Cleaning compositions comprising dispersin variants
11732220 · 2023-08-22
Assignee
Inventors
- Mirko WEIDE (Duesseldorf, DE)
- Susanne Wieland (Zons/Dormagen, DE)
- Lars Henrik Oestergaard (Bagsvaerd, DK)
- Annette Helle Johansen (Bagsvaerd, DK)
- Steffen Danielsen (Bagsvaerd, DK)
- Roland Alexander Pache (Bagsvaerd, DK)
- Johanne Moerch Jensen (Bagsvaerd, DK)
- Frank Winther Rasmussen (Bagsvaerd, DK)
- Martin Gudmand (Bagsvaerd, DK)
- Kasper Damgaard Tidemand (Bagsvaerd, DK)
Cpc classification
C11D3/386
CHEMISTRY; METALLURGY
C12N9/2402
CHEMISTRY; METALLURGY
C11D3/38636
CHEMISTRY; METALLURGY
International classification
C11D3/386
CHEMISTRY; METALLURGY
C11D9/44
CHEMISTRY; METALLURGY
Abstract
Cleaning compositions may include novel dispersin variants having improved properties relative to the parent dispersin. Such properties may include one or more of the following; wash performance, stability e.g. detergent and/or storage stability. The cleaning compositions may be suitable for use in cleaning processes and include detergent compositions, such as laundry compositions and dish wash compositions, including hand wash and automatic dish wash compositions.
Claims
1. A cleaning composition comprising a dispersin variant, said dispersin variant comprising a substitution at one or more positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323 of the polypeptide of SEQ ID NO: 1, wherein the dispersin variant has beta-1,6 N-acetylglucosaminidase activity, wherein the dispersin variant has at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1, and wherein the variant has increased stability compared to the dispersin of SEQ ID NO: 1, wherein said cleaning composition: (a) is a solid laundry detergent composition and further comprises: (a1) at least one zeolite builder; (a2) at least one phosphonate builder; (a3) at least one further enzyme; and (a4) at least one polymer; or (b) is a solid laundry detergent composition and further comprises: (b1) at least one silicate builder; (b2) at least one further enzyme; and (b3) at least one bleaching system, comprising a bleaching agent, a bleach activator, and a bleach catalyst; or (c) is a liquid laundry detergent composition and further comprises: at least one surfactant; or (d) is a liquid laundry detergent in unit dose form, and further comprises: water in an amount of up to 20 wt; or (e) is a fabric finisher and further comprises: (e1) at least one softening silicone; (e2) at least one perfume; or (f) is an acidic cleaning agent, and further comprises: (f1) plant-based or bio-based surfactants; (f2) at least one acidic biocide; and (f3) at least one soil release, water repellant or water spreading polymer; or (g) is a neutral cleaning agent, and further comprises: (g1) plant-based or bio-based surfactants; (g2) at least one biocide; and (g3) at least one soil release, water repellant or water spreading polymer; or (h) is an alkaline cleaning agent, and further comprises: (h1) plant-based or bio-based surfactants; or (i) is a hand dishwashing agent, and further comprises: (i1) at least one anionic surfactant; (i2) at least one amphoteric surfactant; (i3) at least one nonionic surfactant; (i4) at least one further enzyme; or (j) is an automatic dishwashing composition and further comprises: (j1) at least one builder selected from citrate, aminocarboxylates and combinations thereof; (j2) at least one phosphonate builder; (j3) at least one nonionic surfactant; (j4) at least one bleaching system, comprising a bleaching agent, a bleach activator and a bleach catalyst; and (j5) at least one polymer selected from sulfopolymers, cationic polymers and polyacrylates; or (k) further comprises: (k1) at least one sulfopolymer; or (l) further comprises at least one adjunct ingredient selected from probiotics, spores or combinations thereof; or (m) is in unit dose form and comprises at least 2 separate compartments; or is a phosphate-free composition.
2. The cleaning composition of claim 1, wherein said variant comprises at least one alteration selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181N, S181P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R; wherein the positions correspond to amino acid positions in the amino acid sequence set forth in SEQ ID NO: 1; and wherein the substitution at the one or more positions provides a dispersin variant having an increase in stability, HIF, of at least 1.1; and further wherein the variants have at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1.
3. The cleaning composition according to claim 1, wherein the variant is obtained from Terribacillus.
4. The cleaning composition according to claim 1, wherein the variant comprises one or more motifs: GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14).
5. The cleaning composition according to claim 1, wherein the dispersin variant has improved stability, measured as having an improvement factor RAR or HIF>1.0, compared to the dispersin having the polypeptide sequence shown in SEQ ID NO: 1 or shown in SEQ ID NO: 15.
6. The cleaning composition according to claim 1, wherein the Half-life Improvement Factor (HIF) is at least 1.2, compared to SEQ ID NO: 1 or compared to SEQ ID NO: 15, wherein HIF is calculated as T.sub.1/2dispersinvariant/T.sub.1/2reference and the half-life (T.sub.1/2 in minutes) for the dispersin variants and the dispersin reference of SEQ ID NO: 1 or 15 is calculated as: 20 minutes×LN(0.5)/LN(RA) and the residual activity (RA) for each dispersin variant and the reference dispersin of SEQ ID NO: 1 or 15 is calculated as: slope (stress sample, 56° C. for 60 min)/slope(unstressed sample, 21° C. for 60 min).
7. The cleaning composition according to claim 1, wherein in the variant the total number of substitutions compared to SEQ ID NO: 1 ranges from 3 to 20 substitutions compared to the dispersin comprising the amino acid sequence shown in SEQ ID NO: 1.
8. A method of cleaning an item comprising: providing a cleaning composition according to claim 1; and washing item with the composition, wherein the item is a textile or a hard surface.
Description
DETAILED DESCRIPTION
(1) Cleaning compositions, as defined herein, may include novel dispersins preferably obtained from Terribacillus, in particular, Terribacillus saccharophilus. The dispersins preferably comprise at least 60% sequence identity to a polypeptide with SEQ ID NO: 1 and comprise an alteration of at least one amino acid positions compared to the dispersin with SEQ ID NO: 1. In some aspects, a dispersin variant comprises an amino acid sequence comprising at least two substitutions of an amino acid made at positions equivalent to the positions in SEQ ID NO: 1. Disclosed are also methods for producing dispersin variants. The dispersin variants preferably have at least one improved property compared to the parent dispersin or compared to SEQ ID NO: 1. Properties include but are not limited to: stability such as; stability in detergents, storage stability, in wash stability and thermo-stability, wash performance in particular deep-cleaning performance, increased expression level and malodor reduction. Preferably the improved property is improved stability e.g. stability in detergent (e.g. cleaning compositions). When household care surfaces such as textiles are soiled the stain is often a complex mixture of various organic matters such as skin debris e.g. dead cells, sweat and sebum, pollution from outside and from e.g. the body. Such biosoil composes different organic stains such as protein, starch, grease but also e.g. polysaccharides. Such complex stains are often stubborn and difficult to remove. The use of dispersins in laundry processes and in detergents has been described in WO2017/186943 (Novozymes A/S), which disclose the dispersins from Terribacillus such as dispersins with SEQ ID NO 1. Dispersins have shown very effective in removing e.g. PNAG (Poly-N-Acetyl Glucosamine) related stains from textiles under laundry conditions. However, when enzymes are formulated into cleaning compositions, such as detergents, they may be less active over time, particularly in liquid detergents. Obviously, its preferable that enzymes are stable in cleaning and detergent compositions during storage (storage stability) and during the wash process (in-wash stability). The former may be measured in accelerated stability tests where the dispersin variants are tested under stressed (e.g. increased heating) and non-stressed conditions, residual activity (RA) or Half-life (T.sub.1/2) are measured and compared to the values of the parent (or another reference). The residual activity ratio (RAR) or Half-life improvement factor (HIF) of the reference or parent dispersin may be assigned the value 1. Thus, dispersin variants having RAR or HIF above 1 have improved stability under the measured conditions compared to the parent or reference dispersin. In an embodiment, the dispersin variants comprised in the compositions have improved stability compared to the parent dispersin and are therefore advantageous over state-of-the-art dispersins.
(2) Dispersin Variants
(3) Some aspects relate to cleaning compositions as defined herein comprising dispersin variants of SEQ ID NO: 1 or variants of a dispersin having at least 60% identity hereto.
(4) A cleaning composition as defined herein may include a dispersin comprising an alteration at one or more position selected from the list consisting of positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(5) A cleaning composition as defined herein may include a dispersin comprising an alteration at one or more position selected from the list consisting of positions 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(6) A cleaning composition as defined herein may include a dispersin comprising an alteration at one or more positions selected from the list consisting of positions 2, 3, 12, 15, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 221, 225, 227, 232, 235, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322 and 323, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(7) A cleaning composition as defined herein may include a dispersin comprising an alteration at one or more positions selected from the list consisting of positions 3, 15, 49, 59, 140, 163, 186, 207, 215, 218, 225, 227, 232, 235, 237, 252, 260, 267, 272, 276, 279, 281, 288, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(8) A cleaning composition as defined herein may include a dispersin comprising a substitution at one or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(9) A cleaning composition as defined herein may include a dispersin comprising a substitution at two or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(10) A cleaning composition as defined herein may include a dispersin comprising a substitution at three or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(11) A cleaning composition as defined herein may include a dispersin comprising a substitution at four or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(12) A cleaning composition as defined herein may include a dispersin comprising a substitution at five or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(13) A cleaning composition as defined herein may include a dispersin comprising a substitution at six or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(14) A cleaning composition as defined herein may include a dispersin comprising a substitution at seven or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(15) A cleaning composition as defined herein may include a dispersin comprising a substitution at eight or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(16) A cleaning composition as defined herein may include a dispersin comprising a substitution at nine or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(17) A cleaning composition as defined herein may include a dispersin comprising a substitution at ten or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(18) A cleaning composition as defined herein may include a dispersin comprising a substitution at eleven or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(19) A cleaning composition as defined herein may include a dispersin comprising a substitution at twelve or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(20) A cleaning composition as defined herein may include a dispersin comprising a substitution at thirteen or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(21) A cleaning composition as defined herein may include a dispersin comprising a substitution at fourteen or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(22) A cleaning composition as defined herein may include a dispersin comprising a substitution at fifthteen or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(23) A cleaning composition as defined herein may include a dispersin comprising a substitution at sixteen or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(24) A cleaning composition as defined herein may include a dispersin comprising a substitution at seventeen or more positions selected from the list consisting of positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(25) A cleaning composition as defined herein may include a dispersin comprising a substitution at all the positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent e.g. compared to SEQ ID NO 1.
(26) The term “comprising a substitution” is in the present context meant comprising a substitution compared to the starting dispersin or the parent. The thus, dispersin have a replacement of the amino acid in e.g. position 3 with another amino acid.
(27) In some aspects the variant has a sequence identity of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, to the amino acid sequence of the mature parent dispersin e.g. the dispersin shown in SEQ ID NO: 1.
(28) In some preferred aspects, the dispersin variant comprises an alteration is selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181N, S181P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein the variant has dispersin activity and wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(29) In some preferred aspects the alteration is a substitution and the dispersin variant comprises one or more substitution(s) selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43H, N43L, N45D, N45L, N45V, A49W, A49Y, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117D, S117H, S117N, S117P, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181N, S181P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261A, E261D, E261R, E261W, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein the variant has dispersin activity and wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(30) One aspect relates to a cleaning composition as defined herein comprising a dispersin variant, which compared to a dispersin with SEQ ID NO: 1, comprises one or more substitutions selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43H, N43L, N45D, N45L, N45V, A49W, A49Y, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117D, S117H, S117N, S117P, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122H, D122R, Y123W, Y124C, Y124H, Y124I, Y124K, Y124L, Y124M, Y124N, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125H, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128A, V128C, V128D, V128L, V128T, D131V, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138N, D138Q, D138R, D138S, D138V, D138W, E139W, V140I, D142R, D142W, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150A, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, S163P, G164D, G164E, G164H, G164S, G164V, V167A, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181K, S181N, S181P, S181Q, S181T, S181V, S181W, E185A, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203Q, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207K, D207N, D207Q, D207R, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215K, Q215R, Q215M, Q215L, S217V, T218A, T218L, T218Q, T218R, T218V, S221N, G222D, E224A, E224P, S225G, N227A, N227Q, N227R, N227S, N227T, N227K, D230N, D230R, D230T, D230W, E232D, E232V, N233D, N233E, N233H, N233Q, N233R, N233W, W234R, G235A, G235W, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251A, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256D, Q256E, Q256R, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260T, N260V, N260W, N260Y, E261A, E261D, E261R, E261W, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264C, D264E, D264N, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288P, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308E, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309E, K309G, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312E, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, Y321F and N323R, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to SEQ ID NO 1, and wherein the variant has beta-1,6 N-acetylglucosaminidase activity.
(31) One aspect relates to a cleaning composition as defined herein comprising a dispersin variant, which compared to a dispersin with SEQ ID NO: 1, comprises one or more substitutions selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43H, N43L, N45D, N45L, N45V, A49W, A49Y, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117D, S117H, S117N, S117P, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181N, S181P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261A, E261D, E261R, E261W, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to SEQ ID NO 1, and wherein the variant has beta-1,6 N-acetylglucosaminidase activity.
(32) In some preferred aspects the alteration is a substitution and the dispersin variant comprises one or more substitutions selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A and N323R, wherein the variant has dispersin activity preferably beta-1,6 N-acetylglucosaminidase activity and wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(33) In some aspects the variant has a sequence identity of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, to the amino acid sequence of the mature parent dispersin e.g. the dispersin shown in SEQ ID NO: 1.
(34) In some aspects, the number of substitutions in the variants is 2-20, e.g., 2-10 and 2-5, such as 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 substitutions. In one aspect the total number of substitutions compared to SEQ ID NO: 1 is 3-20, e.g. at least 5 to 20, at least 10 to 20 or such as at least 15 to 20 substitutions compared to the dispersin comprising the amino acid sequence shown in SEQ ID NO: 1.
(35) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 2 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 2 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Leu, Ala, Trp, Arg or Asn. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of D2V, D2L, D2A, D2W, D2R and D2N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(36) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 3 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 3 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe, Leu, Val, Tyr, Met, Ile, Pro or Thr. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of Q3F, Q3L, Q3V, Q3Y, Q3M, Q3I, Q3P and Q3T, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(37) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 12 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 12 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala. In another aspect, the dispersin variant comprises or consists of the substitution S12A, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(38) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 15 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 15 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Tyr or Phe. In another aspect, the dispersin variant comprises or consists of the substitution H15Y or H15F, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(39) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 17 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 17 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Val, Arg, Met, Phe, Cys or Glu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of T17W, T17V, T17R, T17M, T17F, T17C and T17E, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(40) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 18 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 18 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu. In another aspect, the dispersin variant comprises or consists of the substitution V18L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(41) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 19 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 19 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro, Asn, Lys or Asp. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of E19P, E19N, E19K and E19D, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(42) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 22 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 22 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Val or Met. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of K22A, K22V and K22M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(43) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 23 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 23 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Cys, Glu, Ile, Leu, Arg, Thr or Val. In another aspect, the dispersin variant comprises or consists of the substitution selected from the group consisting of S23A, S23C, S23E, S23I, S23L, S23R, S23T and S23V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(44) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 24 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 24 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution L24I, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(45) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 25 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 25 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg. In another aspect, the dispersin variant comprises or consists of the substitution V25R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(46) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 26 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 26 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met. In another aspect, the dispersin variant comprises or consists of the substitution D26M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(47) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 30 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 30 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 30 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Val, Asp, Thr, Asn, Met, Ala or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of Y30R, Y30V, Y30D, Y30T, Y30N, Y30M, Y30A and Y30L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(48) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 32 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 32 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Met or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of G32R, G32M and G32L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(49) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 34 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 34 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp. In another aspect, the dispersin variant comprises or consists of the substitution N34D, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(50) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 43 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 43 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 43 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu or His. In another aspect, the dispersin variant comprises or consists of the substitution N43L or N43H, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(51) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 44 of SEQ ID NO 1.
(52) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 45 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 45 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Leu or Val. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of N45D, N45L and N45V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(53) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 49 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 49 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp or Tyr. In another aspect, the dispersin variant comprises or consists of the substitution A49W, A49Y, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(54) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 52 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 52 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 52 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met. In another aspect, the dispersin variant comprises or consists of the substitution Y52M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(55) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 54 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 54 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Leu or Met. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of G54N, G54L and G54M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(56) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 56 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 56 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp or Thr. In another aspect, the dispersin variant comprises or consists of the substitution S56W or S56T, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(57) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 57 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 57 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp. In another aspect, the dispersin variant comprises or consists of the substitution S57W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(58) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 58 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 58 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution E58N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(59) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 59 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 59 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Cys, Asp, Glu, Phe, Met, Arg, Val or Trp. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(60) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 60 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 60 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution T60V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(61) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 62 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 62 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Gln, His, Cys or Asp. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of N62W, N62Q, N62H, N62C and N62D, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(62) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 63 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 63 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Leu, Asn, Arg, Asp or Cys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of T63V, T63L, T63N, T63R, T63D and T63C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(63) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 67 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 67 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu or Ala. In another aspect, the dispersin variant comprises or consists of the substitution K67L or K67A, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(64) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 68 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 68 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu or Gln. In another aspect, the dispersin variant comprises or consists of the substitution N68L or N68Q, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(65) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 71 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 71 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, His, Asn, Trp, or Val. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of L71R, L71H, L71N, L71W and L71V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(66) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 72 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 72 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 72 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr, Gly, Cys, Met, Phe, Asn, Tyr, Arg, Ile, Asp or Glu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of S72T, S72G, S72C, S72M, S72F, S72N, S72Y, S72R, S72I, S72D and S72E, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(67) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 74 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 74 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu. In another aspect, the dispersin variant comprises or consists of the substitution I74L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(68) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 77 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 77 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala. In another aspect, the dispersin variant comprises or consists of the substitution S77A, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(69) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 79 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 79 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution D79V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(70) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 80 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 80 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 80 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Arg, Trp, Leu, Val, Gln or His. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of K80N, K80R, K80W, K80E, K80L, K80V, K80Q and K80H, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(71) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 81 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 81 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly, Ala, Trp, Leu, Trp, Thr, Val, Arg, Ser or Asn. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of D81G, D81A, D81L, D81W, D81T, D81V, D81R, D81S and D81N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(72) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 82 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 82 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution I82V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(73) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 90 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 90 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe. In another aspect, the dispersin variant comprises or consists of the substitution L90F, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(74) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 99 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 99 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln or Arg. In another aspect, the dispersin variant comprises or consists of the substitution E99Q or E99R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(75) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 100 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 100 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ser. In another aspect, the dispersin variant comprises or consists of the substitution L100S, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(76) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 103 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 103 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Val or Ala. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of K103R, K103V and K103A, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(77) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 104 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 104 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn or Trp. In another aspect, the dispersin variant comprises or consists of the substitution K104N or K104W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(78) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 105 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 105 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution D105N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(79) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 106 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 106 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Tyr, Leu, His, Glu, Trp, Asn, Arg, Met, Asp, Lys, Ala or Gln. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of V106Y, V106L, V106H, V106E, V106W, V106N, V106R, V106M, V106D, V106K, V106A and V106Q, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(80) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 107 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 107 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met, Trp, Leu, Cys, Val, Ala or Thr. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of K107M, K107W, K107L, K107C, K107V, K107A and K107T, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(81) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 110 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 110 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Val or Met. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: N110R, N110V and N110M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(82) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 111 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 111 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Ala, Arg, Asn, Trp, Met, Gln or Glu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: D111V, D111A, D111R, D111N, D111W, D111M, D111Q and D111E, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(83) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 113 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 113 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr. In another aspect, the dispersin variant comprises or consists of the substitution V113T, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(84) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 114 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 114 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ser or Cys. In another aspect, the dispersin variant comprises or consists of the substitution T114S or T114C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(85) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 116 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 116 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Asn or Arg. In another aspect, the dispersin variant comprises or consists of the substitution Y116D, Y116N or Y116R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(86) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 117 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 117 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 117 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, His, Asn or Pro. In another aspect, the dispersin variant comprises or consists of the substitution S117D, S117H, S117N or S117P, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(87) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 118 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 118 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 118 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Asp, Gly or Leu. In another aspect, the dispersin variant comprises or consists of the substitution E118A, E118D, E118G or E118L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(88) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 119 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 119 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly or Trp. In another aspect, the dispersin variant comprises or consists of the substitution E119G or E119W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(89) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 120 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 120 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile, Leu, Met, Val or Trp. In another aspect, the dispersin variant comprises or consists of the substitution T120I, T120L, T120M, T120V or T120W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(90) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 122 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 122 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 122 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg or His. In another aspect, the dispersin variant comprises or consists of the substitution D122R or D122H, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(91) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 123 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 123 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp. In another aspect, the dispersin variant comprises or consists of the substitution Y123W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(92) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 124 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 124 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile, Met, His, Arg, Val, Gln, Thr, Lys, Leu, Asn, Trp or Cys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: Y124I, Y124M, Y124H, Y124R, Y124V, Y124Q, Y124T, Y124K, Y124L, Y124N, Y124W and Y124C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(93) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 125 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 125 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Cys, Gly, His, Lys or Gln. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: D125R, D125C, D125G, D125H, D125K and D125Q, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(94) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 126 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 126 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution N126V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(95) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 127 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 127 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Trp, Gln, Met, Asp, His or Lys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: R127L, R127W, R127Q, R127M, R127D, R127H and R127K, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(96) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 128 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 128 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Asp, Ala, Cys, or Thr. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: V128L, V128D, V128A, V128C and V128T, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(97) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 131 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 131 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution D131V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(98) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 135 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 135 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 135 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met, Tyr, Ala, Asp, Glu or Lys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: Q135M, Q135Y, Q135A, Q135D, Q135E and Q135K, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(99) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 138 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 138 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met, Val, Leu, Trp, Ser, Arg, Lys, Asn or Gln. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: D138M, D138V, D138L, D138W, D138S, D138R, D138K, D138N and D138Q, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(100) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 139 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 139 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp. In another aspect, the dispersin variant comprises or consists of the substitution E139W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(101) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 140 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 140 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution V140I, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(102) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 142 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 142 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp or Arg. In another aspect, the dispersin variant comprises or consists of the substitution D142W or D142R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1
(103) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 145 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 145 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 145 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Asn, His or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: Y145V, Y145N, Y145H and Y145L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(104) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 147 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 147 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Asp, Ala, Val, Gly, Arg, Ser, Met, Thr, Cys or Phe. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: P147L, P147D, P147A, P147V, P147G, P147R, P147S, P147M, P147T, P147C and P147F, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(105) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 148 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 148 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Asp, Ala or Val. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: K148L, K148D, K148A and K148V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(106) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 149 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 149 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Met or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: F149N, F149M and F149L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(107) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 150 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 150 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Arg, Asp, Lys, Mat, His, Trp, Val, Gly, Leu or Ala. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: E150N, E150R, E150D, E150K, E150M, E150H, E150W, E150V, E150Y, E150L and E150A, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(108) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 151 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 151 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Pro, Trp, Asp, Leu, Ser, Asn or Cys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: G151A, G151P, G151W, G151D, G151L, G151S, G151N and G151C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(109) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 152 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 152 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Leu or Arg. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: K152D, K152L and K152R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(110) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 163 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 163 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro. In another aspect, the dispersin variant comprises or consists of the substitution S163P, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1
(111) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 164 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 164 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, His, Ser, Glu or Val. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: G164D, G164H, G164S, G164E and G164V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(112) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 167 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 167 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Ala, Trp, Pro, Asp, Leu, Arg or Glu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: V167Q, V167A, V167W, V167P, V167D, V167L, V167R and V167E, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(113) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 168 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 168 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution H168N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1
(114) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 170 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 170 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Lys, Ser, His, Asn, Arg, Glu, Phe, Met, Ala, Trp, Tyr, Val, Gln or Pro. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: L170D, L170K, L170S, L170H, L170N, L170R, L170E, L170F, L170M, L170A, L170W, L170Y, L170V, L170Q and L170P, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(115) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 171 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 171 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Ala, Trp, Arg, Cys, Glu, Lys, Tyr, Met, Gln or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: D171V, D171A, D171W, D171R, D171C, D171E, D171K, D171Y, D171M, D171Q and D171L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(116) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 173 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 173 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Cys. In another aspect, the dispersin variant comprises or consists of the substitution I173C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1
(117) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 174 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 174 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, His, Met, Asn or Val. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: D174W, D174H, D174M, D174N and D174V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(118) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 175 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 175 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Tyr. In another aspect, the dispersin variant comprises or consists of the substitution F175Y, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(119) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 177 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 177 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met. In another aspect, the dispersin variant comprises or consists of the substitution N177M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(120) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 178 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 178 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 178 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Trp, Ala or Lys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: Q178R, Q178W, Q178A and Q178K, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(121) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 179 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 179 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr. In another aspect, the dispersin variant comprises or consists of the substitution I179T, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(122) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 181 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 181 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr, Phe, Gln, Gly, Asn, Cys, Lys, Trp, Val, Asp, Thr or Pro. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: S181T, S181F, S181Q, S181G, S181N, S181C, S181K, S181W, S181V, S181D, S181T and S181P, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(123) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 185 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 185 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Arg, Met, Val or Trp. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: E185A, E185R, E185M, E185V and E185W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(124) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 186 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 186 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Glu, Asn, Gln, Leu, Trp, Val, Ile, Lys, Met, Arg or His. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: S186D, S186E, S186N, S186Q, S186L, S186W, S186V, S186I, S186K, S186M, S186R and S186H, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(125) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 187 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 187 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly, Arg, Trp, Val, Cys, Asp or Ser. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: K187G, K187R, K187W, K187V, K187C, K187D and K187S, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(126) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 188 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 188 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro. In another aspect, the dispersin variant comprises or consists of the substitution Y188P, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(127) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 189 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 189 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with, Leu, Val or Trp. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: E189V, E189L and E189W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(128) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 199 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 199 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Tyr, Met, Cys or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: S199Y, S199M, S199C and S199L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(129) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 200 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 200 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Asp, Lys, Arg, Met, Phe, Asn or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: E200W, E200D, E200K, E200R, E200M, E200F, E200N and E200L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(130) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 203 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 203 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Thr, Pro, Cys, Leu, Ser, Met, Gln, Thr, Asp, Gly, Glu or Val. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: A203R, A203W, A203P, A203C, A203L, A203S, A203M, A203Q, A203T, A203D, A203G, A203E and A203V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(131) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 204 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 204 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Tyr, Val, Trp or Met. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: N204L, N204Y, N204V, N204W and N204M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(132) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 205 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 205 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution L205I, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(133) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 207 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 207 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Lys, Ser, Cys, Gly, Asn, Val, Arg, Gln, Glu, Trp or Ala. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: D207S, D207C, D207G, D207N, D207V, D207R, D207Q, D207E, D207W, D207K and D207A, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(134) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 208 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 208 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Val, Cys, Trp, Asp, Thr, Gln, Gly or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: S208A, S208V, S208C, S208W, S208D, S208T, S208Q, S208G and S208L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(135) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 210 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 210 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr. In another aspect, the dispersin variant comprises or consists of the substitution S210T, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(136) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 215 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 215 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 215 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Lys, Arg, Leu or Met. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: Q215K, Q215R, Q215L and Q215M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(137) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 217 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 217 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution S217V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(138) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 218 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 218 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Arg, Ala, Val or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: T218Q, T218R, T218A, T218V and T218L, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(139) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 221 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 221 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution S221N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(140) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 222 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 222 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp. In another aspect, the dispersin variant comprises or consists of the substitution G222D, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(141) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 224 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 224 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala or Pro. In another aspect, the dispersin variant comprises or consists of the substitution E224A or E224P, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(142) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 225 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 225 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly. In another aspect, the dispersin variant comprises or consists of the substitution S225G, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(143) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 227 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 227 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Gln, Arg, Ser, Thr or Lys. In another aspect, the dispersin variant comprises or consists of the substitution N227A, N227Q, N227R, N227S, N227T or N227K, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(144) In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at a position corresponding to position 230 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 230 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 230 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Arg, Trp or Thr. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: D230N, D230R, D230W and D230T, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(145) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 232 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 232 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp or Val. In another aspect, the dispersin variant comprises or consists of the substitution E232D or E232V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(146) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 233 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 233 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Asp, His, Gln, Arg or Trp. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: N233E, N233D, N233H, N233Q, N233R and N233W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(147) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 234 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 234 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg. In another aspect, the dispersin variant comprises or consists of the substitution W234R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(148) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 235 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 235 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Ala, Ser, Val, Leu, Met, Asn, His, Glu, Phe, Pro or Ile. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: G235W, G235A, G235S, G235V, G235L, G235M, G235N, G235H, G235E, G235F, G235P and G235I, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(149) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 237 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 237 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Tyr, Gly, Met, Asn or Cys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: S237W, S237Y, S237G, S237M, S237N and S237C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(150) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 244 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 244 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Met or Cys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: Y244E, Y244M and Y244C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(151) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 249 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 249 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Arg, Trp, His, Lys or Tyr. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of: L249Q, L249R, L249W, L249H, L249K and L249Y, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(152) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 251 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 251 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Leu, Arg, Trp or Asn. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of S251A, S251L, S251R, S251W and S251N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(153) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 252 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 252 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro or Cys. In another aspect, the dispersin variant comprises or consists of the substitution N252P or N252C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(154) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 253 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 253 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp or Trp. In another aspect, the dispersin variant comprises or consists of the substitution G253D, or G253W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(155) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 254 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 254 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile, Leu, Met or Tyr. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of F254I, F254L, F254M and F254Y, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(156) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 256 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 256 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Arg or Asp. In another aspect, the dispersin variant comprises or consists of the substitution Q256E, Q256R or Q256D, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(157) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 260 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 260 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 260 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Leu, Cys, Arg, Met, Lys, Ala, Val, Ile, Thr, Glu, Trp or Tyr. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of N260Q, N260L, N260C, N260R, N260M, N260K, N260A, N260V, N260I, N260T, N260E, N260W and N260Y, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(158) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 261 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 261 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Asp, Arg or Trp. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of E261A, E261D, E261R and E261W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(159) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 262 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 262 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 262 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with His, Phe, Trp or Tyr. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of Q262H, Q262F, Q262W and Q262Y, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(160) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 263 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 263 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Leu or Lys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of M263Q, M263L and M263K, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(161) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 264 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 264 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 264 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Cys or Asn. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of D264E, D264C and D264N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(162) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 265 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 265 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe. In another aspect, the dispersin variant comprises or consists of the substitution Y265F, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(163) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 267 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 267 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr or Ser. In another aspect, the dispersin variant comprises or consists of the substitution N267T or N267S, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(164) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 268 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 268 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Arg, Cys or Met. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of W268E, W268R, W268C and W268M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(165) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 270 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 270 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe. In another aspect, the dispersin variant comprises or consists of the substitution Y270F, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(166) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 271 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 271 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly or Asp. In another aspect, the dispersin variant comprises or consists of the substitution A271G or A271D, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(167) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 272 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 272 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Met, Pro, Ile, Val, or Trp. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of H272D, H272M, H272P, H272I, H272V, and H272W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(168) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 273 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 273 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp. In another aspect, the dispersin variant comprises or consists of the substitution N273W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(169) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 274 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 274 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with His, Arg or Ala. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of K274H, K274R and K274A, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(170) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 276 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 276 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Asn, Lys, Cys, Gly, Leu, Met, Pro, Ser, Val or Trp. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of F276A, F276N, F276K, F276C, F276G, F276L, F276M, F276P, F276S, F276V and F276W, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(171) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 278 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 278 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Gln, Ala, Asn or Lys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of I278V, I278Q, I278A, I278N and I278K, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(172) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 279 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 279 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Cys, Glu, Asn, Asp or Gly. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of S279C, S279E, S279N, S279D and S279G, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(173) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 280 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 280 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu or Cys. In another aspect, the dispersin variant comprises or consists of the substitution D280E or D280C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(174) In another aspect, the dispersin variant comprises or consists of a deletion of the amino acid at a position corresponding to position 281 of SEQ ID NO 1. In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 281 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 281 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with His, Ala, Lys, Asn, Cys, Pro, or Arg. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of Y281H, Y281A, Y281K, Y281N, Y281C, Y281P, and Y281R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(175) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 282 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 282 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn or Glu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of Y282N and Y282E, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(176) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 283 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 283 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution H283I, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(177) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 284 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 284 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Ile, Val, Asn, Pro or Thr. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of A284L, A284I, A284V, A284N, A284P and A284T, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(178) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 287 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 287 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution T287N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(179) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 288 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 288 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro, Asp, Asn or Lys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of S288P, S288D, S288N and S288K, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(180) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 290 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 290 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution V290I, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(181) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 291 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 291 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Leu or Val. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of K291R, K291L and K291V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(182) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 296 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 296 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Cys. In another aspect, the dispersin variant comprises or consists of the substitution T296C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(183) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 300 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 300 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp or Ala. In another aspect, the dispersin variant comprises or consists of the substitution E300D or E300A, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(184) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 301 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 301 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Cys or Arg. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of H301N, H301C and H301R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(185) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 303 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 303 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Ala, Cys, Lys, Gly, Trp or Arg. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of T303Q, T303A, T303C, T303K, T303G, T303W and T303R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(186) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 304 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 304 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met or Cys. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of D304M and D304C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(187) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 305 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 305 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met or Asn. In another aspect, the dispersin variant comprises or consists of the L305M or L305N, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(188) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 306 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 306 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Cys. In another aspect, the dispersin variant comprises or consists of the S306C, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(189) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 308 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 308 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Glu, Ala, Val, Gln, Ser, Tyr, Gly, Leu, Thr or Ile. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of K308D, K308E, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T and K308I, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(190) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 309 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 309 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Gly, Cys, Leu, Asp, Gln, Asn, Thr, Ala, Ser, Met, His or Ile. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of K309E, K309G, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H and K309I, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(191) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 312 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 312 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Ala, Gln, Ser, Trp, Leu, Asn or Met. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of K312E, K312A, K312Q, K312S, K312W, K312L, K312N and K312M, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(192) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 314 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 314 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Val or Ile. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of E314L, E314V and E314I, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(193) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 315 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 315 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile or Val. In another aspect, the dispersin variant comprises or consists of the L315I or L315V, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(194) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 319 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 319 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala. In another aspect, the dispersin variant comprises or consists of the R319A, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(195) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 321 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 321 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe. In another aspect, the dispersin variant comprises or consists of the Y321F, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(196) In another aspect, the dispersin variant comprises or consists of a substitution at a position corresponding to position 323 of SEQ ID NO 1. In another aspect, the amino acid at a position corresponding to position 323 of SEQ ID NO 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg. In another aspect, the dispersin variant comprises or consists of the N323R, wherein the position corresponds to the positions of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence shown in SEQ ID NO 1.
(197) In one aspect, the variant comprises or consists of one or more the substitutions at a position selected from the group consisting of: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein the position corresponds to the positions of SEQ ID NO: 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to the polypeptide shown in SEQ ID NO: 1 which has beta-1,6 N-acetylglucosaminidase activity, and further the variant has at least one improved property compared to a reference enzyme e.g. compared to SEQ ID NO 1.
(198) In one aspect, the variant comprises or consists of one or more the substitutions at a position selected from the group consisting of: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 218, 221, 225, 227, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322 and 323, wherein the position corresponds to the positions of SEQ ID NO: 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to the polypeptide shown in SEQ ID NO: 1 which has beta-1,6 N-acetylglucosaminidase activity, and further the variant has at least one improved property compared to a reference enzyme e.g. compared to SEQ ID NO 1.
(199) In one aspect, the variant comprises or consists of one or more the substitutions at a position selected from the group consisting of: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein the position corresponds to the positions of SEQ ID NO: 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to the polypeptide shown in SEQ ID NO: 1 which has dispersin, preferably beta-1,6 N-acetylglucosaminidase activity, and further the variant has at least one improved property compared to a reference enzyme e.g. compared to SEQ ID NO 1.
(200) In one aspect, the variant comprises or consists of one or more of the alterations selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124H, Y124I, Y124K, Y124L, Y124M, Y124N, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125H, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128A, V128C, V128D, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138N, D138Q, D138R, D138S, D138V, D138W, E139W, V140I, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150A, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, S163P, G164D, G164E, G164H, G164S, G164V, V167A, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181K, S181N, S181P, S181Q, S181T, S181V, S181W, E185A, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203Q, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207K, D207N, D207Q, D207R, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215K, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, S221N, G222D, E224A, E224P, S225G, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230N, D230R, D230T, D230W, E232D, E232V, N233D, N233E, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251A, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256D, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260T, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, D264N, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288P, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308E, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309E, K309G, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312E, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, Y321F and N323R, wherein the position corresponds to the positions of SEQ ID NO: 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to the polypeptide shown in SEQ ID NO: 1 which has beta-1,6 N-acetylglucosaminidase activity, and further the variant has at least one improved property compared to a reference enzyme e.g. compared to SEQ ID NO 1.
(201) In one aspect, the variant comprises or consists of one or more of the substitutions selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252P, N252C, Q256E, Q262H, N260Q, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A and N323R, wherein the position corresponds to the positions of SEQ ID NO: 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to the polypeptide shown in SEQ ID NO: 1 which has dispersin, preferably beta-1,6 N-acetylglucosaminidase activity, and further the variant has at least one improved property compared to a reference enzyme e.g. compared to SEQ ID NO 1 or compared to SEQ ID NO:15.
(202) In some aspects, the improved property is increased stability e.g. improved detergent stability, improved in-wash stability and improved thermostability. Some aspects relate to cleaning compositions as defined herein comprising dispersin variants having an improvement factor above 1 (e.g. measured as HIF), when the dispersin variants are tested for a property of interest in a relevant assay, wherein the property of the reference dispersin is given a value of 1. In some aspects, the property is stability, such as storage stability. Some aspects relate to cleaning compositions as defined herein comprising dispersin variants having an improvement factor above 1 (e.g. measured as HIF), when the dispersin variants are tested for a property of interest in the assay described in Example 2, 3, 4, 5 or 6, wherein the property of the reference dispersin is given a value of 1. In some aspects, the property is stability, such as storage stability.
(203) In some aspects, the improved property is increased stability e.g. improved detergent stability, improved in-wash stability and improved thermostability. Some aspects relate to cleaning compositions as defined herein comprising dispersin variants having an improvement factor above 1 when the dispersin variants are tested for a property of interest in a relevant assay, wherein the property of the reference dispersin is given a value of 1. In some aspects, the property is stability, such as storage stability.
(204) In some aspects, the improved property is improved detergent stability.
(205) In some aspects, the improved property is improved protease stability.
(206) In some aspects a variant is improved under the measured conditions when the residual activity ratio, defined as
(207)
is above 1 compared to the reference dispersin, as shown in Examples 2a and 3. In some aspects, the variant is improved compared to the reference dispersin.
(208) In some aspects, the variants have improved stability relative to a reference dispersin measured as a residual activity ratio (RAR) that is greater than 1.0.
(209) The Residual Activity Ratio (RAR) for the dispersin variants may be calculated as RA.sub.variant/RA.sub.reference. Improved variants were identified as variants having residual activity ratio (RAR) larger than 1.0 compared to the reference dispersin, as shown in Example 2a and 3.
(210) In some aspects, the variants have improved stability relative to a reference dispersin measured as an Half-life Improvement Factor (HIF) that is greater than 1.0.
(211) The Half-life Improvement Factor (HIF) for the dispersin variants may be calculated as T.sub.1/2variant/T.sub.1/2reference. Improved variants were identified as variants having a Half-life Improvement Factor HIF larger than 1.0 compared to the reference dispersin, as shown in Example 2b. Improved alterations e.g. substitutions are those which result in an improvement factor e.g. HIF or RAR above 1 or is a variant which have at least one improved property compared to the starting molecule i.e. the precursor, reference parent polypeptide etc. In a particular preferred aspect, dispersin variants comprising the alterations results in dispersin variants having improved stability where HIF>1.0. In some aspects, the variants have a Half-life Improvement Factor (HIF) which is at least 1.1; 1.2; 1.3; 1.4; 1.5; 1.6; 1.7; 1.8; 1.9; 2.0; 2.1; 2.2; 2.3; 2.4; 2.5; 2.6; 2.7, 2.8; 2.9; 3.0, 3.1; 3.2; 3.3; 3.4; 3.5, 3.6, 3.7, 3.8, 3.9; 4.0, 4.1; 4.2; 4.3; 4.4; 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1; 5.2; 5.3; 5.4; 5.5, 5.6, 5.7, 5.8, 5.9; 3.0, 6.1; 6.2; 6.3; 6.4; 6.5, 6.6, 6.7, 6.8, 6.9; 7.0, 7.1; 7.2; 7.3; 7.4; 7.5, 7.6, 7.7, 7.8, 7.9; 8.0, 8.1; 8.2; 8.3; 8.4; 8.5, 8.6, 8.7, 8.8, 8.9; 9.0, 9.1; 9.2; 9.3; 9.4; 9.5, 9.6, 9.7, 9.8, 9.9; 10.0, 10.1; 10.2; 10.3; 10.4; 10.5, 10.6, 10.7, 10.8, 10.9; 12, 15, 16, 20, 25 or 30 compared to a reference dispersin e.g. SEQ ID NO: 1 or compared to SEQ ID NO 15.
