Thienodiazepine derivatives and application thereof

Abstract

The present invention relates to a class of thienodiazepine derivatives and an application thereof in the preparation of a drug for the treatment of diseases associated with bromodomain and extra-terminal (BET) Bromodomain inhibitors. Specifically, the present invention relates to compounds represented by formulas (I) and (II), as well as pharmaceutically acceptable salts thereof. ##STR00001##

Claims

1. A compound represented by formula (I) or (II), ##STR00229## or a geometric isomer, a stereoisomer, a tautomer, or a pharmaceutically acceptable salt thereof, wherein, T is C or N; R.sub.1 is C.sub.1-3 alkyl or C.sub.1-3 alkoxyl, wherein the C.sub.1-3 alkyl or C.sub.1-3 alkoxyl is optionally substituted by 1, 2 or 3 independently selected R substituents; each of R.sub.2, R.sub.3, and R.sub.4 is independently H, F, Cl, Br, I, OH, NH.sub.2, CN, C.sub.1-6 alkyl, or C.sub.1-6 heteroalkyl, wherein the C.sub.1-6 alkyl or C.sub.1-6 heteroalkyl is optionally substituted by 1, 2 or 3 independently selected R substituents, wherein the heteroatoms are 1, 2, 3, or 4 of N, —O—, —S—, —NH—, —C(═O)NH—, —C(═O)—, —C(═O)O—, —S(═O).sub.2—, —S(═O)—, or —C(═O)S—; R.sub.5 is H, or C.sub.1-3 alkyl that is optionally substituted by 1, 2 or 3 independently selected R substituents; each R.sub.6 is independently H, F, Cl, Br, I, OH, NH.sub.2, CN, C.sub.1-6 alkyl, or C.sub.1-6 heteroalkyl, wherein the C.sub.1-6 alkyl or C.sub.1-6 heteroalkyl is optionally substituted by 1, 2 or 3 independently selected R substituents, wherein the heteroatoms are 1, 2, 3, or 4 of N, —O—, —S—, —NH—, —C(═O)NH—, —C(═O)—, —C(═O)O—, —S(═O)2-, —S(═O)—, or —C(═O)S—; or two R.sub.6 groups attached to the same carbon atom form C(═O) together with the carbon atom attached thereto; either ring A is a C.sub.3-7 cycloalkyl, a 5 or 7-12 membered heterocycloalkyl, or a 5-6 membered heteroaryl, wherein the heteroatoms are 1, 2, 3, or 4 of N, —O—, —S—, —NH—, —C(═O)NH—, —C(═O)—, —C(═O)O—, —S(═O)2-, —S(═O)—, or —C(═O)S—, or ring A is a 6 membered heterocycloalkyl, wherein the heteroatoms are 1, 2, 3, or 4 of N, —O—, —S—, —C(═O)—, —C(═O)O—, —S(═O)2-, —S(═O)—, or —C(═O)S—, provided that the structural unit ##STR00230## is not selected from a group consisting of ##STR00231## ring B is 4-7 membered heterocycloalkyl, wherein the heteroatoms are 1, 2, 3, or 4 of N, —O—, —S—, —NH—, —C(═O)NH—, —C(═O)—, —C(═O)O—, —S(═O)2-, —S(═O)—, or —C(═O)S—; n is 0, 1, 2, 3, 4, 5, or 6; each R is independently F, Cl, Br, I, OH, NH.sub.2, CN, C.sub.1-6 alkyl, or C.sub.1-6 heteroalkyl, wherein the C.sub.1-6 alkyl or C.sub.1-6 heteroalkyl is optionally substituted by 1, 2 or 3 independently selected R′ substituents, wherein the heteroatoms are 1, 2, 3, or 4 of N, —O—, —S—, —NH—, —C(═O)NH—, —C(═O)—, —C(═O)O—, —S(═O)2-, —S(═O)—, or —C(═O)S—; each R′ is independently F, Cl, Br, I, OH, NH.sub.2, CN, or Me; and the carbon atom marked with “*” is a chiral carbon atom, which is present in the form of a single (R) or (S) enantiomer, or in the form of being enriched in one of two enantiomers.

2. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, wherein each R is independently F, Cl, Br, I, OH, NH.sub.2, CN, C.sub.1-3 alkyl, or C.sub.1-3 alkoxyl, wherein each C.sub.1-3 alkyl and C.sub.1-3 alkoxyl is optionally substituted by 1, 2 or 3 independently selected R′ substituents.

3. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 2, wherein each R is independently F, Cl, Br, I, OH, NH.sub.2, CN, Me, CF.sub.3, Et, or ##STR00232##

4. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, wherein R.sub.1 is selected from a group consisting of Me, Et and ##STR00233## all of which are optionally substituted by 1, 2 or 3 independently selected R substituents.

5. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, wherein R.sub.2, R.sub.3, and R.sub.4 are independently H, F, Cl, Br, I, OH, NH.sub.2, CN, C.sub.1-3 alkyl, or C.sub.1-3 alkoxy, wherein each C.sub.1-3 alkyl and C.sub.1-3 alkoxy is optionally substituted by 1, 2 or 3 independently selected R substituents.

6. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt according to claim 5, wherein R.sub.2, R.sub.3, and R.sub.4 are independently H, F, Cl, Br, I, OH, NH.sub.2, CN, Me, or ##STR00234##

7. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, wherein R.sub.5 is H or Me.

8. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, wherein each R.sub.6 is independently H, F, Cl, Br, I, OH, NH.sub.2, CN, C.sub.1-3 alkyl, or C.sub.1-3 heteroalkyl, wherein each C.sub.1-3 alkyl and C.sub.1-3 heteroalkyl is optionally substituted by 1, 2 or 3 independently selected R substituents.

9. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 8, wherein each R.sub.6 is independently H, F, Cl, Br, I, OH, NH.sub.2, CN, Me, Et, ##STR00235## wherein the Me, Et, ##STR00236## is optionally substituted by 1, 2 or 3 independently selected R substituents.

10. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 9, wherein each R.sub.6 is independently H, F, Cl, Br, I, OH, NH.sub.2, CN, Me, Et, ##STR00237##

11. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, wherein ring A is C.sub.4-6 cycloalkyl, pyrrolidin-2-onyl, pyrimidin-4(3H)-onyl, 5-azaspiro[2.4]heptan-4-onyl, 4-azaspiro[2.4]heptan-5-onyl, tetrahydrothiophene-1,1-dioxide group, tetrahydrothiophene-1-oxide group, tetrahydrofuranyl, pyrrolidinyl, dihydrothiophene-2(3H)-onyl, 2-oxaspiro[3.4]octyl, dihydrofuran-2(3H)-onyl, 1,4,7,10-tetraoxacyclododecyl, 1,2,5-oxadiazolyl, 7-oxabicyclo-[2.2.1]heptane, pyrrolidin-2,5-dione, or 5,5-dimethyl-dihydrofuran-2(3H)-onyl.

12. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, wherein the structural unit ##STR00238## is ##STR00239## ##STR00240##

13. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 12, wherein the structural unit ##STR00241## is ##STR00242## ##STR00243##

14. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 13, wherein the structural unit ##STR00244## is ##STR00245## ##STR00246##

15. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, wherein the structural unit ##STR00247## is ##STR00248##

16. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, which is ##STR00249## ##STR00250## ##STR00251## ##STR00252## wherein, T.sub.1 is —S(═O)—, —S(═O).sub.2—, —N(R.sub.6)—, —O—, —C(R.sub.6)(R.sub.6)—, or ##STR00253## T.sub.2 is independently —NH—, —O—, or —S—; R.sub.1-R.sub.6 are as defined in claim 1; and the carbon atom marked with “*” is a chiral carbon atom, which is present in the form of a single (R) or (S) enantiomer, or in the form of being enriched in one of two enantiomers.

17. The compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1, wherein the compound is ##STR00254## ##STR00255## ##STR00256## ##STR00257## ##STR00258## ##STR00259## ##STR00260## ##STR00261## ##STR00262## ##STR00263##

18. A pharmaceutical composition, comprising a therapeutically effective amount of the compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1 as an active ingredient and a pharmaceutically acceptable carrier.

19. A method of treating a disease, wherein the disease comprises breast cancer, triple negative breast cancer, colon cancer, rectal cancer, colorectal cancer or prostatic cancer, the method comprising administering a therapeutically effective amount of the compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1 to a subject in need thereof.

20. The method of claim 19, wherein the disease is a bromodomain extra-terminal (BET) protein-related disease.

21. A method of inhibiting BET bromodomain protein activity, comprising administering an effective amount of the compound, geometric isomer, stereoisomer, tautomer, or pharmaceutically acceptable salt of claim 1 to a subject in need thereof.

Description

A DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

(1) The present invention will be specifically described below by way of examples, but it does not imply any disadvantageous limitation to the present invention. The present invention has been described in detail herein, and its specific embodiments are also disclosed. It will be obvious to those skilled in the art that various changes and improvements can be made to the specific embodiments of the present invention without departing from the spirit and scope of the present invention.

(2) ##STR00040## ##STR00041## ##STR00042##

Example 1

(3) ##STR00043##

(4) Synthesis of Compound 1-2

(5) ##STR00044##

(6) Compound 1-1 (25.00 g, 139.20 mmol, 1.00 eq), 2-butanone (11.04 g, 153.12 mmol, 13.63 mL, 1.10 eq) and morpholine (12.13 g, 139.20 mmol, 12.25 mL, 1.00 eq) were dissolved in ethanol (200.00 mL), and a sublimed sulfur (4.46 g, 139.20 mmol, 1.00 eq) was added. The suspension was warmed up to 70° C. and stirred under the protection of nitrogen gas for 12 hours. The reaction mixture was concentrated under reduced pressure to give a yellow oil. Water (500 mL) was added to the oily substance, and the resulting mixture was extracted with ethyl acetate (200 mL×4). The combined organic phases were collected, washed with a saturated saline solution (200 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The obtained crude product was purified by a silica gel column (petroleum ether/ethyl acetate=10/1) to give the compound 1-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.47 (d, J=8.0 Hz, 2H), 7.38 (d, J=8.0 Hz, 2H), 6.43 (br s, 2H), 2.13 (s, 3H), 1.56 (s, 3H).

(7) Synthesis of Compound 1-3

(8) ##STR00045##

(9) Compound 1-2 (10.00 g, 37.63 mmol, 1.00 eq) was dissolved in chloroform (100.00 mL) and 2-chloroacetyl chloride (6.37 g, 56.45 mmol, 4.49 mL, 1.50 eq) was added dropwisely. After the completion of the dropwise addition, the reaction mixture was stirred at 70° C. for 1 hour. The reaction mixture was washed with a saturated sodium bicarbonate solution (100 mL) and a saturated saline solution (50 mL), then dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure. The obtained compound as a crude product was recrystallized with methanol (40 mL) to give the compound 1-3. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 11.81 (br s, 1H), 7.58 (dd, J=2.0, 6.4 Hz, 2H), 7.45 (dd, J=2.2, 8.6 Hz, 2H), 4.25 (s, 2H), 2.29 (s, 3H), 1.72 (s, 3H).

(10) Synthesis of Compound 1-4

(11) ##STR00046##

(12) Compound 1-3 (11.00 g, 32.14 mmol, 1.00 eq) and sodium iodide (9.63 g, 64.28 mmol, 2.00 eq) were added to tetrahydrofuran (50.00 mL). The resulting mixture was stirred at 60° C. for 2 hours. The reaction mixture was directly concentrated under reduced pressure to give compound 1-4, which was not purified and directly used in the next step of the reaction. LCMS (ESI) m/z: 433.9 (M+1).

(13) Synthesis of Compound 1-5

(14) ##STR00047##

(15) Compound 1-4 (14.00 g, 32.28 mmol, 1.00 eq) was dissolved in tetrahydrofuran (100.00 mL). The resulting mixture was cooled to −60° C. and ammonia gas was charged for 30 minutes. The resulting reaction mixture was slowly warmed up to 20° C. and stirred for 3 hours. The reaction mixture was directly concentrated under reduced pressure. The obtained solid was dissolved in ethyl acetate (150 mL) and washed with water (50 mL×3) and a saturated saline solution (50 mL), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure. The obtained compound 1-5 was directly used in the next step of the reaction. LCMS (ESI) m/z: 322.9 (M+1), 344.9 (M+Na).

(16) Synthesis of Compound 1-6

(17) ##STR00048##

(18) Compound 1-5 (10.00 g, 30.98 mmol, 1.00 eq) was dissolved in isopropanol (150.00 mL) and glacial acetic acid (50.00 mL). The resulting mixture was stirred at 90° C. for 3 hours. The solvent was removed under reduced pressure from the reaction mixture. The residual mixture was dissolved in chloroform (20 mL), washed with a saturated sodium bicarbonate solution (20 mL) and a saturated saline solution (20 mL), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure. The compound as a crude product was recrystallized with ethyl acetate (50 mL) to give compound 1-6. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.98 (br s, 1H), 7.46 (d, 8.4 Hz, 2H), 7.35 (d, 8.4 Hz, 2H), 4.80 (d, J=8.8 Hz, 1H), 3.93 (d, J=8.6 Hz, 1H), 2.28 (s, 3H), 1.59 (s, 3H).

(19) Synthesis of Compound 1-7

(20) ##STR00049##

(21) Phosphorus pentasulfide (17.07 g, 76.79 mmol, 8.17 mL, 3.60 eq) was added to a constantly stirred turbid liquor of sodium carbonate (4.07 g, 38.39 mmol, 1.80 eq) in 1,2-dichloroethane (200.00 mL). The resulting mixture was stirred at 20° C. for 1 hour. Then compound 1-6 (6.50 g, 21.33 mmol, 1.00 eq) was added. The obtained turbid liquor was reacted at 65° C. for 5 hours. The reaction mixture was cooled to 20° C. and filtered. The filter cake was dissolved in ethyl acetate (2 L) and washed with a saturated saline solution (500 mL), dried over sodium sulfate, filtered, and then concentrated under reduced pressure. The compound as a crude product was purified with a silica gel column (petroleum ether/ethyl acetate=5/1) to give compound 1-7.

(22) Synthesis of Compound 1-8

(23) ##STR00050##

(24) At 0° C., to a turbid liquor of compound 1-7 (3.50 g, 10.91 mmol, 1.00 eq) in methanol (5.00 mL) was added hydrazine hydrate (1.67 g, 32.72 mmol, 1.62 mL, 98% purity, 3.00 eq). The mixture was reacted under being stirred at 0° C. for 1 hour. The reaction mixture was filtered, and the filter cake was oven-dried. Compound 1-8 was obtained and directly used in the next step of the reaction. LCMS (ESI) m/z: 318.9 (M+1).

