Solid mask and preparation method therefor

Abstract

The present invention provides a solid mask and a preparation method thereof. The solid mask includes a hydrophobic substrate layer and a nanofiber layer, the nanofiber layer has a three-dimensional structure and is electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer is prepared by the following food-grade raw materials in parts by mass: 10 to 30 parts of gelatin, 1 to 30 parts of soya bean lecithin and 0.1 to 10 parts of a functional substance. The present invention provides a solid mask, using gelatin and soya bean lecithin as the framework. The nanofiber layer is a three-dimensional laminate made of fibers having a diameter of a few hundred nanometers. The nanofiber layer has a membrane structure similar to the extracellular matrix. The raw materials of the solid mask are all food-grade raw materials or natural extracts.

Claims

1. A solid mask, comprising a hydrophobic substrate layer and a nanofiber layer, the nanofiber layer has a three-dimensional structure and is electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer is prepared by the following food-grade raw materials in parts by mass: 10 to 30 parts of gelatin, 1 to 30 parts of soya bean lecithin and 0.1 to 10 parts of a functional substance.

2. The solid mask according to claim 1, wherein the nanofiber layer is prepared by the following food-grade raw materials in parts by mass: 18 to 26 parts of gelatin, 2 to 15 parts of soya bean lecithin and 0.2 to 7.5 parts of the functional substance.

3. The solid mask according to claim 2, wherein the nanofiber layer is prepared by the following food-grade raw materials in parts by mass: 24 parts of gelatin, 5 parts of soya bean lecithin and 7.2 parts of the functional substance.

4. The solid mask according to claim 1, wherein the functional substance comprises one or more of white tremella polysaccharide, Saussurea polysaccharide, Dendrobium officinals Kimura et Migo, sodium hyaluronate, glycerin, nicotinamide, collagen peptide powder, collagen tripeptide, carnosine and hydrolyzed collagen.

5. The solid mask according to claim 4, wherein the functional substance is a mixture of hydrolyzed collagen, collagen peptide powder, collagen tripeptide and carnosine, wherein a mass ratio of hydrolyzed collagen to collagen peptide powder to collagen tripeptide to carnosine is 1:5:1:0.2.

6. The solid mask according to claim 5, wherein the collagen peptide powder has an average molecular weight of 2000 Dalton, and the collagen tripeptide has an average molecular weight of 280 Dalton.

7. The solid mask according to claim 1, wherein nanofibers of the nanofiber layer have a diameter of 80 nm to 800 nm, and the nanofiber layer has a thickness of 0.05 mm to 2 mm.

8. The solid mask according to claim 2, wherein the functional substance comprises one or more of white tremella polysaccharide, Saussurea polysaccharide, Dendrobium officinale Kimura et Migo, sodium hyaluronate, glycerin, nicotinamide, collagen peptide powder, collagen tripeptide, carnosine and hydrolyzed collagen.

9. The solid mask according to claim 8, wherein the functional substance is a mixture of hydrolyzed collagen, collagen peptide powder, collagen tripeptide and carnosine, wherein a mass ratio of hydrolyzed collagen to collagen peptide powder to collagen tripeptide to carnosine is 1:5:1:0.2.

10. The solid mask according to claim 9, wherein the collagen peptide powder has an average molecular weight of 2000 Dalton, and the collagen tripeptide has an average molecular weight of 280 Dalton.

11. The solid mask according to claim 3, wherein the functional substance comprises one or more of white tremella polysaccharide, Saussurea polysaccharide, Dendrobium officinale Kimura et Migo, sodium hyaluronate, glycerin, nicotinamide, collagen peptide powder, collagen tripeptide, carnosine and hydrolyzed collagen.

12. The solid mask according to claim 11, wherein the functional substance is a mixture of hydrolyzed collagen, collagen peptide powder, collagen tripeptide and carnosine, wherein a mass ratio of hydrolyzed collagen to collagen peptide powder to collagen tripeptide to carnosine is 1:5:1:0.2.