(212) In a preferred aspect, the combination of mutations according to the present disclosure results in dispersin variants having improved stability, where RAR>1.0. In some aspects, the variants have a Residual Activity Ratio (RAR) which is at least 1.1; 1.2; 1.3; 1.4; 1.5; 1.6; 1.7; 1.8; 1.9; 2.0; 2.1; 2.2; 2.3; 2.4; 2.5; 2.6; 2.7, 2.8; 2.9; 3.0, 3.1; 3.2; 3.3; 3.4; 3.5, 3.6, 3.7, 3.8, 3.9; 4.0, 4.1; 4.2; 4.3; 4.4; 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1; 5.2; 5.3; 5.4; 5.5, 5.6, 5.7, 5.8, 5.9; 3.0, 6.1; 6.2; 6.3; 6.4; 6.5, 6.6, 6.7, 6.8, 6.9; 7.0, 7.1; 7.2; 7.3; 7.4; 7.5, 7.6, 7.7, 7.8, 7.9; 8.0, 8.1; 8.2; 8.3; 8.4; 8.5, 8.6, 8.7, 8.8, 8.9; 9.0, 9.1; 9.2; 9.3; 9.4; 9.5, 9.6, 9.7, 9.8, 9.9; 10.0, 10.1; 10.2; 10.3; 10.4; 10.5, 10.6, 10.7, 10.8, 10.9; 12, 15, 16, 20, 25 or 30 compared to a reference dispersin e.g. SEQ ID NO: 1 or compared to SEQ ID NO 15.
(213) One preferred embodiment relates to a dispersin variant having improved stability, wherein HIF>1.0, compared to SEQ ID NO: 1 or compared to SEQ ID NO 15. One preferred embodiment relates to a dispersin variant having improved stability, wherein the Half-life Improvement Factor is at least 1.5, compared to SEQ ID NO: 1 or compared to SEQ ID NO 15, when measured as described in Example 2a.
(214) One preferred embodiment relates to a dispersin variant having improved stability, wherein RAR>1.0, compared to SEQ ID NO: 1 or compared to SEQ ID NO 15. One preferred embodiment relates to a dispersin variant having improved stability, wherein the residual activity ratio (RAR) is at least 1.5, compared to SEQ ID NO: 1 or compared to SEQ ID NO 15, when measured as described in Example 2a and 3.
(215) One preferred embodiment relates to a dispersin variant having improved stability, wherein HIF>1.0 compared to SEQ ID NO: 1. One preferred embodiment relates to a dispersin variant having improved stability, wherein the Half-life Improvement Factor (HIF) is at least 1.2 e.g. 1.5, compared to SEQ ID NO: 1 or compared to SEQ ID NO: 15, wherein HIF is calculated as T.sub.1/2dispersinvariant/T.sub.1/2reference and the half-life (T.sub.1/2 in minutes) for the dispersin variants and the dispersin reference (e.g. SEQ ID NO: 1 or 15) is calculated as: 20 minutes×LN(0.5)/LN(RA) and the residual activity (RA) for each dispersin variant and the reference dispersin (e.g. SEQ ID NO: 1 or 15) is calculated as: slope (stress sample, 56° C. for 60 min)/slope(unstressed sample, 21° C. for 60 min), e.g. as described in Example 2b.
(216) One preferred embodiment relates to a dispersin variant having improved stability, wherein residual activity ratio (RAR)>1.0 compared to SEQ ID NO: 1 or compared to SEQ ID NO 15. One preferred embodiment relates to a dispersin variant having improved stability, wherein the residual activity ratio (RAR) is at least 1.2 e.g. 1.5, compared to SEQ ID NO: 1 or compared to SEQ ID NO 15, wherein RAR is calculated as,
(217)
as described in 2a and 3, in the Example section.
(218) A cleaning composition as defined herein may include a dispersin variant, which compared to a dispersin with SEQ ID NO: 1, comprises an alteration e.g. substitution at a position selected from the group consisting of: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 or the polypeptide sequence shown in SEQ ID NO 15 of at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100%, wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity and wherein the variant has improved stability, measured as having an improvement factor e.g. RAR or HIF>1.0, compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO 15.
(219) A cleaning composition as defined herein may include a dispersin variant, which compared to a dispersin with SEQ ID NO: 1, comprises an alteration e.g. substitution at a position selected from the group consisting of: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 218, 221, 225, 227, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322 and 323, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 or the polypeptide sequence shown in SEQ ID NO 15 of at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100%, wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity and wherein the variant has improved stability, measured as having an improvement factor e.g. RAR or HIF>1.0, compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO 15.
(220) A cleaning composition as defined herein may include a dispersin variant, which compared to a dispersin with SEQ ID NO: 1, comprises an alteration e.g. substitution at a position selected from the group consisting of: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 or the polypeptide sequence shown in SEQ ID NO 15 of at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100%, wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity and wherein the variant has improved stability, measured as having an improvement factor e.g. RAR or HIF>1.0, compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO 15.
(221) A cleaning composition as defined herein may include a dispersin variant, which compared to a dispersin with SEQ ID NO: 1, comprises one or more alteration e.g. substitution selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124H, Y124I, Y124K, Y124L, Y124M, Y124N, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125H, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128A, V128C, V128D, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138N, D138Q, D138R, D138S, D138V, D138W, E139W, V140I, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150A, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, S163P, G164D, G164E, G164H, G164S, G164V, V167A, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181K, S181N, S181P, S181Q, S181T, S181V, S181W, E185A, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203Q, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207K, D207N, D207Q, D207R, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215K, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, S221N, G222D, E224A, E224P, S225G, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230N, D230R, D230T, D230W, E232D, E232V, N233D, N233E, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251A, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256D, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260T, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, D264N, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288P, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308E, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309E, K309G, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312E, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, Y321F and N323R, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 or to the polypeptide sequence shown in SEQ ID NO 15 of at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100%, wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity and wherein the variant has improved stability, measured as having an improvement factor e.g. RAR or HIF>1.0, compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO 15.
(222) A cleaning composition as defined herein may include a dispersin variant, which compared to a dispersin with SEQ ID NO: 1, comprises one or more alteration e.g. substitution selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181N, S181P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 or to the polypeptide sequence shown in SEQ ID NO 15 of at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100%, wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity and wherein the variant has improved stability, measured as having an improvement factor e.g. RAR or HIF>1.0, compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO 15.
(223) A cleaning composition as defined herein may include a dispersin variant, which compared to a dispersin with SEQ ID NO: 1, comprises one or more alteration e.g. substitution selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A and N323R, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 the polypeptide sequence shown in SEQ ID NO 15 of at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100%, wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity and wherein the variant has improved stability, measured as having an improvement factor e.g. RAR or HIF>1.0, compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO 15.
(224) In some preferred aspects, a cleaning composition as defined herein comprises a dispersin variant that comprises an alteration at one or more positions selected from the group consisting of 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), and wherein the variant has at least one improved property compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, preferably the improved property is improved stability, wherein stability is tested as described in example 2a, 2b or 3.
(225) In some preferred aspects, a cleaning composition as defined herein comprises a dispersin variant that comprises an alteration at one or more positions selected from the group consisting of 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 218, 221, 225, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322 and 323, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), and wherein the variant has at least one improved property compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, preferably the improved property is improved stability, wherein stability is tested as described in example 2a, 2b or 3.
(226) In a non-limiting embodiment, a dispersin variant in the compositions comprises an alteration at one or more positions selected from the group consisting of: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and wherein the variant has at least one improved property compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, preferably the improved property is improved stability, wherein stability is tested as described in example 2a, 2b or 3.
(227) In some preferred aspects, a cleaning composition as defined herein comprises a dispersin variant that comprises one or more alteration selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124H, Y124I, Y124K, Y124L, Y124M, Y124N, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125H, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128A, V128C, V128D, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138N, D138Q, D138R, D138S, D138V, D138W, E139W, V140I, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150A, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, S163P, G164D, G164E, G164H, G164S, G164V, V167A, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181K, S181N, S181P, S181Q, S181T, S181V, S181W, E185A, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203Q, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207K, D207N, D207Q, D207R, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215K, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, S221N, G222D, E224A, E224P, S225G, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230N, D230R, D230T, D230W, E232D, E232V, N233D, N233E, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251A, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256D, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260T, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, D264N, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288P, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308E, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309E, K309G, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312E, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, Y321F and N323R, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), and wherein the variant has at least one improved property compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, preferably the improved property is improved stability, wherein stability is tested as described in example 2a, 2b or 3.
(228) In some preferred aspects, a cleaning composition as defined herein comprises a dispersin variant that comprises one or more alteration selected from the group consisting of D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218K, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A and N323R, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), and wherein the variant has at least one improved property compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, preferably the improved property is improved stability, wherein stability is tested as described in example 2a, 2b or 3.
(229) In some preferred aspects, a cleaning composition as defined herein comprises a dispersin variant that comprises an alteration at one or more positions selected from the group consisting of 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each alteration provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(230) In some preferred aspects, a cleaning composition as defined herein comprises a dispersin variant that comprises an alteration at one or more positions selected from the group consisting of: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 218, 221, 225, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322 and 323, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(231) In non-limiting aspects, a dispersin variant comprises an alteration at one or more positions selected from the group consisting of: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(232) In some preferred aspects, a cleaning composition as defined herein comprises a dispersin variant that comprises one or more alteration(s) (compared to SEQ ID NO: 1), wherein the alteration is selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124H, Y124I, Y124K, Y124L, Y124M, Y124N, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125H, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128A, V128C, V128D, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138N, D138Q, D138R, D138S, D138V, D138W, E139W, V140I, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150A, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, S163P, G164D, G164E, G164H, G164S, G164V, V167A, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181K, S181N, S181P, S181Q, S181T, S181V, S181W, E185A, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203Q, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207K, D207N, D207Q, D207R, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215K, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, S221N, G222D, E224A, E224P, S225G, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230N, D230R, D230T, D230W, E232D, E232V, N233D, N233E, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251A, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256D, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260T, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, D264N, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288P, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308E, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309E, K309G, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312E, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, Y321F and N323R, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF, or residual activity, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(233) In some preferred aspects, a cleaning composition as defined herein comprises a dispersin variant that comprises one or more substitutions (compared to SEQ ID NO: 1), wherein the substitutions are selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, S225G, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A and N323R, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(234) In non-limiting aspects, the dispersin variant comprises one or more of the following substitutions: Q3F, Q3I, H15Y, T17W, A49W, N59E, V140I, S163P, S186R, D207N, Q215K, T218Q, S225G, N227T, E232D, G235W, S237W, N252P, N260Q, N267T, H272V, H272P, F276A, S279D, Y281P, S288P, K308Q, K308E, K309E, K312Q, K312E, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(235) The variants preferably comprise one or more of the conservative motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), which are shared among dispersins of the Terribacillus clade as described below. As explained in “Definitions” a clade comprises a group of polypeptides clustered together based on homologous features traced to a common ancestor. Polypeptides forming a group e.g. a clade as shown in a phylogenetic tree often share common properties and are more closely related than other polypeptides not in the clade.
(236) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] (SEQ ID NO 10), WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), and wherein the variant comprise an alteration at one or more positions selected from the list consisting of positions: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each alteration provides a dispersin variant with at least one improved property compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(237) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), and wherein the variant comprise an alteration at one or more positions selected from the list consisting of positions: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 218, 221, 225, 227, 232, 235, 237,244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322 and 323, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each alteration provides a dispersin variant with at least one improved property compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(238) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), wherein the variant comprises one or more alterations (compared to SEQ ID NO: 1), wherein the alterations are selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124H, Y124I, Y124K, Y124L, Y124M, Y124N, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125H, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128A, V128C, V128D, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138N, D138Q, D138R, D138S, D138V, D138W, E139W, V140I, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150A, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, S163P, G164D, G164E, G164H, G164S, G164V, V167A, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181K, S181N, S181P, S181Q, S181T, S181V, S181W, E185A, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203Q, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207K, D207N, D207Q, D207R, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215K, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, S221N, G222D, E224A, E224P, S225G, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230N, D230R, D230T, D230W, E232G, N233D, N233E, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251A, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256D, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260T, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, D264N, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288P, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308E, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309E, K309G, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312E, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, Y321F and N323R, wherein each substitution provides a dispersin variant with at least one improved property compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(239) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), wherein the variant comprises one or more alterations (compared to SEQ ID NO: 1), wherein the alterations are selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181N, S181P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein each substitution provides a dispersin variant with at least one improved property compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(240) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more substitution(s) (compared to SEQ ID NO: 1), wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), wherein the substitution(s) are selected from the group consisting of: D2V, Q3I, Q3F, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A and N323R, wherein each substitution provides a dispersin variant with at least one improved property compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(241) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), and wherein the variant comprise an alteration at one or more positions selected from the list consisting of positions: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each alteration provides a dispersin variant with at least one improved property compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(242) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), and wherein the variant comprise an alteration at one or more positions selected from the list consisting of positions: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(243) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), and wherein the variant comprise an alteration at one or more positions selected from the list consisting of positions: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(244) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), and wherein the variant comprise an alteration at one or more positions selected from the list consisting of positions: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 218, 221, 225, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322 and 323, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(245) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), wherein the variant comprises one or more alterations (compared to SEQ ID NO: 1), wherein the alterations are selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124H, Y124I, Y124K, Y124L, Y124M, Y124N, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125H, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128A, V128C, V128D, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138N, D138Q, D138R, D138S, D138V, D138W, E139W, V140I, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150A, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, S163P, G164D, G164E, G164H, G164S, G164V, V167A, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181K, S181N, S181P, S181Q, S181T, S181V, S181W, E185A, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203Q, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207K, D207N, D207Q, D207R, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215K, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, S221N, G222D, E224A, E224P, S225G, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230N, D230R, D230T, D230W, E232D, E232V, N233D, N233E, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251A, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256D, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260T, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, D264N, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288P, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308E, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309E, K309G, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312E, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, Y321F and N323R, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(246) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), wherein the variant comprises one or more alterations (compared to SEQ ID NO: 1), wherein the alterations are selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181N, S181P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(247) A cleaning composition as defined herein may include a variant of a dispersin parent, wherein the variant comprises one or more motifs GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), wherein the variant comprises one or more alterations (compared to SEQ ID NO: 1), wherein the alterations are selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A and N323R, wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(248) Addition of any one of the mutations, improves stability.
(249) In some preferred aspects, a dispersin variant used herein comprises 2, 3, 4, 5, 6, 7, 8 or 9 or more substitutions (compared to SEQ ID NO: 1), wherein the substitutions are selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, S225G, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A and N323R, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and wherein the variant has at least one improved property compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, preferably the improved property is, improved stability, wherein stability is tested as described in Example 4 and Example 5 and preferably wherein the variant has hexosaminidase e.g. beta-1,6 N-acetylglucosaminidase activity.
(250) In some aspects, the dispersin variant has an improved stability, measured as Half-life Improvement Factor, HIF, compared to the parent or compared to the dispersin having the polypeptide shown in SEQ ID NO: 1 or compared to a dispersin having the identical amino acid sequence of the dispersin variant but not having the alterations at 2, 3, 4, 5, 6, 7, 8 or 9 or more of the specified positions.
(251) In some aspects, the dispersin variant has an improved stability, measured as residual activity ratio, RAR, compared to the parent or compared to the dispersin having the polypeptide shown in SEQ ID NO: 1 or compared to a dispersin having the identical amino acid sequence of the dispersin variant but not having the alterations at 2, 3, 4, 5, 6, 7, 8 or 9 or more of the specified positions.
(252) In one embodiment the dispersin variant comprised in the inventive cleaning compositions comprises one or more of the following substitution(s), Q3F, Q3I, H15Y, T17W, A49W, N59E, V140I, S163P, S186R, D207N, Q215K, T218Q, S225G, N227T, E232D, G235W, S237W, N252P, N260Q, N267T, H272V, H272P, S279D, Y281P, S288P, K308Q, K309E, K312Q, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(253) In one embodiment the dispersin variant comprised in the cleaning compositions comprises one or more of the following substitution(s), Q3I, H15Y, A49W, N59E, S163P, S186R, S225G, N227T, E232D, G235W, N252P, N260Q, H272V, S279D, Y281P, K308Q, K309E, K312Q, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(254) In a non-limiting embodiment the dispersin variant comprised in the cleaning compositions comprises one or more of the following substitution sets: Q3I+A49W, Q3I+N59E, Q3I+S163P, Q3I+S186R, Q3I+Q215K, Q3I+S225G, Q3I+N227T, Q3I+N252P, Q3I+N267T, Q3I+F276A, Q3I+Y281P, Q3I+K308Q, Q3I+K308E, Q3I+K309E, Q3I+K312Q, Q3I+K312E, Q3F+A49W, Q3F+N59E, Q3F+S163P, Q3F+S186R, Q3F+Q215K, Q3F+S225G, Q3F+N227T, Q3F+N252P, Q3F+N267T, Q3F+F276A, Q3F+Y281P, Q3F+K308Q, Q3F+K308E, Q3F+K309E, Q3F+K312E, Q3F+K312Q, A49W+N59E, A49W+S163P, A49W+S186R, A49W+Q215K, A49W+S225G, A49W+N227T, A49W+N252P, A49W+N267T, A49W+F276A, A49W+Y281P, A49W+K308Q, A49W+K308E, A49W+K309E, A49W+K312Q, A49W+K312E, N59E+S163P, N59E+S186R, N59E+Q215K, N59E+S225G, N59E+N227T, N59E+N252P, N59E+N267T, N59E+F276A, N59E+Y281P, N59E+K308Q, N59E+K308E, N59E+K309E, N59E+K312Q, N59E+K312E, S163P+S186R, S163P+Q215K, S163P+S225G, S163P+N227T, S163P+N252P, S163P+N267T, S163P+F276A, S163P+Y281P, S163P+K308E, S163P+K308Q, S163P+K309E, S163P+K312Q, S163P+K312E, S186R+Q215K, S186R+S225G, S186R+N227T, S186R+N252P, S186R+N267T, S186R+F276A, S186R+Y281P, S186R+K308E, S186R+K308Q, S186R+K309E, S186R+K312Q, S186R+K312E, Q215K+S225G, Q215K+N227T, Q215K+N252P, Q215K+N267T, Q215K+F276A, Q215K+Y281P, Q215K+K308E, Q215K+K308Q, Q215K+K309E, Q215K+K312Q Q215K+K312E, S225G+N227T, S225G+N252P, S225G+N267T, S225G+F276A, S225G+Y281P, S225G+K308E, S225G+K308Q, S225G+K309E, S225G+K312Q, S225G+K312E, N227T+N252P, N227T+N267T, N227T+F276A, N227T+Y281P, N227T+K308Q, N227T+K308E, N227T+K309E, N227T+K312Q N227T+K312E, N252P+N267T, N252P+F276A, N252P+Y281P, N252P+K308Q, N252P+K308E, N252P+K309E, N252P+K312Q, N252P+K312E, N267T+F276A, N267T+Y281P, N267T+K308E, N267T+K309E, N267T+K312Q, N267T+K312E, F276A+Y281P, F276A+K308Q, F276A+K308E, F276A+K309E, F276A+K312Q, F276A+K312E, Y281P+K308Q, Y281P+K308E, Y281P+K309E, Y281P+K312Q, Y281P+K312E, K308E+K312Q, K308E+K312E, K308Q+K312Q, K309E+K312Q or K309E+K312E, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(255) In one embodiment the dispersin variant comprised in the cleaning compositions comprises at least two substitutions wherein the substitutions or substitution sets are selected from the group consisting of: F276A+K308E, F276A+K309E, K308E+K309E, K308E+K312E, K309E+K312E, N227T+F276A, N227T+K308E, N227T+K312E, N227T+N252P, N227T+N267T, N252P+F276A, N252P+K308E, N252P+K309E, N252P+N267T, N267T+F276A, N267T+K308E, N267T+K309E, N267T+K312E, Q215K+F276A, Q215K+K308E, Q215K+K309E, Q215K+K312E, Q215K+N227T, Q215K+N252P, Q215K+N267T, S163P+F276A, S163P+K308E, S163P+K309E, S163P+K312E, S163P+N252P, S163P+N267T and S163P+Q215K.
(256) In one embodiment the dispersin variant comprised in the cleaning compositions comprises at least two substitutions, wherein the substitution sets are selected from the group consisting of: Q3I+H15Y, Q3I+A49W, Q3I+N59E, Q3I+S163P, Q3I+S186R, Q3I+S225G, Q3I+N227T, Q3I+E232D, Q3I+G235W, Q3I+N252P, Q3I+N260Q, Q3I+H272V, Q3I+S279D, Q3I+Y281P, Q3I+K308Q, Q3I+K309E, Q3I+K312Q, H15Y+A49W, H15Y+N59E, H15Y+S163P, H15Y+S186R, H15Y+S225G, H15Y+N227T, H15Y+E232D, H15Y+G235W, H15Y+N252P, H15Y+N260Q, H15Y+H272V, H15Y+S279D, H15Y+Y281P, H15Y+K308Q, H15Y+K309E, H15Y+K312Q, A49W+N59E, A49W+S163P, A49W+S186R, A49W+S225G, A49W+N227T, A49W+E232D, A49W+G235W, A49W+N252P, A49W+N260Q, A49W+H272V, A49W+S279D, A49W+Y281P, A49W+K308Q, A49W+K309E, A49W+K312Q, N59E+S163P, N59E+S186R, N59E+S225G, N59E+N227T, N59E+E232D, N59E+G235W, N59E+N252P, N59E+N260Q, N59E+H272V, N59E+S279D, N59E+Y281P, N59E+K308Q, N59E+K309E, N59E+K312Q, S163P+S186R, S163P+S225G, S163P+N227T, S163P+E232D, S163P+G235W, S163P+N252P, S163P+N260Q, S163P+H272V, S163P+S279D, S163P+Y281P, S163P+K308Q, S163P+K309E, S163P+K312Q, S186R+S225G, S186R+N227T, S186R+E232D, S186R+G235W, S186R+N252P, S186R+N260Q, S186R+H272V, S186R+S279D, S186R+Y281P, S186R+K308Q, S186R+K309E, S186R+K312Q, S225G+N227T, S225G+E232D, S225G+G235W, S225G+N252P, S225G+N260Q, S225G+H272V, S225G+S279D, S225G+Y281P, S225G+K308Q, S225G+K309E, S225G+K312Q, N227T+E232D, N227T+G235W, N227T+N252P, N227T+N260Q, N227T+H272V, N227T+S279D, N227T+Y281P, N227T+K308Q, N227T+K309E, N227T+K312Q, E232D+G235W, E232D+N252P, E232D+N260Q, E232D+H272V, E232D+S279D, E232D+Y281P, E232D+K308Q, E232D+K309E, E232D+K312Q, G235W+N252P, G235W+N260Q, G235W+H272V, G235W+S279D, G235W+Y281P, G235W+K308Q, G235W+K309E, G235W+K312Q, N252P+N260Q, N252P+H272V, N252P+S279D, N252P+Y281P, N252P+K308Q, N252P+K309E, N252P+K312Q, N260Q+H272V, N260Q+S279D, N260Q+Y281P, N260Q+K308Q, N260Q+K309E, N260Q+K312Q, H272V+S279D, H272V+Y281P, H272V+K308Q, H272V+K309E, H272V+K312Q, S279D+Y281P, S279D+K308Q, S279D+K309E, S279D+K312Q, Y281P+K308Q, Y281P+K309E, Y281P+K312Q, K308Q+K309E, K308Q+K312Q and K309E+K312Q.
(257) In one embodiment, the dispersin variant comprises at least three substitutions, wherein the substitutions or substitution sets are selected from the group consisting of:
(258) K312E+N227T+F276A, K312E+N227T+K308E, K312E+N227T+K309E, K312E+N227T+K312E,
(259) K312E+F276A+K308E, K312E+F276A+K309E, K312E+F276A+K312E, K312E+K308E+K309E,
(260) K312E+K308E+K312E, K312E+K309E+K312E, N227T+F276A+K308E, N227T+F276A+K309E,
(261) N227T+F276A+K312E, N227T+K308E+K309E, N227T+K308E+K312E, N227T+K309E+K312E,
(262) F276A+K308E+K309E, F276A+K308E+K312E, F276A+K309E+K312E, and K308E+K309E+K312E.
(263) In one embodiment, the dispersin variant comprises at least three substitutions, wherein the substitution sets are selected from the group consisting of:
(264) Q3I+H15Y+A49W, Q3I+H15Y+N59E, Q3I+H15Y+S163P, Q3I+H15Y+S186R, Q3I+H15Y+S225G,
(265) Q3I+H15Y+N227T, Q3I+H15Y+E232D, Q3I+H15Y+G235W, Q3I+H15Y+N252P, Q3I+H15Y+N260Q,
(266) Q3I+H15Y+H272V, Q3I+H15Y+S279D, Q3I+H15Y+Y281P, Q3I+H15Y+K308Q, Q3I+H15Y+K309E,
(267) Q3I+H15Y+K312Q, Q3I+A49W+N59E, Q3I+A49W+S163P, Q3I+A49W+S186R, Q3I+A49W+S225G,
(268) Q3I+A49W+N227T, Q3I+A49W+E232D, Q3I+A49W+G235W, Q3I+A49W+N252P, Q3I+A49W+N260Q,
(269) Q3I+A49W+H272V, Q3I+A49W+S279D, Q3I+A49W+Y281P, Q3I+A49W+K308Q, Q3I+A49W+K309E,
(270) Q3I+A49W+K312Q, Q3I+N59E+S163P, Q3I+N59E+S186R, Q3I+N59E+S225G, Q3I+N59E+N227T,
(271) Q3I+N59E+E232D, Q3I+N59E+G235W, Q3I+N59E+N252P, Q3I+N59E+N260Q, Q3I+N59E+H272V,
(272) Q3I+N59E+S279D, Q3I+N59E+Y281P, Q3I+N59E+K308Q, Q3I+N59E+K309E, Q3I+N59E+K312Q,
(273) Q3I+S163P+S186R, Q3I+S163P+S225G, Q3I+S163P+N227T, Q3I+S163P+E232D, Q3I+S163P+G235W,
(274) Q3I+S163P+N252P, Q3I+S163P+N260Q, Q3I+S163P+H272V, Q3I+S163P+S279D, Q3I+S163P+Y281P,
(275) Q3I+S163P+K308Q, Q3I+S163P+K309E, Q3I+S163P+K312Q, Q3I+S186R+S225G, Q3I+S186R+N227T,
(276) Q3I+S186R+E232D, Q3I+S186R+G235W, Q3I+S186R+N252P, Q3I+S186R+N260Q,
(277) Q3I+S186R+H272V, Q3I+S186R+S279D, Q3I+S186R+Y281P, Q3I+S186R+K308Q, Q3I+S186R+K309E,
(278) Q3I+S186R+K312Q, Q3I+S225G+N227T, Q3I+S225G+E232D, Q3I+S225G+G235W,
(279) Q3I+S225G+N252P, Q3I+S225G+N260Q, Q3I+S225G+H272V, Q3I+S225G+S279D,
(280) Q3I+S225G+Y281P, Q3I+S225G+K308Q, Q3I+S225G+K309E, Q3I+S225G+K312Q, Q3I+N227T+E232D,
(281) Q3I+N227T+G235W, Q3I+N227T+N252P, Q3I+N227T+N260Q, Q3I+N227T+H272V, Q3I+N227T+S279D,
(282) Q3I+N227T+Y281P, Q3I+N227T+K308Q, Q3I+N227T+K309E, Q3I+N227T+K312Q, Q3I+E232D+G235W,
(283) Q3I+E232D+N252P, Q3I+E232D+N260Q, Q3I+E232D+H272V, Q3I+E232D+S279D, Q3I+E232D+Y281P,
(284) Q3I+E232D+K308Q, Q3I+E232D+K309E, Q3I+E232D+K312Q, Q3I+G235W+N252P,
(285) Q3I+G235W+N260Q, Q3I+G235W+H272V, Q3I+G235W+S279D, Q3I+G235W+Y281P,
(286) Q3I+G235W+K308Q, Q3I+G235W+K309E, Q3I+G235W+K312Q, Q3I+N252P+N260Q,
(287) Q3I+N252P+H272V, Q3I+N252P+S279D, Q3I+N252P+Y281P, Q3I+N252P+K308Q, Q3I+N252P+K309E,
(288) Q3I+N252P+K312Q, Q3I+N260Q+H272V, Q3I+N260Q+S279D, Q3I+N260Q+Y281P,
(289) Q3I+N260Q+K308Q, Q3I+N260Q+K309E, Q3I+N260Q+K312Q, Q3I+H272V+S279D,
(290) Q3I+H272V+Y281P, Q3I+H272V+K308Q, Q3I+H272V+K309E, Q3I+H272V+K312Q, Q3I+S279D+Y281P,
(291) Q3I+S279D+K308Q, Q3I+S279D+K309E, Q3I+S279D+K312Q, Q3I+Y281P+K308Q, Q3I+Y281P+K309E,
(292) Q3I+Y281P+K312Q, Q3I+K308Q+K309E, Q3I+K308Q+K312Q, Q3I+K309E+K312Q, H15Y+A49W+N59E,
(293) H15Y+A49W+S163P, H15Y+A49W+S186R, H15Y+A49W+S225G, H15Y+A49W+N227T,
(294) H15Y+A49W+E232D, H15Y+A49W+G235W, H15Y+A49W+N252P, H15Y+A49W+N260Q,
(295) H15Y+A49W+H272V, H15Y+A49W+S279D, H15Y+A49W+Y281P, H15Y+A49W+K308Q,
(296) H15Y+A49W+K309E, H15Y+A49W+K312Q, H15Y+N59E+S163P, H15Y+N59E+S186R,
(297) H15Y+N59E+S225G, H15Y+N59E+N227T, H15Y+N59E+E232D, H15Y+N59E+G235W,
(298) H15Y+N59E+N252P, H15Y+N59E+N260Q, H15Y+N59E+H272V, H15Y+N59E+S279D,
(299) H15Y+N59E+Y281P, H15Y+N59E+K308Q, H15Y+N59E+K309E, H15Y+N59E+K312Q,
(300) H15Y+S163P+S186R, H15Y+S163P+S225G, H15Y+S163P+N227T, H15Y+S163P+E232D,
(301) H15Y+S163P+G235W, H15Y+S163P+N252P, H15Y+S163P+N260Q, H15Y+S163P+H272V,
(302) H15Y+S163P+S279D, H15Y+S163P+Y281P, H15Y+S163P+K308Q, H15Y+S163P+K309E,
(303) H15Y+S163P+K312Q, H15Y+S186R+S225G, H15Y+S186R+N227T, H15Y+S186R+E232D,
(304) H15Y+S186R+G235W, H15Y+S186R+N252P, H15Y+S186R+N260Q, H15Y+S186R+H272V,
(305) H15Y+S186R+S279D, H15Y+S186R+Y281P, H15Y+S186R+K308Q, H15Y+S186R+K309E,
(306) H15Y+S186R+K312Q, H15Y+S225G+N227T, H15Y+S225G+E232D, H15Y+S225G+G235W,
(307) H15Y+S225G+N252P, H15Y+S225G+N260Q, H15Y+S225G+H272V, H15Y+S225G+S279D,
(308) H15Y+S225G+Y281P, H15Y+S225G+K308Q, H15Y+S225G+K309E, H15Y+S225G+K312Q,
(309) H15Y+N227T+E232D, H15Y+N227T+G235W, H15Y+N227T+N252P, H15Y+N227T+N260Q,
(310) H15Y+N227T+H272V, H15Y+N227T+S279D, H15Y+N227T+Y281P, H15Y+N227T+K308Q,
(311) H15Y+N227T+K309E, H15Y+N227T+K312Q, H15Y+E232D+G235W, H15Y+E232D+N252P,
(312) H15Y+E232D+N260Q, H15Y+E232D+H272V, H15Y+E232D+S279D, H15Y+E232D+Y281P,
(313) H15Y+E232D+K308Q, H15Y+E232D+K309E, H15Y+E232D+K312Q, H15Y+G235W+N252P,
(314) H15Y+G235W+N260Q, H15Y+G235W+H272V, H15Y+G235W+S279D, H15Y+G235W+Y281P,
(315) H15Y+G235W+K308Q, H15Y+G235W+K309E, H15Y+G235W+K312Q, H15Y+N252P+N260Q,
(316) H15Y+N252P+H272V, H15Y+N252P+S279D, H15Y+N252P+Y281P, H15Y+N252P+K308Q,
(317) H15Y+N252P+K309E, H15Y+N252P+K312Q, H15Y+N260Q+H272V, H15Y+N260Q+S279D,
(318) H15Y+N260Q+Y281P, H15Y+N260Q+K308Q, H15Y+N260Q+K309E, H15Y+N260Q+K312Q,
(319) H15Y+H272V+S279D, H15Y+H272V+Y281P, H15Y+H272V+K308Q, H15Y+H272V+K309E,
(320) H15Y+H272V+K312Q, H15Y+S279D+Y281P, H15Y+S279D+K308Q, H15Y+S279D+K309E,
(321) H15Y+S279D+K312Q, H15Y+Y281P+K308Q, H15Y+Y281P+K309E, H15Y+Y281P+K312Q,
(322) H15Y+K308Q+K309E, H15Y+K308Q+K312Q, H15Y+K309E+K312Q, A49W+N59E+S163P,
(323) A49W+N59E+S186R, A49W+N59E+S225G, A49W+N59E+N227T, A49W+N59E+E232D,
(324) A49W+N59E+G235W, A49W+N59E+N252P, A49W+N59E+N260Q, A49W+N59E+H272V,
(325) A49W+N59E+S279D, A49W+N59E+Y281P, A49W+N59E+K308Q, A49W+N59E+K309E,
(326) A49W+N59E+K312Q, A49W+S163P+S186R, A49W+S163P+S225G, A49W+S163P+N227T,
(327) A49W+S163P+E232D, A49W+S163P+G235W, A49W+S163P+N252P, A49W+S163P+N260Q,
(328) A49W+S163P+H272V, A49W+S163P+S279D, A49W+S163P+Y281P, A49W+S163P+K308Q,
(329) A49W+S163P+K309E, A49W+S163P+K312Q, A49W+S186R+S225G, A49W+S186R+N227T,
(330) A49W+S186R+E232D, A49W+S186R+G235W, A49W+S186R+N252P, A49W+S186R+N260Q,
(331) A49W+S186R+H272V, A49W+S186R+S279D, A49W+S186R+Y281P, A49W+S186R+K308Q,
(332) A49W+S186R+K309E, A49W+S186R+K312Q, A49W+S225G+N227T, A49W+S225G+E232D,
(333) A49W+S225G+G235W, A49W+S225G+N252P, A49W+S225G+N260Q, A49W+S225G+H272V,
(334) A49W+S225G+S279D, A49W+S225G+Y281P, A49W+S225G+K308Q, A49W+S225G+K309E,
(335) A49W+S225G+K312Q, A49W+N227T+E232D, A49W+N227T+G235W, A49W+N227T+N252P,
(336) A49W+N227T+N260Q, A49W+N227T+H272V, A49W+N227T+S279D, A49W+N227T+Y281P,
(337) A49W+N227T+K308Q, A49W+N227T+K309E, A49W+N227T+K312Q, A49W+E232D+G235W,
(338) A49W+E232D+N252P, A49W+E232D+N260Q, A49W+E232D+H272V, A49W+E232D+S279D,
(339) A49W+E232D+Y281P, A49W+E232D+K308Q, A49W+E232D+K309E, A49W+E232D+K312Q,
(340) A49W+G235W+N252P, A49W+G235W+N260Q, A49W+G235W+H272V, A49W+G235W+S279D,
(341) A49W+G235W+Y281P, A49W+G235W+K308Q, A49W+G235W+K309E, A49W+G235W+K312Q,
(342) A49W+N252P+N260Q, A49W+N252P+H272V, A49W+N252P+S279D, A49W+N252P+Y281P,
(343) A49W+N252P+K308Q, A49W+N252P+K309E, A49W+N252P+K312Q, A49W+N260Q+H272V,
(344) A49W+N260Q+S279D, A49W+N260Q+Y281P, A49W+N260Q+K308Q, A49W+N260Q+K309E,
(345) A49W+N260Q+K312Q, A49W+H272V+S279D, A49W+H272V+Y281P, A49W+H272V+K308Q,
(346) A49W+H272V+K309E, A49W+H272V+K312Q, A49W+S279D+Y281P, A49W+S279D+K308Q,
(347) A49W+S279D+K309E, A49W+S279D+K312Q, A49W+Y281P+K308Q, A49W+Y281P+K309E,
(348) A49W+Y281P+K312Q, A49W+K308Q+K309E, A49W+K308Q+K312Q, A49W+K309E+K312Q,
(349) N59E+S163P+S186R, N59E+S163P+S225G, N59E+S163P+N227T, N59E+S163P+E232D,
(350) N59E+S163P+G235W, N59E+S163P+N252P, N59E+S163P+N260Q, N59E+S163P+H272V,
(351) N59E+S163P+S279D, N59E+S163P+Y281P, N59E+S163P+K308Q, N59E+S163P+K309E,
(352) N59E+S163P+K312Q, N59E+S186R+S225G, N59E+S186R+N227T, N59E+S186R+E232D,
(353) N59E+S186R+G235W, N59E+S186R+N252P, N59E+S186R+N260Q, N59E+S186R+H272V,
(354) N59E+S186R+S279D, N59E+S186R+Y281P, N59E+S186R+K308Q, N59E+S186R+K309E,
(355) N59E+S186R+K312Q, N59E+S225G+N227T, N59E+S225G+E232D, N59E+S225G+G235W,
(356) N59E+S225G+N252P, N59E+S225G+N260Q, N59E+S225G+H272V, N59E+S225G+S279D,
(357) N59E+S225G+Y281P, N59E+S225G+K308Q, N59E+S225G+K309E, N59E+S225G+K312Q,
(358) N59E+N227T+E232D, N59E+N227T+G235W, N59E+N227T+N252P, N59E+N227T+N260Q,
(359) N59E+N227T+H272V, N59E+N227T+S279D, N59E+N227T+Y281P, N59E+N227T+K308Q,
(360) N59E+N227T+K309E, N59E+N227T+K312Q, N59E+E232D+G235W, N59E+E232D+N252P,
(361) N59E+E232D+N260Q, N59E+E232D+H272V, N59E+E232D+S279D, N59E+E232D+Y281P,
(362) N59E+E232D+K308Q, N59E+E232D+K309E, N59E+E232D+K312Q, N59E+G235W+N252P,
(363) N59E+G235W+N260Q, N59E+G235W+H272V, N59E+G235W+S279D, N59E+G235W+Y281P,
(364) N59E+G235W+K308Q, N59E+G235W+K309E, N59E+G235W+K312Q, N59E+N252P+N260Q,
(365) N59E+N252P+H272V, N59E+N252P+S279D, N59E+N252P+Y281P, N59E+N252P+K308Q,
(366) N59E+N252P+K309E, N59E+N252P+K312Q, N59E+N260Q+H272V, N59E+N260Q+S279D,
(367) N59E+N260Q+Y281P, N59E+N260Q+K308Q, N59E+N260Q+K309E, N59E+N260Q+K312Q,
(368) N59E+H272V+S279D, N59E+H272V+Y281P, N59E+H272V+K308Q, N59E+H272V+K309E,
(369) N59E+H272V+K312Q, N59E+S279D+Y281P, N59E+S279D+K308Q, N59E+S279D+K309E,
(370) N59E+S279D+K312Q, N59E+Y281P+K308Q, N59E+Y281P+K309E, N59E+Y281P+K312Q,
(371) N59E+K308Q+K309E, N59E+K308Q+K312Q, N59E+K309E+K312Q, S163P+S186R+S225G,
(372) S163P+S186R+N227T, S163P+S186R+E232D, S163P+S186R+G235W, S163P+S186R+N252P,
(373) S163P+S186R+N260Q, S163P+S186R+H272V, S163P+S186R+S279D, S163P+S186R+Y281P,
(374) S163P+S186R+K308Q, S163P+S186R+K309E, S163P+S186R+K312Q, S163P+S225G+N227T,
(375) S163P+S225G+E232D, S163P+S225G+G235W, S163P+S225G+N252P, S163P+S225G+N260Q,
(376) S163P+S225G+H272V, S163P+S225G+S279D, S163P+S225G+Y281P, S163P+S225G+K308Q,
(377) S163P+S225G+K309E, S163P+S225G+K312Q, S163P+N227T+E232D, S163P+N227T+G235W,
(378) S163P+N227T+N252P, S163P+N227T+N260Q, S163P+N227T+H272V, S163P+N227T+S279D,
(379) S163P+N227T+Y281P, S163P+N227T+K308Q, S163P+N227T+K309E, S163P+N227T+K312Q,
(380) S163P+E232D+G235W, S163P+E232D+N252P, S163P+E232D+N260Q, S163P+E232D+H272V,
(381) S163P+E232D+S279D, S163P+E232D+Y281P, S163P+E232D+K308Q, S163P+E232D+K309E,
(382) S163P+E232D+K312Q, S163P+G235W+N252P, S163P+G235W+N260Q, S163P+G235W+H272V,
(383) S163P+G235W+S279D, S163P+G235W+Y281P, S163P+G235W+K308Q, S163P+G235W+K309E,
(384) S163P+G235W+K312Q, S163P+N252P+N260Q, S163P+N252P+H272V, S163P+N252P+S279D,
(385) S163P+N252P+Y281P, S163P+N252P+K308Q, S163P+N252P+K309E, S163P+N252P+K312Q,
(386) S163P+N260Q+H272V, S163P+N260Q+S279D, S163P+N260Q+Y281P, S163P+N260Q+K308Q,
(387) S163P+N260Q+K309E, S163P+N260Q+K312Q, S163P+H272V+S279D, S163P+H272V+Y281P,
(388) S163P+H272V+K308Q, S163P+H272V+K309E, S163P+H272V+K312Q, S163P+S279D+Y281P,
(389) S163P+S279D+K308Q, S163P+S279D+K309E, S163P+S279D+K312Q, S163P+Y281P+K308Q,
(390) S163P+Y281P+K309E, S163P+Y281P+K312Q, S163P+K308Q+K309E, S163P+K308Q+K312Q,
(391) S163P+K309E+K312Q, S186R+S225G+N227T, S186R+S225G+E232D, S186R+S225G+G235W,
(392) S186R+S225G+N252P, S186R+S225G+N260Q, S186R+S225G+H272V, S186R+S225G+S279D,
(393) S186R+S225G+Y281P, S186R+S225G+K308Q, S186R+S225G+K309E, S186R+S225G+K312Q,
(394) S186R+N227T+E232D, S186R+N227T+G235W, S186R+N227T+N252P, S186R+N227T+N260Q,
(395) S186R+N227T+H272V, S186R+N227T+S279D, S186R+N227T+Y281P, S186R+N227T+K308Q,
(396) S186R+N227T+K309E, S186R+N227T+K312Q, S186R+E232D+G235W, S186R+E232D+N252P,
(397) S186R+E232D+N260Q, S186R+E232D+H272V, S186R+E232D+S279D, S186R+E232D+Y281P,
(398) S186R+E232D+K308Q, S186R+E232D+K309E, S186R+E232D+K312Q, S186R+G235W+N252P,
(399) S186R+G235W+N260Q, S186R+G235W+H272V, S186R+G235W+S279D, S186R+G235W+Y281P,
(400) S186R+G235W+K308Q, S186R+G235W+K309E, S186R+G235W+K312Q, S186R+N252P+N260Q,
(401) S186R+N252P+H272V, S186R+N252P+S279D, S186R+N252P+Y281P, S186R+N252P+K308Q,
(402) S186R+N252P+K309E, S186R+N252P+K312Q, S186R+N260Q+H272V, S186R+N260Q+S279D,
(403) S186R+N260Q+Y281P, S186R+N260Q+K308Q, S186R+N260Q+K309E, S186R+N260Q+K312Q,
(404) S186R+H272V+S279D, S186R+H272V+Y281P, S186R+H272V+K308Q, S186R+H272V+K309E,
(405) S186R+H272V+K312Q, S186R+S279D+Y281P, S186R+S279D+K308Q, S186R+S279D+K309E,
(406) S186R+S279D+K312Q, S186R+Y281P+K308Q, S186R+Y281P+K309E, S186R+Y281P+K312Q,
(407) S186R+K308Q+K309E, S186R+K308Q+K312Q, S186R+K309E+K312Q, S225G+N227T+E232D,
(408) S225G+N227T+G235W, S225G+N227T+N252P, S225G+N227T+N260Q, S225G+N227T+H272V,
(409) S225G+N227T+S279D, S225G+N227T+Y281P, S225G+N227T+K308Q, S225G+N227T+K309E,
(410) S225G+N227T+K312Q, S225G+E232D+G235W, S225G+E232D+N252P, S225G+E232D+N260Q,
(411) S225G+E232D+H272V, S225G+E232D+S279D, S225G+E232D+Y281P, S225G+E232D+K308Q,
(412) S225G+E232D+K309E, S225G+E232D+K312Q, S225G+G235W+N252P, S225G+G235W+N260Q,
(413) S225G+G235W+H272V, S225G+G235W+S279D, S225G+G235W+Y281P, S225G+G235W+K308Q,
(414) S225G+G235W+K309E, S225G+G235W+K312Q, S225G+N252P+N260Q, S225G+N252P+H272V,
(415) S225G+N252P+S279D, S225G+N252P+Y281P, S225G+N252P+K308Q, S225G+N252P+K309E,
(416) S225G+N252P+K312Q, S225G+N260Q+H272V, S225G+N260Q+S279D, S225G+N260Q+Y281P,
(417) S225G+N260Q+K308Q, S225G+N260Q+K309E, S225G+N260Q+K312Q, S225G+H272V+S279D,
(418) S225G+H272V+Y281P, S225G+H272V+K308Q, S225G+H272V+K309E, S225G+H272V+K312Q,
(419) S225G+S279D+Y281P, S225G+S279D+K308Q, S225G+S279D+K309E, S225G+S279D+K312Q,
(420) S225G+Y281P+K308Q, S225G+Y281P+K309E, S225G+Y281P+K312Q, S225G+K308Q+K309E,
(421) S225G+K308Q+K312Q, S225G+K309E+K312Q, N227T+E232D+G235W, N227T+E232D+N252P,
(422) N227T+E232D+N260Q, N227T+E232D+H272V, N227T+E232D+S279D, N227T+E232D+Y281P,
(423) N227T+E232D+K308Q, N227T+E232D+K309E, N227T+E232D+K312Q, N227T+G235W+N252P,
(424) N227T+G235W+N260Q, N227T+G235W+H272V, N227T+G235W+S279D, N227T+G235W+Y281P,
(425) N227T+G235W+K308Q, N227T+G235W+K309E, N227T+G235W+K312Q, N227T+N252P+N260Q,
(426) N227T+N252P+H272V, N227T+N252P+S279D, N227T+N252P+Y281P, N227T+N252P+K308Q,
(427) N227T+N252P+K309E, N227T+N252P+K312Q, N227T+N260Q+H272V, N227T+N260Q+S279D,
(428) N227T+N260Q+Y281P, N227T+N260Q+K308Q, N227T+N260Q+K309E, N227T+N260Q+K312Q,
(429) N227T+H272V+S279D, N227T+H272V+Y281P, N227T+H272V+K308Q, N227T+H272V+K309E,
(430) N227T+H272V+K312Q, N227T+S279D+Y281P, N227T+S279D+K308Q, N227T+S279D+K309E,
(431) N227T+S279D+K312Q, N227T+Y281P+K308Q, N227T+Y281P+K309E, N227T+Y281P+K312Q,
(432) N227T+K308Q+K309E, N227T+K308Q+K312Q, N227T+K309E+K312Q, E232D+G235W+N252P,
(433) E232D+G235W+N260Q, E232D+G235W+H272V, E232D+G235W+S279D, E232D+G235W+Y281P,
(434) E232D+G235W+K308Q, E232D+G235W+K309E, E232D+G235W+K312Q, E232D+N252P+N260Q,
(435) E232D+N252P+H272V, E232D+N252P+S279D, E232D+N252P+Y281P, E232D+N252P+K308Q,
(436) E232D+N252P+K309E, E232D+N252P+K312Q, E232D+N260Q+H272V, E232D+N260Q+S279D,
(437) E232D+N260Q+Y281P, E232D+N260Q+K308Q, E232D+N260Q+K309E, E232D+N260Q+K312Q,
(438) E232D+H272V+S279D, E232D+H272V+Y281P, E232D+H272V+K308Q, E232D+H272V+K309E,
(439) E232D+H272V+K312Q, E232D+S279D+Y281P, E232D+S279D+K308Q, E232D+S279D+K309E,
(440) E232D+S279D+K312Q, E232D+Y281P+K308Q, E232D+Y281P+K309E, E232D+Y281P+K312Q,
(441) E232D+K308Q+K309E, E232D+K308Q+K312Q, E232D+K309E+K312Q, G235W+N252P+N260Q,
(442) G235W+N252P+H272V, G235W+N252P+S279D, G235W+N252P+Y281P, G235W+N252P+K308Q,
(443) G235W+N252P+K309E, G235W+N252P+K312Q, G235W+N260Q+H272V, G235W+N260Q+S279D,
(444) G235W+N260Q+Y281P, G235W+N260Q+K308Q, G235W+N260Q+K309E, G235W+N260Q+K312Q,
(445) G235W+H272V+S279D, G235W+H272V+Y281P, G235W+H272V+K308Q, G235W+H272V+K309E,
(446) G235W+H272V+K312Q, G235W+S279D+Y281P, G235W+S279D+K308Q, G235W+S279D+K309E,
(447) G235W+S279D+K312Q, G235W+Y281P+K308Q, G235W+Y281P+K309E, G235W+Y281P+K312Q,
(448) G235W+K308Q+K309E, G235W+K308Q+K312Q, G235W+K309E+K312Q, N252P+N260Q+H272V,
(449) N252P+N260Q+S279D, N252P+N260Q+Y281P, N252P+N260Q+K308Q, N252P+N260Q+K309E,
(450) N252P+N260Q+K312Q, N252P+H272V+S279D, N252P+H272V+Y281P, N252P+H272V+K308Q,
(451) N252P+H272V+K309E, N252P+H272V+K312Q, N252P+S279D+Y281P, N252P+S279D+K308Q,
(452) N252P+S279D+K309E, N252P+S279D+K312Q, N252P+Y281P+K308Q, N252P+Y281P+K309E,
(453) N252P+Y281P+K312Q, N252P+K308Q+K309E, N252P+K308Q+K312Q, N252P+K309E+K312Q,
(454) N260Q+H272V+S279D, N260Q+H272V+Y281P, N260Q+H272V+K308Q, N260Q+H272V+K309E,
(455) N260Q+H272V+K312Q, N260Q+S279D+Y281P, N260Q+S279D+K308Q, N260Q+S279D+K309E,
(456) N260Q+S279D+K312Q, N260Q+Y281P+K308Q, N260Q+Y281P+K309E, N260Q+Y281P+K312Q,
(457) N260Q+K308Q+K309E, N260Q+K308Q+K312Q, N260Q+K309E+K312Q, H272V+S279D+Y281P,
(458) H272V+S279D+K308Q, H272V+S279D+K309E, H272V+S279D+K312Q, H272V+Y281P+K308Q,
(459) H272V+Y281P+K309E, H272V+Y281P+K312Q, H272V+K308Q+K309E, H272V+K308Q+K312Q,
(460) H272V+K309E+K312Q, S279D+Y281P+K308Q, S279D+Y281P+K309E, S279D+Y281P+K312Q,
(461) S279D+K308Q+K309E, S279D+K308Q+K312Q, S279D+K309E+K312Q, Y281P+K308Q+K309E,
(462) Y281P+K308Q+K312Q, Y281P+K309E+K312Q and K308Q+K309E+K312Q.