(25) Synthesis of Compound 1-9

(26) ##STR00051##

(27) To a mixed liquor of compound 1-8 (2.50 g, 7.84 mmol, 1.00 eq) in toluene (100.00 mL) was added triethyl orthoacetate (3.82 g, 23.52 mmol, 4.29 mL, 3.00 eq). The resulting mixture was reacted under being stirred at 80° C. for 1 hour. The reaction mixture was directly concentrated under reduced pressure. The compound as a crude product was recrystallized with ethyl acetate (10 mL) to give compound 1-9. LCMS (ESI) m/z: 344.9 (M+1).

(28) Synthesis of Compound 1-10

(29) ##STR00052##

(30) At −70° C., to a solution of compound 1-9 (1.50 g, 4.38 mmol, 1.00 eq) in tetrahydrofuran (180 mL), was added dropwisely LiHMDS (1 M, 8.76 mL, 2.00 eq). The mixture was reacted under being stirred at the same temperature for 1 hour, and then a solution of tert-butyl 2-bromoacetate (1.28 g, 6.57 mmol, 970.82 μL, 1.50 eq) dissolved in tetrahydrofuran (20 mL) was added dropwisely. After the completion of the dropwise addition, the reaction mixture was slowly warmed up to 20° C. and stirred for 5 hours. The reaction mixture was quenched with a saturated NH4Cl solution (50 mL), extracted with ethyl acetate (100 mL) and washed with a saturated saline solution (50 mL), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure. The compound as a crude product was purified with a flash chromatography column, and the obtained compound was separated with SFC to give compound 1-10 (basicity-EtOH, chromatography column: AS (250 mm×30 mm, 5 μm), mobile phase B: 30%, flow rate (mL/min): 55) ([α].sup.25.sub.D+54 (C 0.6, CHCl.sub.3)). LCMS (ESI) m/z: 457.0 (M+1).

(31) Synthesis of Compound 1-11

(32) ##STR00053##

(33) Compound 1-10 (150.00 mg, 328.23 μmol, 1.00 eq) was dissolved in methylene chloride (5.00 mL) and trifluoroacetic acid (1.00 mL). The mixture was reacted under being stirred at 20° C. for 4 hours. The reaction mixture was directly concentrated under reduced pressure. Compound 1-11 was obtained and directly used in the next step of the reaction. LCMS (ESI) m/z: 401.0 (M+1).

(34) Synthesis of Compound 1

(35) ##STR00054##

(36) At 30° C. and under the protection of nitrogen gas, N,N-diisopropylethylamine (48.36 mg, 374.19 μmol) was slowly added dropwisely to a solution of compound 1-11 (50.00 mg, 124.73 μmol), 6-amino-isoindolin-1-one (27.72 mg, 187.10 μmol) and HATU (71.14 mg, 187.10 μmol) in anhydrous methylene chloride (15.00 mL). After the addition, the mixture was reacted at 30° C. for 12 hours. The reaction mixture was washed with water (20 mL×2). The aqueous phase was extracted with methylene chloride (20 mL). The combined organic phases were dried over anhydrous sodium sulfate, filtered, then concentrated under reduced pressure, and purified with a preparative chromatography to give compound 1. .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 9.46 (br s, 1H), 8.03 (br s, 1H), 7.85 (d, J=7.6 Hz, 1H), 7.41 (d, J=8.4 Hz, 2H), 7.31-7.33 (m, 3H), 6.57-6.61 (m, 1H), 4.69-4.73 (m, 1H), 4.34 (s, 2H), 3.83-3.88 (m, 1H), 3.56-3.61 (m, 1H), 2.69 (s, 3H), 2.41 (s, 3H), 1.68 (s, 3H). LCMS (ESI) m/z: 531.1 (M+1).

(37) ##STR00055##

Example 2

(38) ##STR00056##

(39) Example 2 was synthesized with reference to Example 1.

(40) .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 9.62 (br s, 1H), 8.00 (s, 1H), 7.76 (d, J=8.4 Hz 1H), 7.34-7.45 (m, 5H), 6.14-6.15 (m, 1H), 4.65-4.68 (m, 1H), 4.38 (s, 2H), 3.85-3.91 (m, 1H), 3.50-3.54 (m, 1H), 2.71 (s, 3H), 2.44 (s, 3H), 1.72 (s, 3H). LCMS (ESI) m/z: 531.1 (M+1).

(41) Schemes 3 and 4

(42) ##STR00057##

Examples 3 and 4

(43) ##STR00058##

(44) Synthesis of Compound 3-2

(45) ##STR00059##

(46) At 0° C., magnesium methyl bromide (3 M, 6.30 mL) was added to anhydrous diethyl ether (10 mL). The atmosphere was replaced with nitrogen gas three times. A solution of compound 3-1 (2.00 g, 15.13 mmol) in anhydrous diethyl ether (40 mL) was slowly added dropwisely. The mixture was stirred at 25° C. in a nitrogen atmosphere for 3 hours. The reaction mixture was poured into ice water (50 g) under being stirred. A saturated NH.sub.4Cl solution (50 mL) was added and the mixture was stirred for 5 minutes. The mixture was separated into two phases. The aqueous phase was extracted with ethyl acetate (50 mL). The organic phases were combined and washed with a saturated sodium bicarbonate solution (50 mL), water (50 mL) and a saturated saline solution (80 mL) each once, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a flash chromatography column to give compound 3-2. .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 7.14-7.23 (m, 4H), 2.97-3.08 (m, 4H), 1.52 (s, 3H).

(47) Synthesis of Compound 3-3

(48) ##STR00060##

(49) At 0° C. and under being stirred, a solution of compound 3-2 (200.00 mg, 1.35 mmol) in methylene chloride (2 mL) was slowly added to a solution of concentrated nitric acid (4.20 g, 66.66 mmol, 3.00 mL) and concentrated sulphuric acid (132.36 mg, 1.35 mmol) in anhydrous methylene chloride (10 mL). The mixture was stirred at 0° C. for 5 minutes. The reaction mixture was slowly poured into crushed ice (50 g) under being stirred. The mixture was stirred for 10 minutes and separated into two phases. The aqueous phase was extracted with methylene chloride (30 mL×2). The combined organic phases were washed with a saturated sodium bicarbonate solution (80 mL) and a saturated saline solution (80 mL) respectively, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a thin-layer chromatography plate to give compound 3-3. .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 8.04-8.14 (m, 2H), 7.32-7.38 (m, 1H), 3.53-3.57 (m, 2H), 3.28-3.33 (m, 2H), 1.81 (s, 3H).

(50) Synthesis of Compound 3-4

(51) ##STR00061##

(52) Compound 3-3 (220.00 mg, 1.14 mmol) and Pd/C (200.00 mg, 10% purity) were added to absolute methanol (10.00 mL). The atmosphere was replaced with hydrogen gas three times. The mixture was stirred for 16 hours at 25° C. under a hydrogen balloon atmosphere. The reaction mixture was directly filtered through a pad of celite with a Buchner funnel and concentrated under reduced pressure. The crude product was purified with a thin-layer chromatography plate to give compound 3-4.

(53) Synthesis of Compound 3-5

(54) ##STR00062##

(55) Compound 3-4 (46.00 mg, 281.83 μmol, 1.20 eq) and diisopropylethylamine (91.06 mg, 704.58 μmol, 123.05 μL, 3.00 eq) were added to methylene chloride (5.00 mL). Compound 1-11 (94.15 mg, 234.86 μmol, 1.00 eq) and HATU (89.30 mg, 234.86 μmol, 1.00 eq) were added. The atmosphere was replaced with nitrogen gas three times. The mixture was stirred at 25° C. in a nitrogen atmosphere for 2 hours. The reaction mixture was shaking washed with water (10 mL). The organic phase was washed with a saturated saline solution (20 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a thin-layer chromatography plate to give compound 3-5. LCMS (ESI) m/z: 546.2 (M+1).

(56) Synthesis of Compounds 3 and 4

(57) ##STR00063##

(58) Compound 3-5 (98 mg, 179.46 μmol) was subjected to a SFC chiral resolution (chromatography column: AD (250 mm×30 mm, 10 μm); mobile phase: [0.1% NH.sub.3H.sub.2O EtOH]; B %: 40%-40%, 60 mL/min) to give two products, each having a single configuration. Example 3 (Rt=5.311 min). .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 8.68 (s, 1H), 7.51 (s, 1H), 7.41 (d, J=8.4 Hz, 2H), 7.33 (d, J=8.8 Hz, 2H), 7.28-7.29 (m, 1H), 7.12 (d, J=8.0 Hz, 1H), 4.60-4.64 (m, 1H), 3.74-3.80 (m, 1H), 3.44-3.49 (m, 1H), 2.95-3.03 (m, 4H), 2.68 (s, 3H), 2.40 (s, 3H), 1.68 (s, 3H), 1.49 (s, 3H). LCMS (ESI) m/z: 546.2 (M+1).

(59) Example 4 (Rt=5.926 min) .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 8.72 (s, 1H), 7.51 (s, 1H), 7.41 (d, J=8.4 Hz, 2H), 7.33 (d, J=8.8 Hz, 2H), 7.28-7.29 (m, 1H), 7.12 (d, J=8.0 Hz, 1H), 4.60-4.64 (m, 1H), 3.74-3.80 (m, 1H), 3.44-3.49 (m, 1H), 2.95-3.03 (m, 4H), 2.68 (s, 3H), 2.40 (s, 3H), 1.68 (s, 3H), 1.49 (s, 3H). LCMS (ESI) m/z: 546.1 (M+1).

(60) ##STR00064##

Example 5

(61) ##STR00065##

(62) Synthesis of Compound 5-2

(63) ##STR00066##

(64) At 0° C., thionyl chloride (25.77 g, 216.65 mmol, 15.71 mL, 1.20 eq) was added dropwisely to methanol (200.00 mL). The mixture was stirred at 0° C. for 30 minutes. A solution of compound 5-1 (30.00 g, 180.54 mmol, 25.86 mL, 1.00 eq) in methanol (100.00 mL) was added dropwisely. After the completion of the dropwise addition, the mixture was reacted at 26° C. for 4 hours. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in methylene chloride. The mixture was adjusted with a saturated sodium carbonate solution to pH=8-9, extracted, and separated into two phases. The organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 5-2, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 7.26-7.37 (m, 5H), 4.63 (s, 2H), 4.11 (s, 2H), 3.76 (s, 3H).

(65) Synthesis of Compound 5-3

(66) ##STR00067##

(67) A mixture of compound 5-2 (16.00 g, 88.79 mmol, 1.00 eq) and tert-butoxy di(dimethylamino)methane (17.02 g, 97.67 mmol, 20.26 mL, 1.10 eq) was heated to 90° C. and reacted for 16 hours. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in methylene chloride (80 mL). The mixture was washed with a saturated saline solution (25 mL), extracted, and separated into two phases. The organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 5-3, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 7.26-7.33 (m, 5H), 6.78 (s, 1H), 4.63 (s, 2H), 3.64 (s, 3H), 2.88 (m, 6H).

(68) Synthesis of Compound 5-4

(69) ##STR00068##

(70) Glacial acetic acid (40.00 mL) was added to a mixture of compound 5-3 (12.00 g, 51.00 mmol, 1.00 eq) and 4-bromo-2-aminopyridine (8.82 g, 51.00 mmol, 1.00 eq). The resulting mixture was heated to 130° C. and stirred for 16 hours. The reaction mixture was concentrated under reduced pressure. The obtained residue was purified with column chromatography (petroleum ether:ethyl acetate=4:1) to give compound 5-4. .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 8.72 (d, J=7.6 Hz, 1H), 8.19 (s, 1H), 7.89 (s, 1H), 7.35-7.47 (m, 5H), 5.19 (s, 2H).

(71) Synthesis of Compound 5-5

(72) ##STR00069##

(73) To a solution of compound 5-4 (1.00 g, 3.02 mmol, 1.00 eq) and tert-butyl carbamate (459.88 mg, 3.93 mmol, 1.30 eq) in 1,4-dioxane (20.00 mL) were successively added 4,5-bis(diphenylphosphine)-9,9-dimethylxanthene (174.73 mg, 301.97 μmol, 0.10 eq), tris(dibenzylideneacetone) dipalladium (276.52 mg, 301.97 μmol, 0.10 eq) and cesium carbonate (2.95 g, 9.06 mmol, 3.00 eq). The atmosphere was replaced with nitrogen gas three times. The mixture was heated to 100° C. under the protection of nitrogen gas and reacted for 10 hours. The reaction mixture was cooled to room temperature and filtered. The filter cake was washed with methylene chloride (10 mL×2). The resulting filtrate was concentrated under reduced pressure. The obtained residue was purified with column chromatography (petroleum ether:ethyl acetate=5:1-2:1) to give compound 5-5. LCMS (ESI) m/z: 368.2 (M+1).

(74) Synthesis of Compound 5-6

(75) ##STR00070##

(76) A mixture of compound 5-5 (190.00 mg, 517.15 μmol, 1.00 eq) and trifluoroacetic acid (5.00 mL) was heated to 90° C. and stirred for 20 hours. The reaction mixture was concentrated under reduced pressure. The resulting residue was dissolved in methylene chloride (10 mL). The mixture was again concentrated under reduced pressure to give compound 5-6, which was directly used in the next step without any further purification.

(77) Synthesis of Compound 5

(78) ##STR00071##

(79) At −10° C. and under the protection of nitrogen gas compound 1-11 (60.00 mg, 149.67 μmol, 1.00 eq) and triethylamine (30.29 mg, 299.34 μmol, 41.49 μL, 2.00 eq) were dissolved in a mixed liquor of anhydrous tetrahydrofuran (2.00 mL) and anhydrous N,N-dimethyl formamide (1.00 mL). Then pivaloyl chloride (18.05 mg, 149.67 μmol, 18.42 μL, 1.00 eq) was slowly added dropwisely to the above solution. The resulting mixture was stirred at −10° C. for 0.5 hour. Then a solution of compound 5-6 (40.00 mg, 137.37 μmol, 0.92 eq, TFA) in anhydrous N,N-dimethyl formamide (1.00 mL) was added dropwisely to the reaction mixture. After the completion of the dropwise addition, the mixture was warmed up to 27° C. and stirred for 5 hours. The reaction mixture was quenched with water (5 mL), and the mixture was separated into two phases. The aqueous phase was extracted with methylene chloride (5 mL×3). The organic phases were combined. The combined organic phases were washed with a saturated saline solution (10 mL), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The obtained crude product was purified with a preparative chromatography (basicity) to give compound 5. .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 8.76 (d, J=7.28 Hz, 1H), 8.12 (s, 1H), 7.47 (d, J=8.28 Hz, 2H), 7.34 (d, J=8.53 Hz, 3H), 6.56-6.64 (m, 2H), 5.12 (s, 1H), 4.72 (t, J=7.15 Hz, 1H), 3.93-4.00 (m, 2H), 2.69 (s, 3H), 2.40 (s, 3H), 1.68 (s, 3H). LCMS (ESI) m/z: 560.0 (M+1).

