VETERINARY COMPOSITIONS FOR THE TREATMENT AND/OR PREVENTION OF PROTOZOAN DISEASES AND METHODS OF PREPARATION THEREOF

Abstract

Veterinary compositions comprising an essential oil such as cedar oil, tea oil, cardamom oil, lavender oil, cubeb oil, eucalyptus oil, mint oil, juniper oil, cypress oil, lemongrass oil, spruce oil, pine oil, fir oil, Douglas fir oil, wormwood oil, caraway oil, cumin oil, patchouli oil, sage oil, Inula oil, tansy oil, angelica oil calamus oil, ginger oil, thyme oil, Origanum oil, rosemary oil, nutmeg oil, coriander oil, geranium oil, carrot seed oil, yarrow herb or flower oil are disclosed. The essential oil may be present in the form of a complex with an organic acid or a mixture of organic acids including valerian, isovalerian, lactic, butyric, acetic, propionic, formic, benzoic, pelargonic, salicylic, malonic, citric, phthalic, tartaric, oxalic, malic, shikintic, fumaric, almond, cinnamic or derivatives thereof, and a metal including molybdenum, cobalt, nickel, chromium, zinc, bismuth, copper, manganese, selenium, iron, or salts or oxides thereof. The compositions may be for the treatment and/or prevention of protozoan diseases in animals. Methods of manufacturing the veterinary compositions are also disclosed.

Claims

1.-8. (canceled)

9. A veterinary composition to treat and/or prevent protozoan diseases in animals, the composition comprising: an essential oil including cedar oil, tea oil, cardamom oil, lavender oil, cubeb oil, eucalyptus oil, mint oil, juniper oil, cypress oil, lemongrass oil, spruce oil, pine oil, fir oil, Douglas fir oil, wormwood oil, caraway oil, cumin oil, patchouli oil, sage oil, Inula oil, tansy oil, angelica oil calamus oil, ginger oil, thyme oil, Origanum oil, rosemary oil, nutmeg oil, coriander oil, geranium oil, carrot seed oil, yarrow herb, or flower oil, the essential oil present in a form of a complex with an organic acid or a mixture of organic acids, and a metal, the organic acid or mixture of organic acids including valerian, isovalerian, lactic, butyric, acetic, propionic, formic, benzoic, pelargonic, salicylic, malonic, citric, phthalic, tartaric, oxalic, malic, shikimic, fumaric, almond, cinnamic, or derivatives thereof, and a metal including molybdenum, cobalt, nickel, chromium, zinc, bismuth, copper, manganese, selenium, iron, or salts or oxides thereof.

10. The composition of claim 9, wherein the complex is with the mixture of organic acids and the mixture includes acetic acid, propionic acid, lactic acid and formic acid.

11. The composition of claim 10, wherein acids in the mixture of organic acids are mixed at a ratio of 1:1:1:1:1.

12. The composition of claim 10, wherein the essential oil includes eucalyptus oil, cedar oil, yarrow oil, wormwood oil, Origanum oil, or lavender oil.

13. The composition of claim 9, wherein the essential oil includes eucalyptus oil.

14. The composition of claim 9, wherein the essential oil includes cedar oil.

15. The composition of claim 9, wherein the essential oil includes yarrow oil.

16. The composition of claim 9, wherein the essential oil includes wormwood oil.

17. The composition of claim 9, wherein the essential oil includes Origanum oil.

18. The composition of claim 9, wherein the essential oil includes lavender oil.

19. A method of manufacturing a veterinary composition to treat and/or prevent protozoan diseases in animals, the method comprising: a) mixing at least one essential oil, and an organic acid or a mixture of organic acids at a ratio of 80:1 to 1:80 by weight, the essential oil including cedar oil, tea oil, cardamom oil, lavender oil, cubeb oil, eucalyptus oil, mint oil, juniper oil, cypress oil, lemongrass oil, spruce oil, pine oil, fir oil, Douglas fir oil, woanwood oil, caraway oil, cumin oil, patchouli oil, sage oil, Inula oil, tansy oil, angelica oil calamus oil, ginger oil, thyme oil, Origanum oil, rosemary oil, nutmeg oil, coriander oil, geranium oil, carrot seed oil, yarrow herb, or flower oil and the organic acid or the mixture of organic acids including valerian, isovalerian, lactic, butyric, acetic, propionic, formic, benzoic, pelargonic, salicylic, malonic, citric, phthalic, tartaric, oxalic, malic, shilcimic, fumaric, almond, cinnamic, or derivatives thereof; b) adding a catalyst and a metal including molybdenum, cobalt, nickel, chromium, zinc, bismuth, copper, manganese, selenium, iron, or salts or oxides thereof to form a mixture; c) heating the mixture to form a reaction product; d) allowing the reaction product to cool; and e) filtering the reaction product.