(463) In one embodiment, the dispersin variant comprises at least three substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(464) F276A+K308E+K309E, F276A+K308E+K312E, F276A+K309E+K312E, K308E+K309E+K312E,
(465) N227T+F276A+K309E, N227T+F276A+K312E, N227T+K308E+K309E, N227T+K308E+K312E,
(466) N227T+K309E+K312E, N227T+N252P+K308E, N227T+N252P+K309E, N227T+N252P+K312E,
(467) N227T+N252P+N267T, N227T+N267T+F276A, N227T+N267T+K308E, N227T+N267T+K309E,
(468) N227T+N267T+K312E, N252P+F276A+K308E, N252P+F276A+K309E, N252P+F276A+K312E,
(469) N252P+K308E+K309E, N252P+K308E+K312E, N252P+N267T+F276A, N252P+N267T+K308E,
(470) N252P+N267T+K309E, N252P+N267T+K312E, N267T+F276A+K308E, N267T+F276A+K309E,
(471) N267T+F276A+K312E, N267T+K308E+K309E, N267T+K308E+K312E, N267T+K309E+K312E,
(472) Q215K+F276A+K308E, Q215K+F276A+K312E, Q215K+K308E+K312E, Q215K+K309E+K312E,
(473) Q215K+N227T+F276A, Q215K+N227T+K308E, Q215K+N227T+K309E, Q215K+N227T+K312E,
(474) Q215K+N227T+N252P, Q215K+N227T+N267T, Q215K+N252P+F276A, Q215K+N252P+K309E,
(475) Q215K+N252P+K312E, Q215K+N252P+N267T, Q215K+N267T+F276A, Q215K+N267T+K312E,
(476) S163P+F276A+K308E, S163P+F276A+K312E, S163P+K308E+K309E, S163P+K308E+K312E,
(477) S163P+K309E+K312E, S163P+N227T+F276A, S163P+N227T+K309E, S163P+N227T+N252P,
(478) S163P+N227T+N267T, S163P+N252P+F276A, S163P+N252P+K308E, S163P+N252P+K309E,
(479) S163P+N252P+K312E, S163P+N252P+N267T, S163P+N267T+K308E, S163P+N267T+K309E,
(480) S163P+N267T+K312E, S163P+Q215K+F276A, S163P+Q215K+K308E, S163P+Q215K+K309E,
(481) S163P+Q215K+K312E, S163P+Q215K+N227T and S163P Q215K N252P.
(482) In one embodiment, the dispersin variant comprises at least four substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(483) K312E+N227T+F276A+K308E, K312E+N227T+F276A+K309E, K312E+N227T+F276A+K312E,
(484) K312E+N227T+K308E+K309E, K312E+N227T+K308E+K312E, K312E+N227T+K309E+K312E,
(485) K312E+F276A+K308E+K309E, K312E+F276A+K308E+K312E, K312E+F276A+K309E+K312E,
(486) K312E+K308E+K309E+K312E, N227T+F276A+K308E+K309E, N227T+F276A+K308E+K312E,
(487) N227T+F276A+K309E+K312E, N227T+K308E+K309E+K312E, F276A+K308E+K309E+K312E,
(488) F276A+K308E+K309E+K312E, N227T+K308E+K309E+K312E and N227T+F276A+K308E+K309E.
(489) In one embodiment, the dispersin variant comprises at least four substitutions wherein the substitution sets are selected from the group consisting of:
(490) K312Q+N227T+S225G+K308Q, K312Q+N227T+S225G+K309E, K312Q+N227T+S225G+K312Q,
(491) K312Q+N227T+K308Q+K309E, K312Q+N227T+K308Q+K312Q, K312Q+N227T+K309E+K312Q,
(492) K312Q+S225G+K308Q+K309E, K312Q+S225G+K308Q+K312Q, K312Q+S225G+K309E+K312Q,
(493) K312Q+K308Q+K309E+K312Q, N227T+S225G+K308Q+K309E, N227T+S225G+K308Q+K312Q,
(494) N227T+S225G+K309E+K312Q, N227T+K308Q+K309E+K312Q, S225G+K308Q+K309E+K312Q,
(495) S225G+K308Q+K309E+K312Q, N227T+K308Q+K309E+K312Q and N227T+S225G+K308Q+K309E.
(496) In one embodiment, the dispersin variant comprises at least four substitutions wherein the substitutions or substitution sets are selected from the group consisting of: N227T+F276A+K308E+K309E, N227T+F276A+K308E+K312E, N227T+F276A+K309E+K312E, N227T+N267T+F276A+K308E, N227T+N267T+F276A+K309E, N227T+N267T+F276A+K312E, and S163P+N227T+F276A+K308E,
(497) In one embodiment, the dispersin variant comprises at least four substitutions wherein the substitution sets are selected from the group consisting of: N227T+S225G+K308Q+K309E, N227T+S225G+K308Q+K312Q, N227T+S225G+K309E+K312Q, N227T+N267T+S225G+K308Q, N227T+N267T+S225G+K309E, N227T+N267T+S225G+K312Q, and S163P+N227T+S225G+K308Q,
(498) In one embodiment, the dispersin variant comprises at least five substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(499) S163P+Q215K+N227T+N252P+N267T, S163P+Q215K+N227T+N252P+F276A,
(500) S163P+Q215K+N227T+N252P+K308E, S163P+Q215K+N227T+N252P+K309E,
(501) S163P+Q215K+N227T+N267T+F276A, S163P+Q215K+N227T+N267T+K308E,
(502) S163P+Q215K+N227T+N267T+K309E, S163P+Q215K+N227T+F276A+K308E,
(503) S163P+Q215K+N227T+F276A+K309E, S163P+Q215K+N227T+K308E+K309E,
(504) S163P+Q215K+N252P+N267T+F276A, S163P+Q215K+N252P+N267T+K308E,
(505) S163P+Q215K+N252P+N267T+K309E, S163P+Q215K+N252P+F276A+K308E,
(506) S163P+Q215K+N252P+F276A+K309E, S163P+Q215K+N252P+K308E+K309E,
(507) S163P+Q215K+N267T+F276A+K308E, S163P+Q215K+N267T+F276A+K309E,
(508) S163P+Q215K+N267T+K308E+K309E, S163P+Q215K+F276A+K308E+K309E,
(509) S163P+N227T+N252P+N267T+F276A, S163P+N227T+N252P+N267T+K308E,
(510) S163P+N227T+N252P+N267T+K309E, S163P+N227T+N252P+F276A+K308E,
(511) S163P+N227T+N252P+F276A+K309E, S163P+N227T+N252P+K308E+K309E,
(512) S163P+N227T+N267T+F276A+K308E, S163P+N227T+N267T+F276A+K309E,
(513) S163P+N227T+N267T+K308E+K309E, S163P+N227T+F276A+K308E+K309E,
(514) S163P+N252P+N267T+F276A+K308E, S163P+N252P+N267T+F276A+K309E,
(515) S163P+N252P+N267T+K308E+K309E, S163P+N252P+F276A+K308E+K309E,
(516) S163P+N267T+F276A+K308E+K309E, Q215K+N227T+N252P+N267T+F276A,
(517) Q215K+N227T+N252P+N267T+K308E, Q215K+N227T+N252P+N267T+K309E,
(518) Q215K+N227T+N252P+F276A+K308E, Q215K+N227T+N252P+F276A+K309E,
(519) Q215K+N227T+N252P+K308E+K309E, Q215K+N227T+N267T+F276A+K308E,
(520) Q215K+N227T+N267T+F276A+K309E, Q215K+N227T+N267T+K308E+K309E,
(521) Q215K+N227T+F276A+K308E+K309E, Q215K+N252P+N267T+F276A+K308E,
(522) Q215K+N252P+N267T+F276A+K309E, Q215K+N252P+N267T+K308E+K309E,
(523) Q215K+N252P+F276A+K308E+K309E, Q215K+N267T+F276A+K308E+K309E,
(524) N227T+N252P+N267T+F276A+K308E, N227T+N252P+N267T+F276A+K309E,
(525) N227T+N252P+N267T+K308E+K309E, N227T+N252P+F276A+K308E+K309E,
(526) N227T+N267T+F276A+K308E+K309E, N252P+N267T+F276A+K308E+K309E,
(527) Q215K+N227T+F276A+K308E+K309E, Q215K+N227T+F276A+K308E+K312E,
(528) Q215K+N227T+F276A+K309E+K312E, Q215K+N227T+K308E+K309E+K312E,
(529) Q215K+F276A+K308E+K309E+K312E, and N227T+F276A+K308E+K309E+K312E.
(530) In one embodiment, the dispersin variant comprises at least five substitutions wherein the substitution sets are selected from the group consisting of:
(531) S163P+N59E+N227T+N252P+E232D, S163P+N59E+N227T+N252P+S225G,
(532) S163P+N59E+N227T+N252P+K308Q, S163P+N59E+N227T+N252P+K309E,
(533) S163P+N59E+N227T+E232D+S225G, S163P+N59E+N227T+E232D+K308Q,
(534) S163P+N59E+N227T+E232D+K309E, S163P+N59E+N227T+S225G+K308Q,
(535) S163P+N59E+N227T+S225G+K309E, S163P+N59E+N227T+K308Q+K309E,
(536) S163P+N59E+N252P+E232D+S225G, S163P+N59E+N252P+E232D+K308Q,
(537) S163P+N59E+N252P+E232D+K309E, S163P+N59E+N252P+S225G+K308Q,
(538) S163P+N59E+N252P+S225G+K309E, S163P+N59E+N252P+K308Q+K309E,
(539) S163P+N59E+E232D+S225G+K308Q, S163P+N59E+E232D+S225G+K309E,
(540) S163P+N59E+E232D+K308Q+K309E, S163P+N59E+S225G+K308Q+K309E,
(541) S163P+N227T+N252P+E232D+S225G, S163P+N227T+N252P+E232D+K308Q,
(542) S163P+N227T+N252P+E232D+K309E, S163P+N227T+N252P+S225G+K308Q,
(543) S163P+N227T+N252P+S225G+K309E, S163P+N227T+N252P+K308Q+K309E,
(544) S163P+N227T+E232D+S225G+K308Q, S163P+N227T+E232D+S225G+K309E,
(545) S163P+N227T+E232D+K308Q+K309E, S163P+N227T+S225G+K308Q+K309E,
(546) S163P+N252P+E232D+S225G+K308Q, S163P+N252P+E232D+S225G+K309E,
(547) S163P+N252P+E232D+K308Q+K309E, S163P+N252P+S225G+K308Q+K309E,
(548) S163P+E232D+S225G+K308Q+K309E, N59E+N227T+N252P+E232D+S225G,
(549) N59E+N227T+N252P+E232D+K308Q, N59E+N227T+N252P+E232D+K309E,
(550) N59E+N227T+N252P+S225G+K308Q, N59E+N227T+N252P+S225G+K309E,
(551) N59E+N227T+N252P+K308Q+K309E, N59E+N227T+E232D+S225G+K308Q,
(552) N59E+N227T+E232D+S225G+K309E, N59E+N227T+E232D+K308Q+K309E,
(553) N59E+N227T+S225G+K308Q+K309E, N59E+N252P+E232D+S225G+K308Q,
(554) N59E+N252P+E232D+S225G+K309E, N59E+N252P+E232D+K308Q+K309E,
(555) N59E+N252P+S225G+K308Q+K309E, N59E+E232D+S225G+K308Q+K309E,
(556) N227T+N252P+E232D+S225G+K308Q, N227T+N252P+E232D+S225G+K309E,
(557) N227T+N252P+E232D+K308Q+K309E, N227T+N252P+S225G+K308Q+K309E,
(558) N227T+E232D+S225G+K308Q+K309E N252P+E232D+S225G+K308Q+K309E,
(559) N59E+N227T+S225G+K308Q+K309E, N59E+N227T+S225G+K308Q+K312Q,
(560) N59E+N227T+S225G+K309E+K312Q, N59E+N227T+K308Q+K309E+K312Q,
(561) N59E+S225G+K308Q+K309E+K312Q, and N227T+S225G+K308Q+K309E+K312Q.
(562) In one embodiment, the dispersin variant comprises at least five substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(563) N227T+N267T+F276A+K308E+K309E, N227T+N267T+F276A+K308E+K312E,
(564) N227T+N267T+F276A+K309E+K312E, S163P+N227T+F276A+K308E+K312E,
(565) N227T+F276A+K308E+K309E+K312E, N227T+F276A+K308E+K309E+K312E, and
(566) N227T+F276A+K308E+K309E+K312E.
(567) In one embodiment, the dispersin variant comprises at least five substitutions wherein the substitution sets are selected from the group consisting of:
(568) N227T+E232D+S225G+K308Q+K309E, N227T+E232D+S225G+K308Q+K312Q,
(569) N227T+E232D+S225G+K309E+K312Q, S163P+N227T+S225G+K308Q+K312Q,
(570) N227T+S225G+K308Q+K309E+K312Q, N227T+S225G+K308Q+K309E+K312Q, and
(571) N227T+S225G+K308Q+K309E+K312Q.
(572) In one embodiment, the dispersin variant comprises at least six substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(573) N227T+N267T+F276A+K308E+K309E+K312E, N227T+N267T+F276A+K308E+K309E+K312E,
(574) N227T+N252P+F276A+K308E+K309E+K312E, Q215K+N227T+F276A+K308E+K309E+K312E,
(575) S163P+N227T+F276A+K308E+K309E+K312E, N227T+N267T+F276A+K308E+K309E+K312E,
(576) N227T+N252P+F276A+K308E+K309E+K312E, Q215K+N227T+F276A+K308E+K309E+K312E and
(577) S163P+N227T+F276A+K308E+K309E+K312E.
(578) In one embodiment, the dispersin variant comprises at least six substitutions wherein the substitution sets are selected from the group consisting of:
(579) N227T+S225G+S186R+K308Q+K309E+K312Q, N227T+S225G+S186R+K308Q+K309E+K312Q,
(580) N227T+N252P+S186R+K308Q+K309E+K312Q, G235W+N227T+S186R+K308Q+K309E+K312Q,
(581) S163P+N227T+S186R+K308Q+K309E+K312Q, N227T+S225G+S186R+K308Q+K309E+K312Q,
(582) N227T+N252P+S186R+K308Q+K309E+K312Q, G235W+N227T+S186R+K308Q+K309E+K312Q and
(583) S163P+N227T+S186R+K308Q+K309E+K312Q.
(584) In one embodiment, the dispersin variant comprises at least seven substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(585) N227T+N252P+N267T+F276A+K308E+K309E+K312E,
(586) Q215K+N227T+N252P+F276A+K308E+K309E+K312E,
(587) Q215K+N227T+N267T+F276A+K308E+K309E+K312E,
(588) S163P+N227T+N252P+F276A+K308E+K309E+K312E,
(589) S163P+N227T+N267T+F276A+K308E+K309E+K312E,
(590) S163P+Q215K+N227T+F276A+K308E+K309E+K312E,
(591) N227T+N252P+N267T+F276A+K308E+K309E+K312E,
(592) Q215K+N227T+N252P+F276A+K308E+K309E+K312E,
(593) Q215K+N227T+N267T+F276A+K308E+K309E+K312E,
(594) S163P+N227T+N252P+F276A+K308E+K309E+K312E,
(595) S163P+N227T+N267T+F276A+K308E+K309E+K312E, and
(596) S163P+Q215K+N227T+F276A+K308E+K309E+K312E.
(597) In one embodiment, the dispersin variant comprises at least seven substitutions wherein the substitution sets are selected from the group consisting of:
(598) N227T+N252P+S225G+S186R+K308Q+K309E+K312Q,
(599) S186R+N227T+N252P+S186R+K308Q+K309E+K312Q,
(600) S186R+N227T+S225G+S186R+K308Q+K309E+K312Q,
(601) S163P+N227T+N252P+S186R+K308Q+K309E+K312Q,
(602) S163P+N227T+S225G+S186R+K308Q+K309E+K312Q,
(603) S163P+S186R+N227T+S186R+K308Q+K309E+K312Q,
(604) N227T+N252P+S225G+S186R+K308Q+K309E+K312Q,
(605) S186R+N227T+N252P+S186R+K308Q+K309E+K312Q,
(606) S186R+N227T+S225G+S186R+K308Q+K309E+K312Q,
(607) S163P+N227T+N252P+S186R+K308Q+K309E+K312Q,
(608) S163P+N227T+S225G+S186R+K308Q+K309E+K312Q, and
(609) S163P+S186R+N227T+S186R+K308Q+K309E+K312Q.
(610) In one embodiment, the dispersin variant comprises at least eight substitutions wherein the substitutions are selected from the group consisting of:
(611) S163P+Q215K+N227T+N252P+N267T+F276A+K308E+K309E,
(612) S163P+Q215K+N227T+N252P+N267T+F276A+K308E+K312E,
(613) S163P+Q215K+N227T+N252P+N267T+F276A+K309E+K312E,
(614) S163P+Q215K+N227T+N252P+N267T+K308E+K309E+K312E,
(615) S163P+Q215K+N227T+N252P+F276A+K308E+K309E+K312E,
(616) S163P+Q215K+N227T+N267T+F276A+K308E+K309E+K312E,
(617) S163P+Q215K+N252P+N267T+F276A+K308E+K309E+K312E,
(618) S163P+N227T+N252P+N267T+F276A+K308E+K309E+K312E, and
(619) Q215K+N227T+N252P+N267T+F276A+K308E+K309E+K312E.
(620) In one embodiment, the dispersin variant comprises at least eight substitutions wherein the substitution sets are selected from the group consisting of:
(621) S163P+G235W+N227T+N252P+S186R+N260Q+K308Q+K309E,
(622) S163P+G235W+N227T+N252P+S186R+N260Q+K308Q+K312Q,
(623) S163P+G235W+N227T+N252P+S186R+N260Q+K309E+K312Q,
(624) S163P+G235W+N227T+N252P+S186R+K308Q+K309E+K312Q,
(625) S163P+G235W+N227T+N252P+N260Q+K308Q+K309E+K312Q,
(626) S163P+G235W+N227T+S186R+N260Q+K308Q+K309E+K312Q,
(627) S163P+G235W+N252P+S186R+N260Q+K308Q+K309E+K312Q,
(628) S163P+N227T+N252P+S186R+N260Q+K308Q+K309E+K312Q, and
(629) G235W+N227T+N252P+S186R+N260Q+K308Q+K309E+K312Q.
(630) In one embodiment, the dispersin variant comprises at least eight substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(631) Q215K+N227T+N252P+N267T+F276A+K308E+K309E+K312E,
(632) S163P+N227T+N252P+N267T+F276A+K308E+K309E+K312E,
(633) S163P+Q215K+N227T+N252P+F276A+K308E+K309E+K312E,
(634) S163P+Q215K+N227T+N267T+F276A+K308E+K309E+K312E,
(635) Q215K+N227T+N252P+N267T+F276A+K308E+K309E+K312E,
(636) S163P+N227T+N252P+N267T+F276A+K308E+K309E+K312E,
(637) S163P+Q215K+N227T+N252P+F276A+K308E+K309E+K312E and
(638) S163P+Q215K+N227T+N267T+F276A+K308E+K309E+K312E.
(639) In one embodiment, the dispersin variant comprises at least eight substitutions wherein the substitution sets are selected from the group consisting of:
(640) S225G+N227T+N252P+S186R+N260Q+K308E+K309E+K312E,
(641) S163P+N227T+N252P+S186R+N260Q+K308E+K309E+K312E,
(642) S163P+S225G+N227T+N252P+N260Q+K308E+K309E+K312E,
(643) S163P+S225G+N227T+S186R+N260Q+K308E+K309E+K312E,
(644) S225G+N227T+N252P+S186R+N260Q+K308E+K309E+K312E,
(645) S163P+N227T+N252P+S186R+N260Q+K308E+K309E+K312E,
(646) S163P+S225G+N227T+N252P+N260Q+K308E+K309E+K312E and
(647) S163P+S225G+N227T+S186R+N260Q+K308E+K309E+K312E.
(648) In one embodiment, the dispersin variant comprises at least nine substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(649) S163P+Q215K+N227T+N252P+N267T+F276A+K308E+K309E+K312E,
(650) Q3F+Q215K+N227T+N252P+N267T+F276A+K308E+K309E+K312E,
(651) A49W+S163P+Q215K+N227T+N252P+N267T+F276A+K309E+K312E,
(652) Q3F+S163P+Q215K+N227T+N252P+N267T+K308E+K309E+K312E, and
(653) A49W+Q215K+N227T+N252P+N267T+F276A+K308E+K309E+K312E.
(654) In one embodiment, the dispersin variant comprises at least nine substitutions wherein the substitution sets are selected from the group consisting of:
(655) S163P+Q225G+N227T+N252P+N260Q+S186R+K308Q+K309E+K312Q,
(656) Q3I+Q225G+N227T+N252P+N260Q+S186R+K308Q+K309E+K312Q,
(657) A49W+S163P+Q225G+N227T+N252P+N260Q+S186R+K309E+K312Q,
(658) Q3I+S163P+Q225G+N227T+N252P+N260Q+K308Q+K309E+K312Q, and
(659) A49W+Q225G+N227T+N252P+N260Q+S186R+K308Q+K309E+K312Q.
(660) In one embodiment, the dispersin variant comprises at least nine substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(661) S163P+Q215K+N227T+N252P+N267T+F276A+K308E+K309E+K312E and
(662) S163P+Q215K+N227T+N252P+N267T+F276A+K308E+K309E+K312E.
(663) In one embodiment, the dispersin variant comprises at least nine substitutions wherein the substitution sets are selected from the group consisting of:
(664) S163P+S186R+S225G+N227T+G235W+N252P+K308Q+K309E+K312Q and
(665) S163P+S186R+S225G+N227T+G235W+N252P+K308Q+K309E+K312Q.
(666) In one embodiment, the dispersin variant comprises at least eight, at least nine, at least ten or at least eleven substitutions wherein the substitutions or substitution sets are selected from the group consisting of:
(667) Q3F+A49W+Q215K+F276A+K308E+K309E+K312E+N227T+N267T,
(668) Q3F+A49W+Q215K+F276A+K308E+K309E+K312E+N227T+N267T,
(669) Q3F+A49W+Q215K+F276A+K308E+K309E+K312E+N227T+N267T,
(670) Q3F+A49W+Q215K+F276A+K308E+K309E+K312E+N227T+,
(671) Q3F+A49W+Q215K+F276A+K308E+K309E+K312E+N267T+,
(672) Q3F+A49W+Q215K+F276A+K308E+K309E+N227T+N267T+,
(673) Q3F+A49W+Q215K+F276A+K308E+K312E+N227T+N267T+,
(674) Q3F+A49W+Q215K+F276A+K309E+K312E+N227T+N267T+,
(675) Q3F+A49W+Q215K+K308E+K309E+K312E+N227T+N267T+,
(676) Q3F+A49W+F276A+K308E+K309E+K312E+N227T+N267T+,
(677) Q3F+Q215K+F276A+K308E+K309E+K312E+N227T+N267T+,
(678) A49W+Q215K+F276A+K308E+K309E+K312E+N227T+N267T+, and
(679) Q3F+A49W+Q215K+F276A+K308E+K309E+K312E+N227T+N267T.
(680) In one embodiment, the dispersin variant comprises at least eleven substitutions wherein the substitution sets are selected from the group consisting of:
(681) Q3I+A49W+N59E+S163P+S186R+S225G+N227T+Y281P+K308Q+K309E+K312Q,
(682) Q3I+A49W+N59E+S163P+S186R+S225G+E232D+Y281P+K308Q+K309E+K312Q,
(683) Q3I+A49W+N59E+S163P+S186R+S225G+G235W+Y281P+K308Q+K309E+K312Q,
(684) Q3I+A49W+N59E+S163P+S186R+S225G+N252P+Y281P+K308Q+K309E+K312Q,
(685) Q3I+A49W+N59E+S163P+S186R+S225G+N260Q+Y281P+K308Q+K309E+K312Q,
(686) Q3I+A49W+N59E+S163P+S186R+S225G+N227T+H272V+Y281P+K308Q+K309E,
(687) Q3I+A49W+N59E+S163P+S186R+S225G+N227T+S279D+Y281P+K308Q+K312Q,
(688) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+Y281P+K309E+K312Q,
(689) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+G235W+K308Q+K309E+K312Q,
(690) H15Y+A49W+S163P+S186R+S225G+N227T+Y281P+S288P+K308Q+K309E+K312Q,
(691) H15Y+A49W+S163P+S186R+S225G+N227T+N252P+Y281P+K308Q+K309E+K312Q,
(692) H15Y+A49W+S163P+S186R+S225G+N227T+H272P+Y281P+K308Q+K309E+K312Q,
(693) H15Y+A49W+S163P+S186R+S225G+N227T+S279D+Y281P+K308Q+K309E+K312Q, and
(694) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+Y281P+K308Q+K312Q.