(80) ##STR00072##

Examples 6 and 7

(81) ##STR00073##

(82) Synthesis of Compound 6-2

(83) ##STR00074##

(84) Under the protection of nitrogen gas, to a solution of compound 6-1 (5.00 g, 27.02 mmol, 3.65 mL, 1.00 eq) in carbon tetrachloride (20.00 mL) were added NBS (10.00 g, 56.19 mmol, 2.08 eq) and AIBN (1.04 g, 6.33 mmol, 0.23 eq). The mixture was reacted under being stirred at 65° C. for 4 hours. The reaction mixture was directly concentrated under reduced pressure. The crude product was purified with a flash chromatography column to give compound 6-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.57 (d, J=8.0 Hz, 1H), 7.32 (d, J=8.0 Hz, 1H), 7.16 (t, J=7.8 Hz, 1H), 4.84 (s, 2H), 4.63 (s, 2H).

(85) Synthesis of Compound 6-3

(86) ##STR00075##

(87) Neutral alumina (100.00 g, 980.78 mmol, 93.41 eq) was added to a solution of compound 6-2 (3.60 g, 10.50 mmol, 1.00 eq) dissolved in n-hexane (200.00 mL), and the mixture was reacted under being stirred at 75° C. for 2 hours. The reaction mixture was directly filtered. The filter cake was washed with ethyl acetate (200 mL). The filtrate was directly concentrated under reduced pressure. The compound as a crude product was purified with a flash chromatography column to give compound 6-3. .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 7.38-7.40 (m, 1H), 7.16-7.16 (m, 2H), 5.21 (s, 2H), 5.10 (s, 2H).

(88) Synthesis of Compound 6-4

(89) ##STR00076##

(90) To a mixed liquor of compound 6-3 (400.00 mg, 2.01 mmol, 1.00 eq), potassium hydroxide (225.52 mg, 4.02 mmol, 2.00 eq) and 2-di-tert-butylphosphine-2′,4′,6′-triisopropylbiphenyl (85.34 mg, 201.00 μmol, 0.10 eq) in 1,4-dioxane (10.00 mL) were added water (1.00 mL) and tris(dibenzylideneacetone) dipalladium (184.03 mg, 201.00 μmol, 0.10 eq). The mixture was reacted at 120° C. under the protection of nitrogen gas in a microwave instrument for 1 hour. The reaction mixture was directly concentrated under reduced pressure. The residue was dissolved in ethyl acetate (20 mL). The mixture was washed with water (10 mL) and a saturated saline solution (10 mL), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure. The compound as a crude product was purified with the preparative plate (petroleum ether/ethyl acetate=5/1) to give compound 6-4. .sup.1H NMR (400 MHz, CDCl.sub.3-d) δppm 7.15 (t, J=7.6 Hz, 1H), 6.80 (d, J=7.6 Hz, 1H), 6.66 (d, J=7.6 Hz, 1H), 5.15 (m, 4H).

(91) Synthesis of Compound 6-5

(92) ##STR00077##

(93) At −5° C. and under the protection of nitrogen gas, concentrated sulphuric acid (36.75 mg, 367.24 μmol, 19.97 μL, 98% purity, 1.00 eq) was added to a solution of compound 6-4 (50.00 mg, 367.24 μmol, 1.00 eq) dissolved in methylene chloride (2 mL). Then fuming nitric acid (24.36 mg, 367.24 μmol, 17.40 μL, 1.00 eq) (purity 95%) diluted in methylene chloride (0.5 mL) was slowly added to the reaction mixture. The resulting mixture was stirred for 0.5 hour. The reaction mixture was diluted with methylene chloride (10 mL), then washed with water (5 mL) and a saturated saline solution (5 mL), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure. The compound as a crude product was purified with a preparative plate (petroleum ether/ethyl acetate=3/1) to give compound 6-5. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.94 (d, J=8.8 Hz, 1H), 6.72 (d, J=8.8 Hz, 1H), 5.32 (s, 2H), 4.97 (s, 2H).

(94) Synthesis of Compound 6-6

(95) ##STR00078##

(96) Under the protection of nitrogen gas, to a solution of compound 6-5 (40.00 mg, 220.81 μmol, 1.00 eq) dissolved in methanol (10.00 mL) was added Pd/C (100.00 mg) (containing palladium 20%, water 50%). Then the atmosphere of the reaction system was replaced with hydrogen gas three times. The reaction mixture was reacted at 20° C. under a hydrogen balloon (15 psi) for 1 hour. The reaction mixture was directly filtered. The filter cake was washed with methanol (10 mL). The filtrate was directly concentrated under reduced pressure to give compound 6-6, which was directly used in the next step of the reaction. LCMS (ESI) m/z: 151.9 (M+1).

(97) Synthesis of Compounds 6 and 7

(98) ##STR00079##

(99) Compound 1-11 (50.00 mg, 124.73 μmol, 1.00 eq), compound 6-6 (30.00 mg, 198.32 μmol, 1.59 eq), triethylamine (37.86 mg, 374.19 μmol, 51.86 μL, 3.00 eq) and HATU (71.14 mg, 187.10 μmol, 1.50 eq) were dissolved in methylene chloride (5.00 mL). The mixture was stirred at 20° C. under the protection of nitrogen gas for 2 hours. The reaction mixture was diluted with methylene chloride (10 mL) and washed with water (10 mL) and a saturated saline solution (10 mL). The organic phase was dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure. The compound as a crude product was purified with a preparative plate to give compound 6. LCMS (ESI) m/z: 534.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.47 (s, 1H), 7.36 (d, J=8.0 Hz, 2H), 7.28 (d, J=8.4 Hz, 2H), 7.07 (d, J=8.4 Hz, 1H), 6.57 (d, J=8.0 Hz, 1H), 4.85-5.00 (m, 4H), 4.53-4.56 (m, 1H), 3.63-3.65 (m, 1H), 3.34-3.38 (m, 1H), 2.62 (s, 3H), 2.34 (s, 3H), 1.62 (s, 3H).

(100) Compound 7. LCMS (ESI) m/z: 534.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.65 (s, 1H), 8.57 (br s, 1H), 7.35 (d, J=8.4 Hz, 2H), 7.26 (d, J=8.4 Hz, 2H), 6.90 (d, J=8.0 Hz, 1H), 6.53 (d, J=8.8 Hz, 1H), 4.95 (s, 2H), 4.90 (d, J=12.8 Hz, 1H), 4.80 (d, J=12.8 Hz, 1H), 4.57-4.60 (m, 1H), 3.63-3.69 (m, 1H), 3.35-3.39 (m, 1H), 2.62 (s, 3H), 2.34 (s, 3H), 1.61 (s, 3H).

(101) ##STR00080##

Example 8

(102) Synthesis of Compound 8-2

(103) ##STR00081##

(104) Compound 8-1 (500.00 mg, 3.10 mmol, 1.00 eq) and hydrazine hydrate (4.12 g, 80.66 mmol, 4.00 mL, 26.02 eq) were added to a microwave tube. The mixture was reacted in the microwave at 90° C. for 1 hour. A large amount of solid precipitated from the reaction mixture. The reaction was terminated. The reaction mixture was filtered. The filter cake was washed with water (20 mL×2). Then anhydrous tetrahydrofuran (20 mL×2) was added. The mixture was concentrated under reduced pressure to give compound 8-2 without any further purification. LCMS (ESI) m/z: 161.9 (M+1).

(105) Synthesis of Compound 8

(106) ##STR00082##

(107) A solution of compound 1-11 (100.00 mg, 249.45 μmol, 1.00 eq) and compound 8-2 (100.50 mg, 623.63 μmol, 2.50 eq) in pyridine (5.00 mL) was added dropwisely to POCl.sub.3 (114.74 mg, 748.35 μmol, 69.54 μL, 3.00 eq). The mixture was reacted under being stirred at 20° C. for 12 hours. The reaction mixture was diluted with ethyl acetate (10 mL) and washed with water (5 mL×2) and a saturated saline solution (5 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The compound as a crude product was purified with a preparative chromatography to give compound 8. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 10.49-10.54 (m, 1H), 9.84 (br s, 1H), 8.36-8.37 (m, 1H), 7.79-7.98 (m, 1H), 7.72-7.74 (m, 2H), 7.46 (d, J=8.4 Hz, 2H), 7.34 (d, J=8.4 Hz, 2H), 4.70-4.73 (m, 1H), 3.73-3.86 (m, 2H), 2.68 (s, 3H), 2.40 (s, 3H), 1.67 (s, 3H). LCMS (ESI) m/z: 544.1 (M+1).

(108) ##STR00083##

Example 9

(109) ##STR00084##

(110) Synthesis of Compound 9-2

(111) ##STR00085##

(112) At −20° C. and under the protection of nitrogen gas, compound 9-1 (2.00 g, 15.02 mmol, 1.00 eq) and anhydrous diisopropylamine (3.16 g, 31.24 mmol, 4.39 mL, 2.08 eq) were dissolved in anhydrous tetrahydrofuran (30.00 mL). After the solution was cooled to −20° C., n-butyl lithium (2.5 M, 23.73 mL, 3.95 eq) was slowly added dropwisely, and the resulting mixture was maintained at a temperature between −20 and −30° C. After the completion of the dropwise addition, the mixture was warmed up to 0° C. and reacted under being stirred for 1 hour. Then a solution of 1,2-dibromoethane (9.62 g, 51.22 mmol, 3.86 mL, 3.41 eq) in anhydrous tetrahydrofuran (10.00 mL) was slowly added dropwisely. After the completion of the dropwise addition, the mixture was warmed up to 27° C. and reacted under being stirred for 18 hours. At −20° C., a saturated ammonium chloride solution (20 mL) was added to quench the reaction. The reaction mixture was adjusted with 3N hydrochloric acid (5 mL) to pH=2-3, and then extracted with ethyl acetate (3×20 mL). The above organic phases were combined and washed with a saturated sodium bicarbonate solution (50 mL) and a saturated saline solution (50 mL) successively. The obtained organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give a purple solid. The crude product was purified with a flash chromatography column to give compound 9-2. .sup.1H NMR (400 MHz, CDCl.sub.3): δppm 9.12 (s, 1H), 7.11 (m, 1H), 6.89-6.96 (m, 2H), 6.75 (d, J=7.28 Hz, 1H), 1.70 (t, J=4.0 Hz, 2H), 1.47 (t, J=4.0 Hz, 2H).

(113) Synthesis of Compound 9-3

(114) ##STR00086##

(115) At −15° C. and under the protection of nitrogen gas, nitric acid (118.46 mg, 1.88 mmol, 84.61 μL, 1.00 eq) was slowly added dropwisely to a solution of compound 9-2 (300.00 mg, 1.88 mmol, 1.00 eq) and concentrated sulphuric acid (184.85 mg, 1.88 mmol, 100.46 μL, 1.00 eq) in methylene chloride (4.00 mL). After the completion of the dropwise addition, the mixture was warmed up to 27° C. and the stirring was kept on for 10 hours. Ice (about 2 g) was added to the reaction mixture to quench the reaction. The reaction mixture was extracted with methylene chloride (3×5 mL). The above organic phases were combined. The organic phase was washed with a saturated sodium bicarbonate solution (10 mL) and a saturated saline solution (10 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The crude product was purified with a thin-layer chromatography plate to give compound 9-3. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.32 (s, 1H), 8.12 (m, 1H), 7.67 (d, J=2.0 Hz, 1H), 6.97 (d, J=8.4 Hz, 1H), 1.81-1.84 (m, 2H), 1.61-1.68 (m, 2H).

(116) Synthesis of Compound 9-4

(117) ##STR00087##

(118) Reduced iron powder (462.27 mg, 8.28 mmol, 13.00 eq) was added to a solution of compound 9-3 (130 mg, 636.69 μmol, 1.0 eq) in glacial acetic acid (8.00 mL). The mixture was stirred at 25° C. under the protection of nitrogen gas for 16 hours. The reaction mixture was filtered. The filtrate was concentrated and then added to water (5 mL). The aqueous phase was extracted with ethyl acetate (3×5 mL). The combined organic phases were washed with a saturated saline solution (10 mL), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The reaction mixture was filtered with diatomaceous earth. The filtrate was concentrated under reduced pressure. The obtained crude product was separated and purified with a thin-layer chromatography plate to give compound 9-4. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.37 (s, 1H), 6.75 (d, J=8.0 Hz, 1H), 6.48-6.63 (m, 1H), 6.23 (s, 1H), 1.64-1.82 (m, 2H), 1.40-1.51 (m, 2H).

(119) Synthesis of Compound 9

(120) ##STR00088##

(121) At 25° C. and under the protection of nitrogen gas, compound 1-11 (36.82 mg, 91.85 μmol, 1.00 eq) and compound 9-4 (16.00 mg, 91.85 μmol, 1.00 eq) were added to a solution of HATU (41.91 mg, 110.22 μmol, 1.20 eq) in anhydrous methylene chloride (4.00 mL), and then triethylamine (27.88 mg, 275.55 μmol, 38.19 μL, 3.00 eq) was slowly added dropwisely. The mixture was stirred at 25° C. under the protection of nitrogen gas for 5 hours. Water (3 mL) was added to the reaction mixture to quench the reaction. The mixture was separated into two phases. The aqueous phase was extracted with methylene chloride (3×5 mL). The above organic phases were combined. The organic phase was washed with a saturated sodium bicarbonate solution (2 mL) and a saturated saline solution (2 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The obtained crude product was purified with a preparative chromatography (basicity) to give compound 9. .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 9.19 (s, 1H), 8.64 (s, 1H), 7.31-7.37 (m, 2H), 7.23-7.27 (m, 2H), 7.19 (s, 1H), 7.08-7.11 (m, 1H), 6.76 (d, J=8.4 Hz, 1H), 4.60-4.64 (m, 1H), 3.73-3.79 (m, 1H), 3.39-3.44 (m, 1H), 2.61 (s, 3H), 2.34 (s, 3H), 1.62-1.64 (m, 2H), 1.61 (s, 3H), 1.42-1.43 (m, 2H). LCMS (ESI) m/z: 557.1 (M+1).

(122) ##STR00089##

Example 10

(123) ##STR00090##

(124) Synthesis of Compound 10-2

(125) ##STR00091##

(126) A mixed liquor of compound 10-1 (1.76 g, 6.77 mmol, 1.00 eq), cuprous cyanide (910.00 mg, 10.16 mmol, 2.22 mL, 1.50 eq), dppf (375.32 mg, 677.00 μmol, 0.10 eq), bis(dibenzylideneacetone) palladium (389.28 mg, 677.00 μmol, 0.10 eq) and N,N-dimethyl formamide (20.00 mL) was heated to 110° C. and stirred for 16 hours. The reaction mixture was filtered under reduced pressure. The filtrate was concentrated under reduced pressure. The concentrated residue was purified with a silica gel column (petroleum ether/ethyl acetate=1/0-3/1) to give compound 10-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 8.98 (d, J=2.3 Hz, 1H), 8.52 (dd, 8.5 Hz, 1H), 8.05 (d, J=8.3 Hz, 1H), 4.09 (s, 3H).

