Extracellular heme production method using metabolically engineered microorganism
11371068 · 2022-06-28
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Cpc classification
C12P17/182
CHEMISTRY; METALLURGY
C12P13/005
CHEMISTRY; METALLURGY
C12Y402/01075
CHEMISTRY; METALLURGY
C12N9/0008
CHEMISTRY; METALLURGY
C12N15/67
CHEMISTRY; METALLURGY
C12Y401/01037
CHEMISTRY; METALLURGY
C12Y603/05007
CHEMISTRY; METALLURGY
C12Y504/03008
CHEMISTRY; METALLURGY
International classification
C12N1/22
CHEMISTRY; METALLURGY
C12P13/00
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C12N15/67
CHEMISTRY; METALLURGY
C12N9/00
CHEMISTRY; METALLURGY
Abstract
The present invention relates to a microorganism variant having the ability to extracellularly produce heme, and more particularly to a metabolically engineered microorganism variant having the ability to extracellularly produce heme and a method of producing heme using the same. According to the present invention, heme, an organometallic compound which is increasingly used as a health food or food supplement for the treatment of porphyria, can be extracellularly secreted and produced in high yield using the microorganism variant, but not conventional chemical synthesis or enzymatic synthesis.
Claims
1. A metabolically engineered variant microorganism that secretes heme extracellularly, wherein the microorganism is modified by the introduction or overexpression of ccmABC genes encoding a heme exporter, wherein the microorganism comprises genes in a C5 biosynthetic pathway that produces 5-aminolevulinate (ALA) from glutamate, and genes in a pathway that synthesizes heme from ALA, wherein the genes in the C5 biosynthetic pathway comprise a gltX gene encoding glutamyl-tRNA synthase (GIuRS), a hemA gene encoding glutamyl-tRNA reductase (GluTR), and a hemL gene encoding glutamate-1-semialdehyde 2,1-aminomutase (GSAM), wherein the genes in the pathway that synthesizes heme from ALA comprise a hemB gene encoding porphobilinogen synthase (PBGS), a hemC gene encoding porphobilinogen deaminase (PBGD), a hemD gene encoding uroporphyrinogen III synthase (UROS), a hemE gene encoding uroporphyrinogen III decarboxylase (UROD), a hemF gene encoding coproporphyrinogen III oxidase (CPO), a hemG gene encoding protoporphyrinogen oxidase (PPO), and a hemH gene encoding ferrochelatase (FECH), and wherein the microorganism is selected from the group consisting of Escherichia coli, Bacillus sp., Corynebacterium sp., Lactobacillus sp., Lactococcus sp., Pseudomonas sp., Anacystis sp., Anabaena sp., Chlorobium sp., Chloroflexus sp., Clostridium sp., Methanobacteria, Propionibacterium sp., Rhodopseudomonas sp., Rhodobacter sp., Rhodovulum sp., Streptococcus sp., Saccharomyces sp., Schizosaccharomyces sp., Yarrowia sp., and Aspergillus sp.
2. The metabolically engineered variant microorganism of claim 1, wherein the microorganism in the presence of a carbon source produces glutamic acid.
3. The metabolically engineered variant microorganism of claim 1, wherein the genes in the C5 biosynthetic pathway that produces 5-aminolevulinate (ALA) from glutamate, and the genes in the pathway that synthesizes heme from ALA, are overexpressed.
4. The metabolically engineered variant microorganism of claim 1, wherein the variant microorganism is further modified by deletion of a yfeXgene.
5. A method for producing heme comprising the steps of: (a) culturing the metabolically engineered variant microorganism of claim 1 to produce heme; and (b) recovering the produced heme.
6. The method of claim 5, wherein step (a) further comprises supplying a nitrogen source in the culturing.
7. A metabolically engineered variant microorganism that extracellularly secretes heme, wherein the microorganism is modified to overexpress ccmABC genes encoding a heme exporter, wherein the microorganism comprises genes in a C5 biosynthetic pathway that produces 5-aminolevulinate (ALA) from glutamate, and genes in a pathway that synthesizes heme from ALA, wherein at least one of the genes in the C5 biosynthetic pathway that produces 5-aminolevulinate (ALA) from glutamate and at least one of the genes in the pathway that synthesizes heme from ALA, are overexpressed; wherein the genes in the C5 biosynthetic pathway comprise at least one gene selected from: a gltX gene encoding glutamyl-tRNA synthase (GIuRS), a hemA gene encoding glutamyl-tRNA reductase (GIuTR), and a hemL gene encoding glutamate-1-semialdehyde 2,1-aminomutase (GSAM); wherein the genes in the pathway that synthesizes heme from ALA comprise at least one gene selected from: a hemB gene encoding porphobilinogen synthase (PBGS), a hemC gene encoding porphobilinogen deaminase (PBGD), a hemD gene encoding uroporphyrinogen III synthase (UROS), a hemE gene encoding uroporphyrinogen III decarboxylase (UROD), a hemF gene encoding coproporphyrinogen III oxidase (CPO), a hemG gene encoding protoporphyrinogen oxidase (PPO), and a hemH gene encoding ferrochelatase (FECH); and wherein the microorganism is further modified by deletion of a yfeX gene.
8. The metabolically engineered variant microorganism of claim 7, wherein the microorganism is selected from the group consisting of Escherichia coli, Bacillus sp., Corynebacterium sp., Lactobacillus sp., Lactococcus sp., Pseudomonas sp., Anacystis sp., Anabaena sp., Chlorobium sp., Chloroflexus sp., Clostridium sp., Methanobacteria, Propionibacterium sp., Rhodopseudomonas sp., Rhodobacter sp., Rhodovulum sp., Streptococcus sp., Saccharomyces sp., Schizosaccharomyces sp., Yarrowia sp., and Aspergillus sp.
9. The metabolically engineered variant microorganism of claim 1, wherein the microorganism is Corynebacterium glutamicum, and wherein the microorganism is modified to overexpress the hemA gene and the hemL gene in said the C5 biosynthetic pathway.
10. The metabolically engineered variant microorganism of claim 1, wherein the microorganism is Corynebacterium glutamicum, and wherein the microorganism is modified to overexpress the hemB gene and the hemH gene in the pathway that synthesizes heme from ALA.
11. The metabolically engineered variant microorganism of claim 1, wherein the microorganism is Corynebacterium glutamicum, and wherein the microorganism is modified to overexpress the hemA gene and the hemL gene in the C5 biosynthetic pathway, and to overexpress the hemB gene and the hemH gene in the pathway that synthesizes heme from ALA.
12. The metabolically engineered variant microorganism of claim 1, wherein the heme secreted extracellularly comprises free heme.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
(1) The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
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BEST MODE FOR CARRYING OUT THE INVENTION
(11) Unless defined otherwise, all the technical and scientific terms used herein have the same meaning as those generally understood by one of ordinary skill in the art to which the invention pertains. Generally, the nomenclature used herein and the experiment methods, which will be described below, are those well known and commonly employed in the art.
(12) Heme is an organometallic compound which is widely used in the health care and food supplement industries. In a conventional art, heme was produced by chemical synthesis, organic extraction or enzymatic hydrolysis, but this method is complicated and has a low yield. For this reason, an E. coli variant was constructed to produce heme in high yield. In addition, an E. coli variant was constructed which secretes produced heme extracellularly, making it easy to recover free heme later, unlike a conventional E. coli strain that accumulates produced heme in the cells.
(13) Heme is an essential compound for microorganisms, but when heme is present at high concentrations, it causes toxicity. Thus, some microorganisms acquire heme resistance by actively transporting heme or toxic metabolites that are generated by heme accumulation to the outside of the cells. In the case of E. coli, the genes ccmABC are known to encode a heme exporter that supplies heme to proteins involved in cytochrome c biosynthesis (Feissner et al., Mol. Microbiol. 61:219-231, 2006). However, it has not been reported whether the gene is involved in resistance to toxicity caused by free heme. Nevertheless, the present inventors have determined that enhancement of the biosynthesis of free heme can promote the extracellular secretion of heme by the heme exporter expressed from the ccmABC genes, thereby enabling extracellular production of free heme.
(14) A conventional heme-producing variant using E. coli was designed to produce heme from 5-aminolevulinic acid via the C4 pathway together with the supply of L-glycine and succinate, but the final titer of heme in this variant was very low. To more efficiently produce heme, the present inventors have constructed a metabolically engineered E. coli strain utilizing the C5 pathway that converts L-glutamate to 5-aminolevulinic acid, and have increased the production of heme in E. coli by using various strategies (
(15) First, the capacities of the C4 and C5 pathways to produce ALA without feeding precursors were examined. After confirming the superior performance of the C5 pathway over that of the C4 pathway, the metabolic genes of the C5 pathway and downstream pathways for heme biosynthesis were overexpressed. Then, the metabolic pathways associated with heme biosynthesis were optimized by adjusting the expression levels of the relevant genes. To further increase the production of heme, the yfeX gene, which is suspected to encode a heme-degrading enzyme, and lactate and acetate biosynthetic pathways were deleted, and it was confirmed that heme was produced in high titer by batch fermentation. In addition, it was confirmed that the produced heme was secreted extracellularly.
(16) Furthermore, an E. coli variant overexpressing the ccmABC genes encoding the heme exporter that secretes heme extracellularly was constructed, and it was confirmed that heme was produced with higher efficiency by batch fermentation. In addition, it was confirmed that a higher proportion of the produced heme was secreted extracellularly.
(17) Therefore, the present invention is directed to a microorganism variant capable of secreting heme extracellularly in which the genes encoding a heme exporter is overexpressed in a microorganism having genes involved in biosynthesis of 5-aminolevulinate (ALA), and genes involved in biosynthesis of heme from ALA.
(18) In the present invention, the microorganism having genes involved in biosynthesis of 5-aminolevulinate (ALA) and genes involved in biosynthesis of heme from ALA includes a microorganism introduced with such genes as well as the microorganism naturally having the genes.
(19) In the present invention, the microorganism variant may be characterized in that the genes involved in biosynthesis of 5-aminolevulinate (ALA) and the genes involved in biosynthesis of heme from ALA are overexpressed.
(20) The precursor 5-aminolevulinate (ALA) is required to biosynthesize heme, and both the C4 pathway and the C5 pathway can supply ALA. Although examples were reported in which both the C4 pathway and the C5 pathway were used to produce ALA, the present invention is the first to apply the C5 pathway to variants for biosynthesis of heme.
(21) The microorganism that can be used in the present invention may be a microorganism capable of producing glutamic acid from a carbon source, but is not limited thereto.
(22) Conventional microorganisms having the ability to biosynthesize heme using the C4 pathway had problems in that AIA precursors (succinate and glycine) need to be added during culture and it is not permitted to add glycine at high concentration, due to its cytotoxicity. In addition, even when succinate and glycine were added, the final titer of heme was no higher than about 6.4 mg/L.
(23) In the present invention, in order to construct a microorganism variant having a high ability to produce heme, microorganism variants having the C4 pathway and the C5 pathway, respectively, were constructed, and their abilities to produce heme were examined.