(695) In one embodiment, the dispersin variant comprises one of the substitution sets selected from the group consisting of:
(696) Q3F+A49W+N59E+S163P+S186R+S225G+N227T+N252P+F276A+Y281P+K308E+K309E+K312E;
(697) Q3I+H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+N260Q+N267T+H272V+S279D+Y281P+K308Q+K309E+K312Q;
(698) Q3I+H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+N260Q+N267T+H272P+S279D+Y281P+K308Q+K309E+K312Q;
(699) Q3I+H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+G235W+N252P+N260Q+H272V+S27 9D+Y281P+K308Q+K309E+K312E;
(700) Q3I+H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+G235W+N252P+N260Q+H272V+S27 9D+Y281P+K308E+K309E+K312Q;
(701) Q3F+H15Y+V140I+S163P+S186R+Q215K+S225G+N227T+E232D+G235W+N252P+N260Q+N267T+H2 72V+S279D+Y281P+K308Q+K309E+K312Q;
(702) Q3F+H15Y+S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N260Q+N267T+H272V+S279D+Y2 81P+K308Q+K309E+K312Q;
(703) Q3F+H15Y+T17W+A49W+N59E+S163P+S186R+T218Q+S225G+N227T+G235W+S237W+N252P+H27 2P+K308Q+K309E+K312Q;
(704) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+G235W+N252P+N260Q+H272V+S279D+Y 281P+K309E+K312Q;
(705) Q3F+H15Y+T17W+A49W+N59E+S163P+S186R+D207N+T218Q+S225G+N227T+G235W+S237W+N25 2P+H272P+Y281P+K308Q+K309E+K312Q;
(706) Q3I+H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+G235W+N252P+N260Q+H272V+S27 9D+Y281P+K308Q+K309E+K312Q;
(707) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+N260Q+H272P+S279D+Y281P+S 288P+K308Q+K309E+K312Q; and
(708) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+N260Q+H272P+S279D+Y281P+K 308Q+K309E+K312Q, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(709) In one embodiment, the dispersin variant comprises one of the substitution sets selected from the group consisting of: S186R+N227T+E232D+K308E,
(710) S163P+Q215K+N227T+N252P+N267T+F276A+K308Q+K309E+K312E,
(711) S163P+Q215K+N227T+N252P+N267T+F276A+K308E+K309E+K312Q,
(712) Q3F+A49W+N59E+S163P+S186R+N227T+E232D+N252P+F276A+S279G+K308E+K309E+K312E,
(713) A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+F276A+S279G+K308E+K309E+K312E,
(714) Q3F+T17W+A49W+N59E+S163P+S186R+S225G+N227T+G235W+N252P+H272P+Y281P+K308Q+K30 9E+K312Q,
(715) A49W+N59E+S163P+S186R+N227T+E232D+N252P+H272V+F276A+S279G+K308E+K309E+K312E,
(716) Q3F+A49W+N59E+S163P+S186R+S225G+N227T+N252P+F276A+S279G+K308E+K309E+K312E,
(717) A49W+N59E+S163P+S186R+N227T+E232D+N252P+F276A+S279G+Y281P+K308E+K309E+K312E,
(718) A49W+N59E+S163P+S186R+S225G+N227T+N252P+F276A+S279G+Y281P+K308E+K309E+K312E,
(719) Q3F+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+F276A+S279G+K308E+K309E+K31 2E,
(720) Q3F+S163P+S186R+Q215K+N227T+E232D+N252P+N267T+F276A+S279G+K308E+K309E+K312E,
(721) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+G235W+N252P+N260Q+H272P+S279D+Y 281P+K308Q+K309E+K312Q,
(722) Q3F+S163P+S186R+Q215K+S225G+N227T+N252P+N267T+F276A+S279G+K308E+K309E+K312E,
(723) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+N260Q+H272P+S279D+Y281P+S 288P+K308Q+K309E+K312Q,
(724) A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+H272V+F276A+S279G+K308E+K309E+K 312E,
(725) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+G235W+N252P+N260Q+H272V+S279D+Y 281P+K308Q+K309E+K312Q,
(726) S163P+S186R+Q215K+N227T+E232D+N252P+N267T+F276A+S279G+Y281P+K308E+K309E+K312E,
(727) Q3F+A49W+N59E+S163P+S186R+N227T+E232D+N252P+F276A+S279G+Y281P+K308E+K309E+K31 2E,
(728) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+N260Q+H272P+S279D+Y281P+K 308Q+K309E+K312Q,
(729) A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+F276A+S279G+Y281P+K308E+K309E+K 312E,
(730) H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+N260Q+H272V+Y281P+K308Q+K 309E+K312Q,
(731) Q3F+T17W+A49W+N59E+S163P+S186R+D207N+T218Q+S225G+N227T+G235W+N252P+H272P+Y2 81P+K308Q+K309E+K312Q,
(732) H15Y+T17W+A49W+N59E+S163P+S186R+S225G+N227T+E232D+G235W+N252P+N260Q+H272V+S 279D+Y281P+K308Q+K309E+K312Q,
(733) Q3F+A49W+N59E+S163P+S186R+S225G+N227T+N252P+F276A+S279G+Y281P+K308E+K309E+K31 2E,
(734) Q3F+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+H272V+F276A+S279G+K308E+K30 9E+K312E,
(735) A49W+N59E+S163P+S186R+N227T+E232D+N252P+H272V+F276A+S279G+Y281P+K308E+K309E+K 312E,
(736) S163P+S186R+S225G+N227T+E232D+N252P+N267T+F276A+S279G+Y281P+K308E+K309E+K312E,
(737) H15Y+T17W+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+N260Q+H272V+S279D+Y2 81P+K308Q+K309E+K312Q,
(738) Q3F+S163P+S186R+Q215K+N227T+E232D+N252P+N267T+H272V+F276A+S279G+K308E+K309E+K 312E,
(739) Q3I+H15Y+A49W+N59E+S163P+S186R+S225G+N227T+E232D+G235W+N252P+N260Q+H272V+S27 9D+Y281P+K308Q+K309E+K312Q,
(740) S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N267T+F276A+S279G+Y281P+K308E+K309E+K312E,
(741) S163P+Q215K+S225G+N227T+E232D+N252P+N267T+F276A+S279G+Y281P+K308E+K309E+K312E,
(742) S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N267T+H272V+F276A+S279G+K308E+K309E+K312E,
(743) S163P+Q215K+S225G+N227T+E232D+N252P+N267T+H272V+F276A+S279G+K308E+K309E+K312E,
(744) A49W+N59E+S163P+S186R+S225G+N227T+N252P+H272V+F276A+S279G+Y281P+K308E+K309E+K 312E,
(745) Q3F+S163P+S186R+Q215K+N227T+E232D+N252P+N267T+F276A+S279G+Y281P+K308E+K309E+K 312E,
(746) Q3F+S163P+Q215K+N227T+E232D+N252P+N267T+F276A+S279G+Y281P+K308E+K309E+K312E,
(747) Q3F+H15Y+T17W+A49W+N59E+S163P+S186R+T218Q+S225G+N227T+G235W+S237W+N252P+H27 2P+Y281P+K308Q+K309E+K312Q,
(748) S163P+Q215K+N227T+E232D+N252P+N267T+H272V+F276A+S279G+Y281P+K308E+K309E+K312E,
(749) Q3F+S163P+Q215K+S225G+N227T+E232D+N252P+N267T+H272V+F276A+S279G+K308E+K309E+K 312E,
(750) Q3F+A49W+N59E+S163P+S186R+N227T+E232D+N252P+H272V+F276A+S279G+Y281P+K308E+K30 9E+K312E,
(751) Q3F+S163P+S186R+Q215K+S225G+N227T+N252P+N267T+F276A+S279G+Y281P+K308E+K309E+K 312E,
(752) Q3F+H15Y+T17W+A49W+N59E+S163P+S186R+D207N+T218Q+S225G+N227T+G235W+S237W+N25 2P+H272P+Y281P+K308Q+K309E+K312Q,
(753) S163P+S186R+Q215K+S225G+N227T+N252P+N267T+H272V+F276A+S279G+Y281P+K308E+K309E+K312E,
(754) S163P+Q215K+S225G+N227T+N252P+N267T+H272V+F276A+S279G+Y281P+K308E+K309E+K312E,
(755) Q3F+S163P+Q215K+N227T+E232D+N252P+N267T+H272V+F276A+S279G+Y281P+K308E+K309E+K 312E,
(756) Q3F+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+F276A+S279G+Y281P+K308E+K30 9E+K312E,
(757) Q3F+A49W+N59E+S163P+S186R+S225G+N227T+N252P+H272V+F276A+S279G+Y281P+K308E+K30 9E+K312E,
(758) A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+H272V+F276A+S279G+Y281P+K308E+K 309E+K312E,
(759) S163P+Q215K+S225G+N227T+E232D+N252P+N267T+H272V+F276A+S279G+Y281P+K308E+K309E+K312E,
(760) Q3F+S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N260Q+N267T+H272P+S279D+Y281P+K 308Q+K309E+K312Q,
(761) Q3F+S163P+S186R+Q215K+N227T+E232D+N252P+N267T+H272V+F276A+S279G+Y281P+K308E+K 309E+K312E,
(762) S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N267T+H272V+F276A+S279G+Y281P+K308E+K309E+K312E,
(763) Q3F+S163P+S186R+Q215K+S225G+N227T+N252P+N267T+H272V+F276A+S279G+Y281P+K308E+K 309E+K312E,
(764) Q3F+H15Y+V140I+S163P+S186R+Q215K+S225G+N227T+E232D+G235W+N252P+N260Q+N267T+H2 72V+S279D+Y281P+K308Q+K309E+K312Q,
(765) Q3F+T17W+S163P+S186R+Q215K+S225G+N227T+E232D+G235W+N252P+N267T+H272V+F276A+S 279G+Y281P+K308E+K309E+K312Q,
(766) Q3F+S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N267T+H272V+F276A+S279G+Y281P+K 308E+K309E+K312Q,
(767) Q3F+A49W+N59E+S163P+S186R+S225G+N227T+E232D+N252P+H272V+F276A+S279G+Y281P+K30 8E+K309E+K312E,
(768) Q3F+S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N267T+H272V+F276A+S279G+Y281P+K 308E+K309E+K312E,
(769) Q3F+H15Y+T17W+S163P+S186R+Q215K+S225G+N227T+E232D+G235W+N252P+N260Q+N267T+H2 72V+S279D+Y281P+K308Q+K309E+K312Q,
(770) Q3F+S163P+Q215K+S225G+N227T+E232D+N252P+N267T+H272V+F276A+S279G+Y281P+K308E+K 309E+K312E,
(771) Q3F+H15Y+S163P+S186R+Q215K+S225G+N227T+E232D+G235W+N252P+N260Q+N267T+H272P+S 279D+Y281P+K308Q+K309E+K312Q,
(772) Q3F+A49W+N59E+S163P+S186R+S225G+N227T+E232D+G235W+N252P+N260Q+H272P+F276A+S2 79D+Y281P+K308E+K309E+K312Q,
(773) Q3F+H15Y+T17W+S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N260Q+N267T+H272V+S2 79D+Y281P+K308Q+K309E+K312Q,
(774) Q3F+H15Y+S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N260Q+N267T+H272P+S279D+Y2 81P+K308Q+K309E+K312Q,
(775) Q3F+H15Y+S163P+S186R+Q215K+S225G+N227T+E232D+G235W+N252P+N267T+H272V+F276A+S 279G+Y281P+K308Q+K309E+K312Q,
(776) Q3F+H15Y+T17W+S163P+S186R+Q215K+S225G+N227T+E232D+N252P+N267T+H272V+F276A+S27 9G+Y281P+K308Q+K309E+K312Q, and
(777) Q3F+H15Y+A49W+N59E+V140I+S163P+S186R+Q215K+S225G+N227T+E232D+G235W+N252P+N26 0Q+N267T+H272V+S279D+Y281P+K308Q+K309E+K312Q, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(778) In one embodiment, the dispersin variant comprises one of the substitution sets selected from the group consisting of: A49W+F276A+K308E; A49W+N227T+A269C+Y282C+K308E; A49W+N227T+F276A; A49W+N227T+F276A+K308E; A49W+S186R+N227T+F276A; A49W+S186R+N227T+F276A+K308E; A49W+S186R+N227T+K308E; A49W+Y124R+N227T+F276A+K308E; A49W+Y124R+S186R+N227T+F276A; A49W+Y124R+S186R+N227T+F276A+K308E; A49W+Y124R+S186R+N227T+K308E; S163P+N227T+F276A+K308E; S163P+Q215K+N227T+N252P+N267T+F276A; S163P+Q215K+N227T+N252P+N267T+F276A+K308E; S163P+Q215K+N227T+N252P+N267T+F276A+K309E; S163P+Q215K+N227T+N252P+N267T+F276A+K312E; S163P+Q215K+N252P; S186R+F276A+K308E; S186R+N227T+F276A; S186R+N227T+K308E; Y124R+S186R+N227T+A269C+F276A+Y282C+K308E; and
Y124R+S186R+N227T+F276A+K308E, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(779) The dispersin variants above preferably has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1.
(780) In one preferred embodiment the dispersin comprises the polypeptide shown in SEQ ID NO 17 or a polypeptide having at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity hereto.
(781) In some aspects, the dispersin variant comprises one or more substitution sets selected from the group consisting of: Q3F+A49W, Q3F+N59E, Q3F+S163P, Q3F+S186R, Q3F+Q215K, Q3F+S225G, Q3F+N227T, Q3F+N252P, Q3F+N267T, Q3F+F276A, Q3F+Y281P, Q3F+K308E, Q3F+K309E, Q3F+K312E, A49W+N59E, A49W+S163P, A49W+S186R, A49W+Q215K, A49W+S225G, A49W+N227T, A49W+N252P, A49W+N267T, A49W+F276A, A49W+Y281P, A49W+K308E, A49W+K309E, A49W+K312E, N59E+S163P, N59E+S186R, N59E+Q215K, N59E+S225G, N59E+N227T, N59E+N252P, N59E+N267T, N59E+F276A, N59E+Y281P, N59E+K308E, N59E+K309E, N59E+K312E, S163P+S186R, S163P+Q215K, S163P+S225G, S163P+N227T, S163P+N252P, S163P+N267T, S163P+F276A, S163P+Y281P, S163P+K308E, S163P+K309E, S163P+K312E, S186R+Q215K, S186R+S225G, S186R+N227T, S186R+N252P, S186R+N267T, S186R+F276A, S186R+Y281P, S186R+K308E, S186R+K309E, S186R+K312E, Q215K+S225G, Q215K+N227T, Q215K+N252P, Q215K+N267T, Q215K+F276A, Q215K+Y281P, Q215K+K308E, Q215K+K309E, Q215K+K312E, S225G+N227T, S225G+N252P, S225G+N267T, S225G+F276A, S225G+Y281P, S225G+K308E, S225G+K309E, S225G+K312E, N227T+N252P, N227T+N267T, N227T+F276A, N227T+Y281P, N227T+K308E, N227T+K309E, N227T+K312E, N252P+N267T, N252P+F276A, N252P+Y281P, N252P+K308E, N252P+K309E, N252P+K312E, N267T+F276A, N267T+Y281P, N267T+K308E, N267T+K309E, N267T+K312E, F276A+Y281P, F276A+K308E, F276A+K309E, F276A+K312E, Y281P+K308E, Y281P+K309E, Y281P+K312E, K308E+K309E, K308E+K312E and K309E+K312E, wherein each substitution provides a dispersin variant having an increase in stability measured as half-life improvement factor, HIF or residual activity ratio, RAR, of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the parent dispersin e.g. a dispersin comprising the polypeptide having the amino acid sequence shown in SEQ ID NO: 1, wherein the dispersin variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to the position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1).
(782) In one embodiment, the dispersin variant comprises at least one di-sulfide bridge. In one embodiment, the dispersin variant comprised in the cleaning compositions comprises at least one of the disulfide bridge: A269C-Y282C.
(783) In one embodiment the dispersin variant comprises any of the following substitutions and disulfide bridges: A49W+A269C+F276A+Y282C+K308E, A49W+N227T+A269C+F276A+Y282C, A49W+N227T+A269C+F276A+Y282C+K308E, A49W+S186R+N227T+A269C+F276A+Y282C, A49W+S186R+N227T+A269C+F276A+Y282C+K308E, A49W+S186R+N227T+A269C+Y282C+K308E, A49W+Y124R+N227T+A269C+F276A+Y282C+K308E, A49W+Y124R+S186R+N227T+A269C+F276A+Y282C, A49W+Y124R+S186R+N227T+A269C+F276A+Y282C+K308E, A49W+Y124R+S186R+N227T+A269C+Y282C+K308E, N227T+A269C+F276A+Y282C+K308E, S186R+A269C+F276A+Y282C+K308E, S186R+N227T+A269C+F276A+Y282C, S186R+N227T+A269C+F276A+Y282C+K308E, S186R+N227T+A269C+Y282C+K308E or Y124R+S186R+N227T+A269C+F276A+Y282C+K308E.
(784) Preferably the dispersin variants have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98% or 100% sequence identity to SEQ ID NO 1.
(785) The dispersin variant may also comprise any of the following alterations: D2E, D2S, D2T, Q3A, Q3*, T17A, E19T, E19H, E19W, E19L, K22R, K22N, K22M, K22S, D26*, S29G, Y30C, Y30S, Y30E, Y30G, N31W, N31G, G32N, N34*, N34M, N43D, N43F, E44N, E44S, E44I, S50C, E51D, Y52F, G54D, G54R, S56D, S56A, S57N, S57L, E58R, E58K, E58W, E58P, N59Q, N59K, N59I, N59G, T601, T60M, T60R, T60A, N61E, N62K, N62M, N62V, N62G, T63Y, T63I, T63K, K67W, N68M, N68A, L71K, L71Q, L71E, L71D, S72P, A75R, A75W, A75G, A75N, A75M, D79R, D79L, D79N, D79E, D79W, K801, K80D, K80Y, D81M, D81I, D81*, D81E, K95M, G96A, E99V, E99M, K102A, K102Q, K103D, K103C, K103P, K103T, K103N, K103I, K103S, K104A, K104V, K104*, D105*, V106I, V106P, V106*, V106S, V106F, K107R, K107G, K107N, K107H, K107Q, L108M, N110W, D111L, D111K, D111H, V113*, V113R, D115V, Y116*, Y116E, S117W, S117V, S117R, E118C, E118F, E118H, E119*, E119T, E119Y, E119M, E119H, Y124A, Y124S, Y124K, Y124I, Y124F, D125G, R127V, R127G, V128W, V128L, D131R, D131*, N134R, N134M, Q135H, Q135W, D138A, D138C, D138I, D138E, D138K, D142M, D142L, D142T, D142V, D142*, Y145A, Y145K, Y145R, Y145G, P147I, P147*, P147N, P147E, K148M, K148R, K148G, K148P, K148N, F149Y, F149E, E150I, G151M, G151R, G151V, G151T, G151E, G151F, K152V, K152W, K152S, G164F, G164Q, G164L, G164W, G164R, G164A, G164C, V167N, H168D, H168M, H168A, H168W, H168S, L170I, D171F, D171N, D171M, D174L, D174T, D174A, D174R, N177D, N177*, Q178E, Q178H, S181H, S181M, S181R, S181G, S181F, K184P, K184M, K184D, E185R, E185D, E185N, E185M, S186A, S186H, S186P, K187N, K187F, K187Y, E189M, S199I, S199E, S199N, S199A, S199R, E200Q, E200H, E200I, E200Y, A203N, A203I, N204A, N204Y, D207M, D207L, D207P, D207H, S208M, S208N, S208P, S208R, S217L, S217A, S217R, T218E, T218C, T218K, G222N, G222K, E224G, E224L, D230M, N233I, W234K, W234N, W234F, G235D, S237A, S237T, S237P, Y244V, L249D, L249I, L249T, S251Q, S251K, S251D, S251T, N252T, N252W, N252Y, G253E, G253*, G253R, G253I, G253A, F254T, F254K, F254N, F254V, F254A, F254*, T255E, Q256V, E257D, N260D, N260H, E261N, Q262L, Q262W, Q262H, N267V, W268Q, W268V, A271F, H272*, H272N, H272T, H272S, I278T, D280M, Y282P, Y282D, H283K, A284L, E286D, S288F, S288W, S288A, S288Q, S288E, E300N, E300L, H301L, H301V, H301M, T303D, T303M, T303E, T303N, Q307L, K308A, K308D, K309V, L311M, R319A or R319L, preferably wherein the dispersin variants have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98% or 100% sequence identity to SEQ ID NO 1.
(786) The dispersin variant may also comprise any of the following alterations: N31W, N31G, S50C, N59I, N59Q, G96A, Y124A, V128L, D138A, F149Y, S181H, K184P, K184M, E189M, N204A, D207N, S208A, Q262L, Q262W, H283K, E300N, T303G, T303C or K309V, preferably the dispersin variants have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98% or 100% sequence identity to SEQ ID NO 1.
(787) Examples of conservative substitutions are within the groups of basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine and valine), aromatic amino acids (phenylalanine, tryptophan and tyrosine), and small amino acids (glycine, alanine, serine, threonine and methionine). Amino acid substitutions that do not generally alter specific activity are known in the art and are described, for example, by H. Neurath and R. L. Hill, 1979, In, The Proteins, Academic Press, New York. Common substitutions are Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr, Ser/Asn, Ala/Val, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile, Leu/Val, Ala/Glu, and Asp/Gly.
(788) Essential amino acids in a polypeptide can be identified according to procedures known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis (Cunningham and Wells, 1989, Science 244: 1081-1085). In the latter technique, single alanine mutations are introduced at every residue in the molecule, and the resultant mutant molecules are tested for dispersin activity to identify amino acid residues that are critical to the activity of the molecule. See also, Hilton et al., 1996, J. Biol. Chem. 271: 4699-4708. The active site of the enzyme or other biological interaction can also be determined by physical analysis of structure, as determined by such techniques as nuclear magnetic resonance, crystallography, electron diffraction, or photoaffinity labeling, in conjunction with mutation of putative contact site amino acids. See, for example, de Vos et al., 1992, Science 255: 306-312; Smith et al., 1992, J. Mol. Biol. 224: 899-904; Wlodaver et al., 1992, FEBS Lett. 309: 59-64.
(789) Parent Dispersin
(790) Preferably, the dispersin parent is obtained from a microorganism and the dispersin is a microbial enzyme. The dispersin is preferably of fungal or bacterial origin. The dispersin parent is preferably obtainable from Terribacillus e.g. Terribacillus saccharophilus, Terribacillus goriensis or Terribacillus saccharophilus.
(791) The parent dispersin is preferably selected from the group consisting of: a) a polypeptide comprising the amino acid sequence shown in SEQ ID NO 1 or is a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98% or 100% sequence identity hereto, b) a polypeptide comprising the amino acid sequence shown in SEQ ID NO 2 or is a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98% or 100% sequence identity hereto, c) a polypeptide comprising the amino acid sequence shown in SEQ ID NO 3 or is a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98% or 100% sequence identity hereto, d) a polypeptide comprising the amino acid sequence shown in SEQ ID NO 4 or is a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98% or 100% sequence identity hereto, and e) a polypeptide comprising the amino acid sequence shown in SEQ ID NO 5 or is a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98% or 100% sequence identity hereto.
(792) The parent dispersins preferably belong to the Terribacillus clade and comprises one or more of the conserved motifs: GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14).
(793) An overview of the Terribacillus clade is provided in the table below. The Terribacillus clade comprises homologous sequences. The polypeptides with hexosaminidase activity having the mature amino acid sequences SEQ ID Nos. 1-5 can be pairwise aligned using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol. 48: 443-453). The percent identities resulting from such alignments are shown below.
(794) TABLE-US-00001 SEQ ID SEQ ID SEQ ID SEQ ID SEQ ID NO 5 NO 4 NO 3 NO 2 NO 1 100 75.9 81.8 82.4 81.2 SEQ ID NO 5 75.9 100 77.5 76.5 77.5 SEQ ID NO 4 81.8 77.5 100 95.1 95.7 SEQ ID NO 3 82.4 76.5 95.1 100 93.5 SEQ ID NO 2 81.2 77.5 95.7 93.5 100 SEQ ID NO 1
(795) The table shows that the polypeptides share close sequence relatedness. The polypeptides comprising the amino acids sequences of SEQ ID NO 1, SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4 and SEQ ID NO 5 belongs to a subclade of the Dispersin clade. These polypeptides share more than 90% pairwise sequence identity and are close related to each other.
(796) The polypeptides comprised in the cleaning compositions all lies within the same clade, the Terribacillus clade, and all have common functional features including cleaning properties in the presence of detergents. In one aspect the parent dispersins belongs to the Terribacillus clade and comprises one or more of the motif(s) GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] (SEQ ID NO: 10), WND[SQR][IVL][TLVM] (SEQ ID NO: 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO: 13) or NLD[DR]S (SEQ ID NO: 14).
(797) Use and Methods
(798) The dispersin variants described herein may be added to and thus become a component of a cleaning e.g. detergent composition, as defined herein.
(799) The cleaning composition, if not indicated otherwise, may be formulated, for example, as a hand or machine laundry detergent composition including a laundry additive composition suitable for pre-treatment of stained fabrics and a rinse added fabric softener composition, or be formulated as a detergent composition for use in general household hard surface cleaning operations, or be formulated for hand or machine dishwashing operations.
(800) The cleaning compositions described herein may be used for cleaning an item such as a textile or a hard surface in e.g. laundry and dish wash processes.
(801) One aspect relates to a method of cleaning an item comprising a) adding a dispersin variant as described herein to a cleaning composition to obtain a cleaning composition, as defined above, and b) washing item with the composition, wherein the item is a textile or a hard surface.
(802) One aspect relates to a method of cleaning an item comprising; a) adding a dispersin variant, comprising an alteration, preferably substitution at one or more positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323 of the polypeptide of SEQ ID NO: 1, wherein the dispersin variant has beta-1,6 N-acetylglucosaminidase activity, wherein the dispersin variant has at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, to a cleaning composition to obtain a cleaning composition, as defined above, and b) washing item with the composition, wherein the item is a textile or a hard surface.
(803) One aspect relates to a method of cleaning an item comprising; a) adding a dispersin variant, comprising an alteration, preferably substitution at one or more positions corresponding to positions 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299 of the polypeptide of SEQ ID NO: 1, wherein the dispersin variant has beta-1,6 N-acetylglucosaminidase activity, wherein the dispersin variant has at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, to a cleaning composition to obtain a cleaning composition, as defined above, and b) washing item with the composition, wherein the item is a textile or a hard surface.
(804) One aspect relates to a method of cleaning an item comprising a) adding a dispersin variant, wherein the variant comprises any of the alterations selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124H, Y124I, Y124K, Y124L, Y124M, Y124N, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125H, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128A, V128C, V128D, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138N, D138Q, D138R, D138S, D138V, D138W, E139W, V140I, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150A, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151D, G151L, G151N, G151P, G151S, G151W, K152D, K152L, K152R, S163P, G164D, G164E, G164H, G164S, G164V, V167A, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171E, D171K, D171L, D171M, D171Q, D171R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181D, S181F, S181G, S181K, S181N, S181P, S181Q, S181T, S181V, S181W, E185A, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203Q, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207K, D207N, D207Q, D207R, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215K, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, S221N, G222D, E224A, E224P, S225G, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230N, D230R, D230T, D230W, E232D, E232V, N233D, N233E, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251A, S251L, S251N, S251R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256D, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260T, N260V, N260W, N260Y, E261*, E261A, E261D, E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, D264N, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288P, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308E, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309E, K309G, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312E, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, Y321F and N323R compared to the dispersin shown in SEQ ID NO 1 to a cleaning composition to obtain a cleaning composition, as defined above, and b) washing item with the composition, wherein the item is a textile or a hard surface.
(805) The cleaning compositions comprising dispersin variants are suitable for use in cleaning such as laundry. Thus, some aspect relates to a method for laundering an item, wherein the method comprises the steps of: a. Exposing an item to a wash liquor comprising a cleaning composition; b. Completing at least one wash cycle; and c. Optionally rinsing the item, wherein the item is a textile or a hard surface.
(806) The pH of the liquid solution is in the range of 1 to 11, such as in the range 5.5 to 11, such as in the range of 7 to 9, in the range of 7 to 8 or in the range of 7 to 8.5.
(807) The wash liquor may have a temperature in the range of 5° C. to 95° C., or in the range of 10° C. to 80° C., in the range of 10° C. to 70° C., in the range of 10° C. to 60° C., in the range of 10° C. to 50° C., in the range of 15° C. to 40° C. or in the range of 20° C. to 30° C. In some aspects the temperature of the wash liquor is 30° C.
(808) The concentration of the dispersin variant enzyme in the wash liquor is typically in the range of 0.00001-1000 ppm enzyme protein, such as in the range of 0.00004-100 ppm enzyme protein, such as in the range of 0.00008-100 enzyme protein, in the range of 0.0001-100 enzyme protein, in the range of 0.0002-100 enzyme protein, in the range of 0.0004-100 enzyme protein, in the range of 0.0008-100 enzyme protein, in the range of 0.001-100 ppm enzyme protein, in the range of 0.01-100 ppm enzyme protein, in the range of 1-1000 ppm enzyme protein, preferably in the range of 0.05-50 ppm enzyme protein, more preferably in the range of 0.1-50 ppm enzyme protein, more preferably in the range of 0.1-30 ppm enzyme protein, more preferably in the range of 0.5-20 ppm enzyme protein, and most preferably in the range of 0.5-10 ppm enzyme protein.
(809) In some aspects the dispersin variants comprised in the compositions are effective in preventing and/or reducing the malodor. The presence of complex organic stains such as body soil e.g. cell debris, sebum or EPS attach to e.g. laundry items which become sticky and therefore soil adheres to the sticky areas. This soil sticked to the laundry has shown difficulty to be removed by commercially available detergent compositions. Further, when dirty laundry items are washed together with less dirty laundry items the dirt present in the wash liquor tend to adhere to the laundry (e.g. by re-deposition) in particular if the laundry is sticky as described above. As a result, hereof, the laundry item is more “soiled” after wash than before wash. In some aspects, the dispersin variants have improved cleaning properties compared to the parent dispersin and in some aspects, the dispersin variants reduce stickiness and/or re-deposition.
(810) In some aspects, the use of a cleaning composition may include a dispersin variant for cleaning of an item, wherein the item is a fabric or a hard surface.
(811) Further, a cleaning composition as defined herein may include a dispersin variant for preventing and/or reducing the adherence of soil to an item. In some aspect, the item is textile. When the soil does not adhere to the item, the item appears cleaner. Thus, a cleaning composition as defined herein may include a dispersin variant for maintaining or improving the whiteness of the item.
(812) The cleaning composition defined herein comprising a dispersin variant may be used for deep cleaning of an item, for preventing and/or reducing the stickiness of an item, for pretreating stains on the item, for preventing and/or reducing redeposition of soil during a wash cycle, for preventing and/or reducing adherence of soil to an item, for maintaining or improving the whiteness of an item and/or for preventing and/or reducing malodor from an item.
(813) Nucleic Acid Constructs
(814) The present disclosure also relates to nucleic acid constructs comprising a polynucleotide encoding the dispersin variants operably linked to one or more control sequences that direct the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences.
(815) The polynucleotide may be manipulated in a variety of ways to provide for expression of the polypeptide. Manipulation of the polynucleotide prior to its insertion into a vector may be desirable or necessary depending on the expression vector. The techniques for modifying polynucleotides utilizing recombinant DNA methods are well known in the art.
(816) The control sequence may be a promoter, a polynucleotide that is recognized by a host cell for expression of a polynucleotide encoding a polypeptide. The promoter contains transcriptional control sequences that mediate the expression of the polypeptide. The promoter may be any polynucleotide that shows transcriptional activity in the host cell including mutant, truncated, and hybrid promoters, and may be obtained from genes encoding extracellular or intracellular polypeptides either homologous or heterologous to the host cell.
(817) Examples of suitable promoters for directing transcription of the nucleic acid constructs in a bacterial host cell are the promoters obtained from the Bacillus amyloliquefaciens alpha-amylase gene (amyQ), Bacillus licheniformis alpha-amylase gene (amyL), Bacillus licheniformis penicillinase gene (penP), Bacillus stearothermophilus maltogenic amylase gene (amyM), Bacillus subtilis levansucrase gene (sacB), Bacillus subtilis xyIA and xyIB genes, Bacillus thuringiensis cryIIIA gene (Agaisse and Lereclus, 1994, Molecular Microbiology 13: 97-107), E. coli lac operon, E. coli trc promoter (Egon et al., 1988, Gene 69: 301-315), Streptomyces coelicolor agarase gene (dagA), and prokaryotic beta-lactamase gene (Villa-Kamaroff et al., 1978, Proc. Natl. Acad. Sci. USA 75: 3727-3731), as well as the tac promoter (DeBoer et al., 1983, Proc. Natl. Acad. Sci. USA 80: 21-25). Further promoters are described in “Useful proteins from recombinant bacteria” in Gilbert et al., 1980, Scientific American 242: 74-94; and in Sambrook et al., 1989, supra. Examples of tandem promoters are disclosed in WO 99/43835.
(818) Examples of suitable promoters for directing transcription of the polynucleotide in a filamentous fungal host cell are promoters obtained from the genes for Aspergillus nidulans acetamidase, Aspergillus niger neutral alpha-amylase, Aspergillus niger acid stable alpha-amylase, Aspergillus niger or Aspergillus awamori glucoamylase (glaA), Aspergillus oryzae TAKA amylase, Aspergillus oryzae alkaline protease, Aspergillus oryzae triose phosphate isomerase, Fusarium oxysporum trypsin-like protease (WO 96/00787), Fusarium venenatum amyloglucosidase (WO 00/56900), Fusarium venenatum Daria (WO 00/56900), Fusarium venenatum Quinn (WO 00/56900), Rhizomucor miehei lipase, Rhizomucor miehei aspartic proteinase, Trichoderma reesei beta-glucosidase, Trichoderma reesei cellobiohydrolase I, Trichoderma reesei cellobiohydrolase II, Trichoderma reesei endoglucanase I, Trichoderma reesei endoglucanase II, Trichoderma reesei endoglucanase III, Trichoderma reesei endoglucanase V, Trichoderma reesei xylanase I, Trichoderma reesei xylanase II, Trichoderma reesei xylanase III, Trichoderma reesei beta-xylosidase, and Trichoderma reesei translation elongation factor, as well as the NA2 tpi promoter (a modified promoter from an Aspergillus neutral alpha-amylase gene in which the untranslated leader has been replaced by an untranslated leader from an Aspergillus triose phosphate isomerase gene; non-limiting examples include modified promoters from an Aspergillus niger neutral alpha-amylase gene in which the untranslated leader has been replaced by an untranslated leader from an Aspergillus nidulans or Aspergillus oryzae triose phosphate isomerase gene); and mutant, truncated, and hybrid promoters thereof. Other promoters are described in U.S. Pat. No. 6,011,147.
(819) In a yeast host, useful promoters are obtained from the genes for Saccharomyces cerevisiae enolase (ENO 1), Saccharomyces cerevisiae galactokinase (GAL1), Saccharomyces cerevisiae alcohol dehydrogenase/glyceraldehyde-3 phosphate dehydrogenase (ADH1, ADH2/GAP), Saccharomyces cerevisiae triose phosphate isomerase (TPI), Saccharomyces cerevisiae metallothionein (CUP1), and Saccharomyces cerevisiae 3 phosphoglycerate kinase. Other useful promoters for yeast host cells are described by Romanos et al., 1992, Yeast 8: 423-488.
(820) The control sequence may also be a transcription terminator, which is recognized by a host cell to terminate transcription. The terminator is operably linked to the 3′ terminus of the polynucleotide encoding the polypeptide. Any terminator that is functional in the host cell may be used.
(821) Preferred terminators for bacterial host cells are obtained from the genes for Bacillus clausii alkaline protease (aprH), Bacillus licheniformis alpha-amylase (amyL), and Escherichia coli ribosomal RNA (rrnB).
(822) Preferred terminators for filamentous fungal host cells are obtained from the genes for Aspergillus nidulans acetamidase, Aspergillus nidulans anthranilate synthase, Aspergillus niger glucoamylase, Aspergillus niger alpha-glucosidase, Aspergillus oryzae TAKA amylase, Fusarium oxysporum trypsin-like protease, Trichoderma reesei beta-glucosidase, Trichoderma reesei cellobiohydrolase I, Trichoderma reesei cellobiohydrolase II, Trichoderma reesei endoglucanase I, Trichoderma reesei endoglucanase II, Trichoderma reesei endoglucanase III, Trichoderma reesei endoglucanase V, Trichoderma reesei xylanase I, Trichoderma reesei xylanase II, Trichoderma reesei xylanase III, Trichoderma reesei beta-xylosidase, and Trichoderma reesei translation elongation factor.
(823) Preferred terminators for yeast host cells are obtained from the genes for Saccharomyces cerevisiae enolase, Saccharomyces cerevisiae cytochrome C (CYC1), and Saccharomyces cerevisiae glyceraldehyde-3 phosphate dehydrogenase. Other useful terminators for yeast host cells are described by Romanos et al., 1992, supra.
(824) The control sequence may also be an mRNA stabilizer region downstream of a promoter and upstream of the coding sequence of a gene which increases expression of the gene.
(825) Examples of suitable mRNA stabilizer regions are obtained from a Bacillus thuringiensis cryIIIA gene (WO 94/25612) and a Bacillus subtilis SP82 gene (Hue et al., 1995, J. Bacteriol. 177: 3465-3471).
(826) The control sequence may also be a leader, a nontranslated region of an mRNA that is important for translation by the host cell. The leader is operably linked to the 5′ terminus of the polynucleotide encoding the polypeptide. Any leader that is functional in the host cell may be used.
(827) Preferred leaders for filamentous fungal host cells are obtained from the genes for Aspergillus oryzae TAKA amylase and Aspergillus nidulans triose phosphate isomerase.
(828) Suitable leaders for yeast host cells are obtained from the genes for Saccharomyces cerevisiae enolase (ENO-1), Saccharomyces cerevisiae 3-phosphoglycerate kinase, Saccharomyces cerevisiae alpha-factor, and Saccharomyces cerevisiae alcohol dehydrogenase/glyceraldehyde-3-phosphate dehydrogenase (ADH2/GAP).
(829) The control sequence may also be a polyadenylation sequence, a sequence operably linked to the 3′ terminus of the polynucleotide and, when transcribed, is recognized by the host cell as a signal to add polyadenosine residues to transcribed mRNA. Any polyadenylation sequence that is functional in the host cell may be used.
(830) Preferred polyadenylation sequences for filamentous fungal host cells are obtained from the genes for Aspergillus nidulans anthranilate synthase, Aspergillus niger glucoamylase, Aspergillus niger alpha-glucosidase, Aspergillus oryzae TAKA amylase, and Fusarium oxysporum trypsin-like protease.
(831) Useful polyadenylation sequences for yeast host cells are described by Guo and Sherman, 1995, Mol. Cellular Biol. 15: 5983-5990.
(832) The control sequence may also be a signal peptide coding region that encodes a signal peptide linked to the N terminus of a polypeptide and directs the polypeptide into the cell's secretory pathway. The 5′ end of the coding sequence of the polynucleotide may inherently contain a signal peptide coding sequence naturally linked in translation reading frame with the segment of the coding sequence that encodes the polypeptide. Alternatively, the 5′ end of the coding sequence may contain a signal peptide coding sequence that is foreign to the coding sequence. A foreign signal peptide coding sequence may be required where the coding sequence does not naturally contain a signal peptide coding sequence. Alternatively, a foreign signal peptide coding sequence may simply replace the natural signal peptide coding sequence in order to enhance secretion of the polypeptide. However, any signal peptide coding sequence that directs the expressed polypeptide into the secretory pathway of a host cell may be used.
(833) Effective signal peptide coding sequences for bacterial host cells are the signal peptide coding sequences obtained from the genes for Bacillus NCIB 11837 maltogenic amylase, Bacillus licheniformis subtilisin, Bacillus licheniformis beta-lactamase, Bacillus stearothermophilus alpha-amylase, Bacillus stearothermophilus neutral proteases (nprT, nprS, nprM), and Bacillus subtilis prsA. Further signal peptides are described by Simonen and Palva, 1993, Microbiological Reviews 57: 109-137.
(834) Effective signal peptide coding sequences for filamentous fungal host cells are the signal peptide coding sequences obtained from the genes for Aspergillus niger neutral amylase, Aspergillus niger glucoamylase, Aspergillus oryzae TAKA amylase, Humicola insolens cellulase, Humicola insolens endoglucanase V, Humicola lanuginosa lipase, and Rhizomucor miehei aspartic proteinase.
(835) Useful signal peptides for yeast host cells are obtained from the genes for Saccharomyces cerevisiae alpha-factor and Saccharomyces cerevisiae invertase. Other useful signal peptide coding sequences are described by Romanos et al., 1992, supra.
(836) The control sequence may also be a propeptide coding sequence that encodes a propeptide positioned at the N terminus of a polypeptide. The resultant polypeptide is known as a proenzyme or propolypeptide (or a zymogen in some cases). A propolypeptide is generally inactive and can be converted to an active polypeptide by catalytic or autocatalytic cleavage of the propeptide from the propolypeptide. The propeptide coding sequence may be obtained from the genes for Bacillus subtilis alkaline protease (aprE), Bacillus subtilis neutral protease (nprT), Myceliophthora thermophila laccase (WO 95/33836), Rhizomucor miehei aspartic proteinase, and Saccharomyces cerevisiae alpha-factor.
(837) Where both signal peptide and propeptide sequences are present, the propeptide sequence is positioned next to the N terminus of a polypeptide and the signal peptide sequence is positioned next to the N terminus of the propeptide sequence.
(838) It may also be desirable to add regulatory sequences that regulate expression of the polypeptide relative to the growth of the host cell. Examples of regulatory sequences are those that cause expression of the gene to be turned on or off in response to a chemical or physical stimulus, including the presence of a regulatory compound. Regulatory sequences in prokaryotic systems include the lac, tac, and trp operator systems. In yeast, the ADH2 system or GAL1 system may be used. In filamentous fungi, the Aspergillus niger glucoamylase promoter, Aspergillus oryzae TAKA alpha-amylase promoter, and Aspergillus oryzae glucoamylase promoter, Trichoderma reesei cellobiohydrolase I promoter, and Trichoderma reesei cellobiohydrolase II promoter may be used. Other examples of regulatory sequences are those that allow for gene amplification. In eukaryotic systems, these regulatory sequences include the dihydrofolate reductase gene that is amplified in the presence of methotrexate, and the metallothionein genes that are amplified with heavy metals. In these cases, the polynucleotide encoding the polypeptide would be operably linked to the regulatory sequence.
(839) Expression Vectors
(840) The present disclosure also relates to recombinant expression vectors comprising a polynucleotide encoding the dispersin variants, a promoter, and transcriptional and translational stop signals. The various nucleotide and control sequences may be joined together to produce a recombinant expression vector that may include one or more convenient restriction sites to allow for insertion or substitution of the polynucleotide encoding the polypeptide at such sites. Alternatively, the polynucleotide may be expressed by inserting the polynucleotide or a nucleic acid construct comprising the polynucleotide into an appropriate vector for expression. In creating the expression vector, the coding sequence is located in the vector so that the coding sequence is operably linked with the appropriate control sequences for expression. In a further aspect, polynucleotide sequence codons have been modified by nucleotide substitutions to correspond to the codon usage of the host organism intended for production of the polypeptide. The recombinant expression vector may be any vector (e.g., a plasmid or virus) that can be conveniently subjected to recombinant DNA procedures and can bring about expression of the polynucleotide. The choice of the vector will typically depend on the compatibility of the vector with the host cell into which the vector is to be introduced. The vector may be a linear or closed circular plasmid.
(841) The vector may be an autonomously replicating vector, i.e., a vector that exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g., a plasmid, an extrachromosomal element, a minichromosome, or an artificial chromosome. The vector may contain any means for assuring self-replication. Alternatively, the vector may be one that, when introduced into the host cell, is integrated into the genome and replicated together with the chromosome(s) into which it has been integrated. Furthermore, a single vector or plasmid or two or more vectors or plasmids that together contain the total DNA to be introduced into the genome of the host cell, or a transposon, may be used.
(842) The vector preferably contains one or more selectable markers that permit easy selection of transformed, transfected, transduced, or the like cells. A selectable marker is a gene the product of which provides for biocide or viral resistance, resistance to heavy metals, prototrophy to auxotrophs, and the like.
