Compound composition with function of promoting bone and joint health and application thereof

Abstract

The present invention discloses a compound composition with function of promoting bone and joint health and an application thereof. In the present invention, glucosamine is combined with collagen, chondroitin sulfate and traditional Chinese medicine extract to prepare the compound composition with function of promoting bone and joint health. The compound composition can shorten onset time. At the same time, the traditional Chinese medicine extract for treating the bone and joint problems is added, to reduce the market impact of adding only modern joint health ingredients. Moreover, the compound composition disclosed by the present invention can be used as a dietary supplement or health food raw material for preventing or treating moderate to mild osteoarthritis. Thus, the compound composition disclosed by the present invention is suitable for promotion and application.

Claims

1. A compound composition with function of promoting bone and joint health, wherein the compound composition is composed of the following raw materials in parts by weight: TABLE-US-00015 glucosamine 1.0-3.0 parts; collagen 0.1-1.0 part; chondroitin sulfate 0.1-1.0 part; and traditional Chinese medicine extract 0.1-1.0 part; wherein the traditional Chinese medicine extract is a mixture composed of at least two extracts of an epimedium brevicornu extract, a salviae miltiorrhizae extract, a dioscorea nipponica makino extract, a rhizoma drynariae extract and a dodder extract; wherein the traditional Chinese medicine extract is prepared by extracting traditional Chinese medicine; wherein the traditional Chinese medicine comprises the following components in parts by weight: 7-12 parts of epimedium brevicornu, 0.5-3 parts of salviae miltiorrhizae, 6.5-9 parts of dioscorea nipponica makino, 1-5 parts of rhizoma drynariae and 1-5 parts of dodder.

2. The compound composition with function of promoting bone and joint health according to claim 1, wherein the compound composition is composed of the following raw materials in parts by weight: TABLE-US-00016 glucosamine 1.0-1.5 parts; collagen 0.2-0.7 part; chondroitin sulfate 0.1-0.3 part; and traditional Chinese medicine extract 0.1-0.5 part.

3. The compound composition with function of promoting bone and joint health according to claim 2, wherein the compound composition is composed of the following raw materials in parts by weight: TABLE-US-00017 glucosamine 1.2 parts; collagen 0.4 part; chondroitin sulfate 0.2 part; and traditional Chinese medicine extract 0.35 part.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) To more clearly describe the technical solution in the embodiments of the present invention or in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be simply presented below. Apparently, the drawings in the following description are merely the embodiments of the present invention, and for those ordinary skilled in the art, other drawings can also be obtained according to the provided drawings without contributing creative labor.

(2) FIG. 1 is a diagram of a pain scoring standard of the present invention.

DETAILED DESCRIPTION

(3) The technical solution disclosed by the present invention will be clearly and fully described below in combination with the embodiments of the present invention. Apparently, the described embodiments are merely part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those ordinary skilled in the art without contributing creative labor will belong to the protection scope of the present invention.

(4) Embodiments of the present invention disclose a compound composition having rapid effect, small daily dosage and low cost. The compound composition has the function of promoting bone and joint health, and can be used as a dietary supplement or health food raw material for preventing or treating moderate to mild osteoarthritis.

(5) To better understand the present invention, the present invention is further described in detail below by the following embodiments, but shall not be interpreted as a limitation to the present invention. Some non-essential improvements and adjustments made by those skilled in the art according to the contents of the present invention shall also be deemed to fall within the protection scope of the present invention.

(6) The present invention discloses a compound composition with function of promoting bone and joint health. The compound composition is composed of the following raw materials in parts by weight:

(7) TABLE-US-00004 glucosamine 1.0-3.0 parts; collagen 0.1-1.0 part; chondroitin sulfate 0.1-1.0 part; traditional Chinese medicine extract 0.1-1.0 part.

(8) To further optimize the above technical solution, the traditional Chinese medicine extract is prepared by extracting traditional Chinese medicine, and the traditional Chinese medicine extract is a mixture composed of at least two extracts of an epimedium brevicornu extract, a salviae miltiorrhizae extract, a dioscorea nipponica makino extract, a rhizoma drynariae extract and a dodder extract.