(24) Examples of the microorganism variants that can be used in the present invention may include bacteria, archaea, yeasts, fungi, protozoa (flagellate, amoebozoa, choanoflagellate, rhizaria, chromalveolata), animal cells, microalgae, and plant cells, more preferably, Escherichia coli, Bacillus sp., Corynebacterium sp., Lactobacillus sp., Lactococcus sp., Pseudomonas sp., Anacystis sp., Anabaena sp., Chlorobium sp., Chloroflexus sp., Clostridium sp., Methanobacteria, Propionibacterium sp., Rhodopseudomonas sp., Rhodobacter sp., Rhodovulum sp., Streptococcus sp., Saccharomyces sp., Schizosaccharomyces sp., Yarrowia sp., and Aspergillus sp., further preferably, Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Lactobacillus brevis, Lactobacillus casei, Lactobacillus reuteri, Lactococcus lactis, Aspergillus niger, Saccharomyceses cerevisiae, and Saccharomyces pombe, but are not limited thereto.
(25) In one example of the present invention, when E. coli strains introduced with the C4 pathway and the C5 pathway, respectively, were cultured without adding the precursors, more ALA was produced in the C5 pathway. Thus, in the present invention, the C5 pathway was used for heme biosynthesis.
(26) In the present invention, the genes involved in the C5 biosynthetic pathway may be selected from the group consisting of gltX encoding glutamyl-tRNA synthase (GluRS), hemA encoding glutamyl-tRNA reductase (GluTR), and hemL encoding glutamate-1-semialdehyde 2,1-aminomutase (GSAM), and the gene involved in a pathway that synthesizes heme from ALA may be selected from the group consisting of hemB encoding porphobilinogen synthase (PBGS), hemC encoding porphobilinogen deaminase (PBGD), hemD encoding uroporphyrinogen III synthase (UROS), hemE encoding uroporphyrinogen III decarboxylase (UROD), hemF encoding coproporphyrinogen III oxidase (CPO), hemG encoding protoporphyrinogen oxidase (PPO), and hemH encoding ferrochelatase (FECH).
(27) In the present invention, the variant may be characterized in that a gene encoding a heme exporter is overexpressed.
(28) In the present invention, the variant may lack a gene that encodes a heme or heme intermediate-degrading enzyme, and the gene that encodes a heme or heme intermediate-degrading enzyme may be the yfeX gene.
(29) In another example, an E. coli variant was constructed by co-overexpressing the genes of the heme biosynthetic pathway in an E. coli strain with the C5 pathway genes overexpressed, optimizing a vector used for expression of each enzyme, deleting genes involved in acetate and lactate production, and deleting the yfeX gene of which involvement in degradation of heme has been controversial. This E. coli variant produced 7.13 mg/L of heme in flask culture using LB medium supplemented with 7.5 mg/L of FeSO.sub.47H.sub.2O.
(30) Therefore, in the present invention, it was first confirmed that the knockout of the yfeX gene increases the production of heme.
(31) Another aspect of the present invention is directed to a variant in which the yfeX gene is deleted in a microorganism in which the C4 pathway of ALA is overexpressed.
(32) In addition, in another example of the present invention, the E. coli variant strain constructed as described above was flask-cultured in an MR medium containing 5 g/L of yeast extract and a FeSO.sub.47H.sub.2O concentration adjusted to 7.5 mg/L, and as a result, 7.45 mg/L of heme was produced. Furthermore, the optimization of culture time and temperature was performed, protein overexpression was induced by IPTG, and then culture was performed at 30° C. for 60 hours, and as a result, 7.78 mg/L of heme was produced.
(33) In still another example of the present invention, batch fermentation was performed using the above-described E. coli variant under optimized conditions, and as a result, a total of 14.24 mg/L of heme was produced at 56 hours of culture, and 1.18 mg/L (8.29%) of the produced heme was found extracellularly.
(34) In still another example of the present invention, the E. coli variant was cultured in a fed-batch culture process, and as a result, 49.18 mg/L of heme was produced at 56 hours of culture, and 16.77 mg/L (34.10%) of the produced heme was found extracellularly.
(35) In yet another example of the present invention, fed-batch culture was performed using a medium supplemented with (NH.sub.4).sub.2SO.sub.4 in order to overcome concerns about nitrogen deficiency, because 4 nitrogen (N) atoms were required per mole of heme, and as a result, 104.90 mg/L of heme was produced. 54.61 mg/L (52.06%) of the produced heme was found extracellularly.
(36) In yet another example of the present invention, fed-batch culture was performed using a medium supplemented with 1-glutamate, a precursor of the C5 ALA biosynthesis pathway instead of (NH.sub.4).sub.2SO.sub.4, and as a result, 228.46 mg/L of heme was produced at 64 hours of culture, and 131.90 mg/L (57.734%) of the produced heme was found extracellularly.
(37) Considering the above results, it can be seen that the proportion of extracellularly secreted heme increases as the total amount of heme produced increases. Therefore, it was concluded that, because a high intracellular concentration of heme is cytotoxic, the function or expression of the heme exporter could be promoted, like the hypothesis established by the present inventors. Thus, it was concluded that when the ccmABC gene known to encode the heme exporter is overexpressed, the extracellular secretion of heme will further be promoted, and as a result, toxicity caused by intracellular free heme will decrease and the production of heme will also increase. Based on this conclusion, a method for overexpressing the ccmABC genes was attempted.
(38) Therefore, in another aspect, the present invention is directed to a method for producing heme comprising the steps of: culturing the microorganism variant capable of secreting heme extracellularly, in which genes involved in a C5 biosynthetic pathway that produces 5-aminolevulinate (ALA), and genes involved in a pathway that synthesizes heme from ALA are overexpressed and genes encoding heme exporter is overexpressed, to produce heme; and recovering the produced heme.
(39) In one example of the present invention, a strain constructed by overexpressing the heme exporter-encoding ccmABC genes in the above-described heme-producing E. coli strain was cultured in a fed-batch process in the presence of (NH.sub.4).sub.2SO.sub.4, and as a result, 114.79 mg/L of heme was produced at 64 hours of culture, and 71.91 mg/L (62.64%) of the produced heme was found extracellularly.
(40) In another example of the present invention, the strain of the present invention was cultured in a fed-batch process in the presence of glutamate, and as a result, 246.69 mg/L of heme was produced at 72 hours of culture, and 164.12 mg/L (66.53%) of the produced heme was found extracellularly.
(41) When heme was produced using the microorganism variant of the present invention, in one example, heme was produced in an amount of 114.79 mg/L or 246.69 mg/L, depending on whether the precursors were added. This amount corresponds to a 17.9-fold higher or 38.5-fold higher yield than that of a conventional heme-producing E. coli variant that produced 6.4 mg/L of heme.
(42) In addition, in the present invention, it was first confirmed that as the amount of heme produced by a microorganism increases, the proportion of extracellular heme also increases. Furthermore, it was first confirmed that when the heme exporter genes ccmABC are overexpressed, the proportion of extracellular heme increases and the total amount of heme produced by the cells also increases.
(43) Hereinafter, the present invention will be described in further detail with reference to examples. It will be obvious to a person having ordinary skill in the art that these examples are for illustrative purposes only and are not to be construed to limit the scope of the present invention.
(44) The restriction enzymes used in these examples and the following examples were purchased from New England Biolabs (USA) and Enzynomics (Korea), and the PCR polymerase was purchased from BIOFACT (Korea), and the DNA ligase was purchased from Elpis Biotech (Korea). Others were indicated separately.
Example 1: Selection of an ALA Biosynthetic Pathway for Highly Efficient Production of Heme
1-1: Construction of Vectors (pRSF-C4 and pRSF-05) Expressing C4 and C5 ALA Biosynthetic Genes
(45) pRSF-C4 and pRSF-05 vectors capable of expressing C4 and C5 ALA biosynthetic genes in the E. coli strain BL21(DE3) (Studier et al., J. Mol. Biol. 189:113-130, 1986) were constructed through the following procedures.
(46) The plasmid pRSF-C4 capable of expressing the C4 ALA biosynthetic genes was constructed by inserting an aminolevulinic acid synthase (ALAS) gene (hemA.sub.Rsp) derived from Rhodobacter sphaeroides 2.4.1 and maeB and coaA genes derived from E. coli into a plasmid pRSFDuet-1 (Novagen, USA). To amplify the hemA.sub.Rsp gene derived from Rhodobacter sphaeroides 2.4.1, the hemA.sub.Rsp gene (SEQ ID NO: 1) codon-optimized according to the codon usage of E. coli was synthesized (Genotech, Korea), thereby preparing a template, and PCR was performed using the template and the primers of SEQ ID NOs: 2 and 3. The maeB gene (SEQ ID NO: 4) and coaA gene (SEQ ID NO: 5) derived from E. coli were amplified using the genomic DNA of E. coli BL21(DE3) as a template and also using the primers of SEQ ID NOs: 6 and 7 and the primers of SEQ ID NOs: 8 and 9, respectively. At this time, the sequence of a ribosome binding site (RBS) was added to the 5′ end of the amplified coaA gene by the sequence of the ribosome binding site (RBS) contained in the primer of SEQ ID NO: 7 used in the amplification process.
(47) TABLE-US-00001 TABLE 1 SEQ ID NOs Nucleotide sequences SEQ ID 5′-CATGCCATGG ATTATAACCT GGCACTGG-3′ NO: 2 SEQ ID 5′-CCGGAATTCT TAGGCAACCA CTTCCGC-3′ NO: 3 SEQ ID 5′-GAAGATCTAT GGATGACCAG TTAAAACAAA G-3′ NO: 6 SEQ ID 5′-CCGCTCGAGT TACAGCGGTT GGGTTTG-3′ NO: 7 SEQ ID 5′-CGAGCTCATA AAAGGAGGAA AATATATGAG NO: 8 TATAAAAGAG CAAACGTT-3′ SEQ ID 5′-ACGCGTCGAC TTATTTGCGT AGTCTGACCT NO: 9 CT-3′
(48) The amplified sequences were cleaved with BglII and XhoI, SacI and SalI, and NcoI and EcoRI, respectively, and then sequentially inserted into a pRSFDuet-1 vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pRSF-C4.
(49) The vector pRSF-C5 capable of expressing the C5 ALA biosynthetic genes was constructed by inserting the gltX gene (SEQ ID NO: 10), hemL gene (SEQ ID NO: 11) and negative feedback resistance hemA (hemA.sup.fbr) gene (SEQ ID NO: 12), which are derived from E. coli. Each of the genes was amplified using the genomic DNA of E. coli BL21(DE3) as a template, and also using the primers of SEQ ID NO: 13 and SEQ ID NO: 14, the primers of SEQ ID NO: 15 and SEQ ID NO: 16, and the primers of SEQ ID NO: 17 and SEQ ID NO: 18, respectively.
(50) At this time, the sequence of a ribosome binding site (RBS) was added to the 5′ end of the amplified hemL gene by the sequence of the ribosome binding site (RBS) contained in the primer of SEQ ID NO: 15 used in the amplification process. In addition, the hemA gene derived from BL21(DE3) was converted to the negative feedback resistance hemA (hemA.sup.fbr) by the nucleotide sequence encoding threonine and leucine, contained in the primer of SEQ ID NO: 17.