20. The method of claim 19, wherein the at least one essential oil is mixed with the organic acid or the mixture of organic acids at a ratio of 1:1 by weight.

21. The method of claim 19, wherein the at least one essential oil is mixed with the mixture of organic acids and the mixture of organic acids includes acetic acid, propionic acid, lactic acid and formic acid.

22. The method of claim 21, wherein acids in the mixture of organic acids are mixed at a ratio of 1:1:1:1.

23. The method of claim 19, wherein the catalyst includes cobalt sulphate, ammonium molybdate and manganese chloride or sulphate.

24. The method of claim 19, wherein the mixture is heated under reflux.

25. The method of claim 19, wherein the mixture is heated for 20 to 120 minutes.

26. The method of claim 19, wherein the reaction product is cooled for 10 to 24 hours.

27. The method of claim 19, wherein the mixture is heated to a boiling point of the mixture.

28. The method of claim 19, wherein the essential oil includes eucalyptus oil, cedar oil, yarrow oil, wormwood oil, Origanum oil, or lavender oil.

Description

DETAILED DESCRIPTION

[0074] The compositions and methods are set out in detail in the following examples, wherein all tests and experimental procedures described below were carried out using commercially available test kits, reagents and devices, following the recommendations of the manufacturers of the kits, reagents and devices used, unless otherwise expressly indicated. All test parameters were measured using standard, well-known methods used in the field.

[0075] All raw materials used in the study are approved for both animal and human nutrition by the relevant directives and authorities. The selection of raw materials was made on the basis of Codex Alimentarius, i.e. the Codex Alimentarius established by FAO and WHO, Der Deutsche Arzneimittel-Codex (DAC), guidelines of the European Food Safety Authority (EFSA) and Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Aug. 2003 on additives for use in animal nutrition. In addition, the essential oils used in the study met the requirements of the European Pharmacopoeia, the Swiss Pharmacopoeia and Der Deutsche Arzneimittel-Codex (DAC).

[0076] For in vitro tests of antiprotozoal activity of the composition five reference organisms representing taxonomic groups to which pathogenic protozoa belong were selected, i.e: [0077] Amoeba proteus—Chaos diffluens—a protozoan of the order Euamoebida, belonging to fifth supergroup of the Amoebozoa, living in waters. [0078] Paramecium caudatum—a slipper animalcule representing the Ciliata orachs, living in waters. [0079] Gregarina blattarum—gregarine isolated from cockroaches, representing the phylum Apicomplexa, living in the digestive tracts or body cavities of invertebrates. [0080] Euglena gracilis—a protozoan living in water, representing the flagellates—Mastigophora, family Euglenaceae. [0081] Trichomonas hominis—a protozoan living in the human colon, representing the Trichomonadidae.

[0082] Amoeba, Paramecium, Trichomonas and Euglena were observed under a microscope on watch glasses with viscose wool fibers (to facilitate observation) in a drop of water from the culture they came from. Different concentrations of the test compositions were introduced to the test samples, establishing an LD.sub.50 dose (50% mortality) and an LD.sub.100 dose (100% mortality). In all cases, 4-fold replicates of the test were used together with a blank test.

[0083] The gregarines were isolated from the cockroaches and, after being placed on a watch slide, were treated with the products at different concentrations in Ringer's solution. Each sample contained ten individuals. The lethal concentration of the substance for 50% and 100% of the individuals (LD.sub.50, LD.sub.100) within 3 minutes was determined. Isolation of gregarines from cockroaches was performed on the basis of the method of isolation of gregarines from beetles proposed by J. Moraczewski (Moraczewski J.: Exercises in the zoology of invertebrates. 1st Edition, PWN, Warsaw 1974, p. 29-31, p. 285-292).

[0084] Identification of individual protozoa was made on the basis of their descriptions and drawings after W. A. Dogiel and J. Hempel-Zawitkowska (Dogiel W. A.: Invertebrate zoology. 3rd edition, National Agricultural and Forest Publishing House 1972; Hempel-Zawitkowska J., Galka B., Kalińska B., Kamionek M., Komosińska H., Pezowicz E. Podsiadło E., Sulgostowska T.: Zoology for agricultural universities. Scientific Publishing House PWN 2008).

[0085] The compositions, negative controls, and positive controls were dissolved in an aqueous solution of polysorbate 80 (0.05%) before application to a watch slide. No lethal effect of polysorbate 80 at the above concentration was observed.