(9) To further optimize the above technical solution, the traditional Chinese medicine comprises the following components in parts by weight: 7-12 parts of epimedium brevicornu, 0.5-3 parts of salviae miltiorrhizae, 6.5-9 parts of dioscorea nipponica makino, 1-5 parts of rhizoma drynariae and 1-5 parts of dodder.

(10) The present invention also discloses an application of the compound composition with function of promoting bone and joint health in preparation of health food.

(11) The technical solution disclosed by the present invention is further described below in combination with specific embodiments.

Embodiment 1

(12) A compound composition with function of promoting bone and joint health is provided. The compound composition is composed of the following raw materials in parts by weight:

(13) TABLE-US-00005 glucosamine 1.2 parts; collagen 0.9 part; chondroitin sulfate 0.8 part; traditional Chinese medicine extract 0.2 part.

Embodiment 2

(14) A compound composition with function of promoting bone and joint health is provided. The compound composition is composed of the following raw materials in parts by weight:

(15) TABLE-US-00006 glucosamine 2.8 parts; collagen 0.2 part; chondroitin sulfate 0.5 part; traditional Chinese medicine extract 0.5 part.

Embodiment 3

(16) A compound composition with function of promoting bone and joint health is provided. The compound composition is composed of the following raw materials in parts by weight:

(17) TABLE-US-00007 glucosamine 1.5 parts; collagen 0.7 part; chondroitin sulfate 0.3 part; traditional Chinese medicine extract 0.6 part.

Embodiment 4

(18) A compound composition with function of promoting bone and joint health is provided. The compound composition is composed of the following raw materials in parts by weight:

(19) TABLE-US-00008 glucosamine 2.5 parts; collagen 0.5 part; chondroitin sulfate 0.3 part; traditional Chinese medicine extract 0.35 part.

Embodiment 5

(20) A compound composition with function of promoting bone and joint health is provided. The compound composition is composed of the following raw materials in parts by weight:

(21) TABLE-US-00009 glucosamine 1.2 parts; collagen 0.4 part; chondroitin sulfate 0.2 part; traditional Chinese medicine extract 0.35 part.

Embodiment 6

(22) The traditional Chinese medicine used by the traditional Chinese medicine extract in the compound composition with function of promoting bone and joint health disclosed by any of the above embodiments 1-5 comprises the following components in parts by weight:

(23) 8 parts of epimedium brevicornu, 3 parts of salviae miltiorrhizae, 9 parts of dioscorea nipponica makino, 2 parts of rhizoma drynariae and 4 parts of dodder.

(24) The preparation method of the traditional Chinese medicine extract is as follows:

(25) extracting each flavor of medicine with an alcohol extraction technology, purifying with macroporous resin; concentrating and spray-drying to finally obtain the traditional Chinese medicine extracts.

Embodiment 7

(26) The traditional Chinese medicine used by the traditional Chinese medicine extract in the compound composition with function of promoting bone and joint health disclosed by any of the above embodiments 1-5 comprises the following components in parts by weight:

(27) 10 parts of epimedium brevicornu, 2 parts of salviae miltiorrhizae, 8 parts of dioscorea nipponica makino, 4 parts of rhizoma drynariae and 2 parts of dodder.

(28) The preparation method of the traditional Chinese medicine extract is as follows:

(29) extracting each flavor of medicine with an alcohol extraction technology; purifying with macroporous resin; concentrating and spray-drying to finally obtain the traditional Chinese medicine extracts.

Embodiment 8

(30) The traditional Chinese medicine used by the traditional Chinese medicine extract in the compound composition with function of promoting bone and joint health disclosed by any of the above embodiments 1-5 comprises the following components in parts by weight:

(31) 12 parts of epimedium brevicornu, 0.8 part of salviae miltiorrhizae, 7.2 parts of dioscorea nipponica makino, 3 parts of rhizoma drynariae and 3 parts of dodder.