(127) Synthesis of Compound 10-3

(128) ##STR00092##

(129) A solution of compound 10-2 (700.00 mg, 3.40 mmol, 1.00 eq) in glacial acetic acid (10.00 mL) was added to reduced iron powder (1.90 g, 34.00 mmol, 10.00 eq). The obtained reaction mixture was stirred at 20° C. for 16 hours. The reaction mixture was filtered with diatomaceous earth, and concentrated under reduced pressure. To the concentrated residue were added ethyl acetate (100 mL) and a saturated aqueous sodium bicarbonate solution (pH 7-8). The organic phase was washed with a saturated saline solution (60 mL), dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The concentrated residue was purified with a silica gel column (petroleum ether/ethyl acetate=1/0-1/1) to give compound 10-3. .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 7.47 (d, J=8.3 Hz, 1H), 7.26 (d, J=2.5 Hz, 1H), 6.73 (dd, J=2.5, 8.3 Hz, 1H), 4.21 (br s, 2H), 3.90 (s, 3H).

(130) Synthesis of Compound 10-4

(131) ##STR00093##

(132) At −78° C., to a solution of compound 10-3 (100.00 mg, 567.63 μmol, 1.00 eq) and Ti(i-PrO).sub.4 (643.20 mg, 2.26 mmol, 670.00 μL, 3.99 eq) in tetrahydrofuran (2.00 mL) was added magnesium ethyl bromide (3 M, 1.50 mL, 7.93 eq). The obtained reaction mixture was stirred at a temperature between −78° C. and 10° C. (being warmed up slowly) for 18 hours. A saturated ammonium chloride solution (20 mL) was added to the reaction mixture to form a viscous slurry. Ethyl acetate (20 mL) was added. The resulting mixture was stirred for 10 minutes and then filtered. The filtrate was separated into two phases. The organic phase was washed with a saturated saline solution (15 mL), dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The concentrated residue was purified with a thin-layer chromatography plate to give compound 10-4. LCMS: MS (ESI) m/z: 174.9 (M+1).

(133) Synthesis of Compound 10

(134) ##STR00094##

(135) Compound 1-11 (25.00 mg, 62.36 μmol, 1.00 eq), compound 10-4 (11.95 mg, 68.60 μmol, 1.10 eq), HATU (28.45 mg, 74.84 μmol, 1.20 eq) and triethylamine (15.78 mg, 155.91 μmol, 21.61 μL, 2.50 eq) were dissolved in anhydrous methylene chloride (1.00 mL). The mixture was stirred at 20° C. under the protection of nitrogen gas for 2 hours. The reaction mixture was diluted with methylene chloride (10 mL) and washed with water (5 mL) and a saturated saline solution (5 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The compound as a crude product was purified with a preparative chromatography to give compound 10. .sup.1HNMR (400 MHz, CDCl.sub.3) δ ppm 8.10 (br s, 1H), 7.94 (dd, 8.4 Hz, 1H), 7.43 (d, J=8.4 Hz, 2H), 7.35 (d, J=8.8 Hz, 2H), 6.96 (d, J=8.0 Hz, 1H), 6.83 (s, 1H), 4.72-4.76 (m, 1H), 3.86-3.92 (m, 1H), 3.61-3.66 (m, 1H), 2.71 (s, 3H), 2.43 (s, 3H), 1.71 (s, 3H), 1.54-1.57 (m, 2H), 1.35-1.50 (m, 2H). LCMS (ESI) m/z: 557.1 (M+1).

(136) ##STR00095##

Example 11

(137) ##STR00096##

(138) Synthesis of Compound 11-2

(139) ##STR00097##

(140) A mixture of ortho-xylene (5.00 g, 47.09 mmol, 5.68 mL, 1.00 eq), NBS (17.60 g, 98.89 mmol, 2.10 eq), benzoyl peroxide (228.13 mg, 941.80 μmol, 0.02 eq) and chloroform (50.00 mL) was stirred at 80° C. for 5 hours. The reaction mixture was cooled to room temperature, then diluted with methylene chloride (100 mL), washed with water (80 mL×2), and washed with a saturated saline solution (50 mL). The organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The resulting solid was slurried with (petroleum ether/ethanol=30:1; 60 mL/2 mL) at 80° C. for 20 minutes, cooled to room temperature, and filtered. The filter cake was washed with petroleum ether (20 mL). The filter cake was oven-dried to give compound 11-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.40-7.36 (m, 2H), 7.34-7.30 (m, 2H), 4.68 (s, 4H).

(141) Synthesis of Compound 11-3

(142) ##STR00098##

(143) A mixture of compound 11-2 (6.00 g, 22.73 mmol, 3.06 mL, 1.00 eq), sodium sulfide nonahydrate (16.38 g, 68.19 mmol, 11.45 mL, 3.00 eq), benzyl triethyl ammonium chloride (258.86 mg, 1.14 mmol, 0.05 eq), methylene chloride (60.00 mL) and water (60.00 mL) was stirred at 18° C. in the dark for 24 hours. The mixture was extracted with methylene chloride (100 mL). The organic phase was washed with water (80 mL×5), washed with a saturated saline solution (50 mL), then dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give compound 11-3, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.20-7.14 (m, 4H), 4.21 (s, 4H).

(144) Synthesis of Compound 11-4

(145) ##STR00099##

(146) Compound 11-3 (2.80 g, 20.56 mmol, 1.00 eq) was dissolved in glacial acetic acid (15.00 mL) at 5-10° C., and then hydrogen peroxide (5.90 g, 52.02 mmol, 5.00 mL, 30% purity, 2.53 eq) was added dropwisely. After the completion of the dropwise addition, the mixture was stirred at 20° C. for 1 hour, and then warmed up to 90° C. and stirred for 3 hours. The reaction mixture was cooled and a solid precipitated. After filtration, the filter cake was washed with water (20 mL). The filter cake was slurried with ethanol (20 mL) at 80° C. for 20 minutes, and the resulting mixture was filtered. The filter cake was oven-dried (1 g). The filtrate was placed for 24 hours and a solid precipitated. After filtration, the filter cake was oven-dried (1 g). The filter cakes were combined to give compound 11-4, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.41-7.35 (m, 2H), 7.35-7.29 (m, 2H), 4.39 (s, 4H).

(147) Synthesis of Compound 11-5

(148) ##STR00100##

(149) Compound 11-4 (300.00 mg, 1.78 mmol, 1.00 eq) was dissolved in concentrated sulphuric acid (2.00 mL) at −10° C., and then potassium nitrate (180.31 mg, 1.78 mmol, 1.00 eq) was added. The mixture was stirred at −10° C. for 5 minutes. At −10° C., ice cubes (20 g) were added to quench the reaction. The ice cubes melted and a solid precipitated. After filtration, the filter cake was washed with water (10 mL). The filter cake was oven-dried to give compound 11-5, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δppm 8.30 (s, 1H), 8.24 (dd, J=2.0, 8.5 Hz, 1H), 7.69 (d, J=8.5 Hz, 1H), 4.67 (d, J=8.3 Hz, 4H).

(150) Synthesis of Compound 11-6

(151) ##STR00101##

(152) Compound 11-5 (200.00 mg, 938.04 μmol, 1.00 eq) and stannous chloride dihydrate (846.68 mg, 3.75 mmol, 312.43 μL, 4.00 eq) were dissolved in ethanol (3.00 mL), and then concentrated hydrochloric acid (1.32 g, 5.85 mmol, 1.20 mL, 37% purity, 6.23 eq) was added dropwisely at 15° C. After the completion of the dropwise addition, the mixture was stirred at 80° C. for 1 hour, adjusted with a NaOH solution (2N) to pH=10. The mixture was concentrated under reduced pressure to about 50 mL and then extracted with (methylene chloride/methanol=10:1) (40 mL×6). The combined organic phases were washed with a saturated saline solution (50 mL×2). The organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 11-6, which was directly used in the next step without any further purification. LCMS (ESI) m/z: 184.1, 206.0 (M+1), (M+23). .sup.1H NMR (400 MHz, DMSO-d.sub.6) δppm 6.98 (d, J=8.3 Hz, 1H), 6.56-6.52 (m, 1H), 6.50 (s, 1H), 5.31 (br s, 2H), 4.30 (s, 2H), 4.24 (s, 2H).

(153) Synthesis of Compound 11

(154) ##STR00102##

(155) At 15° C. and under the protection of nitrogen gas, compound 1-11 (1.00 g, 2.49 mmol, 1.00 eq) and compound 11-6 (547.49 mg, 2.99 mmol, 1.20 eq) were dissolved in anhydrous N,N-dimethyl formamide (15.00 mL). HATU (946.77 mg, 2.49 mmol, 1.00 eq) was added and diisopropylethylamine (965.42 mg, 7.47 mmol, 1.30 mL, 3.00 eq) was added dropwisely. The mixture was stirred at 15° C. in a nitrogen atmosphere for 1 hour. The reaction mixture was directly dried. The obtained solid was shaking washed with water (40 mL). The mixture was extracted with methylene chloride (30 mL×2). The organic phase was washed with a saturated saline solution (50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a flash chromatography column to give compound 11. LCMS (ESI) m/z: 567.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 9.68 (s, 1H), 7.57 (s, 1H), 7.22-7.37 (m, 5H), 7.02 (d, J=8.8 Hz, 1H), 4.60-4.63 (m, 1H), 4.17-4.21 (m, 4H), 3.82-3.88 (m, 1H), 3.38-3.43 (m, 1H), 2.62 (s, 3H), 2.35 (s, 3H), 1.63 (s, 3H).

(156) ##STR00103##

Example 12

(157) ##STR00104##

(158) Synthesis of Compound 12-2

(159) ##STR00105##

(160) A mixed liquor of compound 12-1 (2.00 g, 7.69 mmol, 1.00 eq), CuCN (1.04 g, 11.61 mmol, 2.54 mL, 1.51 eq), dppf (426.38 mg, 769.00 μmol, 0.10 eq), tris(dibenzylideneacetone) dipalladium (442.25 mg, 769.00 μmol, 0.10 eq) and N,N-dimethyl formamide (20.00 mL) was heated to 120° C. and stirred for 16 hours. The reaction mixture was filtered with diatomaceous earth. The filtrate was concentrated under reduced pressure. The concentrated residue was purified with a silica gel column (petroleum ether/ethyl acetate=1/0-1/1) to give compound 12-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.65 (d, J=2.3 Hz, 1H), 8.51 (dd, 8.8 Hz, 1H), 8.36 (d, J=8.5 Hz, 1H), 4.08 (s, 3H).

(161) Synthesis of Compound 12-3

(162) ##STR00106##

(163) To a solution of compound 12-2 (900.00 mg, 4.37 mmol, 1.00 eq) in glacial acetic acid (20.00 mL) was added reduced iron powder (2.44 g, 43.70 mmol, 10.00 eq). The obtained reaction mixture was stirred at 20° C. for 4 hours. The reaction mixture was filtered with diatomaceous earth, and concentrated under reduced pressure. To the concentrated residue were added ethyl acetate (150 mL) and a saturated aqueous sodium bicarbonate solution (pH 7-8). The organic phase was washed with a saturated saline solution (100 mL), dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The concentrated residue was purified with a thin-layer chromatography plate to give compound 12-3. LCMS (ESI) m/z: 177.1 (M+1).

(164) Synthesis of Compound 12-4

(165) ##STR00107##

(166) At −78° C., to a solution of compound 12-3 (490.00 mg, 2.78 mmol, 1.00 eq) and Ti(i-PrO).sub.4 (3.07 g, 10.81 mmol, 3.20 mL, 3.89 eq) in tetrahydrofuran (15.00 mL) was added magnesium ethyl bromide (3 M, 7.40 mL, 7.99 eq). The obtained reaction mixture was stirred at a temperature between −78° C. and 15° C. (being warmed up slowly) for 16 hours. As the reaction proceeded, a yellow solid precipitated, and the reaction mixture gradually became earthy yellow and viscous. To the reaction mixture was added a saturated ammonium chloride solution (30 mL), and a viscous slurry was formed. Ethyl acetate (30 mL) was added. The mixture was stirred for 10 minutes and then filtered with diatomaceous earth. The filtrate was separated into two phases. The organic phase was washed with a saturated saline solution (30 mL), dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The concentrated residue was purified with a thin-layer chromatography plate to give compound 12-4. LCMS (ESI) m/z: 174.9 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.65 (d, J=8.0 Hz, 1H), 6.68 (dd, J=2.0, 8.3 Hz, 1H), 6.43 (br s, 1H), 6.23 (d, J=1.8 Hz, 1H), 4.04 (br s, 2H), 1.52-1.46 (m, 2H), 1.37-1.32 (m, 2H).

(167) Synthesis of Compound 12

(168) ##STR00108##

(169) Compound 12-4 (15.64 mg, 89.80 μmol, 1.20 eq) and diisopropylethylamine (29.02 mg, 224.51 μmol, 39.21 μL, 3.00 eq) were dissolved in anhydrous N,N-dimethyl formamide (3.00 mL). Compound 1-11 (30.00 mg, 74.84 μmol, 1.00 eq) and HATU (28.45 mg, 74.84 μmol, 1.00 eq) were added. The atmosphere was replaced with nitrogen gas three times. The mixture was stirred at 15° C. in a nitrogen atmosphere for 16 hours. The reaction mixture was directly concentrated under reduced pressure. The residue was dissolved in methylene chloride (15 mL) and then shaking washed with water (10 mL). The organic phase was washed with a saturated saline solution (20 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a preparative chromatography to give compound 12. LCMS (ESI) m/z: 557.2 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 9.91 (br s, 1H), 7.68 (d, J=8.0 Hz, 1H), 7.63 (s, 1H), 7.44 (d, J=8.8 Hz, 2H), 7.35 (d, J=8.8 Hz, 2H), 7.22 (d, J=8.0 Hz, 1H), 4.70-4.74 (m, 1H), 3.95-3.99 (m, 1H), 3.50-3.54 (m, 1H), 2.73 (s, 3H), 2.45 (s, 3H), 1.72 (s, 3H), 1.46-1.51 (m, 2H), 1.39-1.41 (m, 2H).

(170) ##STR00109##

Example 13

(171) ##STR00110##

(172) Synthesis of Compound 13-1

(173) ##STR00111##

(174) Compound 11-3 (1.80 g, 13.21 mmol, 1.00 eq) was dissolved in methanol (15.00 mL), and then a solution of sodium periodate (2.83 g, 13.21 mmol, 732.26 μL, 1.00 eq) in H.sub.2O (15.00 mL) was added dropwisely. After the completion of the dropwise addition, the mixture was stirred at 15° C. for 12 hours. The reaction mixture was concentrated under reduced pressure to about 10 mL and extracted with ethyl acetate (20 mL×5). The combined organic phases were washed with a saturated saline solution (40 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 13-1, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.40-7.36 (m, 2H), 7.36-7.31 (m, 2H), 4.34-4.27 (m, 2H), 4.22-4.13 (m, 2H).