EXAMPLE 1

A Combination of an Eucalyptus Oil (Eucalyptus globulus Labill.) with an Acid a Ratio of 1:1 and with Copper or Zinc

[0086] In this non-limiting example, the following two compositions were prepared: [0087] a) composition I—i.e., a composition of eucalyptus oil with an acetic acid and copper [0088] b) composition II—i.e., a composition of eucalyptus oil with an acetic acid and zinc

[0089] In order to prepare composition I, 100 ml of acetic acid, 0.6 g of catalyst and 5 g of copper carbonate were added to 100 ml of eucalyptus oil. A mixture of cobalt sulfate, ammonium molybdate and manganese chloride mixed in a ratio of 1:1:1 was used as a catalyst in this example. The mixture was heated at the boiling point, until the color changed, under reflux for 120 minutes. The mixture was then left to cool at a room temperature for 24 hours to obtain a clear solution. The solution may be a one, two or three-phase solution. After this time, the reaction product was filtered through filter paper. Composition II was prepared analogously to composition I, except that 5 g of zinc carbonate was used as the metal. Compositions I and II were then analysed for their antiprotozoal properties. For this purpose, both compositions were diluted: 0.001% to 1%, after which the following protozoa were placed in each dilution: [0090] Amoeba proteus—Chaos diffluens—a protozoan of the order Euamoebida, belonging to fifth supergroup of the Amoebozoa, living in waters. [0091] Paramecium caudatum—a slipper animalcule representing the Ciliata orachs, living in waters. [0092] Gregarina blattarum—gregarine isolated from cockroaches, representing the phylum Apicomplexa, living in the digestive tracts or body cavities of invertebrates. [0093] Euglena gracilis—a protozoan living in water, representing the flagellates—Mastigophora, family Euglenaceae. [0094] Trichomonas hominis—a protozoan living in the human colon, representing the Trichomonadidae.

[0095] Individual acid, catalyst solution, copper carbonate solution, zinc carbonate solution and eucalyptus oil (Eucalyptus globulus Labill.) were additionally analysed for protozoal properties. The control antiprotozoal substances used were CH—chloramphenicol and M—metronidazole. The tested preparations were dissolved in an aqueous solution of polysorbate 80 (0.05%) before being applied to a watch glass. No killing effect of polysorbate 80 in the above mentioned concentration was observed. Observation under a fluorescence microscope with phase contrast was carried out. Protozoicidal activity was considered effective when the death of 50% and 100% of individuals occurred within 3 minutes. The results obtained from the protozoal activity test are presented in Table 1. The results of the analysis showed that the killing and static activity in the compositions and after the reaction was higher than that of the individual substances in the reaction mixtures and complexes. Compositions I and II show many times stronger (potentiation) protozoal activity than each of these components separately. All the ingredients used in the compositions according to the invention are approved for both animal and human nutrition by the relevant directives and authorities, which, combined with their high efficacy, allows their use in the treatment and/or prevention of parasitoses in animals, caused by protozoa, in particular histomonadiasis (caused by Histomonas meleagridis), coccidiosis (caused by Eimeria), cryptosporidiosis (caused by Cryptosporidium), trichomonadiasis (caused by Trichomonas), babesiosis (caused by Babesia), or amoebiasis (caused by Amoeba).

TABLE-US-00001 TABLE 1 LD.sub.50, LD.sub.100 values for compositions I and II, determined for selected protozoa. Sample Catalyst Copper carbonate Zinc carbonate Eucalyptus oil Composition Composition Protozoa CH* M** Acid solution solution solution (Eucalyptus globulus Labill.) I II Euglena LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: gracilis 0.05% LD.sub.100: 0.5% 0.05% 0.15% 0.1% 0.1% 0.004% 0.002% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1.1% 0.1% 0.25% 0.3% 0.2% 0.008% 0.004% Gregarina LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: blattarum LD.sub.100: 0.13% 0.9% 0.07% 0.12% 0.2% 0.08% 0.004% 0.003% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.3% 1.1% 0.3% 0.37% 0.4% 0.1% 0.005% 0.006% Amoeba LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: proteus 0.07% 0.3% 0.6% 0.05% 0.09% 0.15% 0.05% 0.003% 0.005% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 0.5% 1% 1% 0.17% 0.25% 0.07% 0.006% 0.006% Paramecium LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: caudatum 0.001% LD.sub.100: 0.8% 0.8% 0.35% 0.3 0.1% 0.002% 0.006% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.006% 1.25% 1.25% 0.5% 0.5% 0.3% 0.006% 0.008% Trichomonas LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: hominis LD.sub.100: 0.05% 0.8% 0.9% 0.1% 0.25% 0.09% 0.045% 0.025% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1% 1.1% 0.3% 0.4% 0.1% 0.075% 0.035% *CH—chloramphenicol; **M—metronidazole

EXAMPLE 2

A Combination of an Eucalyptus Oil (Eucalyptus globulus Labill.) with an Acid a Ratio of 1:1 and with Manganese

[0096] In order to prepare composition III, 100 ml of propionic acid, 0.6 g of catalyst and 5 g of manganese chloride were added to 100 ml of eucalyptus oil. While, in this non-limiting example, the propionic acid has been used as organic acid component to prepare the composition, other organic acid (e.g. valeric, isovaleric, butyric, formic, benzoic, pelargonic, salicylic, malonic, citric, phthalic, tartaric, oxalic, malic, shikimic, fumaric, almond or cinnamic acid) may be used for preparation of the composition.