13. The solid mask according to claim 12, wherein the collagen peptide powder has an average molecular weight of 2000 Dalton, and the collagen tripeptide has an average molecular weight of 280 Dalton.

14. A preparation method for the solid mask according to claim 1, the preparation method comprises the following steps: step 1, preparation of electrospinning solution: adding gelatin, soya bean lecithin and the functional substance to a solvent, followed by mixing to form an even electrospinning solution; step 2, electrostatic spinning: spinning the electrospinning solution onto the hydrophobic substrate layer by an electrostatic spinning device to prepare and obtain the solid mask, with a working voltage of 10 kV to 28 kV, a feeding velocity of 0.1 mL/hr to 2 mL/hr, a spinning distance of 6 cm to 25 cm, and a relative humidity of working environment of 30% to 50%.

15. The preparation method for the solid mask according to claim 14, wherein in the step 2, the working voltage for the electrostatic spinning is 15 kV to 23 kV, the feeding velocity is 0.4 mL/hr to 1 mL/hr, the spinning distance is 10 cm to 18 cm, and the relative humidity of working environment is 35% to 45%.

16. The preparation method for the solid mask according to claim 14, wherein the solvent is 50% to 90% acetic acid solution.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 shows scanning electron micrographs of electrospinning masks doped with different functional components, wherein A of FIG. 1 to L of FIG. 1 are the electrospinning masks doped with the functional components S1 to S12 in Table 1.

(2) FIG. 2 shows the effects of a solution of solid mask S1 of different concentrations on proliferation rates of human normal skin fibroblasts (A of FIG. 2) and human immortalized keratinocytes (B of FIG. 2).

(3) FIG. 3 shows the anti-oxidation performance of the solid mask S1 on the human normal skin fibroblasts (A of FIG. 3) and the human immortalized keratinocytes (B of FIG. 3).

(4) A of FIG. 4 shows curves of total cumulative penetration amounts of protein of a raw material solution (a) and the solid mask S1 (b) varying with transdermal time; B of FIG. 4 shows retention amounts of protein of the raw material solution and the solid mask S1, in rat skin.

DETAILED DESCRIPTION

(5) The present invention is further described hereinafter with reference to the specific examples, but the examples are not intended to limit the invention in any form. Unless defined otherwise, the raw material reagents used in the examples of the invention are conventionally purchased raw material reagents.

Example 1

(6) A solid mask, included a non-woven substrate layer and a nanofiber layer, wherein the nanofiber layer had a three-dimensional structure and was electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer was prepared by the following materials in parts by mass: 24 parts of gelatin, 5 parts of soya bean lecithin and 7.2 parts of functional substance shown in Table 1.

(7) TABLE-US-00001 TABLE 1 Functional components of different electrospinning solutions number Functional components  S1 1% hydrolyzed collagen, 5% collagen peptide powder (2000 Dalton), 1% collagen tripeptide (molecular weight: 280 Dalton), 0.2% carnosine  S2 —  S3 1% hydrolyzed collagen  S4 5% collagen peptide powder (molecular weight: 2000 Dalton)  S5 5% collagen tripeptide (molecular weight: 280 Dalton)  S6 5% carnosine  S7 1% white tremella polysaccharide  S8 1% Saussurea polysaccharide  S9 1% extract of Dendrobium officinale Kimura et Migo S10 1% sodium hyaluronate S11 1% nicotinamide S12 1% glycerin

(8) A preparation method includes the following steps:

(9) step 1, preparation of electrospinning solution: using 70% (volume fraction) acetic acid solution as a solvent, adding gelatin, soya bean lecithin and a functional substance to the solvent, followed by mixing to form an even electrospinning solution;

(10) step 2, electrostatic spinning: fixing the non-woven substrate layer to the roller, spinning the electrospinning solution of each group onto the hydrophobic substrate layer by an electrostatic spinning device to prepare and obtain solid masks, with a working voltage of 18 kV, a feeding velocity of 0.5 mL/hr, a spinning distance of 12 cm, and a relative humidity of working environment of 35% to 40%.

