PIPETTE WITH ADJUSTABLE PIPETTING VOLUME AND LOCKING SYSTEM
20230271174 · 2023-08-31
Inventors
- Julian Bikmohammadi (Hamburg, DE)
- Andreas Ratzka (Hamburg, DE)
- Elena Schmidt (Geesthacht, DE)
- Florian Tesch (Hamburg, DE)
- Christian Baasch (Großhansdorf, DE)
- Christiane Markau (Hamburg, DE)
- Michael Strutz (Neuendorf, DE)
- Anne Oehlmann (Hamburg, DE)
- Kevin Höricke (Hamburg, DE)
- Ulrike Gast (Ammersbek, DE)
- Olaf Sauer (Bad Gottleuba-Berggießhübel, DE)
Cpc classification
B01L3/0275
PERFORMING OPERATIONS; TRANSPORTING
B01L2200/14
PERFORMING OPERATIONS; TRANSPORTING
B01L3/0224
PERFORMING OPERATIONS; TRANSPORTING
International classification
Abstract
The invention relates to a pipette with a body for receiving a displacement device, the displacement device comprising a displacement chamber and a movable boundary element for bounding a pipetting stroke of the pipette. The pipette furthermore has an operating element for setting the pipetting volume of the pipette by movement of the boundary element in the body and for performing a pipetting operation, the operating element being in operative connection with a piston movable in the displacement chamber of the displacement device. The pipette furthermore comprises a counter for displaying the set pipetting volume and a locking mechanism for locking and/or unlocking volume setting by the operating element.
It is provided that, on actuation of the operating element, the operating element is configured to perform a pipetting operation, to transfer the locking mechanism into a locked position and to lock adjustment of the pipetting volume.
Claims
1. A pipette (10) comprising a body (20) for receiving a displacement device (22), the displacement device (22) comprising a displacement chamber (24) and a movable boundary element (26) for bounding a pipetting stroke of the pipette (10), an operating element (12) for setting the pipetting volume of the pipette (10) by movement of the boundary element (26) in the body (20) and for performing a pipetting operation, the operating element (12) being in operative connection with a piston (28) movable in the displacement chamber (24) of the displacement device (22), a counter (30) for displaying the set pipetting volume, a locking mechanism (40) for locking and/or unlocking volume setting by the operating element (12), characterized in that, on actuation of the operating element (12), the operating element (12) is configured to perform a pipetting operation, to transfer the locking mechanism (40) into a locked position and to lock adjustment of the pipetting volume.
2. The pipette (10) according to claim 1, characterized in that the locking mechanism (40) comprises a locking element (41) for unlocking volume setting.
3. The pipette (10) according to claim 1, characterized in that latching means (44) are provided which hold the locking mechanism (40) in an unlocked switching position after an unlocking operation.
4. The pipette (10) according to claim 3, characterized in that the operating element (12) comprises an operating button (14) or is in operative connection with a operating button (14) which releases the latching means (44) on performance of a pipetting operation and transfers the locking mechanism (40) into the locked position.
5. The pipette (10) according to claim 1, characterized in that the locking mechanism (40) comprises a locking element (41) and a connecting element (46) which connects the locking element (41) with a coupling element (32) which connects the counter (30) with a setting mechanism (34) for moving the boundary element (26) in the displacement chamber (24).
6. The pipette (10) according to claim 5, characterized in that, in an unlocked switching position of the locking mechanism (40), the coupling element (32) transmits a rotational movement of the operating element (12) for adjusting the pipetting volume to the counter (30).
7. The pipette (10) according to claim 5, characterized in that the locking mechanism (40) engages form-fittingly in the coupling element (32) and, via the form-fitting connection, locks rotation of the operating element (12) and any associated displacement of the boundary element (26) of the displacement chamber (24).
8. The pipette (10) according to claim 7, characterized in that the form-fitting connection engages by gear teeth (48) on the locking mechanism (40) in gear teeth (36) on the coupling element (32).
