APPLICATION OF ATRACTYLODES LANCEOLATA ESSENTIAL OIL

Abstract

The present invention provides an application of Atractylodes lanceolata essential oil, which is used for the prevention and treatment of Pseudopestalotiopsis camelliae sinensis. The Atractylodes lanceolata essential oil of the present invention has a good inhibitory effect on Pseudopestalotiopsis camelliae sinensis, and can be developed and used as a new type of plant-derived fungicide.

Claims

1. An application of Atractylodes lanceolata essential oil, wherein the Atractylodes lanceolata essential oil is used for the prevention and treatment of Pseudopestalotiopsis camelliae-sinensis.

2. The application of Atractylodes lanceolata essential oil according to claim 1, wherein EC.sub.50 of laboratory virulence of the Atractylodes lanceolata essential oil to Pseudopestalotiopsis camelliae-sinensis measured is 0.269 mg/mL.

Description

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0010] In the application of the Atractylodes lanceolata essential oil in this embodiment, the Atractylodes lanceolata essential oil is used for the prevention and treatment of Pseudopestalotiopsis camelliae-sinensis; EC.sub.50 of the laboratory virulence of the Atractylodes lanceolata essential oil on Pseudopestalotiopsis camelliae-sinensis is 0.269 mg/mL.

Example 1

[0011] The Atractylodes lanceolata essential oil was obtained by steam distillation. The specific method was as follows:

[0012] 200 g of Atractylodes powder was filtered through a 26-mesh sieve and placed in a 5000-mL round-bottomed flask. 3000 mL of water was added and the powder was allowed to soak overnight, and then was heated on a thermostatic electric heating mantle to boil and extract for 6 hours, then heating was stopped. The condensed water flowed back to the extractor for cooling until no liquid flowed down from the condenser to collect the Atractylodes lanceolata essential oil.

[0013] (1) Laboratory virulence determination of the Atractylodes lanceolata essential oil on Pseudopestalotiopsis camelliae-sinensis

[0014] A small amount of dimethyl sulfoxide was added to Atractylodes lanceolata essential oil, and then diluted with sterilized distilled water to obtain a medicinal solution. The fungicides were added to the PDA medium to prepare the Atractylodes lanceolata essential oil 1 at concentrations of 0.10, 0.50, 0.75, 1.00 mg/mL drug-containing medium, and PDA medium without medicament as a control. Each treatment was repeated 3 times. After 5 days of cultivation of the pathogen, a punch with a diameter of 5 mm was used to punch out the fungus block, followed by inoculation to the center of each fungicide-containing medium and the control PDA medium, and then the fungal colony-containing side was fully contact the medium and incubated for 7 days in a constant temperature incubator at 25±2° C. The fungal colony diameter was then measured by the cross method and the average value taken. The SPSS software was used to calculate the inhibition rate, toxicity regression equation and the EC.sub.50 value. The determination results are shown in Table 1, indicating that the Atractylodes lanceolata essential oil has a significant inhibition rate on Pseudopestalotiopsis camelliae-sinensis. When the concentration was 1.00 mg/mL, the inhibition rate was 100%.

[0015] Inhibition Rate Calculation Formula:

Inhibition rate=(Control colony diameter−Treated colony diameter)/Control colony diameter×100%

TABLE-US-00001 TABLE 1 Laboratory virulence of the atractylodes lanceolata essential oil on Pseudopestalotiopsis camelliae-sinensis Concen- Toxicity tration Inhibition regression Correlation EC.sub.50 Fungicide (mg/mL) rate (%) equation coefficient (mg/mL) Atractylodes 0.10 22.67 y = 0.986 0.269 lanceolata 0.50 60.00 2.068 ×+ essential oil 0.75 76.00 6.180 1.00 100.00

[0016] (2) Field antifungal effect of Atractylodes lanceolata oil on Pseudopestalotiopsis camelliae-sinensis

[0017] Sixty healthy tea seedlings were selected from 1-2 years old, and the pathogen of rotifer was formulated into a conidia suspension of 1×10.sup.6 spores/mL to spray the healthy tea seedlings. Then the tea seedlings treated with pathogen were divided into two groups, namely the treatment group and the clear water control group, each group containing 30 tea seedlings. At the early stage of the occurrence of tea gray blight disease, the treatment group was sprayed with 1.00 mg/mL Atractylodes essential oil aqueous solution, and the clear water control group was treated with clear water. In the peak period of the disease, the disease index and the control effect were calculated according to the disease status of tea leaves. The classification standards were as follows:

TABLE-US-00002 TABLE 2 Classification standards of tea gray blight disease Degree Symptom 0 degree Anosis 1 degree 1 to 2 lesions on the leaf 2 degree 3 to 4 lesions on the leaves or less than two-third of the leaf area 3 degree 5 to 7 lesions on the leaves or a half of the leaf area 4 degree More than 8 lesions on the leaves or more than two-third of the leaf area, or there are lesions on petioles or tender leaves

[0018] The formula for calculating the disease index was:

Disease index=100×Σ(Number of diseased leaves at each degree×Representative value of each degree)/(Total number of leaves in investigation×Representative value of highest degree1)

Control effect (%)=100×(Disease index of control area−Disease index of the treatment area)/Disease index of clear water control group.

TABLE-US-00003 TABLE 3 Field control effect of Atractylodes lanceolata essential oil on Pseudopestalotiopsis camelliae-sinensis Treatment Disease index Control effect (%) Atractylodes lanceolata 1.83 91.93 essential oil Clear water control group 22.67 — Note: “—” means no investigation has been done.

[0019] It can be seen from Table 3 that the use of Atractylodes lanceolata essential oil to prevent and treat Pseudopestalotiopsis camelliae-sinensis can achieve 91.93% of the control effect, which has a good control effect.

[0020] The above are merely preferred embodiments of the present invention, but do not impose any limitation on the present invention. Any simple modifications, alterations and equivalent changes made to the above embodiments based on the technical essence of the invention still fall within the protection scope of the technical solution of the present invention.