(32) The preparation method of the traditional Chinese medicine extract is as follows:

(33) extracting each flavor of medicine with an alcohol extraction technology; purifying with macroporous resin; concentrating and spray-drying to finally obtain the traditional Chinese medicine extracts.

(34) Specifically, the preparation method of the above compound composition with function of promoting bone and joint health comprises the following steps: (1) extracting each flavor of medicine with an alcohol extraction technology; purifying with macroporous resin; concentrating and spray-drying to obtain the traditional Chinese medicine extracts; (2) uniformly mixing the traditional Chinese medicine extracts obtained in the above preparation with the glucosamine, the collagen and the chondroitin sulfate to obtain the compound composition.

(35) Exemplarily, as seen below, embodiment 9 to embodiment 11 disclose a preparation method of the compound composition with function of promoting bone and joint health.

Embodiment 9

(36) A compound composition with function of promoting bone and joint health is composed of the following raw materials in parts by weight: 100 g of glucosamine, 30 g of collagen, 10 g of chondroitin sulfate and traditional Chinese medicine extract which is obtained by extracting 700 g of epimedium brevicornu medicine, 100 g of salviae miltiorrhizae medicine and 700 g of dioscorea nipponica makino medicine.

(37) The preparation method of the compound composition comprises the following specific steps:

(38) (1) adding 8 times the amount of 70% ethanol to the epimedium brevicornu medicine; conducting heating reflux extraction twice for 1.5 h each time; merging the extracting solutions; purifying; concentrating; adding 10% β-cyclodextrin; spray-drying at 200° C.; and filtering with a 80-mesh sieve to obtain an epimedium brevicornu extract;

(39) (2) adding 8 times the amount of 80% ethanol to the salviae miltiorrhizae medicine; conducting heating reflux extraction twice for 1.5 h each time; merging the extracting solutions; purifying; concentrating; adding 10% β-cyclodextrin; spray-drying at 200′C; and filtering with a 80-mesh sieve to obtain a salviae miltiorrhizae extract;

(40) (3) adding 8 times the amount of 70% ethanol to the dioscorea nipponica makino medicine; conducting heating reflux extraction three times for 1.5 h each time; merging the extracting solutions; purifying; concentrating; adding 10% β-cyclodextrin; spray-drying at 200′C; and filtering with a 80-mesh sieve to obtain a dioscorea nipponica makino extract;

(41) (4) mixing the traditional Chinese medicine extracts obtained in the above preparation, and uniformly mixing with the glucosamine, the collagen and the chondroitin sulfate to obtain the compound composition of 100 times prescribed daily dosage.

Embodiment 10

(42) A compound composition with function of promoting bone and joint health is composed of the following raw materials in parts by weight: 240 g of glucosamine, 40 g of collagen, 60 g of chondroitin sulfate and traditional Chinese medicine extract which is obtained by extracting 2000 g of epimedium brevicornu medicine, 600 g of rhizoma drynariae medicine and 600 g of dodder medicine.

(43) The preparation method of the compound composition comprises the following specific steps:

(44) (1) adding 10 times the amount of 70% ethanol to the epimedium brevicornu medicine; conducting heating reflux extraction twice for 2 h each time; merging the extracting solutions; purifying; concentrating; adding 15% β-cyclodextrin; spray-drying at 200° C.; and filtering with a 80-mesh sieve to obtain an epimedium brevicornu extract;

(45) (2) adding 20 times the amount of 80% ethanol to the rhizoma drynariae medicine; conducting heating reflux extraction twice for 1.5 h each time; merging the extracting solutions; purifying; concentrating; adding 10% β-cyclodextrin; spray-drying at 200° C.; and filtering with a 80-mesh sieve to obtain a rhizoma drynariae extract;

(46) (3) adding 6 times the amount of 75% ethanol to the dodder medicine; conducting heating reflux extraction twice for 2 h each time; merging the extracting solutions; purifying; concentrating; adding 10% β-cyclodextrin; spray-drying at 200° C.; and filtering with a 80-mesh sieve to obtain a dodder extract;

(47) (4) mixing the traditional Chinese medicine extracts obtained in the above preparation, and uniformly mixing with the glucosamine, the collagen and the chondroitin sulfate to obtain the compound composition of 200 times prescribed daily dosage.