(51) TABLE-US-00002 TABLE 2 SEQ ID NOs Nucleotide sequences SEQ ID 5′-GGAATTCCAT ATGAAAATCA AAACTCGCTT NO: 13 C-3′ SEQ ID 5′-CCGCTCGAGT TACTGCTGAT TTTCGCGT-3′ NO: 14 SEQ ID 5′-CGAGCTCATA AAAGGAGGAA AATATATGAG NO: 15 TAAGTCTGAA AATCTTTACA G-3′ SEQ ID 5′-AAGGAAAAAA GCGGCCGCTC ACAACTTCGC NO: 16 AAACACC-3′ SEQ ID 5′-CATGCCATGG GTACCAAGAA GCTTTTAGCA NO: 17 CTCGGTATCA ACC-3′ SEQ ID 5′-CGCGGATCCC TACTCCAGCC CGAGGCT-3′ NO: 18
(52) The amplified gltX, hemA.sup.fbr and hemL gene sequences were cleaved with NdeI and XhoI, NcoI and BamHI, and SacI and NotI, respectively, and then sequentially inserted into a pRSFDuet-1 vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pRSF-05.
1-2: Comparison of ALA Production Between E. coli BL21(DE3) Strains that Overexpressed the C4 and C5 ALA Biosynthetic Genes
(53) To compare ALA production obtained when strains introduced with the C4 and C5 ALA biosynthetic pathways, respectively, were cultured without feeding precursors which are used by the respective pathways (glycine and succinate for the C4 ALA biosynthetic pathway, and glutamate for the C5 ALA biosynthetic pathway), each of the pRSF-C4 and pRSF-05 vectors constructed in Example 1-1 was introduced into an E. coli BL21(DE3) strain, thereby constructing an E. coli C4 strain and an E. coli C5 strain (Table 3). As a negative control, E. coli BL21(DE3) was used (Table 3).
(54) TABLE-US-00003 TABLE 3 Strain Description BL21(DE3) E. coli BL21(DE3) C4 E. coli BL21(DE3) harboring pRSF-C4 C5 E. coli BL21(DE3) harboring pRSF-C5
(55) Each of the strains shown in Table 3 was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, g/L yeast extract) supplemented with 25 μg/mL of kanamycin, and was then cultured at 37° C. and 220 rpm for 12 hours. To produce ALA using the strains, 1 mL of the pre-culture was transferred to 50 mL of the same fresh medium, and then cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(56) After the culture, samples were collected to analyze the production of ALA, and then the samples were pretreated and analyzed by a previously reported method (Burnham, Methods Enzymol. 17A, 195-204, 1970). As a result, it was shown that 0.02 g/L, 0.31 g/L and 1.74 g/L of ALA were produced in the BL21(DE3) strain (negative control), the C4 strain and the C5 strain, respectively, indicating that when the precursors are not fed, the production of ALA by the C5 ALA biosynthetic pathway is at least 5-fold higher than that by the C4 ALA biosynthetic pathway (
Example 2: Construction of Heme-Producing Strain Introduced with C5 ALA Biosynthetic Pathway
2-1: Selection of Overexpressing Target Gene for Heme Production
(57) To produce heme from the ALA produced via the ALA biosynthetic pathway, the enzymes expressed from hemB, hemC, hemD, hemE, hemF, hemG and hemH genes are required (
(58) (a) Confirmation of the Need for Overexpression of hemB and hemH Genes for Heme Production
(59) It was reported that when the hemB gene (SEQ ID NO: 19) and hemH gene (SEQ ID NO: 20) among the heme biosynthetic genes are overexpressed, the production of ALA used as a precursor in heme production decreases (Zhang et al., Sci. Rep. 5, 8584, 2015), and on the contrary, when the expression levels of the two genes are decreased, the metabolism of the metabolic pathway that produces heme from ALA decreases (Li et al., FETES Microbiol. Lett. 350, 209-215, 2014). Thus, it was concluded that overexpression of the hemB and hemH genes is necessary for effective heme overproduction.
(60) The vector pET-hemBH, which overexpresses the two genes and does not interfere with a plasmid overexpressing the C5 ALA biosynthetic pathway, was constructed through the following procedure.
(61) The primers of SEQ ID NOs: 21 and 22 and the primers of SEQ ID NOs: 23 and 24 were respectively used to amplify the hemB and hemH genes from the genomic DNA of the E. coli BL21(DE3) strain.
(62) TABLE-US-00004 TABLE 4 SEQ ID NOs: Nucleotide sequences SEQ ID 5′-GGAATTCCAT ATGACAGACT TAATCCAACG NO: 21 C-3′ SEQ ID 5′-CCGCTCGAGT TAACGCAGAA TCTTCTTCTC NO: 22 AG-3′ SEQ ID 5′-GGAATTCCAT ATGCGTCAGA CTAAAACCGG- NO: 23 3′ SEQ ID 5′-CCGCTCGAGT TAGCGATACG CGGCAAC-3′ NO: 24
(63) Each of the amplified sequences was cleaved with NdeI and XhoI, and then inserted into a pETDuet-1 vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pET-hemB and pET-hemH.
(64) The primers of SEQ ID NOs: 25 and 26 were used to amplify the hemB gene from the genomic DNA of the E. coli BL21(DE3) strain, and each of the amplified sequences was cleaved with NcoI and BamHI, and then inserted into a pET-hemH vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pET-hemBH.
(65) TABLE-US-00005 TABLE 5 SEQ ID NOs Nucleotide sequences SEQ ID 5′-CATGCCATGG GTACAGACTT AATCCAACGC-3′ NO: 25 SEQ ID 5′-CGCGGATCCT TAACGCAGAA TCTTCTTCTC NO: 26 AG-3′
(66) Each of the constructed pET-hemB, pET-hemH and pET-hemBH was introduced into a recombinant E. coli C5 strain, thereby constructing E. coli C5-B, C5-H and C5-BH strains (Table 6).
(67) TABLE-US-00006 TABLE 6 Strain Description C5-B E. coli BL21(DE3) harboring pRSF-C5 and pET-hemB C5-H E. coli BL21(DE3) harboring pRSF-C5 and pET-hemH C5-BH E. coli BL21(DE3) harboring pRSF-C5 and pET-hemBH
(68) In order to identify the profiles of ALA production, heme and heme biosynthetic intermediates (uroporphyrinogen III, coproporphyrinogen III, and protoporphyrin IX) in the constructed E. coli strains, each of the strains shown in Table 6 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin and 50 μg/mL of ampicillin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 50 mL of LB-Fe7.5 medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 7.5 mg/L FeSO.sub.47H.sub.2O) containing the same antibiotics, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(69) After the culture, samples were collected to analyze the production of ALA, and then the samples were pretreated and analyzed by a previously reported method (Burnham, Methods Enzymol. 17A, 195-204, 1970).
(70) In addition, in order to analyze the production of heme, 1 mL of a sample was collected from each culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(71) To confirm the heme biosynthetic intermediates, the same samples as used to analyze the production of heme were used, but a previously reported method using HPLC-MS was used (Pranawidjaja et al., J. Microbiol. Biotechnol. 25, 880-886, 2015; Bu et al., J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 783, 411-423, 2003).
(72) As a result, as expected, the C5-BH strain produced large amounts of the heme biosynthetic intermediates (uroporphyrinogen III and coproporphyrinogen III) than the other two strains (Table 7). In addition, the amount of ALA found was smaller in C5-BH than in C5-B and C5-H. However, free heme could not be detected in all the three strains.
(73) TABLE-US-00007 TABLE 7 Uroporphy- Copropor- Protopor- ALA rinogen III phyrinogen III phyrin IX Heme Strains (g l.sup.−1) (AU).sup.b (AU).sup.b (AU).sup.b (g l.sup.−1) C5-B 0.712 153 119 — — C5-H 1.409 — — — — C5-BH 0.726 787 158 — —
(74) (b) Confirmation of the Need for Overexpression of hemC, hemD, hemE, hemF and hemG Genes for Overproduction of Heme
(75) In order to select genes of which overexpression is necessary for overproduction of heme among the remaining five genes (hemC(SEQ ID NO: 27), hemD(SEQ ID NO: 28), hemE(SEQ ID NO: 29), hemF(SEQ ID NO: 30), hemG(SEQ ID NO: 31)) other than the hemB and hemH genes among the heme biosynthetic genes, each of the five genes was overexpressed in the C5-BH strain, and then the production of heme and the profiles of production of heme biosynthetic intermediates were analyzed. Plasmids pCDF-hemC, pCDF-hemD, pCDF-hemE, pCDF-hemF and pCDF-hemG respectively overexpressing the five genes were constructed through the following procedure.
(76) The primers of SEQ ID NOs: 32 and 33, the primers of SEQ ID NOs: 34 and 35, the primers of SEQ ID NOs: 36 and 37, the primers of SEQ ID NOs: 38 and 39, and the primers of SEQ ID NOs: 40 and 41 were respectively used to amplify the hemC, hemD, hemE, hemF, and hemG genes from the genomic DNA of the E. coli BL21(DE3) strain.
(77) TABLE-US-00008 TABLE 8 SEQ ID NOs Nucleotide sequences SEQ ID 5′-CATGCCATGG GTATGTTAGA CAATGTTTTA NO: 32 AGAATTGC-3′ SEQ ID 5′-CGCGGATCCT CATGCCGGAG CGTCTC-3′ NO: 33 SEQ ID 5′-CATGCCATGG GTATGAGTAT CCTGGTCACC NO: 34 CG-3′ SEQ ID 5′-CGCGGATCCT TATTGTAATG CCCGTAAAAG NO: 35 C-3′ SEQ ID 5′-CATGCCATGG GTATGACCGA ACTTAAAAAC NO: 36 GATC-3′ SEQ ID 5′-CCGGAATTCT TAGCGGTGAT ATTGTTCAGA NO: 37 C-3′ SEQ ID 5′-CATGCCATGG GTATGAAACC CGACGCACAC-3′ NO: 38 SEQ ID 5′-CGCGGATCCT TACACCCAAT CCCTGACCT-3′ NO: 39 SEQ ID 5′-CATGCCATGG GTGTGAAAAC ATTAATTCTT NO: 40 TTCTCAAC-3′ SEQ ID 5′-CGAGCTCTTA TTTCAGCGTC GGTTTGTC-3′ NO: 41
(78) Each of the amplified genes was cleaved with NcoI and BamHI, NcoI and BamHI, NcoI and EcoRI, NcoI and BamHI, and NcoI and SacI, and then inserted into a pCDFDuet-1 vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pCDF-hemC, pCDF-hemD, pCDF-hemE, pCDF-hemF, and pCDF-hemG, respectively.
(79) Each of the constructed pCDF-hemC, pCDF-hemD, pCDF-hemE, pCDF-hemF, and pCDF-hemG was introduced into a recombinant E. coli C5-BH strain, thereby constructing E. coli C5-BCH, C5-BDH, C5-BEH, C5-BFH, and C5-BGH strains (Table 9).
(80) TABLE-US-00009 TABLE 9 Strain Description C5-BCH E. coli BL21(DE3) harboring pRSF-C5, pET-hemBH, and pCDF-hemC C5-BDH E. coli BL21(DE3) harboring pRSF-C5, pET-hemBH, and pCDF-hemD C5-BEH E. coli BL21(DE3) harboring pRSF-C5, pET-hemBH, and pCDF-hemE C5-BFH E. coli BL21(DE3) harboring pRSF-C5, pET-hemBH, and pCDF-hemF C5-BGH E. coli BL21(DE3) harboring pRSF-C5, pET-hemBH, and pCDF-hemG
(81) In order to identify the profiles of production of ALA, heme and heme biosynthetic intermediates (uroporphyrinogen III, coproporphyrinogen III, and protoporphyrin IX) in the constructed E. coli strains, each of the strains shown in Table 9 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin and 50 μg/mL of ampicillin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 50 mL of LB-Fe7.5 medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 7.5 mg/L FeSO.sub.47H.sub.2O) containing the same antibiotics, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(82) After the culture, samples were collected to analyze the production of ALA, and then the samples were pretreated and analyzed by a previously reported method (Burnham, Methods Enzymol. 17A, 195-204, 1970).
