Methods and Compositions for the Treatment of Multiple Sclerosis
20220142967 · 2022-05-12
Inventors
Cpc classification
International classification
Abstract
Compositions and methods based on isosorbide esters for the treatment of multiple sclerosis.
Claims
1. A method of treating multiple sclerosis or the symptoms thereof said method comprising administering to the patient suffering therefrom a therapeutically effective amount of a compound according to Formula (I): ##STR00007## in which R.sub.1 and R.sub.2 are both —C(O)CH═CH—C(O)OMe or one of R.sub.1 and R.sub.2 is H and the other —C(O)CH═CH—C(O)OMe, wherein Me is methyl.
2. The method of claim 1 wherein the compound is of the formula (I)(a) ##STR00008##
3. The method of claim 1 wherein the compound is of the formula (I)(b) ##STR00009##
4. The method of claim 1 wherein the compound is isosorbide di-(methylfumarate).
5. The method of claim 1 wherein the compound is administered systemically.
6. The method of claim 1 wherein the compound is administered locally to one or more areas of a patient where manifestation of the symptoms of MS is found.
7. The method of claim 1 wherein the compound is also administered orally.
8. A method of treating multiple sclerosis or the symptoms thereof said method comprising administering to the patient suffering therefrom a therapeutically effective amount of composition comprising at least one compound according to Formula (I): ##STR00010## in which R.sub.1 and R.sub.2 are both —C(O)CH═CH—C(O)OMe or one of R.sub.1 and R.sub.2 is H and the other —C(O)CH═CH—C(O)OMe, wherein Me is methyl and a pharmaceutically acceptable vehicle.
9. The method of claim 8 wherein the compound is of the formula (I)(a) ##STR00011##
10. The method of claim 8 wherein the compound is of the formula (I)(b) ##STR00012##
11. The method of claim 8 wherein the compound is isosorbide di-(methylfumarate).
12. The method of claim 8 wherein the at least one compound of formula (I) comprises a combination of isosorbide di-(methylfumarate) and isosorbide mono-(methyfumarate).
13. The method of claim 8 wherein the composition further comprises at least one other pharmaceutical active.
14. The method of claim 13 wherein the other pharmaceutical active is one that reduces, ameliorates, inhibits or otherwise addresses or treats MS symptoms and/or conditions associated with MS and its concurrent diseases/maladies.
15. The method of claim 13 wherein the other pharmaceutical active is selected from Fingolimod or Teriflunomide or a combination thereof.
16. The method of claim 8 wherein the composition is administered systemically.
17. The method of claim 8 wherein the composition is administered locally to one or more areas of a patient where manifestation of the symptoms of MS is found.
18. The method of claim 8 wherein the composition is administered orally.
19. A composition for use in the treatment of multiple sclerosis or the symptoms thereof said composition comprising administering to the patient suffering therefrom a therapeutically effective amount of composition comprising at least one compound according to Formula (I): ##STR00013## in which R.sub.1 and R.sub.2 are both —C(O)CH═CH—C(O)OMe or one of R.sub.1 and R.sub.2 is H and the other —C(O)CH═CH—C(O)OMe, wherein Me is methyl, and at least one other pharmaceutical active that reduces, ameliorates, inhibits or otherwise addresses or treats MS symptoms and/or conditions associated with MS and its concurrent diseases/maladies, and a pharmaceutically acceptable vehicle.
20. The composition of claim 19 wherein the other pharmaceutical active is selected from Fingolimod or Teriflunomide or a combination thereof.
21. The composition of claim 19 wherein the compound is of the formula (I)(a) ##STR00014##
22. The composition of claim 19 wherein the compound is of the formula (I)(b) ##STR00015##
23. The composition of claim 19 wherein the compound is isosorbide di-(methylfumarate).
Description
DETAILED DESCRIPTION OF THE FIGURES
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[0030] The file of this patent contains a least one drawing executed in color. Copies of this patent with color drawings will be provided by the United States Patent and Trademark Office upon request and payment of the necessary fee.
DETAILED DESCRIPTION OF THE INVENTION
[0031] As used in the present specification, the following terms shall have the meanings as presented:
[0032] “Patient” refers to a mammal, for example, a human.
[0033] “Pharmaceutically acceptable” means that the subject of this descriptor has been approved or is otherwise approvable by a regulatory agency of a government or governmental or is listed in the U.S. Pharmacopoeia or other generally recognized pharmacopoeia for use in animals, and more particularly in humans.
[0034] “Pharmaceutically acceptable vehicle” refers to a pharmaceutically acceptable diluent, a pharmaceutically acceptable adjuvant, a pharmaceutically acceptable excipient, a pharmaceutically acceptable carrier, or a combination of any of the foregoing with which a pharmacological active agent, including the compounds provided by the present disclosure, can be administered to a patient, which does not destroy or have a marked adverse effect on the pharmacological activity of the therein contained pharmacological active agent or metabolite thereof and which is non-toxic when administered in doses sufficient to provide a therapeutically effective amount of the pharmacological active agent or metabolite thereof.
[0035] “Pharmaceutical composition” refers to a composition comprising a pharmaceutically acceptable vehicle and a pharmacological active agent or metabolite, especially, in the case of pharmaceutical compositions claimed by the present application, pharmacological actives of Formula (I).
[0036] “Preventing” or “prevention” of any disease refers to reducing the risk of acquiring the disease, as through the use of a pharmacological active agent as a vaccine.
[0037] “Treating” or “treatment” of any disease refers to reversing, alleviating, arresting, inhibiting, or ameliorating a disease or at least one of the clinical symptoms of a disease, inhibiting the progress of a disease or at least one of the clinical symptoms of the disease as well as delaying the onset of a disease or at least one or more symptoms thereof in a patient who is predisposed to a disease, especially as evidenced by genetic testing, even though that patient does not yet experience or display symptoms of the disease. In following, treating or treatment also refers to inhibiting a disease, either physically, (e.g., stabilization of a discernible symptom), physiologically, (e.g., stabilization of a physical parameter), or both, and to inhibiting at least one physical parameter that may or may not be discernible to the patient.
[0038] “Improve” or “improvement” is used to convey the fact that the pharmacological active agent has manifested or effected changes, most notably beneficial changes, in either the characteristics and/or the physical attributes of the tissue to which it is being provided, applied or administered. These terms are also used to indicate that the symptoms or physical characteristics associated with the diseased state are diminished, reduced or eliminated.
[0039] “inhibiting” generally refers to delaying the onset of the symptoms, delaying or stopping the progression of the symptoms, alleviating the symptoms, or eliminating the disease, condition or disorder.
[0040] “Optional” or “optionally” means that the subsequently described subject, event or circumstance is not required or a necessary consequence, and that the description includes instances where the event occurs and instances where it does not and/or when the subject is present and when it is not present.
