Compound having anticancer activity and preparation method and application
11351151 · 2022-06-07
Assignee
Inventors
Cpc classification
C07K7/02
CHEMISTRY; METALLURGY
Y02P20/55
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C07D231/56
CHEMISTRY; METALLURGY
A61P1/16
HUMAN NECESSITIES
A61K31/416
HUMAN NECESSITIES
A61P35/00
HUMAN NECESSITIES
International classification
Abstract
The present invention provides a compound exemplified by Formula I: ##STR00001##
a process for its preparation and the use of a medicament for the treatment of cancer. The compound of the present invention has an inhibitory effect on various cancer cells and can be biologically converted into the active drug Linifanib in vitro (in liver homogenate and spleen homogenate) to inhibit the proliferation of tumor cells, especially liver cancer cells, at a lower dose.
Claims
1. A pharmaceutical composition comprising a compound of the structure of Formula I, a pharmaceutically acceptable salt, stereoisomer or solvate thereof, and a pharmaceutically acceptable carrier, ##STR00033## wherein, A is selected from the groups of (CH2)eN(R7)C(O)N(R8)(CH2)f or CH2C(O)NR7, wherein e and f are independently 0 or 1, wherein the left side of A is the side bonded to the ring substituted by R3 and R4; L is —[Cm(O)(Z)n(NH)q]-, wherein m, q are 0 or 1, n is 0-11, p is 0-8; Z is —C(R10).sub.2; R1 and R2 are independently selected from the group consisting of hydrogen, alkoxy, alkoxyalkoxy, alkoxyalkyl, alkyl, aryloxy, aryloxyalkyl, halo, haloalkoxy, haloalkyl, heterocycle, heterocyclylalkenyl, heterocyclylalkoxy, heterocyclylalkyl, heterocyclyloxyalkyl, hydroxy, hydroxyalkoxy, and hydroxyalkyl; R3 and R4 are independently selected from the group consisting of hydrogen, alkoxy, alkyl, halo, haloalkoxy, haloalkyl and hydroxy; R5 and R6 are independently selected from the group consisting of hydrogen, alkoxy, alkoxyalkyl, alkoxycarbonyl, alkyl, aryloxy, arylalkyl, carboxy, cyano, halo, haloalkoxy, haloalkyl, Hydroxy, hydroxyalkyl, nitro and —NRcRd; R7 and R8 are independently selected from the group consisting of hydrogen and alkyl; R9 is selected from the group consisting of hydrogen, hydroxy, amino, alkenyl, alkynyl, alkoxy, alkylamino, alkoxyalkyl, alkyl, alkoxycarbonyl, aryl, heterocycloalkyl; R10 is selected from the group consisting of hydrogen, alkyl, alkoxy, aryloxy, alkenyloxy, nitro, halo, primary, secondary and tertiary amine; Ra and Rb are independently selected from the group consisting of hydrogen, alkyl, alkylcarbonyl, alkylsulfonyl, arylsulfonyl, haloalkylsulfonyl and heterocyclylsulfonyl; Rc and Rd are independently selected from the group consisting of hydrogen, alkyl, alkylcarbonyl, aryl, arylalkyl, cycloalkyl, cycloalkylalkyl, heterocyclyl and heterocyclylalkyl.
2. The pharmaceutical composition according to claim 1, wherein said compound of Formula I is ##STR00034## ##STR00035## ##STR00036##
3. A method for preparing a compound of Formula I of claim 1 wherein R9 is hydroxy or a compound of Formula I of claim 2, comprising: Step (a), Reactant 1, is reacted with Reactant 2, in the presence of a catalyst and a condensing agent to obtain a protecting group-containing intermediate compound 1; ##STR00037## Step (b), the intermediate compound 1 is subjected to catalytic hydrogenation in a polar solvent to remove the protecting group to obtain an intermediate compound 2; ##STR00038## Step (c), the intermediate compound 2 and Linifanib are reacted in the presence of a catalyst and a condensing agent to obtain an intermediate compound 3; ##STR00039## Step (d), the intermediate compound 3 is subjected to acidic conditions to remove the protecting groups to obtain a compound of Formula I of claim 1 wherein R9 is hydroxy or a compound of Formula I of claim 2.
4. The method according to claim 3, wherein said step (a) is carried out at a reaction temperature of −20° C. to 125° C.; said catalyst is 1-hydroxybenzotriazole; said condensing agent is any one or more agents selected from 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, 1, 3-dicyclohexylcarbodiimide or 4-dimethylaminopyridine.
5. The method according to claim 3, wherein said step (b) is carried out at a reaction temperature of −20° C. to 125° C.; said catalyst is palladium on carbon or palladium hydroxide, dry or wet.
6. The method according to claim 3, wherein said step (c) is carried out at a reaction temperature of −20° C. to 125° C.; said catalyst is 1-hydroxybenzotriazole; and said condensing agent is any one or more agents selected from ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, 1, 3-dicyclohexylcarbodiimide or 4-dimethylaminopyridine.
7. The method according to claim 3, wherein said step (d) is carried out at a reaction temperature of −20° C. to 125° C.; and said acidic conditions is formic acid, acetic acid, or trifluoroacetic acid.
8. A method for treating a cancer in a subject, said method comprising: administering to a subject in need thereof the pharmaceutical composition according to claim 1 or 2; wherein said cancer is at least one selected from the group of: endometrial cancer, leukemia, colon cancer, breast cancer, liver cancer, lung cancer, prostate cancer, and renal cancer.
9. A method for treating liver cancer in a subject, said method comprising: administering to the subject in need thereof the pharmaceutical composition according to claim 1 or 2.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
(1)
(2)
(3)
(4)
(5)
EXAMPLES
Examples 1 and 2: Preparation of Target Compound 1
Example 1 Preparation of Intermediate Compound 3
(6) Weighed and took 600 mg of Linifanib (1.6 mmol), 324 mg of HOBT (2.4 mmol), and 460 mg of EDCl (2.4 mmol) to dissolve in 50 ml of dichloromethane, stirred and reacted for 0.5 h, controlling reaction temperature at 20.sup.˜40° C. and slowly added 912 mg of the intermediates compound 2 Asp (BOC)-Glu (OtBu)-(OtBu) (1.92 mmol) which is available in market, and finally added 516 mg of DIPEA (4.0 mmol); maintaining the reaction temperature and stirring reacting for 12 h. TLC (DCM/MeOH=40:1) detected a complete reaction. 100 ml of dichloromethane was added to dilute the reaction solution, and the solution was washed with 250 ml deionized water twice. The organic phase was then washed with 150 ml saturated sodium solution, and the organic phase was dried with anhydrous sodium sulfate. Desiccant was filtered and concentrated at low temperature to obtain a brown oily object. The oily object was carried out by Silica Gel column chromatography (DCM:MeOH=0:1.sup.˜100:1), and 716 mg of a white solid powder was obtain, with a yield rate of 53.8%.
Example 2 Preparation of Target Compound 1
(7) Weighed and took 500 mg of the intermediate Compound 3 (0.38 mmol) prepared in Example 1 to dissolve in 20 ml dichloromethane, the reaction temperature was controlled at −5.sup.˜5° C. Slowly added 3 ml of trifluoroacetate (0.04 mmol), and the reaction temperature was maintained and stir-reacted for 20.sup.˜24 h; TLC (DCM/MeOH=40:1) detected a complete reaction. Added 40 ml of dichloromethane to dilute to the reaction solution, washed two times with 120 ml of deionized water, then wash two times with 60 ml of 5% sodium bicarbonate solution, and then washed two times with 120 ml of deionized water. Organic phase was separated and dried with anhydrous sodium sulfate. Desiccant was filtered and concentrated at low temperature to obtain a reddish-brown oily object. The oily object was prepared by chromatographic separation, and 106 mg of a white solid powder was obtained with a yield rate of 44.9%. .sup.1HNMR (400 MHz, DMSO-d6) Delta: 9.37 (S, 1H), 8.67 (S, 1H), 8.32-8.27 (M, 2H), 7.99-7.96 (M, 1H), 7.65-7.59 (M, 3H), 7.42-7.39 (M, 2H), 7.27-7.18 (M, 2H), 7.13-7.08 (M, 1H), 6.81-6.78 (M, 2H), 5.25 (S, 2H), 4.34-4.11 (M, 2H), 3.71-3.60 (M, 1H), 2.91-2.81 (M, 1H), 2.37-2.31 (M, 2H), 2.28 (S, 3H), 2.24-2.20 (M, 1H), 1.89-1.86 (M, 1H). HPLC purity: 96.1% (214 nm), 95.5% (254 nm). MS (ESI): m/z 620.0 [M+1].sup.+
(8) Chemical Structure is:
(9) ##STR00020##
Examples 3-4: Preparation of Target Compound 2
Example 3 Preparation of Intermediate Compound 3
(10) Weighed and took 600 mg of Linifanib (1.6 mmol), 324 mg of HOBT (2.4 mmol) and 460 mg of EDCl (2.4 mmol) to dissolve in 50 ml of dichloromethane and stir-reacted for 0.5 h, and controlled the reaction temperature at 20.sup.˜40° C. slowly added 1978 mg of Intermediate compound 2 Asp(Boc)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-(OtBu) (1.92 mmol) which is available in market, and finally added 516 mg of DIPEA (4.0 mmol). The reaction temperature was maintained and stir-reacted for 12 h, and TLC (DCM/MeOH=40:1) detected the completion of reaction. The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution and separated. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=0:1 to 100:1) to give 1038 mg of white solid powder, and the yield rate was 46.8%.