(83) In addition, in order to analyze the production of heme, 1 mL of a sample was collected from each culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(84) To confirm the heme biosynthetic intermediates, the same samples as used to analyze the production of heme were used, but a previously reported method using HPLC-MS was used (Pranawidjaja et al., J. Microbiol. Biotechnol. 25, 880-886, 2015; Bu et al., J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 783, 411-423, 2003).
(85) As a result, although heme was not detected in all the five strains (
(86) TABLE-US-00010 TABLE 10 Uroporphy- Copropor- Protopor- ALA rinogen III phyrinogen III phyrin IX Heme Strains (g l.sup.−1) (AU).sup.b (AU).sup.b (AU).sup.b (g l.sup.−1) C5-BCH 0.553 1259 164 — — C5-BDH 0.459 1460 429 89 — C5-BEH 0.494 693 549 — — C5-BFH 0.462 680 525 — — C5-BGH 0.463 717 134 — —
(87) However, the C5-BGH strain did not significantly differ from the C5-BH strain (Table 7) in terms of the production of ALA and heme biosynthetic intermediates (Table 10). In order to confirm again whether overexpression of hemG is essential for heme overproduction, the strain HEME1 overexpressing the hemC, hemD, hemE and hemF genes was constructed based on the C5-BH strain, and the strain HEME2 overexpressing all the hemC, hemD, hemE, hemF and hemG genes was constructed based on the C5-BH strain through the following procedures.
(88) A plasmid pCDF-hemCDEF overexpressing the hemC, hemD, hemE and hemF genes was constructed through the following procedure.
(89) The primers of SEQ ID NOs: 42 and 43, the primers of SEQ ID NOs: 44 and 45, the primers of SEQ ID NOs: 46 and 47, and the primers of SEQ ID NOs: 48 and 49 were respectively used to amplify the hemC, hemD, hemE and hemF genes from the genomic DNA of the E. coli BL21(DE3) strain.
(90) At this time, the sequence of a ribosome binding site (RBS) was added to the 5′ ends of the amplified hemD and hemF genes by the sequence of the ribosome binding site (RBS) contained in the primers of SEQ ID NOs: 44 and 48 used in the amplification process.
(91) TABLE-US-00011 TABLE 11 SEQ ID NOs Nucleotide sequences SEQ ID 5′-GGGAATTCCA TATGTTAGAC AATGTTTTAA NO: 42 GAATTGC-3′ SEQ ID 5′-GAAGATCTTC ATGCCGGAGC GTCTC-3′ NO: 43 SEQ ID 5′-GAAGATCTAT AAAAGGAGGA AAATATATGA NO: 44 GTATCCTGGT CACCCG-3′ SEQ ID 5′-CCGCTCGAGT TATTGTAATG CCCGTAAAAG NO: 45 C-3′ SEQ ID 5′-CATGCCATGG GTACCGAACT TAAAAACGAT NO: 46 C-3′ SEQ ID 5′-CGAGCTCTTA GCGGTGATAT TGTTCAGAC-3′ NO: 47 SEQ ID 5′-CGAGCTCATA AAAGGAGGAA AATATATGAA NO: 48 ACCCGACGCA CAC-3′ SEQ ID 5′-AAAACTGCAG TTACACCCAA TCCCTGACCT-3′ NO: 49
(92) Each of the amplified sequences was cleaved with NdeI and BglII, BglII and XhoI, NcoI and SacI, and SacI and PstI, and then sequentially inserted into a pCDFDuet-1 vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pCDF-hemCDEF.
(93) A plasmid pCDF-hemCDEFG overexpressing the hemC, hemD, hemE, hemF, and hemG genes was constructed through the following procedure.
(94) The primers of SEQ ID NOs: 50 and 51 were respectively used to amplify the hemG gene from the genomic DNA of the E. coli BL21(DE3) strain. At this time, the sequence of a ribosome binding site (RBS) was added to the 5′ end of the amplified hemG gene by the sequence of the ribosome binding site (RBS) contained in the primer of SEQ ID NO: 50 used in the amplification process.
(95) TABLE-US-00012 TABLE 12 SEQ ID NOs Nucleotide sequences SEQ ID 5′-AAAACTGCAG ATAAAAGGAG GAAAATATGT NO: 50 GAAAACATTA ATTCTTTTCT CAAC-3′ SEQ ID 5′-AAGGAAAAAA GCGGCCGCTT ATTTCAGCGT NO: 51 CGGTTTGTC-3′
(96) Each of the amplified sequences was cleaved with PstI and NotI, and then inserted into a pCDF-hemCDEF vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pCDF-hemCDEFG.
(97) Each of the constructed pCDF-hemCDEF and pCDF-hemCDEFG was introduced into a recombinant E. coli C5-BH strain, thereby constructing E. coli HEME1 and HEME2 strains (
(98) TABLE-US-00013 TABLE 13 Strain Description HEME1 E. coli BL21(DE3) harboring pRSF-C5, pET-hemBH, and pCDF-hemCDEF HEME2 E. coli BL21(DE3) harboring pRSF-C5, pET-hemBH, and pCDF-hemCDEFG
(99) In order to identify the profiles of production of ALA and heme in the constructed E. coli strains, each of the strains shown in Table 13 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, and 100 μg/mL streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the culture medium in which the cultured cells were growing was used to inoculate 50 mL of LB-Fe7.5 medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 7.5 mg/L FeSO.sub.47H.sub.2O) containing the same antibiotics, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(100) After the culture, samples were collected to analyze the production of ALA, and then the samples were pretreated and analyzed.
(101) In addition, in order to analyze the production of heme, 1 mL of a sample was collected from each culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured using HPLC.
(102) As a result, 0.14 mg/L and 0.53 mg/L of heme were detected in the HEME1 and HEME2 strains, respectively (
2-2: Regulation of Overexpression Levels of Heme Biosynthetic Genes for Increased Production of Heme
(103) Since the production of ALA (308 mg/L) in the HEME2 strain constructed in Example 2-1 was significantly higher than the production of heme (0.53 mg/L), it was considered that the metabolic flux of the ALA biosynthetic pathway is excessive. Under the assumption that the total amount of proteins that can be overexpressed in a strain is limited, the production of heme was increased by enhancing the expression of metabolic proteins responsible for production of heme from ALA and reducing the expression of metabolic proteins responsible for the relatively excessive ALA-producing pathway, through the following procedures.
(104) (a) Construction of plasmid pCDF-hemAL
(105) Based on the plasmid pCDFDuet-1 having a lower copy number than the plasmid pRSFDuet-1 (Novagen, USA), the genes of the C5 ALA biosynthetic pathway were overexpressed, so that the expression levels of the ALA biosynthetic pathway could be reduced compared to when pRSF-C5 was used, and as a result, the metabolism of the corresponding pathway could also be reduced. In addition, the gene gltX encoding glutamyl tRNA synthetase (GluRS) that converts glutamate to glutamyl-tRNA in the C5 ALA biosynthetic pathway was not overexpressed so that the overexpression levels of other proteins could be increased. Based on pCDFDuet-1, the plasmid pCDF-hemAL overexpressing the C5 ALA biosynthetic pathway was constructed through the following procedures.
(106) The primers of SEQ ID NOs: 15 and 16 and the primers of SEQ ID NOs: 17 and 18 were respectively used to amplify the hemL and negative feedback-resistant hemA (hemA.sup.fbr) genes from the genomic DNA of the E. coli BL21(DE3) strain.
(107) At this time, the sequence of a ribosome binding site (RBS) was added to the 5′ end of the amplified hemL gene by the sequence of the ribosome binding site (RBS) contained in the primer of SEQ ID NO: 15 used in the amplification process. In addition, the hemA gene derived from BL21(DE3) was converted to the negative feedback-resistant hemA (hemA.sup.fbr) by inserting the nucleotide sequence encoding threonine and leucine, contained in the primer of SEQ ID NO: 17.
(108) The amplified DNA fragments were cleaved with NcoI and BamHI, and SacI and NotI, respectively, and then sequentially inserted into plasmid pCDFDuet-1 (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pCDF-hemAL.
(109) (b) Construction of plasmid pRSF-hemBCD
(110) Based on the plasmid pRSFDuet-1 having a higher copy number than the plasmid pETDuet-1, the hemB, hemC and hemD genes involved in the upstream of the metabolic pathway that produces heme from ALA were overexpressed so that the flux of the metabolic pathway that produces heme from ALA would be increased. Based on pRSFDuet-1, the plasmid pRSF-hemBCD overexpressing the hemB, hemC, hemD genes was constructed through the following procedures.
(111) The primers of SEQ ID NOs: 25 and 26, the primers of SEQ ID NOs: 42 and 43, and the primers of SEQ ID NOs: 44 and 45 were respectively used to amplify the hemB, hemC, and hemD genes from the genomic DNA of the E. coli BL21(DE3) strain.
(112) At this time, the sequence of a ribosome binding site (RBS) was added to the 5′ end of the amplified hemD gene by the sequence of the ribosome binding site (RBS) contained in the primer of SEQ ID NO: 44 used in the amplification process.
(113) The amplified DNA fragments were cleaved with NcoI and BamHI, NdeI and BglII, and BglII and XhoI, respectively, and then sequentially inserted into a pRSFDuet-1 vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pRSF-hemBCD.
(114) (c) Construction of Plasmid pET-hemEFGH
(115) Based on the plasmid pETDuet-1 having a higher copy number than the plasmid pCDFDuet-1, the hemE, hemF, hemG and hemH genes involved in the downstream of the metabolic pathway that produces heme from ALA were overexpressed so that the flux of the metabolic pathway that produces heme from ALA would be increased. Based on pETDuet-1, the plasmid pET-hemEFGH overexpressing the hemE, hemF, hemG and hemH genes was constructed through the following procedures.
(116) The primers of SEQ ID NOs: 46 and 47, the primers of SEQ ID NOs: 48 and 49, the primers of SEQ ID NOs: 50 and 51, and the primers of SEQ ID NOs: 23 and 24 were respectively used to amplify the hemE, hemF, hemG and hemH genes from the genomic DNA of the E. coli BL21(DE3) strain.
(117) At this time, the sequence of a ribosome binding site (RBS) was added to the 5′ ends of the amplified hemF and hemG genes by the sequence of the ribosome binding site (RBS) contained in the primers of SEQ ID NOs: 48 and 50 used in the amplification process.
(118) The amplified sequences were cleaved with NcoI and SacI, SacI and PstI, PstI and NotI, and NdeI and XhoI, respectively, and then sequentially inserted into a pETDuet-1 vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pET-hemEFGH.