[0041] “Therapeutically effective amount” refers to the amount of a compound or composition that, when administered to a patient for treating a disease, or at least one of the clinical symptoms of a disease, is sufficient to effect such treatment of the disease or symptom thereof. The “therapeutically effective amount” varies depending, for example, on the compound or composition, the disease and/or symptoms of the disease, the severity of the disease and/or symptoms of the disease, the age, weight, and/or health of the patient to be treated, and the judgment of the prescribing physician. An appropriate amount of any given compound or composition can be ascertained by those skilled in the art and/or is capable of determination by routine experimentation.
[0042] “Therapeutically effective dose” refers to a dose that provides effective treatment of a disease in a patient. A therapeutically effective dose varies from compound/composition to compound/composition and/or from patient to patient, and depends upon factors such as the condition of the patient and the route of delivery as well as those described in the preceding definition of therapeutically effective amount. A therapeutically effective dose can be determined in accordance with routine pharmacological procedures known to those skilled in the art.
[0043] Erring on the side of caution and in an effort to avoid having overlooked or inadvertently omitted certain descriptive matter, particularly complementary and supplementary descriptive matter, Applicant hereby states and affirms that the technical publications as well as the patent and patent application publications mentioned herein are all incorporated herein in their entirety by this reference. Indeed, for example, while Applicant could present page after page of description of suitable pharmaceutically acceptable vehicles, such would not be productive as the same are well known and well recognized by those skilled in the art and those that come into being subsequent to the filing of this application will readily be appreciated as suitable as well. The same holds true for many other potential constituents, both active and non-active, that are employed in pharmaceutical compositions made in accordance with the present teachings.
[0044] In accordance with a first aspect of the present disclosure there are provided novel compositions for the treatment of multiple sclerosis and related diseases, comprising one or more compounds according to the general Formula (I):
##STR00003##
in which R.sub.1 and R.sub.2 are both —C(O)CH═CH—COOMe or one of R.sub.1 and R.sub.2 are H and the other —C(O)CH═CH—COOMe wherein Me is methyl, especially the mono- and di-fumarate esters, formulae (I)(a) and (I)(b), respectively, whose structure are as follows:
##STR00004##
[0045] Especially preferred are isosorbide di-(methyfumarate) (IDMF) and isosorbide mono-(methylfumarate) (IMMF). It is to be appreciated that in the above Formula (I), the structural orientation of the —OR.sub.1 and —OR.sub.2 groups can be in an endo orientation (an isomannide), an exo orientation (an isoidide) or one can be endo and the other exo (an isosorbide). Owing to their structure, the isomannide and isoidide compounds are both symmetrical molecules; whereas, because isosorbide has one endo and one exo group, mono-acylation gives rise to two different non-equivalent ester products, namely a 2-ester or a 5-ester. Generally speaking, it has been found that these compounds have the characteristics of bis secondary alcohols attached to two cis-fused tetrahydrofuran rings and as such possess the properties of both diols and ethers. The preferred compound according to Formula (I) is the isosorbide di-(methyl fumarate) (IDMF) whose structure (IV) is given below:
##STR00005##
[0046] The compounds of Formula (I) are derived from dianhydrohexitols, which are well documented by-products of the starch industry obtained by dehydration of D-hexitols, which are made by a simple reduction of hexose sugars. About 650,000 tons of dianhydrohexitols are produced annually worldwide. These chiral biomass-derived products exist as three main isomers (isosorbide (V), isomannide (VI), and isoidide (VII)), depending on the configuration of the two hydroxyl functions (derived from D-glucose, D-mannose, and L-fructose, respectively). Isosorbide, which is produced from glucose via sorbitol, is the most widely available dianhydrohexitol.
##STR00006##
[0047] These dianhydrohexitols compounds, as well as the lower (C1-C4) mono- and di-alkyl ethers thereof, and the mono and di-nitrates thereof, are well known and already used in various medical, pharmaceutical and health and beauty applications. The unsubstituted and lower alkyl substituted isohexides are very soluble in water and biologically harmless. The lower alkyl ethers and the unsubstituted compounds have been used as carriers in a number of skin care products to aid in the transport of other active ingredients through the skin membrane. The lower alkyl ethers have also found utility in dentifrices, aiding in the removal of plaque due to their osmotic properties. Isosorbide dinitrate and isosorbide mononitrate have been used to treat angina pectoris. Like other nitric oxide donors, these drugs lower portal pressure by vasodilation and decreasing cardiac output.
[0048] Certain higher alkyl (C6 and higher) mono- and/or di-esters of the dianhydrohexitols have drawn significant scientific and commercial interest. For example, isosorbide di-caprylate (HydraSynol® DOI) and isosorbide di-(linoleate/oleate) (HydraSynol® IDL), both from Sytheon Ltd., have recently been shown to have skin hydration and barrier building properties and are used in skin care and treatment products (See e.g. Chaudhuri U.S. Pat. No. 8,496,917 and Chaudhuri, U.S. Ser. No. 15/277,990, filed Sep. 22, 2016, respectively). Similarly, compounds of Formula (I) have also been shown to be effective in the treatment of psoriasis (See Chaudhuri et. al. US Patent Publication 2016/0279092).
[0049] In accordance with the present teachings, compounds according to Formula (I) have, surprisingly, been found to be very effective in the treatment of multiple sclerosis and related diseases and may also be suitable for use in the prevention of MS. In this respect, IDMF and IMMF have been found to be multi-targeted/targeting compounds which simultaneously affect various factors/conditions of MS. Additionally, IDMF is devoid of skin sensitization and flushing: issues plaguing DMF as an effective MS treatment. Although these compounds can be used as is, they are preferably formulated with or into suitable pharmaceutically acceptable vehicles and/or pharmaceutical compositions: vehicles and/or compositions that are specially formulated for these compounds or vehicles and compositions that are well known and/or used for administering pharmacological active agents. Similarly, these compounds and the compositions containing the same can be administered by any suitable means or method depending upon the nature of the composition itself and the intended target in the patient. For example, the compounds of the present teaching can be administered in accordance with various methods of application, e.g., oral, intramuscular, intravenous, transdermal, formulations, as the sole active or as one of several actives, which act independently or in conjunction with one another as well as in formulations that allow for sustained/timed release dosing.
[0050] As noted above, the compounds of Formula (i), especially the dianhydrohexitol di-(methylfumarate), most especially isosorbide di-(methylfumarate) (IDMF), has surprisingly been found to provide a marked effect in ameliorating, reducing, delaying and/or reversing or otherwise treating the effects and/or manifestation of MS as well as other disease manifesting similar symptoms and/or sharing common gene expression profiles, at least with respect to those genes that appear to be disease related. While not intending to be bound by theory or mechanisms, it is believed that these compounds, especially IDMF, is capable of modulating key genes/proteins related to NRF2 pathway in MS involving numerous immune axes, particularly various arms of the T-lymphocyte axis, and elevated oxidative stress, including impaired antioxidant responses.