(843) Examples of bacterial selectable markers are Bacillus licheniformis or Bacillus subtilis dal genes, or markers that confer antibiotic resistance such as ampicillin, chloramphenicol, kanamycin, neomycin, spectinomycin, or tetracycline resistance. Suitable markers for yeast host cells include, but are not limited to, ADE2, HIS3, LEU2, LYS2, MET3, TRP1, and URA3. Selectable markers for use in a filamentous fungal host cell include, but are not limited to, adeA (phosphoribosylaminoimidazole-succinocarboxamide synthase), adeB (phosphoribosyl-aminoimidazole synthase), amdS (acetamidase), argB (ornithine carbamoyltransferase), bar (phosphinothricin acetyltransferase), hph (hygromycin phosphotransferase), niaD (nitrate reductase), pyrG (orotidine-5′-phosphate decarboxylase), sC (sulfate adenyltransferase), and trpC (anthranilate synthase), as well as equivalents thereof. Preferred for use in an Aspergillus cell are Aspergillus nidulans or Aspergillus oryzae amdS and pyrG genes and a Streptomyces hygroscopicus bar gene. Preferred for use in a Trichoderma cell are adeA, adeB, amdS, hph, and pyrG genes.
(844) The selectable marker may be a dual selectable marker system as described in WO 2010/039889. In some aspects, the dual selectable marker is an hph-tk dual selectable marker system.
(845) The vector preferably contains an element(s) that permits integration of the vector into the host cell's genome or autonomous replication of the vector in the cell independent of the genome.
(846) For integration into the host cell genome, the vector may rely on the polynucleotide's sequence encoding the polypeptide or any other element of the vector for integration into the genome by homologous or non-homologous recombination. Alternatively, the vector may contain additional polynucleotides for directing integration by homologous recombination into the genome of the host cell at a precise location(s) in the chromosome(s). To increase the likelihood of integration at a precise location, the integrational elements should contain a sufficient number of nucleic acids, such as 100 to 10,000 base pairs, 400 to 10,000 base pairs, and 800 to 10,000 base pairs, which have a high degree of sequence identity to the corresponding target sequence to enhance the probability of homologous recombination. The integrational elements may be any sequence that is homologous with the target sequence in the genome of the host cell. Furthermore, the integrational elements may be non-encoding or encoding polynucleotides. On the other hand, the vector may be integrated into the genome of the host cell by non-homologous recombination.
(847) For autonomous replication, the vector may further comprise an origin of replication enabling the vector to replicate autonomously in the host cell in question. The origin of replication may be any plasmid replicator mediating autonomous replication that functions in a cell. The term “origin of replication” or “plasmid replicator” means a polynucleotide that enables a plasmid or vector to replicate in vivo.
(848) Examples of bacterial origins of replication are the origins of replication of plasmids pBR322, pUC19, pACYC177, and pACYC184 permitting replication in E. coli, and pUB110, pE194, pTA1060, and pAMß1 permitting replication in Bacillus.
(849) Examples of origins of replication for use in a yeast host cell are the 2 micron origin of replication, ARS1, ARS4, the combination of ARS1 and CEN3, and the combination of ARS4 and CEN6.
(850) Examples of origins of replication useful in a filamentous fungal cell are AMA1 and ANSI (Gems et al., 1991, Gene 98: 61-67; Cullen et al., 1987, Nucleic Acids Res. 15: 9163-9175; WO 00/24883). Isolation of the AMA1 gene and construction of plasmids or vectors comprising the gene can be accomplished according to the methods disclosed in WO 00/24883.
(851) More than one copy of a polynucleotide may be inserted into a host cell to increase production of a polypeptide. An increase in the copy number of the polynucleotide can be obtained by integrating at least one additional copy of the sequence into the host cell genome or by including an amplifiable selectable marker gene with the polynucleotide where cells containing amplified copies of the selectable marker gene, and thereby additional copies of the polynucleotide, can be selected for by cultivating the cells in the presence of the appropriate selectable agent.
(852) The procedures used to ligate the elements described above to construct the recombinant expression vectors are well known to one skilled in the art (see, e.g., Sambrook et al., 1989, supra).
(853) Host Cells
(854) The present disclosure also relates to recombinant host cells, comprising a polynucleotide operably linked to one or more control sequences that direct the production of a polypeptide. A construct or vector comprising a polynucleotide is introduced into a host cell so that the construct or vector is maintained as a chromosomal integrant or as a self-replicating extra-chromosomal vector as described earlier. The term “host cell” encompasses any progeny of a parent cell that is not identical to the parent cell due to mutations that occur during replication. The choice of a host cell will to a large extent depend upon the gene encoding the polypeptide and its source.
(855) The host cell may be any cell useful in the recombinant production of a polypeptide, e.g., a prokaryote or a eukaryote.
(856) The prokaryotic host cell may be any Gram-positive or Gram-negative bacterium. Gram-positive bacteria include, but are not limited to, Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus, and Streptomyces. Gram-negative bacteria include, but are not limited to, Campylobacter, E. coli, Flavobacterium, Fusobacterium, Helicobacter, Ilyobacter, Neisseria, Pseudomonas, Salmonella, and Ureaplasma.
(857) The bacterial host cell may be any Bacillus cell including, but not limited to, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophilus, Bacillus subtilis, and Bacillus thuringiensis cells.
(858) The bacterial host cell may also be any Streptococcus cell including, but not limited to, Streptococcus equisimilis, Streptococcus pyogenes, Streptococcus uberis, and Streptococcus equi subs p. Zooepidemicus cells.
(859) The bacterial host cell may also be any Streptomyces cell including, but not limited to, Streptomyces achromogenes, Streptomyces avermitilis, Streptomyces coelicolor, Streptomyces griseus, and Streptomyces lividans cells.
(860) The introduction of DNA into a Bacillus cell may be effected by protoplast transformation (see, e.g., Chang and Cohen, 1979, Mol. Gen. Genet. 168: 111-115), competent cell transformation (see, e.g., Young and Spizizen, 1961, J. Bacteriol. 81: 823-829, or Dubnau and Davidoff-Abelson, 1971, J. Mol. Biol. 56: 209-221), electroporation (see, e.g., Shigekawa and Dower, 1988, Biotechniques 6: 742-751), or conjugation (see, e.g., Koehler and Thorne, 1987, J. Bacteriol. 169: 5271-5278). The introduction of DNA into an E. coli cell may be effected by protoplast transformation (see, e.g., Hanahan, 1983, J. Mol. Biol. 166: 557-580) or electroporation (see, e.g., Dower et al., 1988, Nucleic Acids Res. 16: 6127-6145). The introduction of DNA into a Streptomyces cell may be effected by protoplast transformation, electroporation (see, e.g., Gong et al., 2004, Folia Microbiol. (Praha) 49: 399-405), conjugation (see, e.g., Mazodier et al., 1989, J. Bacteriol. 171: 3583-3585), or transduction (see, e.g., Burke et al., 2001, Proc. Natl. Acad. Sci. USA 98: 6289-6294). The introduction of DNA into a Pseudomonas cell may be effected by electroporation (see, e.g., Choi et al., 2006, J. Microbiol. Methods 64: 391-397) or conjugation (see, e.g., Pinedo and Smets, 2005, Appl. Environ. Microbiol. 71: 51-57). The introduction of DNA into a Streptococcus cell may be effected by natural competence (see, e.g., Perry and Kuramitsu, 1981, Infect. Immun. 32: 1295-1297), protoplast transformation (see, e.g., Catt and Jollick, 1991, Microbios 68: 189-207), electroporation (see, e.g., Buckley et al., 1999, Appl. Environ. Microbiol. 65: 3800-3804), or conjugation (see, e.g., Clewell, 1981, Microbiol. Rev. 45: 409-436). However, any method known in the art for introducing DNA into a host cell can be used.
(861) The host cell may also be a eukaryote, such as a mammalian, insect, plant, or fungal cell.
(862) The host cell may be a fungal cell. “Fungi” as used herein includes the phyla Ascomycota, Basidiomycota, Chytridiomycota, and Zygomycota as well as the Oomycota and all mitosporic fungi (as defined by Hawksworth et al., In, Ainsworth and Bisby's Dictionary of The Fungi, 8th edition, 1995, CAB International, University Press, Cambridge, UK).
(863) The fungal host cell may be a yeast cell. “Yeast” as used herein includes ascosporogenous yeast (Endomycetales), basidiosporogenous yeast, and yeast belonging to the Fungi Imperfecti (Blastomycetes). Since the classification of yeast may change in the future, yeast shall be defined as described in Biology and Activities of Yeast (Skinner, Passmore, and Davenport, editors, Soc. App. Bacteriol. Symposium Series No. 9, 1980).
(864) The yeast host cell may be a Candida, Hansenula, Kluyveromyces, Pichia, Saccharomyces, Schizosaccharomyces, or Yarrowia cell, such as a Kluyveromyces lactis, Saccharomyces carlsbergensis, Saccharomyces cerevisiae, Saccharomyces diastaticus, Saccharomyces douglasii, Saccharomyces kluyveri, Saccharomyces norbensis, Saccharomyces oviformis, or Yarrowia lipolytica cell.
(865) The fungal host cell may be a filamentous fungal cell. “Filamentous fungi” include all filamentous forms of the subdivision Eumycota and Oomycota (as defined by Hawksworth et al., 1995, supra). The filamentous fungi are generally characterized by a mycelial wall composed of chitin, cellulose, glucan, chitosan, mannan, and other complex polysaccharides. Vegetative growth is by hyphal elongation and carbon catabolism is obligately aerobic. In contrast, vegetative growth by yeasts such as Saccharomyces cerevisiae is by budding of a unicellular thallus and carbon catabolism may be fermentative.
(866) The filamentous fungal host cell may be an Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes, or Trichoderma cell.
(867) For example, the filamentous fungal host cell may be an Aspergillus awamori, Aspergillus foetidus, Aspergillus fumigatus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Bjerkandera adusta, Ceriporiopsis aneirina, Ceriporiopsis caregiea, Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsis rivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Chrysosporium inops, Chrysosporium keratinophilum, Chrysosporium lucknowense, Chrysosporium merdarium, Chrysosporium pannicola, Chrysosporium queenslandicum, Chrysosporium tropicum, Chrysosporium zonatum, Coprinus cinereus, Coriolus hirsutus, Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium sulphureum, Fusarium torulosum, Fusarium trichothecioides, Fusarium venenatum, Humicola insolens, Humicola lanuginosa, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Penicillium purpurogenum, Phanerochaete chrysosporium, Phlebia radiata, Pleurotus eryngii, Talaromyces emersonii, Thielavia terrestris, Trametes villosa, Trametes versicolor, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei, or Trichoderma viride cell.
(868) Fungal cells may be transformed by a process involving protoplast formation, transformation of the protoplasts, and regeneration of the cell wall in a manner known per se. Suitable procedures for transformation of Aspergillus and Trichoderma host cells are described in EP 238023, Yelton et al., 1984, Proc. Natl. Acad. Sci. USA 81: 1470-1474, and Christensen et al., 1988, Bio/Technology 6: 1419-1422. Suitable methods for transforming Fusarium species are described by Malardier et al., 1989, Gene 78: 147-156, and WO 96/00787. Yeast may be transformed using the procedures described by Becker and Guarente, In Abelson, J. N. and Simon, M. I., editors, Guide to Yeast Genetics and Molecular Biology, Methods in Enzymology, Volume 194, pp 182-187, Academic Press, Inc., New York; Ito et al., 1983, J. Bacteriol. 153: 163; and Hinnen et al., 1978, Proc. Natl. Acad. Sci. USA 75: 1920.
(869) Methods of Production
(870) The present disclosure also relates to methods of producing a dispersin variant, comprising (a) cultivating a cell, under conditions conducive for production of the dispersin variants; and optionally, (b) recovering the dispersin variant. In some aspects, the cell is an Terribacillus cell. In another aspect, the cell is a Terribacillus saccharophilus, Terribacillus goriensis or Terribacillus saccharophilus cell.
(871) Disclosed are also methods of producing a dispersin variant, comprising (a) cultivating a recombinant host cell under conditions conducive for production of the dispersin variant; and optionally, (b) recovering the dispersin variant.
(872) The host cells are cultivated in a nutrient medium suitable for production of the polypeptide using methods known in the art. For example, the cells may be cultivated by shake flask cultivation, or small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermenters in a suitable medium and under conditions allowing the polypeptide to be expressed and/or isolated. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection). If the polypeptide is secreted into the nutrient medium, the polypeptide can be recovered directly from the medium. If the polypeptide is not secreted, it can be recovered from cell lysates.
(873) The dispersin variant may be detected using methods known in the art that are specific for the dispersin variant polypeptide. These detection methods include, but are not limited to, use of specific antibodies, formation of an enzyme product, or disappearance of an enzyme substrate. For example, an enzyme assay may be used to determine the activity of the polypeptide.
(874) The dispersin variant polypeptide may be recovered using methods known in the art. For example, the dispersin variant polypeptide may be recovered from the nutrient medium by conventional procedures including, but not limited to, collection, centrifugation, filtration, extraction, spray-drying, evaporation, or precipitation. In some aspects, a fermentation broth comprising the dispersin variant is recovered.
(875) The polypeptide may be purified by a variety of procedures known in the art including, but not limited to, chromatography (e.g., ion exchange, affinity, hydrophobic, chromatofocusing, and size exclusion), electrophoretic procedures (e.g., preparative isoelectric focusing), differential solubility (e.g., ammonium sulfate precipitation), SDS-PAGE, or extraction (see, e.g., Protein Purification, Janson and Ryden, editors, VCH Publishers, New York, 1989) to obtain substantially pure polypeptides.
(876) In an alternative aspect, the polypeptide is not recovered, but rather a host cell expressing the polypeptide is used as a source of the variant.
(877) Cleaning Compositions
(878) Non-limiting embodiments are directed to cleaning e.g. detergent compositions comprising at least one dispersin variant as described herein, the above-defined additional components, and preferably a detergent adjunct ingredient. The detergent composition may be used for improving deep-cleaning effect, including but not limited to deep cleaning of an item, for preventing and/or reducing the stickiness of an item, for pretreating stains on the item, for preventing and/or reducing redeposition of soil during a wash cycle, for preventing and/or reducing adherence of soil to an item, for maintaining or improving the whiteness of an item and for preventing and/or reducing malodor from an item. The dispersin variants are useful in powder and liquid detergent.
(879) In some aspects, the detergent adjunct ingredient is selected from the group consisting of surfactants, builders, flocculating aid, chelating agents, dye transfer inhibitors, enzymes, enzyme stabilizers, enzyme inhibitors, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydrotropes, builders and co-builders, fabric hueing agents, anti-foaming agents, dispersants, processing aids, and/or pigments. These may be present in addition to the specified components defined above.
(880) The detergent adjunct ingredient may be a surfactant. One advantage of including a surfactant in a detergent composition comprising a dispersin variant is that the wash performance is improved. In some aspects, the detergent adjunct ingredient is a builder or a clay soil removal/anti-redeposition agent.
(881) In some aspects, detergent adjunct ingredient is an enzyme. The detergent composition may comprise one or more enzymes, as specified below. The one or more enzymes may be selected from the group consisting of proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases, nucleases e.g. DNases and RNases and mannanases. Specific enzymes suitable for the detergent compositions are described below.
(882) The cleaning composition may be formulated as a bar, a homogenous tablet, and a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a regular, compact or concentrated liquid. The detergent composition can be a liquid detergent, a powder detergent or a granule detergent.
(883) Some aspects relate to laundry or cleaning compositions comprising a dispersin, preferably at a level of from about 0.000001 wt % to about 1 wt %, from about 0.0001 wt % to about 1 wt %, from about 0.0002 wt % to about 1 wt %, from about 0.0005 wt % to about 1 wt %, from about 0.001 wt % to about 1 wt %, from about 0.002 wt % to about 1 wt %, from about 0.005 wt % to about 1 wt %, preferably from about 0.01 wt % to about 0.5 wt %, preferably from 0.0002 wt % to about 1 wt % by weight (wt %) of the composition. The amounts are wt % per unit active enzyme e.g. from about 0.00001 wt % to about 1 wt % of dispersin by weight of the composition.
(884) The concentration of the active enzyme having dispersin activity is preferably at least 0.00001%, preferably at least 0.00002%, preferably at least 0.0001 wt %, preferably at least 0.0002 wt %, preferably at least 0.001 wt %, preferably at least 0.002 wt %, preferably at least 0.005 wt %, preferably at least 0.01 wt %, preferably at least 0.02 wt %, preferably at least 0.05 wt % preferably at least 0.1 wt % of the total detergent concentration.
(885) The amount enzyme may also be in ppm (mg/L) active enzyme protein. Thus, in one aspect the amount of dispersin in the composition is at least 0.00001 ppm, 0.00002 ppm, 0.00005 ppm, 0.0001 ppm, 0.0002 ppm, 0.0005 ppm, 0.001 ppm, 0.002 ppm, 0.005 ppm, 0.01 ppm, 0.02 ppm, 0.05 ppm, 0.1 ppm, 0.2 ppm, 0.5 ppm, 1 ppm, 2 ppm, 5 ppm, 10 ppm or at least 20 ppm dispersin enzymes. In one aspect, the amount of dispersin in the composition is in the range from about 0.00001 ppm to about 10 ppm, or in the range from about 0.0001 ppm to about 2 ppm or in the range from about 0.001 ppm to about 2 ppm dispersin enzymes.
(886) In some aspects, the cleaning composition is a liquid or powder laundry detergent, suitable for e.g. washing at high temperature and/or pH, such as at or above 40° C. and/or at or above pH 8. In some aspects, the detergent composition is a liquid or powder laundry detergent, suitable for e.g. washing at low temperature and/or pH, such as at or below 20° C. and/or pH 6. The cleaning composition may also be formulated as a unit dose detergent and/or compact detergent optionally with minimum or no water. The cleaning composition may also be a dish wash detergent. The laundry and dish wash detergents may be phosphate-free.
(887) Surfactants
(888) The detergent composition may comprise one or more surfactants, which may be anionic and/or cationic and/or non-ionic and/or semi-polar and/or zwitterionic, or a mixture thereof. In a particular embodiment, the detergent composition includes a mixture of one or more nonionic surfactants and one or more anionic surfactants. The surfactant(s) is typically present at a level of from about 0.1% to 60% by weight, such as about 0.1% to about 15%, such as about 1% to about 40%, or about 3% to about 20%, or about 3% to about 10%. “About”, as used herein in relation to a numerical value means said value ±10%, preferably ±5%. “About 5 wt %” thus means from 4.5 to 5.5 wt %, preferably from 4.75 to 5.25 wt %. The surfactant(s) is chosen based on the desired cleaning application, and may include any conventional surfactant(s) known in the art. When included therein the detergent will usually contain from about 1% to about 40% by weight of an anionic surfactant, such as from about 5% to about 30%, including from about 5% to about 15%, or from about 15% to about 20%, or from about 20% to about 25% of an anionic surfactant. Non-limiting examples of anionic surfactants include sulfates and sulfonates, in particular, linear alkylbenzenesulfonates (LAS), isomers of LAS, branched alkylbenzenesulfonates (BABS), phenylalkanesulfonates, alpha-olefinsulfonates (AOS), olefin sulfonates, alkene sulfonates, alkane-2,3-diylbis(sulfates), hydroxyalkanesulfonates and disulfonates, alkyl sulfates (AS) such as sodium dodecyl sulfate (SDS), fatty alcohol sulfates (FAS), primary alcohol sulfates (PAS), alcohol ethersulfates (AES or AEOS or FES, also known as alcohol ethoxysulfates or fatty alcohol ether sulfates), secondary alkanesulfonates (SAS), paraffin sulfonates (PS), ester sulfonates, sulfonated fatty acid glycerol esters, alpha-sulfo fatty acid methyl esters (alpha-SFMe or SES) including methyl ester sulfonate (MES), alkyl- or alkenylsuccinic acid, dodecenyl/tetradecenyl succinic acid (DTSA), fatty acid derivatives of amino acids, diesters and monoesters of sulfo-succinic acid or salt of fatty acids (soap), and combinations thereof.
(889) When included therein the detergent will usually contain from about 1% to about 40% by weigh of a cationic surfactant, for example from about 0.5% to about 30%, in particular from about 1% to about 20%, from about 3% to about 10%, such as from about 3% to about 5%, from about 8% to about 12% or from about 10% to about 12%. Non-limiting examples of cationic surfactants include alkyldimethylethanolamine quat (ADMEAQ), cetyltrimethylammonium bromide (CTAB), dimethyldistearylammonium chloride (DSDMAC), and alkylbenzyldimethylammonium, alkyl quaternary ammonium compounds, alkoxylated quaternary ammonium (AQA) compounds, ester quats, and combinations thereof.
(890) When included therein the detergent will usually contain from about 0.2% to about 40% by weight of a nonionic surfactant, for example from about 0.5% to about 30%, in particular from about 1% to about 20%, from about 3% to about 10%, such as from about 3% to about 5%, from about 8% to about 12%, or from about 10% to about 12%. Non-limiting examples of nonionic surfactants include alcohol ethoxylates (AE or AEO), alcohol propoxylates, propoxylated fatty alcohols (PFA), alkoxylated fatty acid alkyl esters, such as ethoxylated and/or propoxylated fatty acid alkyl esters, alkylphenol ethoxylates (APE), nonylphenol ethoxylates (NPE), alkylpolyglycosides (APG), alkoxylated amines, fatty acid monoethanolamides (FAM), fatty acid diethanolamides (FADA), ethoxylated fatty acid monoethanolamides (EFAM), propoxylated fatty acid monoethanolamides (PFAM), polyhydroxyalkyl fatty acid amides, or N-acyl N-alkyl derivatives of glucosamine (glucamides, GA, or fatty acid glucamides, FAGA), as well as products available under the trade names SPAN and TWEEN, and combinations thereof. When included therein the detergent will usually contain from about 0.01% to about 10% by weight of a semipolar surfactant. Non-limiting examples of semipolar surfactants include amine oxides (AO) such as alkyldimethylamineoxide, N-(coco alkyl)-N,N-dimethylamine oxide and N-(tallow-alkyl)-N,N-bis(2-hydroxyethyl)amine oxide, and combinations thereof. When included therein the detergent will usually contain from about 0.01% to about 10% by weight of a zwitterionic surfactant. Non-limiting examples of zwitterionic surfactants include betaines such as alkyldimethylbetaines, sulfobetaines, and combinations thereof.
(891) Preferred anionic surfactants are sulphate surfactants and in particular alkyl ether sulphates, especially C9-C15 alcohol ether sulfates, preferably ethoxylates or mixed ethoxylates/propoxylates, such as those with 1 to 30 EO, C12-C15 primary alcohol ethoxylate, such as those with 1 to 30 EO, C8-C16 ester sulphates and C10-C14 ester sulphates, such as mono dodecyl ester sulphates. Non-limiting examples of anionic surfactants include sulfates and sulfonates, in particular, linear alkylbenzenesulfonates (LAS), in particular C12-C13 alkyl benzene sulfonates, isomers of LAS, branched alkylbenzenesulfonates (BABS), phenylalkanesulfonates, alpha-olefinsulfonates (AOS), olefin sulfonates, alkene sulfonates, alkane-2,3-diylbis(sulfates), hydroxyalkanesulfonates and disulfonates, alkyl sulfates (AS) such as sodium dodecyl sulfate (SDS), fatty alcohol sulfates (FAS), primary alcohol sulfates (PAS), alcohol ether sulfates (AES or AEOS or FES, also known as alcohol ethoxysulfates or fatty alcohol ether sulfates), secondary alkanesulfonates (SAS), paraffin sulfonates (PS), ester sulfonates, sulfonated fatty acid glycerol esters, alpha-sulfo fatty acid methyl esters (alpha-SFMe or SES) including methyl ester sulfonate (MES), alkyl- or alkenylsuccinic acid, dodecenyl/tetradecenyl succinic acid (DTSA), fatty acid derivatives of amino acids, diesters and monoesters of sulfo-succinic acid or salt of fatty acids (soap), and combinations thereof. The anionic surfactants are preferably added to the detergent in the form of salts. Suitable cations in these salts are alkali metal ions, such as sodium, potassium and lithium and ammonium salts, for example (2-hydroxyethyl) ammonium, bis(2-hydroxyethyl) ammonium and tris(2-hydroxyethyl) ammonium salts. Non-limiting examples of nonionic surfactants include alcohol ethoxylates (AE or AEO), alcohol propoxylates, propoxylated fatty alcohols (PFA), alkoxylated fatty acid alkyl esters, such as ethoxylated and/or propoxylated fatty acid alkyl esters, alkylphenol ethoxylates (APE), nonylphenol ethoxylates (NPE), alkylpolyglycosides (APG), alkoxylated amines, fatty acid monoethanolamides (FAM), fatty acid diethanolamides (FADA), ethoxylated fatty acid monoethanolamides (EFAM), propoxylated fatty acid monoethanolamides (PFAM), polyhydroxyalkyl fatty acid amides, or N-acyl N-alkyl derivatives of glucosamine (glucamides, GA, or fatty acid glucamides, FAGA), as well as products available under the trade names SPAN and TWEEN, and combinations thereof. Commercially available nonionic surfactants include Plurafac™, Lutensol™ and Pluronic™ range from BASF, Dehypon™ series from Cognis and Genapol™ series from Clariant.
(892) In various embodiments, said surfactant preferably comprises at least one alkyl ether sulfate. Preferred alkyl ether sulfates are those of formula (I)
R.sup.1—O-(AO).sub.n—SO.sub.3.sup.−X.sup.+ (I).
(893) In formula (I) R.sup.1 represents a linear or branched, substituted or unsubstituted alkyl group, preferably a linear, unsubstituted alkyl group, more preferably a fatty alcohol moiety. Preferred R.sup.1 moieties are selected from the group consisting of decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl moieties and mixtures thereof, wherein those groups with an even number of carbon atoms are preferred. Particularly preferred R.sup.1 moieties are derived from C.sub.10-C.sub.18 fatty alcohols, such as those derived from coconut oil alcohols, tallow fatty alcohols, lauryl, myristyl, cetyl or stearyl alcohol or from C.sub.10-C.sub.20 oxoalcohols.
(894) AO represents an ethyleneoxide (EO) or propyleneoxide (PO) group, preferably an ethyleneoxide group. The index n represents an integer from 1 to 50, preferably from 1 to 20 and more preferably from 1 to 10. Particularly preferably, n is 1, 2, 3, 4, 5, 6, 7 or 8. X represents a monovalent cation or the n-th part of an n-valent cation, preferred are alkali metal cations, specifically Na.sup.+ and K.sup.+, most preferably Na.sup.+. Further cations X.sup.+ may be selected from NH.sub.4.sup.+, ½ Zn.sup.2+, ½ Mg.sup.2+, ½ Ca.sup.2+, ½ Mn.sup.2+, and combinations thereof.
(895) In various preferred embodiments, the detergent compositions comprise an alkyl ether sulfate selected from fatty alcohol ether sulfates of formula (II)
(896) ##STR00001##
wherein k=9 to 19, and n=1, 2, 3, 4, 5, 6, 7 or 8. Preferred are C.sub.10-16 fatty alcohol ether sulfates with 1-7 EO (k=9-15, n=1-7), such as the C.sub.12-14 fatty alcohol ether sulfates with 1-3, particularly 2 EO (k=11-13, n=1-3 or 2), more particularly the sodium salts thereof. One specific embodiment thereof is lauryl ether sulfate sodium salt with 2 EO. The level of ethoxylation is an average value and can, for a specific compound, be an integer or fractional number.
(897) In various embodiments, the surfactant comprises at least one alkyl benzene sulfonate. Said alkyl benzene sulfonate may be present alternatively to the above alkyl ether sulfate or, preferably, in addition to it.
(898) Exemplary alkyl benzene sulfonates include, but are not limited to linear and branched alkyl benzene sulfonates, preferably linear alkyl benzene sulfonates. Exemplary compounds are those of formula (III)
(899) ##STR00002##
wherein R″ and R″ are independently H or alkyl and combined comprise 9 to 19, preferably 9 to 15 and more preferably 9 to 13 carbon atoms. Particularly preferred are dodecyl and tridecyl benzene sulfonates, in particular the sodium salts thereof.
(900) In addition or alternatively, the compositions may further comprise one or more nonionic surfactants. Preferred nonionic surfactants are those of formula (IV)
R.sup.2—O-(AO).sub.m—H (IV),
wherein R.sup.2 represents a linear or branched substituted or unsubstituted alkyl moiety, AO represents an ethylene oxide (EO) or propylene oxide (PO) group and m is an integer from 1 to 50.
(901) In formula (IV) R.sup.2 preferably represents a linear or branched, substituted or unsubstituted alkyl group, preferably a linear, unsubstituted alkyl group, particularly preferred a fatty alcohol group. Preferred groups are R.sup.2 are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl groups and combinations thereof, wherein those groups with an even number of carbon atoms are preferred. Particularly preferred are R.sup.2 groups derived from C.sub.12-C.sub.18 fatty alcohols, such as coconut oil alcohol, tallow oil alcohol, lauryl, myristyl, cetyl or stearyl alcohol or from C.sub.10-C.sub.20 oxoalcohols.
(902) AO represents an ethyleneoxide (EO) or propyleneoxide (PO) group, preferably an ethyleneoxide group. The index m represents an integer from 1 to 50, preferably from 1 to 20 and more preferably from 1 to 6. Particularly preferably, m is 1, 2, 3, 4 or 5, most preferably 3-5, as higher degrees of ethoxylation may negatively influence viscosity and stability.
(903) In various preferred embodiments, the detergent compositions comprise an alkyl ether selected from fatty alcohol ethers of formula (V)
(904) ##STR00003##
wherein k=11 to 19, m=1, 2, 3, 4, 5, 6, 7 or 8. Preferred are 012-18 fatty alcohols with 1-6 EO (k=11-17, m=1-5 in formula (V)). More preferred are C.sub.12-14 alcohols having 1-5 EO, most preferred are C.sub.12-14 alkyl ethers with 3-5 EO, in particular lauryl ether with 5 EO.
(905) The detergent compositions may further include other nonionic surfactants, such as alkyl glucosides of the general formula RO(G).sub.x, where R is a primary linear or 2-methyl-branched aliphatic radical containing 8 to 22 and preferably 12 to 18 carbon atoms and G stands for a glucose unit. The degree of oligomerization x, which indicates the distribution of monoglucosides and oligoglucosides, is a number of 1 to 10 and preferably a number of 1.2 to 1.4.
(906) In various embodiments, the composition comprises at least two anionic surfactants, e.g. at least one alkyl ether sulfate and preferably at least one alkyl benzene sulfonate, and optionally an alkyl ether.
(907) Suitable amphoteric surfactants comprise betains. Preferred betaines are the alkylbetaines, the alkylamidobetaines, the imidazolinium betaines, the sulfobetaines (INCI Sultaines) and the phosphobetaines. Examples of suitable betaines and sulfobetaines are the following compounds designated as INCI: almondamidopropyl betaines, apricotam idopropyl betaines, avocadamidopropyl betaines, babassuamidopropyl betaines, behenamide idopropyl betaines, behenyl betaines, betaines, canola idopropyl betaines, caprylic/capram idopropyl betaines, carnitines, cetyl betaines, Cocamidoethyl betaines, cocamidopropyl betaines, cocam idopropyl hydroxysultaines, cocobetaines, coco-hydroxysultaines, coco/oleam idopropyl betaines, coco-sultaines, decyl betaines, dihydroxyethyl oleyl glycinates, dihydroxyethyl soy glycinates, dihydroxyethyl stearyl glycinates, dihydroxyethyl tallow glycinates, dimethicones propyl PG Betaines, erucam idopropyl hydroxysultaines, hydrogenated tallow betaines, isostearam idopropyl betaines, lauram idopropyl betaines, lauryl betaines, lauryl hydroxysultaine, lauryl sultaines, milkamidopropyl betaines, minkam idopropyl betaines, myristamine idopropyl betaines, myristyl betaines, oleam idopropyl betaines, oleam idropy Hydroxysultain, Oleyl Betaine, Olivamidopropyl Betaine, Palmam Idopropyl Betaine, Palm Itam Idopropyl Betaine, Palmitoyl Carnitine, Palm Kernelamidopropyl Betaine, Polytetrafluoroethylene Acetoxypropyl Betaine, Ricinoleam Idopropyl Betaine, Sesamidopropyl Betaine, Soyamidopropyl Betaine, Stearam Idopropyl Betaine, Stearyl Betaine, Tallowam Idopropyl Betaine, Tallowamidopropyl Hydroxysultaine, Tallow Betaine, Tallow Dihydroxyethyl Betaine, Undecylenamidopropyl Betaine and Wheat Germamidopropyl Betaine. A preferred betaine is, for example, cocamidopropyl betaine (cocoamidopropylbetaine). The betaines are particularly preferred for dishwashing compositions, most preferably hand dishwashing detergent compositions.
(908) Further suitable surfactants include the amine oxides. The amine oxides suitable include alkylamine oxides, in particular alkyldimethylamine oxides, alkylamidoamine oxides and alkoxyalkylamine oxides. Examples of suitable amine oxides are the following compounds designated as INCI: Almond amidopropylamine oxides, Babassu amidopropylamine oxides, Behenamine oxides, Cocamidopropyl Amine oxides, Cocamidopropylamine oxides, Cocamine oxides, Coco-Morpholine oxides, Decylamine oxides, Decyltetradecylamine oxides, Diaminopyrimidine oxides, Dihydroxyethyl C8-10 alkoxypropylamines oxides, Dihydroxyethyl C9-11 alkoxypropylamines oxides, dihydroxyethyl C12-15 alkoxypropylamines oxides, dihydroxyethyl cocamine oxides, dihydroxyethyl lauramine oxides, dihydroxyethyl stearamines oxides, dihydroxyethyl tallowamine oxides, hydrogenated palm kernel amine oxides, hydrogenated tallowamine oxides, hydroxyethyl hydroxypropyl C12-15 alkoxypropylamines oxides, isostearamidopropylamines Oxides, isostearamidopropyl morpholine oxides, lauram idopropylamine oxides, lauramine oxides, methyl morpholine oxides, milkamidopropyl amine oxides, mincamidopropylamine oxides, myristamine idopropylamine oxides, myristamine oxides, myristyl/cetyl amines Oxides, Oleam idopropylamine oxides, Oleamine oxides, Ol ivam idopropylam ine oxides, Palmitamidopropylamine oxides, Palmitamine oxides, PEG-3 Lauramine oxides, Potassium dihydroxyethyl Cocamine oxides phosphates, Potassium Trisphosphonomethylamine oxides, Sesamidopropylamine oxides, Soyamidopropylamine oxides, Stearam idopropylam ine oxides, stearamines Oxides, Tallowam idopropylam ine oxides, Tallowamine oxides, Undecylenamidopropylamine oxides and Wheat Germam idopropylam ine oxides. A preferred amine oxide is, for example, cocamidopropylamine oxides (cocoamidopropylamine oxide).
(909) For automatic dishwashing applications, low-foaming nonionic surfactants are preferably used, in particular alkoxylated, especially ethoxylated, low-foaming nonionic surfactants. With particular preference, the automatic dishwashing detergents contain nonionic surfactants from the group of the alkoxylated alcohols. Particular preference is given to nonionic surfactants which have a melting point above room temperature. Nonionic surfactants having a melting point above 20° C., preferably above 25° C., more preferably between 25 and 60° C. and especially between 26.6 and 43.3° C., are particularly preferred. Preferably used surfactants are those from the groups of alkoxylated nonionic surfactants, in particular the ethoxylated primary alcohols and mixtures of these surfactants with structurally more complex surfactants such as polyoxypropylene/polyoxyethylene/polyoxypropylene ((PO/EO/PO) surfactants). Such (PO/EO/PO) nonionic surfactants are also characterized by good foam control. Particularly preferred nonionic surfactants are those containing alternating ethylene oxide and different alkylene oxide units. Among these, in turn, surfactants with EO-AO-EO-AO blocks are preferred, with one to ten EO or AO groups before one block from the other group follows. Exemplary nonionic surfactants are those having a C9-alkyl group with 1 to 4 ethylene oxide units followed by 1 to 4 propylene oxide units, followed by 1 to 4 ethylene oxide units followed by 1 to 4 propylene oxide units. Preference is given in particular to end-capped, poly (oxyalkylated) nonionic surfactants with the end-cap being a linear or branched, saturated or unsaturated, aliphatic or aromatic hydrocarbon radical R having 1 to 30 carbon atoms. The alkyl groups may also comprise hydroxyl groups. The group of these nonionic surfactants include, for example, the C4-22 fatty alcohol (EO).sub.10-50-2-hydroxyalkyl ethers, in particular also the C8-12 fatty alcohol (EO).sub.22-2-hydroxydecyl ethers and the C4-22 fatty alcohol (EO).sub.40-80-2-hydroxyalkyl ethers.
(910) Builders and Co-Builders
(911) The detergent composition may contain about 0-65% by weight, such as about 5% to about 50%, such as from about 0.5 to about 20% of a detergent builder or co-builder, or a mixture thereof. In a dish wash detergent, the level of builder is typically 40-65%, particularly 50-65%. The builder and/or co-builder may particularly be a chelating agent that forms water-soluble complexes with Ca and Mg. Any builder and/or co-builder known in the art for use in cleaning detergents may be utilized. Non-limiting examples of builders include zeolites, diphosphates (pyrophosphates), triphosphates such as sodium triphosphate (STP or STPP), carbonates such as sodium carbonate, soluble silicates such as sodium metasilicate, layered silicates (e.g., SKS-6 from Hoechst), ethanolamines such as 2-aminoethan-1-ol (MEA), diethanolamine (DEA, also known as 2,2′-iminodiethan-1-ol), triethanolamine (TEA, also known as 2,2′,2″-nitrilotriethan-1-ol), and (carboxymethyl)inulin (CMI), and combinations thereof.
(912) The detergent composition may also contain 0-50% by weight, such as about 5% to about 30%, of a detergent co-builder. The detergent composition may include a co-builder alone, or in combination with a builder, for example a zeolite builder. Non-limiting examples of co-builders include homopolymers of polyacrylates or copolymers thereof, such as poly(acrylic acid) (PAA) or copoly(acrylic acid/maleic acid) (PAA/PMA). Further non-limiting examples include citrate, chelators such as aminocarboxylates, aminopolycarboxylates and phosphonates, and alkyl- or alkenylsuccinic acid. Additional specific examples include 2,2′,2″-nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), iminodisuccinic acid (IDS), ethylenediamine-N,N′-disuccinic acid (EDDS), methylglycinediacetic acid (MGDA), glutamic acid-N,N-diacetic acid (GLDA), 1-hydroxyethane-1,1-diphosphonic acid (HEDP), ethylenediaminetetra(methylenephosphonic acid) (EDTMPA), diethylenetriaminepentakis(methylenephosphonic acid) (DTMPA or DTPMPA), N-(2-hydroxyethyl)iminodiacetic acid (EDG), aspartic acid-N-monoacetic acid (ASMA), aspartic acid-N,N-diacetic acid (ASDA), aspartic acid-N-monopropionic acid (ASMP), iminodisuccinic acid (IDA), N-(2-sulfomethyl)-aspartic acid (SMAS), N-(2-sulfoethyl)-aspartic acid (SEAS), N-(2-sulfomethyl)-glutamic acid (SMGL), N-(2-sulfoethyl)-glutamic acid (SEGO, N-methyliminodiacetic acid (MIDA), α-alanine-N,N-diacetic acid (α-ALDA), serine-N,N-diacetic acid (SEDA), isoserine-N,N-diacetic acid (ISDA), phenylalanine-N,N-diacetic acid (PHDA), anthranilic acid-N,N-diacetic acid (ANDA), sulfanilic acid-N,N-diacetic acid (SLDA), taurine-N,N-diacetic acid (TUDA) and sulfomethyl-N,N-diacetic acid (SMDA), N-(2-hydroxyethyl)ethylenediamine-N,N′,N″-triacetic acid (HEDTA), diethanolglycine (DEG), diethylenetriamine penta(methylenephosphonic acid) (DTPMP), aminotris(methylenephosphonic acid) (ATMP), and combinations and salts thereof. Further exemplary builders and/or co-builders are described in, e.g., WO 09/102854, U.S. Pat. No. 5,977,053
(913) Generally and if not indicated otherwise, the builder may be preferably selected from citrate, carbonate, silicate, aluminosilicate (zeolite) and combinations thereof. Suitable builders also include phosphonates, polyphosphonates, bicarbonates, borates, and further polycarboxylates. Citrate builders, e.g., citric acid and soluble salts thereof (particularly sodium salt), are particularly suitable water-soluble organic builders. Citrates can be used in combination with zeolite, silicates like the BRITESIL types, and/or layered silicate builders. The builder and/or co-builder may be any chelating agent that forms water-soluble complexes with Ca and Mg. Any builder and/or co-builder known in the art for use in cleaning detergents may be utilized. Non-limiting examples of builders include zeolites, in particular zeolite A or P or X, carbonates such as sodium carbonate, soluble silicates such as sodium metasilicate, layered silicates (e.g., SKS-6 from Hoechst), and (carboxymethyl)inulin (CMI), and combinations thereof. Further non-limiting examples of builders include aminocarboxylates, aminopolycarboxylates, and alkyl- or alkenylsuccinic acid. Additional specific examples include 2,2′,2″-nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), iminodisuccinic acid (IDS), ethylenediamine-N,N′-disuccinic acid (EDDS), methylglycine-N,N-diacetic acid (MGDA), glutamic acid-N,N-diacetic acid (GLDA), 1-hydroxyethane-1,1-diphosphonic acid, N-(2-hydroxyethyl)iminodiacetic acid (EDG), aspartic acid-N-monoacetic acid (ASMA), aspartic acid-N,N-diacetic acid (ASDA), aspartic acid-N-monopropionic acid (ASMP), iminodisuccinic acid (IDA), N-(sulfomethyl)aspartic acid (SMAS), N-(2-sulfoethyl)-aspartic acid (SEAS), N-(sulfomethylglutamic acid (SMGL), N-(2-sulfoethyl)-glutamic acid (SEGO, N-methyliminodiacetic acid (MIDA), serine-N,N-diacetic acid (SEDA), isoserine-N,N-diacetic acid (ISDA), phenylalanine-N,N-diacetic acid (PHDA), anthranilic acid-N,N-diacetic acid (ANDA), sulfanilic acid-N,N-diacetic acid (SLDA), taurine-N,N-diacetic acid (TUDA) and N″-(2-hydroxyethyhethylenediamine-N,N,N′-triacetic acid (HEDTA), diethanolglycine (DEG), and combinations and salts thereof. Phosphonates suitable for use herein include 1-hydroxyethane-1,1-diphosphonic acid (HEDP), ethylenediaminetetrakis (methylenephosphonic acid) (EDTMPA), diethylenetriaminepentakis (methylenephosphonic acid) (DTMPA or DTPMPA or DTPMP), nitrilotris (methylenephosphonic acid) (ATMP or NTMP), 2-phosphonobutane-1,2,4-tricarboxylic acid (PBTC), hexamethylenediaminetetrakis (methylenephosphonic acid) (HDTMP). Particularly preferred are HEDP and DTPMP.