(119) (d) Construction of Recombinant E. coli HEME3
(120) The constructed pCDF-hemAL, pRSF-hemBCD and pET-hemEFGH plasmids were introduced together into the E. coli BL21(DE3) strain, thereby constructing an E. coli HEME3 strain (
(121) TABLE-US-00014 TABLE 14 Strain Description HEME3 E. coli BL21(DE3) harboring pCDF-hemAL, pRSF-hemBCD, and pET-hemEFGH
(122) In order to identify the profiles of ALA and heme production in the constructed E. coli strains, each of the strains shown in Table 14 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, and 100 μg/mL streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 50 mL of LB-Fe7.5 medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 7.5 mg/L FeSO.sub.47H.sub.2O) containing the same antibiotics, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(123) After the culture, samples were collected to analyze the production of ALA, and then the samples were pretreated and analyzed by a previously reported method (Burnham, Methods Enzymol. 17A, 195-204, 1970).
(124) In addition, in order to analyze the production of heme, 1 mL of a sample was collected from each culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(125) As a result, the production of heme in the HEME3 strain was increased to 3.79 mg/L, compared to the HEME2 strain (0.53 mg/L) (
Example 3: Deletion of Competitive Metabolic Pathways for Increased Production of Heme
(126) For increased production of heme, metabolic pathways competing with the heme biosynthetic pathway can be deleted. First, to increase the metabolic flux from glucose to glutamate, metabolites of glycolysis that serve as precursors of C5 ALA biosynthesis can be prevented from being converted to by-products such as acetate or lactate. In addition, the produced heme can be prevented from being degraded, thereby increasing the production of heme.
3-1: Deletion of/dhA and Pta Genes for Increased Production of Heme
(127) In order to increase the yield and metabolism for conversion of glucose to glutamate in the strain of the present invention that uses the C5 ALA biosynthetic pathway, pyruvate and acetyl-CoA, which are metabolites of glycolysis, need to be prevented from being converted to lactate and acetate, respectively. Based on a previous report indicating that the production of glutamate can be increased by deleting the ldhA gene involved in the conversion of pyruvate to lactate and the pta gene involved in the conversion of acetyl-CoA to acetate (Vuoristo et al., AMB Express 5, 61, 2015), the ldhA and pta genes were deleted from HEME3 through the following procedures.
(128) (a) Construction of DNA Fragments for Gene Deletion
(129) To delete the ldhA and pta genes using a previously reported A Red recombineering technique (Datsenko et al., Proc. Natl. Acad. Sci. USA 97, 6640-6645, 2000), DNA fragments required for gene deletion were constructed. A DNA fragment (ΔldhA::cat) to be used to delete the ldhA gene from the plasmid pECmulox (Kim et al., FEMS Microbiol. Lett. 278, 78-85, 2008) and a DNA fragment (Δpta::cat) to be used to delete the pta gene were amplified using the primers of SEQ ID NOs: 52 and 53 and the primers of SEQ ID NOs: 54 and 55, respectively.
(130) TABLE-US-00015 TABLE 15 SEQ ID NOs Nucleotides SEQ ID 5′-TATTTTTAGT AGCTTAAATG TGATTCAACA NO: 52 TCACTGGAGA AAGTCTTATG TAGGTGACAC TATAGAACGC G-3′ SEQ ID 5′-CTCCCCTGGG TTGCAGGGGA GCGGCAAGAT NO: 53 TAAACCAGTT CGTTCGGGCA TAGTGGATCT GATGGGTACC-3′ SEQ ID 5′-GCTGTTTTGT AACCCGCCAA ATCGGCGGTA NO: 54 ACGAAAGAGG ATAAACCGTG TAGGTGACAC TATAGAACGC G-3′ SEQ ID 5′-GCAGCGCAAA GCTGCGGATG ATGACGAGAT NO: 55 TACTGCTGCT GTGCAGACTG TAGTGGATCT GATGGGTACC-3′
(131) (b) Deletion of ldhA Gene from BL21(DE3)
(132) In order to delete the ldhA gene using the above-constructed DNA fragment, the plasmid pKD46 (Datsenko et al., Proc. Natl. Acad. Sci. USA 97, 6640-6645, 2000) was introduced into the BL21(DE3) strain which was then inoculated into 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 50 μg/mL ampicillin, and was cultured overnight at 30° C. and 200 rpm. The cultured cells in 1 mL of the culture broth were used to inoculate 100 mL of LB medium supplemented with 50 μg/mL of ampicillin and 1 mM of arabinose, and were then cultured at 30° C. and 200 rpm until the OD.sub.600 reached about 0.6. The cultured cells were harvested, washed twice with 10% glycerol cooled to 0° C., and were then resuspended in 150 μL of the same aqueous solution. Next, the constructed DNA fragment (ΔldhA::cat) for deletion of the ldhA gene was introduced into the cells by electroporation, and then a colony introduced with chloramphenicol resistance gene (cat) while lacking the ldhA gene was selected by culture on LB-agar medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 15 g/L agar) containing 8.5 μg/mL of chloramphenicol at 37° C.
(133) After removal of the plasmid pKD46 from the selected colonies during the culture at 37° C. for colony selection was confirmed, the plasmid pJW168 (Wild et al., Gene 223, 55-66, 1998) was introduced again into the colony which was then cultured on LB-agar medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 15 g/L agar) containing 50 μg/mL of ampicillin and 1 mM IPTG at 30° C., thereby removing the chloramphenicol resistance gene (cat). Deletion of the ldhA gene from the constructed strain BL21(DE3) ΔldhA (Table 16) was confirmed by sequencing.
(134) TABLE-US-00016 TABLE 16 Strain Description Bl21(DE3) ΔldhA E. coli BL21(DE3) ΔldhA
(135) (c) Deletion of Pta Gene from BL21(DE3) ΔldhA
(136) In order to delete the pta gene using the above-constructed DNA fragment, the plasmid pKD46 (Datsenko et al., Proc. Natl. Acad. Sci. USA 97, 6640-6645, 2000) was introduced into the BL21(DE3) strain which was then used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 50 μg/mL ampicillin, and was cultured overnight at 30° C. and 200 rpm. The cultured cells in 1 mL of the culture broth were transferred to 100 mL of LB medium supplemented with 50 μg/mL of ampicillin and 1 mM of arabinose, and were then cultured at 30° C. and 200 rpm until the OD.sub.600 reached about 0.6. The cultured cells were harvested, washed twice with 10% glycerol cooled to 0° C., and were then resuspended in 150 μL of the same aqueous solution. Next, the constructed DNA fragment (Δpta::cat) for deletion of the pta gene was introduced into the cells by electroporation, and then a colony introduced with chloramphenicol resistance gene (cat) while lacking the pta gene was selected by culture on LB-agar medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 15 g/L agar) containing 8.5 μg/mL of chloramphenicol at 37° C.
(137) After removal of the plasmid pKD46 from the selected colonies during the culture at 37° C. for colony selection was confirmed, the plasmid pJW168 (Wild et al., Gene 223, 55-66, 1998) was introduced again into the colony which was then cultured on LB-agar medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 15 g/L agar) containing 50 μg/mL of ampicillin and 1 mM IPTG at 30° C., thereby removing the chloramphenicol resistance gene (cat). Deletion of the pta gene from the constructed strain BL21(DE3) Δpta ΔldhA was confirmed by sequencing.
(138) TABLE-US-00017 TABLE 17 Strain Description Bl21(DE3) Δpta ΔldhA E. coli BL21(DE3) Δpta ΔldhA
(139) (d) Construction of Recombinant E. coli HEME5
(140) The pCDF-hemAL, pRSF-hemBCD and pET-hemEFGH plasmids constructed in Example 2-2 were introduced together into the strain BL21(DE3) Δpta ΔldhA shown in Table 17, thereby constructing an E. coli HEME5 strain (Table 18).
(141) TABLE-US-00018 TABLE 18 Strain Description HEME5 E. coli BL21(DE3) Δpta ΔldhA harboring pCDF- hemAL, pRSF-hemBCD, and pET-hemEFGH
(142) In order to identify the profiles of ALA and heme production in the constructed E. coli strain, the strain shown in Table 18 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, and 100 μg/mL streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 50 mL of LB-Fe7.5 medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 7.5 mg/L FeSO.sub.47H.sub.2O) containing the same antibiotics, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(143) After the culture, samples were collected to analyze the production of ALA, and then the samples were pretreated and analyzed by a previously reported method (Burnham, Methods Enzymol. 17A, 195-204, 1970).
(144) In addition, in order to analyze the production of heme, 1 mL of a sample was collected from each culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(145) As a result, the production of heme in the HEME5 strain was increased to 4.17 mg/L, compared to the HEME3 strain (3.79 mg/L) (
3-2: Deletion of yfeX Gene for Increased Production of Heme
(146) A previous study reported that the protein expressed from the yfeX gene is heme dechelatase that removes Fe.sup.2+ ions from heme (Letoffe et al., Proc. Natl. Acad. Sci. USA 106, 11719-11724, 2009), but another study reported that the expression product of the yfeX gene is not heme dechelatase, but peroxidase that converts porphyrinogen to porphyrin (Dailey et al., mBio 2, e00248-00211, 2011). However, still another study reported that overexpression of the yfeX gene disrupts the homeostasis of heme (Turlin et al., Microbiology Open 3, 849-859, 2014). Although the exact function of the yfeX gene has not yet been found, it was considered that deletion of the yfeX gene can increase the production of heme. Thus, the effect of deleting the yfeX gene on the production of heme was examined through the following procedures.
(147) (a) Construction of DNA Fragments for Gene Deletion
(148) To delete the yfeX gene using a previously reported λ Red recombineering technique (Datsenko et al., Proc. Natl. Acad. Sci. USA 97, 6640-6645, 2000), DNA fragments required for gene deletion were constructed. A DNA fragment (ΔyfeX::cat) to be used to delete the yfeX gene was amplified from the plasmid pECmulox (Kim et al., FEMS Microbiol. Lett. 278, 78-85, 2008) using the primers of SEQ ID NOs: 56 and 57.
(149) TABLE-US-00019 TABLE 19 SEQ ID NOs Nucleotide sequences SEQ ID 5′-TCAACAATGC CACGGATTGC GTGGCATTCT NO: 56 TATTTTCAGG AGGAACAATG TAGGTGACAC TATAGAACGC G-3′ SEQ ID 5′-CTGGCCTTTA ATCAATGAAT CAGAAACGCT NO: 57 TACAGCGCCA TCAACTTGTC TAGTGGATCT GATGGGTACC-3′
(150) (b) Deletion of yfeX Gene from BL21(DE3)
(151) In order to delete the yfeX gene using the above-constructed DNA fragment, the plasmid pKD46 (Datsenko et al., Proc. Natl. Acad. Sci. USA 97, 6640-6645, 2000) was introduced into the BL21(DE3) strain which was then used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 50 μg/mL ampicillin, and was cultured overnight at 30° C. and 200 rpm. The cultured cells in 1 mL of the culture broth were transferred to 100 mL of LB medium supplemented with 50 μg/mL of ampicillin and 1 mM of arabinose, and were then cultured at 30° C. and 200 rpm until the OD.sub.600 reached about 0.6. The cultured cells were harvested, washed twice with 10% glycerol cooled to 0° C., and were then resuspended in 150 μL of the same aqueous solution. Next, the constructed DNA fragment (ΔyfeX::cat) for deletion of the yfeX gene was introduced into the cells by electroporation, and then a colony introduced with chloramphenicol resistance gene (cat) while lacking the yfeX gene was selected by culture on LB-agar medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 15 g/L agar) containing 8.5 μg/mL of chloramphenicol at 37° C.