(175) Synthesis of Compound 13-2

(176) ##STR00112##

(177) Compound 13-1 (600.00 mg, 3.94 mmol, 1.00 eq) was dissolved in concentrated sulphuric acid (5.00 mL) at −10° C., and then potassium nitrate (398.53 mg, 3.94 mmol, 1.00 eq) was added. The mixture was stirred at −10° C. for 5 minutes. At −10° C., ice cubes (20 g) were added to quench the reaction. The ice cubes melted, and the mixture was extracted with (methylene chloride/methanol=10:1) (30 mL×4). The combined organic phases were washed with a saturated saline solution (40 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 13-2, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δppm 8.30 (s, 1H), 8.24 (dd, J=2.0, 8.5 Hz, 1H), 7.69 (d, J=8.5 Hz, 1H), 4.67 (d, J=8.3 Hz, 4H).

(178) Synthesis of Compound 13-3

(179) ##STR00113##

(180) Compound 13-2 (300.00 mg, 1.52 mmol, 1.00 eq) was dissolved in ethanol (8.00 mL), and then stannous chloride dihydrate (686.53 mg, 3.04 mmol, 253.33 μL, 2.00 eq) was added. The mixture was stirred at 80° C. for 1 hour. The mixture was adjusted with a sodium hydroxide solution (1 N) to pH=10, concentrated under reduced pressure to about 50 mL, then extracted with (methylene chloride/methanol=10:1) (40 mL×4). The combined organic phases were washed with a saturated saline solution (60 mL). The organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The crude product was purified with a thin-layer chromatography plate to give compound 13-3. LCMS (ESI) m/z: 167.8 (M+1).

(181) Synthesis of Compound 13

(182) ##STR00114##

(183) Compound 1-11 (60.00 mg, 149.67 μmol, 1.00 eq) and compound 13-3 (30.04 mg, 179.60 μmol, 1.20 eq) were successively added to a solution of HATU (74.00 mg, 194.62 μmol, 1.30 eq) in anhydrous N,N-dimethyl formamide (3.00 mL), and then diisopropylethylamine (59.20 mg, 458.06 μmol, 80.00 μL, 3.06 eq) was slowly added dropwisely. The mixture was stirred at 15° C. under the protection of nitrogen gas for 6 hours. Water (3 mL) was added to the reaction mixture to quench the reaction. The mixture was extracted with methylene chloride (3×5 mL). The above organic phases were combined. The organic phase was washed with a saturated sodium bicarbonate solution (5 mL) and a saturated saline solution (5 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The obtained crude product was purified with a preparative chromatography to give compound 13. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.35-9.73 (m, 1H), 7.54-7.64 (m, 1H), 7.22-7.38 (m, 5H), 7.09-7.23 (m, 1H), 4.62-4.64 (m, 1H), 3.94-4.18 (m, 4H), 3.81-3.93 (m, 1H), 3.40-3.50 (m, 1H), 2.62 (s, 3H), 2.34 (s, 3H), 1.62 (s, 3H). LCMS (ESI) m/z: 550.1 (M+1).

(184) ##STR00115##

Examples 14 and 15

(185) ##STR00116##

(186) Compound 13 (38 mg, 69.08 μmol) was subjected to a SFC separation (chromatography column: AD (250 mm×30 mm, 10 μm); mobile phase: [0.1% NH.sub.3H.sub.2O EtOH]; B %: 55%-55%, 50 mL/min) to give compound 14 (Rt=0.837 min). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.49 (s, 1H), 7.59 (s, 1H), 7.25-7.38 (m, 5H), 7.12 (d, J=8.0 Hz, 1H), 4.61-4.64 (m, 1H), 3.99-4.18 (m, 4H), 3.74-3.77 (m, 1H), 3.44-3.50 (m, 1H), 2.62 (s, 3H), 2.34 (s, 3H), 1.61 (s, 3H). LCMS (ESI) m/z: 550.0 (M+1).

(187) Compound 15 (Rt=1.666 min). .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 9.73 (s, 1H), 7.54 (s, 1H), 7.22-7.38 (m, 5H), 7.03 (d, J=8.8 Hz, 1H), 4.65-4.68 (m, 1H), 4.06-4.13 (m, 2H), 3.88-3.98 (m, 3H), 3.40-3.50 (m, 1H), 2.62 (s, 3H), 2.34 (s, 3H), 1.62 (s, 3H). LCMS (ESI) m/z: 550.0 (M+1).

(188) ##STR00117##

Example 16

(189) ##STR00118##

(190) Compound 1-11 (60.00 mg, 149.67 μmol, 1.00 eq) and compound 16-1 (24.28 mg, 179.60 μmol, 1.20 eq) were dissolved in anhydrous methylene chloride (5.00 mL), HATU (56.91 mg, 149.67 μmol, 1.00 eq) was added, and diisopropylethylamine (58.03 mg, 449.01 μmol, 78.42 μL, 3.00 eq) was added dropwisely. The mixture was stirred at 15° C. in a nitrogen atmosphere for 1 hour. The reaction mixture was shaking washed with water (10 mL). The organic phase was washed with a saturated saline solution (20 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a preparative chromatography to give compound 16.

(191) LCMS (ESI) m/z: 518.0 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.88 (s, 1H), 7.62 (s, 1H), 7.44 (d, J=8.8 Hz, 2H), 7.36 (d, J=8.8 Hz, 2H), 7.32-7.34 (m, 1H), 7.16 (d, J=8.0 Hz, 1H), 5.08 (s, 4H), 4.62-4.65 (m, 1H), 3.79-3.85 (m, 1H), 3.48-3.53 (m, 1H), 2.71 (s, 3H), 2.43 (s, 3H), 1.71 (s, 3H).

(192) ##STR00119##

Example 17

(193) ##STR00120##

(194) Synthesis of Compound 17-2

(195) ##STR00121##

(196) At −20° C. and under the protection of nitrogen gas, concentrated sulphuric acid (16.00 mL) was slowly added to a solution of compound 17-1 (3.90 g, 32.73 mmol, 3.71 mL, 1.00 eq) dissolved in methylene chloride (10.00 mL). After the completion of the dropwise addition, the mixture was slowly warmed up to 20° C. Then methylene chloride was removed under reduced pressure to give a light brown solution. Concentrated nitric acid (5.60 g, 62.19 mmol, 4.00 mL, 1.90 eq) (the content of about 70%) was slowly added dropwisely to the above light brown solution, ensuring the inner temperature not exceeding 20° C. After the completion of the dropwise addition, the mixture was stirred for 0.5 hour. The reaction mixture was slowly added to ice water (300 mL) and then adjusted with solid sodium bicarbonate to pH=8. Methyl tert-butyl ether (300 mL) was added. The mixture was stirred for 1 hour. The organic phase was separated. The aqueous phase was extracted with methyl tert-butyl ether (200 mL×2). The combined organic phases were washed with a saturated saline solution (300 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give compound 17-2, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.05 (dd, J=2.0, 8.0 Hz, 1H), 8.03 (s, 1H), 7.30 (d, J=8.0 Hz, 1H), 4.24 (s, 4H).

(197) Synthesis of Compound 17-3

(198) ##STR00122##

(199) At 0° C., to a solution of compound 17-2 (150.00 mg, 913.74 μmol, 1.00 eq) and triethylamine (277.38 mg, 2.74 mmol, 379.98 μL, 3.00 eq) in anhydrous methylene chloride (5.00 mL) was added acetyl chloride (71.73 mg, 913.74 μmol, 65.21 μL, 1.00 eq). The mixture was reacted under being stirred at 20° C. for 2 hours. The reaction mixture was directly concentrated under reduced pressure. The crude product was purified with a thin-layer chromatography plate to give compound 17-3. .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 8.08-8.15 (m, 2H), 7.35-7.41 (m, 1H), 4.84 (s, 2H), 4.81 (s, 2H), 2.13 (s, 3H).

(200) Synthesis of Compound 17-4

(201) ##STR00123##

(202) Wet Pd/C (100.00 mg, 10% Pd) was added to a solution of compound 17-3 (140.00 mg, 678.95 μmol, 1.00 eq) in methanol (3.00 mL). The atmosphere was replaced with hydrogen gas three times. The mixture was stirred at 15° C. at a hydrogen balloon (15 psi) condition for 18 hours. The reaction mixture was filtered with diatomaceous earth. The filtrate was directly concentrated under reduced pressure to give compound 17-4, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.01-7.07 (m, 1H), 6.57-6.65 (m, 2H), 4.67-4.73 (m, 4H), 2.15 (s, 3H).

(203) Synthesis of Compound 17

(204) ##STR00124##

(205) Compound 1-11 (200.00 mg, 498.90 μmol, 1.00 eq) and compound 17-4 (100.00 mg, 567.50 μmol, 1.14 eq) were successively added to a solution of HATU (240.00 mg, 631.20 μmol, 1.27 eq) in anhydrous N,N-dimethyl formamide (5.00 mL), and then triethylamine (146.00 mg, 1.44 mmol, 200.00 μL, 2.89 eq) was slowly added dropwisely. The mixture was stirred at 15° C. under the protection of nitrogen gas for 4 hours. Water (5 mL) was added to the reaction mixture to quench the reaction. The mixture was separated into two phases. The aqueous phase was extracted with methylene chloride (3×5 mL). The above organic phases were combined. The organic phase was washed with a saturated sodium bicarbonate solution (5 mL) and a saturated saline solution (5 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a preparative chromatography to give compound 17. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.16-9.20 (m, 1H), 7.61-7.70 (m, 1H), 7.31-7.43 (m, 5H), 7.14-7.18 (m, 1H), 4.62-4.76 (m, 5H), 3.81-3.84 (m, 1H), 3.47-3.52 (m, 1H), 2.69 (s, 3H), 2.41 (s, 3H), 2.15 (s, 3H), 1.69 (s, 3H). LCMS (ESI) m/z: 559.1 (M+1).

(206) ##STR00125##

Example 18

(207) ##STR00126##

(208) Synthesis of Compound 18-1

(209) ##STR00127##

(210) Compound 17-2 (30.00 mg, 182.75 μmol, 1.00 eq) and (Boc).sub.2O (47.50 mg, 217.47 μmol, 50.00 μL, 1.19 eq) were successively added to a solution of 4-dimethylaminopyridine (1.00 mg, 8.19 μmol, 0.04 eq) in anhydrous methylene chloride (3.00 mL). Then to the reaction mixture was slowly added dropwisely triethylamine (55.48 mg, 548.25 μmol, 76.00 μL, 3.00 eq). The mixture was stirred at 15° C. under the protection of nitrogen gas for 6 hours. Water (3 mL) was added to the reaction mixture to quench the reaction. The mixture was separated into two phases. The aqueous phase was extracted with methylene chloride (3×5 mL). The above organic phases were combined. The organic phase was washed with a saturated sodium bicarbonate solution (5 mL) and a saturated saline solution (5 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 18-1, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.00-8.15 (m, 2H), 7.28-7.38 (m, 1H), 4.68 (d, J=10.8 Hz, 4H), 1.45 (s, 9H).

(211) Synthesis of Compound 18-2

(212) ##STR00128##

(213) Wet Pd/C (100.00 mg, 10% Pd) was added to a solution of compound 18-1 (40.00 mg, 151.35 μmol, 1.00 eq) in methanol (3.00 mL). The atmosphere was replaced with hydrogen gas three times. The mixture was stirred at 15° C. at a hydrogen balloon (15 psi) condition for 3 hours. The reaction mixture was filtered with diatomaceous earth. The filtrate was directly concentrated under reduced pressure to give compound 18-2, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 6.86-6.99 (m, 1H), 6.44-6.56 (m, 2H), 4.41-4.53 (m, 4H), 1.43 (s, 9H).

(214) Synthesis of Compound 18-3

(215) ##STR00129##

(216) Compound 1-11 (40.00 mg, 99.78 μmol, 1.00 eq) and compound 18-2 (30.00 mg, 128.05 μmol, 1.28 eq) were successively added to a solution of HATU (48.00 mg, 126.24 μmol, 1.27 eq) in anhydrous N,N-dimethyl formamide (3.00 mL), and then triethylamine (29.20 mg, 288.57 μmol, 40.00 μL, 2.89 eq) was slowly added dropwisely. The mixture was stirred at 15° C. under the protection of nitrogen gas for 4 hours. Water (3 mL) was added to the reaction mixture to quench the reaction. The mixture was separated into two phases. The aqueous phase was extracted with methylene chloride (3×5 mL). The above organic phases were combined. The organic phase was washed with a saturated sodium bicarbonate solution (5 mL) and a saturated saline solution (5 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a preparative chromatography to give compound 18-3. LCMS (ESI) m/z: 617.0 (M+1).

(217) Synthesis of Compound 18

(218) ##STR00130##

(219) At 15° C. and under the protection of nitrogen gas, trifluoroacetic acid (3.08 g, 27.01 mmol, 2.00 mL, 333.41 eq) was added to a solution of compound 18-3 (50.00 mg, 81.02 μmol, 1.00 eq) in anhydrous methylene chloride (6.00 mL). The mixture was stirred at 15° C. for 4 hours. The reaction mixture was directly concentrated under reduced pressure, adjusted with a saturated aqueous sodium bicarbonate solution to pH=7, and extracted with methylene chloride (3×5 mL). The above organic phases were combined. The organic phase was washed with a saturated saline solution (5 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a preparative chromatography to give compound 18. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.12 (s, 1H), 7.57 (s, 1H), 7.32-7.47 (m, 5H), 7.13-7.15 (m, 1H), 4.68-4.71 (m, 1H), 3.82-3.88 (m, 1H), 3.50-3.55 (m, 1H), 2.71 (s, 3H), 2.43 (s, 3H), 1.70 (s, 3H). LCMS (ESI) m/z: 539.1 (M+1).

(220) ##STR00131##

Example 19

(221) ##STR00132##

(222) Synthesis of Compound 19-1

(223) ##STR00133##

(224) Triethylamine (948.78 mg, 9.38 mmol, 1.30 mL, 2.96 eq) was added to a solution of compound 17-2 (520.00 mg, 3.17 mmol, 1.00 eq) in methylene chloride (5.00 mL). The mixture was cooled to 0° C. under the protection of nitrogen gas. To the reaction mixture was slowly added dropwisely methylsulfonyl chloride (370.00 mg, 3.23 mmol, 250.00 μL, 1.02 eq). After the completion of the dropwise addition, the mixture was warmed up to 15° C. and stirred for 2 hours.

(225) The reaction mixture was directly concentrated under reduced pressure. The above crude product was purified with a flash chromatography column to give compound 19-1. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.24 (d, J=8.4 Hz, 1H), 8.17 (s, 1H), 7.46 (d, J=8.4 Hz, 1H), 4.83 (s, 4H), 2.97 (s, 3H).