(11) After a period of electrostatic spinning, the spun hydrophobic substrate layer was removed from the roller and cut by a mask cutter, and then the mask was folded into a quarter size and sealed in an aluminum foil packing bag for later use.

(12) The nanofiber layer prepared had a fiber diameter ranging from 80 nm to 800 nm, approximately 200 nm in average, and a fiber layer thickness of 0.05 mm to 2 mm, 0.5 mm in majority.

(13) Performance Determination:

(14) The results proves that various functional substances of whitening, moisturizing and anti-aging can be all loaded to the instant-dissolved mask which used gelatin and soy bean lecithin as a framework, to prepare the solid masks with various effects.

(15) (1) Morphology Determination

(16) Scanning electron micrographs of the electrospinning masks doped with different functional components are shown as FIG. 1. It can be seen from FIG. 1 that the masks which were prepared by the electrospinning solutions with different functional components are nanofiber membranes composed of uniform nanofibers, and thus the solid masks have extremely high specific surface area capable of absorbing water fast and becoming invisible. It should be understood that the term invisible in the present invention means that after absorbing water, the solid mask would be dissolved rapidly and its initial solid form exists no more, and thereby even and smooth fibers are formed. The results of the scanning electron micrographs prove that uniform and smooth fibers were formed, without any functional substances leaking out from the fiber layer surface.

(17) (2) Cellular Affinity Test

(18) Cell planking: human normal skin fibroblasts (HSF) and human immortalized keratinocytes (Hacat) were planked into a 96-well plate with concentrations of 6000 cells and 12000 cells respectively, and cultured for 24 hours for later use.

(19) Sterilization of masks and preparation of samples: on a super clean bench, the front side and the rear side of a mask were subjected to UV for 30 minutes for sterilization. The sterilized mask was dissolved in a culture medium containing blood serum to the maximum concentration of 20 mg/mL, and diluted in sequence to obtain samples in concentrations of 20, 10, 5, 3, 1, 0.5, 0.3, 0.1 and 0.05 mg/mL.

(20) Sample loading: culture solutions in the 96-well plates with HSF and Hacat cells cultured for 24 hours therein were taken and added to the culture medium containing different concentrations of samples (sample groups); meanwhile, a control group was established, that is, a group of cells and normal culture medium containing blood serum; and a background deduction group was established, that is, a group of culture medium without planking cells, for deducting interference of the culture plate. Each group was provided with five parallel wells, and 100 μL of sample was added to each well.

(21) Determination of cellular proliferation rate: after loading the samples, the culture mediums were cultured in the incubator for 24 hours, and then taken out for determination of cellular proliferation rate by the SRB (Sulforhodamine B) method.

(22) Determination results are shown in FIG. 2. FIG. 2 indicates that the mask solutions of different concentrations had no impact on the proliferation of the HSF and Hacat cells, and to some extent accelerated the growth of cells, indicating a very good cellular affinity. With the concentration increasing, cellular activity was enhanced. However, if the concentration of the electrospinning mask solution was further increased, the culture solution got thick which had impact on the growth of cells, thereby cell viability dropped to some extent. Results of IR and XRD can prove a relatively good compatibility of the functional substances and gelatin.

(23) (3) Hen's Egg Test on the Chorioallantoic Membrane (HET-CAM)

(24) Referring to Cosmetic Ocular Irritant and Corrosive HET-CAM Test Standard issued by the Entry-Exit Inspection and Quarantine Industry Standards, the solid mask of the present invention was first in solid state but dissolved fast when in contact with water, and thus an irritant grading method for transparent liquid subject and a terminal point grading method for solid such as particles and paste and turbid liquid subject are used to evaluate the irritation and corrosiveness of the electrospinning solid mask.

(25) Irritant grading method: 0.3 mL of 20 mg/mL solid mask solution was added to the surface of a chorioallantoic membrane (CAM), the time of starting hemorrhage, blood vessel lysis and coagulation within 5 minutes was recorded, and six parallel tests were carried out.