[0051] While the compounds of Formula (I) are effective individually, they can be, and are preferably and/or beneficially, used in combination with each other, e.g., combinations of two or more compounds of Formula (I), and/or in combination with other pharmacologically active compounds, particularly compounds that similarly reduce, ameliorate, inhibit or otherwise address or treat symptoms and/or conditions associated with MS as well as related diseases, specifically in association with other pharmacological active agents use to treat MS and/or other diseases often associated with or concurrently manifesting with MS. Such combinations of compounds and actives provide further surprising results in terms of their pharmacological activity, especially with respect to the treatment of MS and other diseases which manifest and/or have common effects on gene expression profiles. Such other pharmaceutical actives can be selected to treat the same disease or symptoms as the compounds of Formula (I) or a different disease or symptom. Alternate drugs useful for treating MS which can be combined with the compounds of Formula (I) or into which compounds of Formula (I) can be incorporated include, but are not limited to, Fingolimod, Teriflunomide, Dimethyl fumarate (DMF). Most especially, as noted above, the compounds of Formula (I) are incorporated into various pharmaceutical compositions for administration to a patient. These additional actives can be combined together and the combination of actives administered as a single pharmaceutical composition or administered independently, in concurrent or sequentially administered pharmaceutical compositions.
[0052] Thus, in accordance with yet another aspect of the teaching of the present disclosure there are provided pharmaceutical compositions and methods of treatment comprising a combination of two or more compounds according to Formula (I) as well as combinations of at least one compound of Formula (I) and one or more other suitable pharmaceutical active. Such combinations of active compounds and their application or administration is found to have improved and/or synergistic performance, particularly with respect to the treatment of MS and diseases which manifest similar symptoms and/or common gene expression profiles.
[0053] The pharmaceutical compositions provided by the present disclosure can be formulated in a unit dosage form. A unit dosage form refers to a physically discrete unit suitable as a unitary dose for patients undergoing treatment, with each unit containing a predetermined quantity of a compound of Formula (I) calculated to produce an intended therapeutic effect. A unit dosage form can be for a single daily dose, for administration 2 times per day, or one of multiple daily doses, e.g., 3 or more times per day. When multiple daily doses are used, a unit dosage form can be the same or different for each dose. One or more dosage forms typically comprise a dose, which can be administered to a patient at a single point in time or during a time interval.
[0054] The pharmaceutical compositions comprising a compound of Formula (I) can be formulated for immediate release or for delayed or controlled release. In this latter regard, certain embodiments, e.g., an orally administered product, can be adapted for controlled release. Controlled delivery technologies can improve the absorption of a drug in a particular region, or regions, of the gastrointestinal tract. Controlled drug delivery systems are designed to deliver a drug in such a way that the drug level is maintained within a therapeutically effective window and effective and safe blood levels are maintained for a period as long as the system continues to deliver the drug with a particular release profile in the gastrointestinal tract. Controlled drug delivery typically and preferably produces substantially constant blood levels of a drug over a period of time as compared to fluctuations observed with immediate release dosage forms. For some drugs, maintaining a constant blood and tissue concentration of the drug throughout the course of therapy is the most desirable mode of treatment as immediate release of drugs oftentimes causes blood levels to peak above that level required to elicit a desired response. This results in waste of the drug and/or may cause or exacerbate toxic side effects. In contrast, the controlled delivery of a drug can result in optimum therapy; not only reducing the frequency of dosing, but also reducing the severity of side effects. Examples of controlled release dosage forms include dissolution controlled systems, diffusion controlled systems, ion exchange resins, osmotically controlled systems, erodable matrix systems, pH independent formulations, and gastric retention systems.
[0055] As noted, the compounds of Formula (I), more appropriately, the pharmaceutical compositions comprising compounds of Formula (I), can be administered through any conventional method. The specific mode of application or administration is, in part, dependent upon the form of the pharmaceutical composition, the primary purpose or target of its application (e.g., the application may be oral if intending to address the disease generally or topically or injection if intending to address primarily a topical symptom or location of a symptom of the disease. Suitable modes of administration include, for example, intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intranasal, epidural, oral, sublingual, intracerebral, intravaginal, transdermal, rectal, or inhalation. Especially preferred modes of administration are oral, or those methods that involve absorption through epithelial or mucous linings (e.g., oral mucosa, rectal, and intestinal mucosa, etc.). Furthermore, again, depending in part upon the form and primary purpose or target of the administration, the pharmaceutical compositions of the present disclosure can be administered systemically and/or locally. For example, hands, feet and legs are often a primary target of MS manifestation; hence, one may localize treatment to the hands, feet and/or legs to optimize dosing to those areas. Additionally, one may employ both systemic and local treatment to address MS systemically while concurrently localizing a stronger effect locally. Finally, the form of the pharmaceutical composition and its delivery system varies depending upon the parameters already noted. For example, orally administered pharmaceutical compositions of the present teaching can be in encapsulated form, e.g., encapsulated in liposomes, or as microparticles, microcapsules, capsules, etc.
[0056] Again as noted above, the compounds of Formula (I) can be used as is, i.e., as 100% of the composition to be applied; however, the compounds of Formula (I) are preferably incorporated into a pharmaceutical composition in which the compound(s) of formula account for from about 0.01 to about 99 weight percent of the pharmaceutical composition. Preferably, the compounds of Formula (I) will comprise from about 0.5 to about 30 wt %, more preferably from about 0.5 to about 20 wt %, most preferably from about 1.0 to about 10 wt % of the pharmaceutical composition. Another factor playing into the concentration of the compounds of Formula (I) in the pharmaceutical composition is the dose or rate of application of the compounds to the patient. Obviously, dosing itself depends upon a number of factors including the concentration and/or purity of the compounds of Formula (I), the efficacy thereof, the individual to whom the pharmaceutical is to be administered, the mode of administration, the form in which the pharmaceutical composition is to be administered, the disease or symptom to be addressed, etc.
[0057] The foregoing factors as well as the application thereof in formulating the compositions of the present teaching are all as well known in the art whereby the final or actual concentration in the pharmaceutical composition and/or the dose can readily be determined based up simple dose-response testing and the like. For example, an appropriate oral dosage for a particular pharmaceutical composition containing one or more compounds of formula (I) will depend, at least in part, on the gastrointestinal absorption properties of the compound, the stability of the compound in the gastrointestinal tract, the pharmacokinetics of the compound and the intended therapeutic profile.