(226) Synthesis of Compound 19-2

(227) ##STR00134##

(228) Wet Pd/C (100.00 mg, 10% Pd) was added to a solution of compound 19-1 (50.00 mg, 206.40 μmol, 1.00 eq) in methanol (3.00 mL). The atmosphere was replaced with hydrogen gas three times. The mixture was stirred at 15° C. at a hydrogen balloon (15 psi) condition for 3 hours. The reaction mixture was filtered with diatomaceous earth. The filtrate was directly concentrated under reduced pressure to give compound 19-2, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 6.95 (d, J=8.28 Hz, 1H), 6.54-6.57 (m, 1H), 6.50 (s, 1H), 4.52 (s, 4H), 2.78 (s, 3H).

(229) Synthesis of Compound 19

(230) ##STR00135##

(231) Compound 1-11 (60.00 mg, 149.67 μmol, 1.00 eq) and compound 19-2 (40.00 mg, 188.58 μmol, 1.26 eq) were successively added to a solution of HATU (73.00 mg, 191.99 μmol, 1.28 eq) in anhydrous methylene chloride (3.00 mL), and then triethylamine (43.80 mg, 432.55 μmol, 60.00 μL, 2.89 eq) was slowly added dropwisely. The mixture was stirred at 15° C. under the protection of nitrogen gas for 2 hours. Water (3 mL) was added to the reaction mixture to quench the reaction. The mixture was separated into two phases. The aqueous phase was extracted with methylene chloride (3×5 mL). The above organic phases were combined. The organic phase was washed with a saturated sodium bicarbonate solution (5 mL) and a saturated saline solution (5 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product purified with a thin-layer chromatography plate to give compound 19. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.82 (s, 1H), 7.51 (s, 1H), 7.17-7.40 (m, 5H) 6.96-6.98 (m, 1H), 4.68-4.70 (m, 1H), 4.49 (d, J=17.2 Hz, 4H), 3.83-3.89 (m, 1H), 3.46-3.51 (m, 1H), 2.82 (s, 3H), 2.62 (s, 3H), 2.35 (s, 3H), 1.62 (s, 3H). LCMS (ESI) m/z: 595 (M+1).

(232) ##STR00136##

Example 20

(233) ##STR00137##

(234) Synthesis of Compound 20

(235) ##STR00138##

(236) Compound 18 (140.00 mg, 270.77 μmol, 1.00 eq) was added to a mixed liquor of glacial acetic acid (2.00 mL) and H.sub.2O (2 mL). Then to the reaction mixture was slowly added dropwisely a solution of sodium cyanate (35.00 mg, 538.38 μmol, 1.99 eq) in water (2 mL). The mixture was stirred at 15° C. for 18 hours. The reaction mixture was directly concentrated under reduced pressure. The crude product was purified with a preparative chromatography to give compound 20. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.09 (s, 1H), 7.58 (s, 1H), 7.24-7.37 (m, 5H), 7.08 (d, J=8.4 Hz, 1H), 4.50-4.58 (m, 5H), 4.42 (br s, 2H), 3.73-3.79 (m, 1H), 3.39-3.44 (m, 1H), 2.62 (s, 3H), 2.34 (s, 3H), 1.62 (s, 3H). LCMS (ESI) m/z: 560.0 (M+1).

(237) ##STR00139##

Example 21

(238) ##STR00140##

(239) Synthesis of Compound 21-2

(240) ##STR00141##

(241) Compound 21-1 (300.00 mg, 1.69 mmol, 1.00 eq) and diethylaminosulfur trifluoride (545.91 mg, 3.39 mmol, 447.47 μL, 2.00 eq) were dissolved in anhydrous methylene chloride (10.00 mL). The mixture was stirred at 15° C. in a nitrogen atmosphere for 16 hours. To the reaction mixture was added water (30 mL). Then, the mixture was extracted with methylene chloride (20 mL×2). The organic phase was washed with a saturated saline solution (30 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a thin-layer chromatography plate to give compound 21-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 8.13-8.17 (m, 2H), 7.39-7.42 (m, 1H), 3.51-3.59 (m, 4H).

(242) Synthesis of Compound 21-3

(243) ##STR00142##

(244) Compound 21-2 (70.00 mg, 351.49 μmol, 1.00 eq) and Pd(OH).sub.2/C (50 mg, 10% purity) were added to methanol (5.00 mL). The mixture was stirred at 15° C. under a hydrogen balloon atmosphere for 16 hours. The reaction mixture was filtered through a pad of celite and concentrated under reduced pressure to give compound 21-3, which was directly used in the next step without the need for any further purification.

(245) Synthesis of Compound 21

(246) ##STR00143##

(247) Compound 1-11 (60.00 mg, 149.67 μmol, 1.00 eq) and compound 21-3 (30.38 mg, 179.60 μmol, 1.20 eq) were added to anhydrous methylene chloride (5.00 mL), then HATU (56.91 mg, 149.67 μmol, 1.00 eq) was added, and diisopropylethylamine (58.03 mg, 449.01 μmol, 78.42 μL, 3.00 eq) was added dropwisely. The atmosphere was replaced with nitrogen gas three times. The mixture was stirred at 15° C. in a nitrogen atmosphere for 16 hours. The reaction mixture was directly concentrated under reduced pressure, then shaking washed with water (10 mL), and extracted with ethyl acetate (10 mL×2). The organic phase was washed with a saturated saline solution (20 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a preparative chromatography to give compound 21. LCMS (ESI) m/z: 551.9 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 9.00 (s, 1H), 7.60 (s, 1H), 7.43 (d, J=8.4 Hz, 2H), 7.33-7.38 (m, 3H), 7.14 (d, J=8.0 Hz, 1H), 4.64-4.67 (m, 1H), 3.52-3.80 (m, 1H), 3.51-3.52 (m, 1H), 3.34-3.44 (m, 4H), 2.70 (s, 3H), 2.43 (s, 3H), 1.71 (s, 3H).

(248) ##STR00144##

Example 22

(249) ##STR00145##

(250) Synthesis of Compound 22-2

(251) ##STR00146##

(252) To a solution of compound 22-1 (1.00 g, 5.12 mmol, 1.00 eq) and NBS (1.00 g, 5.63 mmol, 1.10 eq) in carbon tetrachloride (10.00 mL) was added benzoyl peroxide (124.02 mg, 512.00 μmol, 0.10 eq). The mixture was reacted under being stirred at 85° C. for 4 hours. The reaction mixture was directly concentrated under reduced pressure. The crude product was purified with a flash chromatography column to give compound 22-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.85 (d, J=2.4 Hz, 1H), 8.34-8.37 (m, 1H), 7.71 (d, J=8.8 Hz, 1H), 5.02 (s, 2H), 4.03 (s, 3H).

(253) Synthesis of Compound 22-3

(254) ##STR00147##

(255) At 0° C. and under the protection of nitrogen gas, thioacetic acid (288.90 mg, 3.80 mmol, 270.00 μL, 1.04 eq) slowly added dropwisely to a solution of compound 22-2 (1.00 g, 3.65 mmol, 1.00 eq) and potassium carbonate (948.40 mg, 6.86 mmol, 1.88 eq) in acetone (4.00 mL). After the completion of the dropwise addition, the mixture was stirred at 0° C. under the protection of nitrogen gas for 30 minutes, and then warmed up to 15° C. and stirred for 3 hours. The reaction mixture was concentrated to remove acetone. The resulting mixture was diluted with water (10 mL) and extracted with ethyl acetate (3×10 mL). The above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product purified with a thin-layer chromatography plate to give compound 22-3. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.83 (s, 1H), 8.31-8.32 (m, 1H), 7.77 (m, J=8.4 Hz, 1H), 4.56 (s, 2H), 4.00 (s, 3H), 2.34 (s, 3H).

(256) Synthesis of Compound 22-4

(257) ##STR00148##

(258) Compound 22-3 (800.00 mg, 2.97 mmol, 1.00 eq) was added to concentrated hydrochloric acid (5.88 g, 47.76 mmol, 4.00 mL, 16.08 eq), and the mixture was stirred at 90° C. for 2 hours. The reaction mixture was cooled to room temperature, and adjusted with a saturated sodium bicarbonate solution to pH=7. The mixture was extracted with ethyl acetate (3×10 mL) and separated into two phases. The above organic phases were combined. The organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a flash chromatography column to give compound 22-4. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.59 (m, J=2.0 Hz, 1H), 8.43 (dd, J=8.4, 2.0 Hz, 1H), 7.66 (d, J=8.4 Hz, 1H), 4.53 (s, 2H).

(259) Synthesis of Compound 22-5

(260) ##STR00149##

(261) Wet Pd/C (50.00 mg, 10% Pd) was added to a solution of compound 22-4 (100.00 mg, 512.30 μmol, 1.00 eq) in methanol (3.00 mL). The atmosphere was replaced with hydrogen gas three times. The mixture was stirred at 15° C. at a hydrogen balloon (15 psi) condition for 18 hours. The reaction mixture was filtered with diatomaceous earth. The filtrate was directly concentrated under reduced pressure. The above crude product was purified with a thin-layer chromatography plate to give compound 22-5. LCMS (ESI) m/z: 165.8 (M+1).

(262) Synthesis of Compound 22

(263) ##STR00150##

(264) Compound 1-11 (30 mg, 74.84 μmol, 1.00 eq) and compound 22-5 (99.218% purity) were successively added to a solution of HATU (36 mg, 94.68 μmol, 1.27 eq) in anhydrous N,N-dimethyl formamide (2 mL), and then triethylamine (21.90 mg, 216.42 μmol, 30 μL, 2.89 eq) was slowly added dropwisely. The mixture was stirred at 15° C. under the protection of nitrogen gas for 18 hours. Water (3 mL) was added to the reaction mixture to quench the reaction. The mixture was separated into two phases. The aqueous phase was extracted with methylene chloride (3×5 mL). The above organic phases were combined. The organic phase was washed with a saturated sodium bicarbonate solution (5 mL) and a saturated saline solution (5 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a preparative chromatography to give compound 22. LCMS (ESI) m/z: 548.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 10.08 (s, 1H), 8.01 (s, 1H), 7.66 (d, J=8.0 Hz, 1H), 7.29-7.46 (m, 5H), 4.80-4.83 (m, 1H), 4.30 (s, 2H), 3.97-4.03 (m, 1H), 3.58-3.63 (m, 1H), 2.78 (s, 3H), 2.45 (s, 3H), 1.71 (s, 3H).

(265) ##STR00151## ##STR00152##

Examples 23 and 24

(266) ##STR00153##

(267) Synthesis of Compound 23-1

(268) ##STR00154##

(269) Compound 21-1 (200.00 mg, 1.13 mmol, 1.00 eq) and sodium borohydride (85.50 mg, 2.26 mmol, 2.00 eq) were dissolved in absolute methanol (5.00 mL), and the mixture was stirred at 15° C. in a nitrogen atmosphere for 1 hour. The reaction mixture was directly concentrated under reduced pressure, and water (20 mL) was added thereto. Then the mixture was extracted with ethyl acetate (10 mL×2). The organic phase was washed with a saturated saline solution (30 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give compound 23-1, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.00-8.03 (m, 2H), 7.31 (d, J=8.0 Hz, 1H), 4.75 (br s, 1H), 3.18-3.25 (m, 2H), 2.92-2.97 (m, 2H), 1.61 (br s, 1H).

(270) Synthesis of Compound 23-2

(271) ##STR00155##

(272) Compound 23-1 (190 mg, 1.06 mmol, 1.00 eq) and diethylaminosulfur trifluoride (188.02 mg, 1.17 mmol, 154.12 μL, 1.1 eq) were dissolved in anhydrous methylene chloride (5.00 mL). The mixture was stirred at 15° C. in a nitrogen atmosphere for 16 hours. To the reaction mixture was added water (20 mL). Then, the mixture was extracted with methylene chloride (20 mL×2). The organic phase was washed with a saturated saline solution (30 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a thin-layer chromatography plate to give compound 23-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.03-8.07 (m, 2H), 7.31-7.35 (m, 1H), 5.27-5.61 (m, 1H), 3.26-3.28 (m, 2H), 3.19-3.20 (m, 2H).

(273) Synthesis of Compound 23-3

(274) ##STR00156##

(275) Compound 23-2 (30 mg, 165.60 μmol, 1.00 eq) and Pd/C (30 mg, 10% purity) were added to methanol (3 mL). The mixture was stirred at 15° C. under a hydrogen balloon atmosphere for 2 hours. The reaction mixture was directly filtered and concentrated under reduced pressure to give compound 23-3, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 6.90-6.95 (m, 1H), 6.52 (s, 1H), 6.41-6.49 (m, 1H), 5.24-5.50 (m, 1H), 2.95-3.11 (m, 4H).

(276) Synthesis of Compound 23-4

(277) ##STR00157##

(278) Compound 1-11 (60 mg, 149.67 μmol, 1.00 eq) and compound 23-3 (25 mg, 165.37 μmol, 1.10 eq) were added to anhydrous methylene chloride (3 mL), then HATU (56.91 mg, 149.67 μmol, 1.00 eq) was added, and diisopropylethylamine (58.03 mg, 449.01 μmol, 78.21 μL, 3.00 eq) was added dropwisely. The atmosphere was replaced with nitrogen gas three times. The mixture was stirred at 15° C. in a nitrogen atmosphere for 1 hour. The reaction mixture was directly concentrated under reduced pressure, then shaking washed with water (10 mL), and then extracted with ethyl acetate (10 mL×2). The organic phases were washed with a saturated saline solution (15 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a preparative chromatography to give compound 23-4. LCMS (ESI) m/z: 534.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δ ppm 8.66 (br s, 1H), 7.60 (br s, 1H), 7.43 (d, J=8.4 Hz, 2H), 7.30-7.37 (m, 3H), 7.20 (d, J=8.4 Hz, 1H), 5.33-5.64 (m, 1H), 4.61-4.65 (m, 1H), 3.79-3.80 (m, 1H), 3.46-3.51 (m, 1H), 3.06-3.30 (m, 4H), 2.70 (s, 3H), 2.42 (s, 3H), 1.70 (s, 3H).

(279) Synthesis of Compounds 23 and 24

(280) ##STR00158##

(281) Compound 23-4 (28 mg, 52.43 μmol) was subjected to the SFC separation ((chromatography column: OJ (250 mm×30 mm, 5 μm); mobile phase: [0.1% NH.sub.3H.sub.2O EtOH]; B %: 30%-30%, 60 mL/min)) to give compound 23 (Rt=5.404 min). LCMS (ESI) m/z: 534.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.99 (br s, 1H), 7.50 (s, 1H), 7.23-7.34 (m, 5H), 7.06 (br d, J=8.0 Hz, 1H), 5.36 (d, J=53.2 Hz 1H), 4.58-4.61 (m, 1H), 3.71-3.75 (m, 1H), 3.43-3.47 (m, 1H), 2.78-3.20 (m, 4H), 2.60 (s, 3H), 2.33 (s, 3H), 1.60 (s, 3H).

(282) Compound 24 (Rt=5.702 min). LCMS (ESI) m/z: 534.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.00 (s, 1H), 7.52 (s, 1H), 7.20-7.34 (m, 5H), 7.07 (br d, J=8.0 Hz, 1H), 5.36 (d, J=52.0 Hz, 1H), 4.58-4.61 (bm, 1H), 3.65-3.72 (m, 1H), 3.45-3.49 (m, 1H), 3.02-3.31 (m, 4H), 2.60 (s, 3H), 2.33 (s, 3H), 1.60 (s, 3H).