Embodiment 11

(48) A compound composition with function of promoting bone and joint health is composed of the following raw materials in parts by weight: 150 g of glucosamine, 50 g of collagen, 60 g of chondroitin sulfate and traditional Chinese medicine extract which is obtained by extracting 2000 g of epimedium brevicornu medicine and 100 g of salviae miltiorrhizae medicine.

(49) The preparation method of the compound composition comprises the following specific steps:

(50) (1) adding 10 times the amount of 70% ethanol to the epimedium brevicornu medicine; conducting heating reflux extraction twice for 2 h each time; merging the extracting solutions; purifying; concentrating; adding 15% β-cyclodextrin; spray-drying at 200° C.; and filtering with a 80-mesh sieve to obtain an epimedium brevicornu extract;

(51) (2) adding 10 times the amount of 80% ethanol to the salviae miltiorrhizae medicine; conducting heating reflux extraction twice for 2 h each time; merging the extracting solutions; purifying; concentrating; adding 10% β-cyclodextrin; spray-drying at 200′C; and filtering with a 80-mesh sieve to obtain a salviae miltiorrhizae extract;

(52) (3) mixing the traditional Chinese medicine extracts obtained in the above preparation, and uniformly mixing with the glucosamine, the collagen and the chondroitin sulfate to obtain the compound composition of 100 times prescribed daily dosage.

(53) Each embodiment in the description is described in a progressive way. The difference of each embodiment from each other is the focus of explanation. The same and similar parts among all of the embodiments can be referred to each other.

(54) The contents of the present invention are not limited to the contents of the above embodiments, and a combination of one or more embodiments can also achieve the purposes of the present invention.

(55) In order to further verify the excellent effects of the present invention, the inventors also conduct the following experiment.

(56) Test I:

(57) Function of the compound composition with function of promoting bone and joint health in anti-inflammation.

(58) Male wistar rats are randomly divided into a control group, a model group and an experimental group. Each group includes 10 rats. The rats are injected with papain for molding. The rats are administered for 28 days at the same time of molding. Before the rats are put to death, blood is taken from the abdominal vein and centrifuged to obtain serum. The IL-1β content is detected. Specific results are shown in Table 1:

(59) TABLE-US-00010 TABLE 1 Levels of Inflammatory Factor IL- 1β in Serums of Rats in Each Group Number of Daily dosage IL-1β content Groups animals (piece) mg/kg (ng/L) Blank group 12 Equal-volume 5.063 ± 0.721 normal saline Model group 12 Equal-volume  8.08 ± 1.176 normal saline Experimental 12   169.2  6.573 ± 0.6719* administration group Glucosamine 12 126 9.456 ± 1.657 sulfate control group 1 Glucosamine + 12 207  8.975 ± 0.7605 chondroitin sulfate + collagen control group 2

(60) The blank group includes normal rats for arthritis modeling. The model group includes rats not administered after modeling. The experimental administration group administers the product of the present invention to the rats. The glucosamine sulfate control group administers glucosamine to the rats. The glucosamine+chondroitin sulfate+collagen control group administers glucosamine+chondroitin sulfate+collagen to the rats. IL-1β is an inflammatory factor in the serum. Normal rats contain the lowest levels of inflammatory factors, and the rats with arthritis but not administered contain the highest levels of inflammatory factors. If different products are administered to rats, the degree of arthritis is alleviated to a certain extent and the levels of inflammatory factors in the rats are reduced to be lower than that of the highest model group, but higher than normal rats. It can be seen from the data in Table 1 that the level of the inflammatory factor of the experimental administration group, i.e., the product group of the present invention, is obviously different from that of the model group, and is lower than those of the control group 1 and the control group 2, which proves that the product has the function of anti-inflammation and has better effect than the single glucosamine and the combination of the glucosamine and the pharmaceutical ingredients.

