SYSTEM AND METHOD FOR IN-SITU AMELIORATIONREMEDIATION OF SALINE-ALKALI SOIL USING MICROALGAE
20230255154 · 2023-08-17
Assignee
Inventors
Cpc classification
International classification
Abstract
A system and method for in-situ amelioration of saline-alkali soil using microalgae. Sewage and microalgal seeds are pumped into an microalgae growth pond for growing microalgae, and online equipment is used for monitoring the level of nutrients in the pond. When the microalgae reach the stationary phase of their growth, the screen mesh is lifted to separate microalgae from liquid, so that they are conveniently harvested by scraping. The harvested microalgae are returned to the soil as a soil ameliorant.
Claims
1. A system for in-situ amelioration of saline-alkali soil using microalgae, comprising a top cover, a microalgae growth pond, a screen mesh, a soil layer, and an impermeable layer, wherein: the top cover is provided to fit over the top of the microalgae growth pond; the screen mesh is laid at least at the bottom of the microalgae growth pond; the soil layer is at least under the screen mesh to form at least the bottom of the microalgae growth pond; and the impermeable layer is laid under the soil layer.
2. The system according to claim 1, wherein the screen mesh is made of polyamides, a pore diameter of the screen mesh is less than 10 μm.
3. The system according to claim 1, wherein the top cover is made of polymethyl methacrylate or plastic film.
4. The system according to claim 1, wherein a depth of the microalgae growth pond is 15-20 cm.
5. The system according to claim 1, wherein a thickness of the soil layer is 20-40 cm.
6. The system according to claim 5, wherein the thickness of the soil layer is 30 cm.
7. A method for in-situ amelioration of the saline-alkali soil using microalgae according to claim 1, comprising the following steps: inoculating a microalgae cell solution into a microalgae growth pond, conveying sewage to the microalgae growth pond, and culturing the microalgae; a large amount of extracellular secretions secreted by the microalgae into the surrounding area during the growth process, which infiltrate into a soil layer below the microalgae growth pond, improving the physicochemical properties of the saline-alkali soil; lifting a screen mesh at the end of the microalgae culture cycle, and allowing the screen mesh to stand sufficiently to drain off the water so that the microalgae are trapped on the screen mesh; scraping the microalgae on the screen mesh for harvesting for subsequent utilization; after harvesting, the screen mesh is returned to the microalgae growth pond and the microalgae remaining on the screen mesh are used as seed cells for the next culture cycle.
8. The method according to claim 7, wherein an initial biomass concentration in the microalgae growth pond is 0.2-0.5 g/L.
9. The method according to claim 7, wherein a culture period is 6-10 days.
10. The method according to claim 7, wherein a concentration of each nutrient in the microalgae growth pound is monitored online during the culturing of the microalgae.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
[0022] The accompanying drawings, which are incorporated in and constitute a part of the present invention, are included to provide a further understanding of the present invention, and the description of the exemplary embodiments and illustrations of the present invention are intended to explain the present invention and do not constitute improper limits to the present invention.
[0023]
[0024] 1: microalgae growth pond; 2: screen mesh; 3: top cover; 4: online monitoring system; 5: impermeable layer.
DETAILED DESCRIPTION
[0025] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the present invention. Unless otherwise specified, all of the technical and scientific terms used in the present invention have the same meaning as commonly understood by ordinary technicians in the art to which the present invention belongs.
[0026] As described herein, a system is provided for in-situ amelioration of saline-alkali soil using microalgae. The system comprises a top cover, a microalgae growth pond, a screen mesh, and a soil layer, wherein,
[0027] the microalgae growth pond is provided with a top opening, and the top cover can be closed over the top opening;
[0028] the screen mesh is laid within the microalgae growth pond;
[0029] the soil layer is located at least below the microalgae growth pond to form at least the bottom of the microalgae growth pond;
[0030] an impermeable layer is laid underneath the soil layer.
[0031] The impermeable layer is used to intercept nutrients that have leaked out of the soil layer and to avoid nutrient loss.
[0032] In some embodiments, the material of the screen mesh is polyamide (nylon), and a pore diameter of the screen mesh is less than 10 μm. The screen mesh made of polyamide with the pore diameter less than 10 μm is laid within the microalgae growth pond to effectively protect most of the microalgae from infiltrating the soil and prevent the soil particles from entering the microalgae growth pond and creating difficult separation.
[0033] In some embodiments, the top cover is made of polymethyl methacrylate or plastic film.
[0034] In seasons with little rainfall, the water in the microalgae growth pond tends to evaporate under sunlight, so that it tends to dry out over time, which in turn affects the microalgae culture.
[0035] The top cover is made of a transparent material, which allows sunlight to pass through and ensure the normal growth of microalgae. When the top opening of the microalgae growth pond is covered by the top cover, the evaporated water in the microalgae growth pond condenses on the top cover and flow back into the microalgae growth pond, which effectively prevents the evaporation of water from the microalgae growth pond and ensures the normal growth of microalgae over a longer period of time.
[0036] In some embodiments, a depth of the microalgae growth pond is 15-20 cm.
[0037] In some embodiments, a thickness of the soil layer is 20-40 cm, preferably 30 cm.