(283) ##STR00159##

Example 25

(284) ##STR00160##

(285) Synthesis of Compound 25-1

(286) ##STR00161##

(287) At 15° C. and in a nitrogen atmosphere, to a mixed liquor of compound 16-1 (300 mg, 2.22 mmol, 1 eq), sodium bicarbonate (279.70 mg, 3.33 mmol, 129.49 μL, 1.5 eq) in methylene chloride (10 mL) and methanol (2.5 mL) was added dropwisely iodine chloride (1 M, 2.44 mL, 1.1 eq), and then the mixture was stirred at the same temperature for 4 hours. At 15° C. and under being stirred, to the reaction mixture was added dropwisely a saturated Na.sub.2SO.sub.3 solution (25 mL), and then the mixture was extracted with methylene chloride (10 mL×2). The organic phase was washed with a saturated saline solution (20 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a flash chromatography column to give compound 25-1. LCMS (ESI) m/z: 261.8 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 6.88 (d, J=8.0 Hz, 1H), 6.57 (d, J=8.0 Hz, 1H), 5.12 (s, 2H), 4.89 (s, 2H), 4.00 (br s, 2H).

(288) Synthesis of Compound 25-2

(289) ##STR00162##

(290) At 15° C., compound 25-1 (140 mg, 489.22 μmol, 1 eq), cesium fluoride (260.10 mg, 1.71 mmol, 63.13 μL, 3.5 eq), [1,1′-bis(diphenylphosphine)ferrocene]palladium (II) dichloride (35.80 mg, 48.92 μmol, 0.1 eq) and methylboronic acid (87.85 mg, 1.47 mmol, 3 eq) were dissolved in dioxane (5 mL), and the mixture was stirred at 80° C. in a nitrogen atmosphere for 4 hours. The reaction mixture was filtered, then shaking washed with water (20 mL), and then extracted with ethyl acetate (10 mL×2). The organic phases were combined, washed with a saturated saline solution (20 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a thin-layer chromatography plate to give compound 25-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 6.81 (d, J=8.0 Hz, 1H), 6.56 (d, J=8.00 Hz, 1H), 4.99 (s, 4H), 3.52 (br s, 2H), 1.98 (s, 3H).

(291) Synthesis of Compound 25

(292) ##STR00163##

(293) Compound 1-11 (50 mg, 124.73 μmol, 1.00 eq) and compound 25-2 (27.91 mg, 187.09 μmol, 1.5 eq) were dissolved in anhydrous methylene chloride (3 mL), HATU (47.42 mg, 124.73 μmol, 1.00 eq) was added, and diisopropylethylamine (48.36 mg, 374.18 μmol, 65.17 μL, 3.00 eq) was added dropwisely. The mixture was stirred at 15° C. and in a nitrogen atmosphere for 2 hours. The reaction mixture was shaking washed with water (10 mL). The organic phase was washed with a saturated saline solution (20 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified with a preparative chromatography to give compound 25. LCMS (ESI) m/z: 532.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.44 (s, 1H), 7.57 (d, J=7.6 Hz, 1H), 7.35 (d, J=8.4 Hz, 2H), 7.26 (d, J=8.4 Hz, 2H), 6.96 (d, J=8.0 Hz, 1H), 4.99-5.02 (m, 4H), 4.55-4.59 (m, 1H), 3.70-3.75 (m, 1H), 3.45-3.50 (m, 1H), 2.61 (s, 3H), 2.34 (s, 3H), 2.08 (s, 3H), 1.61 (s, 3H).

(294) ##STR00164##

Example 26

(295) ##STR00165##

(296) Example 26 was synthesized with reference to Example 25.

(297) LCMS (ESI) m/z: 532.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.39 (s, 1H), 7.75 (s, 1H), 7.36 (d, J=8.8 Hz, 2H), 7.27 (d, J=8.8 Hz, 2H), 6.97 (s, 1H), 4.98 (d, J=9.6 Hz, 4H), 4.53-4.57 (m, 1H), 3.73-3.78 (m, 1H), 3.42-3.47 (m, 1H), 2.62 (s, 3H), 2.34 (s, 3H), 2.24 (s, 3H), 1.62 (s, 3H).

(298) ##STR00166## ##STR00167##

Example 27

(299) ##STR00168##

(300) Synthesis of Compound 27-2

(301) ##STR00169##

(302) A mixture of ortho-xylene (20.00 g, 188.39 mmol, 22.73 mL, 1.00 eq), NBS (70.41 g, 395.62 mmol, 2.10 eq), benzoyl peroxide (912.70 mg, 3.77 mmol, 0.02 eq) and chloroform (200.00 mL) was stirred at 80° C. for 5 hours. The reaction mixture was cooled to room temperature, then diluted with methylene chloride (200 mL), and washed with water (100 mL×2) and washed with a saturated saline solution (100 mL) respectively. The organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The obtained solid was recrystallized at 80° C. with (petroleum ether/ethanol=30:1; 240 mL/8 mL) and cooled to room temperature. A solid precipitated and was filtered. The filter cake washed with petroleum ether (50 mL). The filter cake was oven-dried to give compound 27-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.41-7.35 (m, 2H), 7.35-7.29 (m, 2H), 4.68 (s, 4H).

(303) Synthesis of Compound 27-3

(304) ##STR00170##

(305) At 0° C., to a solution of compound 27-2 (5 g, 18.94 mmol, 2.55 mL, 1 eq) and concentrated sulphuric acid (30 mL) was added in batch potassium nitrate (2.30 g, 22.73 mmol, 1.2 eq). Then the mixture was stirred at 0° C. for 3 hours. The reaction mixture became red-brown. The reaction mixture was slowly added dropwisely to a 500 mL beaker containing ice cubes (200 g), and a light yellow solid precipitated. Then the mixture was stirred at 0° C. for 1 hour and then filtered. The filter cake was washed with water (100 mL). The filter cake was oven-dried to give compound 27-3, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δppm 8.39 (d, J=2.5 Hz, 1H), 8.19 (dd, J=2.4, 8.4 Hz, 1H), 7.78 (d, J=8.3 Hz, 1H), 4.94 (s, 2H), 4.90 (s, 2H).

(306) Synthesis of Compound 27-4

(307) ##STR00171##

(308) Sodium metal (892.94 mg, 38.84 mmol, 920.56 μL, 2.4 eq) was added to ethanol (20 mL). At 10° C., the mixture was stirred for 0.5 hour until Na completely disappeared. Then a solution of diethyl malonate (3.00 g, 18.73 mmol, 2.83 mL, 1.16 eq) in tetrahydrofuran (10 mL) was added, and a mixed liquor of compound 27-3 (5 g, 16.18 mmol, 1 eq) and tetrahydrofuran (10 mL) was quickly added. Then, the resulting mixture was refluxed at 80° C. under the protection of nitrogen gas for 1 hour. The reaction mixture was concentrated under reduced pressure. Water (60 mL) was added. Then the mixture was extracted with ethyl acetate (50 mL×3). The combined organic phases were washed with a saturated saline solution (50 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure, separated and purified with column chromatography to give compound 27-4. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δppm 8.13 (s, 1H), 8.06 (dd, J=2.3, 8.3 Hz, 1H), 7.51 (d, J=8.3 Hz, 1H), 4.18-4.13 (m, 4H), 3.59 (s, 4H), 1.19-1.16 (m, 6H).

(309) Synthesis of Compound 27-5

(310) ##STR00172##

(311) Compound 27-4 (1.3 g, 4.23 mmol, 1 eq) was dissolved in ethanol (20 mL), and then stannous chloride dihydrate (4.77 g, 21.15 mmol, 1.76 mL, 5 eq) was added. The mixture was stirred at 80° C. for 3 hours. The reaction mixture was concentrated under reduced pressure, then adjusted with NaOH solution (4 N) to pH=10, and then extracted with ethyl acetate (40 mL×3). The combined organic phases were washed with a saturated saline solution (50 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure, separated and purified with column chromatography to give compound 27-5. LCMS (ESI) m/z: 277.9 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 6.97 (d, J=8.0 Hz, 1H), 6.56-6.48 (m, 2H), 4.20 (q, J=7.2 Hz, 4H), 3.62 (br s, 2H), 3.50 (s, 2H), 3.48 (s, 2H), 1.27-1.24 (t, J=7.2 Hz, 6H).

(312) Synthesis of Compound 27-6

(313) ##STR00173##

(314) Compound 27-5 (500 mg, 1.80 mmol, 1 eq) was dissolved in tetrahydrofuran (10 mL) at 0° C., and then lithium aluminum hydride (157.39 mg, 4.15 mmol, 2.3 eq) was added. The mixture was stirred at 0° C. for 1 hour. Water (0.2 mL) was added to quench the reaction. Then the mixture was concentrated under reduced pressure, then slurried with ethyl acetate (100 mL) for 10 minutes at 10° C., and filtered. The filter cake was washed with ethyl acetate (50 mL). The filtrate was concentrated under reduced pressure to give compound 27-6, which was directly used in the next step without any further purification.

(315) Synthesis of Compound 27-7

(316) ##STR00174##

(317) Compound 27-6 (150 mg, 776.23 μmol, 1 eq) was dissolved in tetrahydrofuran (3 mL). Sodium hydride (46.57 mg, 1.16 mmol, 60% purity, 1.5 eq) was added at 0° C. The mixture was stirred 40 minutes. Then a mixed liquor of para-toluenesulfonyl chloride (147.99 mg, 776.23 μmol, 1 eq) and tetrahydrofuran (3 mL) was added. The mixture was stirred at 0° C. for 40 minutes and then concentrated under reduced pressure to give an oily substance. To the oily substance was added water (50 mL). The mixture was extracted with ethyl acetate (50 mL×3). The combined organic phases were washed with a saturated saline solution (50 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure, and purified with a thin-layer chromatography plate to give compound 27-7. LCMS (ESI) m/z: 348.1 (M+1).

(318) Synthesis of Compound 27-8

(319) ##STR00175##

(320) Compound 27-7 (50 mg, 143.91 μmol, 1 eq) was dissolved in tetrahydrofuran (3 mL), and then sodium hydride (57.56 mg, 1.44 mmol, 60% purity, 10 eq) was added. The mixture was stirred at 70° C. for 5 hours. Water (0.5 mL) was added to quench the reaction. The reaction mixture was concentrated under reduced pressure to give an oily substance. To the oily substance was added water (30 mL). The mixture was extracted with (methylene chloride/methanol=10/1) (40 mL×3). The combined organic phases were washed with a saturated saline solution (50 mL), then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure, and purified with a thin-layer chromatography plate to give compound 27-8. LCMS (ESI) m/z: 175.9 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 6.98 (d, J=8.0 Hz, 1H), 6.57 (s, 1H), 6.51 (dd, J=2.1, 7.9 Hz, 1H), 4.67 (s, 4H), 3.62 (br s, 2H), 3.16 (s, 2H), 3.14 (s, 2H).

(321) Synthesis of Compound 27

(322) ##STR00176##

(323) Diisopropylethylamine (58.03 mg, 449.01 μmol, 78.21 μL, 3 eq) was added to a solution of compound 1-11 (0.06 g, 149.67 μmol, 1 eq), compound 27-8 (15.74 mg, 89.80 μmol, 388.88 μL, 0.6 eq) and HATU (62.60 mg, 164.64 μmol, 1.1 eq) in anhydrous methylene chloride (10 mL). The mixture was reacted under being stirred at 15° C. under the protection of nitrogen gas for 1 hour. The reaction mixture was diluted with methylene chloride (10 mL), then successively washed with 1N hydrochloric acid (5 mL), water (5 mL) and a saturated saline solution (10 mL), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure. The crude product was purified with a preparative chromatography to give compound 27. LCMS (ESI) m/z: 558.1 (M+1). .sup.1HNMR (CDCl.sub.3 400 MHz) δppm 8.58 (s, 1H), 7.52 (s, 1H), 7.42 (d, J=8.8 Hz, 2H), 7.33 (d, J=8.8 Hz, 2H), 7.23-7.26 (m, 1H), 7.11-7.23 (m, 1H), 4.66 (s, 4H), 4.58-4.65 (m, 1H), 3.74-3.79 (m, 1H), 3.43-3.48 (m, 1H), 3.21 (s, 2H), 3.18 (s, 2H), 2.67 (s, 3H), 2.40 (s, 3H), 1.69 (s, 3H).

(324) ##STR00177##

Example 28

(325) ##STR00178##

(326) Synthesis of Compound 28-2

(327) ##STR00179##

(328) At 0° C. and under the protection of nitrogen gas, a solution of compound 28-1 (6 g, 32.87 mmol, 1 eq) in tetrahydrofuran (10 mL) was slowly added dropwisely to a suspension of lithium aluminum hydride (2.50 g, 65.76 mmol, 2 eq) and zinc chloride (2.69 g, 19.72 mmol, 923.71 μL, 0.6 eq) in tetrahydrofuran (20 mL). After the completion of the dropwise addition, the mixture was warmed up to 10° C. and stirred for 6 hours. The reaction mixture was cooled to 0° C. Water (10 mL) was added to quench the reaction (a white precipitate occurred in the quenching process). The mixture was adjusted with an aqueous hydrochloric acid solution (2M) to pH=about 6. The above-mixed solution was extracted with ethyl acetate (3×20 mL) and separated into two phases. The organic phases were combined, then dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was added to ethyl acetate (3 mL) and petroleum ether (10 mL). The mixture was heated to 75° C. and stirred under reflux for 30 minutes, and cooled to room temperature. Then petroleum ether (20 mL) was added, and the mixture was stirred for 20 minutes (a white solid precipitated) and filtered. The filter cake was directly oven-dried to give compound 28-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.30 (s, 1H), 7.21-7.22 (m, 2H), 4.63 (s, 4H), 2.76 (br s, 1H), 2.67 (br s, 1H).

(329) Synthesis of Compound 28-3

(330) ##STR00180##

(331) At 0° C. and under the protection of nitrogen gas, phosphorus tribromide (4.70 g, 17.38 mmol, 1.2 eq) was slowly added dropwisely to a solution of compound 28-2 (2.5 g, 14.48 mmol, 1 eq) in methylene chloride (20 mL). After the completion of the dropwise addition, the mixture was warmed up to 10° C., stirred for 5 hours, diluted, and extracted with methylene chloride (3×20 mL). The above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a flash chromatography column to give compound 28-3. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.30 (d, J=2.4 Hz, 1H), 7.21-7.24 (m, 2H), 4.53 (d, J=8.8 Hz, 4H).