[0058] An appropriate controlled release oral dosage and ultimate form of a pharmaceutical composition containing a particular compound of Formula (I) will also depend upon a number of factors. For example, gastric retention oral dosage forms may be appropriate for compounds absorbed primarily from the upper gastrointestinal tract, and sustained release oral dosage forms may be appropriate for compounds absorbed primarily from the lower gastrointestinal tract. Again, it is to be expected that certain compounds are absorbed primarily from the small intestine whereas others are absorbed primarily through the large intestine. It is also to be appreciated that while it is generally accepted that compounds traverse the length of the small intestine in about 3 to 5 hours, there are compounds that are not easily absorbed by the small intestine or that do not dissolve readily. Thus, in these instances, the window for active agent absorption in the small intestine may be too short to provide a desired therapeutic effect in which case large intestinal absorption must be channeled and/or alternate routes of administration pursued.
[0059] Generally speaking, an appropriate dose of a compound of Formula (I), or pharmaceutical composition comprising a compound of Formula (I), can be determined according to any one of several well-established protocols including in-vitro and/or in-vivo assays and/or model studies as well as clinical trials. For example, animal studies involving mice, rats, dogs, and/or monkeys can be used to determine an appropriate dose of a pharmaceutical compound. Results from animal studies are typically extrapolated to determine appropriate doses for use in other species, such as for example, humans.
[0060] As noted above, the compositions according to the present teaching can be designed for immediate infusion or application of the actives to the body or site of the symptom to be treated. However, it is also recognized that in certain instances the pharmaceutical compositions provided by the present disclosure can be, and are preferably, adapted to provide sustained or timed release of a compound of Formula (I): this is especially so and desirable for oral administration. Sustained release oral dosage forms are used to release drugs over a prolonged time period and are useful when it is desired that a drug or drug form be delivered to the lower gastrointestinal tract. Sustained release oral dosage forms include any oral dosage form that maintains therapeutic concentrations of a drug in a biological fluid such as the plasma, blood, cerebrospinal fluid, or in a tissue or organ for a prolonged time period. Sustained release oral dosage forms include diffusion-controlled systems such as reservoir devices and matrix devices, dissolution-controlled systems, osmotic systems, and erosion-controlled systems. Sustained release oral dosage forms and methods of preparing the same are well known in the art.
[0061] Following on the foregoing, the amount of a compound of Formula (I) contained in a dose depends upon, among other factors, the route of administration and whether the disease in a patient is effectively treated by acute, chronic, or a combination of acute and chronic administration. In any event, the administered dose is typically less than a toxic dose: though it may have significant adverse health effects, provided that the desired beneficial effect is also attained. Toxicity of the compositions described herein can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., by determining the LD.sub.50 (the dose lethal to 50% of the population) or the LD.sub.100 (the dose lethal to 100% of the population). The dose ratio between toxic and therapeutic effect is the therapeutic index. In certain embodiments, a compound or metabolite thereof may exhibit a high therapeutic index. The data obtained from these cell culture assays and animal studies can be used in formulating a dosage range that is not toxic for use in humans. A dose of a compound of Formula (I) is typically set within a range of circulating concentrations in for example the blood, plasma, or central nervous system, that include the effective dose and that exhibits little or no toxicity. A dose can vary within this range depending upon the dosage form employed and the route of administration utilized. In certain embodiments, an escalating dose can be administered.
[0062] Where additional pharmacological actives may be and preferably are also present in the compositions according to the present teaching, the amount by which they are present and/or the dosage amount will typically be consistent with their conventional concentration and rates of application. For example, such other actives will be present in an amount of from about 0.5 to about 30 wt %, more preferably from about 0.5 to about 20 wt %, most preferably from about 1.0 to about 10 wt % of the pharmaceutical composition. Of course, as noted, the combination of these other pharmacological actives with one or more compounds of Formula (I) also provide enhanced performance and/or synergy whereby the amounts of each and/or the dose of each is generally less than required for the use of the individual active compounds on their own.
[0063] Without further elaboration, it is believed that one skilled in the art can, using the preceding description, utilize the present invention to its fullest extent. The following preferred specific embodiments are, therefore, to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.
EXAMPLES
[0064] Experimental I
[0065] An initial series of experiments was performed to demonstrate the effect and comparative effect of IDMF and DMF in various MS related pathways.
Example 1
[0066] A study was conducted using microarrays to evaluate the effects of IDMF on genome-wide expression in cultured human keratinocytes (KCs) in an effort to demonstrate the effect of IDMF on gene expression of genes associated with multiple sclerosis (MS) and, in particular, to demonstrate that IDMF induces the transcription of genes associated with nuclear factor (erythroid-derived 2)-like 2 (NFE2L2/NRF2), factors known to be associated with MS. RNA sample processing and microarray hybridizations were performed using the Affymetrix Human Genome U133 Plus 2.0 array platform. DNA microarray samples evaluated included IDMF- and vehicle-treated KCs (n=2 replicates per group). Samples were normalized using the robust multichip average (RMA) algorithm. Identification of probe sets with detectable expression was performed using the MAS 5.0 method, which identifies probe sets with above-background expression based upon the Wilcoxon signed rank test. To limit redundancy in downstream analyses, a single probe set was chosen to represent each protein-coding human gene featured on the microarray platform. This representative was chosen as the probe set with highest expression for each protein-coding human gene. These probe sets were then additionally filtered to include only those with detectable expression in at least 1 of the 4 samples (P<0.05, Wilcoxon signed rank test). Overall, this yielded a total of 12330 protein-coding genes that were included in analyses, with each gene represented by a single representative probe set. Bayesian linear models and moderated t-statistics were then used to evaluate differential expression for each of the 12330 genes (IDMF vs. CTL treatment; R package: limma). To control the false discovery rate (FDR), raw p-values generated from linear model analyses were adjusted using the Benjamini-Hochberg method.
[0067] MS Gene Association
[0068] Genes associated with multiple sclerosis were identified based upon four established database sources: (1) the NHGRI-EBI genome-wide association (GWA) study catalogue, (2) the Disease Ontology (DO) database, (3) the DisGeNET database, and (4) the Medical Subject Heading (MESH) database. While there is some overlap between the various databases, the variation in identified genes from one database to the other reflects the fact that the science is still developing and has uncertainties. Nevertheless, as discussed below, efforts were undertaken to hone in on genes common to multiple databases.