(26) Terminal point grading method: 30 mg of solid mask was directly applied to the CAM, and after 3 minutes, the residual mask was slightly washed off the CAM with normal saline, and condition of the CAM was observed and graded. Six parallel tests were carried out.

(27) After in contact with the 20 mg/mL solid mask solution for 5 minutes, no substantial change was found in the CAM, and result of the irritant grading was non-irritant; after 30 mg of the electrospinning invisible mask was directly in contact with the CAM for 3 minutes, the residual mask was slightly washed off the CAM with normal saline, no hemorrhage, blood vessel lysis or coagulation occurred on the CAM subjected to contact with the dry mask, and thus the terminal point grading was 0 indicating that the mask was evaluated as non-irritant. The results all prove that the solid mask has good biocompatibility.

(28) (4) Anti-Oxidation Test

(29) Cell planking: HSF and Hacat cells were planked into a 96-well plate with concentrations of 6000 cells and 12000 cells respectively, and cultured for 24 hours for later use.

(30) Sterilization and preparation of hydrogen peroxide solution and mask samples: on a super clean bench, hydrogen peroxide was dissolved in a culture solution to prepare a solution in concentration of 600 μmon, and the solution was subjected to filtration and sterilization with a 0.22 μm filter for later use. The front side and the rear side of mask S1 were subjected to UV for 30 minutes for sterilization. The sterilized mask was dissolved in the culture solution containing H.sub.2O.sub.2 to prepare a 1 mg/mL sample for later use.

(31) Sample loading: culture solutions in the 96-well plates with HSF and Hacat cells cultured for 24 hours therein were taken and added to the culture solution containing 600 μmon H.sub.2O.sub.2 (H.sub.2O.sub.2 group) and the culture solution containing 600 μmon H.sub.2O.sub.2 and 1 mg/mL mask S1 (H.sub.2O.sub.2+ mask group) in sequence; as the above mentioned, a control group was established at the same time, that is, a group of cells and normal culture solution; and a background deduction group was established, that is, a group of culture solution without planking cells, for deducting interference of the culture plate. Each group was provided with five parallel wells, and 100 μL of sample was added to each well.

(32) Determination of cellular proliferation rate: after loading the samples, the culture mediums were cultured in the incubator for 24 hours, and then taken out for determination of cellular proliferation rate by the SRB method.

(33) HSF and Hacat cells show significantly decreased survival rates after adding hydrogen peroxide to the culture solutions of human normal skin fibroblasts and human immortalized keratinocytes, proving that modeling of hydrogen peroxide damage model is succeeded. When H.sub.2O.sub.2 and the electrospinning invisible mask were added simultaneously to the cellular culture solution, damage to the cell caused by hydrogen peroxide was repaired by the mask, especially the HSF cell of which activity would not be affected by hydrogen peroxide after adding the mask, wherein the cellular survival rate was even above 100% (shown in FIG. 3). It is proved that the electrospinning invisible mask containing carnosine has anti-oxidation performance and activity of skin care, which is capable of repairing the damaged cell membrane.

(34) (5) Transdermal Test

(35) Preparation of rat skin in vitro: abdomen skin of rat was taken, followed by removing the subcutaneous adipose tissue and connective tissue, and washed repeatedly with normal saline until there's no turbidity, for later use.

(36) Preparation of sample: in the first group, the same amount of raw materials as the mask S1 were dissolved in water to prepare a 20 mg/mL raw material solution; and in the second group, 20 mg of the solid mask S1 was provided.

(37) Transdermal test: rat skin was fixed on a diffusion cell of a transdermal test instrument, with the stratum corneum facing upward and the dermis facing an accepting cell. As for the first group, 1 mL of raw material solution was dropwise added to the rat skin; as for the second group, 1 mL of water was dropwise added to the skin first, and 20 mg of the solid mask S1 was applied to the wet rat skin; and then a liquid-feeding cell of each group was sealed with preservative film.