(914) Suitable silicates are crystalline, layered sodium silicates of the general formula NaMSi.sub.xO.sub.2+1*yH.sub.2O, wherein M is sodium or H, x a number of from 1, 9 to 4 and y a number of from 0 to 20 and x is preferably 2, 3 or 4. Such silicates are for example disclosed in EP-A-0 164 514. Preferred are silicates in which M is sodium and is 2 or 3. Particularly preferred are β- and δ-sodium disilicate Na.sub.2Si.sub.2O.sub.5*yH.sub.2O.
(915) Although not preferred, the compositions may also comprise phosphates, diphosphates (pyrophosphates) and/or triphosphates such as sodium triphosphate (STP or STPP). It is however preferred that all compositions disclosed herein are phosphate-free, i.e. do not contain deliberately added phosphate, in particular the phosphate content is below 1 wt %, more preferably less than 0.5 wt %, even more preferably less than 0.1 wt %, relative to the total weight of the composition. In alternative embodiments, phosphate-free cleaning compositions in general that contain the polypeptides. In one aspect, the composition may be a phosphate-free cleaning composition comprising any one or more of the polypeptides having hexosaminidase activity disclosed herein.
(916) If not indicated otherwise, the composition may also contain 0-50% by weight, such as about 5% to about 30%, of a detergent co-builder. The composition may include a co-builder alone, or in combination with a builder, for example a zeolite builder. Non-limiting examples of co-builders include homopolymers of polyacrylates or copolymers thereof, such as poly (acrylic acid) (PAA) or copoly (acrylic acid/maleic acid) (PAA/PMA) or polyaspartic acid. Further exemplary builders and/or co-builders are described in, e.g., WO 09/102854, U.S. Pat. No. 5,977,053.
(917) Preferred as co-builders are acrylate-containing water-soluble polymers, such as alkali metal salts of polyacrylic acid or polymethacrylic acid, for example those having a molecular weight M.sub.w in the range of 600 to 750,000 g/mol, as determined by gel permeation chromatography (GPC) according to DIN 55672-1:2007-08 with THF as an eluent.
(918) Preferred polymers are polyacrylates with a molecular weight M.sub.w of 1,000 to 15,000 g/mol, more preferred, due to their solubility, are short-chain polyacrylates with a molecular weight M.sub.w of 1,000 to 10,000 g/mol, most preferred from 1,000 to 5,000 g/mol.
(919) Preferred acrylates are alkali metal salts of polymers of acrylic acid, preferably the sodium salts, in particular those with molecular weights in the range of 1,000 to 10,000 g/mol or 1,000 to 5,000 g/mol. Suitable acrylates are commercially available, for example under the tradename Acusol® from Dow Chemical. Suitable are also copolymers of acrylates, in particular those of acrylic acid and methacrylic acid, and acrylic acid or methacrylic acid and maleic acid.
(920) In preferred embodiments, the compositions comprise a sulfopolymer, preferably a copolymer comprising an ethylenically unsaturated sulfonate/sulfonic acid as a co-monomer. Particularly suitable are monomers of allyl sulfonic acids, such as allyloxybenzene sulfonic acid and methallyl sulfonic acid. Particularly preferred sulfonic acid group-containing monomers are 1-acrylamido propane sulfonic acid-1,2-acrylamido-2-propanesulfonic acid, 2-acrylamido-2-methyl-1-propanesulfonic acid, 2-methacrylamido-2-methyl-1-propanesulfonic acid. 3-methacrylamido-2-hydroxy-propanesulfonic acid, allylsulfonic acid, methallylsulfonic acid, allyloxybenzenesulfonic acid, methallyloxybenzolsulfonsaure, 2-hydroxy-3-(2-propenyloxy) propanesulfonic acid, 2-methyl-2-propenl-sulfonic acid, styrenesulfonic acid, vinylsulfonic acid, 3-sulfopropyl, 3-sulfa propyl, sulfomethacrylamide, sulfomethylmethacrylamide and mixtures of said acids or their water-soluble salts.
(921) The sulfopolymers are preferably copolymers of the afore-described monomers with unsaturated carboxylic acids, Especially preferred unsaturated carboxylic acids are acrylic acid, methacrylic acid, ethacrylic acid, chloroacrylic acid, alpha-cyanoacrylic acid, crotonic acid, alpha-phenyl-acrylic acid, maleic acid, maleic anhydride, fumaric acid, itaconic acid, citraconic acid, methylenemalonic acid, sorbic acid, cinnamic acid or mixtures thereof. Usable are of course also the unsaturated dicarboxylic acids. Preferred are copolymers with acrylates, in particular with acrylic acid and methacrylic acid, and acrylic acid or methacrylic acid and maleic acid.
(922) Such polymers are, for example, commercially available under the trade names Acusol®590 or Acusol® 588 from Dow Chemical.
(923) In one aspect, the cleaning compositions comprise a polypeptide as defined herein and at least one sulfopolymer, as defined above. Such compositions are preferably dishwashing compositions.
(924) In one preferred embodiment, the builder is a non-phosphorus based builder such as citric acid and/or methylglycine-N,N-diacetic acid (MGDA) and/or glutamic-N,N-diacetic acid (GLDA) and/or salts thereof.
(925) Bleach Components
(926) The detergent may contain 0-30% by weight, such as about 1% to about 20%, of a bleaching system. Any bleaching system comprising components known in the art for use in cleaning detergents may be utilized. Suitable bleaching system components include sources of hydrogen peroxide; sources of peracids; and bleach catalysts or boosters.
(927) Sources of hydrogen peroxide: Suitable sources of hydrogen peroxide are inorganic persalts, including alkali metal salts such as sodium percarbonate and sodium perborates (usually mono- or tetrahydrate), and hydrogen peroxide-urea (1/1).
(928) Sources of peracids: Peracids may be (a) incorporated directly as preformed peracids or (b) formed in situ in the wash liquor from hydrogen peroxide and a bleach activator (perhydrolysis) or (c) formed in situ in the wash liquor from hydrogen peroxide and a perhydrolase and a suitable substrate for the latter, e.g., an ester.
(929) a) Suitable preformed peracids include, but are not limited to, peroxycarboxylic acids such as peroxybenzoic acid and its ring-substituted derivatives, peroxy-α-naphthoic acid, peroxyphthalic acid, peroxylauric acid, peroxystearic acid, ε-phthalimidoperoxycaproic acid [phthalimidoperoxyhexanoic acid (PAP)], and o-carboxybenzamidoperoxycaproic acid; aliphatic and aromatic diperoxydicarboxylic acids such as diperoxydodecanedioic acid, diperoxyazelaic acid, diperoxysebacic acid, diperoxybrassylic acid, 2-decyldiperoxybutanedioic acid, and diperoxyphthalic, -isophthalic and -terephthalic acids; perimidic acids; peroxymonosulfuric acid; peroxydisulfuric acid; peroxyphosphoric acid; peroxysilicic acid; and mixtures of said compounds. It is understood that the peracids mentioned may in some cases be best added as suitable salts, such as alkali metal salts (e.g., Oxone®) or alkaline earth-metal salts.
b) Suitable bleach activators include those belonging to the class of esters, amides, imides, nitriles or anhydrides and, where applicable, salts thereof. Suitable examples are tetraacetylethylenediamine (TAED), sodium 4-[(3,5,5-trimethylhexanoyl)oxy]benzene-1-sulfonate (ISONOBS), sodium 4-(dodecanoyloxy)benzene-1-sulfonate (LOBS), sodium 4-(decanoyloxy)benzene-1-sulfonate, 4-(decanoyloxy)benzoic acid (DOBA), sodium 4-(nonanoyloxy)benzene-1-sulfonate (NOBS), and/or those disclosed in WO98/17767. A particular family of bleach activators of interest was disclosed in EP624154 and particularly preferred in that family is acetyl triethyl citrate (ATC). ATC or a short chain triglyceride like triacetin has the advantage that they are environmentally friendly. Furthermore, acetyl triethyl citrate and triacetin have good hydrolytical stability in the product upon storage and are efficient bleach activators. Finally ATC is multifunctional, as the citrate released in the perhydrolysis reaction may function as a builder.
(930) Bleach catalysts and boosters: The bleaching system may also include a bleach catalyst or booster. Some non-limiting examples of bleach catalysts that may be used in the compositions include manganese oxalate, manganese acetate, manganese-collagen, cobalt-amine catalysts and manganese triazacyclononane (MnTACN) catalysts; particularly preferred are complexes of manganese with 1,4,7-trimethyl-1,4,7-triazacyclononane (Me3-TACN) or 1,2,4,7-tetramethyl-1,4,7-triazacyclononane (Me4-TACN), in particular Me3-TACN, such as the dinuclear manganese complex [(Me3-TACN)Mn(O)3Mn(Me3-TACN)](PF6).sub.2, and [2,2′,2″-nitrilotris(ethane-1,2-diylazanylylidene-κN-methanylylidene)triphenolato-κ3O]manganese(III). The bleach catalysts may also be other metal compounds, such as iron or cobalt complexes.
(931) In some aspects, where a source of a peracid is included, an organic bleach catalyst or bleach booster may be used having one of the following formulae:
(932) ##STR00004##
(iii) and mixtures thereof; wherein each R1 is independently a branched alkyl group containing from 9 to 24 carbons or linear alkyl group containing from 11 to 24 carbons, preferably each R1 is independently a branched alkyl group containing from 9 to 18 carbons or linear alkyl group containing from 11 to 18 carbons, more preferably each R1 is independently selected from the group consisting of 2-propylheptyl, 2-butyloctyl, 2-pentylnonyl, 2-hexyldecyl, dodecyl, tetradecyl, hexadecyl, octadecyl, isononyl, isodecyl, isotridecyl and isopentadecyl.
(933) Other exemplary bleaching systems are described, e.g. in WO 2007/087258, WO 2007/087244, WO 2007/087259, EP 1867708 (Vitamin K) and WO 2007/087242. Suitable photobleaches may for example be sulfonated zinc or aluminium phthalocyanines.
(934) The choice of detergent components may include, for textile care, the consideration of the type of textile to be cleaned, the type and/or degree of soiling, the temperature at which cleaning is to take place, and the formulation of the detergent product. Although components mentioned below are categorized by general header according to a functionality, this is not to be construed as a limitation, as a component may comprise additional functionalities as will be appreciated by the skilled artisan, including the exemplary non-limiting components shown in below.
(935) Metal Care Agents
(936) Metal care agents may prevent or reduce the tarnishing, corrosion or oxidation of metals, including aluminium, stainless steel and non-ferrous metals, such as silver and copper. Suitable examples include one or more of the following:
(937) (a) benzatriazoles, including benzotriazole or bis-benzotriazole and substituted derivatives thereof. Benzotriazole derivatives are those compounds in which the available substitution sites on the aromatic ring are partially or completely substituted. Suitable substituents include linear or branch-chain Ci-C20-alkyl groups (e.g., C1-C20-alkyl groups) and hydroxyl, thio, phenyl or halogen such as fluorine, chlorine, bromine and iodine.
(b) metal salts and complexes chosen from the group consisting of zinc, manganese, titanium, zirconium, hafnium, vanadium, cobalt, gallium and cerium salts and/or complexes, the metals being in one of the oxidation states II, III, IV, V or VI. In one aspect, suitable metal salts and/or metal complexes may be chosen from the group consisting of Mn(II) sulphate, Mn(II) citrate, Mn(II) stearate, Mn(II) acetylacetonate, K{circumflex over ( )}TiF6 (e.g., K2TiF6), K{circumflex over ( )}ZrF6 (e.g., K2ZrF6), CoSO4, Co(NOs)2 and Ce(NOs)3, zinc salts, for example zinc sulphate, hydrozincite or zinc acetate;
(c) silicates, including sodium or potassium silicate, sodium disilicate, sodium metasilicate, crystalline phyllosilicate and mixtures thereof.
(938) Further suitable organic and inorganic redox-active substances that act as silver/copper corrosion inhibitors are disclosed in WO 94/26860 and WO 94/26859. Preferably the composition comprises from 0.1 to 5% by weight of the composition of a metal care agent, preferably the metal care agent is a zinc salt.
(939) Hydrotropes
(940) The detergent composition may contain 0-10% by weight, for example 0-5% by weight, such as about 0.5 to about 5%, or about 3% to about 5%, of a hydrotrope. Any hydrotrope known in the art for use in detergents may be utilized. Non-limiting examples of hydrotropes include sodium benzenesulfonate, sodium p-toluene sulfonate (STS), sodium xylene sulfonate (SXS), sodium cumene sulfonate (SCS), sodium cymene sulfonate, amine oxides, alcohols and polyglycolethers, sodium hydroxynaphthoate, sodium hydroxynaphthalene sulfonate, sodium ethylhexyl sulfate, and combinations thereof.
(941) Polymers
(942) The detergent composition may contain 0-10% by weight, such as 0.5-5%, 2-5%, 0.5-2% or 0.2-1% of a polymer. Any polymer known in the art for use in detergents may be utilized. The polymer may function as a co-builder as mentioned above, or may provide antiredeposition, fibre protection, soil release, dye transfer inhibition, grease cleaning and/or anti-foaming properties. Some polymers may have more than one of the above-mentioned properties and/or more than one of the below-mentioned motifs. Exemplary polymers include (carboxymethyl)cellulose (CMC), poly(vinyl alcohol) (PVA), poly(vinylpyrrolidone) (PVP), poly(ethyleneglycol) or poly(ethylene oxide) (PEG), ethoxylated poly(ethyleneimine), carboxymethyl inulin (CMI), and polycarboxylates such as PAA, PAA/PMA, poly-aspartic acid, and lauryl methacrylate/acrylic acid copolymers, hydrophobically modified CMC (HM-CMC) and silicones, copolymers of terephthalic acid and oligomeric glycols, copolymers of poly(ethylene terephthalate) and poly(oxyethene terephthalate) (PET-POET), PVP, poly(vinylimidazole) (PVI), poly(vinylpyridine-N-oxide) (PVPO or PVPNO) and polyvinylpyrrolidone-vinylimidazole (PVPVI). Further exemplary polymers include sulfonated polycarboxylates, polyethylene oxide and polypropylene oxide (PEO-PPO) and diquaternium ethoxy sulfate. Other exemplary polymers are disclosed in, e.g., WO 2006/130575. Salts of the above-mentioned polymers are also contemplated.
(943) Fabric Hueing Agents
(944) The detergent composition may also include fabric hueing agents such as dyes or pigments, which when formulated in detergent compositions can deposit onto a fabric when said fabric is contacted with a wash liquor comprising said detergent compositions and thus altering the tint of said fabric through absorption/reflection of visible light. Fluorescent whitening agents emit at least some visible light. In contrast, fabric hueing agents alter the tint of a surface as they absorb at least a portion of the visible light spectrum. Suitable fabric hueing agents include dyes and dye-clay conjugates, and may also include pigments. Suitable dyes include small molecule dyes and polymeric dyes. Suitable small molecule dyes include small molecule dyes selected from the group consisting of dyes falling into the Colour Index (C.I.) classifications of Direct Blue, Direct Red, Direct Violet, Acid Blue, Acid Red, Acid Violet, Basic Blue, Basic Violet and Basic Red, or mixtures thereof, for example as described in WO 2005/03274, WO 2005/03275, WO 2005/03276 and EP 1876226 (hereby incorporated by reference). The detergent composition preferably comprises from about 0.00003 wt % to about 0.2 wt %, from about 0.00008 wt % to about 0.05 wt %, or even from about 0.0001 wt % to about 0.04 wt % fabric hueing agent. The composition may comprise from 0.0001 wt % to 0.2 wt % fabric hueing agent, this may be especially preferred when the composition is in the form of a unit dose pouch. Suitable hueing agents are also disclosed in, e.g. WO 2007/087257 and WO 2007/087243.
(945) Enzymes
(946) The detergent composition may comprise one or more additional enzymes such as a protease, lipase, cutinase, amylase, carbohydrase, cellulase, pectinase, mannanase, arabinase, galactanase, xylanase, oxidase, e.g., a laccase, and/or peroxidase.
(947) In general, the properties of the selected enzyme(s) should be compatible with the selected detergent, (La, pH-optimum, compatibility with other enzymatic and non-enzymatic ingredients, etc.), and the enzyme(s) should be present in effective amounts.
(948) Cellulases
(949) Suitable cellulases include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Suitable cellulases include cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, Acremonium, e.g., the fungal cellulases produced from Humicola insolens, Myceliophthora thermophila and Fusarium oxysporum disclosed in U.S. Pat. Nos. 4,435,307, 5,648,263, 5,691,178, 5,776,757 and WO 89/09259.
(950) Especially suitable cellulases are the alkaline or neutral cellulases having colour care benefits. Examples of such cellulases are cellulases described in EP 0 495 257, EP 0 531 372, WO 96/11262, WO 96/29397, WO 98/08940. Other examples are cellulase variants such as those described in WO 94/07998, EP 0 531 315, U.S. Pat. Nos. 5,457,046, 5,686,593, 5,763,254, WO 95/24471, WO 98/12307 and WO 99/001544.
(951) Other cellulases are endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO: 2 of WO 2002/099091 or a family 44 xyloglucanase, which a xyloglucanase enzyme having a sequence of at least 60% identity to positions 40-559 of SEQ ID NO: 2 of WO 2001/062903.
(952) Commercially available cellulases include Celluzyme™, and Carezyme™ (Novozymes A/S) Carezyme Premium™ (Novozymes A/S), Celluclean™ (Novozymes A/S), Celluclean Classic™ (Novozymes A/S), Cellusoft™ (Novozymes A/S), Whitezyme™ (Novozymes A/S), Clazinase™, and Puradax HA™ (Genencor International Inc.), and KAC-500(B)™ (Kao Corporation).
(953) Proteases
(954) Suitable proteases include those of bacterial, fungal, plant, viral or animal origin e.g. vegetable or microbial origin. Microbial origin is preferred. Chemically modified or protein engineered mutants are included. It may be an alkaline protease, such as a serine protease or a neutral protease such as a metalloprotease. A serine protease may for example be of the S1 family, such as trypsin, or the S8 family such as subtilisin. A metalloproteases protease may for example be a thermolysin from e.g. family M4 or other metalloprotease such as those from M5, M7 or M8 families.
(955) The term “subtilases” refers to a sub-group of serine protease according to Siezen et al., Protein Engng. 4 (1991) 719-737 and Siezen et al. Protein Science 6 (1997) 501-523. Serine proteases are a subgroup of proteases characterized by having a serine in the active site, which forms a covalent adduct with the substrate. The subtilases may be divided into 6 sub-divisions, i.e. the Subtilisin family, the Thermitase family, the Proteinase K family, the Lantibiotic peptidase family, the Kexin family and the Pyrolysin family.
(956) Examples of subtilases are those derived from Bacillus such as Bacillus lentus, B. alkalophilus, B. subtilis, B. amyloliquefaciens, Bacillus pumilus and Bacillus gibsonii described in; U.S. Pat. No. 7,262,042 and WO09/021867, and subtilisin lentus, subtilisin novo, subtilisin Carlsberg, Bacillus licheniformis, subtilisin BPN′, subtilisin 309, subtilisin 147 and subtilisin 168 described in WO89/06279 and protease PD138 described in (WO93/18140). Other useful proteases may be those described in WO92/175177, WO01/016285, WO02/026024 and WO02/016547. Examples of trypsin-like proteases are trypsin (e.g. of porcine or bovine origin) and the Fusarium protease described in WO89/06270, WO94/25583 and WO05/040372, and the chymotrypsin proteases derived from Cellumonas described in WO05/052161 and WO05/052146.
(957) A further preferred protease is the alkaline protease from Bacillus lentus DSM 5483, as described for example in WO95/23221, and variants thereof which are described in WO92/21760, WO95/23221, EP1921147 and EP1921148.
(958) Examples of metalloproteases are the neutral metalloprotease as described in WO07/044993 (Genencor Int.) such as those derived from Bacillus amyloliquefaciens.
(959) Examples of useful proteases are the variants described in: WO92/19729, WO96/034946, WO98/20115, WO98/20116, WO99/011768, WO01/44452, WO03/006602, WO04/03186, WO04/041979, WO07/006305, WO11/036263, WO11/036264, especially the variants with substitutions in one or more of the following positions: 3, 4, 9, 15, 24, 27, 42, 55, 59, 60, 66, 74, 85, 96, 97, 98, 99, 100, 101, 102, 104, 116, 118, 121, 126, 127, 128, 154, 156, 157, 158, 161, 164, 176, 179, 182, 185, 188, 189, 193, 198, 199, 200, 203, 206, 211, 212, 216, 218, 226, 229, 230, 239, 246, 255, 256, 268 and 269 wherein the positions correspond to the positions of the Bacillus Lentus protease shown in SEQ ID NO: 1 of WO 2016/001449. More preferred the protease variants may comprise one or more of the following mutations: S3T, V41, S9R, S9E, A15T, S24G, S24R, K27R, N42R, S55P, G59E, G59D, N60D, N60E, V66A, N74D, S85R, A96S, S97G, S97D, S97A, S975D, S99E, S99D, S99G, S99M, S99N, S99R, S99H, S101A, V102I, V102Y, V102N, S104A, G116V, G116R, H118D, H118N, A120S, S126L, P127Q, S128A, S154D, A156E, G157D, G157P, S158E, Y161A, R164S, Q176E, N179E, S182E, Q185N, A188P, G189E, V193M, N198D, V199I, Y203W, S206G, L211Q, L211D, N212D, N212S, M216S, A226V, K229L, Q230H, Q239R, N246K, N255W, N255D, N255E, L256E, L256D T268A and R269H. The protease variants are preferably variants of the Bacillus lentus protease (Savinase®) shown in SEQ ID NO: 1 of WO 2016/001449 or the Bacillus amylolichenifaciens protease (BPN′) shown in SEQ ID NO: 2 of WO2016/001449. The protease variants preferably have at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1 or SEQ ID NO: 2 of WO 2016/001449.
(960) A protease variant comprising a substitution at one or more positions corresponding to positions 171, 173, 175, 179, or 180 of SEQ ID NO: 1 of WO2004/067737, wherein said protease variant has a sequence identity of at least 75% but less than 100% to SEQ ID NO: 1 of WO2004/067737.
(961) Suitable commercially available protease enzymes include those sold under the trade names Alcalase®, Duralase™, Durazym™, Relase®, Relase® Ultra, Savinase®, Savinase® Ultra, Primase®, Polarzyme®, Kannase®, Liquanase®, Liquanase® Ultra, Ovozyme®, Coronase®, Coronase® Ultra, Blaze®, Blaze Evity® 100T, Blaze Evity® 125T, Blaze Evity® 150T, Neutrase®, Everlase® and Esperase® (Novozymes A/S), those sold under the tradename Maxatase®, Maxacal®, Maxapem®, Purafect Ox®, Purafect OxP®, Puramax®, FN2®, FN3®, FN4®, Excellase®, Excellenz P1000™, Excellenz P1250™, Eraser®, Preferenz P100™, Purafect Prime®, Preferenz P110™, Effectenz P1000™, Purafect®™, Effectenz P1050™, Purafect Ox®™, Effectenz P2000™, Purafast®, Properase®, Opticlean® and Optimase® (Danisco/DuPont), Axapem™ (Gist-Brocases N.V.), BLAP (sequence shown in FIG. 29 of U.S. Pat. No. 5,352,604) and variants hereof (Henkel AG) and KAP (Bacillus alkalophilus subtilisin) from Kao.
(962) Lipases and Cutinases:
(963) Suitable lipases and cutinases include those of bacterial or fungal origin. Chemically modified or protein engineered mutant enzymes are included. Examples include lipase from Thermomyces, e.g. from T lanuginosus (previously named Humicola lanuginosa) as described in EP258068 and EP305216, cutinase from Humicola, e.g. H. insolens (WO96/13580), lipase from strains of Pseudomonas (some of these now renamed to Burkholderia), e.g. P. alcaligenes or P. pseudoalcaligenes (EP218272), P. cepacia (EP331376), P. sp. strain SD705 (WO95/06720 & WO96/27002), P. wisconsinensis (WO96/12012), GDSL-type Streptomyces lipases (WO10/065455), cutinase from Magnaporthe grisea (WO10/107560), cutinase from Pseudomonas mendocina (U.S. Pat. No. 5,389,536), lipase from Thermobifida fusca (WO11/084412), Geobacillus stearothermophilus lipase (WO11/084417), lipase from Bacillus subtilis (WO11/084599), and lipase from Streptomyces griseus (WO11/150157) and S. pristinaespiralis (WO12/137147).
(964) Other examples are lipase variants such as those described in EP407225, WO92/05249, WO94/01541, WO94/25578, WO95/14783, WO95/30744, WO95/35381, WO95/22615, WO96/00292, WO97/04079, WO97/07202, WO00/34450, WO00/60063, WO01/92502, WO07/87508 and WO09/109500.
(965) Preferred commercial lipase products include Lipolase™, Lipex™; Lipolex™ and Lipoclean™ (Novozymes A/S), Lumafast (originally from Genencor) and Lipomax (originally from Gist-Brocades).
(966) Still other examples are lipases sometimes referred to as acyltransferases or perhydrolases, e.g. acyltransferases with homology to Candida antarctica lipase A (WO10/111143), acyltransferase from Mycobacterium smegmatis (WO05/56782), perhydrolases from the CE 7 family (WO09/67279), and variants of the M. smegmatis perhydrolase in particular the S54V variant used in the commercial product Gentle Power Bleach from Huntsman Textile Effects Pte Ltd (WO10/100028).
(967) Amylases
(968) Suitable amylases which can be used together with the dispersins may be an alpha-amylase or a glucoamylase and may be of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Amylases include, for example, alpha-amylases obtained from Bacillus, e.g., a special strain of Bacillus licheniformis, described in more detail in GB 1,296,839.
(969) Suitable amylases include amylases having SEQ ID NO: 2 in WO 95/10603 or variants having 90% sequence identity to SEQ ID NO: 1 thereof. Preferred variants are described in WO 94/02597, WO 94/18314, WO 97/43424 and SEQ ID NO: 4 of WO 99/019467, such as variants with substitutions in one or more of the following positions: 15, 23, 105, 106, 124, 128, 133, 154, 156, 178, 179, 181, 188, 190, 197, 201, 202, 207, 208, 209, 211, 243, 264, 304, 305, 391, 408, and 444.
(970) Different suitable amylases include amylases having SEQ ID NO: 6 in WO 02/010355 or variants thereof having 90% sequence identity to SEQ ID NO: 6. Preferred variants of SEQ ID NO: 6 are those having a deletion in positions 181 and 182 and a substitution in position 193.
(971) Other amylases which are suitable are hybrid alpha-amylase comprising residues 1-33 of the alpha-amylase obtained from B. amyloliquefaciens shown in SEQ ID NO: 6 of WO 2006/066594 and residues 36-483 of the B. licheniformis alpha-amylase shown in SEQ ID NO: 4 of WO 2006/066594 or variants having 90% sequence identity thereof. Preferred variants of this hybrid alpha-amylase are those having a substitution, a deletion or an insertion in one of more of the following positions: G48, T49, G107, H156, A181, N190, M197, I201, A209 and Q264. Most preferred variants of the hybrid alpha-amylase comprising residues 1-33 of the alpha-amylase obtained from B. amyloliquefaciens shown in SEQ ID NO: 6 of WO 2006/066594 and residues 36-483 of SEQ ID NO: 4 are those having the substitutions: M197T; H156Y+A181T+N190F+A209V+Q264S; or G48A+T491+G107A+H156Y+A181T+N190F+I201F+A209V+Q264S.
(972) Further amylases which are suitable are amylases having SEQ ID NO: 6 in WO 99/019467 or variants thereof having 90% sequence identity to SEQ ID NO: 6. Preferred variants of SEQ ID NO: 6 are those having a substitution, a deletion or an insertion in one or more of the following positions: R181, G182, H183, G184, N195, 1206, E212, E216 and K269. Particularly preferred amylases are those having deletion in positions R181 and G182, or positions H183 and G184.
(973) Additional amylases which can be used are those having SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 2 or SEQ ID NO: 7 of WO 96/023873 or variants thereof having 90% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 7. Preferred variants of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 7 are those having a substitution, a deletion or an insertion in one or more of the following positions: 140, 181, 182, 183, 184, 195, 206, 212, 243, 260, 269, 304 and 476, using SEQ ID NO: 2 of WO 96/023873 for numbering. More preferred variants are those having a deletion in two positions selected from 181, 182, 183 and 184, such as 181 and 182, 182 and 183, or positions 183 and 184. Most preferred amylase variants of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 7 are those having a deletion in positions 183 and 184 and a substitution in one or more of positions 140, 195, 206, 243, 260, 304 and 476.
(974) Other amylases which can be used are amylases having SEQ ID NO: 2 of WO 08/153815, SEQ ID NO: 10 in WO 01/66712 or variants thereof having 90% sequence identity to SEQ ID NO: 2 of WO 08/153815 or 90% sequence identity to SEQ ID NO: 10 in WO 01/66712. Preferred variants of SEQ ID NO: 10 in WO 01/66712 are those having a substitution, a deletion or an insertion in one of more of the following positions: 176, 177, 178, 179, 190, 201, 207, 211 and 264.
(975) Further suitable amylases are amylases having SEQ ID NO: 2 of WO 09/061380 or variants having 90% sequence identity to SEQ ID NO: 2 thereof. Preferred variants of SEQ ID NO: 2 are those having a truncation of the C-terminus and/or a substitution, a deletion or an insertion in one of more of the following positions: Q87, Q98, S125, N128, T131, T165, K178, R180, S181, T182, G183, M201, F202, N225, S243, N272, N282, Y30S, R309, D319, Q320, Q359, K444 and G475. More preferred variants of SEQ ID NO: 2 are those having the substitution in one of more of the following positions: Q87E,R, Q98R, S125A, N128C, T131I, T165I, K178L, T182G, M201L, F202Y, N225E,R, N272E,R, S243Q,A,E,D, Y305R, R309A, Q320R, Q359E, K444E and G475K and/or deletion in position R180 and/or S181 or of T182 and/or G183. Most preferred amylase variants of SEQ ID NO: 2 are those having the substitutions:
(976) N128C+K178L+T182G+Y305R+G475K;
(977) N128C+K178L+T182G+F202Y+Y305R+D319T+G475K;
(978) S125A+N128C+K178L+T182G+Y305R+G475K; or
(979) S125A+N128C+T131I+T165I+K178L+T182G+Y305R+G475K wherein the variants are C-terminally truncated and optionally further comprises a substitution at position 243 and/or a deletion at position 180 and/or position 181.
(980) Other suitable amylases are the alpha-amylase having SEQ ID NO: 12 in WO 01/66712 or a variant having at least 90% sequence identity to SEQ ID NO: 12. Preferred amylase variants are those having a substitution, a deletion or an insertion in one of more of the following positions of SEQ ID NO: 12 in WO01/66712: R28, R118, N174; R181, G182, D183, G184, G186, W189, N195, M202, Y298, N299, K302, S303, N306, R310, N314; R320, H324, E345, Y396, R400, W439, R444, N445, K446, Q449, R458, N471, N484. Particular preferred amylases include variants having a deletion of D183 and G184 and having the substitutions R118K, N195F, R320K and R458K, and a variant additionally having substitutions in one or more position selected from the group: M9, G149, G182, G186, M202, T257, Y295, N299, M323, E345 and A339, most preferred a variant that additionally has substitutions in all these positions.
(981) Further suitable amylases are alpha-amylase variants comprising a modification in one or more positions corresponding to positions 1, 54, 56, 72, 109, 113, 116, 134, 140, 159, 167, 169, 172, 173, 174, 181, 182, 183, 184, 189, 194, 195, 206, 255, 260, 262, 265, 284, 289, 304, 305, 347, 391, 395, 439, 469, 444, 473, 476, or 477 of SEQ ID NO: 1, wherein the alpha-amylase variant has a sequence identity of at least 75% but less than 100% to SEQ ID NO: 13 or SEQ ID NO: 14 of WO 2016/180748.
(982) Other examples are amylase variants such as those described in WO 2011/098531, WO 2013/001078 and WO 2013/001087.
(983) Commercially available amylases are Duramyl™, Termamyl™, Fungamyl™, Stainzyme™, Stainzyme Plus™, Natalase™, Liquozyme X and BAN™ (from Novozymes A/S), and Rapidase™ Purastar™/Effectenz™, Powerase and Preferenz S100 (from Genencor International Inc./DuPont).
(984) Peroxidases/Oxidases
(985) A peroxidase is a peroxidase enzyme comprised by the enzyme classification EC 1.11.1.7, as set out by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUBMB), or any fragment obtained therefrom, exhibiting peroxidase activity.
(986) Suitable peroxidases include those of plant, bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Examples of useful peroxidases include peroxidases from Coprinopsis, e.g., from C. cinerea (EP 179,486), and variants thereof as those described in WO 93/24618, WO 95/10602, and WO 98/15257.
(987) A peroxidase also includes a haloperoxidase enzyme, such as chloroperoxidase, bromoperoxidase and compounds exhibiting chloroperoxidase or bromoperoxidase activity. Haloperoxidases are classified according to their specificity for halide ions. Chloroperoxidases (E.C. 1.11.1.10) catalyze formation of hypochlorite from chloride ions.
(988) In an aspect, the haloperoxidase is a chloroperoxidase. Preferably, the haloperoxidase is a vanadium haloperoxidase, i.e., a vanadate-containing haloperoxidase. In a preferred method the vanadate-containing haloperoxidase is combined with a source of chloride ion.
(989) Haloperoxidases have been isolated from many different fungi, in particular from the fungus group dematiaceous hyphomycetes, such as Caldariomyces, e.g., C. fumago, Alternaria, Curvularia, e.g., C. verruculosa and C. inaequalis, Drechslera, Ulocladium and Botrytis.
(990) Haloperoxidases have also been isolated from bacteria such as Pseudomonas, e.g., P. pyrrocinia and Streptomyces, e.g., S. aureofaciens.
(991) In a preferred aspect, the haloperoxidase is derivable from Curvularia sp., in particular Curvularia verruculosa or Curvularia inaequalis, such as C. inaequalis CBS 102.42 as described in WO 95/27046; or C. verruculosa CBS 147.63 or C. verruculosa CBS 444.70 as described in WO 97/04102; or from Drechslera hartlebii as described in WO 01/79459, Dendryphiella salina as described in WO 01/79458, Phaeotrichoconis crotalarie as described in WO 01/79461, or Geniculosporium sp. as described in WO 01/79460.
(992) An oxidase include, in particular, any laccase enzyme comprised by the enzyme classification EC 1.10.3.2, or any fragment obtained therefrom exhibiting laccase activity, or a compound exhibiting a similar activity, such as a catechol oxidase (EC 1.10.3.1), an o-aminophenol oxidase (EC 1.10.3.4), or a bilirubin oxidase (EC 1.3.3.5).
(993) Preferred laccase enzymes are enzymes of microbial origin. The enzymes may be obtained from plants, bacteria or fungi (including filamentous fungi and yeasts).
(994) Suitable examples from fungi include a laccase derivable from a strain of Bacillus, Neurospora, e.g., N. crassa, Podospora, Botrytis, Collybia, Fomes, Lentinus, Pleurotus, Trametes, e.g., T. villosa and T. versicolor, Rhizoctonia, e.g., R. solani, Coprinopsis, e.g., C. cinerea, C. comatus, C. friesii, and C. plicatilis, Psathyrella, e.g., P. condelleana, Panaeolus, e.g., P. papilionaceus, Myceliophthora, e.g., M. thermophila, Schytalidium, e.g., S. thermophilum, Polyporus, e.g., P. pinsitus, Phlebia, e.g., P. radiata (WO 92/01046), or Coriolus, e.g., C. hirsutus (JP 2238885).
(995) Suitable examples from bacteria include a laccase derivable from a strain of Bacillus.
(996) A laccase obtained from Coprinopsis or Myceliophthora is preferred; in particular a laccase obtained from Coprinopsis cinerea, as disclosed in WO 97/08325; or from Myceliophthora thermophila, as disclosed in WO 95/33836.
(997) The detergent enzyme(s) may be included in a detergent composition by adding separate additives containing one or more enzymes, or by adding a combined additive comprising all of these enzymes. A detergent additive, i.e., a separate additive or a combined additive, can be formulated, for example, as a granulate, liquid, slurry, etc. Preferred detergent additive formulations are granulates, in particular non-dusting granulates, liquids, in particular stabilized liquids, or slurries.
(998) Non-dusting granulates may be produced, e.g. as disclosed in U.S. Pat. Nos. 4,106,991 and 4,661,452 and may optionally be coated by methods known in the art. Examples of waxy coating materials are poly (ethylene oxide) products (polyethyleneglycol, PEG) with mean molar weights of 1000 to 20000; ethoxylated nonylphenols having from 16 to 50 ethylene oxide units; ethoxylated fatty alcohols in which the alcohol contains from 12 to 20 carbon atoms and in which there are 15 to 80 ethylene oxide units; fatty alcohols; fatty acids; and mono- and di- and triglycerides of fatty acids. Examples of film-forming coating materials suitable for application by fluid bed techniques are given in GB 1483591. Liquid enzyme preparations may, for instance, be stabilized by adding a polyol such as propylene glycol, a sugar or sugar alcohol, lactic acid or boric acid according to established methods. Protected enzymes may be prepared according to the method disclosed in EP 238,216.
(999) Dispersants
(1000) The detergent compositions can also contain dispersants. In particular powdered detergents may comprise dispersants. Suitable water-soluble organic materials include the homo- or co-polymeric acids or their salts, in which the polycarboxylic acid comprises at least two carboxyl radicals separated from each other by not more than two carbon atoms. Suitable dispersants are for example described in Powdered Detergents, Surfactant science series volume 71, Marcel Dekker, Inc.
(1001) Dye Transfer Inhibiting Agents
(1002) The cleaning compositions may also include one or more dye transfer inhibiting agents. Suitable polymeric dye transfer inhibiting agents include, but are not limited to, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidones and polyvinylimidazoles or mixtures thereof. When present in a subject composition, the dye transfer inhibiting agents may be present at levels from about 0.0001% to about 10%, from about 0.01% to about 5% or even from about 0.1% to about 3% by weight of the composition.