[0069] MS-associated genes identified from each of the four sources were evaluated to assess whether they exhibited atypical responses to IDMF, either exhibiting a trend towards increased or decreased expression as shown in the histograms of
[0070] Of the 175 MS-associated genes identified as common to at least two of the four databases (
[0071] As indicated in the histograms of
[0072] Nuclear Factor (Erythroid-Derived 2)-Like 2 (NFE2L2/NRF2) Study
[0073] As noted in the background section above, the transcription factor nuclear factor (erythroid-derived 2)-like 2 (NFE2L2/NRF2) has previously been associated with multiple sclerosis. In this study, IDMF-regulated genes were evaluated to determine the extent to which IDMF led to the enrichment of NFE2L2/NRF2 binding sites in promoter-proximal regions. For these analyses, a NFE2L2/NRF2 binding site was obtained from the JASPAR database (JASPAR ID: MA0150). This binding site partially matches the consensus sequence 5-[A/G]TGAC—AGCA-3: additional information can be obtained from http://jaspar.genereg.net/cgi-bin/jaspar_db.pl?ID=MA0150.1&rm=present&collection=CORE. Tests for enrichment were performed by scanning regions 1 kb, 2 kb or 5 kb upstream of protein-coding genes, including either all sequence (conserved=No) or only sequences conserved among mammalian species (conserved=Yes). A positive Z statistic indicates a trend towards increased NFE2L2 (NRF2) binding sites in sequences upstream of IDMF-increased Database Essential Genes (DEGs), as compared to all other expressed genes). P-values indicate the significance of the reported Z statistic (semiparametric generalized additive logistic models). Predicted targets include IDMF-increased DEGs with the NFE2L2 (NRF2) binding site identified within the scanned upstream region.
[0074] Results, as shown in Table 2, demonstrate that this NFE2L2/NRF2 binding site is significantly enriched in regions upstream of IDMF-increased DEGs (FDR<0.10 with FC>1.50). The enrichment tended to increase when sequences closer to the transcription start site were evaluated, and when only sequence regions conserved among mammalian species were analyzed. These trends suggest that the NFE2L2/NRF2 binding sites act as functional mediators of transcription for IDMF-increased genes.
[0075] The IDMF-increased genes with the NFE2L2/NRF2 binding site in close proximity (<1 kb) to their transcription start site include GSR, AIFM2, PIR, NQO1, CYP4F3, BEX5, GCLM, GPX2, and CYP4F11 (Table 2). The analysis was repeated with respect to IDMF-decreased genes (FDR<0.10 with FC<0.67), but in contrast such genes did not appear to show increased frequency of the NFE2L2/NRF2 binding site in their upstream region. Nevertheless, it should be noted that other binding sites were also identified showing a stronger degree of enrichment. For IDMF-increased genes (FDR<0.10 with FC>1.50), the strongest enrichment was observed for binding sites recognized by IRF1 (TWTTCA), LSM6 (ATGNARA) and HOXB5 (TAATTR). For IDMF-decreased genes (FDR<0.10 with FC<0.67), the strongest enrichment was observed for binding sites recognized by NF-kappaB (GGRAWTYCCC), MCTP2 (TTCCY) and FOXP4 (MTTTYCC). Although not a focal point of this analysis, it was noted that IDMF-decreased genes were especially and strongly enriched for NF-kappaB biding sites in their upstream regions (P=1.16E-08). IDMF-decreased genes associated with this binding site include IL23A, IL36G, ICAM1, and S100A7 and as associated with anti-inflammatory mechanisms which are believed to be especially beneficial in multiple disease states characterized by autoimmunity and/or inflammation: issues associated with MS. Indeed, as noted previously, it has been proposed that NF-kappaB provides a target for treatment of multiple sclerosis (PMID: 19128210; PMID: 24007818).
TABLE-US-00002 TABLE 2 Enrichment of NFE2L2 (NRF2) binding sites Region Conserved Z Statistic P-value Predicted Targets 1 kb No 4.74 2.11E−06 GSR, AIFM2, PIR, NQO1, CYP4F3, BEX5, GCLM, GPX2, CYP4F11 2 kb No 3.37 0.00074 AIFM2, NQO1, NAP1L2, GCLM, PIR, BEX5, CLMP, CYP4F3, GPX2, CYP4F11 5 kb No 2.11 0.035 NAP1L2, NQO1, CYP4F3, AIFM2, BEX5, PIR, CLMP, GCLC, CYP4F11, CNNM4, GPX2, SPP1, COL11A1 1 kb Yes 5.91 3.39E−09 GSR, AIFM2, GPX2, BEX5, GCLM, PIR, NQO1, CYP4F3, CYP4F11 2 kb Yes 3.21 0.0013 NQO1, NAP1L2, GCLM, GPX2, PIR, CYP4F3, CYP4F11 5 kb Yes 1.05 0.293 NAP1L2, NQO1, GCLC, GPX2, COL11A1, PIR
Example 2—IDMF/DMF
[0076] A separate assay study was conducted on cytokine-treated neonatal human epidermal keratinocytes (HEK) looking at the expression impact of IDMF and dimethyfumarate (DMF) on key pro-inflammatory and antioxidant genes. In preparation for the assay, HEK (p2; Cell Applications, San Diego) were cultured at optimal conditions [keratinocyte growth medium supplemented with KGM-Gold Bullet kit (Lonza, Switzerland)] to form a segmented monolayer. A cytokine mix of IL-17A/IL-22/TNFα (100 ng/ml; 100 ng/ml; 10 ng/ml; Antigenix, Huntington Station, N.Y.) was added and the cell cultures incubated for 24 h. Meanwhile the test formulations of IDMF and DMF were prepared by solubilizing each in DMSO to a concentration of 20 mg/ml and then diluting those solutions with water to a concentration of to 80 μg/ml. Thereafter, each test material was added to the cultures at 4 μg/ml (final concentration) in triplicate and the cultures incubated for a further 24 hours.
[0077] After the 24-hour incubation with the test materials, the cells were observed through a Nikon Eclipse TS100 inverted microscope. No gross morphological changes were recorded. RNA was then extracted and purified with NucleoSpin RNA II kit from Machery-Nagel, Bethlehem, Pa., using QiaCube robotic station (Qiagen, Valencia, Calif.). Purified total RNA was assessed at 260 nm and 280 nm with NanoDrop Lite (Thermo Fisher Scientific, Waltham, Mass.), pure samples with A.sub.260A.sub./280 and A.sub.260/A.sub.230 ratios of >1.7 were standardized and the expression of the genes of interest (Table 3) was measured by real-time quantitative PCR with BioRad iCycler iQ Detection System using custom-made PCR arrays (Qiagen, Valencia, Calif.; part #24caph12190), 5×All-In-One 1st Strand cDNA Synthesis Mix (Bioland Scientific, Paramount, Calif.) and 2×qPCR Master Mix (Bioland). Efficiency ΔΔCt method was used for quantification of results, after the normalization of gene expression to GAPDH/PPIA housekeeping gene. Genes were considered differentially expressed if the level of expression was reasonably high (less than 30 cycles to detect) and the modulation was >1.7. However, in noting the gene expressions attained, it is to be appreciated that given the difference in their molecular weights, when used at the same concentration (by wt) there is nearly 2.5 times as many moles of DMF as compared to IDMF. Even assuming IDMF fully metabolizes to MMF, there is still considerably more MMF from DMF as opposed to the same amount, based on weight, of IDMF. Hence, it is believed that had equivalent molar amounts of DMF and IDMF, based on available MMF, been used, the beneficial impact of IDMF would have been more apparent.