(38) Sample taking and determination: when the transdermal test lasted for 15 minutes, 30 minutes, 1 hour, 2 hours, 4 hours, 6 hours, 8 hours, 12 hours and 24 hours, 0.5 mL of receptor fluid was extracted by a sampling needle respectively and the same amount of fresh receptor fluid was replenished. Transdermal amount of the functional substance was characterized indirectly by determining protein content in the extracted receptor liquid by the BCA method.

(39) Determination method of retention amount of protein: after the transdermal test lasted for 24 hours, the rat skin was removed from the transdermal test instrument, and an effective area of the rat skin was cut off and washed clean with distilled water. After being drained off, the rat skin was cut into pieces with a scissor and placed in a 2 mL centrifuge tube, followed by adding 1 mL of ultrapure water, then subjected to homogenate for 3 minutes to 5 minutes via a homogenizer until there's no visible solid rat skin. After centrifugation, supernatant was filtered by a 0.22 μm filter membrane, and then retention amount of the functional substance in the rat skin was characterized indirectly by determining protein content in the supernatant by the BCA method.

(40) Indirect results are shown in FIG. 4, wherein a represents the first group, and b represents the second group. It can be seen from FIG. 4 that when the transdermal test lasted for 15 minutes, 30 minutes, 1 hour, 2 hours, 4 hours, 6 hours, 8 hours, 12 hours and 24 hours, a total cumulative penetration amount of protein through the rat skin of the second group was far more than that of the first group, accounting for 130% to 160% of the first group. The total cumulative penetration amount of protein through the rat skin of the second group can reach up to approximately 1.8 mg. Also, the retention amount of protein in the rat skin of the second group was far less than that of the first group, and the retention amount of protein in the rat skin of the second group was about 0.6 mg merely. It can be proved from the experimental results that compared with the essence serum, the solid nanofiber mask in the present invention prepared by the electrostatic spinning technology is easier to penetrate the stratum corneum and reach to the deep of the skin, which can improve the utilization rate of the functional substance.

Example 2

(41) A solid mask, included a non-woven substrate layer and a nanofiber layer, wherein the nanofiber layer had a three-dimensional structure and was electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer was prepared by the following materials in parts by mass: 30 parts of gelatin, 1 part of soya bean lecithin and 0.1 part of functional substance shown as S1 in Table 1.

(42) The preparation method includes the following steps:

(43) step 1, preparation of electrospinning solution: using 50% (volume fraction) acetic acid solution as a solvent, adding gelatin, soya bean lecithin and a functional substance to the solvent, followed by mixing to form an even electrospinning solution;

(44) step 2, electrostatic spinning: fixing the non-woven substrate layer to the roller, spinning the electrospinning solution of each group onto the hydrophobic substrate layer by an electrostatic spinning device to prepare and obtain solid masks, with a working voltage of 28 kV, a feeding velocity of 2 mL/hr, a spinning distance of 25 cm, and a relative humidity of working environment of 50%.

(45) After a period of electrostatic spinning, the spun hydrophobic substrate layer was removed from the roller and cut by a mask cutter, and then the mask was folded into a quarter size and sealed in an aluminum foil packing bag for later use.

(46) The nanofiber layer prepared had a fiber diameter ranging from 80 nm to 800 nm, and a fiber layer thickness of 0.05 mm to 2 mm.

Example 3

(47) A solid mask, included a non-woven substrate layer and a nanofiber layer, wherein the nanofiber layer had a three-dimensional structure and was electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer was prepared by the following materials in parts by mass: 10 parts of gelatin, 30 parts of soya bean lecithin and 10 parts of functional substance shown as S1 in Table 1.

(48) The preparation method includes the following steps:

(49) step 1, preparation of electrospinning solution: using 90% (volume fraction) acetic acid solution as a solvent, adding gelatin, soya bean lecithin and a functional substance to the solvent, followed by mixing to form an even electrospinning solution;

(50) step 2, electrostatic spinning: fixing the non-woven substrate layer to the roller, spinning the electrospinning solution of each group onto the hydrophobic substrate layer by an electrostatic spinning device to prepare and obtain solid masks, with a working voltage of 10 kV, a feeding velocity of 0.1 mL/hr, a spinning distance of 6 cm, and a relative humidity of working environment of 30%.