Example 4 Preparation of Target Compound 2
(11) The intermediate compound prepared in Example 3 was weighed and took 527 mg (0.38 mmol) to dissolve in 20 ml of dichloromethane, and the reaction temperature was controlled at −5 to 5° C., and 3 ml (0.04 mmol) of trifluoroacetic acid was slowly added to maintain the reaction temperature. The reaction was stirred for 20-24 h, and the reaction was complete by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 40 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated, and the organic phase was dried over anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was subjected to chromatography to give 166 mg of white solid powder, and the yield rate as 43.5%. HPLC purity: 95.9% (214 nm), 96.5% (254 nm). MS (ESI): m/z 1007.0 [M+1].sup.+
(12) The chemical structure is:
(13) ##STR00021##
Examples 5-6: Preparation of Target Compound 3
Example 5 Preparation of Intermediate Compound 3
(14) Weighed 600 mg of Linifanib (1.6 mmol), 324 mg of HOBT (2.4 mmol) and 460 mg of EDCl (2.4 mmol) to dissolve in 50 ml of dichloromethane, stir-reacted for 0.5 h, and controlled the reaction temperature at 20.sup.˜40° C. Slowly added 3,756 mg of intermediate compound 2 Asp(Boc)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu (OtBu)-(OtBu) (1.92 mmol) which is available in market, and finally DIPEA 516 mg (4.0 mmol) was added, and the reaction was stirred for 12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution and separated. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=0:1 to 100:1) to give 1051 mg of white solid powder, with a yield rate of 28.4%.
Example 6 Preparation of Target Compound 3
(15) 879 mg of the intermediate compound 3 prepared in Example 5 was weighed (0.38 mmol) and dissolved in 20 ml of dichloromethane, and the reaction temperature was controlled at −5 to 5° C. 3 ml of trifluoroacetic acid (0.04 mmol) was slowly added to maintain the reaction temperature. The reaction was stirred for 20-24 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 40 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated, and the organic phase was dried over anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was subjected to chromatography to give 174 mg of a white solid powder, the yield rate was 27.7%. HPLC purity: 92.5% (214 nm), 94.1% (254 nm). MS (ESI): m/z 1652.0 [M+1].sup.+
(16) Chemical structure is:
(17) ##STR00022##
Examples 7-10: Preparation of Target Compound 4
Example 7 Preparation of Intermediate Compound 1
(18) Weighed 304 mg of benzyl-(6-amino)hexaneate hydrochloride (1.18 mmol), 238 mg of HOBT (1.76 mmol) and 192 mg of MCI 192 (1.76 mmol) to dissolve in 50 ml of dichloromethane, stirred at room temperature. Controlled reaction temperature at 20.sup.˜40° C. Slowly added 584 mg of Asp(Boc)-Glu(OtBu)-(OtBu) (1.23 mmol), maintained the reaction temperature, stir-reacted for 4 h, TLC (DCM/MeOH=40:1)) detected the completion of the reaction. The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution and separated. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (petroleum ether/acetone=10:1 to 2:1) to yield 338 mg of a yellow solid powder; the yield rate was 42.3%.
Example 8 Preparation of Intermediate Compound 2
(19) Weighed 285 mg of the intermediate compound 1 (0.42 mmol) prepared in Example 7 and dissolved in 60 ml of anhydrous methanol, and 10% Pd/C 25 mg was added under nitrogen atmosphere, and hydrogen was introduced and exchanged three times. The reaction was controlled at 2 MPa in the atmosphere, and the reaction was carried out at 20-65° C. for 6-12 h. The reaction was complete by TLC (DCM/MeOH=40:1). The reaction solution was filtered under nitrogen atmosphere to recover palladium carbon. The filtrate was concentrated at low temperature to give a yellow brown oily object. The oily object was subjected to chromatography to give 115 mg of pale-yellow solid powder, and yield rate was 46.6%.
Example 9 Preparation of Intermediate Compound 3
(20) Weighed 600 mg of Linifanib (1.6 mmol), 324 mg of HOBT (2.4 mmol) and 460 mg of EDCl (2.4 mmol) to dissolve in 50 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature 20.sup.˜40° C. Slowly added 1129 mg of intermediate compound 2 (1.92 mmol) prepared in example 8 and finally DIPEA 516 mg (4.0 mmol) was added, and the reaction was stirred for 12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution, separated and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=0:1 to 100:1) to give 776 mg of a white powder, and the yield rate was 51.3%.
Example 10 Preparation of Target Compound 4
(21) Weighed 595 mg of the intermediate compound 3 (0.63 mmol) prepared in Example 9 to dissolve in 20 ml of dichloromethane, controlled the reaction temperature at −5.sup.˜5° C. Slowly added 3 ml (0.04 mmol) of trifluoroacetic acid, maintained the reaction temperature. The reaction was stirred for 20-24 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 40 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated, and the organic phase was dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was subjected to chromatography to give 193 mg of a white solid powder, yield rate was 41.9%. .sup.1HNMR (400 MHz, DMSO-d6) δ: 9.30 (s, 1H), 8.67 (d, J=7.6 Hz, 1H), 8.58 (d, J=2.0 Hz, 1H) 8.31 (d, J=8.0 Hz, 1H) 8.25-8.22 (m, 1H), 8.09 (s, 3H), 7.99 (d, J=6.4 Hz, 1H), 7.64-7.58 (m, 3H), 7.41 (d, J=8.4 Hz, 1H), 7.19-7.09 (m, 2H), 6.83 (d, J=5.6 Hz, 1H), 5.17 (s, 2H), 4.26-4.14 (m, 2H), 3.15-2.97 (m, 4H), 2.70-2.54 (m, 2H), 2.33-2.32 (m, 2H), 2.28 (s, 3H), 2.00-1.69 (m, 4H), 1.50-1.36 (m, 4H). HPLC purity: 98.2% (214 nm), 98.5% (254 nm). MS (HI): m/z 733.0 [M+1].sup.+
(22) The chemical structure is:
(23) ##STR00023##
Examples 11-14: Preparation of Target Compound 5
Example 11 Preparation of Intermediate Compound 1
(24) Weighed 304 mg of benzyl-(6-amino)hexaneate hydrochloride (1.18 mmol), 238 mg of HOBT (1.76 mmol) and 192 mg of MCI (1.76 mmol) to dissolve in 50 ml of dichloromethane, stirred at room temperature. Controlled reaction temperature at 20.sup.˜40° C. Slowly added 1267 mg of Asp(Boc)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-(OtBu) (1.23 mmol), and maintained the reaction temperature and stir-reacted for 4 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution and separated. The organic phase was dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oil was subjected to silica gel column chromatography (peel ether/acetone=10:1 to 2:1) to yield 544 mg of a yellow solid powder. The yield rate was 37.4%.