[0038] A method is provided for in-situ amelioration of the saline-alkali soil using microalgae. The method comprises the following steps:
[0039] inoculating a microalgae cell solution into a microalgae growth pond, conveying sewage to the microalgae growth pond, and culturing the microalgae;
[0040] a large amount of extracellular secretions secreted by the microalgae into the surrounding area during the growth process, which infiltrate into a soil layer below the microalgae growth pond, improving the physicochemical properties of the saline-alkali soil and reshaping the structure of the soil microbial community;
[0041] lifting a screen mesh at the end of the microalgae culture cycle, and allowing the screen mesh to stand sufficiently to drain off the water so that the microalgae are trapped on the screen mesh;
[0042] scraping the microalgae on the screen mesh for harvesting for subsequent utilization;
[0043] after harvesting, the screen mesh is returned to the microalgae growth pond and the microalgae remaining on the screen mesh are used as seed cells for the next culture cycle.
[0044] In some embodiments, an initial biomass concentration in the microalgae growth pond is 0.2-0.5 g/L.
[0045] Further, a culture period is 6-10 days.
[0046] In some embodiments, a concentration of each nutrient in the microalgae growth pound is monitored online during the culturing of the microalgae.
[0047] The present invention will be further described below in conjunction with the examples.
Example 1
[0048] Amelioration of saline-alkali soils in a coastal area in Shandong Province.
[0049] The specific process is shown in
[0050] Step (1): cultivation of microalgae seeds solution:
[0051] The Spirulina Subsalsa was selected as the algae species, the standard Spirulina medium (SP medium) was selected for growing the algal seeds of Spirulina subsalsa. The components of SP medium were shown in Table 1.
TABLE-US-00001 TABLE 1 Component Dosage NaHCO.sub.3 13.61 g/L Na.sub.2CO.sub.3 4.03 g/L K.sub.2HPO.sub.4 0.50 g/L NaNO.sub.3 2.50 g/L K.sub.2SO.sub.4 1.00 g/L NaCl 1.00 g/L MgSO.sub.4•7H.sub.2O 0.20 g/L CaCl.sub.2•2H.sub.2O 0.04 g/L FeSO.sub.4•7H.sub.2O 0.01 g/L A5 (Trace metal solution*) 1 mL/L *The components of A5 are shown in Table 2.
TABLE-US-00002 TABLE 2 Components Dosage* H.sub.3BO.sub.3 2.86 g/L dH.sub.2O MnCl.sub.2•4H.sub.2O 1.86 g/L dH.sub.2O ZnSO.sub.4•4H.sub.2O 0.22 g/L dH.sub.2O Na.sub.2MoO.sub.4•2H.sub.2O 0.39 g/L dH.sub.2O CuSO.sub.4•6H.sub.2O 0.08 g/L dH.sub.2O Co(NO.sub.3).sub.2•6H.sub.2O 0.05 g/L dH.sub.2O *The abbreviation “dH.sub.2O” stands for distilled water.
[0052] The culture was incubated in a volume of 100 L at 25° C. under 24 hours of continuous light (intensity 2500 Lux) for 10 days. After the culture was finished, standing for a period of time, and then harvesting the Spirulina Subsalsa.
[0053] Step (2): construction of the microalgae growth pond:
[0054] The microalgae growth pond was built from the saline-alkali soils, with a size of 1 m in length, 0.5 m in width, and 0.2 m in depth, an effective volume of the microalgae growth pond is 0.1 m.sup.3, wherein a screen mesh, with a pore diameter of 6.5 μm, was laid within the microalgae growth pond. A soil layer of 0.3 m depth or thickness below the bottom or outside the side walls of the microalgae growth pond is the soil layer to be ameliorated and an impermeable layer is laid outside the soil layer to be ameliorated. About 80 L of sewage water was pumped into the microalgae growth pond covered with a transparent top cover made of polymethyl methacrylate.
[0055] Step (3): Culture of microalgae:
[0056] The Spirulina subsalsa harvested in Step (1) was inoculated into the microalgae growth pond in Step (2) with an initial biomass concentration of 0.4 g/L and incubated for 9 days. The sewage had an initial nitrogen concentration of 250 mg/L, an initial phosphorus concentration of 7 mg/L, an initial salinity concentration of 0.1%, an initial pH value of 7.5 and an initial DO value of 6 mg/L.
[0057] Step (4): harvesting of microalgal biomass
[0058] After 9 days of cultivation, lifting the nylon screen mesh and allowing the screen mesh to stand sufficiently to drain off the water, scraping the Spirulina subsalsa trapped in the screen mesh into a sterile container and storing in a −20° C. refrigerator. The Spirulina subsalsa remaining in the screen mesh together with the screen mesh were returned to the microalgae growth pond in step (2) as the initial algae species for the second culture cycle, and the newly cultured Spirulina subsalsa species from step (1) was added and the initial biomass concentration in the microalgae growth pond was adjusted to 0.4 g/L to start the second culture cycle.
[0059] Step (5): resource utilisation of microalgae:
[0060] The spirulina subsalsa harvested in step (4) was freeze-dried to constant weight in a freeze-dryer and fully ground into a powder, which was measured using spectrophotometry at 10% Spirulina cyanobacteria content.
[0061] The spirulina subsalsa harvested in step (4) was freeze-dried to a constant mass in a freeze dryer and fully ground to a powder. The content of spirulina phycocyanin was measured to be 10% using spectrophotometric methods, which can be used for the production of spirulina phycocyanin.
[0062] After 3-5 culture cycles, the soil conductivity was decreased by 22%, the soil salinity was reduced by 20%, 80 g of the spirulina powder was harvested, and 5 g of the spirulina phycocyanin was extracted
[0063] The foregoing descriptions are merely preferred embodiments of the present invention, and are not intended to limit the present invention. For a person skilled in the art, the present invention may have various modifications and changes. Any modification, equivalent replacement, or improvement made without departing from the spirit and principle of the present invention shall fall within the protection scope of the present invention.