples of the freshwater reservoir Songhua Lake in August 2020 were counted by the perioptometry and the method of the present disclosure. Herein, the method for microscopic examination of phytoplankton provided by the present disclosure included the following steps:

[0018] step 1, an analyte was made into a glass slide, qualitative identification of the phytoplankton was performed in the field of view, and dominant species, common species, and rare species of a sample to be counted was determined; and

[0019] step 2, the glass slide in step 1 was dried in a dryer; after drying, the phytoplankton and full-slide rare algae were counted on the glass slide microscopically by using perioptometry.

[0020] The counting results were shown in Table 1. Herein, samples 1 and 2 were counted in a microscopy room with a humidity of 20%.

EXPERIMENTAL COMPARISON RESULTS

[0021]

TABLE-US-00001 Present Present Perioptometry disclosure Perioptometry disclosure (algae) Algae of sample 2 (algae) (algae) Algae of sample 1 (algae) 162 Fragilaria sp. 604 290 Fragilaria sp. 632 14 Synedra sp. 35 17 Synedra sp. 57 0 Stephanodiscus sp. 2 0 Stephanodiscus sp. 1 0 Cyclotella sp. 8 2 Cyclotella sp. 1 0 Melosira granulata (Ehr.) Ralfs 30 16 Melosira granulata (Ehr.) Ralfs 1 14 Microcystis sp. 259 22 Microcystis sp. 21 0 Raphidiopsis sp. 48 24 Aphanocapsa sp. 4 0 Scenedesmus arcuatus 9 2 Raphidiopsis sp. 4 0 Scenedesmus bijuga 130 28 Anabaena circinalis 8 2 Chlorella sp. 4 0 Ankistrodesmus sp. 4 0 Oocystis sp. 16 4 Chroomonas sp. 2 0 Ceratium hirundinella 10 2 Cyrptomonas ovata 3 0 Cosmarium sp. 41 15 Chlorella sp. 2 1 Chroomonas sp. 8 0 Scenedesmus bijuga 4 0 Cryptomonas ovata 16 0 Scenedesmus obliquus 2

[0022] According to the experimental results in Table 1, when the humidity of the microscopy room is only 20%, the method of the present disclosure can accurately count the samples, which has an increase in counting accuracy compared with the existing perioptometry. The counting method of phytoplankton samples provided by the present disclosure effectively solves the problem of failure to perform the perioptometry accurately due to dry ambient humidity.