(51) After a period of electrostatic spinning, the spun hydrophobic substrate layer was removed from the roller and cut by a mask cutter, and then the mask was folded into a quarter size and sealed in an aluminum foil packing bag for later use.

(52) The nanofiber layer prepared had a fiber diameter ranging from 80-800 nm, and a fiber layer thickness of 0.05 mm to 2 mm.

Example 4

(53) A solid mask, included a non-woven substrate layer and a nanofiber layer, wherein the nanofiber layer had a three-dimensional structure and was electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer was prepared by the following materials in parts by mass: 18 parts of gelatin, 15 parts of soya bean lecithin and 7.5 parts of functional substance shown as S1 in Table 1.

(54) The preparation method includes the following steps:

(55) step 1, preparation of electrospinning solution: using 90% (volume fraction) acetic acid solution as a solvent, adding gelatin, soya bean lecithin and a functional substance to the solvent, followed by mixing to form an even electrospinning solution;

(56) step 2, electrostatic spinning: fixing the non-woven substrate layer to the roller, spinning the electrospinning solution of each group onto the hydrophobic substrate layer by an electrostatic spinning device to prepare and obtain solid masks, with a working voltage of 15 kV, a feeding velocity of 0.4 mL/hr, a spinning distance of 10 cm, and a relative humidity of working environment of 35%.

(57) After a period of electrostatic spinning, the spun hydrophobic substrate layer was removed from the roller and cut by a mask cutter, and then the mask was folded into a quarter size and sealed in an aluminum foil packing bag for later use.

(58) The nanofiber layer prepared had a fiber diameter ranging from 80 nm to 800 nm, and a fiber layer thickness of 0.05 mm to 2 mm.

Example 5

(59) A solid mask, included a non-woven substrate layer and a nanofiber layer, wherein the nanofiber layer had a three-dimensional structure and was electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer was prepared by the following materials in parts by mass: 26 parts of gelatin, 2 parts of soya bean lecithin and 0.2 part of functional substance shown as S1 in Table 1.

(60) The preparation method includes the following steps:

(61) step 1, preparation of electrospinning solution: using 70% (volume fraction) acetic acid solution as a solvent, adding gelatin, soya bean lecithin and a functional substance to the solvent, followed by mixing to form an even electrospinning solution;

(62) step 2, electrostatic spinning: fixing the non-woven substrate layer to the roller, spinning the electrospinning solution of each group onto the hydrophobic substrate layer by an electrostatic spinning device to prepare and obtain solid masks, with a working voltage of 23 kV, a feeding velocity of 1 mL/hr, a spinning distance of 18 cm, and a relative humidity of working environment of 45%.

(63) After a period of electrostatic spinning, the spun hydrophobic substrate layer was removed from the roller and cut by a mask cutter, and then the mask was folded into a quarter size and sealed in an aluminum foil packing bag for later use.

(64) The nanofiber layer prepared had a fiber diameter ranging from 80 nm to 800 nm, and a fiber layer thickness of 0.05 mm to 2 mm.

Comparative Example 1

(65) A solid mask, included a non-woven substrate layer and a nanofiber layer, wherein the nanofiber layer had a three-dimensional structure and was electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer was prepared by the following materials in parts by mass: 29 parts of gelatin and 7.2 parts of functional substance shown as S1 in Table 1.

(66) The preparation method was the same as that in Example 1.

(67) The nanofiber layer prepared had a fiber diameter ranging from 80 nm to 800 nm, and a fiber layer thickness of 0.05 mm to 2 mm.

Comparative Example 2

(68) A solid mask, included a non-woven substrate layer and a nanofiber layer, wherein the nanofiber layer had a three-dimensional structure and was electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer was prepared by the following materials in parts by mass: 29 parts of soya bean lecithin and 7.2 parts of functional substance shown as S1 in Table 1.

(69) The preparation method was the same as that in Example 1.