[0097] In this example, the catalyst used was a mixture of chromium (III) nitrate, nickel (II) sulfate and selenium sulfide mixed in a ratio of 0.1:100:0.1. The mixture was heated at boiling point, until the colour changed, under reflux for 60 minutes. The resulting mixture was allowed to cool at room temperature for 15 hours to obtain a clear solution, which was then filtered through filter paper.

EXAMPLE 3

A Combination of an Eucalyptus Oil (Eucalyptus globulus Labill.) with an Acid a Ratio of 80:1 and with Copper or Zinc

[0098] In this non-limiting example, the following two compositions were prepared: [0099] a) composition IV—i.e., a composition of eucalyptus oil with a lactic acid and copper [0100] b) composition V—i.e., a composition of eucalyptus oil with a lactic acid and zinc

[0101] Wherein, in order to prepare composition IV, 1 ml of lactic acid, 0.1 g of catalyst (i.e. a mixture of cobalt sulfate, ammonium molybdate and manganese sulfate mixed in ratio of 1:1:1) and 5 g of copper carbonate were added to 80 ml of eucalyptus oil. The mixture was heated at boiling point, until the colour changed, under reflux for 20 minutes. The mixture was then left to cool for 10 hours to obtain a clear solution (one, two or three phase solution). After this time, the reaction product was filtered through filter paper. Composition V was prepared analogously to composition IV, except that 1 g of zinc carbonate was used as the metal. Compositions IV and V were then analysed for their antiprotozoal properties analogously to Example 1. Results are presented in table 2.

TABLE-US-00002 TABLE 2 LD.sub.50, LD.sub.100 values for compositions IV and V, determined for selected protozoa. Sample Catalyst Copper carbonate Zinc carbonate Eucalyptus oil Composition Composition Protozoa CH* M** Acid solution solution solution (Eucalyptus globulus Labill.) IV V Euglena LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: gracilis 0.05% LD.sub.100: 0.5% 0.05% 0.15% 0.1% 0.1% 0.04% 0.035% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1.1% 0.1% 0.25% 0.3% 0.2% 0.07% 0.08% Gregarina LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: blattarum LD.sub.100: 0.13% 0.9% 0.07% 0.12% 0.2% 0.08% 0.05% 0.048% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.3% 1.1% 0.3% 0.37% 0.4% 0.1% 0.1% 0.1% Amoeba LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: proteus 0.07% 0.3% 0.6% 0.05% 0.09% 0.15% 0.05% 0.03% 0.03% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 0.5% 1% 1% 0.17% 0.25% 0.07% 0.05% 0.06% Paramecium LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: caudatum 0.001% LD.sub.100: 0.8% 0.8% 0.35% 0.3 0.1% 0.07% 0.08% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.006% 1.25% 1.25% 0.5% 0.5% 0.3% 0.1% 0.12% Trichomonas LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: hominis LD.sub.100: 0.05% 0.8% 0.9% 0.1% 0.25% 0.09% 0.05% 0.065% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1% 1.1% 0.3% 0.4% 0.1% 0.08% 0.08% *CH—chloramphenicol; **M—metronidazole

EXAMPLE 4

A Combination of a Cedar Oil (Cedrus atlantica (Endl.) Manetti ex Carrière, Cedrus deodara (Roxb. ex D. Don) G. Don, Cedrus libani A. Rich.) with Acid in a Ratio of 1:1 by Weight and with Copper or Zinc

[0102] In this non-limiting example, the following two compositions were prepared: [0103] a) composition VI—i.e., a composition of cedar oil with a propionic acid and copper [0104] b) composition VII—i.e., a composition of cedar oil with a propionic acid and zinc

[0105] In this non-limiting example, propionic acid is used, but other organic acids (e.g., acetic acid, lactic acid, etc.) may also be used. In order to prepare composition VI, 100 ml propionic acid, 0.6 g catalyst (which is cobalt sulfate, ammonium molybdate and manganese chloride mixed in a ratio of 1:10:20) and 5 g of copper carbonate were added to 100 ml of cedar oil. The mixture was heated at the boiling point, until the color changed, under reflux for 100 minutes. The mixture was then left to cool (for 20 hours) to obtain a clear solution (one, two or three phase solution). After this time, the reaction product was filtered through filter paper. Composition VII was prepared analogously to composition VI, except that zinc carbonate was used as the metal. Compositions VI and VII were then analysed for their antiprotozoal properties analogously to Example 1. Results were presented in Table 3. The results of the analysis showed that the killing and static activity in the compositions and after the reaction was higher than that of the individual substances in the reaction mixtures and complexes. Compositions VI and VII show many times stronger (potentiation) protozoal activity than each of these components separately. All the ingredients used in the compositions according to the invention are approved for both animal and human nutrition by the relevant directives and authorities, which, combined with their high efficacy, allows their use in the treatment and/or prevention of parasitoses in animals, caused by protozoa, in particular histomonadiasis (caused by Histomonas meleagridis), coccidiosis (caused by Eimeria), cryptosporidiosis (caused by Cryptosporidium), trichomonadiasis (caused by Trichomonas), babesiosis (caused by Babesia), or amoebiasis (caused by Amoeba).