(61) In addition, to verify the technical solution of the present invention and small daily dosage of the single glucosamine, the inventor respectively experiments the male wistar rats in the same administration time and under the same administration dosage, and detects the IL-6 content in the serum. Specific results are shown in Table 2:

(62) TABLE-US-00011 TABLE 2 Levels of Inflammatory Factor IL-6 in Serums of Rats in Each Group Number of Daily dosage IL-6 content Groups animals (piece) mg/kg (ng/L) Blank group 10 Equal-volume 102.03 ± 6.78  normal saline Model group 10 Equal-volume 158.79 ± 17.53 normal saline Experimental 10 125   142.59 ± 17.17 * administration group Glucosamine 10 125 146.19 ± 18.15 sulfate control group

(63) It can be seen from the data in Table 2 that in the same administration time and under the same administration dosage, in reducing the inflammatory factor IL-6, the experiment administration group is obviously lower than the model group. The glucosamine sulfate control group is not obviously lower than the model group. The inflammatory factor content in the serum in the experimental administration group is lower than that in the glucosamine sulfate group, which indicates that the formula product has advantages over the single glucosamine in terms of anti-inflammation, proving that the same effect can be achieved with less dosage.

(64) Test II:

(65) Function of the compound composition with function of promoting bone and joint health in pain alleviation.

(66) Male wistar rats are randomly divided into a control group, a model group and an experimental group. Each group includes 10 rats. The rats are injected with papain for molding. The rats are administered for 28 days at the same time of molding. Before the rats are put to death, the tenderness thresholds at modeling joints are measured with a tenderness meter. Specific results are shown in Table 3:

(67) TABLE-US-00012 TABLE 3 Results of Tenderness Thresholds of Rats in Each Group Tenderness Number of Daily dosage thresholds Groups animals (piece) mg/kg (newton) Blank group 12 Equal-volume 6.348 ± 0.2191 normal saline Model group 12 Equal-volume 5.681 ± 0.2264 normal saline Experimental 12   169.2 6.212 ± 0.1607 administration group Glucosamine 12 126 5.607 ± 0.2602 sulfate control group 1 Glucosamine + 12 207 5.922 ± 0.1346 chondroitin sulfate + collagen control group 2

(68) Normal rats in the blank group do not suffer from arthritis, and their joints can withstand larger forces when pressed. The rats in the model group do not receive treatment of arthritis, are sensitive to pain, and withstand smaller forces when pressed. The rats administered with the product can alleviate the arthritis to a certain extent, and withstand larger forces than the model group when pressed. It can be seen from the data in Table 3 that the experimental administration group withstand larger forces than the model group, proving the function of alleviating arthritis pain, and also withstand larger forces than the control group 1 and the control group 2, proving that the product has the function of alleviating the tenderness and has better effect than the single glucosamine and the combination of the glucosamine and the pharmaceutical ingredients.

(69) In addition, the applicant tests the influences of different traditional Chinese medicine combinations on the tenderness thresholds of the rats. Specific results and analysis are shown in test III.

(70) Test III:

(71) Male wistar rats are randomly divided into a model group, an experimental administration group 1, an experimental administration group 2 and an experimental administration group 3. Each group includes 10 rats. The rats are injected with papain for molding. The rats are administered for 28 days at the same time of molding. Before the rats are put to death, the tenderness thresholds at modeling joints are measured with a tenderness meter. Specific results are shown in Table 4: In the experimental administration group 1, the rats are administered with the product of the present invention prepared by the combination of three kinds of traditional Chinese medicine of epimedium brevicornu, salviae miltiorrhizae and dioscorea nipponica makino. In the experimental administration group 2, the rats are administered with the product of the present invention prepared by the combination of two kinds of traditional Chinese medicine of dodder and rhizoma drynariae. In the experimental administration group 3, the rats are administered with the product of the present invention prepared by the combination of five kinds of traditional Chinese medicine of epimedium brevicornu, salviae miltiorrhizae, dioscorea nipponica makino, dodder and rhizoma drynariae.