[0078] The results attained were as presented in Table 3. CXCL3, IL8 and PTGS2 all are genes associated with inflammation or pro-inflammatory effects. As noted in Table 3, DMF induced an upregulation in all three genes, indicative of an inflammatory inducing effect, whereas in the case of IDMF it is non-existent, if not of an anti-inflammatory effect, particularly with respect to CXCL3. Most significantly, IDMF had a markedly higher upregulation of NFE2L2 than DMF, even at the lower molar concentration. This is especially important with respect to the current claimed method since, as noted in the previous study, nuclear factor (erythroid-derived 2)-like 2, also known as NFE2L2 or Nrf2, is directly tied to MS treatment (Anna Hammer et al., The NRF2 pathway as potential biomarker for dimethyl fumarate treatment in multiple sclerosis, Ann Clin Transl Neurol, 5(6):668-676, 2018). Though not intended to be bound by theory, the mechanism of efficacy is believed to involve NFE2L2 (NRF2)-activated genes associated with glutathione metabolism and/or oxidative stress response. In this regard, NFE2L2 is a transcription factor that in humans is encoded by the NFE2L2 gene. It is a basic leucine zipper (bZIP) protein that regulates the expression of antioxidant proteins that protect against damage triggered by oxidative stress.
TABLE-US-00003 TABLE 3 Gene Expression IDMF v DMF DMF 4 μg/ml Detection IDMF 4 μg/ml Detection Gene name fold change level fold change level Comments CXCL3 1.7 OKAY −1.1 OKAY CXCL3 Chemokine (C-X-C motif) ligand 3 induces inflammation and is stimulated in keratinocytes by IL-17. IL8 2.3 OKAY 1.4 OKAY Interleukin-8 (IL-8) is a pro-inflammatory cytokine in the skin. PTGS2 1.7 OKAY 1.2 OKAY Prostaglandin-endoperoxide synthase (PTGS2), also known as cyclooxygenase 2 (COX2), is the key inducible enzyme in prostaglandin biosynthesis, and is involved in inflammation, contact dermatitis and mitogenesis. NFE2L2 1.8 OKAY 2.6 OKAY NFE2L2 gene codes for the nuclear factor (erythroid-derived 2)-like (NRF2) - the transcription factor that enables the expression of many antioxidant proteins.
Example 3
[0079] A further study was conducted to evaluate the effects of DMF and IDMF on gene expression in cultured astrocytes. Previously, Waller et al. 2016 (J Neuroimmunol 299:139-146) used laser capture microdissection (LCM) to isolate astrocytes from normal appearing white matter in MS patients and CTL subjects (GEO dataset GSE83670; PMID: 27125112). The study was performed using 3 treatments and 6 independent biological replicates (CTL=non-stimulated astrocytes, n=2; DMF=DMF-treated astrocytes, n=2; IDMF=IDMF-treated astrocytes, n=2). Comparison of DMF/IDMF expression responses to MS vs. CTL expression changes from the Waller at al. 2016 study allows us to evaluate whether DMF/IDMF expression responses oppose those occurring in MS patients for the same cell type.
[0080] cDNA sequencing was performed by the University of Michigan sequencing core facility (50 cycle single end Illumina HiSeq 2000). The FASTX-Toolkit was used to filter reads by removing low quality sequences and initial quality assessments were performed using FastQC. Quality-filtered reads were mapped to the human genome (hg38/GRCh38, UCSC) using tophat2 with default settings, except multi-mapping of reads to multiple genome locations was disallowed (settings: -g 1 —no-coverage-search). The hg38/GRCh38 genome is the most recent release available from UCSC. HTSeq was used to tabulate the number of reads mapping to each genome feature and Cufflinks was used to calculate FPKM estimates and associated confidence intervals. RSeQC was used to assess quality of mapping and calculate the percentage of mapped reads. The negative binomial model (edgeR) was used to evaluate differential expression, with read counts normalized using the weighted trimmed mean of M-values method, and dispersions estimated using the Cox-Reid (CR)-adjusted likelihood approach. Differential expression analyses were performed using genes with detectable expression in at least 1 of 4 samples involved in a given comparison. A gene was considered to have detectable expression in a sample if the count per million mapped reads (cpm) estimate was greater than 0.20 and if the lower bound of the FPKM 95% confidence interval was greater than zero.
[0081]
[0082] Based on the results presented in
[0083] In contrast, genome-wide expression responses to IDMF were negatively correlated with those observed in MS patients (scatterplot (D)), indicating that IDMF tended to oppose expression shifts seen with MS. 35.08% of protein-coding genes were increased by IDMF (FC>1.00) but decreased in MS patients (FC<1.00). This percentage was significantly larger-than-expected (P<0.05, Fisher's exact test. Genes increased by IDMF at a P<0.05 threshold did not significantly overlap with genes decreased by MS at the same threshold (P=0.26; gene set overlap (E)). However, genes decreased by IDMF at a P<0.05 threshold did overlap significantly with genes increased by MS at the same threshold (P=4.8e-09; gene set overlap (F)). Overall, 82 genes were decreased by IDMF (P<0.05) and increased in MS (P<0.05), including IGFBP5, CD74 and UHRF1 (histogram (G)). Many of the IDMF-decreased and MS-increased genes were associated with translation and targeting of proteins to the endoplasmic reticulum. For example, GO BP terms associated with such genes included SRP-dependent cotranslational protein targeting to membrane, establishment of protein localization to endoplasmic reticulum, and nuclear-transcribed mRNA catabolic process (nonsense-mediated decay). These results are indicative of a marked and surprising benefit from the use of IDMF in the treatment of MS.
Example 4
[0084] In light of the recent studies regarding the neuroprotectant effect of DMF through NRF2 activation in the treatment of multiple sclerosis as shown by Gopal, S., et. al., “Evidence of activation of the Nrf2 pathway in multiple sclerosis patients treated with delayed-release dimethyl fumarate in the Phase 3 DEFINE and CONFIRM studies,” Mult Scler. 2017 December; 23(14):1875-1883. doi: 10.1177/1352458517690617. Epub 2017 Feb. 3 and Wang, Q., et. al. “Dimethyl Fumarate Protects Neural Stem/Progenitor Cells and Neurons from Oxidative Damage through Nrf2-ERK1/2 MAPK Pathway,” Int J Mol Sci, 2015 Jume 17:16(6):13885, a further study was conducted using the Cignal ARE Reporter Assay Kit to measure the transcriptional activity of NRF2 (NF-E2-related factor 2) transcription factor.