TABLE-US-00003 TABLE 3 LD.sub.50, LD.sub.100 values for compositions VI and VII, determined for selected protozoa. Sample Catalyst Copper carbonate Zinc carbonate Composition Composition Protozoa CH* M** Acid solution solution solution Cedar oil VI VII Euglena LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: gracilis 0.05% LD.sub.100: 0.5% 0.05% 0.15% 0.1% 0.3% 0.004% 0.003% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1.1% 0.1% 0.25% 0.3% 0.4% 0.008% 0.006% Gregarina LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: blattarum LD.sub.100: 0.13% 0.9% 0.07% 0.12% 0.2% 0.1% 0.003% 0.004% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.3% 1.1% 0.3% 0.37% 0.4% 0.2% 0.006% 0.005% Amoeba LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: proteus 0.07% 0.3% 0.6% 0.05% 0.09% 0.15% 0.08% 0.004% 0.002% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 0.5% 1% 1% 0.17% 0.25% 0.1% 0.005% 0.005% Paramecium LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: caudatum 0.001% LD.sub.100: 0.8% 0.8% 0.35% 0.3 0.2% 0.003% 0.002% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.006% 1.25% 1.25% 0.5% 0.5% 0.3% 0.008% 0.004% Trichomonas LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: hominis LD.sub.100: 0.08% 0.8% 0.9% 0.9% 0.25% 0.25% 0.09% 0.025% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 1% 1.1% 1.1% 0.4% 0.4% 0.15% 0.07% *CH—chloramphenicol; **M—metronidazole

EXAMPLE 5

A Combination of a Cedar Oil (Cedrus atlantica (Endl.) Manetti ex Carrière, Cedrus deodara (Roxb. ex D. Don) G. Don, Cedrus libani A. Rich.) with Acid in a Ratio of 1:80 by Weight and with Copper or Zinc

[0106] In this example, the following two compositions were prepared: [0107] a) composition VIII—i.e., a composition of cedar oil with acetic acid and copper [0108] b) composition IX—i.e., a composition of cedar oil with acetic acid and zinc

[0109] Although in this non-limiting example, copper or zinc were used in the composition, other metals, i.e. molybdenum, cobalt, nickel, chromium, zinc, bismuth, copper, manganese, selenium, iron, their salts, or oxides, can be used as the metal component for preparation the composition.

[0110] In order to prepare composition VIII, 80 ml of acetic acid, 1 g of catalyst (which is cobalt sulfate, ammonium molybdate and manganese sulfate mixed in ratio of 1:90:9) and 5 g of copper carbonate were added to 1 ml of cedar oil.

[0111] The mixture was heated at the boiling point, until the color changed, under reflux for 30 minutes. The mixture was then left to cool (for 10 hours) to obtain a clear solution (one, two or three phase solution). After this time, the reaction product was filtered through filter paper. Composition IX was prepared analogously to composition VIII, except that 1 g of zinc carbonate was used as the metal instead of copper carbonate. Compositions VIII and IX were then analysed for their antiprotozoal properties analogously to Example 1. Results were presented in Table 4.

TABLE-US-00004 TABLE 4 LD.sub.50, LD.sub.100 values for compositions VIII and IX, determined for selected protozoa. Sample Catalyst Copper carbonate Zinc carbonate Cedar oil Composition Composition Protozoa CH* M** Acid solution solution solution (Cedrus sp.) VIII IX Euglena LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: gracilis 0.05% LD.sub.100: 0.5% 0.05% 0.15% 0.1% 0.3% 0.03% 0.03% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1.1% 0.1% 0.25% 0.3% 0.4% 0.08% 0.08% Gregarina LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: blattarum LD.sub.100: 0.13% 0.9% 0.07% 0.12% 0.2% 0.1% 0.05% 0.055% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.3% 1.1% 0.3% 0.37% 0.4% 0.2% 0.08% 0.08% Amoeba LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: proteus 0.07% 0.3% 0.6% 0.05% 0.09% 0.15% 0.08% 0.03% 0.02% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 0.5% 1% 1% 0.17% 0.25% 0.1% 0.05% 0.05% Paramecium LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: caudatum 0.001% LD.sub.100: 0.8% 0.8% 0.35% 0.3 0.2% 0.07% 0.05% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.006% 1.25% 1.25% 0.5% 0.5% 0.3% 0.15% 0.17% Trichomanas LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: hominis LD.sub.100: 0.08% 0.8% 0.9% 0.9% 0.25% 0.25% 0.06% 0.07% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 1% 1.1% 1.1% 0.4% 0.4% 0.08% 0.08% *CH—chloramphenicol; **M—metronidazole