Example 12 Preparation of Intermediate Compound 2
(25) 518 mg of the intermediate compound 1 (0.42 mmol) prepared in Example 11 was weighed and dissolved in 60 ml of anhydrous methanol, and 10% Pd/C 25 mg was added under nitrogen atmosphere, and hydrogen was introduced and exchanged for 3 times. The reaction was controlled at 2 MPa in the atmosphere, and the reaction was carried out at 20-65° C. for 6-12 h. The reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was filtered under nitrogen atmosphere to recover palladium carbon. The filtrate was concentrated at low temperature to give a yellow-brown oily object. The oily object was subjected to chromatography to give 244 mg of a pale-yellow solid powder, yield rate was 50.8%.
Example 13 Preparation of Intermediate Compound 3
(26) Weighed 600 mg of Linifanib 600 (1.6 mmol), 324 mg of HOBT (2.4 mmol) and 460 mg of EDCl (2.4 mmol) to dissolve in 50 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature at 20.sup.˜40° C. Slowly added 2195 mg of the intermediate compound 2 (1.92 mmol) prepared in example 12, and finally DIPEA 516 mg (4.0 mmol) was added, the reaction was stirred for 12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution, separated and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=0:1 to 100:1) to yield 1054 mg of a white solid powder, the yield rate was 43.9%.
Example 14 Preparation of Target Compound 5
(27) Weighed 945 mg of the intermediate compound 3 (0.63 mmol) prepared in Example 13 and dissolve it in 20 ml of dichloromethane. Controlled the reaction temperature at −5.sup.˜5° C. Slowly added 3 ml (0.04 mmol) of trifluoroacetic acid to maintain the reaction temperature. The reaction was stirred for 20-24 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 40 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated, and the organic phase was dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was subjected to chromatography to give 273 mg of a white solid powder, yield rate was 38.7%. HPLC purity: 97.2% (214 nm), 98.4% (254 nm). MS (ESI): m/z 1120.0 [M+1].sup.+
(28) The chemical structure is:
(29) ##STR00024##
Examples 15-18: Preparation of Target Compound 6
Example 15 Preparation of Intermediate Compound 1
(30) Weighed 304 mg of benzyl-(6-amino)hexaneate hydrochloride (1.18 mmol), 238 mg of HOBT (1.76 mmol), 192 mg of MCI (1.76 mmol) to dissolve in 50 ml of dichloromethane, stirred at room temperature. Controlled reaction temperature at 20˜40° C. Slowly added 2406 mg of Asp(Boc)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu (OtBu)-Glu(OtBu)-(OtBu) (1.23 mmol), maintained the reaction temperature and the reaction was stirred for 4 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml saturated sodium solution and separated. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (petrol ether/acetone=10:1 to 2:1) to give 724 mg of a yellow solid powder, the yield rate was 28.4%.
Example 16 Preparation of Intermediate Compound 2
(31) 907 mg of the intermediate compound 1 (0.42 mmol) prepared in Example 15 was weighed and dissolved in 60 ml of anhydrous methanol, and 10% Pd/C 25 mg was added under nitrogen atmosphere, and hydrogen was introduced and exchanged three times. The reaction was controlled at 2 MPa in the atmosphere, and the reaction was carried out at 20-65° C. for 6-12 h. The reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was filtered under nitrogen atmosphere to recover palladium carbon. The filtrate was concentrated at low temperature to give a yellow brown oily object. The oily object was subjected to chromatography to give 357 mg of a pale yellow solid powder, the yield rate was 41.1%.
Example 17 Preparation of Intermediate Compound 3
(32) Weighed 600 mg of Linifanib (1.6 mmol), 324 mg of HOBT (2.4 mmol) and 460 mg of EDCl (2.4 mmol) to dissolve in 250 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature at 20˜40° C. Slowly added 3972 mg of the intermediate compound 2 (1.92 mmol) prepared in example 16, and finally DIPEA 516 mg (4.0 mmol) was added, the reaction was stirred for 12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution, separated, and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oil object was subjected to silica gel column chromatography (DCM:MeOH=0:1 to 100:1) to give 1265 mg of a white solid powder; the yield rate was 32.6%.
Example 18 Preparation of Target Compound 17
(33) 1528 mg of the intermediate compound 3 (0.63 mmol) prepared in Example 17 was weighed and dissolved in 60 ml of dichloromethane, and the reaction temperature was −5 to 5° C., and 3 ml (0.04 mmol) of trifluoroacetic acid was slowly added to maintain the reaction temperature. The reaction was stirred for 20-24 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 40 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was subjected to chromatography to give 307 mg of a white solid powder, the yield rate was 27.6%. HPLC purity: 96.5% (214 nm), 97.7% (254 nm). MS (ESI): m/z 1765.0 [M+1].sup.+
(34) The chemical structure is:
(35) ##STR00025##
Examples 19-22: Preparation of Target Compound 7
Example 19 Preparation of Intermediate Compound 1
(36) 404 mg of Benzyl-(12-amino)dodecanoate hydrochloride (1.18 mmol), 238 mg of HOBT (1.76 mmol), and 192 mg of MCI (1.76 mmol) were weighed and dissolved in 50 ml of dichloromethane, stirred at room temperature. Controlled reaction temperature at 20.sup.˜40° C. Slowly added 584 mg of Asp(Boc)-Glu(OtBu)-(OtBu) (1.23 mmol), maintained the reaction temperature, stirred the reaction for 4 h. The reaction was detected to be complete by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution, separated, and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. This oily object was subjected to silica gel column chromatography (petroleum ether/acetone=10:1 to 2:1) to yield 278 mg of a yellow solid powder. The yield rate was 35.6%.
Example 20 Preparation of Intermediate Compound 2
(37) 2270 mg of the intermediate compound 1 (3.43 mmol) prepared in Example 19 was weighed and dissolved in 100 ml of anhydrous methanol, 10% Pd/C 50 mg was added under nitrogen atmosphere, and hydrogen was introduced and exchanged for 3 times. The reaction was controlled at 2 MPa in the atmosphere, and the reaction was carried out at 20-65° C. for 6-12 h. The reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was filtered under nitrogen atmosphere to recover palladium carbon. The filtrate was concentrated at low temperature to give a yellow brown oily object. The oily object was subjected to chromatography to give 1101 mg of a pale-yellow solid powder, and yield rate was 56.1%.
Example 21 Preparation of Intermediate Compound 3
(38) Weighed 600 mg of Linifanib (1.6 mmol), 324 mg of HOBT (2.4 mmol), 460 mg of EDCl (2.4 mmol) in 50 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature at 20.sup.˜40° C. Slowly added 1098 mg of the intermediate compound 2 (1.92 mmol) prepared in example 4420 and finally DIPEA 516 mg (4.0 mmol) was added and the reaction was stirred for 12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution, separated, and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=1:1 to 100:1) to give 589 mg of a white powder, and the yield rate was 39.6%.
Example 22 Preparation of Target Compound 7
(39) Weighed 585 mg of the intermediate compound 3 (0.63 mmol) prepared in Example 21 and dissolve it in 20 ml of dichloromethane. Controlled the reaction temperature at −5.sup.˜5° C. Slowly add 3 ml (0.04 mmol) of trifluoroacetic acid to maintain the reaction temperature. The reaction was stirred for 20-24 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 40 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated, and the organic phase dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was subjected to chromatography to give 223 mg of a white solid powder, yield rate was 43.3%. .sup.1HNMR (400 MHz, DMSO-d6) δ: 9.31 (s, 1H), 8.67 (d, J=7.6 Hz, 1H) 8.59 (s, 1H), 8.31 (d, J=8.4 Hz, 1H), 8.21 (s, 1H), 8.19-7.98 (m, 4H), 7.64-7.57 (m, 3H), 7.42 (d, J=8.0 Hz, 2H), 7.19-7.09 (m, 2H), 6.83-6.82 (m, 1H), 5.18 (s, 2H), 4.29-4.13 (m, 2H), 3.12-2.96 (m, 4H), 2.73-2.68 (m, 2H), 2.67-2.63 (m, 2H), 2.28 (s, 3H), 2.12-1.67 (m, 4H), 1.37-1.23 (m, 18H). HPLC purity: 99.0% (214 nm), 99.0% (254 nm). MS (ESI): m/z 817.1 [M+1].sup.+
(40) The chemical structure is:
(41) ##STR00026##
Examples 23-26: Preparation of Target Compound 8 (Linifanib-C.SUB.12.-AA.SUB.5.)