9. The pipette (10) according to claim 1, characterized in that the locking mechanism (40) comprises a locking element (41), the locking element (41) or a component connected to the locking element (41) being displaced in the axial direction along a longitudinal axis of the pipette (10) in an unlocking direction during a locking operation.
10. The pipette (10) according to claim 9, characterized in that the locking element (41) or a component connected to the locking element (41) is axially displaced on performance of a pipetting operation in a direction opposed to the unlocking direction and thereby locks volume adjustment of the pipette (10).
11. The pipette (10) according to claim 1, characterized in that the pipette (10) comprises a rotary element (50) and/or a sliding element (58) and the locking mechanism (40) comprises a locking element (41), the rotary element (50) and/or the sliding element (58) being in operative connection with the locking element (41) in such a manner that the rotary element (50) and/or the sliding element (58) rotates into a travel path of the operating element (12) when the locking element (41) is brought into an unlocked position.
12. The pipette (10) according to claim 11, characterized in that the rotary element (50) and the locking element (41) are connected to one another via a slotted link mechanism (52).
13. The pipette (10) according to claim 11, characterized in that the rotary element (50) is fastened to the body (20) of the pipette (10) by way of a shield (54).
14. The pipette (10) according to claim 2, characterized in that the locking element (41) is arranged in a recess (60) of the body (20).
15. The pipette (10) according to claim 1, characterized in that a finger hook (62) is arranged on the body (20), the locking mechanism (40) being arranged on a side of the body (20) opposite the finger hook (62).
16. The pipette (10) according to claim 1, characterized in that the locking mechanism (40) comprises a return spring (56) which is tensioned during an unlocking operation of the locking mechanism (40).
17. The pipette (10) according to claim 1, characterized in that the locking element (41) has a frame (74) and an elastic tongue (68) formed on the frame (74), wherein, on the elastic tongue (68), a hook (70) is provided which projects radially inward beyond the elastic tongue (68).
18. The pipette (10) according to claim 17, characterized in that a latching tab (64) is provided on the locking element (41), which latching tab is configured to engage in a recess (66) on the body (20) of the pipette (10) or in a recess (66) on a shield (54) inserted into the body (20), such that the locking element (41) is held in an unlocked position of the locking mechanism (40) in which volume setting of the pipette (10) is possible.
19. The pipette (10) according to claim 17, characterized in that a locking slide (42) is arranged on the pipette (10), the locking slide (42) bringing about on actuation an axial displacement of the locking element (41) along a longitudinal axis of the pipette (10), such that a form-fitting connection between the locking element (41) and a counterpart is released and locking of the volume setting of the pipette (10) is released.
Description
[0055] The invention is explained below in exemplary embodiments with reference to the accompanying drawings. Identical components or components with the same function are denoted with the same reference numerals in the various figures. In the drawings:
[0056]
[0057]
[0058]
[0059]
[0060]
[0061]
[0062]
[0063]
[0064]
[0065] The operating element 12 firstly serves to set a pipetting volume as the volume of liquid which, during a pipetting operation, is to be drawn up by the pipette 10 and then delivered back out. The pipetting volume is set by rotating the operating element 12, the set pipetting volume being increased by rotation of the operating element 12 in a first direction of rotation and decreased by rotation in a direction of rotation opposed to the first direction of rotation. Depending on the design, the settable pipetting volume is between a minimum pipetting volume and a maximum pipetting volume. The operating element 12 secondly serves to draw up and subsequently deliver liquid during a pipetting operation, an operating button 14 of the operating element 12 being displaced in the axial direction along a center axis of the pipette. The operating element 12 thirdly serves to eject the used pipette tip from a receptacle on the pipette lower part 17 after a pipetting operation. All three functions are preferably combined in the operating button 14 such that operation can proceed simply and conveniently with just a single operating button 14. Alternatively, the function can also be divided among a number of components of the operating element 12 and in particular an additional button for ejecting a used pipette tip can be provided next to the operating button 14. It is furthermore possible for the operating element 12 to have an operating button 14 for drawing up and delivering the liquid and an element displaced by the operating button 14 for setting the pipetting volume.