EXAMPLE 6

A Combination of Yarrow Oil (Achillea millefolium Linne) with a Mixture of Acids in a Ratio of 1:1 by Weight and with Copper or Zinc

[0112] In this non-limiting example, the following two compositions were prepared: [0113] a) composition X—i.e., a composition of yarrow oil with a mixture of acids and copper [0114] b) composition XI—i.e., a composition of yarrow oil with a mixture of acids and zinc carbonate

[0115] Although, in this non-limiting example, copper or zinc were used as a metal component, other metals such as molybdenum, cobalt, nickel, chromium, zinc, bismuth, copper, manganese, selenium, iron, their salts, or oxides can be used in the preparation of composition. In order to prepare composition X, 100 ml of an acid mixture (containing acetic, propionic, lactic and formic acids mixed in a ratio of 1:1:1:1), 0.6 g of catalyst (which is cobalt sulfate, ammonium molybdate and manganese chloride mixed in a ratio of 1:1:1) and 5 g of copper carbonate were added to 100 ml of yarrow oil. The mixture was heated at boiling point, until the colour changed, under reflux for 120 minutes. The mixture was then left to cool (for 24 hours) to obtain a clear solution (one, two or three phase solution). After this time, the reaction product was filtered through filter paper. Composition XI was prepared analogously to composition X, except that 5 g of zinc carbonate was used as the metal instead of copper carbonate. Compositions X and XI were then analysed for their antiprotozoal properties. Results were presented in Table 5. The results of the analysis showed that the killing and static activity in the compositions and after the reaction was higher than that of the individual substances in the reaction mixtures and complexes. All the ingredients used in the compositions are approved for both animal and human nutrition by the relevant directives and authorities, which, combined with their high efficacy, allows their use in the treatment and/or prevention of parasitoses in animals, caused by protozoa, in particular histomonadiasis (caused by Histomonas meleagridis), coccidiosis (caused by Eimeria), cryptosporidiosis (caused by Cryptosporidium), trichomonadiasis (caused by Trichomonas), babesiosis (caused by Babesia), or amoebiasis (caused by Amoeba).

TABLE-US-00005 TABLE 5 LD.sub.50, LD.sub.100 values for compositions X and XI, determined for selected protozoa. Sample Mixture of Catalyst Copper carbonate Zinc carbonate Yarrow oil Composition Composition Protozoa CH* M** acids solution solution solution (Achillea millefolium L.) X XI Euglena LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: gracilis 0.05% LD.sub.100: 0.5% 0.05% 0.15% 0.1% 0.08% 0.035% 0.025% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1.1% 0.1% 0.25% 0.3% 0.1% 0.055% 0.035% Gregarina LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: blattarum LD.sub.100: 0.13% 0.9% 0.07% 0.12% 0.2% 0.1% 0.025% 0.065% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.3% 1.1% 0.3% 0.37% 0.4% 0.2% 0.035% 0.075% Amoeba LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: proteus 0.07% 0.3% 0.6% 0.05% 0.09% 0.15% 0.09% 0.065% 0.045% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 0.5% 1% 1% 0.17% 0.25% 0.1% 0.075% 0.075% Paramecium LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: caudatum 0.001% LD.sub.100: 0.8% 0.8% 0.35% 0.3 0.1% 0.055% 0.075% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.006% 1.25% 1.25% 0.5% 0.5% 0.3% 0.065% 0.095% Trichomonas LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: hominis LD.sub.100: 0.08% 0.8% 0.9% 0.9% 0.25% 0.09% 0.025% 0.045% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 1% 1.1% 1.1% 0.4% 0.1% 0.035% 0.075% *CH—chloramphenicol; **M—metronidazole

EXAMPLE 7

A Combination of a Wormwood Oil (Artemisia absinthium Linne) with a Mixture of Acids in a Ratio of 1:1 by Weight and with Copper or Zinc

[0116] In this example following two compositions were prepared: [0117] a) composition XII—i.e., a composition of wormwood oil with a mixture of acids and copper [0118] b) composition XIII—i.e., a composition of wormwood oil with a mixture of acids and zinc

[0119] In order to prepare composition XII, 100 ml mixture of acids (containing acetic acid, propionic acid, lactic acid and formic acid mixed in a ratio of 1:1:1:1), 0.6 g of catalyst (which is cobalt sulfate, ammonium molybdate and manganese sulfate mixed in a ratio of 1:1:1) and 5 g copper carbonate were added to 100 ml of wormwood oil. The mixture was heated at boiling point, until the colour changed, under reflux for 90 minutes. The mixture was then left to cool (for 12 hours) to obtain a clear solution (one, two or three phase solution). After this time, the reaction product was filtered through filter paper.