Example 23 Preparation of Intermediate Compound 1
(42) 404 mg of Benzyl-(12-amino)dodecanoate hydrochloride (1.18 mmol), 238 mg of HOBT (1.76 mmol), and 192 mg of EDCl (1.76 mmol) were weighed and dissolved in 250 ml of dichloromethane, stirred at room temperature. Controlled reaction temperature at 20.sup.˜40° C. Slowly added 1267 mg of Asp(Boc)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-(OtBu) (1.23 mmol), and maintained the reaction temperature. Stir-reacted for 4 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml saturated sodium solution, separated, and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (petroleum ether/acetone=10:1 to 2:1) to give 553 mg of a yellow solid powder, the yield rate was 35.6%.
Example 24 Preparation of Intermediate Compound 2
(43) Weighed 4000 mg (3.0 mmol) of the intermediate compound 1 prepared in Example 23, dissolved in 100 ml of anhydrous methanol, 10% Pd/C 50 mg under nitrogen atmosphere, and replaced with hydrogen for 3 times. The reaction was controlled at 2 MPa in the atmosphere, and the reaction was carried out at 20-65° C. for 6-12 h. The reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was filtered under nitrogen atmosphere to recover palladium carbon. The filtrate was concentrated at low temperature to give a yellow brown oily object. The oily object was subjected to chromatography to give 1595 mg of a pale-yellow solid powder, the yield rate was 42.8%. .sup.1HNMR (CDCl.sub.3) δ1.27 (brs, 14H), 1.46.sup.˜1.47 (m, 54H), 1.65.sup.˜1.85 (m, 8H), 2.34.sup.˜2.35 (brs, 16H), 3.06.sup.˜3.36 (brs, 2H), 4.46-4.52 (m, 5H), 6.31 (brs, 1H, —NH—C═O), 6.68 (brs, 1H, —NH—C═O), 6.91 (brs, 2H, —NH—C═O), 7.19 (brs, 1H, —NH—C═O), 7.54 (brs, 1H, —NH—C═O). .sup.13CNMR (CDCl.sub.3) δ 192.97, 190.34, 173.02, 172.22, 172.00, 171.81, 171.22, 171.08, 170.76, 82.42, 82.27, 82.08, 82.02, 80.64, 80.53, 52.35, 51.83, 51.44, 39.84, 33.79, 32.52, 32.15, 31.61, 31.11, 29.26, 29.11, 28.97, 28.92, 28.86, 28.78, 28.71, 28.48, 28.33, 28.10, 28.01, 27.98, 27.76, 27.65, 26.68, 24.61, 12.10.
(44) The chemical structure is:
(45) ##STR00027##
Example 25 Preparation of Intermediate Compound 3
(46) Weighed 600 mg of Linifanib (1.6 mmol), 324 mg of HOBT (2.4 mmol), and 460 mg of EDCl (2.4 mmol) to dissolve in 250 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature at 20.sup.˜40° C. Slowly added 2340 mg (1.9 mmol) of the intermediate compound 2 prepared in example 24 and finally DIPEA 516 mg (4.0 mmol) was added, the reaction was stirred for 12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution, separated, and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=0:1 to 100:1) to give 1132 mg of a white solid powder; the yield rate was 44.7%.
Example 26 Preparation of Target Compound 8 (Linifanib-C.SUB.12.-AA.SUB.5.)
(47) Weighed 1000 mg (0.63 mmol) of the intermediate compound 3 prepared in Example 25 and dissolved it in 60 ml of dichloromethane, and slowly added 3 ml (0.04 mmol) of trifluoroacetic acid at a reaction temperature of −5 to 5° C. to maintain the reaction temperature. The reaction was stirred for 20-24 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 40 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated, and the organic phase dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was subjected to chromatography to give 293 mg of a white solid powder, yield rate was 38.6%. .sup.1HNMR (400 MHz, DMSO-d6) δ: 9.51 (s, 1H), 8.97 (s, 1H), 8.56 (s, 1H), 8.32 (d, J=8.4 Hz, 1H), 8.18 (s, 1H), 8.02-7.96 (m, 4H), 7.66-7.57 (m, 3H), 7.42 (d, J=8.8 Hz, 2H), 7.19-7.08 (m, 2H), 6.82-6.81 (m, 1H), 5.18 (s, 2H), 4.16-3.98 (m, 6H), 3.07-2.96 (m, 4H), 2.67-2.63 (m, 2H), 2.28 (s, 3H), 2.24-2.14 (m, 8H), 2.03-1.87 (m, 5H), 1.77-1.67 (m, 4H), 1.37-1.23 (m, 18H). HPLC purity: 99.3% (214 nm), 99.1% (254 nm). MS (HI): m/z 1204.5 [M+1].sup.+
(48) The chemical structure is:
(49) ##STR00028##
Examples 27-30: Preparation of Target Compound 9
Example 27 Preparation of Intermediate Compound 1
(50) 404 mg (1.18 mmol) of Benzyl-(12-amino)dodecanoate hydrochloride, 238 mg (1.76 mmol) of HOBT, and 192 mg (1.76 mmol) of MCI were weight and dissolved in 250 ml of dichloromethane, stirred at room temperature. Controlled reaction temperature at 20.sup.˜40° C. Slowly added 2406 mg (1.23 mmol) of Asp(Boc)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu(OtBu)-Glu (OtBu)-Glu(OtBu)-(OtBu), maintained the reaction temperature and stir-reacted for 4 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml saturated sodium solution, separated, and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. This oily object was subjected to silica gel column chromatography (petroleum ether/acetone=10:1 to 2:1) to yield 627 mg of a yellow solid powder, yield rate was 23.7%.
Example 28 Preparation of Intermediate Compound 2
(51) 6732 mg (3.0 mmol) of the intermediate compound 1 prepared in Example 27 was weighed and dissolved in 200 ml of anhydrous methanol, 10% Pd/C 50 mg was added under nitrogen atmosphere, and hydrogen was introduced and exchanged for 3 times. The reaction was controlled at 2 MPa in the atmosphere, and the reaction was carried out at 20-65° C. for 6-12 h. The reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was filtered under nitrogen atmosphere to recover palladium carbon. The filtrate was concentrated at low temperature to give a yellow brown oily object. The oily object was subjected to chromatography to give 2480 mg of a pale-yellow solid powder, yield rate was 38.4%.
Example 29 Preparation of Intermediate Compound 3
(52) Weighed 600 mg (1.6 mmol) of Linifanib, 324 mg (2.4 mmol) of HOBT and 460 mg (2.4 mmol) of EDCl, and dissolved in 250 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature at 20.sup.˜40° C. Slowly added 4134 mg (1.92 mmol) of intermediate compound 2 prepared in example 28 and finally DIPEA 516 mg (4.0 mmol) was added, the reaction was stirred for 12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 100 ml of dichloromethane, washed twice with 250 ml of deionized water, and the organic phase was separated. The organic phase was washed with 150 ml of saturated sodium solution, separated, and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=1:1 to 100:1) to give 1152 mg of a white solid powder, and the yield rate was 28.7%.
Example 30 Preparation of Target Compound 9
(53) Weighed 1581 mg (0.63 mmol) of the intermediate compound 3 prepared in Example 29, dissolved in 60 ml of dichloromethane, and slowly added 3 ml (0.04 mmol) of trifluoroacetic acid at a reaction temperature of −5 to 5° C. to maintain the reaction temperature. The reaction was stirred for 20-24 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 40 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated, and the organic phase dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was subjected to chromatography to give 274 mg of a white solid powder, yield rate was 23.5%. HPLC purity: 93.2% (214 nm), 94.5% (254 nm). MS (ESI): m/z 1849.7 [M+1].sup.+
(54) Chemical structure is:
(55) ##STR00029##
Experiments 31-33: Preparation of Target Compound 10
Example 31 Preparation of Metabolite Intermediate Compound Mc
(56) Weighed 137 mg (0.42 mmol) of Boc-L-aspartic acid 1-benzyl ester, 77.8 mg (0.58 mmol) of HOBT, and 110 mg (0.58 mmol) of EDCl and dissolved in 10 ml of dichloromethane, stirred the reaction for 0.5 h, controlled a reaction temperature of 20 to 40° C. 143 mg (0.38 mmol) of Linifanib was slowly added and finally DIPEA 124 mg (0.96 mmol) was added. After the addition, the reaction temperature was maintained for 4 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction mixture was diluted with 50 ml of methylene chloride and washed twice with 100 ml of deionized water. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=1:1 to 30:1) to give 221 mg of a yellow oily object, the yield rate was 77.3%.