[0085] Stock solutions of DMF and IDMF were prepared in DMSO immediately before adding to cell cultures, and tested at serial aqueous dilutions of 1 μg/ml and 10 μg/ml and compared to a negative control comprising the diluted solvent. The experiments were performed using HEK293 cells (Sigma, St. Louis, Mo.; cat. #85120602) seeded at 50,000 per well in a 96 well white opaque-wall tissue culture plates. Cells were transfected by mixing Cignal Reporter Assay firefly/renilla luciferase constructs (cat. # CCS-5020L; Qiagen) with Attractene Transfection Reagent (Qiagen) in Opti-MEM (ThermoFisher Scientific, Waltham, Mass.) supplemented with 1% MEM Non-essential amino acid solution without L-glutamine (Sigma; cat. #M7145). After medium change test materials were added and their effect on NRF2 induction was quantified 24 h later with the Dual-Luciferase Reporter Assay System (cat. #E1960; Promega, Madison, Wis.).
[0086] The ARE (antioxidant response element) reporter is a mixture of a Nrf2-responsive luciferase construct encoding the firefly luciferase reporter gene under the control of a minimal (m)CMV promoter and tandem repeats of the ARE transcriptional response element, and a constitutively expressing Renilla element (40:1), which acts as an internal control for normalizing transfection efficiencies and monitoring cell viability. The number of response elements and the intervening sequence between these response elements has been experimentally optimized to maximize the signal to noise ratio. Signal quantification was obtained with ThermoFisher Scientific Luminoskan Ascent Microplate Luminometer. The results are presented in Table 4.
TABLE-US-00004 TABLE 4 DMF and IDMF on NRF2-mediated luciferase gene expression Fold Change v. Standard error Sample Concentration water control (1) of mean DMF 1 μg/ml 1.38 ±0.03 10 μg/ml 1.81 ±0.05 IDMF 1 μg/ml 2.78 ±0.28 10 μg/ml 4.61 ±0.42
[0087] As evident from the results shown in Table 4, while DMF proved efficacious in enhancing NRF2-mediated luciferase expression, particularly at the higher concentration, IDMF provided a surprising and marked effect on NRF2-mediated luciferase expression at both concentrations. Furthermore, it is to be recalled that at these equal weights, DMF has approximately 0.5 moles of additional methyl fumarate for every mole of IDMF. Hence, at methyl fumarate molar equivalent ratios, i.e., 2 moles of DMF for every mole of IDMF, it is expected that the fold change difference between IDMF and DMF would have been significantly higher.
Example 5—A1 v A2 Marker Activation of IDMF Vs DMF
[0088] DMF and IDMF (2.5 μM each) were applied to human fetal astrocytes for 24 hrs, with water employed as the negative control. The treated cells were stimulated with H.sub.2O.sub.2 (50 μM) for 20 min and the RNA extracted 1 day later and sequenced using the Illumina HiSeq 2000 with an average of 39.3 million reads per sample. Reads were mapped to the GHch38/hg38 genome. 13,968 protein-coding genes had detectable expression on average among the 6 samples. Differentially expressed genes (DEGs) were identified using negative binomial models with FDR threshold of <0.10 and fold change (FC) criteria (FC>1.30 or FC<0.77). Gene set enrichment testing was performed using public database annotations. Motif enrichment with respect to DEG up-stream regions was characterized using semiparametric generalized additive logistics models. The overlap between the identified DEGs and MS-associated genes, i.e., genes from MS patients and genes linked to inflammatory (A1) and ischemic (A2) astrogliosis. In-vitro fluorescence assays were used to evaluate effects of DMF and IDMF enzyme activity, and RTPCR was used to evaluate a subset of expression changes identified by RNA-seq. among the overlapping genes.
[0089] Among the overlapping genes was ICAM1, which is associated with the initiation and progression of inflammation as well as the targeting of inflammation in the central nervous system (CNS). IDMF was found to downregulate ICAM1, whereas, no similar activity was found with DMF indicative of an antagonism of inflammation in the CNS with IDMF, but not DMF. A similar result was observed with genes encoding cell cycle proteins. More importantly, 163, 198, and 85 genes associated with pan-reactive astrogliosis (A1+A2), A1-polarization, and A2-polarization, respectively were identified and assessed: the genes most strongly associated with each pattern included LCN2, C2ORF88, and VASH1, respectively. Although the effect size was small, there was a significant trend in which DMF tended to increase expression of genes linked to A1-polarization, i.e, neurotoxic or pro-inflammatory phenotype but, in contrast, decreased expression of genes linked to A2-polarization, i.e., neuroprotective or anti-inflammatory phenotype. In sharp contrast and supporting its efficacy in treatment of MS. IDMF tended to increase the expression of genes linked to A2 polarization while decreasing genes linked to pan-reactive gliosis: again, indicative of beneficial impact or repair.
Example 6—IDMF v DMF Metabolism
[0090] IDMF and DMF were subjected to carboxylesterase hydrolysis. Specifically, a plurality of solutions of 1 mM each of IDMF and DMF were prepared by dissolving each in 2% DMSO+10% Ethylene Glycol in 20 mM HEPES pH 7.4. 1 mg/ml carboxylesterase 2 (CES2) was added to each solution and the solutions incubated at 37° C. for the time periods set forth in Table 5. A sample of each solution was removed at the time period and the reaction stopped with methanol and the samples evaluated and quantified for any remaining IDMF and DMF as well as, in the case of IDMF, IMMF, the metabolite intermediate, using an UV detector at 210 nm. Isosorbide was quantified using CAD. Mobile phase used for the study: 0.1% Formic acid in water; B: Methanol. Column: Luna C18 4.6 mm×100 mm, 5 u (Phenomenex). Flow rate: 1.0 ml/min.
[0091] The results are presented in Table 5. As seen in Table 5, all but about 17% of the DMF is hydrolyzed to MMF within thirty minutes and essentially all within 60 minutes. On the other hand, surprisingly, essentially all of the IDMF had hydrolyzed to MMF and IMMF within 15 minutes. Equally surprising is the finding that from that point forward, there was a slow hydrolysis of IMMF to MMF and isosorbide. As an orally administered pro-drug which undergoes carboxylesterase hydrolysis in the gastrointestinal tract, DMF rapidly and nearly completely converts to MMF. On the other hand, the surprising release pattern shown with IDMF provides for a quick infusion or release of MMF, but without an overloading of MMF as seen with DMF, and eliminates or greatly reduces the generation of methanol; both of which characteristics lessen the likelihood of GI distress and provide for improve tolerance in patients treated with the same. Additionally, since the IDMF is converted to IMMF first, the use of IDMF unexpectedly provides for MMF as well as a reservoir or supply of additional MMF in the form of IMMF, which slowly metabolizes to release the additional MMF: thereby providing for a longer release and continued elevated level of MMF in the body. Essentially, IDMF has the capability of providing the efficacy of multiple, spaced doses of DMF in a single administration as well as the ability to enable the use of lesser amounts to achieve a better result than with DMF.