[0120] Composition XIII was prepared analogously to composition XII, except that 5 g of zinc carbonate was added instead of copper carbonate. Compositions XII and XIII were then analysed for their antiprotozoal properties analogously to Example 1. Results are presented in Table 6.

[0121] The results obtained from the protozoal activity test are presented in Table 6. The results of the analysis showed that the killing and static activity in the compositions and after the reaction was higher than that of the individual substances in the reaction mixtures and complexes. All the ingredients used in the compositions are approved for both animal and human nutrition by the relevant directives and authorities, which, combined with their high efficacy, allows their use in the treatment and/or prevention of parasitoses in animals, caused by protozoa, in particular histomonadiasis (caused by Histomonas meleagridis), coccidiosis (caused by Eimeria), cryptosporidiosis (caused by Cryptosporidium), trichomonadiasis (caused by Trichomonas), babesiosis (caused by Babesia), or amoebiasis (caused by Amoeba).

TABLE-US-00006 TABLE 6 LD.sub.50, LD.sub.100 values for compositions XII and XIII, determined for selected protozoa. Sample Mixture of Catalyst Copper carbonate Zinc carbonate Wormwood oil (Artemisia Composition Composition Protozoa CH* M** acids solution solution solution absinthium Linne) XII XIII Euglena LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: gracilis 0.05% LD.sub.100: 0.5% 0.05% 0.15% 0.1% 0.1% 0.004% 0.002% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1.1% 0.1% 0.25% 0.3% 0.3% 0.005% 0.004% Gregarina LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: blattarum LD.sub.100: 0.13% 0.9% 0.07% 0.12% 0.2% 0.1% 0.002% 0.004% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.3% 1.1% 0.3% 0.37% 0.4% 0.2% 0.005% 0.006% Amoeba LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: proteus 0.07% 0.3% 0.6% 0.05% 0.09% 0.15% 0.1% 0.002% 0.005% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 0.5% 1% 1% 0.17% 0.25% 0.2% 0.004% 0.007% Paramecium LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: caudatum 0.001% LD.sub.100: 0.8% 0.8% 0.35% 0.3% 0.4% 0.006% 0.003% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.006% 1.25% 1.25% 0.5% 0.5% 0.5% 0.007% 0.005% Trichomonas LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: hominis LD.sub.100: 0.08% 0.8% 0.9% 0.9% 0.25% 0.09% 0.001% 0.004% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 1% 1.1% 1.1% 0.4% 0.1% 0.002% 0.007% *CH—chloramphenicol; **M—metronidazole

EXAMPLE 8

A Combination of Origanum Oil (Origanum vulgare L.) with a Mixture of Acids in a Ratio of 1:1 by Weight and with Copper or Zinc

[0122] In this example the following two compositions were prepared: [0123] a) composition XIV—i.e., a composition of Origanum oil with a mixture of acids and copper [0124] b) composition XV—i.e., a composition of Origanum oil with a mixture of acids and zinc

[0125] In order to prepare composition XIV, 100 ml mixture of acids (containing acetic acid, propionic acid, lactic acid and formic acid mixed in a ratio of 1:1:1:1), 0.6 g of catalyst (which is cobalt sulfate, ammonium molybdate and manganese sulfate mixed in a ratio of 1:1:1) and 5 g copper carbonate were added to 100 ml of Origanum oil. The mixture was heated at the boiling point, until the color changed, under reflux for 20 minutes. The mixture was then left to cool (for 10 hours) to obtain a clear solution (one, two or three phase solution). After this time, the reaction product was filtered through filter paper.

[0126] Composition XV was prepared analogously to composition XIV, except that 5 g of zinc carbonate was added instead of copper carbonate. Compositions XIV and XV were then analysed for their antiprotozoal properties analogously to Example 1. Results are presented in Table 7.