Example 32 Preparation of Metabolite Intermediate Compound Md
(57) 198 mg (0.29 mmol) of the intermediate compound Mc prepared in Example 31 was weighed and dissolved in 20 ml of dichloromethane, and the reaction temperature was −5 to 5° C., and 3 ml (0.04 mmol) of trifluoroacetic acid was slowly added to maintain the reaction temperature. The reaction was stirred for 1.5 to 2 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 50 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated to a yellow oily object. The oily object was chromatographed to give 131 mg of a yellow oily object, the yield rate was 77.8%.
Example 33 Preparation of Target Compound 10
(58) 336 mg (0.58 mmol) of the intermediate Md prepared in Example 32 was weighed and dissolved in 30 ml of anhydrous methanol, and 10% Pd/C 25 mg was added under nitrogen atmosphere, and hydrogen was introduced and exchanged for 3 times. The reaction was controlled at 2 MPa in the atmosphere, the reaction was carried out at 20.sup.˜65° C. for 6.sup.˜12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was filtered under nitrogen atmosphere to recover palladium carbon. The filtrate was concentrated at low temperature to give a pale-yellow oily object. The oily object was subjected to chromatography to give 178 mg of a white solid powder, yield rate was 62.6%. HPLC purity: 97.2% (214 nm), 98.6% (254 nm). MS (ESI): m/z 491.0[M+1].sup.+
(59) The chemical structure is:
(60) ##STR00030##
Examples 34-38: Preparation of Target Compound 11
Example 34 Preparation of Metabolite Intermediate Compound Ma
(61) 136 mg (0.59 mmol) of 6-(BOC-amino)hexane acid, 107 mg (0.8 mmol) of HOBT, and 152 mg (0.8 mmol) of EDCl were weighed and dissolved in 10 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature at 20.sup.˜40° C. 200 mg (0.53 mmol) of Linifanib was slowly added and finally DIPEA 171 mg (1.3 mmol) was added. After the addition, the reaction temperature was maintained and stir-reacted for 4 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction mixture was diluted with 50 ml of methylene chloride and washed twice with 100 ml of deionized water, and the organic phase was separated and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=1:1 to 30:1) to give 207 mg of a yellow oily object, and the yield rate was 59.8%.
Example 35 Preparation of Metabolite Intermediate Compound Mb
(62) 194 mg (0.33 mmol) of the intermediate Ma prepared in Example 34 was weighed and dissolved in 20 ml of dichloromethane, and the reaction temperature was a −5 to 5° C., and 3 ml (0.04 mmol) of trifluoroacetic acid was slowly added; the reaction temperature was maintained and stir-reacted for 1.5.sup.˜2 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 50 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was subjected to chromatography to give 147 mg of a yellow oily object, the yield rate was 91.1%.
Example 36 Preparation of Metabolite Intermediate Compound Mc
(63) 137 mg (0.42 mmol) of Boc-L-aspartic acid 1-benzyl ester, 77.8 mg (0.58 mmol) of HOBT and 110 mg (0.58 mmol) EDCl were weighed and dissolved in 10 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature at 20 to 40° C. 185 mg (0.38 mmol) of the intermediate Mb prepared in Example 35 was slowly added, and finally DIPEA 124 mg (0.96 mmol) was added. After the addition, the reaction temperature was maintained and stir-reacted for 4 h, the reaction was completed by TLC (DCM/MeOH=40:1). The reaction mixture was diluted with 50 ml of methylene chloride and washed twice with 100 ml of deionized water. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=1:1 to 30:1) to give 200 mg of a yellow oily object, the yield rate was 66.3%.
Example 37 Preparation of Metabolite Intermediate Compound Md
(64) 230 mg (0.29 mmol) of the intermediate compound Mc prepared in Example 36 was weighed and dissolved in 20 ml of dichloromethane, and the reaction temperature was controlled at −5 to 5° C. Slowly add 3 ml (0.04 mmol) of trifluoroacetic acid to maintain the reaction temperature. The reaction was stirred for 1.5 to 2 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 50 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated to a yellow oily object. The oily object was subjected to chromatography to give 158 mg of a yellow oily object, the yield rate was 78.4%.
Example 38 Preparation of Target Compound 11
(65) 425 mg (0.61 mmol) of the intermediate Md prepared in Example 37 was weighed and dissolved in 30 ml of anhydrous methanol, and 10% Pd/C 25 mg was added under nitrogen atmosphere, and hydrogen was introduced and exchanged for 3 times. The reaction was controlled at 2 MPa in the atmosphere, the reaction was carried out at 20.sup.˜65° C. for 6.sup.˜12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was filtered under nitrogen atmosphere to recover palladium carbon. The filtrate was concentrated at low temperature to give a pale-yellow oily object. The oily object was subjected to p chromatography to give 234 mg of a white solid powder, and the yield rate was 63.4%. HPLC purity: 96.2% (214 nm), 98.1% (254 nm). MS (ESI): m/z 604.3[M+1].sup.+
(66) The chemical structure is:
(67) ##STR00031##
Examples 39-43: Preparation of Target Compound 12 (Linifanib-C12-Asp)
Example 39 Preparation of Metabolite Intermediate Compound Ma
(68) 186 mg (0.59 mmol) of 12-(BOC-amino)dodecanoic acid, 107 mg (0.8 mmol) of HOBT and 152 mg (0.8 mmol) of EDCl were weighed and dissolved in 10 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature at 20.sup.˜40° C. Slowly added 200 mg (0.53 mmol) of Linifanib and finally added DIPEA 171 mg (1.3 mmol). The reaction temperature was maintained and stir-reacted for 4 hr. The reaction was completed by TLC (DCM/MeOH=40:1). The reaction mixture was diluted with 50 ml of methylene chloride and washed twice with 100 ml of deionized water. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=1:1 to 30:1) to give 225 mg of a yellow oily object, and the yield rate was 63.2%.
Example 40 Preparation of Metabolite Intermediate Compound Mb
(69) 225 mg (0.33 mmol) of the intermediate Ma prepared in Example 39 was weighed and dissolved in 20 ml of dichloromethane, and the reaction temperature was −5 to 5° C. 3 ml (0.04 mmol) of trifluoroacetic acid was slowly added to maintain the reaction temperature. Stir-reacted for 1.5.sup.˜2 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 50 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a red brown oily object. The oily object was chromatographed to give 220 mg of a yellow oily object, the yield rate was 95%.
Example 41 Preparation of Metabolite Intermediate Compound Mc
(70) 137 mg (0.42 mmol) of Boc-L-aspartic acid 1-benzyl ester, 77.8 mg (0.58 mmol) of HOBT, and 110 mg (0.58 mmol) of EDCl were weighed and dissolved in 10 ml of dichloromethane, stirred the reaction for 0.5 h, controlled the reaction temperature of 20 to 40° C. 220 mg (0.38 mmol) of the intermediate Mb prepared in Example 40 was slowly added at finally DIPEA 124 mg (0.96 mmol) was added. The reaction was stirred for 4 h and completed by TLC (DCM/MeOH=40:1). The reaction mixture was diluted with 50 ml of methylene chloride and washed twice with 100 ml of deionized water. The desiccant was filtered off and the filtrate was concentrated at low temperature to give a brown oily object. The oily object was subjected to silica gel column chromatography (DCM:MeOH=1:0-30:1) to give 250 mg of a yellow oily object, the yield rate was 74.2%.
Example 42 Preparation of Metabolite Intermediate Compound Md
(71) 250 mg (0.29 mmol) of the intermediate compound Mc prepared in Example 41 was weighed and dissolved in 20 ml of dichloromethane, and slowly added 3 ml (0.04 mmol) of trifluoroacetic acid at a reaction temperature of −5 to 5° C. to maintain the reaction temperature. The reaction was stirred for 1.5 to 2 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was diluted with 50 ml of dichloromethane, washed twice with 120 ml of deionized water, twice with 60 ml of 5% sodium hydrogen carbonate solution, and twice with 120 ml of deionized water. The organic phase was separated and dried with anhydrous sodium sulfate. The desiccant was filtered off and the filtrate was concentrated to a yellow oily object. The oily object was subjected to chromatography to give 158 mg of a yellow oily object, the yield rate was 71%.
