Method for the preparation of an anti-streptococcus serum for use in the treatment of Coronavirus-related diseases.

Abstract

An antistreptococcal serum for the treatment of respiratory and other diseases associated with Covid-19 and other Coronaviruses derived from the blood of a male foal of an ass less than 2 years old which has been prepared through successive incubations such as to render an otherwise fatal dose of streptococci innocuous.

Claims

1. An antistreptococcal serum derived in accordance with the method detailed in the Detailed description of the Invention contained in the Specification relating to application Ser. No. 16/951,906 and which is obtained from the blood of: 1. A male ass; and 2. which is younger than 2 years old.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0014] 1). Antistreptococcal serum is obtained from the blood of a colt, the foal of an ass (being a male ass not older than two years), which has received during several months repeated and increasing injections of living virulent streptococci.

[0015] 2). Antistreptococcal serum may be expected to possess antimycotic rather than anti-toxic properties.

[0016] 3). Ordinary laboratory streptococcus is practically useless for the production of an active serum.

[0017] 4). Cultures obtained from various sources failed altogether to give satisfactory results until their virulence had been re-enforced by successive passages through susceptible animals.

[0018] 5). Each injection of streptococci was always followed by considerable local and general reaction, characterized principally by local swelling and rise of body temperature. Such disturbances usually continued during several days, but ultimately a complete recovery took place.

[0019] 6). Tests applied to the serum obtainable after each injection showed a steady increase of its antimycotic powers. Such tests are carried out as follows: The lethal dose of a standard streptococcus culture having been ascertained, similar quantities of culture are mixed in vitro with various proportions of a). normal serum from a colt less than two years old, b). the serum to be tested. On injecting these mixtures into animals of nearly equal weights it is readily demonstrable that while the control mixtures are almost uniformly fatal, those containing the prepared serum in proper proportion are practically innocuous. A maximum of one-hundredth of a cubic centimetre of serum should neutralize an otherwise lethal dose of streptococci.

[0020] 7). It has been demonstrated that streptococci may remain in the blood of an animal for several days after their introduction. To ensure their absence from serum intended for clinical use, it is therefore imperative to remove all chance microbes by filtration through porcelain. In actual practice Chamberlain filters are used, and the serum is made to pass through them by pressure furnished by liquid carbonic acid gas. The actual pressure need seldom exceed one of about 70 lbs. to the square inch.