(152) After removal of the plasmid pKD46 from the selected colonies during culture at 37° C. for colony selection was confirmed, the plasmid pJW168 (Wild et al., Gene 223, 55-66, 1998) was introduced again into the colony which was then cultured on LB-agar medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 15 g/L agar) containing 50 μg/mL of ampicillin and 1 mM IPTG at 30° C., thereby removing the chloramphenicol resistance gene (cat). Deletion of the yfeX gene from the constructed strain BL21(DE3) ΔyfeX (table 20) was confirmed by sequencing.
(153) TABLE-US-00020 TABLE 20 Strain Description Bl21(DE3) ΔyfeX E. coli BL21(DE3) ΔyfeX
(154) (c) Construction of Recombinant E. coli HEME4
(155) The pCDF-hemAL, pRSF-hemBCD and pET-hemEFGH plasmids constructed in Example 2-2 were introduced together into the strain BL21(DE3) ΔyfeX shown in Table 20, thereby constructing an E. coli HEME4 strain (Table 21).
(156) TABLE-US-00021 TABLE 21 Strain Description HEME4 E. coli BL21(DE3) ΔyfeX harboring pCDF- hemAL, pRSF-hemBCD, and pET-hemEFGH
(157) In order to identify the profiles of ALA and heme production in the constructed E. coli strains, the strain shown in Table 21 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, and 100 μg/mL streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 50 mL of LB-Fe7.5 medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 7.5 mg/L FeSO.sub.47H.sub.2O) containing the same antibiotics, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(158) After the culture, samples were collected to analyze the production of ALA, and then the samples were pretreated and analyzed by a previously reported method (Burnham, Methods Enzymol. 17A, 195-204, 1970).
(159) In addition, in order to analyze the production of heme, 1 mL of a sample was collected from each culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(160) As a result, the production of heme in the HEME4 strain was increased to 6.21 mg/L, compared to the HEME3 strain (3.79 mg/L) (
3-3: Deletion of Pta, ldhA and yfeX Genes for Increased Production of Heme
(161) As described in Examples 3-1 and 3-2, the production of heme in each of the HEME5 strain (4.17 mg/mL heme) lacking the pta and ldhA genes and the HEME4 strain (6.21 mg/mL heme) lacking the yfeX gene was increased compared to that in the HEME3 strain (3.79 mg/L). Thus, in order to examine whether the production of heme can further be increased when the gene deletion strategies of Examples 3-1 and Example 3-2 are used together, a strain lacking all the three genes was constructed and analyzed through the following procedures.
(162) (a) Construction of DNA Fragments for Gene Deletion
(163) To delete the yfeX gene using a previously reported A Red recombineering technique (Datsenko et al., Proc. Natl. Acad. Sci. USA 97, 6640-6645, 2000), DNA fragments required for gene deletion were constructed. A DNA fragment (ΔyfeX::cat) to be used to delete the yfeX gene was amplified from the plasmid pECmulox (Kim et al., FEMS Microbiol. Lett. 278, 78-85, 2008) using the primers of SEQ ID NOs: 56 and 57.
(164) (b) Deletion of yfeX Gene from BL21(DE3) ΔPL
(165) In order to delete the yfeX gene using the above-constructed DNA fragment, the plasmid pKD46 (Datsenko et al., Proc. Natl. Acad. Sci. USA 97, 6640-6645, 2000) was introduced into the BL21(DE3) APL strain which was then used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 50 μg/mL ampicillin, and was cultured overnight at 30° C. and 200 rpm. The cultured cells in 1 mL of the culture broth were transferred to 100 mL of LB medium supplemented with 50 μg/mL of ampicillin and 1 mM of arabinose, and were then cultured at 30° C. and 200 rpm until the OD.sub.600 reached about 0.6. The cultured cells were harvested, washed twice with 10% glycerol cooled to 0° C., and were then resuspended in 150 μL of the same aqueous solution. Next, the constructed DNA fragment (ΔyfeX::cat) for deletion of the yfeX gene was introduced into the cells by electroporation, and then a colony introduced with chloramphenicol resistance gene (cat) while lacking the yfeX gene was selected by culture on LB-agar medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 15 g/L agar) containing 8.5 μg/mL of chloramphenicol at 37° C.
(166) After removal of the plasmid pKD46 from the selected colonies during the culture at 37° C. for colony selection was confirmed, the plasmid pJW168 (Wild et al., Gene 223, 55-66, 1998) was introduced again into the colony which was then cultured on LB-agar medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 15 g/L agar) containing 50 μg/mL of ampicillin and 1 mM IPTG at 30° C., thereby removing the chloramphenicol resistance gene (cat).
(167) Deletion of the yfeX gene from the constructed strain BL21(DE3) Δpta ΔldhA ΔyfeX (table 22) was confirmed by sequencing.
(168) TABLE-US-00022 TABLE 22 Strain Description Bl21(DE3) Δpta ΔldhA ΔyfeX E. coli BL21(DE3) Δpta ΔldhA ΔyfeX
(169) (c) Construction of Recombinant E. coli HEME6
(170) The pCDF-hemAL, pRSF-hemBCD and pET-hemEFGH plasmids constructed in Example 2-2 were introduced together into the strain BL21(DE3) Δpta ΔldhA ΔyfeX shown in Table 22, thereby constructing an E. coli HEME6 strain (
(171) TABLE-US-00023 TABLE 23 Strain Description HEME6 E. coli BL21(DE3) Δpta ΔldhA ΔyfeX harboring pCDF-hemAL, pRSF-hemBCD, and pET-hemEFGH
(172) In order to identify the profiles of production of ALA and heme in the constructed E. coli strains, the strain shown in Table 23 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, and 100 μg/mL streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 50 mL of LB-Fe7.5 medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract, 7.5 mg/L FeSO.sub.47H.sub.2O) containing the same antibiotics, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(173) After the culture, samples were collected to analyze the production of ALA, and then the samples were pretreated and analyzed by a previously reported method (Burnham, Methods Enzymol. 17A, 195-204, 1970).
(174) In addition, in order to analyze the production of heme, 1 mL of a sample was collected from each culture broth and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(175) As a result, the production of heme in the HEME6 strain was increased to 7.13 mg/L, compared to the HEME3 strain (3.79 mg/L), HEME5 strain (4.17 mg/mL) and HEME4 strain (6.21 mg/mL) (
Example 4: Culture Condition Optimization for Increased Production of Heme
4-1: Optimization of Heme-Producing Medium: Selection of Basal Medium Composition
(176) To further increase the production of heme in the HEME6 strain constructed in Example 3, the composition of a medium which is used in culture of the strain was optimized. The HEME6 strain was cultured in each of LB-Fe7.5, TB-Fe7.5 and MR-Fe7.5 media obtained by adding 7.5 mg/L of FeSO.sub.47H.sub.2O to LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract), TB medium (16.43 g/L K.sub.2HPO.sub.4.3H.sub.2O, 2.31 g/L, KH.sub.2PO.sub.4, 4 g/L glycerol, 12 g/L tryptone, 24 g/L yeast extract) and MR-Fe0 medium (6.67 g/L KH.sub.2PO.sub.4, 4 g/L (NH.sub.4).sub.2HPO.sub.4, 0.8 g/L MgSO.sub.47H2O, 0.8 g/L citric acid, 5 g/L yeast extract, 5 mL/L trace metal solution, pH7.0), respectively, and the production of heme in each of the media was measured. The composition of trace metal solution I is as follows: 0.5 M HCl, 2 g/L CaCl.sub.2, 2.2 g/L ZnSO.sub.47H.sub.2O, 0.5 g/L MnSO.sub.44H.sub.2O, 1 g/L CuSO.sub.45H.sub.2O, 0.1 g/L (NH.sub.4).sub.6Mo.sub.7O.sub.244H.sub.2O, and 0.02 g/L Na.sub.2B.sub.4O.sub.710H.sub.2O.
(177) In order to identify the profiles of production of heme in the HEME6 strain cultured in the medium, the strain shown in Table 23 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, and 100 μg/mL streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 50 mL of fresh six media supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin and 100 μg/mL streptomycin, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(178) In order to analyze the production of heme, 1 mL of a sample was collected from each culture broth and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(179) As a result, it was confirmed that when the HEME6 strain was cultured in the MR-Fe7.5 medium, the largest amount of heme (7.45 mg/L) was produced (
4-2: Optimization of Heme-Producing Medium: Selection of Iron Ion Concentration
(180) Based on the fact that the production of heme in the MR-Fe7.5 medium selected in Example 4-1 significantly differs from the production of heme in the MR-Fe0 medium, whether the concentration of iron ions has a great effect on the production of heme was examined. Thus, the iron ion concentration was optimized to further increase the production of heme in the HEME6 strain. Based on the MR-Fe0 medium of Example 4-1, 0, 10, 20, 40, 60 and 100 mg/L of FeSO.sub.47H.sub.2O were added to the medium, thereby preparing MR-Fe0, MR-Fe10, MR-Fe20, MR-Fe40, MR-FE60 and MR-Fe100 media, respectively.
(181) In order to identify the profiles of production of heme in the HEME6 strain cultured in the medium, the strain shown in Table 23 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, and 100 μg/mL streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 50 mL of fresh six media supplemented with μg/mL of kanamycin, 50 μg/mL of ampicillin and 100 μg/mL streptomycin, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 30° C. and 200 rpm for 48 hours.
(182) In order to analyze the production of heme, 1 mL of a sample was collected from each culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(183) As a result, it was confirmed that when the medium contains 20 mg/L FeSO.sub.47H.sub.2O (MR-Fe20 medium), the largest amount of heme was produced (
4-3: Culture Temperature Optimization
(184) To select the optimum culture temperature to be used to produce heme by the HEME6 strain, the strain was cultured through the following procedure.
(185) The strain shown in Table 23 above was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) containing 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, and 100 μg/mL streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 50 mL of fresh MR-Fe20 medium supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin and 100 μg/mL streptomycin, and then the cells were cultured in a 250-mL Erlenmeyer flask at 37° C. and 200 rpm until the OD.sub.600 reached about 0.6. Next, 1 mM IPTG was added to the culture medium, and then the cells were cultured at 24° C., 30° C. and 37° C. and 200 rpm for 72 hours, and sampling was performed at 6-hour intervals for the first 24 hours and at 12-hour intervals after the first 24 hours.
(186) In order to analyze the production of heme, 1 mL of a sample was collected from the culture broth and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(187) As a result, it was confirmed that when the strain was cultured in the presence of 1 mM IPTG at 30° C. for 60 hours, the largest amount of heme (7.78 g/L) was produced (
Example 5: Extracellular Free Heme Production by Batch Fermentation and Fed-Batch Fermentation
5-1: Extracellular Free Heme Production by Batch Fermentation
(188) An example in which fermentation was performed using a fermenter for heme production has not been reported.
(189) Thus, based on the culture conditions optimized in Example 4, batch fermentation was performed using the HEME6 strain.