Example 43 Preparation of Target Compound 12 (Linifanib-C.SUB.12.-Asp)
(72) 210 mg of the intermediate Md prepared in Example 42 was weighed and dissolved in 30 ml of anhydrous methanol, added 10% Pd/C 25 mg under nitrogen protection, hydrogen was introduced and exchanged for three times, and the reaction was controlled at 2 MPa in the atmosphere. The reaction was carried out at 20.sup.˜65° C. for 6.sup.˜12 h, and the reaction was completed by TLC (DCM/MeOH=40:1). The reaction solution was filtered under nitrogen atmosphere to recover palladium carbon. The filtrate was concentrated at low temperature to give a pale-yellow oily object. The oily object was subjected to chromatography to give 102 mg of a white solid powder, and the yield rate was 54.8%. .sup.1HNMR (DMSO) δ: 1.22 (m, 12H), 1.35 (m, 4H), 1.71 (s, 2H), 2.41 (s, 3H, —CH3), 2.64 (m, 1H), 2.72 (m, 1H), 3.34 (m, 6H), 3.50 (m, 2H), 5.20 (s, 2H), 6.83 (brs, 1H), 7.10 (m, 1H), 7.18 (m, 1H), 7.40 (d, J=7.5 Hz, 2H), 7.58 (m, 1H), 7.67 (d, J=7.5 Hz, 2H), 7.91 (m, 1H), 8.23 (m, 1H), 8.32 (m, 1H), 9.01 (m, 1H), 9.83 (m, 1H). HPLC purity: 98.5% (214 nm), 99.3% (254 nm). MS (ESI): m/z 688.4[M+1].sup.+
(73) The chemical structure is:
(74) ##STR00032##
Example 44 Effect of Linifanib Related Compounds on the Proliferation of Tumor Cell Lines
(75) This application measured the half-inhibitory concentration (IC50 value) of 13 compounds (Compound 1-12 and Linifanib on 54 commercial tumor cell lines (including 26 liver cancer cell lines) by cell proliferation assay (Alamar Blue assay platform). The difference between the 12 compounds and the active drug Linifanib activity was compared.
(76) 1. Instruments and Materials Thermo 311 CO.sub.2 incubator; Haier biosafety cabinet; Molecular Devices microplate reader; Xiangyi brand L530 desktop low speed centrifuge; Olympus IX51 inverted fluorescence microscope, DMEM, RPMI 1640, MEM, DMEM/F12 1:1 medium, Fetal bovine serum, 0.25% trypsin solution, phosphate buffer (Thermo Fisher Shanghai Co., Ltd.); sigma dimethyl sulfoxide (DMSO), resazurin; 54 commercial tumor cell lines (including 26 liver cancer cell lines). Experimental drugs: compounds 1-12 and active drug Linifanib; chemotherapeutic drug Doxorubicin (HY-15142; Shanghai Qianyuan Biomedical Technology Co., Ltd.).
(77) 2. Experimental Methods 2.1 Cultivation of different cell lines 54 cell lines were cultured in a culture medium containing fetal bovine serum and placed in a 5% CO 2 incubator at 37° C. for incubation. The cells were all grown in an adherent state, and the growth was observed under an inverted microscope, and subculture was performed when the cell confluence rate reached 80%-90%. The proportion and quantity of passage were determined by experimental needs. The ratio of subculture of this cell line was generally 1:2.sup.˜1:3. 2.2 inhibition effect on the proliferation of different tumor cell lines Cell test: 54 cell lines in logarithmic growth phase were inoculated in 96-well culture plates at 500.sup.˜1×104/well (the optimal seeding density of each cell line was determined in pre-experiment), After incubating at 37° C. for 4 h in a 5% CO.sub.2 humidification incubator, added 10 μL of compounds 1-12 or Linifanib to each well, and tested 9 drug concentration gradients for each compound (diluted from the highest concentration of the test by 3.16 times). The solubility of each compound was different at a starting concentration of 30 or 100 μM, respectively. The QC reference compound Doxorubicin was added simultaneously to each cell line test, and the final drug concentrations were 10, 3.16, 1, 0.31, 0.1, 0.03, 0.01, 0.003, and 0.001 μM, respectively. In addition, a positive control group (100% inhibition) and a negative control group (0% inhibition) were set at the same time. The drug group was repeated for 2 wells per concentration, and the positive control group and the negative control group were repeated for 6 wells. After the culture was continued for 6 days in the incubator, AlamarBlue test operation was followed; AlamarBlue test procedure: Incubated with 10 μL of AlamarBlue reagent per well for 1-4 h, shook for 1-2 min, MD microplate reader EX: 560 nm, EM: 590 nm wavelength to measure fluorescence, recorded the results, calculated the cell inhibition rate of the compound of the invention. Cell inhibition rate (%)=(A0% inhibition−A administration)/(A0% inhibition−A100% inhibition)×100%, and then using the method of nonlinear regression using Graph Pad Prism 5.0 or MATILAB software (usually using four parameters) to graph and obtain a drug dose response curve to obtain an IC50 value of the compound of the present invention acting on a cancer cell line.
(78) 3. Results and Analysis 3.1 The IC50 summary results of 13 test samples (compounds 1-12 and Linifanib) on 54 commercial tumor cell lines are shown in Table 1.
(79) TABLE-US-00003 TABLE 1 Summary of IC50 (μM) values of six compounds against 54 commercial tumor cell lines Tumor Tumor Compound 8 Compound 12 cell line cell line (Linifanib- (Linifanib- number name C.sub.12-AA.sub.5) C.sub.12-Asp) Compound 1 Compound 4 Compound 7 Linifanib 1 22RV1 >100 >30 6.69 >100 >100 7.50 2 AN3CA 3.14 2.08 0.31 28.65 2.94 0.19 3 CCRF- >100 >30 2.50 51.66 14.96 5.03 CEM 4 DLD1 51.06 >30 9.86 >100 96.96 11.59 5 DU145 20.69 >30 2.76 37.98 17.19 5.01 6 HCCLM3 >100 >30 11.43 82.04 >100 6.67 7 HT1080 45.26 >30 13.19 >100 >100 6.40 8 HT55 >100 >30 16.75 >100 >100 22.29 9 HuTu80 43.78 >30 3.17 38.25 21.05 1.85 10 K562 >100 >30 3.99 44.16 23.95 10.85 11 KASUMI-1 0.10 0.05 0.051 1.713 0.096 0.01 12 KM12 12.38 >30 1.89 33.88 7.72 1.39 13 LC-2-ad 50.25 >30 8.89 36.85 54.26 4.78 14 LNCAP- >100 >30 5.47 75.34 26.66 11.30 clone- FGC 15 MDA- 53.51 >30 3.95 96.51 32.07 4.03 MB-231 16 MDA- 38.49 >30 18.87 98.81 >100 10.71 MB- 435S 17 MFM- 23.97 >30 8.89 37.95 24.10 1.72 223 18 MG63 37.23 >30 7.69 94.52 72.96 1.78 19 NCl- >100 >30 6.89 >100 >100 7.17 H1648 20 NCl- 0.08 0.05 0.04 1.59 0.18 0.02 H1703 21 NCl- 51.93 >30 0.97 83.99 22.54 4.00 H2170 22 NCl-H526 100.00 >30 6.60 >100 >100 7.80 23 NCl-H661 >100 >30 7.09 99.50 86.73 13.34 24 NCl-H716 26.88 >30 1.82 43.75 8.45 4.10 25 SW620 44.47 >30 2.79 71.44 21.92 2.78 26 T.T >100 >30 5.22 >100 >100 4.86 27 TE-15 42.74 >30 7.59 >100 63.57 3.60 28 TE-6 >100 >30 4.29 >100 74.34 4.09 29 Li-7 39.55 >30 5.35 47.24 34.46 5.43 30 JHH1 35.42 >30 8.15 >100 >100 12.16 31 JHH2 >100 >30 28.93 >100 >100 29.54 32 JHH4 95.87 >30 2.62 42.53 39.44 10.61 33 JHH5 48.64 >30 15.21 >100 >100 10.04 34 JHH6 41.72 >30 3.00 67.74 23.58 4.11 35 JHH7 20.65 >30 1.81 45.90 9.98 1.83 36 HUH1 31.86 >30 4.42 66.83 21.21 3.25 37 HUH6 28.23 10.57 0.13 53.79 >100 >30 38 HUH7 23.60 >30 3.22 60.04 22.05 1.67 39 Hep3B2.1-7 36.22 >30 1.59 69.84 13.00 1.96 40 HEPG2 21.44 >30 0.45 19.00 5.83 0.83 41 HLE 36.84 >30 1.98 47.52 36.30 3.93 42 HLF 44.55 >30 3.98 52.55 24.41 3.65 43 Alexander >100 >30 5.50 97.15 98.04 4.09 cells 44 SK-HEP-1 >100 >30 4.59 63.99 29.18 7.11 45 SNU182 >100 19.50 19.88 >100 >100 15.16 46 SNU354 40.58 >30 3.91 78.78 30.18 4.58 47 SNU387 90.22 30.32 12.79 >100 >100 12.32 48 SNU398 50.06 >30 2.61 96.51 18.13 1.91 49 SNU423 36.88 10.89 9.03 >100 >100 9.24 50 SNU449 60.29 >30 5.21 >100 31.63 10.19 51 SNU475 57.46 >30 4.85 81.33 40.68 5.37 52 SNU739 64.93 >30 4.56 73.81 37.40 5.45 53 SNU761 80.13 >30 9.53 85.01 >100 8.98 54 SNU886 60.95 >30 1.47 96.17 22.02 4.48 Note: cell lines 29-54 in Table 1 show the responses of liver cancer cell lines to each compound.