TABLE-US-00007 TABLE 7 LD.sub.50, LD.sub.100 values for compositions XIV and XV, determined for selected protozoa. Sample Mixture of Catalyst Copper carbonate Zinc carbonate Origanum Composition Composition Protozoa CH* M** acids solution solution solution oil XIV XV Euglena LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: gracilis 0.05% LD.sub.100: 0.5% 0.05% 0.15% 0.1% 0.5% 0.035% 0.03% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1.1% 0.1% 0.25% 0.3% 0.6% 0.07% 0.06% Gregarina LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: blattarum LD.sub.100: 0.3% 0.9% 0.07% 0.12% 0.2% 0.4% 0.05% 0.03% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.3% 1.1% 0.3% 0.37% 0.4% 0.5% 0.08% 0.04% Amoeba LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: proteus 0.07% 0.3% 0.6% 0.05% 0.09% 0.15% 0.5% 0.04% 0.03% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 0.5% 1% 1% 0.17% 0.25% 0.7% 0.09% 0.08% Paramecium LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: caudatum 0.001% LD.sub.100: 0.8% 0.8% 0.35% 0.3% 0.4% 0.1% 0.09% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.006% 1.25% 1.25% 0.5% 0.5% 0.5% 0.3% 0.25% Trichomonas LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: hominis LD.sub.100: 0.08% 0.8% 0.9% 0.9% 0.25% 0.2% 0.01% 0.05% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 1% 1.1% 1.1% 0.4% 0.3% 0.06% 0.07% *CH—chloramphenicol; **M—metronidazole

EXAMPLE 9

A Combination of a Lavender Oil (Lavandula angustifolia Miller) with a Mixture of Acids in a Ratio of 1:1 by Weight and with Copper or Zinc

[0127] In this non-limiting example, lavender oil was used to prepare the compositions. However, another essential oil selected from the group consisting of: cedar oil, tea oil, cardamom oil, wormwood oil, cubeb oil, eucalyptus oil, mint oil, juniper oil, cypress oil, lemongrass oil, spruce oil, pine oil, fir oil, Douglas fir oil, caraway oil, cumin oil, patchouli oil, sage oil, Inula oil, tansy oil, angelica oil calamus oil, ginger oil, thyme oil, Origanum oil, rosemary oil, nutmeg oil, coriander oil, geranium oil, carrot seed oil, yarrow herb or flower oil can be used in composition according to invention as an essential oil component

[0128] In this example the following two compositions were prepared: [0129] a) composition XVI—i.e., a composition of lavender oil with a mixture of acids and copper [0130] b) composition XVII—i.e., a composition of lavender oil with a mixture of acids and zinc

[0131] Wherein, in order to prepare composition XVI, 100 ml mixture of acids (containing acetic acid, propionic acid, lactic acid and formic acid mixed in a ratio of 1:1:1:1), 0.3 g of catalyst (which is cobalt sulfate, ammonium molybdate and manganese sulfate mixed in a ratio of 1:1:1) and 5 g copper carbonate were added to 100 ml of lavender oil. The mixture was heated at boiling point, until the colour changed, under reflux for 120 minutes. The mixture was then left to cool (for 24 hours) to obtain a clear solution (one, two or three phase solution). After this time, the reaction product was filtered through filter paper.

[0132] Composition XVII was prepared analogously to composition XVI, except that 5 g of zinc carbonate was added instead of copper carbonate. Compositions XVI and XVII were then analysed for their antiprotozoal properties analogously to Example 1. Results are presented in Table 8. The results of the analysis showed that the killing and static activity in complex systems and after the reaction was higher than that of the substances separately, included in the reaction mixtures and complexes.

TABLE-US-00008 TABLE 8 LD.sub.50, LD.sub.100 values for compositions XVI and XVII, determined for selected protozoa. Sample Mixture of Catalyst Copper carbonate Zinc carbonate Lavender oil (Lavandula Composition Composition Protozoa CH* M** acids solution solution solution angustifolia Miller) XVI XVII Euglena LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: gracilis 0.05% LD.sub.100: 0.5% 0.05% 0.15% 0.1% 0.1% 0.025% 0.025% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.1% 1.1% 0.1% 0.25% 0.3% 0.2% 0.045% 0.055% Gregarina LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: blattarum LD.sub.100: 0.13% 0.9% 0.07% 0.12% 0.2% 0.1% 0.055% 0.055% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.3% 1.1% 0.3% 0.37% 0.4% 0.2% 0.075% 0.085% Amoeba LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: proteus 0.07% 0.3% 0.6% 0.05% 0.09% 0.15% 0.1% 0.025% 0.025% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 0.5% 1% 1% 0.17% 0.25% 0.2% 0.055% 0.065% Paramecium LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: caudatum 0.001% LD.sub.100: 0.8% 0.8% 0.35% 0.3% 0.1% 0.025% 0.045% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.006% 1.25% 1.25% 0.5% 0.5% 0.2% 0.045% 0.065% Trichomonas LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: LD.sub.50: hominis LD.sub.100: 0.08% 0.8% 0.9% 0.9% 0.25% 0.1% 0.055% 0.085% LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: LD.sub.100: 0.15% 1% 1.1% 1.1% 0.4% 0.2% 0.075% 0.15% *CH—chloramphenicol; **M—metronidazole