(72) TABLE-US-00013 TABLE 4 Influences of Different Traditional Chinese Medicine Combinations on Tenderness Thresholds of Rats Tenderness Number of Daily dosage thresholds Groups animals (piece) mg/kg (kPa) Blank group 10 Equal-volume 450.03 ± 6.78  normal saline Model group 10 Equal-volume 208.79 ± 17.53  normal saline Experimental 10 55   292.59 ± 17.17 ** administration group 1 Experimental 10 50 264.19 ± 18.15* administration group 2 Experimental 10 75 281.64 ± 16.44* administration group 3

(73) Normal rats in the blank group do not suffer from arthritis, and their joints can withstand larger forces when pressed. The rats in the model group do not receive treatment of arthritis, are sensitive to pain, and withstand smaller forces when pressed. The rats administered with the product can alleviate the arthritis to a certain extent, and withstand larger forces than the model group when pressed. It can be seen from the data in Table 4 that the experimental administration group withstand larger forces than the model group, proving the function of alleviating arthritis pain. Meanwhile, the tenderness threshold of the experimental administration group 1 is larger than those of the experimental administration group 2 and the experimental administration group 3, thereby proving that addition of formula traditional Chinese medicine in the product has the function of alleviating the arthritis pain and different traditional Chinese medicine combinations have different effects.

(74) Test IV:

(75) Function of the compound composition with function of promoting bone and joint health in pain alleviation.

(76) Male wistar rats are randomly divided into a control group, a model group and an experimental group. Each group includes 10 rats. The rats are injected with papain for molding. The rats are administered for 28 days at the same time of molding. Before the rats are put to death, the left and right soles of the rats are evenly imprinted with printing ink of the same color and the rats walk by 0.4 m on a self-made semi-closed rat walking passage. The sole marks are photographed. Three persons independently score the walking pain based on the sole photos according to the following scoring standard for walking pain and measure the walking pain (when the limbs have a pain reaction, the rats move with the minimum contact area of the paws to reduce the pressure on the affected limbs; and when the left and right feet are uneven in stress and the gait is changed, the colors of the soles are different).

(77) The scoring standard for the pain is shown in FIG. 1. The sole is divided into four regions: if one region of the left sole is darker than the right sole, the score is 1; if two regions are darker, the score is 2; and the score is added in sequence (0-4 scores).

(78) Specific results are shown in Table 5:

(79) TABLE-US-00014 TABLE 5 Results of Walking Pain of Rats in Each Group Number of Daily dosage Scores of Groups animals (piece) mg/kg walking pain Blank group 12 Equal-volume 0.08333 ± 0.08333 normal saline Model group 12 Equal-volume 0.6364 ± 0.1521 normal saline Experimental 12   169.2 0.45 ± 0.25 administration group Glucosamine 12 126   0.5 ± 0.2303 sulfate control group 1 Glucosamine + 12 207 0.5833 ± 0.2876 chondroitin sulfate + collagen control group 2

(80) Normal rats in the blank group do not suffer from arthritis, and their feet can normally touch the ground with force when walking. The rats in the model group do not receive treatment of arthritis, are sensitive to pain, and do not dare to force the feet when walking. The rats administered with the product can alleviate the arthritis to a certain extent, and can walk slightly normally, so as to reflect the degree of arthritis according to the walking scores. It can be seen from the data in Table 5 that the pain score of the experimental administration group is less than that of the model group, proving the function of alleviating arthritis pain, and also less than those of the control group 1 and the control group 2, proving that the product has the function of alleviating the walking pain and has better effect than the single glucosamine and the combination of the glucosamine and the pharmaceutical ingredients.

(81) Each embodiment in the description is described in a progressive way. The difference of each embodiment from each other is the focus of explanation. The same and similar parts among all of the embodiments can be referred to each other. For a method disclosed by the embodiments, because the method corresponds to a method disclosed by the embodiments, the method is simply described. Refer to the description of the method part for the related part.

(82) The above description of the disclosed embodiments enables those skilled in the art to realize or use the present invention. Many modifications to these embodiments will be apparent to those skilled in the art. The general principle defined herein can be realized in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principle and novel features disclosed herein.