(190) The strain shown in Table 23 was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin and 100 μg/mL of streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 200 mL of fresh MR-Fe20 medium supplemented with 25 μg/mL of kanamycin, 50 of μg/mL ampicillin and 100 μg/mL of streptomycin, and then the cells were cultured in a 500-mL Erlenmeyer flask at 37° C. and 200 rpm for 12 hours. Next, the culture was transferred to a 6.6-L Bioflo3000 fermenter (New Brunswick Scientific Co., Edison, N.J.) containing 1.8 L of MR-Fe20 medium (pH7.0; air-saturated with stirring at 200 rpm and supply of 2 L/min of air at 30° C.) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, 100 μg/mL of streptomycin and 20 g/L of glucose. During fermentation, the temperature was maintained at 30° C., and the pH was maintained at 7.0 by 50% NH.sub.4OH. In addition, the dissolved oxygen (DO) level was maintained at 40% of the initial DO level by increasing the agitation speed up to 1000 rpm. When the OD.sub.600 reached 5, 1 mM IPTG was added to the cells, and for analysis of heme production, sampling was performed at 8-hour intervals.
(191) In order to analyze the production of heme, 1 mL of a sample was collected from the culture broth and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared medium and lysed cell samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(192) As a result, the largest amount of heme (a total of 14.24 mg/L) was produced at 56 hours, and 1.18 mg/L (8.29%) of the produced heme was detected in the medium. The overall productivity for 56 hours was 0.25 mg/L/h (
5-2: Extracellular Free Heme Production by Fed-Batch Fermentation
(193) Based on the batch fermentation results obtained in Example 5-1, fed-batch fermentation was performed to produce a larger amount of heme.
(194) The strain shown in Table 23 was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin and 100 μg/mL of streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 200 mL of fresh MR-Fe20 medium supplemented with 25 μg/mL of kanamycin, 50 of μg/mL ampicillin and 100 μg/mL of streptomycin, and then the cells were cultured in a 500-mL Erlenmeyer flask at 37° C. and 200 rpm for 12 hours. Next, the culture was transferred to a 6.6-L Bioflo3000 fermenter (New Brunswick Scientific Co., Edison, N.J.) containing 1.8 L of MR-Fe20 medium (pH7.0; air-saturated with stirring at 200 rpm and supply of 2 L/min of air at 30° C.) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, 100 μg/mL of streptomycin and 20 g/L of glucose. During fermentation, the temperature was maintained at 30° C., and the pH was maintained at 7.0 by 50% NH.sub.4OH. In addition, the dissolved oxygen (DO) level of was maintained at 40% of the initial DO level by increasing the agitation speed up to 1000 rpm. During the fed-batch fermentation, a feeding solution (700 g/L glucose, 8 g/L MgSO.sub.47H.sub.2O, 20 mg/L FeSO.sub.47H.sub.2O) was supplied using a pH-stat method. When the OD.sub.600 reached 5, 1 mM IPTG was added to the culture medium, and for analysis of heme production, sampling was performed at 8-hour intervals.
(195) In order to analyze the production of heme, 1 mL of a sample was collected from the culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared medium and lysed cell samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(196) As a result, the largest amount of heme (a total of 49.18 mg/L) was produced at 56 hours, and 16.77 mg/L (34.10%) of the produced heme was detected in the medium. The OD.sub.600 reached the maximum value (67.2) at 32 hours (
5-3: Extracellular Free Heme Production by Fed-Batch Fermentation of Strain Supplied with (NH.SUB.4.).SUB.2.SO.SUB.4
(197) Based on the fact that 4 nitrogen atoms are required to produce one molecule of heme, it was concluded that the production of heme can be increased by supplying a nitrogen source. To verify the hypothesis, fed-hatch fermentation was performed while supplying (NH.sub.4).sub.2SO.sub.4 as a nitrogen source.
(198) The strain shown in Table 23 was inoculated into 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin and 100 μg/mL of streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the medium in which the cultured cells were growing was inoculated into 200 mL of fresh MR-Fe20 medium supplemented with 25 μg/mL of kanamycin, 50 of μg/mL ampicillin and 100 μg/mL of streptomycin, and then the cells were cultured in a 500-mL Erlenmeyer flask at 37° C. and 200 rpm for 12 hours. Next, the culture was inoculated into a 6.6-L Bioflo3000 fermenter (New Brunswick Scientific Co., Edison, N.J.) containing 1.8 L of MR-Fe20 medium (pH7.0; air-saturated with stirring at 200 rpm and supply of 2 L/min of air at 30° C.) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, 100 μg/mL of streptomycin and 20 g/L of glucose. During fermentation, the temperature was maintained at 30° C., and the pH was maintained at 7.0 by 50% NH.sub.4OH. In addition, the dissolved oxygen (DO) level was maintained at 40% of the initial DO level by increasing the agitation speed up to 1000 rpm. During the fed-batch fermentation, a feeding solution (700 g/L glucose, 8 g/L MgSO.sub.47H.sub.2O, 20 mg/L FeSO.sub.47H.sub.2O) was supplied using a pH-stat method. When the OD.sub.600 reached 5, 1 mM IPTG was added to the culture medium, and for analysis of heme production, sampling was performed at 8-hour intervals. In order to analyze the production of heme, 1 mL of a sample was collected from the culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared medium and lysed cell samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(199) As a result, the largest amount of heme (a total of 104.90 mg/L) was produced at 56 hours, and 54.61 mg/L (52.06%) of the produced heme was detected in the medium. The OD.sub.600 reached the maximum value (93.8) at 48 hours (FIG.8 in graph (b)).
5-4: Extracellular Free Heme Production by Fed-Batch Fermentation of Strain Supplied with Glutamate
(200) The HEME6 strain constructed in the present invention has the advantage of showing the world's highest heme production without supplying ALA production precursors as proven in the above Examples. Glutamate, precursor of the C5 ALA biosynthetic pathway, is relatively inexpensive and shows no cytotoxicity, unlike glycine, precursor of the C4 ALA biosynthetic pathway, which is relatively expensive and cytotoxic. Therefore, in order to examine the maximum heme-producing capacity of the HEME6 strain constructed in the present invention, fed-batch fermentation was performed while supplying glutamate as a nitrogen source.
(201) The strain shown in Table 23 was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin and 100 μg/mL of streptomycin, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 200 mL of fresh MR-Fe20 medium supplemented with 25 μg/mL of kanamycin, 50 of μg/mL ampicillin and 100 μg/mL of streptomycin, and then the cells were cultured in a 500-mL Erlenmeyer flask at 37° C. and 200 rpm for 12 hours. Next, the culture was inoculated into a 6.6-L Bioflo3000 fermenter (New Brunswick Scientific Co., Edison, N.J.) containing 1.8 L of MR-Fe20 medium (pH7.0; air-saturated with stirring at 200 rpm and supply of 2 L/min of air at 30° C.) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, 100 μg/mL of streptomycin and 20 g/L of glucose. During fermentation, the temperature was maintained at 30° C., and the pH was maintained at 7.0 by 50% NH.sub.4OH. In addition, the dissolved oxygen (DO) level was maintained at 40% of the initial DO level by increasing the agitation speed up to 1000 rpm. During the fed-batch fermentation, a feeding solution (700 g/L glucose, 8 g/L MgSO.sub.47H.sub.2O, 20 mg/L FeSO.sub.47H.sub.2O, 20 g/L glutamate) was supplied using a pH-stat method. When the OD.sub.600 reached 5, 1 mM IPTG was added to the culture medium, and for analysis of heme production, sampling was performed at 8-hour intervals.
(202) In order to analyze the production of heme, 1 mL of a sample was collected from the culture medium and centrifuged, and the collected cells were resuspended in 500 μL of 1 M NaOH aqueous solution and lysed by ultrasonic disruption. The prepared medium and lysed cell samples were filtered, and then the concentrations of heme therein were measured by a previously known method using HPLC (Lee et al., J. Microbiol. Biotechnol. 22, 1653-1658, 2012).
(203) As a result, the largest amount of heme (a total of 228.46 mg/L) was produced at 64 hours, and 131.90 mg/L (57.734%) of the produced heme was detected in the medium. The OD.sub.600 reached the maximum value (172.8) at 48 hours (
Example 6: Increased Production of Heme and Promotion of Extracellular Secretion of Heme by Overexpression of Heme Exporter
6-1: Construction of Strain Overexpressing Heme Exporter Gene=ABC
(204) In Example 5, it could be confirmed that as the total production of heme increased, the proportion of extracellularly secreted heme increased gradually. This supports the hypothesis postulated prior to the start of the present invention that when cytotoxic heme (Anzaldi et al., Infect. Immun. 78, 4977-4989, 2010) is overproduced, extracellular heme secretion by the heme exporter CcmABC (Schulz et al., Proc. Natl. Acad. Sci. USA 96, 6462-6467, 1999) can be promoted to secrete free heme to the extracellular space. In addition, it was concluded that when the ccmABC genes are overexpressed in the same context, the extracellular secretion of heme can further be promoted. Thus, a strain overexpressing the ccmABC genes was constructed through the following procedure.
(205) (a) Construction of Plasmid pACYC-ccmABC
(206) The plasmid pACYC-ccmABC was constructed which overexpresses the heme exporter-encoding ccmA gene (SEQ ID NO: 58), ccmB gene (SEQ ID NO: 59) and ccmC gene (SEQ ID NO: 60) through the following procedures.
(207) The primers of SEQ ID NOs: 61 and 62, the primers of SEQ ID NOs: 63 and 64, and the primers of SEQ ID NOs: 65 and 66 were respectively used to amplify the ccmA, ccmB and ccmC genes from the genomic DNA of the E. coli BL21(DE3) strain.
(208) At this time, the sequence of a ribosome binding site (RBS) was added to the 5′ end of the amplified ccmC gene by the sequence of the ribosome binding site (RBS) contained in the primer of SEQ ID NO: 65 used in the amplification process.
(209) TABLE-US-00024 TABLE 24 SEQ ID NOs Nucleotide sequences SEQ ID 5′-GGGAATTCCA TATGGGTATG CTTGAAGCCA NO: 61 GAGAGTT-3′ SEQ ID 5′-CCGCTCGAGT CATGCGGCCC TCGTTT-3′ NO: 62 SEQ ID 5′-CATGCCATGG TGTTCTGGCG CATT-3′ NO: 63 SEQ ID 5′-CGCGGATCCT TATTGAATGC TGATTCGTAA NO: 64 C-3′ SEQ ID 5′-CCGGAATTCA TAAAAGGAGG AAAATATATG NO: 65 TGGAAAACAC TGCATCAAC-3′ SEQ ID 5′-ACGCGTCGAC TCATTTACGG CCTCTTTTCA NO: 66 G-3′
(210) The amplified sequences were cleaved with NcoI and BamHI, EcoRI and SalI, and NdeI and XhoI, respectively, and then sequentially inserted into a pACYCDuet-1 vector (Novagen, USA) cleaved with the same restriction enzymes, thereby constructing pACYC-ccmABC.
(211) (b) Construction of Recombinant E. coli HEME7
(212) The constructed pACYC-ccmABC plasmid was introduced together into the HEME6 strain shown in Table 23, thereby constructing E. coli HEME7 strain (Table 25).
(213) TABLE-US-00025 TABLE 25 Strain Description HEME7 E. coli BL21(DE3) harboring pCDF-hemAL, pRSF-hemBCD, pET-hemEFGH and pACYC-ccmABC
6-2: Extracellular Free Heme Production by Fed-Batch Fermentation of HEME7 Strain Supplied with (NH.SUB.4.).SUB.2.SO.SUB.4
(214) In order to confirm the heme-producing ability of the HEME7 strain overexpressing the heme exporter genes ccmABC, constructed in Example 6-1, fed-batch fermentation was performed while supplying (NH.sub.4).sub.2SO.sub.4 as a nitrogen source.