(1003) Fluorescent Whitening Agent
(1004) The detergent composition may preferably also contain additional components that may tint articles being cleaned, such as fluorescent whitening agent or optical brighteners. Where present the brightener is preferably at a level of about 0.01% to about 0.5%. Any fluorescent whitening agent suitable for use in a laundry detergent composition may be used in the composition. The most commonly used fluorescent whitening agents are those belonging to the classes of diaminostilbene-sulfonic acid derivatives, diarylpyrazoline derivatives and bisphenyl-distyryl derivatives. Examples of the diaminostilbene-sulfonic acid derivative type of fluorescent whitening agents include the sodium salts of: 4,4′-bis-(2-diethanolamino-4-anilino-s-triazin-6-ylamino) stilbene-2,2′-disulfonate, 4,4′-bis-(2,4-dianilino-s-triazin-6-ylamino) stilbene-2.2′-disulfonate, 4,4′-bis-(2-anilino-4-(N-methyl-N-2-hydroxy-ethylamino)-s-triazin-6-ylamino) stilbene-2,2′-disulfonate, 4,4′-bis-(4-phenyl-1,2,3-triazol-2-yl)stilbene-2,2′-disulfonate and sodium 5-(2H-naphtho[1,2-d][1,2,3]triazol-2-yl)-2-[(E)-2-phenylvinyl]benzenesulfonate. Preferred fluorescent whitening agents are Tinopal DMS and Tinopal CBS available from Ciba-Geigy AG, Basel, Switzerland. Tinopal DMS is the disodium salt of 4,4′-bis-(2-morpholino-4-anilino-s-triazin-6-ylamino) stilbene-2,2′-disulfonate. Tinopal CBS is the disodium salt of 2,2′-bis-(phenyl-styryl)-disulfonate. Also preferred are fluorescent whitening agents is the commercially available Parawhite KX, supplied by Paramount Minerals and Chemicals, Mumbai, India. Tinopal CBS-X is a 4.4′-bis-(sulfostyryl)-biphenyl disodium salt also known as Disodium Distyrylbiphenyl Disulfonate. Other fluorescers suitable include the 1-3-diaryl pyrazolines and the 7-alkylaminocoumarins.
(1005) Suitable fluorescent brightener levels include lower levels of from about 0.01, from 0.05, from about 0.1 or even from about 0.2 wt % to upper levels of 0.5 or even 0.75 wt %.
(1006) Soil Release Polymers
(1007) The detergent compositions may also include one or more soil release polymers which aid the removal of soils from fabrics such as cotton and polyester based fabrics, in particular the removal of hydrophobic soils from polyester based fabrics. The soil release polymers may for example be nonionic or anionic terephthalate based polymers, polyvinyl caprolactam and related copolymers, vinyl graft copolymers, polyester polyamides see for example Chapter 7 in Powdered Detergents, Surfactant science series volume 71, Marcel Dekker, Inc. Another type of soil release polymers are amphiphilic alkoxylated grease cleaning polymers comprising a core structure and a plurality of alkoxylate groups attached to that core structure. The core structure may comprise a polyalkylenimine structure or a polyalkanolamine structure as described in detail in WO 2009/087523 (hereby incorporated by reference). Furthermore, random graft co-polymers are suitable soil release polymers. Suitable graft co-polymers are described in more detail in WO 2007/138054, WO 2006/108856 and WO 2006/113314 (hereby incorporated by reference). Other soil release polymers are substituted polysaccharide structures especially substituted cellulosic structures such as modified cellulose derivatives such as those described in EP 1867808 or WO 2003/040279 (both are hereby incorporated by reference). Suitable cellulosic polymers include cellulose, cellulose ethers, cellulose esters, cellulose amides and mixtures thereof. Suitable cellulosic polymers include anionically modified cellulose, nonionically modified cellulose, cationically modified cellulose, zwitterionically modified cellulose, and mixtures thereof. Suitable cellulosic polymers include methyl cellulose, carboxy methyl cellulose, ethyl cellulose, hydroxyl ethyl cellulose, hydroxyl propyl methyl cellulose, ester carboxy methyl cellulose, and mixtures thereof.
(1008) Anti-Redeposition Agents
(1009) The detergent compositions may also include one or more anti-redeposition agents such as carboxymethylcellulose (CMC), polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), polyoxyethylene and/or polyethyleneglycol (PEG), homopolymers of acrylic acid, copolymers of acrylic acid and maleic acid, and ethoxylated polyethyleneimines. The cellulose based polymers described under soil release polymers above may also function as anti-redeposition agents.
(1010) Rheology Modifiers
(1011) The detergent compositions may also include one or more rheology modifiers, structurants or thickeners, as distinct from viscosity reducing agents. The rheology modifiers are selected from the group consisting of non-polymeric crystalline, hydroxy-functional materials, polymeric rheology modifiers which impart shear thinning characteristics to the aqueous liquid matrix of a liquid detergent composition. The rheology and viscosity of the detergent can be modified and adjusted by methods known in the art, for example as shown in EP 2169040.
(1012) Other suitable adjunct materials include, but are not limited to, anti-shrink agents, anti-wrinkling agents, bactericides, binders, carriers, dyes, enzyme stabilizers, fabric softeners, fillers, foam regulators, hydrotropes, perfumes, pigments, sod suppressors, solvents, and structurants for liquid detergents and/or structure elasticizing agents.
(1013) Other Materials
(1014) Any detergent components known in the art for use in the cleaning composition may also be utilized. Other optional detergent components include anti-corrosion agents, anti-shrink agents, anti-soil redeposition agents, anti-wrinkling agents, bactericides, binders, corrosion inhibitors, disintegrants/disintegration agents, dyes, enzyme stabilizers (including boric acid, borates, CMC, and/or polyols such as propylene glycol), fabric conditioners including clays, fillers/processing aids, fluorescent whitening agents/optical brighteners, foam boosters, foam (suds) regulators, perfumes, soil-suspending agents, softeners, suds suppressors, tarnish inhibitors, and wicking agents, either alone or in combination. Any ingredient known in the art for use in detergents may be utilized. The choice of such ingredients is well within the skill of the artisan.
(1015) Formulation of Detergent Products
(1016) The detergent composition may be in any convenient form, e.g., a bar, a homogenous tablet, a tablet having two or more layers, a regular or compact powder, a granule, a paste, a gel, or a regular, compact or concentrated liquid.
(1017) Detergent formulation forms: Layers (same or different phases), Pouches, versus forms for Machine dosing unit.
(1018) Pouches can be configured as single or multicompartments. It can be of any form, shape and material which is suitable for hold the composition, e.g. without allowing the release of the composition to release of the composition from the pouch prior to water contact. The pouch is made from water soluble film which encloses an inner volume. Said inner volume can be divided into compartments of the pouch. Preferred films are polymeric materials preferably polymers which are formed into a film or sheet. Preferred polymers, copolymers or derivates thereof are selected polyacrylates, and water soluble acrylate copolymers, methyl cellulose, carboxy methyl cellulose, sodium dextrin, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, malto dextrin, poly methacrylates, most preferably polyvinyl alcohol copolymers and, hydroxyprpyl methyl cellulose (HPMC). Preferably the level of polymer in the film for example PVA is at least about 60%. Preferred average molecular weight will typically be about 20,000 to about 150,000. Films can also be of blend compositions comprising hydrolytically degradable and water soluble polymer blends such as polylactide and polyvinyl alcohol (known under the Trade reference M8630 as sold by Chris Craft In. Prod. Of Gary, Ind., US) plus plasticisers like glycerol, ethylene glycerol, Propylene glycol, sorbitol and mixtures thereof. The pouches can comprise a solid laundry cleaning composition or part components and/or a liquid cleaning composition or part components separated by the water soluble film. The compartment for liquid components can be different in composition than compartments containing solids. Ref: (US 2009/0011970 A1)
(1019) Detergent ingredients can be separated physically from each other by compartments in water dissolvable pouches or in different layers of tablets. Thereby negative storage interaction between components can be avoided. Different dissolution profiles of each of the compartments can also give rise to delayed dissolution of selected components in the wash solution.
(1020) Definition/Characteristics of the Forms:
(1021) A liquid or gel detergent, which is not unit dosed, may be aqueous, typically containing at least 20% by weight and up to 95% water, such as up to about 70% water, up to about 65% water, up to about 55% water, up to about 45% water, up to about 35% water. Other types of liquids, including without limitation, alkanols, amines, diols, ethers and polyols may be included in an aqueous liquid or gel. An aqueous liquid or gel detergent may contain from 0-30% organic solvent.
(1022) A liquid or gel detergent may be non-aqueous.
(1023) Granular Detergent Formulations
(1024) A granular detergent may be formulated as described in WO 09/092699, EP 1705241, EP 1382668, WO 07/001262, U.S. Pat. No. 6,472,364, WO 04/074419 or WO 09/102854. Other useful detergent formulations are described in WO 09/124162, WO 09/124163, WO 09/117340, WO 09/117341, WO 09/117342, WO 09/072069, WO 09/063355, WO 09/132870, WO 09/121757, WO 09/112296, WO 09/112298, WO 09/103822, WO 09/087033, WO 09/050026, WO 09/047125, WO 09/047126, WO 09/047127, WO 09/047128, WO 09/021784, WO 09/010375, WO 09/000605, WO 09/122125, WO 09/095645, WO 09/040544, WO 09/040545, WO 09/024780, WO 09/004295, WO 09/004294, WO 09/121725, WO 09/115391, WO 09/115392, WO 09/074398, WO 09/074403, WO 09/068501, WO 09/065770, WO 09/021813, WO 09/030632, and WO 09/015951.
(1025) Other useful detergent formulations are described in WO 2011025615, WO 2011016958, WO 2011005803, WO 2011005623, WO 2011005730, WO 2011005844, WO 2011005904, WO 2011005630, WO 2011005830, WO 2011005912, WO 2011005905, WO 2011005910, WO 2011005813, WO 2010135238, WO 2010120863, WO 2010108002, WO 2010111365, WO 2010108000, WO 2010107635, WO 2010090915, WO 2010033976, WO 2010033746, WO 2010033747, WO 2010033897, WO 2010033979, WO 2010030540, WO 2010030541, WO 2010030539, WO 2010024467, WO 2010024469, WO 2010024470, WO 2010025161, WO 2010014395, WO 2010044905,
(1026) Other useful detergent formulations are described in WO 2010145887, WO 2010142503, WO 2010122051, WO 2010102861, WO 2010099997, WO 2010084039, WO 2010076292, WO 2010069742, WO 2010069718, WO 2010069957, WO 2010057784, WO 2010054986, WO 2010018043, WO 2010003783, WO 2010003792,
(1027) Other useful detergent formulations are described in WO 2011023716, WO 2010142539, WO 2010118959, WO 2010115813, WO 2010105942, WO 2010105961, WO 2010105962, WO 2010094356, WO 2010084203, WO 2010078979, WO 2010072456, WO 2010069905, WO 2010076165, WO 2010072603, WO 2010066486, WO 2010066631, WO 2010066632, WO 2010063689, WO 2010060821, WO 2010049187, WO 2010031607, WO 2010000636.
(1028) Formulation of Enzyme in Co-Granule
(1029) The dispersin may be formulated as a granule for example as a co-granule that combines one or more enzymes. Each enzyme will then be present in more granules securing a more uniform distribution of enzymes in the detergent. This also reduces the physical segregation of different enzymes due to different particle sizes. Methods for producing multi-enzyme co-granulate for the detergent industry is disclosed in the IP.com disclosure IPCOM000200739D.
(1030) Another example of formulation of enzymes by the use of co-granulates are disclosed in WO 2013/188331, which relates to a detergent composition comprising (a) a multi-enzyme co-granule; (b) less than 10 wt zeolite (anhydrous basis); and (c) less than 10 wt phosphate salt (anhydrous basis), wherein said enzyme co-granule comprises from 10 to 98 wt % moisture sink components and the composition additionally comprises from 20 to 80 wt % detergent moisture sink components.
(1031) WO 2013/188331 also relates to a method of treating and/or cleaning a surface, preferably a fabric surface comprising the steps of (i) contacting said surface with the detergent composition as claimed and described herein aqueous wash liquor, (ii) rinsing and/or drying the surface.
(1032) Methods
(1033) Assays and Detergent Compositions
(1034) Composition of Model Detergent A (Liquid)
(1035) Ingredients: 12% LAS, 11% AEO Biosoft N25-7 (NI), 7% AEOS (SLES), 6% MPG (monopropylene glycol), 3% ethanol, 3% TEA, 2.75% cocoa soap, 2.75% soya soap, 2% glycerol, 2% sodium hydroxide, 2% sodium citrate, 1% sodium formiate, 0.2% DTMPA and 0.2% PCA (all percentages are w/w (weigth percent %))
(1036) Unless otherwise mentioned the tested variants were made from a site-saturation library as described below.
(1037) Construction of SSL
(1038) A site-saturation library was constructed for each amino acid position in the enzyme. The resulting transformants were plated onto LB agar plates containing with 6 μg/ml chloramphenicol and grown overnight at 37° C. The following day 188 individual colonies were picked from each SSL and used to inoculate 200 μl TB-Gly with 6 μg/ml chloramphenicol in 96 well MTPs. The plates were grown overnight and the mutation contained in each clone was identified by sequencing. A culture representing each of the 20 possible amino acid substitutions at each position in the enzyme was then transferred from the grown MTPs to a new MTP and all these collected variants were for screening.
(1039) Growth and Expression
(1040) Cultures inoculated from the MTPs of collected variants were grown in deep well plates with 600 μl Cal-18 (Ostergaard P R, Wilting R, Lassen S F. 2010. Identification and characterization of a bacterial glutamic peptidase. BMC Biochem. 11:47) supplemented with 6 μg/ml chloramphenicol at 37° C. for one day with shaking at 700 rpm. The wild type was also inoculated as reference in four wells on each microtiterplate. After growth, the plates were centrifuged to pellet the cells and the variants present in the supernatants were screened for improved stability.
EXAMPLES
Example 1 Growth and Expression
(1041) The constructed variants were plated on LB agar supplemented with 6 μg/ml chloramphenicol and grown for 37° C. for one day. After growth, colonies were picked to individual wells of deep well 96-well microtiter plates or standard 96-well microtiter plates containing 600 μl or 200 ul Ca118-2 (Ostergaard P R, Wilting R, Lassen S F. 2010. Identification and characterization of a bacterial glutamic peptidase. BMC Biochem. 11:47) broth, respectively, supplemented with 6 μg/ml chloramphenicol. The wild type dispersin (SEQ ID NO 1), was also inoculated as reference in four wells on each microtiterplate. The deepwell microtiter plates were grown for one day at 37° C. with shaking at 700 rpm and the standard 96-well microtiter plates grown for 3 days at 30° C. with shaking at 225 rpm.
Example 2a Testing the Variants for Stability
(1042) Measurement of Thermostability at pH 8
(1043) The variants were made as a site-saturation library as described above. Each supernatant sample, grown as stated in example 1, was diluted 20-fold in 50 mM Tris-HCl, pH 8.0. The sample was split in two parts: One part was incubated for 60 minutes at room temperature (21° C., unstressed sample) whereas the second part was incubated in a PCR machine for 60 minutes at 41° C. (stressed sample). After the incubation, 5 μl of the samples was transferred to the 384-well MTP containing 35 μl dispersin assay solution (45 mM citrate buffer pH 5 added 0.5 mg/ml p-nitrophenyl-N-acetyl-β-D-glucosaminide). The 384-well plate was then incubated at room temperature for 3 hours. After incubation, 40 μl stop solution (0.4 M Na.sub.2CO.sub.3) was added to the sample and absorbance at 405 nm was read. The measurements were corrected for the background by subtraction of the absorbance measurement obtained for a reference without dispersin. The residual activity (RA) for each dispersin variant and the reference dispersin was calculated as the ratio of background corrected activity between a stressed and an unstressed sample. These values were subsequently used to calculate the residual activity ratio (RAR=RA.sub.variant/RA.sub.reference), which was used as selection criteria of hits with improved stability. Variants with improved stability will have a RAR>1 as the RAR of the backbone in this setup will by definition be 1.0. The result is shown in Table 1.
(1044) TABLE-US-00002 TABLE 1 variant RAR variant RAR variant RAR variant RAR D2V 1.23 Y124K 1.22 S199L 1.20 A284T 3.32 S12A 2.58 D125R 3.36 A203G 3.06 A284L 3.01 T17E 2.53 D125C 2.35 A203E 1.36 A284I 1.47 V18L 1.22 D125G 2.01 A203V 1.10 S288K 4.43 K22M 2.17 D125H 1.58 N204L 2.02 V290I 1.43 S23I 1.65 D125K 1.29 N204Y 1.72 K291R 1.36 S23C 1.45 D125Q 1.18 N204V 1.21 T296C 1.18 S23A 1.45 Q135M 1.16 L205I 1.78 T303Q 4.45 S23T 1.41 D138R 2.44 D207K 3.47 D304M 2.00 S23L 1.33 D138K 1.57 D207S 3.01 D304C 1.12 S23V 1.24 D138N 1.32 D207C 2.06 L305M 4.54 S23E 1.18 D138Q 1.14 D207G 1.90 L305N 1.19 L24I 2.68 S163P 1.67 S210T 1.32 S306C 1.48 V25R 1.31 L170D 2.47 Q215K 3.95 K308D 6.09 Y30A 3.11 L170K 1.78 Q215R 2.59 K308E 5.11 Y30L 1.85 L170S 1.43 S221N 1.22 K308A 3.83 A49W 2.38 L170H 1.33 N227T 1.99 K308V 3.79 A49Y 1.89 D171E 3.61 N227K 1.50 K308Q 3.73 S56T 1.18 D171K 3.26 Y244M 1.47 K308S 3.21 N59D 1.78 D171Y 2.33 Y244C 1.21 K308Y 1.96 N59C 1.59 D171M 2.01 N252C 1.15 K308G 1.54 N59E 1.51 D171Q 1.57 Q256E 1.54 K308L 1.50 N59R 1.29 D171L 1.37 Q256D 1.31 K308T 1.37 N59F 1.25 I173C 1.41 Q262H 2.32 K308I 1.35 N59W 1.22 D174W 2.05 M263Q 1.13 K309E 5.27 N59V 1.20 D174H 1.69 D264E 1.35 K309G 4.86 N62C 1.69 D174M 1.33 Y265F 3.45 K309C 4.84 N62D 1.42 D174N 1.28 N267T 2.40 K309L 4.16 T63C 1.38 F175Y 1.39 N267S 2.07 K309D 3.99 N68Q 1.11 Q178K 1.91 Y270F 1.22 K309Q 3.97 S72D 2.10 I179T 4.04 H272M 3.49 K309N 3.71 S72E 1.69 S181F 3.68 H272P 2.28 K309T 3.60 I74L 1.11 S181Q 3.41 H272I 1.87 K309A 3.50 S77A 1.91 S181G 2.75 H272V 1.57 K309S 3.48 I82V 1.83 S181N 2.32 N273W 1.13 K309M 2.25 L90F 3.44 S181C 1.68 K274H 2.27 K309H 2.23 E99Q 1.76 S181K 1.59 F276A 4.23 K312E 3.79 L100S 2.05 E185A 4.31 F276N 3.10 K312A 3.11 V106Y 1.53 E185R 1.50 F276K 2.24 K312Q 2.65 T114S 3.12 E185M 1.44 F276C 2.03 K312S 2.39 T114C 1.31 E185V 1.28 I278V 1.59 K312W 2.05 Y123W 1.92 S186K 2.88 S279N 2.85 K312L 1.33 Y124I 2.48 S186M 2.10 S279D 1.21 K312N 1.22 Y124M 2.44 S186R 1.89 S279G 1.17 E314L 1.23 Y124H 2.14 S186H 1.84 D280E 3.19 E314V 1.19 Y124R 2.09 K187G 1.21 D280C 1.17 E314I 1.14 Y124V 1.98 Y188P 2.71 Y281P 4.70 L315I 2.42 Y124Q 1.97 E189V 1.12 Y282N 1.65 R319A 1.21 Y124T 1.38 S199C 1.42 H283I 3.92 Y321F 1.58 N323R 1.20
(1045) Table 1 shows that all the dispersin variants have improved RAR i.e. RAR above 1, and thus are more stable under the tested conditions compared to the dispersin without the above substitutions e.g. compared to the dispersin shown in SEQ ID NO 1.
Example 2b Testing the Variants for Stability
(1046) Growth and Expression
(1047) The constructed variants and the backbone used for the variants were grown in standard 96-well microtiter plates (200 ul broth/well). The used broth for growth and expression was Ca118-2 (Ostergaard P R, Wilting R, Lassen S F. 2010. Identification and characterization of a bacterial glutamic peptidase. BMC Biochem. 11:47) supplemented with 6 ug/ml chloramphenicol. The microtiter plates were grown for 3 days at 30° C. with shaking at 225 rpm. After growth, the plates were centrifuged, and the supernatants were stressed and assayed for stability. All steps regarding growth, stress and assaying are done in 96- or 384-well format microtiter plates.
(1048) Measurement of Stability in the Presence of 80% (v/v) Model A+/−1.0% (v/v) Protease
(1049) The supernatants were diluted 5-fold in concentrated Model A detergent containing 1.25% (v/v) protease (SEQ ID NO 6). After mixing, the samples were split in two parts. One part was incubated for 60 minutes at room temperature (21° C., unstressed sample) whereas the second part was incubated in a PCR machine for 60 minutes at 56° C. (stressed sample). After incubation, the samples were diluted 8-fold in dilution buffer (100 mM Tris-HCl, 0.01% (v/v) TritonX-100, pH 8.0) before the activity of the unstressed and stressed samples was determined by transferring 5 ul sample to a 384-well microtiter plate containing 35 ul assay solution (45 mM citrate buffer pH 5.0 supplemented with 1.0 mg/ml p-nitrophenyl-N-acetyl-β-D-glucosaminide (Sigma Aldrich). After incubation at room temperature for 12 hours, the reactions were stopped by adding 40 μl stop solution (0.4 M Na.sub.2CO.sub.3) and the absorbances at 405 nm (A405) read. For each sample the residual activity (RA) was calculated as: RA=A405(stressed sample)/A405(unstressed sample). All A405 measurements were corrected for the signal of a blank sample (no dispersin present) before calculating the RA. The RA was used to calculate the half-life of the variants and the backbone: Half-life (minutes)=60 (minutes)×Ln(0.5)/Ln(RA) of each variant and the backbone. For each variant the half-life improvement factor (HIF) are calculated as: HIF=Half-life(variant)/Half-life(backbone). Variants with improved stability will have a HIF >1 as the HIF of the backbone in this setup will by definition be 1.0.
(1050) TABLE-US-00003 TABLE 2 Variant HIF Variant HIF Variant HIF Variant HIF D2L 1.49 V106M 1.16 G164D 1.39 D230T 1.20 D2A 1.23 V106D 1.15 G164H 1.26 N233E 1.56 D2W 1.21 V106K 1.15 G164S 1.25 N233D 1.54 D2V 1.20 V106A 1.14 G164E 1.24 N233H 1.28 D2R 1.11 V106Q 1.13 G164V 1.11 N233Q 1.25 D2N 1.11 K107M 1.32 V167Q 2.66 N233R 1.18 Q3L 3.59 K107W 1.30 V167A 1.38 N233W 1.11 Q3V 2.75 K107L 1.23 V167W 1.36 W234R 1.36 Q3Y 2.32 K107C 1.21 V167P 1.32 G235A 1.43 Q3M 2.13 K107V 1.19 V167D 1.26 G235S 1.42 Q3I 1.64 K107A 1.13 V167L 1.22 G235V 1.40 Q3P 1.61 K107T 1.12 V167R 1.20 G235L 1.38 H15Y 2.20 N110R 1.29 V167E 1.18 G235M 1.33 H15F 1.27 N110V 1.12 L170N 1.56 G235E 1.25 T17V 1.55 N110M 1.11 L170R 1.55 G235F 1.24 T17R 1.31 D111V 1.57 L170D 1.43 G235P 1.14 T17M 1.30 D111A 1.29 L170E 1.37 G235I 1.12 T17F 1.30 D111R 1.21 L170K 1.26 G235W 3.10 T17E 1.28 D111N 1.19 L170F 1.26 S237W 2.68 T17C 1.20 D111W 1.17 L170M 1.23 S237Y 1.66 T17W 3.50 D111M 1.12 L170A 1.23 S237G 1.38 E19P 1.25 D111Q 1.11 L170W 1.20 S237M 1.37 E19N 1.21 D111E 1.11 L170Y 1.17 S237N 1.33 E19K 1.10 V113T 1.13 L17V 1.15 S237C 1.25 E19D 1.10 Y116R 1.18 L170Q 1.15 Y244E 1.59 K22A 1.31 Y116D 1.14 L170P 1.12 L249Q 2.38 K22V 1.22 Y116N 1.14 D171L 1.35 L249R 1.65 S23V 1.53 S117N 1.27 D171V 1.31 L249W 1.60 S23R 1.40 S117H 1.21 D171A 1.18 L249H 1.41 S23T 1.34 S117D 1.18 D171W 1.13 L249K 1.14 S23A 1.22 S117P 1.14 D171R 1.11 L249Y 1.11 D26M 1.13 S117* 1.12 D171C 1.10 S251A 1.41 Y30* 1.60 E118G 1.32 D174V 1.43 S251L 1.30 Y30R 1.43 E118* 1.18 N177M 1.12 S251R 1.22 Y30V 1.39 E118A 1.17 Q178R 1.26 S251W 1.14 Y30D 1.31 E118L 1.14 Q178* 1.23 S251N 1.12 Y30A 1.24 E118D 1.12 Q178W 1.23 G253D 1.48 Y30T 1.20 E119W 1.71 Q178K 1.16 G253W 1.16 Y30N 1.16 E119G 1.22 Q178A 1.15 F254I 2.61 Y30L 1.15 T120W 1.76 S181W 1.24 F254L 2.27 Y30M 1.14 T120M 1.55 S181C 1.23 F254M 1.60 G32R 1.70 T120V 1.24 S181V 1.21 F254Y 1.52 G32M 1.32 T120L 1.16 S181D 1.18 Q256R 1.15 G32L 1.23 T120I 1.14 S181T 1.15 N260Q 2.39 N34D 1.45 D122* 1.26 S181P 1.12 N260L 1.80 N43* 1.24 D122R 1.22 G235N 1.32 N260C 1.68 N43L 1.20 H168N 1.22 G235H 1.31 N260R 1.60 N43H 1.13 D122H 1.13 E185W 1.40 N260M 1.55 E44* 1.24 Y124L 1.33 S186R 1.81 N260K 1.51 N45D 1.19 Y124N 1.21 S186D 1.29 N260A 1.47 N45L 1.19 Y124W 1.19 S186E 1.23 N260V 1.41 N45V 1.10 Y124C 1.18 S186N 1.22 N260* 1.37 Y52M 1.13 Y124R 1.16 S186Q 1.18 N260I 1.26 Y52* 1.12 D125H 1.42 S186L 1.15 N260T 1.23 G54N 1.17 N126V 1.11 S186K 1.13 N260E 1.23 G54L 1.13 R127L 1.26 S186W 1.12 N260W 1.18 G54M 1.12 R127W 1.25 S186V 1.12 N260Y 1.14 S56W 1.11 R127Q 1.22 S186I 1.10 E261A 1.34 S57W 1.21 R127M 1.20 K187R 1.31 E261D 1.21 E58N 1.30 R127D 1.15 K187W 1.25 E261R 1.15 N59R 1.43 R127H 1.15 K187V 1.24 E261W 1.12 N59A 1.43 R127K 1.14 K187C 1.22 E261* 1.11 N59C 1.39 V128L 1.22 K187D 1.20 Q262F 2.05 N59M 1.38 V128D 1.15 K187S 1.14 Q262W 1.28 N59D 1.30 V128A 1.14 K187G 1.12 Q262Y 1.26 N59W 1.14 V128C 1.14 E189L 1.23 Q262* 1.22 N59F 1.11 V128T 1.12 E189W 1.13 M263L 1.12 T60V 1.19 D131V 1.15 S199Y 1.27 M263K 1.11 N62D 1.38 Q135M 1.37 S199M 1.19 D264* 1.27 N62W 1.17 Q135* 1.29 S199C 1.16 D264C 1.17 N62Q 1.14 Q135Y 1.16 S199L 1.14 D264N 1.16 N62H 1.10 Q135A 1.14 E200W 1.53 W268E 1.79 T63V 1.34 Q135D 1.13 E200D 1.20 W268R 1.26 T63L 1.32 Q135E 1.13 E200K 1.18 W268C 1.26 T63N 1.31 Q135K 1.11 E200R 1.13 W268M 1.10 T63R 1.23 D138M 1.30 E200M 1.12 A271G 1.27 T63D 1.13 D138V 1.28 E200F 1.11 A271D 1.16 K67L 1.17 D138R 1.27 E200N 1.10 H272M 3.24 K67A 1.15 D138L 1.27 E200L 1.10 H272V 1.85 N68L 1.14 D138W 1.25 A203V 1.37 H272D 1.84 L71R 1.25 D138S 1.14 A203R 1.33 K274R 1.24 L71H 1.22 E139W 1.18 A203G 1.31 K274A 1.14 L71N 1.20 D142W 1.48 A203W 1.26 I278V 2.28 L71W 1.20 D142R 1.24 A203P 1.25 I278Q 1.41 L71V 1.11 Y145V 1.51 A203C 1.21 I278A 1.40 S72T 1.39 Y145N 1.34 A203L 1.20 I278N 1.37 S72G 1.34 Y145* 1.22 A203S 1.17 I278K 1.17 S72C 1.30 Y145H 1.14 A203M 1.17 S279D 3.41 S72M 1.30 Y145L 1.11 A203Q 1.14 S279C 1.52 S72F 1.24 P147L 1.36 A203T 1.13 S279N 1.51 S72N 1.24 P147D 1.27 A203D 1.12 S279G 1.46 S72Y 1.23 P147A 1.26 N204V 1.22 S279E 1.19 S72* 1.23 P147V 1.25 N204W 1.19 Y281P 4.46 S72R 1.17 P147G 1.21 N204M 1.15 Y281H 2.40 S72E 1.16 P147R 1.17 D207N 1.72 Y281A 1.45 S72I 1.14 P147S 1.17 D207V 1.54 Y281K 1.34 D79V 1.11 P147M 1.15 D207R 1.33 Y281N 1.28 K80N 1.36 P147T 1.14 D207Q 1.27 Y281C 1.16 K80R 1.31 P147C 1.14 D207E 1.15 Y281* 1.13 K80W 1.23 P147F 1.11 D207W 1.14 Y282E 1.10 K80E 1.23 K148L 1.20 D207K 1.13 A284V 1.95 K80* 1.21 K148D 1.19 D207A 1.12 A284N 1.29 K80L 1.14 K148A 1.18 S208A 1.59 A284P 1.16 K80V 1.11 K148V 1.16 S208V 1.35 A284T 1.12 K80Q 1.10 F149N 1.28 S208C 1.26 T287N 1.15 K80H 1.10 F149M 1.25 S208W 1.23 S288D 1.56 D81G 1.44 F149L 1.18 S208D 1.20 S288N 1.49 D81A 1.36 E150N 1.48 S208T 1.18 K291L 1.13 D81L 1.33 E150R 1.35 S208Q 1.13 K291R 1.12 D81W 1.29 E150D 1.31 S208G 1.12 K291V 1.11 D81T 1.27 E150K 1.27 S208L 1.12 E300D 1.45 D81V 1.26 E150M 1.21 Q215L 1.24 E300A 1.17 D81R 1.19 E150H 1.20 Q215R 1.17 H301N 1.95 D81S 1.19 E150W 1.18 Q215M 1.16 H301C 1.66 D81N 1.14 E150V 1.18 Q215* 1.11 H301R 1.46 E99R 1.12 E150Y 1.17 S217V 1.84 T303A 1.71 K103R 1.45 E150L 1.15 T218Q 1.83 T303C 1.29 K103V 1.35 E150A 1.12 T218R 1.27 T303K 1.24 K103A 1.20 G151A 1.45 T218A 1.25 T303G 1.24 K104N 1.29 G151P 1.45 T218V 1.20 T303Q 1.22 K104W 1.22 G151W 1.27 T218L 1.10 T303W 1.21 D105N 1.11 G151D 1.25 S221N 1.17 T303R 1.18 V106L 1.36 G151L 1.22 G222D 1.16 K312M 1.26 V106Y 1.28 G151S 1.21 E224A 1.44 K312W 1.10 V106H 1.26 G151N 1.16 E224P 1.28 L315V 1.85 V106E 1.25 G151C 1.15 D230N 1.71 L315I 1.36 V106W 1.24 K152D 1.15 D230R 1.53 V106N 1.23 K152L 1.12 D230* 1.42 V106R 1.20 K152R 1.10 D230W 1.28
(1051) Table 2 shows that all the dispersin variants have improved HIF i.e. HIF above 1, and thus are more stable under the tested conditions compared to the dispersin without the above alterations e.g. compared to the dispersin shown in SEQ ID NO 1.
Example 3 Generation of SSLs and Screening Resulting Libraries as Pools
(1052) Construction of SSL
(1053) A site-saturation library was constructed for each amino acid position in the enzyme and the expected diversity was confirmed by sequencing of the pools of transformants. The pools of grown transformants were stored at −80° C. until use.
(1054) Growth and Expression
(1055) Cultures inoculated from the pools of transformants were grown in deep well plates with 600 μl Cal-18 (Ostergaard P R, Wilting R, Lassen S F. 2010. Identification and characterization of a bacterial glutamic peptidase. BMC Biochem. 11:47) supplemented with 6 μg/ml chloramphenicol at 37° C. for one day with shaking at 700 rpm. The wild type was also inoculated as reference in four wells on each microtiterplate. After growth, the plates were centrifuged to pellet the cells and the variants present in the supernatants were screened for improved stability.
(1056) Testing Dispersin Variants for Stability
(1057) Each supernatant sample was diluted 20-fold in 50 mM Tris-HCl, pH 8.0. The sample was split in two: 5 μl is transferred to a 384-well MTP (unstressed reference) and 10 μl is transferred to 384-well PCR plate. The PCR plate was stressed in a PCR machine at 47° C. for 30 min, while the unstressed reference plate was kept at room temperature (21° C.). After the incubation, 5 μl of the stressed sample was transferred to the 384-well MTP before assaying the activity in all samples by usage of p-nitrophenyl-N-acetyl-β-D-glucosaminide (pNP-NAG).
(1058) Dispersin Activity Assay
(1059) For the dispersin activity measurement, the prepared 384-well microtiter plate containing 5 μl samples was added 35 μl dispersin assay solution (45 mM citrate buffer pH 5 added 0.5 mg/ml p-nitrophenyl-N-acetyl-β-D-glucosaminide). The 384-well plate was then incubated at room temperature for 3 hours. After incubation, 40 μl stop solution (0.4 M Na.sub.2CO.sub.3) was added to the sample and absorbance at 405 nm was measured. The obtained activity values were corrected for the background by subtraction of the absorbance measurement obtained for a reference without dispersin. The residual activity (RA) for each pool of dispersin variants and the reference dispersin was calculated as the ratio of background corrected activity between a stressed and an unstressed sample. These values were subsequently used to calculate the residual activity ratio (RAR.sub.pool=RA.sub.pool/RA.sub.reference), which was used to prioritize of libraries in terms of likelihood of containing stabilized variant(s).
(1060) SSL Hit Selection
(1061) The result obtained for the pool will be the average of all variants contained in the pool and the likelihood of finding improved candidates therefore increase significantly the higher the value of the ratio (RAR.sub.pool). SSLs with highest ratio and above that determined for the reference (i.e. RAR.sub.pool>1) are deemed to be most interesting. In this particular example, six SSL pools with RAR.sub.pool>2 were selected to screen for improved properties amongst isolated clones represented therein.
(1062) Plating, Regrow, Rescreen and Sequence
(1063) The selected variant pools (hits) were plated on LB agar supplemented with 6 μg/ml chloramphenicol and grown for 37° C. for one day. After growth, colonies were picked to individual wells of 96-well deep well plates containing 600 μl Call 8-2 (Ostergaard P R, Wilting R, Lassen S F. 2010. Identification and characterization of a bacterial glutamic peptidase. BMC Biochem. 11:47) broth supplemented with 6 μg/ml chloramphenicol. Dispersin (SEQ ID NO 1), was also inoculated as reference in four wells on each microtiterplate. The microtiter plates were grown for one day at 37° C. with shaking at 700 rpm. After growth, the supernatants were screened for residual activity after stressing them for 30 minutes at 47° C. in 50 mM Tris-HCl, pH 8.0. The following criteria was used for qualification of the collected data. The background corrected activity obtained for the unstressed reference should be at least 0.25. The criteria for selection of hits was based upon the RA obtained for WT, which was determined to 0.1. A sample was considered a hit if its RA.sub.variant/RA.sub.reference equal to RAR>1.
(1064) TABLE-US-00004 TABLE 3a Mutations of position RA.sub.pool: 30 min. 47° C. in SEQ ID NO 1 50 mM Tris-HCl. pH 8.0 RAR.sub.pool SEQ ID NO 1 0.12 1.00 2 0.13 1.11 3 0.24 1.98 20 0.21 1.74 23 0.13 1.09 24 0.16 1.37 29 0.15 1.26 30 0.15 1.25 31 0.22 1.82 33 0.25 2.11 36 0.15 1.26 42 0.19 1.58 43 0.20 1.64 45 0.22 1.82 59 0.27 2.27 72 0.12 1.03 73 0.17 1.41 114 0.12 0.98 124 0.31 2.55 125 0.13 1.09 134 0.14 1.13 135 0.13 1.04 138 0.21 1.77 171 0.15 1.27 174 0.17 1.39 181 0.26 2.18 185 0.16 1.31 186 0.18 1.49 199 0.14 1.17 207 0.20 1.66 215 0.15 1.26 216 0.17 1.39 225 0.23 1.89 227 0.25 2.10 230 0.18 1.52 243 0.15 1.22 244 0.22 1.80 249 0.16 1.30 252 0.15 1.21 253 0.21 1.79 254 0.12 0.98 256 0.12 0.97 261 0.12 1.03 262 0.12 0.96 265 0.16 1.36 272 0.22 1.85 276 0.31 2.56 294 0.12 0.99 303 0.12 1.01 308 0.37 3.05 309 0.55 4.59 312 0.40 3.36 322 0.15 1.25
(1065) Table 3a shows the subset of SSL libraries having improved RAR.sub.pool i.e. RAR.sub.pool above 1, and thus contain a mixture of substitutions giving more stability on average under the tested conditions compared to the dispersin without the substitutions e.g. compared to the dispersin shown in SEQ ID NO 1
(1066) The experiment above was repeated by screening pools made using a stabilized enzyme variant (SEQ ID NO 17) as template during SSL construction but the residual activity ratio (RAR) was measured in model detergent A, with the presence of a protease (SEQ ID NO:16) The results are shown in table 3a* below.
(1067) TABLE-US-00005 TABLE 3a* Mutations of position RAR.sub.pool: 60 min. 64° C. 10 g/L model SEQ ID NO 17 A detergent added 1 v/v % protease SEQ ID NO 17 1.00 1 1.54 3 8.53 6 1.33 57 1.21 73 1.76 75 1.30 90 1.86 94 2.14 116 1.40 141 1.11 142 1.10 144 1.07 147 1.84 149 1.34 169 1.35 170 1.14 193 6.30 205 1.94 217 1.71 225 5.32 232 6.95 233 1.17 241 1.96 243 1.73 253 1.39 254 3.15 261 1.29 267 2.61 272 33.88 276 1.53 277 6.31 278 1.17 279 1.98 281 4.19 284 1.34 289 3.89 296 1.30 297 1.67 299 1.60 301 1.09
(1068) TABLE-US-00006 TABLE 3b Mutations compared to RA: 30 min, 47° C., 50 mM SEQ ID NO 1 Tris-HCl, pH 8.0 RAR SEQ ID NO 1 0.13 1.00 Y124A 0.22 1.70 Y124C 0.63 4.82 Y124F 0.44 3.38 Y124K 0.41 3.13 Y124L 0.44 3.39 Y124M 0.66 5.06 Y124R 0.66 5.03 Y124S 0.16 1.25 Y124V 0.59 4.52 Y124W 0.37 2.82 N227A 0.80 6.09 N227K 0.92 7.04 N227Q 0.93 7.11 N227R 1.11 8.49 N227S 0.79 6.02 N227T 1.23 9.43 F276A 0.90 6.86 F276C 0.93 7.14 F276G 0.24 1.85 F276K 0.44 3.35 F276L 0.17 1.31 F276M 0.39 2.97 F276P 0.38 2.94 F276S 0.35 2.67 F276V 0.23 1.74 F276W 0.38 2.88
(1069) Table 3b shows dispersin variants having improved RAR i.e. RAR above 1, and thus are more stable under the tested conditions compared to the dispersin without the above substitutions e.g. compared to the dispersin shown in SEQ ID NO 1
(1070) The experiment above was repeated by screening selected positions from SSLs made using a stabilized enzyme variant (SEQ ID NO 17) as template but the residual activity ratio (RAR) was measured in model detergent A, with the presence of a protease (SEQ ID NO:16). The results are shown in table 3b* below.