(70) The nanofiber layer prepared showed appearances of fractured yarns and holes, having a fiber diameter ranging from 80 nm to 800 nm, and a fiber layer thickness of 0.05 mm to 2 mm.

Comparative Example 3

(71) A solid mask, included a non-woven substrate layer and a nanofiber layer, wherein the nanofiber layer had a three-dimensional structure and was electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer was prepared by the following materials in parts by mass: 8 parts of gelatin, 5 parts of soya bean lecithin and 7.2 parts of functional substance shown as S1 in Table 1.

(72) The preparation method was the same as that in Example 1.

(73) The nanofiber layer prepared showed appearances of fractured yarns and holes, having a fiber diameter ranging from 80 nm to 800 nm, and a fiber layer thickness of 0.05 mm to 2 mm.

Comparative Example 4

(74) A solid mask, included a non-woven substrate layer and a nanofiber layer, wherein the nanofiber layer had a three-dimensional structure and was electro-spun onto the hydrophobic substrate layer through uniaxial electrostatic spinning technology, and the nanofiber layer was prepared by the following materials in parts by mass: 35 parts of gelatin, 5 parts of soya bean lecithin and 7.2 parts of functional substance shown as S1 in Table 1.

(75) The preparation method was the same as that in Example 1.

(76) The nanofiber layer prepared showed appearances of fractured yarns and holes, having a fiber diameter ranging from 80 nm to 800 nm, and a fiber layer thickness of 0.05 mm to 2 mm.

(77) Test Results

(78) Particularly, results of the transdermal test of Examples 2-5 and Comparative Examples 1-4 are shown in Table 2:

(79) TABLE-US-00002 TABLE 2 Total cumulative penetration amount of protein (mg) Number 15 min 30 min l hr 2 hr 4 hr 6 hr 8 hr 12 hr 24 hr Example 2 0.23 0.27 0.31 0.45 0.7 0.77 0.9 1.1 1.4 Example 3 0.24 0.28 0.32 0.47 0.72 0.78 0.92 1.12 1.45 Example 4 0.27 0.31 0.39 0.54 0.83 0.94 1.14 1.25 1.69 Example 5 0.26 0.29 0.37 0.49 0.78 0.86 1.05 1.21 1.57 Comparative 0.23 0.26 0.3 0.45 0.68 0.76 0.86 1.02 1.35 Example 1 Comparative 0.22 0.24 0.28 0.42 0.63 0.7 0.82 0.96 1.32 Example 2 Comparative 0.19 0.21 0.26 0.34 0.5 0.66 0.74 0.97 1.15 Example 3 Comparative 0.17 0.18 0.24 0.28 0.42 0.56 0.62 0.81 1.12 Example 4

(80) TABLE-US-00003 TABLE 3 Total retention amount of protein in the skin Total retention amount of Number protein in the skin/mg Example 2 0.79 Example 3 0.78 Example 4 0.65 Example 5 0.67 Comparative 0.8  Example 1 Comparative 0.82 Example 2 Comparative 0.86 Example 3 Comparative 0.89 Example 4

(81) It can be seen from Table 2 and Table 3 that only when the material proportions and electrospinning parameters are in the optimized range, a nanofiber layer having high specific surface area and formed by nanofibers of even size may be obtained. In addition, within such range, nanoparticles and liposomes may be self-assembled which are conducive to transdermal absorption of the functional substances. If the formed nanofibers were agglomerated into clusters or dots, or had fractured yarns or holes, structure of the nanofiber layer would be severely affected; and too much or too less lecithin would go against the self-assembly, thereby, on the contrary, blocking pores and decreasing the transdermal absorption rate of functional substance.

(82) Obviously, the above-mentioned embodiments of the invention are merely examples for clearly illustrating the invention, but are not intended to limit the implementations of the invention. For those of ordinary skills in the art, other different forms of changes or variations can be made on the basis of the above description. It is not necessary or possible to exhaust all the embodiments here. Any change, equivalent substitution, and improvement made within the spirit and principle of the invention shall fall within the protection scope of the claims of the invention.