[0066] The pipette 10 comprises a body 20 for receiving a displacement device 22, the displacement device 22 comprising a displacement chamber 24 and a movable boundary element 26 for bounding the pipetting stroke. The operating element 12 is configured for setting a pipetting volume of the pipette 10 by movement of the boundary element 26 in the body 20 and for performing a pipetting operation. The operating element 12 is in operative connection via a lifting rod 21 with a piston 28 movable in the displacement chamber 24 of the displacement device 22. The pipette 10 furthermore comprises a counter 30 for displaying the set pipetting volume and a locking mechanism 40 for locking and/or unlocking volume setting by the operating element 12. It is provided that, on actuation of the operating element 12, the operating element 12 is configured to perform a pipetting operation, to transfer the locking mechanism 40 into a locked position and to lock adjustment of the pipetting volume. A restoring spring 18 for the operating element 12 is furthermore provided in order to return the operating element 12 to the initial position once it has been relieved of load.
[0067] The locking mechanism 40 comprises a locking element 41 and a locking slide 42 for unlocking the locking mechanism. The locking mechanism 40 furthermore comprises a latching element 44 with which the locking slide and/or the locking element 41 can be temporarily fixed in an unlocked switch position in order to enable simple setting of pipetting volume by rotation of the operating element 12.
[0068] A transparent display window 31 is set into the body 20 to provide a view of the counter 30 from the outside and allow the set pipetting volume to be read. A movable boundary element 26 for the lifting rod 21 is provided in the body 20, a shoulder on the lifting rod 21 resting against the movable boundary element 26 and thereby limiting the stroke of the piston 28. Pipetting volume is set by moving the boundary element 26.
[0069] The pipette lower part 17 comprises a displacement chamber 24, into which the piston 28 actuatable via the operating element 12 and connected to the lifting rod 21 extends. The pipette lower part 17 has a clamping zone 19 for receiving a pipette tip, wherein it is possible to detach the pipette tip from the clamping region 19 and thereby eject it by actuation of the operating element 12 via the ejector rod 37.
[0070] In order to change the pipetting volume of the pipette 10, the locking slide 42 of the locking mechanism 40 is first brought into an unlocked position. An unlocked position should be taken to mean a position of the locking mechanism in which rotation of the operating element 12 and an associated change of the pipetting volume are possible. Once the desired pipetting volume has been set, the operating element 12 is then actuated to perform a pipetting operation. Actuation of the operating element 12 should in this connection be taken to mean pressing the operating element 12 with an associated axial displacement of the operating element 12 along a longitudinal axis of the pipette 10. The longitudinal axis extends in the plane of the drawing from the top downward and leads from the operating element 12 through the pipette upper part 16 and the pipette lower part 17 down to a receiving opening at the end of the pipette lower part 17 remote from the upper part 16. On actuation, the path of the operating element 12 is divided in three actuation zones.
[0071] A first actuation zone serves for drawing up and delivering a liquid with the pipette and is also denoted stroke H. Starting from an unloaded starting position, the operating element 12 is pressed in the axial direction along a travel path into the body 20 of the pipette 10 by for example 2 mm to 16 mm depending on the set pipetting volume.