(1071) TABLE-US-00007 Mutations in RAR: 60 min. 63° C. 10 g/L model A SEQ ID NO 17 detergent added 1 v/v % protease SEQ ID NO 17 1 Q3F 9.72 Q3I 8.24 Q3L 8.80 Q3T 8.03 Q3V 5.89 S225G 6.79 E232D 9.03 E232V 9.90 H272M 16.45 H272P 11.91 H272V 12.48 H272W 9.03 S279G 3.60 Y281K 7.64 Y281P 11.25 Y281R 5.80
(1072) TABLE-US-00008 TABLE 3c Mutations compared to RA: 30 min, 47° C., 50 mM SEQ ID NO 1 Tris-HCl, pH 8.0 RAR SEQ ID NO 1 0.17 1.00 K308A 0.38 2.23 K308D 0.54 3.19 K308E 0.62 3.64 K308G 0.26 1.53 K308S 0.34 2.02 K308T 0.28 1.63 K308V 0.17 1.02 K308Y 0.17 1.01 K309A 0.35 2.07 K309C 0.53 3.10 K309E 0.56 3.30 K309G 0.49 2.88 K309H 0.23 1.38 K309I 0.18 1.07 K309L 0.19 1.11 K309N 0.35 2.08 K309Q 0.47 2.78 K309S 0.35 2.06 K309T 0.34 1.98 K312A 0.43 2.52 K312E 0.66 3.93 K312L 0.27 1.59 K312N 0.31 1.85 K312Q 0.48 2.85 K312S 0.45 2.65
(1073) Table 3c shows dispersin variants having improved RAR i.e. RAR above 1, and thus are more stable under the tested conditions compared to the dispersin without the above substitutions e.g. compared to the dispersin shown in SEQ ID NO 1
Example 4 Combination Variants with 1-3 Mutations
(1074) Growth and Expression
(1075) The constructed variants and the backbone used for the variants were grown in standard 96-well microtiter plates 200 ul Ca118-2 broth (Ostergaard P R, Wilting R, Lassen S F. 2010. Identification and characterization of a bacterial glutamic peptidase. BMC Biochem. 11:47) supplemented with 6 ug/ml chloramphenicol broth/well. The microtiter plates were grown for 3 days at 30° C. with shaking at 225 rpm. After growth, the plates were centrifuged and the supernatants were stressed and assayed for stability. All steps regarding growth, stress and assaying are done in 96- or 384-well format microtiter plates.
(1076) Measuring Stability at Elevated Temperature
(1077) Each supernatant sample was diluted 10-fold in stress buffer (100 mM Tris-HCl, 0.01% (v/v) TritonX-100, pH 8.0. After mixing, the samples were split in four and two samples incubated at room temperature (21° C., unstressed samples) and the other two samples (stressed samples) incubated in a PCR machine for 120 minutes at 41° C. and 46° C., respectively. After incubation, the activity of the un-stressed and stressed samples was determined by transferring 5 ul of each sample to a 384-well microtiter plate containing 35 ul assay solution (45 mM citrate buffer pH 5.0 supplemented with 1.0 mg/ml p-nitrophenyl-N-acetyl-β-D-glucosaminide (Sigma Aldrich). After incubation at room temperature for 3 hours, the reactions were stopped by adding 40 μl stop solution (0.4 M Na.sub.2CO.sub.3) and the absorbances at 405 nm (A405 nm) read. For each sample the residual activity (RA) was calculated as: RA=A405(stressed sample)/A405(unstressed sample). All A405 nm measurements were corrected for the signal of a blank sample (no dispersin present) before calculating the RA. A variant is improved in stability if RA(variant)>RA(backbone).
(1078) TABLE-US-00009 TABLE 4 RA (residual activity) Number of 41° C. 46° C. substitutions Mutations 120 min 120 min 0 SEQ ID NO 1 0.07 0.01 1 F276A 0.90 0.17 1 K308E 0.92 0.10 1 K309E 0.94 1 K312E 0.91 0.13 1 N227T 0.78 0.05 1 N252P 0.45 0.02 1 N267T 0.56 0.01 1 Q215K 0.63 0.03 1 S163P 0.48 0.02 2 F276A + K308E 0.98 0.98 2 F276A + K309E 1.00 1.00 2 K308E + K309E 0.97 0.97 2 K308E + K312E 0.99 0.88 2 K309E + K312E 1.00 0.93 2 N227T + F276A 1.00 0.98 2 N227T + K308E 0.97 0.94 2 N227T + K312E 0.99 0.94 2 N227T + N252P 1.00 0.15 2 N227T + N267T 0.94 0.40 2 N252P + F276A 0.98 0.97 2 N252P + K308E 1.00 0.26 2 N252P + K309E 0.99 0.81 2 N252P + N267T 0.75 0.02 2 N267T + F276A 0.97 0.63 2 N267T + K308E 1.00 0.32 2 N267T + K309E 0.98 0.79 2 N267T + K312E 0.97 0.59 2 Q215K + F276A 1.00 0.79 2 Q215K + K308E 0.94 0.62 2 Q215K + K309E 0.98 0.75 2 Q215K + K312E 0.97 0.71 2 Q215K + N227T 0.98 0.60 2 Q215K + N252P 0.83 0.03 2 Q215K + N267T 0.96 0.05 2 S163P + F276A 0.97 0.79 2 S163P + K308E 0.96 0.75 2 S163P + K309E 0.96 0.83 2 S163P + K312E 0.97 0.88 2 S163P + N252P 0.96 0.05 2 S163P + N267T 0.64 0.03 2 S163P + Q215K 0.91 0.14 3 F276A + K308E + K309E 0.97 0.96 3 F276A + K308E + K312E 0.99 1.00 3 F276A + K309E + K312E 1.00 1.00 3 K308E + K309E + K312E 1.00 0.99 3 N227T + F276A + K309E 0.99 0.97 3 N227T + F276A + K312E 0.99 0.98 3 N227T + K308E + K309E 0.99 1.00 3 N227T + K308E + K312E 1.00 1.00 3 N227T + K309E + K312E 1.00 0.99 3 N227T + N252P + K308E 1.00 1.00 3 N227T + N252P + K309E 0.99 0.99 3 N227T + N252P + K312E 1.00 1.00 3 N227T + N252P + N267T 1.00 1.00 3 N227T + N267T + F276A 1.00 0.99 3 N227T + N267T + K308E 0.99 0.97 3 N227T + N267T + K309E 0.97 0.96 3 N227T + N267T + K312E 1.00 0.89 3 N252P + F276A + K308E 0.96 0.97 3 N252P + F276A + K309E 1.00 0.99 3 N252P + F276A + K312E 0.98 0.97 3 N252P + K308E + K309E 0.99 0.93 3 N252P + K308E + K312E 0.97 0.95 3 N252P + N267T + F276A 1.00 0.97 3 N252P + N267T + K308E 1.00 1.00 3 N252P + N267T + K309E 1.00 1.00 3 N252P + N267T + K312E 0.99 0.98 3 N267T + F276A + K308E 1.00 1.00 3 N267T + F276A + K309E 0.99 1.00 3 N267T + F276A + K312E 0.98 0.97 3 N267T + K308E + K309E 1.00 1.00 3 N267T + K308E + K312E 0.97 0.98 3 N267T + K309E + K312E 1.00 0.98 3 Q215K + F276A + K308E 0.98 0.92 3 Q215K + F276A + K312E 1.00 1.00 3 Q215K + K308E + K312E 0.99 0.94 3 Q215K + K309E + K312E 0.98 0.98 3 Q215K + N227T + F276A 1.00 1.00 3 Q215K + N227T + K308E 0.99 0.99 3 Q215K + N227T + K309E 0.97 0.97 3 Q215K + N227T + K312E 0.96 0.96 3 Q215K + N227T + N252P 0.96 0.78 3 Q215K + N227T + N267T 0.96 0.86 3 Q215K + N252P + F276A 1.00 0.96 3 Q215K + N252P + K309E 0.98 0.93 3 Q215K + N252P + K312E 0.99 0.95 3 Q215K + N252P + N267T 0.97 0.23 3 Q215K + N267T + F276A 0.99 0.97 3 Q215K + N267T + K312E 1.00 0.96 3 S163P + F276A + K308E 1.00 1.00 3 S163P + F276A + K312E 0.98 0.96 3 S163P + K308E + K309E 0.98 0.97 3 S163P + K308E + K312E 1.00 1.00 3 S163P + K309E + K312E 0.99 1.00 3 S163P + N227T + F276A 0.96 0.96 3 S163P + N227T + K309E 1.00 1.00 3 S163P + N227T + N252P 1.00 0.98 3 S163P + N227T + N267T 0.97 0.86 3 S163P + N252P + F276A 1.00 0.99 3 S163P + N252P + K308E 0.97 0.91 3 S163P + N252P + K309E 0.99 0.98 3 S163P + N252P + K312E 0.98 0.96 3 S163P + N252P + N267T 0.97 0.57 3 S163P + N267T + K308E 0.91 0.90 3 S163P + N267T + K309E 0.99 0.99 3 S163P + N267T + K312E 0.98 0.96 3 S163P + Q215K + F276A 0.99 0.95 3 S163P + Q215K + K308E 1.00 0.94 3 S163P + Q215K + K309E 0.63 0.95 3 S163P + Q215K + K312E 0.94 0.95 3 S163P + Q215K + N227T 0.95 0.90 3 S163P + Q215K + N252P 0.94 0.38
(1079) Table 4 shows the effect of adding several substitutions compared to SEQ ID NO 1. It is shown that the single stabilizing substitutions can be combined to generate a variant which is more stable or on par to the corresponding variant with one less substitution.
Example 5 Combination Variants with 4-9 Mutations
(1080) Growth and Expression
(1081) The constructed variants and the backbone used for the variants were grown in either 96-deep-Well (600 ul broth/well) or standard 96-well microtiter plates (200 ul broth/well). The used broth for growth and expression was Ca118-2 (Ostergaard P R, Wilting R, Lassen S F. 2010. Identification and characterization of a bacterial glutamic peptidase. BMC Biochem. 11:47) supplemented with 6 ug/ml chloramphenicol. The plates were grown for 1 day at 37° C. with shaking at 700 rpm (96-well deepwell plates) or for 3 days at 30° C. with shaking at 225 rpm (standard 96-well microtiter plates). After growth, the plates were centrifuged and the supernatants were stressed and assayed for stability. All steps regarding growth, stress and assaying are done in 96- or 384-well format microtiter plates.
(1082) Measurement of Stability in the Presence of 0.9 g/L Model A+/−0.9% (v/v) Protease
(1083) Each supernatant sample was diluted 10-fold in stress buffer (100 mM Tris-HCl, 0.01% (v/v) TritonX-100, pH 8.0 supplemented with 1 g/L Model A detergent). In the cases with applied protease stress, the stress buffer was also added 1% (v/v) protease (SEQ ID NO 7). After mixing, the sample was split into an unstressed sample which was incubated at room temperature (21° C.) and a stressed sample which was incubated in a PCR machine for 30 minutes at the selected stress temperatures (50° C., 51° C., 54° C., 57° C. and/or 60° C.). After the incubation, the activity of the un-stressed and stressed sample was determined by transferring 5 ul of each sample to a 384-well microtiter plate containing 35 ul assay solution (45 mM citrate buffer pH 5.0 supplemented with 1.0 mg/ml p-nitrophenyl-N-acetyl-β-D-glucosaminide (Sigma Aldrich). After 3 hour incubation at room temperature, the reactions were stopped by adding 40 μl stop solution (0.4 M Na.sub.2CO.sub.3) and the absorbances at 405 nm (A405) read. For each sample the residual activity (RA) was calculated as: RA=A405(stressed sample)/A405(unstressed sample). All A405 measurements were corrected for the signal of a blank sample (no dispersin present) before calculating the RA. A variant stabilized at the tested stress conditions is defined as a variant having a RA higher than the used backbone. All variants were tested in 30 min in 0.9 g/L Model A detergent.
(1084) TABLE-US-00010 TABLE 5 RA RA (residual (residual activity) Number of activity) 50° C. + 0.9% substitutions Substitutions compared to SEQ ID NO 1 50° C. Protease 0 SEQ ID NO 1 <0.05 <0.05 4 N227T + F276A + K308E + K309E 0.78 0.37 4 N227T + F276A + K308E + K312E 0.87 0.44 4 N227T + F276A + K309E + K312E 0.89 0.42 4 N227T + N267T + F276A + K308E 0.76 0.38 4 N227T + N267T + F276A + K309E 0.86 0.45 4 N227T + N267T + F276A + K312E 0.82 0.45 4 S163P + N227T + F276A + K308E 0.55 0.39 5 N227T + N267T + F276A + K308E + 0.92 0.54 K309E 5 N227T + N267T + F276A + K308E + 0.94 0.51 K312E 5 N227T + N267T + F276A + K309E + 0.92 0.50 K312E 5 S163P + N227T + F276A + K308E + 0.90 0.74 K312E 5 N227T + F276A + K308E + K309E + 0.92 0.53 K312E 6 N227T + N267T + F276A + K308E + 0.94 0.53 K309E + K312E
(1085) TABLE-US-00011 TABLE 6 RA RA (residual (residual activity) activity) Number of 51° C. 0.9% 54° C. 0.9% substitutions Substitutions compared to SEQ ID NO 1 Protease Protease 5 N227T + F276A + K308E + K309E + K312E 0.36 0.24 6 N227T + N267T + F276A + K308E + K309E + K312E 0.35 0.18 6 N227T + N252P + F276A + K308E + K309E + K312E 0.49 0.25 6 Q215K + N227T + F276A + K308E + K309E + K312E 0.50 0.36 6 S163P + N227T + F276A + K308E + K309E + K312E 0.71 0.63 7 N227T + N252P + N267T + F276A + K308E + K309E + K312E 0.41 0.26 7 Q215K + N227T + N252P + F276A + K308E + K309E + K312E 0.45 0.31 7 Q215K + N227T + N267T + F276A + K308E + K309E + K312E 0.51 0.32 7 S163P + N227T + N252P + F276A + K308E + K309E + K312E 0.80 0.74 7 S163P + N227T + N267T + F276A + K308E + K309E + K312E 0.75 0.72 7 S163P + Q215K + N227T + F276A + K308E + K309E + K312E 0.76 0.72 8 Q215K + N227T + N252P + N267T + F276A + K308E + K309E + 0.41 0.31 K312E 8 S163P + N227T + N252P + N267T + F276A + K308E + K309E + 1.02 0.81 K312E 8 S163P + Q215K + N227T + N252P + F276A + K308E + K309E + 0.81 0.77 K312E 8 S163P + Q215K + N227T + N267T + F276A + K308E + K309E + 0.81 0.72 K312E 9 S163P + Q215K + N227T + N252P + N267T + F276A + K308E + 0.81 0.78 K309E + K312E
(1086) TABLE-US-00012 TABLE 7 RA RA (residual (residual activity) activity) Number of 57° C. 0.9% 60° C. 0.9% substitutions Substitutions compared to SEQ ID NO 1 Protease Protease 5 N227T + F276A + K308E + K309E + K312E 0.09 6 N227T + N267T + F276A + K308E + K309E + K312E 0.09 6 N227T + N252P + F276A + K308E + K309E + K312E 0.13 0.11 6 Q215K + N227T + F276A + K308E + K309E + K312E 0.16 0.10 6 S163P + N227T + F276A + K308E + K309E + K312E 0.30 0.11 7 N227T + N252P + N267T + F276A + K308E + K309E + 0.10 0.08 K312E 7 Q215K + N227T + N252P + F276A + K308E + K309E + 0.20 0.17 K312E 7 Q215K + N227T + N267T + F276A + K308E + K309E + 0.14 0.10 K312E 7 S163P + N227T + N252P + F276A + K308E + K309E + 0.54 0.37 K312E 7 S163P + N227T + N267T + F276A + K308E + K309E + 0.44 0.27 K312E 7 S163P + Q215K + N227T + F276A + K308E + K309E + 0.50 0.30 K312E 8 Q215K + N227T + N252P + N267T + F276A + K308E + 0.15 0.11 K309E + K312E 8 S163P + N227T + N252P + N267T + F276A + K308E + 0.60 0.40 K309E + K312E 8 S163P + Q215K + N227T + N252P + F276A + K308E + 0.66 0.46 K309E + K312E 8 S163P + Q215K + N227T + N267T + F276A + K308E + 0.57 0.45 K309E + K312E 9 S163P + Q215K + N227T + N252P + N267T + F276A + 0.58 0.46 K308E + K309E + K312E
(1087) Table 5, 6 and 7 shows the effect of adding several substitutions compared to SEQ ID NO 1. It is shown that the single stabilizing substitutions can be combined to generate a variant which is more or on par in stability as the corresponding variant with one less substitution.
Example 6 Testing for Thermostability
(1088) Growth and Expression
(1089) The variants were grown and expressed as described in the previous examples and purified as described below.
(1090) Purification
(1091) The culture broth was centrifuged (26000×g, 20 min) and the supernatant was carefully decanted from the precipitate. The supernatant was filtered through a Nalgene 0.2 μm filtration unit to remove the rest of the Bacillus host cells. The 0.2 μm filtrate was transferred to 20 mM MES/NaOH, pH 6.0 on a G25 sephadex column (from GE Healthcare). The G25 transferred solution was applied to a SOURCE Q column (from GE Healthcare) equilibrated in 20 mM MES/NaOH, pH 6.0. After washing the column extensively with the equilibration buffer, the protease was eluted with a linear NaCl gradient (0.fwdarw.1.0M NaCl) in the same buffer over five column volumes. Fractions were collected during elution and the collected fractions were analysed by SDS-PAGE. Fractions, where only one band was seen after coomassie staining, were pooled as the purified preparation and was used for further experiments.
(1092) Measurement of Stability
(1093) Nano differential scanning fluorimetry (nDSF) was utilized to determine thermal stability. Dispersin variants for nDSF were diluted to ˜0.2 mg/mL (>10-fold dilution) in 50 mM 2-(N-Morpholin)ethansulfonsyre (MES), 50 mM glycine, 50 mM acetic acid, pH 7 or 50 mM MES, 50 mM glycine, 50 mM acetic acid, pH 7 and x (x=2 or 50) g/L Model A (model detergent A). Sample dilution was made in 96-well microtiter plates and transferred to 384-well microplates. The instruments utilized for nDSF experiments, were either a Prometheus NT.48 or Prometheus NT.Plex with autosampler from Nanotemper. With the Prometheus NT.48, the samples were loaded manually using single capillaries (PR-0002), while samples were loaded in the Prometheus NT.Plex using capillary chips (PR-AC002). The experiments were conducted from 20 to 95° C. with a temperature gradient of 3.3° C./min. The melting temperatures (Tm-values) were obtained from peak values derived from the first-derivative of the signal trace (350/330 nm fluorescence ratio or 330 nm fluorescence) using PR. ThermControl software.
(1094) TABLE-US-00013 TABLE 8 Thermostability for variants Tm-values (° C.) 0 g/L 2 g/L 50 g/L Substitutions compared to SEQ ID NO 1 Model A Model A Model A SEQ ID NO 1 50.53 40.94 F276A 55.22 53.86 52.36 K308E 54.14 K309E 55.48 52.75 46.62 K312E 54.96 52.23 N227T 53.11 41.30 N227T + F276A 58.00 55.99 49.87 N227T + F276A + K308E 63.38 62.25 58.99 N227T + F276A + K309E 64.28 64.05 60.35 N227T + F276A + K312E 63.71 63.78 60.70 N227T + F276A + K308E + K309E 68.45 67.73 65.24 N227T + F276A + K308E + K312E 67.19 66.32 63.88 N227T + F276A + K309E + K312E 67.37 66.9 64.57 N227T + N267T + F276A + K308E 64.71 55.32 63.10 N227T + N267T + F276A + K309E 66.45 66.79 64.72 N227T + N267T + F276A + K312E 65.86 66.59 65.07 N227T + F276A + K308E + K309E + K312E 68.42 67.96 66.06 N227T + N267T + F276A + K308E + K309E 70.36 69.86 68.2 N227T + N267T + F276A + K308E + K312E 69.33 68.89 66.83 S163P + N227T + F276A + K308E + K312E 67.59 66.78 63.81 N227T + N267T + F276A + K308E + K309E + K312E 70.53 70.54 68.88 S163P + Q215K + N227T + N252P + N267T + 76.12 75.87 73.98 F276A S163P + N227T + N252P + F276A + K308E + 72.74 72.69 70.67 K309E + K312E S163P + N227T + N267T + F276A + K308E + 71.27 71.04 68.87 K309E + K312E S163P + Q215K + N227T + F276A + K308E + 71.42 68.59 K309E + K312E S163P + Q215K + N227T + N252P + N267T + 70.63 69.74 66.42 F276A + K308E S163P + Q215K + N227T + N252P + N267T + 73.46 73.41 69.96 F276A + K309E S163P + Q215K + N227T + N252P + N267T + 72.49 71.59 69.04 F276A + K312E Q215K + N227T + N252P + N267T + F276A + 75.79 75.53 74.29 K308E + K309E + K312E S163P + N227T + N252P + N267T + F276A + 74.87 74.82 73.40 K308E + K309E + K312E S163P + Q215K + N227T + N252P + F276A + 75.10 75.02 73.01 K308E + K309E + K312E S163P + Q215K + N227T + N252P + N267T + 76.61 75.93 73.57 F276A + K308E + K309E S163P + Q215K + N227T + N252P + N267T + 75.74 75.5 73.56 F276A + K309E + K312E S163P + Q215K + N227T + N252P + N267T + 72.05 71.6 68.52 K308E + K309E + K312E S163P + Q215K + N227T + N267T + F276A + 73.07 72.92 70.98 K308E + K309E + K312E S163P + Q215K + N252P + N267T + F276A + 72.68 72.66 70.85 K308E + K309E + K312E S163P + Q215K + N227T + N252P + N267T + 76.77 76.72 75.07 F276A + K308E + K309E + K312E
(1095) TABLE-US-00014 TABLE 8a Tm-values (° C.) 0 g/L 2 g/L 50 g/L Substitutions compared to SEQ ID NO 1 Model A Model A Model A SEQ ID NO 1 50.53 40.94 N.D. S186R + N227T + E232D + K308E 59.60 57.39 50.18 S163P + Q215K + N227T + N252P + N267T + F276A + K308Q + K309E + K312E 65.25 65.08 59.92 S163P + Q215K + N227T + N252P + N267T + F276A + K308E + K309E + K312Q 76.80 76.42 74.66 Q3F + A49W + N59E + S163P + S186R + N227T + E232D + N252P + F276A + 77.70 77.49 76.00 S279G + K308E + K309E + K312E A49W + N59E + S163P + S186R + S225G + N227T + E232D + N252P + F276A + 77.84 77.93 76.16 S279G + K308E + K309E + K312E Q3F + T17W + A49W + N59E + S163P + S186R + S225G + N227T + G235W + 78.25 77.67 74.95 N252P + H272P + Y281P + K308Q + K309E + K312Q A49W + N59E + S163P + S186R + N227T + E232D + N252P + H272V + F276A + 78.39 78.31 76.49 S279G + K308E + K309E + K312E Q3F + A49W + N59E + S163P + S186R + S225G + N227T + N252P + F276A + 78.65 78.33 76.84 S279G + K308E + K309E + K312E A49W + N59E + S163P + S186R + N227T + E232D + N252P + F276A + S279G + 78.68 78.13 76.54 Y281P + K308E + K309E + K312E A49W + N59E + S163P + S186R + S225G + N227T + N252P + F276A + S279G + 79.16 79.12 77.89 Y281P + K308E + K309E + K312E Q3F + A49W + N59E + S163P + S186R + S225G + N227T + 79.71 79.56 78.05 E232D + N252P + F276A + S279G + K308E + K309E + K312E Q3F + S163P + S186R + Q215K + N227T + E232D + N252P + 80.17 79.52 78.43 N267T + F276A + S279G + K308E + K309E + K312E H15Y + A49W + N59E + S163P + S186R + S225G + N227T + E232D + G235W + 80.19 80.20 78.91 N252P + N260Q + H272P + S279D + Y281P + K308Q + K309E + K312Q Q3F + S163P + S186R + Q215K + S225G + N227T + N252P + N267T + F276A + 80.36 79.76 78.52 S279G + K308E + K309E + K312E H15Y + A49W + N59E + S163P + S186R + S225G + N227T + E232D + N252P + 80.42 81.57 78.94 N260Q + H272P + S279D + Y281P + S288P + K308Q + K309E + K312Q A49W + N59E + S163P + S186R + S225G + N227T + E232D + N252P + H272V + 80.76 80.61 79.42 F276A + S279G + K308E + K309E + K312E H15Y + A49W + N59E + S163P + S186R + S225G + N227T + E232D + G235W + 80.94 81.12 79.29 N252P + N260Q + H272V + S279D + Y281P + K308Q + K309E + K312Q S163P + S186R + Q215K + N227T + E232D + N252P + 80.97 80.09 79.23 N267T + F276A + S279G + Y281P + K308E + K309E + K312E Q3F + A49W + N59E + S163P + S186R + N227T + E232D + N252P + F276A + 80.99 80.56 79.67 S279G + Y281P + K308E + K309E + K312E H15Y + A49W + N59E + S163P + S186R + S225G + N227T + E232D + N252P + 81.15 81.32 79.56 N260Q + H272P + S279D + Y281P + K308Q + K309E + K312Q A49W + N59E + S163P + S186R + S225G + N227T + E232D + N252P + F276A + 81.16 81.06 79.84 S279G + Y281P + K308E + K309E + K312E H15Y + A49W + N59E + S163P + S186R + S225G + N227T + E232D + N252P + 81.17 80.92 77.86 N260Q + H272V + Y281P + K308Q + K309E + K312Q Q3F + T17W + A49W + N59E + S163P + S186R + D207N + T218Q + S225G + 81.18 80.71 78.60 N227T + G235W + N252P + H272P + Y281P + K308Q + K309E + K312Q H15Y + T17W + A49W + N59E + S163P + S186R + S225G + N227T + E232D + 81.24 81.19 77.87 G235W + N252P + N260Q + H272V + S279D + Y281P + K308Q + K309E + K312Q Q3F + A49W + N59E + S163P + S186R + S225G + N227T + N252P + F276A + 81.76 81.55 80.60 S279G + Y281P + K308E + K309E + K312E Q3F + A49W + N59E + S163P + S186R + S225G + N227T + E232D + N252P + 81.81 81.59 80.51 H272V + F276A + S279G + K308E + K309E + K312E A49W + N59E + S163P + S186R + N227T + E232D + N252P + 81.87 81.46 80.62 H272V + F276A + S279G + Y281P + K308E + K309E + K312E S163P + S186R + S225G + N227T + E232D + N252P + 81.93 83.27 81.14 N267T + F276A + S279G + Y281P + K308E + K309E + K312E H15Y + T17W + A49W + N59E + S163P + S186R + S225G + N227T + E232D + 81.96 81.81 79.45 N252P + N260Q + H272V + S279D + Y281P + K308Q + K309E + K312Q Q3F + S163P + S186R + Q215K + N227T + E232D + N252P + 82.12 81.36 80.19 N267T + H272V + F276A + S279G + K308E + K309E + K312E Q3I + H15Y + A49W + N59E + S163P + S186R + S225G + 82.13 81.51 79.96 N227T + E232D + G235W + N252P + N260Q + H272V + S279D + Y281P + K308Q + K309E + K312Q S163P + S186R + Q215K + S225G + N227T + E232D + 82.15 81.36 80.60 N252P + N267T + F276A + S279G + Y281P + K308E + K309E + K312E S163P + Q215K + S225G + N227T + E232D + N252P + 82.16 81.33 80.43 N267T + F276A + S279G + Y281P + K308E + K309E + K312E S163P + S186R + Q215K + S225G + N227T + E232D + 82.28 81.08 79.79 N252P + N267T + H272V + F276A + S279G + K308E + K309E + K312E S163P + Q215K + S225G + N227T + E232D + N252P + 82.39 81.76 80.60 N267T + H272V + F276A + S279G + K308E + K309E + K312E A49W + N59E + S163P + S186R + S225G + N227T + N252P + H272V + 82.60 82.50 81.14 F276A + S279G + Y281P + K308E + K309E + K312E Q3F + S163P + S186R + Q215K + N227T + E232D + N252P + N267T + 82.61 83.55 81.68 F276A + S279G + Y281P + K308E + K309E + K312E Q3F + S163P + Q215K + N227T + E232D + N252P + N267T + F276A + 82.69 81.60 80.72 S279G + Y281P + K308E + K309E + K312E Q3F + H15Y + T17W + A49W + N59E + S163P + S186R + 82.78 83.35 80.07 T218Q + S225G + N227T + G235W + S237W + N252P + H272P + Y281P + K308Q + K309E + K312Q S163P + Q215K + N227T + E232D + N252P + N267T + 82.84 84.19 82.34 H272V + F276A + S279G + Y281P + K308E + K309E + K312E Q3F + S163P + Q215K + S225G + N227T + E232D + N252P + 82.91 81.69 80.47 N267T + H272V + F276A + S279G + K308E + K309E + K312E Q3F + A49W + N59E + S163P + S186R + N227T + E232D + 82.94 82.48 81.61 N252P + H272V + F276A + S279G + Y281P + K308E + K309E + K312E Q3F + S163P + S186R + Q215K + S225G + N227T + N252P + N267T + 83.18 82.41 81.62 F276A + S279G + Y281P + K308E + K309E + K312E Q3F + H15Y + T17W + A49W + N59E + S163P + S186R + 83.42 84.07 80.61 D207N + T218Q + S225G + N227T + G235W + S237W + N252P + H272P + Y281P + K308Q + K309E + K312Q S163P + S186R + Q215K + S225G + N227T + N252P + 83.47 84.70 83.22 N267T + H272V + F276A + S279G + Y281P + K308E + K309E + K312E S163P + Q215K + S225G + N227T + N252P + N267T + 83.50 82.53 81.62 H272V + F276A + S279G + Y281P + K308E + K309E + K312E Q3F + S163P + Q215K + N227T + E232D + N252P + N267T + H272V + 83.62 82.52 82.27 F276A + S279G + Y281P + K308E + K309E + K312E Q3F + A49W + N59E + S163P + S186R + S225G + N227T + E232D + 83.65 82.73 81.76 N252P + F276A + S279G + Y281P + K308E + K309E + K312E Q3F + A49W + N59E + S163P + S186R + S225G + N227T + N252P + H272V + 83.85 83.63 82.18 F276A + S279G + Y281P + K308E + K309E + K312E A49W + N59E + S163P + S186R + S225G + N227T + E232D + N252P + H272V + 83.97 83.44 82.73 F276A + S279G + Y281P + K308E + K309E + K312E S163P + Q215K + S225G + N227T + E232D + N252P + 84.12 82.91 82.08 N267T + H272V + F276A + S279G + Y281P + K308E + K309E + K312E Q3F + S163P + S186R + Q215K + S225G + N227T + E232D + N252P + N260Q + 84.21 84.61 82.56 N267T + H272P + S279D + Y281P + K308Q + K309E + K312Q Q3F + S163P + S186R + Q215K + N227T + E232D + N252P + N267T + H272V + 84.33 83.00 82.34 F276A + S279G + Y281P + K308E + K309E + K312E S163P + S186R + Q215K + S225G + N227T + E232D + 84.55 85.90 84.65 N252P + N267T + H272V + F276A + S279G + Y281P + K308E + K309E + K312E Q3F + S163P + S186R + Q215K + S225G + N227T + N252P + N267T + H272V + 84.62 83.59 82.73 F276A + S279G + Y281P + K308E + K309E + K312E Q3F + H15Y + V140I + S163P + S186R + Q215K + S225G + N227T + E232D + 84.79 83.58 82.43 G235W + N252P + N260Q + N267T + H272V + S279D + Y281P + K308Q + K309E + K312Q Q3F + T17W + S163P + S186R + Q215K + S225G + N227T + E232D + G235W + 84.80 84.93 83.17 N252P + N267T + H272V + F276A + S279G + Y281P + K308E + K309E + K312Q Q3F + S163P + S186R + Q215K + S225G + N227T + E232D + N252P + N267T + 85.08 85.62 83.87 H272V + F276A + S279G + Y281P + K308E + K309E + K312Q Q3F + A49W + N59E + S163P + S186R + S225G + N227T + E232D + N252P + 85.15 84.60 83.54 H272V + F276A + S279G + Y281P + K308E + K309E + K312E Q3F + S163P + S186R + Q215K + S225G + N227T + E232D + N252P + N267T + 85.19 84.99 83.88 H272V + F276A + S279G + Y281P + K308E + K309E + K312E Q3F + H15Y + T17W + S163P + S186R + Q215K + S225G + N227T + E232D + 85.20 85.06 83.15 G235W + N252P + N260Q + N267T + H272V + S279D + Y281P + K308Q + K309E + K312Q Q3F + S163P + Q215K + S225G + N227T + E232D + N252P + N267T + H272V + 85.34 86.79 84.79 F276A + S279G + Y281P + K308E + K309E + K312E Q3F + H15Y + S163P + S186R + Q215K + S225G + N227T + E232D + G235W + 85.53 85.89 84.59 N252P + N260Q + N267T + H272P + S279D + Y281P + K308Q + K309E + K312Q Q3F + A49W + N59E + S163P + S186R + S225G + N227T + E232D + G235W + 85.71 87.50 86.35 N252P + N260Q + H272P + F276A + S279D + Y281P + K308E + K309E + K312Q Q3F + H15Y + T17W + S163P + S186R + Q215K + S225G + N227T + E232D + 86.24 86.09 83.78 N252P + N260Q + N267T + H272V + S279D + Y281P + K308Q + K309E + K312Q Q3F + H15Y + S163P + S186R + Q215K + S225G + N227T + E232D + N252P + 86.42 87.01 85.52 N260Q + N267T + H272P + S279D + Y281P + K308Q + K309E + K312Q Q3F + H15Y + S163P + S186R + Q215K + S225G + N227T + E232D + G235W + 86.61 86.96 85.35 N252P + N267T + H272V + F276A + S279G + Y281P + K308Q + K309E + K312Q Q3F + H15Y + T17W + S163P + S186R + Q215K + S225G + N227T + E232D + 86.96 86.83 84.64 N252P + N267T + H272V + F276A + S279G + Y281P + K308Q + K309E + K312Q Q3F + H15Y + A49W + N59E + V140I + S163P + S186R + Q215K + S225G + 87.16 85.92 84.43 N227T + E232D + G235W + N252P + N260Q + N267T + H272V + S279D + Y281P + K308Q + K309E + K312Q
(1096) Table 8+8a show that adding substitutions mentioned to SEQ ID NO: 1 generally increase the Tm (melting temperature) value both in a buffer system and in buffer supplemented with the detergent Model A. A Tm increase is here used as evaluation for increased stability of the variants.
(1097) TABLE-US-00015 TABLE 9 Thermostability for variants comprising di-sulphide bridges Effect of A269C − Y282C nDSF Delta Delta Di- Tm (° C.) Tm (° C.) Tm Tm sulphide 0 g/L 2 g/L 0 g/L 2 g/L Substitution Base Substitution Bridge Model A ModelA Model A Model A A49W + F276A + A49W + F276A + No 58.79 58.14 5.30 5.59 K308E K308E Disulfide Bridge A49W + A269C + A49W + F276A + A269C + 64.09 63.73 F276A + Y282C + K308E Y282C K308E A49W + N227T + A49W + N227T + No 58.97 56.27 5.73 7.01 F276A F276A Disulfide Bridge A49W + N227T + A49W + N227T + A269C + 64.70 63.28 A269C + F276A + F276A Y282C Y282C A49W + N227T + A49W + N227T + No 63.06 63.21 5.66 4.83 F276A + K308E F276A + K308E Disulfide Bridge A49W + N227T + A49W + N227T + A269C + 68.72 68.04 A269C + F276A + F276A + K308E Y282C Y282C + K308E A49W + N227T + A49W + N227T + A269C + 64.5 63.08 ND ND A269C + Y282C + K308E Y282C K308E A49W + S186R + A49W + S186R + No 60.13 56.72 4.32 6.39 N227T + F276A N227T + F276A Disulfide Bridge A49W + S186R + A49W + S186R + A269C + 64.45 63.11 N227T + A269C + N227T + F276A Y282C F276A + Y282C A49W + S186R + A49W + S186R + No 63.55 62.46 5.20 6.18 N227T + F276A + N227T + F276A + Disulfide K308E K308E Bridge A49W + S186R + A49W + S186R + A269C + 68.75 68.64 N227T + A269C + N227T + F276A + Y282C F276A + Y282C + K308E K308E A49W + S186R + A49W + S186R + No 59.72 54.84 4.88 9.24 N227T + K308E N227T + K308E Disulfide Bridge A49W + S186R + A49W + S186R + A269C + 64.60 64.08 N227T + A269C + N227T + K308E Y282C Y282C + K308E A49W + Y124R + A49W + Y124R + No 63.30 62.69 5.25 5.54 N227T + F276A + N227T + F276A + Disulfide K308E K308E Bridge A49W + Y124R + A49W + Y124R + A269C + 68.55 68.23 N227T + A269C + N227T + F276A + Y282C F276A + Y282C + K308E K308E A49W + Y124R + A49W + Y124R + No 61.89 57.85 2.76 6.66 S186R + N227T + S186R + N227T + Disulfide F276A F276A Bridge A49W + Y124R + A49W + Y124R + A269C + 64.65 64.51 S186R + N227T + S186R + N227T + Y282C A269C + F276A + F276A Y282C A49W + Y124R + A49W + Y124R + No 64.61 62.60 4.17 5.86 S186R + N227T + S186R + N227T + Disulfide F276A + K308E F276A + K308E Bridge A49W + Y124R + A49W + Y124R + A269C + 68.78 68.46 S186R + N227T + S186R + N227T + Y282C A269C + F276A + F276A + K308E Y282C + K308E A49W + Y124R + A49W + Y124R + No 61.72 56.68 4.02 7.91 S186R + N227T + S186R + N227T + Disulfide K308E K308E Bridge A49W + Y124R + A49W + Y124R + A269C + 65.74 64.59 S186R + N227T + S186R + N227T + Y282C A269C + Y282C + K308E K308E N227T + F276A + N227T + F276A + No 63.38 62.25 4.86 5.91 K308E K308E Disulfide Bridge N227T + A269C + N227T + F276A + A269C + 68.24 68.16 F276A + Y282C + K308E Y282C K308E S186R + F276A + S186R + F276A + No 59.16 58.33 4.88 4.81 K308E K308E Disulfide Bridge S186R + A269C + S186R + F276A + A269C + 64.04 63.14 F276A + Y282C + K308E Y282C K308E S186R + N227T + S186R + N227T + No 59.58 57.18 5.17 6.47 F276A F276A Disulfide Bridge S186R + N227T + S186R + N227T + A269C + 64.75 63.65 A269C + F276A + F276A Y282C Y282C S186R + N227T + S186R + N227T + A269C + 68.74 68.43 ND ND A269C + F276A + F276A + K308E Y282C Y282C + K308E S186R + N227T + S186R + N227T + No 58.87 54.49 5.55 8.88 K308E K308E Disulfide Bridge S186R + N227T + S186R + N227T + A269C + 64.42 63.37 A269C + Y282C + K308E Y282C K308E Y124R + S186R + Y124R + S186R + No 63.60 62.60 4.88 5.71 N227T + F276A + N227T + F276A + Disulfide K308E K308E Bridge Y124R + S186R + Y124R + S186R + A269C + 68.48 68.31 N227T + A269C + N227T + F276A + Y282C F276A + Y282C + K308E K308E
(1098) Table 9 shows, that addition of the disulfide bride A269C+Y282 increases the Tm (melting temperature) values both in a buffer system and in buffer supplemented with the detergent Model A (approx. 2-10° C.=Delta Tm) for all the tested variants (Base mutation +/−Disulfide bridge). A Tm increase are here used as evaluation for increased stability of the variants.