(215) The strain shown in Table 25 was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, 100 μg/mL of streptomycin, and 17 μg/mL chloramphenicol, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 200 mL of fresh MR-Fe20 medium supplemented with 25 μg/mL of kanamycin, 50 of μg/mL ampicillin, 100 μg/mL of streptomycin, and 17 μg/mL chloramphenicol and then the cells were cultured in a 500-mL Erlenmeyer flask at 37° C. and 200 rpm for 12 hours. Next, the culture was transferred to a 6.6-L Bioflo3000 fermenter (New Brunswick Scientific Co., Edison, N.J.) containing 1.8 L of MR-Fe20 medium (pH7.0; air-saturated with stirring at 200 rpm and supply of 2 L/min of air at 30° C.) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, 100 μg/mL of streptomycin, 17 μg/mL chloramphenicol, and 20 g/L of glucose. During fermentation, the temperature was maintained at 30° C., and the pH was maintained at 7.0 by 50% NH.sub.4OH. In addition, the dissolved oxygen (DO) level was maintained at 40% of the initial DO level by increasing the agitation speed up to 1000 rpm. During the fed-batch fermentation, a feeding solution (700 g/L glucose, 8 g/L MgSO.sub.47H.sub.2O, 20 mg/L FeSO.sub.47H.sub.2O, 5 g/L (NH.sub.4) 2504) was supplied using a pH-stat method. When the OD.sub.600 reached 5, 1 mM IPTG was added to the culture medium, and for analysis of heme production, sampling was performed at 8-hour intervals to confirm the production of heme.
(216) As a result, the largest amount of heme (a total of 114.79 mg/L) was produced at 64 hours, and 71.91 mg/L (62.64%) of the produced heme was detected in the medium. The OD.sub.600 reached the maximum value (76.9) at 56 hours (
6-3: Extracellular Free Heme Production by Fed-Batch Fermentation of HEME7 Strain Supplied with Glutamate
(217) In order to examine the highest heme production when the precursor of the C5 ALA biosynthetic pathway of the HEME7 strain overexpressing the heme exporter genes ccmABC, constructed in Example 6-1, was supplied, fed-batch fermentation was performed while supplying glutamate as a nitrogen source.
(218) The strain shown in Table 25 was used to inoculate 5 mL of LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, 100 μg/mL of streptomycin, and 17 μg/mL chloramphenicol, and was then cultured at 37° C. and 220 rpm for 12 hours. For main culture for heme production, 1 mL of the pre-culture was transferred to 200 mL of fresh MR-Fe20 medium supplemented with 25 μg/mL of kanamycin, 50 of μg/mL ampicillin, 100 μg/mL of streptomycin, and 17 μg/mL chloramphenicol and then the cells were cultured in a 500-mL Erlenmeyer flask at 37° C. and 200 rpm for 12 hours. Next, the culture was transferred to a 6.6-L Bioflo3000 fermenter (New Brunswick Scientific Co., Edison, N.J.) containing 1.8 L of MR-Fe20 medium (pH7.0; air-saturated with stirring at 200 rpm and supply of 2 L/min of air at 30° C.) supplemented with 25 μg/mL of kanamycin, 50 μg/mL of ampicillin, 100 μg/mL of streptomycin, 17 μg/mL chloramphenicol, and 20 g/L of glucose. During fermentation, the temperature was maintained at 30° C., and the pH was maintained at 7.0 by 50% NH.sub.4OH. In addition, the dissolved oxygen (DO) level was maintained at 40% of the initial DO level by increasing the agitation speed up to 1000 rpm. During the fed-batch fermentation, a feeding solution (700 g/L glucose, 8 g/L MgSO.sub.47H.sub.2O, 20 mg/L FeSO.sub.47H.sub.2O, 20 g/L glutamate) was supplied using a pH-stat method for continuous glucose supply. When the OD.sub.600 reached 5, 1 mM IPTG was added to the culture medium, and for analysis of heme production, sampling was performed at 8-hour intervals to confirm the production of heme.
(219) As a result, the largest amount of heme (a total of 246.69 mg/L) was produced at 72 hours, and 164.12 mg/L (66.529%) of the produced heme was detected in the medium. The OD.sub.600 reached the maximum value (142.8) at 64 hours (
Example 7: Production of Heme in Corynebacterium glutamicum
(220) In order to examine whether the strategy used to develop the E. coli strain that produces and extracellularly secretes free heme may also be applied for the production of heme in other strains, the C5 ALA biosynthetic pathway was introduced into Corynebacterium glutamicum which is widely used for the production of amino acids including glutamic acid due to its proven harmlessness to the human body, and heme was produced.
(221) C. glutamicum, an aerobic microorganism overexpressing glutamate, has the C5 biosynthetic pathway that produces ALA from glutamate and the heme biosynthetic pathway that produces heme from ALA, similar to E. coli. To overexpress the hemA and hemL genes of C. glutamicum, which correspond to the C5 biosynthetic pathway, the hemA and hemL genes were amplified from the genomic DNA of C. glutamicum ATCC 13032 using the primers of SEQ ID NOs: 67 and 68 and the primers of SEQ ID NOs: 69 and 70, respectively. In this process, two amino acids (lysine) were inserted between the second amino acid and the third amino acid of the protein expressed from the hemA gene, and thus the sequence of the hemA gene was modified such that the HemA protein would show resistance to negative feedback (HemA.sup.fbr). The two amplified genes were cloned together into the EcoRI site of a pEKEx1 plasmid by Gibson assembly, thereby constructing a pEKEx1-hemAL plasmid which expresses the HemA.sup.fbr and HemL proteins when introduced into C. glutamicum.
(222) TABLE-US-00026 TABLE 26 SEQ ID NOs Nucleotide sequences SEQ ID 5′-TGAGCGGATA ACAATTTCAC ACAGGAAACA NO: 67 GAATTCATGG TGAGTAAGAA GGTACTCATC GTAGGGATGT CGCACA-3′ SEQ ID 5′-GCGGATCGAG CCGTATTGGA CGATGTCATT NO: 68 GTTTCCTGTG TGAAATTACT CCCTCGTTTG TGTGGCAGAA-3′ SEQ ID 5′-CACCTTCTGC CACACAAACG AGGGAGTAAT NO: 69 TTCACACAGG AAACAATGAC ATCGTCCAAT ACGGCTCGAT-3′ SEQ ID 5′-GCCAAGCTTG GCTGCAGGTC GACGGATCCC NO: 70 CGGGAATTCT CATGATGCCT TCGCTTCTGC TGCT-3′
(223) In addition, in order to examine the overexpression of the hemB and hemH genes among the genes encoding the proteins involved in producing heme from ALA as shown in (a) of Example 2-1 above, a vector overexpressing the hemB and hemH genes was constructed. To this end, the hemB and hemH genes were amplified from the genomic DNA of C. glutamicum ATCC 13032 using the primers of SEQ ID NOs: 71 and 72 and the primers of SEQ ID NOs: 73 and 74, respectively. In addition, a pCES208-spc plasmid was amplified using the primers of SEQ ID NOs: 75 and 76, and then assembled with the amplified hemB and hemH genes by Gibson assembly, thereby constructing a pCES-hemBH plasmid.
(224) TABLE-US-00027 TABLE 27 SEQ ID NOs Nucleotide sequences SEQ ID 5′-GCACCTTGGT TGGTAGGAGT AGCATGGGAT NO: 71 CCATGCATCA CCATCACCAT CATAGCACTT CTTCTGATTA CTCCCACG-3′ SEQ ID 5′-TTGTTTCCTG TGTGAAATTA AGCGTTTCGC NO: 72 AGTGCGCGGG-3′ SEQ ID 5′-TAATTTCACA CAGGAAACAA TGAATGAACG NO: 73 CACATCGGATGC-3′ SEQ ID 5′-AATTATAATG GCCGGCTGGG CCTCTAGACT NO: 74 AGTTGGCAGC TGGCGCCGCT GA-3′ SEQ ID 5′-TCTAGAGGCC CAGCCGGCCA TTATAATTAG-3′ NO: 75 SEQ ID 5′-GGATCCCATG CTACTCCTAC CAACCAAGGT-3′ NO: 76
(225) The constructed pEKEx1-hemAL plasmid or two plasmids (pEKEx1-hemAL and pCES-hemBH) were introduced into the industrial C. glutamicum 5112 strain, thereby constructing C. glutamicum S112-AL and S112-AL-BH strains.
(226) TABLE-US-00028 TABLE 28 Strain Description S112-AL C. glutamicum S112 harboring pEKEx1-hemAL S112-AL-BH C. glutamicum S112 harboring pEKEx1-hemAL and pCES-hemBH
(227) For flask culture for examining the heme-producing abilities of the two constructed C. glutamicum strains (S112-A1 and S112-AL-BH), each of the strains was used to inoculate 10 mL of a seed medium having the composition of Table 29 below, contained in a 250-mL baffled flask, and was then cultured at 30° C. and 200 rpm for about 12 hours until the OD.sub.600 reached 20.
(228) TABLE-US-00029 TABLE 29 Component Concentration Glucose 40.0 g/L MgSO.sub.47H.sub.2O 0.4 g/L FeSO.sub.47H.sub.2O 10.0 mg/L MnSO.sub.45H.sub.2O 10.0 mg/L KH.sub.2PO.sub.4 1.0 g/L Urea 4.0 g/L Thiamine-HCl 200 μg/L Biotin 50.0 μg/L Soy bean hydrolysate 55.0 mL/L pH 7.0
(229) Next, 100 μL of the culture was inoculated into 10 mL of a main culture medium having the composition of Table 30 below, contained in a 250-mL baffled flask, and was then cultured at 30° C. and 200 rpm for 48 hours.
(230) TABLE-US-00030 TABLE 30 Component Concentration Glucose 70.0 g/L MgSO.sub.47H.sub.2O 0.4 g/L FeSO.sub.47H.sub.2O 10.0 mg/L MnSO.sub.45H.sub.2O 10.0 mg/L (NH.sub.4).sub.2SO.sub.4 30.0 g/L KH.sub.2PO.sub.4 1.0 g/L Thiamine-HCl 200 μg/L Biotin 50.0 μg/L Soy bean hydrolysate 55.0 mL/L CaCO.sub.3 50.0 g/L pH 8.0
(231) As a result, it was confirmed that in C. glutamicum 5112 that does not overexpress the heme biosynthesis-related genes, heme was not produced, whereas, in the S112-AL and S112-AL-BH strains, heme accumulated in the cells in amounts of 0.77±0.23 and 1.64±0.30 mg/L (based on the culture volume), respectively. Therefore, it was confirmed that heme can be produced even in C. glutamicum by overexpressing the C5 biosynthetic pathway and the heme biosynthetic pathway.
INDUSTRIAL APPLICABILITY
(232) According to the present invention, heme, an organometallic compound increasingly used as a health food or food supplement for the treatment of porphyria, can be extracellularly secreted and produced in high yield using the microorganism variant, but not conventional chemical synthesis or enzymatic synthesis, and thus a purification process required to use the produced heme can be performed in an environmentally friendly, economical and easy manner.
(233) Although the present invention has been described in detail with reference to the specific features, it will be apparent to those skilled in the art that this description is only for a preferred embodiment and does not limit the scope of the present invention. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.