[0072] The first actuation zone is followed by a second actuation zone, also denoted overstroke, which in particular serves to blow out from the pipette 10 any residual liquid possibly still adhering to a pipette tip mounted on a receptacle on the body 20 of the pipette 10 after liquid delivery. This “overstroke” proceeds by an additional movement of the operating element 12 in the axial direction beyond the endpoint of the stroke H on drawing up liquid by for example a further 4 to 6 mm. In the overstroke zone, a tripping element of the locking mechanism 40 protrudes into the travel path of the operating element 12 such that the locking mechanism 40 is tripped and a form-fitting connection between the locking element 41 and the coupling element 32 is produced when the overstroke is activated by the operating element 12. Volume adjustment is thus locked from this point in time onward in the pipetting operation. Such a locking operation can either be performed before the liquid is drawn up in order to fix the set pipetting volume, or can be initiated during the pipetting operation, in particular when blowing liquid out after liquid delivery.
[0073] If the operating element 12 is pressed further in the axial direction into the body 20, an ejector mechanism connected to the operating element 12 pushes the used pipette tip from a receptacle on the body 20 in a third actuation zone in order to eject the used pipette tip. It is provided to this end that a tripping element is formed at an end of an ejector rod 37 of the ejector mechanism facing the operating element 12, which tripping element projects into the travel path of the operating element 12 and initiates ejection of the pipette tip when the operating element 12 is pressed beyond the limit stop of the overstroke further into the body 20 of the pipette 10.
[0074] The actuation zones are preferably delimited from one another by a limit stop and an associated change in operating force in order to provide the user with sensitive feedback regarding the action currently being performed.
[0075]
[0076] Alternatively to a rotary element 50 or in addition to a rotary element 50, a sliding element 58 may also be provided which is introduced into the displacement path of the operating element 12 and, on actuation of the operating element 12 in the context of a pipetting operation, i.e. by axial displacement of the operating element 12 by pressure on the operating button 14, is activated and pushes the locking element 41 in the axial direction into the coupling element 32 in order to form a form-fitting lock. A shoulder of the ejector rod 37 arranged at one end of the ejector rod 37 is furthermore located in the displacement path of the operating element 12 such that, once the operating element 12 has been displaced sufficiently far along the longitudinal axis of the pipette 10, a pipette tip received on the clamping zone 19 is ejected in known manner.
[0077]
[0078]
[0079] A finger hook 62 is formed on the body 20 so that the user can hold the pipette 10 in one hand and operate it with this hand. When a hand is wrapped around the body 20, the finger hook 62 accordingly rests on the user's index or middle finger such the user can preferably use their thumb to selectively actuate the locking slide 42 or the operating button 14 in order either to set the pipetting volume or to perform a pipetting operation.
[0080]
[0081] As is apparent in
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LIST OF REFERENCE SIGNS
[0085] 10 Pipette
[0086] 12 Operating element
[0087] 14 Operating button
[0088] 16 Upper part
[0089] 17 Lower part
[0090] 18 Restoring spring
[0091] 19 Clamping zone for pipette tip
[0092] 20 Body
[0093] 21 Lifting rod
[0094] 22 Displacement device
[0095] 24 Displacement chamber
[0096] 26 Movable boundary element
[0097] 28 Piston
[0098] 30 Counter
[0099] 31 Display window
[0100] 32 Coupling element
[0101] 33 Axle securing means
[0102] 34 Setting mechanism
[0103] 35 Volume setting sleeve
[0104] 36 Gear teeth on coupling element
[0105] 37 Ejector rod
[0106] 38 Gearwheel
[0107] 40 Locking mechanism
[0108] 41 Locking element
[0109] 42 Locking slide
[0110] 44 Latching element
[0111] 46 Connecting element
[0112] 48 Gear teeth (on locking mechanism)
[0113] 50 Rotary element
[0114] 51 Pivot point
[0115] 52 Slotted link mechanism
[0116] 54 Shield
[0117] 56 Return spring
[0118] 58 Sliding element
[0119] 60 Recess
[0120] 62 Finger hook
[0121] 64 Latching tab
[0122] 66 Recess
[0123] 68 Tongue
[0124] 70 Hook
[0125] 72 Surface on the shield
[0126] 74 Frame
[0127] H Stroke by axial movement/pressure on the operating element
[0128] V Volume adjustment by rotational movement of the operating element