TABLE-US-00005 TABLE 5 Time Isosorbide (minutes) DMF (mM) IDMF (mM) IMMF (mM) (mM) 0 1 1.027 0 0 15 — 0.072 0.540 0 30 0.166 ND 0.367 0.176 60 0.08 ND 0.289 0.234 120 0.014 ND 0.191 0.455
[0092] Experimental II
[0093] A subsequent series of experiments was performed to demonstrate the effect and comparative effect of IDMF and DRF and MMF, the active metabolite of the foregoing as well as DMF, in various MS related pathways.
[0094] Astrocyte Cell Cultures
[0095] Experiments were performed using human fetal astrocytes derived from cerebral cortex (cat. no. 882A-05f; Cell Applications, Sant Diego, Calif.). Cells were grown to subconfluence in human astrocyte growth medium (cat. no. 820-500, Cell Applications). Stock solutions of MMF, DRF and IDMF were prepared in DMSO (50 mM) and subsequent dilutions were made using distilled water. The experiments were performed using 12 replicate cultures, each of which was assigned to one of 4 experimental treatments (CTL, n=3; MMF, n=3, DRF, n=3; IDMF, n=3). Test materials were added to subconfluent cultures at a concentration of 2.5 μM and cells were incubated for 24 hours.
[0096] RNA Processing
[0097] RNA was extracted following the incubation period using the RNAeasy Mini Plus kit (Qiagen, Hilden, Germany) and robotic Qiacube Connect station (Qiagen). Samples were shipped on dry ice to the Thermo Fisher Microarray Research Services Laboratory (Santa Clara, Calif.) for transcriptome analysis. RNA quality analysis was performed upon sample receipt using the NanoDrop Lite (Thermo Fisher Scientific, Waltham, Mass.). All samples had 260/280 nm absorbance ratios higher than 1.90, consistent with high-purity RNA.
Example 7—MS-Associated Gene Impact with IDMF, MMF and DRF
[0098] In follow-up to the study of Example, 1 above, genes associated with multiple sclerosis were identified based upon seven established database sources: (1) the NHGRI-EBI genome-wide association (GWA) study catalogue, (2) the Disease Ontology (DO) database, (3) the DisGeNET (curated) database, (4) the DisGeNET (BeFree), (5) MalaCards, (6) the Medical Subject Heading (MESH) database and the KEGG Database. The impact on gene expression of IDMF, MMF and DRF on the key overlapping MS-associated genes was assessed. The genes for this study as well as the impact thereon is presented in the table in
Example 8—NRF Pathways
[0099] The mechanism of action of DMF is often understood in terms of its activation of the NFR2 pathways, specifically the NRF2-dependent and NRF2-independent pathways, with the former postulated to mediate a neuroprotective effect on CNS cell types and the latter affecting inhibition of neuroinflammation and with modulation of peripheral immune cells. Assays were performed to assess the impact of IDMF, DRF and MMF on these same pathways in an effort to assess the cytoprotective and anti-inflammatory/antioxidant effect of these fumarates. Plots of the impact of MMF and DRF on the NRF2 target genes are shown in an overlay relationship with bar charts of the impact of IDMF on the same genes are presented in
Example 9—NF-kB Pathways
[0100] A similar study was undertaken focusing on the NF-kB pathways, pathways inhibited by DMF. Plots of the impact of MMF and DRF on the NF-kB (RELA) target genes are shown in an overlay relationship with bar charts of the impact of IDMF on the same genes are presented in
Example 10—IFR Motif Enrichment
[0101] Genetic variants of IFR has been associated with MS and some MS treatments such as glatiramer acetate block cytokine-stimulated IRF1 up-regulation. An assessment was undertaken to assess the relative impact of fumarate esters, namely MMF, DRF and IDMF on IFR motif enrichment.
Example 11—Comparative RT-PCR Evaluation
[0102] The expression of selected genes was further evaluated using RT-PCR. cDNA was prepared using the AzuraQuant cDNA kit (Azura Genomics, Raynham, Mass.) and PCR reactions were performed using the BioRad iCycler iQ Detection System with Fast Green qPCR Master Mix—Fluor (Azura Genomics). PCR primers were purchased from Realtimeprimers (Elkins Park, Pa.). The ΔΔCt method [Livak K. J. and Schmittgen T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods (San Diego, Calif.) 25(4):402-408, 2001]. was used to estimate fold changes with cycle threshold values normalized based upon the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The specific genes tested and the results attained therewith are summarized in
[0103] TNF is a known inducer of NF-kB, as shown in
[0104] Most significantly, as shown in
Example 12—IDMF v DRF Metabolism
[0105] Similar to Example 6 above, the hydrolysis of IDMF was compared to that of DRF. Specifically, a plurality of solutions of 1 mM each of IDMF and DRF were prepared by dissolving each in 2% DMSO+10% Ethylene Glycol in 20 mM HEPES pH 7.4. 20 μl of 1 mg/ml carboxylesterase 2 (CES2) was added to each solution and the solutions incubated at 37° C. for the time periods set forth in Table 5. A sample of each solution was removed at the time period and the reaction stopped with methanol and the samples evaluated and quantified for any remaining IDMF and DMF as well as, in the case of IDMF, IMMF formation and, in the case of DRF, N-(2-hydroxyetheyl) succinimide (HES). Although tolerable for normal patients, HES is undesirable and detrimental for those individuals have kidney and/or liver issues
[0106] The results are presented in Table 6. As seen in Table 6, DRF had a very slow, but steady, conversion to MMF with a reciprocal conversion and buildup of HES. As with the prior showing, IDMF had an initial, quick generation of MMF, evident of the conversion of IDMF to IMMF and MMF, followed by a longer term, slower generation of MMF indicative of the hydrolysis of IMMF to MMF and isosorbide.
TABLE-US-00006 TABLE 6 Time (minutes) DRF (mM) HES (mM) IDMF (mM) IMMF (mM) 0 1 ND 1.086 ND 15 .652 0.075 0.113 1.181 30 0.482 0.103 ND 1.585 60 0.273 0.127 ND 1.713 120 0.08 0.558 ND 1.721
[0107] Without further elaboration, it is believed that one skilled in the art, using the preceding description, can utilize the present invention to its fullest extent. Furthermore, while the present invention has been described with respect to aforementioned specific embodiments and examples, it should be appreciated that other embodiments, changes and modifications utilizing the concept of the present invention are possible, and within the skill of one in the art, without departing from the spirit and scope of the invention. The preceding preferred specific embodiments are, therefore, to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.