(80) TABLE-US-00004 TABLE 2 Summary of IC50 (μM) values of seven compounds against 54 commercial tumor cell lines Tumor cell line Tumor cell Compound Compound number line name Compound 2 Compound 3 Compound 5 Compound 6 Compound 9 10 11 1 22RV1 7.81 10.32 >100 >100 >100 6.21 90.12 2 AN3CA 0.45 0.64 45.63 55.37 8.42 0.24 20.15 3 CCRF-CEM 6.33 10.25 70.32 90.47 30.19 1.74 40.74 4 DLD1 13.97 20.33 >100 >100 >100 7.53 88.39 5 DU145 4.89 7.38 55.37 63.26 34.51 1.34 26.38 6 HCCLM3 15.75 25.67 >100 >100 >100 8.97 74.26 7 HT1080 19.29 25.79 >100 >100 >100 8.82 94.37 8 HT55 20.45 31.31 >100 >100 >100 14.87 >100 9 HuTu80 5.62 7.33 48.44 61.04 73.46 2.63 23.09 10 K562 7.13 16.94 56.05 74.33 >100 3.04 33.35 11 KASUMI-1 0.12 0.19 3.21 4.94 0.23 0.042 1.21 12 KM12 2.94 4.63 45.69 70.02 21.79 1.47 28.54 13 LC-2-ad 13.46 22.37 51.28 67.81 70.52 6.38 27.49 14 LNCAP-clone- 6.34 15.38 >100 >100 >100 5.13 70.91 FGC 15 MDA-MB-231 5.02 8.51 >100 >100 81.09 3.17 80.36 16 MDA-MB- 30.01 46.36 >100 >100 >100 13.34 87.63 435S 17 MFM-223 10.32 21.85 48.27 72.16 38.29 5.63 26.49 18 MG63 15.83 27.06 >100 >100 >100 4.37 77.05 19 NCl-H1648 8.91 13.64 >100 >100 >100 6.06 >100 20 NCl-H1703 0.063 0.15 3.43 7.16 0.33 0.021 0.89 21 NCl-H2170 3.46 7.89 >100 >100 84.03 1.24 72.75 22 NCl-H526 8.94 11.33 >100 >100 >100 4.39 92.37 23 NCl-H661 11.35 21.08 >100 >100 >100 5.47 >100 24 NCl-H716 2.84 6.09 66.69 92.13 43.06 1.93 82.17 25 SW620 4.67 10.38 >100 >100 66.66 3.46 60.28 26 T.T 8.29 19.83 >100 >100 >100 4.37 >100 27 TE-15 8.45 12.74 >100 >100 88.88 6.31 >100 28 TE-6 7.03 11.28 >100 >100 >100 4.11 >100 29 Li-7 7.26 10.07 59.14 82.03 49.27 4.38 35.05 30 JHH1 11.27 14.94 >100 >100 >100 7.43 >100 31 JHH2 36.02 40.73 >100 >100 >100 25.08 93.47 32 JHH4 5.83 12.06 60.34 79.03 >100 2.31 38.68 33 JHH5 21.34 30.97 >100 >100 >100 12.38 87.65 34 JHH6 4.38 7.51 79.26 >100 65.32 2.46 54.39 35 JHH7 2.84 5.69 57.79 83.16 42.16 1.65 40.03 36 HUH1 6.71 7.93 82.34 >100 52.43 3.47 50.57 37 HUH6 0.82 1.55 72.17 88.66 50.26 0.15 46.37 38 HUH7 4.32 6.69 77.85 92.13 30.34 2.89 42.16 39 Hep3B2.1-7 3.43 5.33 76.49 95.61 52.81 1.23 50.43 40 HEPG2 0.64 1.73 30.79 43.35 10.96 0.37 11.14 41 HLE 2.69 4.72 53.42 68.88 53.27 1.26 55.21 42 HLF 4.94 7.73 63.92 82.11 63.36 3.87 49.35 43 Alexandercells 5.21 12.67 >100 >100 >100 5.31 79.46 44 SK-HEP-1 9.25 15.82 85.08 >100 >100 2.75 55.39 45 SNU182 32.07 54.16 >100 >100 >100 13.28 >100 46 SNU354 7.01 16.75 >100 >100 67.29 4.57 63.26 47 SNU387 19.38 40.15 >100 >100 >100 10.49 >100 48 SNU398 6.37 17.06 >100 >100 82.71 2.99 78.96 49 SNU423 14.38 26.06 >100 >100 >100 8.62 >100 50 SNU449 8.39 14.62 >100 >100 89.95 4.91 >100 51 SNU475 6.37 9.62 >100 >100 77.76 3.88 76.07 52 SNU739 6.33 8.05 >100 >100 >100 3.87 63.96 53 SNU761 15.28 29.06 >100 >100 >100 9.04 76.95 54 SNU886 3.75 9.06 >100 >100 >100 1.45 >100 Note: Cell lines 29-54 in Table 2 shows the response of the liver cancer cell line to each compound
(81) As can be seen from the results of Tables 1 and 2, the IC50 values of compounds 1, 2, 3, and 10 are close to those of Linifanib in almost all tumor cell lines, and the modification of these four compounds hardly functions to block the activity of Linifanib. The modification of the other 8 compounds (compounds 4, 5, 6, 7, 8, 9, 11 and 12) successfully blocked the activity of Linifanib in inhibiting tumor cell proliferation, and the difference in IC50 values between these 8 compounds and the Linifanib is more than 5 times in most tumor cells. There are 3 strains sensitive to Linifanib, KASUMI-1 (leukemia cells), NCI-H1703 (lung cancer cells) and AN3-CA (endometrial cells), with IC50 values of 0.01, 0.02 and 0.19 μM, respectively. The IC50 values for the precursor Linifanib-C12-AA5 were 0.10, 0.08 and 3.14 μM, respectively, with a difference of 10, 4 and 16.7 times, respectively.
(82) Among the 54 commercial tumor cell lines, 26 were liver cancer cell lines, and nearly half of the liver cancer cell lines (12/26, 46%) were moderately sensitive to Linifanib, IC50<5 μM, and at the same time for most of the liver cancer cell lines (15/26, 58%), the IC50 values of precursor Linifanib-C12-AA5 were more than 8 times different than the IC50 values of Linifanib, and almost all liver cancer cells did not respond to the intermediates, as shown Table 1.
Example 45 In Vitro Stability Study (Plasma/Liver Homogenate/Spleen Homogenate)
(83) The purpose of this example was to investigate the stability of the precursor Linifanib-C12-AA5 (compound 8) and the intermediate Linifanib-C12-Asp (compound 12) in plasma, liver homogenate and spleen homogenate (precursor Linifanib-C12-AA5 metabolizes into the intermediates Linifanib-C12-Asp and Linifanib; the intermediate Linifanib-C12-Asp is further metabolized to form Linifanib), and the metabolites were analyzed quantitatively, and the stability of the incubation system was verified with positive drugs, providing references for compound drug evaluation.
(84) 1. Instruments and Materials
(85) Instrument: AP13000 LC/MS, ABI Materials: Male SD rats (200-250 g), Beijing Vital River Test samples: positive drugs and their metabolites M1 and M2, Linifanib-C12-AA5 and its metabolites Linifanib-C12-ASP and Linifanib.
2. Stability study of Linifanib-C12-AA5 and Linifanib-C12-Asp 2.1 Linifanib-C12-AA5 and Linifanib-C12-Asp plasma incubation stability study protocol Test animal Type: SD rat; Quantity: 2 Sex: male; weight: 200-250 g;
(86) Experimental Procedure 1. Animal blood was collected, blood samples were placed in EDTA anticoagulation tubes, centrifuged at 3000 g for 15 min at 4° C., plasma was separated, and 2 blood samples were mixed in equal volumes; 2. Weighed and dissolved a certain amount of Linifanib-C12-AA5/Linifanib-C12-Asp in DMSO:MeOH (2:8), prepared 200 μM mother liquor by purity, and added the compound to the plasma to a final concentration of 2 μg/mL. The organic ratio in the system was not more than 0.5%. 3. Incubated in a 37° C. water bath and set the sampling points to 0, 0.5, 1, 2, 4, 6, and 8 h. At each sampling point, 100 μL of each sample was added to 300 μL of acetonitrile (with internal standard) for precipitation, centrifuged at 12,000 rpm for 5 min, and 200 μL of supernatant was taken for analysis by LC-MS/MS. 4. Configured the standard curve to quantify Linifanib, Linifanib-C12-Asp and residual Linifanib-C12-AA5. 2.2 Linifanib-C12-AA5 and Linifanib-C12-Asp liver/spleen homogenate incubation stability study protocol Test animal Type: SD rat; Quantity: 2 Sex: Male; Weight: 200-250 g
(87) Experimental Procedure 1. Biological analysis method: established the biological analysis method of the compound Linifanib-C12-Asp and Linifanib; 2. Rat liver/spleen was taken on the day of the experiment, two pieces were cut and mixed, and homogenized with 4 volumes (1 g: 4 mL) phosphate buffer (pH 7.4), and the temperature was controlled to not exceed 10° C. in the homogenization process; quantitative detection of protein, and controlled liver and spleen protein concentration. 3. Added the drug Linifanib-C12-AA5, Linifanib-C12-Asp to the liver/spleen homogenate to a concentration of 1000 ng/mL, the incubation volume was 1 mL, and controlled the concentration of the organic solvent in the incubation system to not exceed 0.5%. 4. Incubated in a 37° C. water bath and took 100 μL of samples at 0, 0.5, 1, 2, 4, 6, and 8 h, respectively. After pre-processing the samples with a definite method, the LC-MS/MS analysis was carried out. 5. Prepared a standard curve with blank liver/spleen homogenate to quantify Linifanib-C12-Asp and Linifanib.
3. Stability Study Results
(88) 3.1 Plasma Stability Test Results Positive drugs were metabolized in plasma to their metabolites as expected over time, indicating that the plasma system was stable and the subsequent test results were reliable. The results are shown in Table 3. As can be seen from Table 3, Linifanib-C12-AA5 was stable in plasma and did not produce the intermediates Linifanib-C12-Asp and Linifanib; the intermediate Linifanib-C12-Asp was stably incubated in plasma, and the level of Linifanib produced was close to zero.
(89) TABLE-US-00005 TABLE 3 Experimental results of stability study of precursor and intermediate compounds in rat plasma Intermediate Linifanib produced produced by Residual Linifanib produced by concentration by precursor precursor intermediate intermediate ng/ml Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 0 h 0.961 0 1.96 0 1900 2180 24.2 23.7 0.5 h 0 0 0 0 2100 2290 24.7 24.4 1 h 0 0 0 0 2180 2290 23.6 24.1 2 h 0 0 0 0 2160 2110 22.4 20.7 4 h 0 0 0 0 2210 2270 24.9 24.3 6 h 0 0 0 0 2070 1880 22 21.9 8 h 0 0 0 0 1940 2060 25.1 20.8
(90) 3.2 Liver Homogenate Stability Results The positive drug was metabolized to its metabolite in the liver homogenate as expected over time, and the liver homogenate system was stable, and the subsequent detection results were reliable. The results of liver homogenate stability of the precursors and intermediates are shown in Tables 4-5 and
(91) TABLE-US-00006 TABLE 4 The First Stability Experiment of Liver Homogenate Intermediate Linifanib produced produced by Residual Linifanib produced by concentration by precursor precursor intermediate intermediate ng/ml Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 0 h 56.4 37.7 9.81 5.48 1130 1260 24.6 21.4 0.5 h 124 54.9 28.7 27.8 1000 970 41.6 70.0 1 h 1130 946 61.4 101 2 h 70.0 96.4 88.2 81.8 1000 602 80.1 135 4 h 60.6 47.9 119 119 294 456 123 84.6 6 h 52.0 63.6 197 179 452 226 129 183 8 h 55.9 48.0 201 196 461 185 139 190
(92) TABLE-US-00007 TABLE 5 The Second Stability Experiment of Liver Homogenate Intermediate Linifanib produced produced by Residual Linifanib produced by concentration by precursor precursor intermediate intermediate ng/ml Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 0 h 23.3 14.3 10.2 4.48 649 634 14.2 13.4 0.5 h 15.4 14.8 15.2 15.5 452 469 32.6 29.7 6 h 46.7 43.8 115 113 309 340 112 108
(93) It can be seen from Tables 3-4 and
(94) —3.3 Spleen Homogenization Stability Results
(95) The positive drug was metabolized into its metabolite in the spleen homogenate as expected over time, and the spleen homogenate system was stable, and the subsequent detection results were reliable. The spleen homogenate stability results for the precursors and intermediates are shown in Tables 6-7 and
(96) TABLE-US-00008 TABLE 6 First Stability Experiment of Spleen Homogenate Intermediate Linifanib produced produced by Residual Linifanib produced by concentration by precursor precursor intermediate intermediate ng/ml Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 0 h 53.6 47.9 2.84 2.43 900 1020 36.1 19.4 0.5 h 329 389 18.3 22.8 959 26.7 1 h 582 55.6 2 h 618 79.6 755 681 43.7 51.1 4 h 456 547 63.0 70.3 610 589 50.4 58.5 6 h 398 376 68.2 60.8 470 467 54.0 61.6 8 h 454 572 80.2 99.3 499 506 57.0 64.2
(97) TABLE-US-00009 TABLE 7 Second Stability Experiment of Spleen Homogenate Intermediate Linifanib produced produced by Residual Linifanib produced by concentration by precursor precursor intermediate intermediate ng/ml Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 Repeat 1 Repeat 2 0 h 59.8 66 8.09 6.81 812 844 18.4 18.5 0.5 h 397 445 28.9 29.2 770 744 29.1 27.5 6 h 411 442 108 108 496 483 69.7 67.3
(98) It can be seen from Tables 6-7 and
(99) In addition, the accumulation concentration of the intermediate in the spleen homogenate was higher, and the amount of the produced Linifanib was less than that in the liver homogenate.
(100) From the above results of the in vitro stability study, the following conclusions can be drawn:
(101) (1) The precursor Linifanib-C12-AA5 and the intermediate Linifanib-C12-Asp are stable in plasma.
(102) (2) The metabolic pathway of the precursor Linifanib-C12-AA5 in vitro is basically clear, that is, it is metabolized to the intermediate Linifanib-C12-Asp by PSMA, and then the intermediate Linifanib-C12-Asp is metabolized to the active drug Linifanib by some amide esterase.
(3) The active drug Linifanib is produced more in the liver homogenate, as shown in