(332) Synthesis of Compound 28-4

(333) ##STR00181##

(334) Neutral alumina (30 g, 294.23 mmol, 35.12 eq) was added to a solution of compound 28-3 (2.5 g, 8.38 mmol, 1 eq) in n-hexane (40 mL). The mixture was stirred at 75° C. for 3 hours. The reaction mixture was cooled to 10° C. and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a flash chromatography column to give compound 28-4. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.22-7.25 (m, 2H), 7.15-7.17 (m, 1H), 5.08 (s, 4H).

(335) Synthesis of Compound 28-5

(336) ##STR00182##

(337) At −10° C., a solution of compound 28-4 (300 mg, 1.94 mmol, 1 eq) in concentrated sulphuric acid (2 mL) was slowly added dropwisely to a solution of potassium nitrate (195.00 mg, 1.93 mmol, 9.94e-1 eq) in concentrated sulphuric acid (6 mL). After the completion of the dropwise addition, the mixture was stirred at −10° C. for 20 minutes. The reaction mixture was poured into ice (about 10 mL). The mixture was stirred for 10 minutes, and extracted with ethyl acetate (3×10 mL). The above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a flash chromatography column to give compound 28-5. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.78 (s, 1H), 7.44 (s, 1H), 5.15 (s, 4H).

(338) Synthesis of Compound 28-6

(339) ##STR00183##

(340) Stannous chloride dihydrate (900 mg, 3.99 mmol, 332.10 μL, 3.98 eq) was added to a solution of compound 28-5 (200 mg, 1.00 mmol, 1.00 eq) in methanol (4 mL). The mixture was stirred at 20° C. for 5 hours. The reaction mixture was directly concentrated under reduced pressure. The above crude product was purified with a thin-layer chromatography plate to give compound 28-6. .sup.1H NMR (400 MHz, CD.sub.3OD) δppm 7.33 (s, 1H), 7.0-7.06 (m, 1H), 5.01 (m, 4H).

(341) Synthesis of Compound 28

(342) ##STR00184##

(343) At 0° C., POCl.sub.3 (133.87 mg, 873.08 μmol, 81.13 μL, 5 eq) was slowly added dropwisely to a solution of compound 28-6 (40 mg, 235.84 μmol, 1.35 eq) and compound 1-11 (70 mg, 174.62 μmol, 1.00 eq) in pyridine (3 mL). At 0° C. and under the protection of nitrogen gas, the mixture was stirred for 1 hour. Ice water (3 mL) was added to the reaction mixture to quench the reaction. The above-mixed solution was adjusted with a dilute aqueous hydrochloric acid solution (0.5M) to pH=about 6 and extracted with ethyl acetate (3×5 mL). The above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a preparative chromatography to give compound 28. LCMS (ESI) m/z: 552.0 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.71 (s, 1H), 8.22 (s, 1H), 7.36 (d, J=8.4 Hz, 2H), 7.26 (d, J=8.4 Hz, 2H), 7.17 (s, 1H), 4.98 (d, J=4.0 Hz, 4H), 4.58 (t, J=6.8 Hz, 1H), 3.66-3.72 (m, 1H), 3.51-3.57 (m, 1H), 2.62 (s, 3H), 2.34 (s, 3H), 1.62 (s, 3H).

(344) ##STR00185##

Example 29

(345) ##STR00186##

(346) Synthesis of Compound 29-2

(347) ##STR00187##

(348) At 0° C. and under the protection of nitrogen gas, a solution of compound 29-1 (5 g, 30.10 mmol, 1 eq) in anhydrous tetrahydrofuran (40 mL) was slowly added to a suspension of lithium aluminum hydride (2.28 g, 60.20 mmol, 2 eq) and zinc chloride (2.46 g, 18.06 mmol, 845.91 μL, 0.6 eq) in anhydrous tetrahydrofuran (100 mL). After the addition, the mixture was reacted at 10° C. for 16 hours. Water (3 mL) was slowly added to the reaction mixture to quench the reaction, and then water (50 mL) was added to the reaction mixture. The aqueous phase was extracted with ethyl acetate (50 mL×2). The organic phases were combined, dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to give compound 29-2, which was directly used in the next step without purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.21-7.23 (m, 1H), 6.93-7.03 (m, 2H), 4.53-4.55 (m, 4H).

(349) Synthesis of Compound 29-3

(350) ##STR00188##

(351) At 0° C. and under the protection of nitrogen gas, phosphorus tribromide (7.76 g, 28.66 mmol, 1.2 eq) was slowly added to a solution of compound 29-2 (3.73 g, 23.89 mmol, 1 eq) in anhydrous methylene chloride (100 mL). After the addition, the mixture was reacted at 10° C. under the protection of nitrogen gas for 6 hours. The reaction mixture was washed with water (50 mL). The organic phase was dried over anhydrous sodium sulfate and filtered and concentrated under reduced pressure. The crude product was purified with a flash chromatography column to give compound 29-3.

(352) Synthesis of Compound 29-4

(353) ##STR00189##

(354) Neutral alumina (40 g, 392.31 mmol, 29.89 eq) was added to a solution of compound 29-3 (3.7 g, 13.12 mmol, 1 eq) in n-hexane (80 mL). After the addition, the mixture was reacted at 75° C. for 20 hours. The reaction mixture was cooled to 50° C., and the insoluble substance was filtered off while hot. Then the filter cake was washed with methylene chloride (50 mL). The filtrates were combined and evaporated to dryness under reduced pressure to give compound 29-4, which was directly used in the next step without purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.07-7.09 (m, 1H), 6.84-6.90 (m, 2H), 5.00 (s, 4H).

(355) Synthesis of Compound 29-5

(356) ##STR00190##

(357) Potassium nitrate (878.27 mg, 8.69 mmol, 1 eq) was added to a solution of concentrated sulphuric acid (10 mL). Compound 29-4 (1.2 g, 8.69 mmol, 1 eq) was dissolved in concentrated sulphuric acid (5 mL) that was cooled down at −10° C. in an iced salt bath, and then added to the above reaction mixture in an iced salt bath. After the addition, the mixture was reacted at −10° C. in an iced salt bath for 30 minutes. The reaction mixture was slowly added dropwisely to the continuously stirred crushed ice (100 mL). The mixture was filtered to give a light brown solid as a crude product. The crude product was dissolved in ethyl acetate (50 mL). Then the mixture was dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to give compound 29-5, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.71 (d, J=1.6 Hz, 1H), 8.51 (dd, J=2.0, 8.4 Hz, 1H), 7.65 (d, J=8.4 Hz, 1H), 5.38 (s, 4H).

(358) Synthesis of Compound 29-6

(359) ##STR00191##

(360) Compound 29-5 (0.183 g, 999.25 μmol, 1 eq) and stannous chloride dihydrate (901.91 mg, 4.00 mmol, 332.81 μL, 4 eq) were added to absolute methanol (5 mL). After the addition, the mixture was reacted at 30° C. for 4 hours. The reaction mixture was directly concentrated under reduced pressure. The residue was dissolved in ethyl acetate (50 mL). The insoluble substance was filtered off. The filtrate was dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to give compound 29-6, which was directly used in the next step without any further purification. LCMS (ESI) m/z: 153.9 (M+1).

(361) Synthesis of Compound 29

(362) ##STR00192##

(363) At 0° C. and under the protection of nitrogen gas, POCl.sub.3 (71.27 mg, 464.83 μmol, 43.20 μL, 2 eq) was added to a solution of compound 1-11 (100 mg, 232.41 μmol, 1 eq) and compound 29-6 (42.71 mg, 278.90 μmol, 1.2 eq) in pyridine (2 mL). After the addition, the mixture was reacted at 15° C. for 2 hours. Water (5 mL) was added to the reaction mixture. The aqueous phase was adjusted with hydrochloric acid to pH=7 and extracted with methylene chloride (5 mL×3). The organic phases were combined, dried over anhydrous sodium sulfate, filtered, then concentrated under reduced pressure, and purified with a thin-layer chromatography plate to give compound 29. LCMS (ESI) m/z: 536.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.80 (br s, 1H), 8.13 (d, J=6.8 Hz, 1H), 7.36 (d, J=8.4 Hz, 2H), 7.26 (d, J=8.8 Hz, 2H), 6.88 (d, J=10.0 Hz, 1H), 4.97 (s, 4H), 4.5-4.57 (m, 1H), 3.64-3.66 (m, 1H), 3.52-3.54 (m, 1H), 2.61 (s, 3H), 2.34 (s, 3H), 1.62 (s, 3H).

(364) ##STR00193##

Example 30

(365) ##STR00194##

(366) Synthesis of Compound 30-2

(367) ##STR00195##

(368) Compound 30-1 (0.5 g, 2.79 mmol, 1 eq) and Pd/C (0.5 g, 10% purity) were added to methanol (5 mL). After the addition, the atmosphere was replaced with a balloon filled with hydrogen gas for 3 times. Then the mixture was reacted at 20° C. under the protection of hydrogen balloon (15 psi) for 12 hours. The insoluble substance was filtered off. The filtrate was concentrated under reduced pressure. The residue was dissolved in methylene chloride (50 mL). The mixture was filtered to obtain a filtrate. Then the filtrate was concentrated under reduced pressure to give compound 30-2, which was directly used in the next step without any further treatment. .sup.1H NMR (400 MHz, CD.sub.3OD) δppm 7.19 (d, J=9.2 Hz, 1H), 6.96-6.98 (m, 2H), 5.12 (s, 3H).

(369) Synthesis of Compound 30

(370) ##STR00196##

(371) Compound 1-11 (0.1 g, 232.41 μmol, 1 eq), compound 30-2 (52.00 mg, 348.62 μmol, 1.5 eq) and HATU (106.04 mg, 278.90 μmol, 1.2 eq) were added to anhydrous N,N-dimethyl formamide (2 mL). Then diisopropylethylamine (60.08 mg, 464.83 μmol, 80.96 μL, 2 eq) was added to the above solution at 20° C. After the addition, the mixture was reacted at 20° C. for 12 hours. Water (5 mL) was added to the reaction mixture. The resulting mixture was extracted with methylene chloride (5 mL×3). The organic phase was concentrated under reduced pressure. Then water (10 mL) was added. The mixture was lyophilized to remove the residual N,N-dimethyl formamide, and purified with a preparative chromatography to give compound 30. LCMS (ESI) m/z: 532.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.65 (br s, 1H), 8.09 (d, J=6.8 Hz, 1H), 7.68 (d, J=8.0 Hz, 1H), 7.33-7.35 (m, 2H), 7.24-7.27 (m, 3H), 5.15 (s, 2H), 4.62-4.66 (m, 1H), 3.80-3.86 (m, 1H), 3.46-3.51 (m, 1H), 2.64 (s, 3H), 2.35 (s, 3H), 1.63 (s, 3H).

(372) ##STR00197##

Example 31

(373) ##STR00198##

(374) Synthesis of Compound 31-2

(375) ##STR00199##

(376) At 20° C. and under the protection of nitrogen gas, to a constantly stirred solution of compound 31-1 (0.5 g, 4.54 mmol, 757.58 μL, 1 eq) dissolved in n-butyl alcohol (10 mL) was added dropwisely a solution of lithium bromide monohydrate (1.19 g, 11.35 mmol, 2.5 eq) and lithium hydroxide monohydrate (400.16 mg, 9.53 mmol, 2.1 eq) dissolved in water (1 mL). After the completion of the dropwise addition, the reaction mixture was warmed up to 110° C. and stirred for 0.1 hours. Then 1,2-di(2-chloroethoxy)ethane (849.27 mg, 4.54 mmol, 532.29 μL, 1 eq) was added. The stirring at the same temperature was kept on for 5 hours. The reaction mixture was adjusted with concentrated HCl to pH=4, and then directly concentrated under reduced pressure. To the residue were added methylene chloride (50 mL) and water (30 mL). The mixture was stirred for 0.5 hour and separated into two phases. The organic phase was washed with a saturated saline solution (30 mL), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure. The compound as a crude product was purified with a flash chromatography column to give compound 31-2. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 6.89-6.90 (m, 4H), 4.09-4.11 (m, 4H), 3.73-3.79 (m, 8H).

(377) Synthesis of Compound 31-3

(378) ##STR00200##

(379) A solution of compound 31-2 (50 mg, 222.96 μmol, 1 eq) in acetonitrile (2 mL) was warmed up to 85° C. Concentrated nitric acid (38 mg, 337.71 μmol, 27.14 μL, 56% purity, 1.51 eq) was slowly added dropwisely. After the completion of the dropwise addition, the mixture was stirred at 85° C. for 0.5 hour. The reaction mixture was cooled to room temperature and poured into ice (about 10 mL) to quench the reaction. The mixture was extracted with ethyl acetate (3×10 mL). The above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 31-3, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.86-7.89 (m, 1H), 7.81 (d, J=2.8 Hz, 1H), 6.93 (d, J=8.8 Hz, 1H), 4.17-4.23 (m, 4H), 3.84-3.85 (m, 2H), 3.77-3.80 (m, 2H), 3.71 (s, 4H).

(380) Synthesis of Compound 31-4

(381) ##STR00201##

(382) Wet Pd/C (50 mg, 10% purity) was added to a solution of compound 31-3 (50 mg, 185.70 μmol, 1.00 eq) in methanol (10 mL). The atmosphere was replaced with hydrogen gas three times. The mixture was stirred at 15° C. at a hydrogen balloon (15 psi) condition for 2 hours. The reaction mixture was filtered with diatomaceous earth. The filtrate was directly concentrated under reduced pressure to give compound 31-4, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 6.75 (d, J=8.4 Hz, 1H), 6.26 (d, J=2.4 Hz, 1H), 6.18 (dd, J=8.4, 2.4 Hz, 1H), 3.98-4.05 (m, 4H), 3.75-3.84 (m, 2H), 3.71 (s, 6H), 3.27 (br s, 2H).

(383) Synthesis of Compound 31

(384) ##STR00202##

(385) Compound 1-11 (35 mg, 87.31 μmol, 1.00 eq) and compound 31-4 (25 mg, 104.49 μmol, 1.20 eq) were successively added to a solution of HATU (35.00 mg, 92.05 μmol, 1.05 eq) in anhydrous methylene chloride (2 mL), and then triethylamine (29.08 mg, 287.38 μmol, 40 μL, 3.29 eq) was slowly added dropwisely. The mixture was stirred at 10° C. under the protection of nitrogen gas for 6 hours. Water (3 mL) was added to the reaction mixture to quench the reaction. The mixture was extracted with methylene chloride (3×5 mL), and the above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a preparative chromatography to give compound 31. LCMS (ESI) m/z: 622.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.82 (m, 1H), 7.23-7.38 (m, 5H), 6.92-6.94 (m, 1H), 6.81-6.83 (m, 1H), 4.57-4.59 (m, 1H), 3.97-4.15 (m, 4H), 3.70-3.80 (m, 9H), 3.43-3.45 (m, 1H), 2.61 (s, 3H), 2.34 (s, 3H), 1.61 (s, 3H).

(386) ##STR00203##

Example 32

(387) ##STR00204##

(388) Synthesis of Compound 32-2

(389) ##STR00205##

(390) Compound 32-1 (0.78 g, 3.66 mmol, 1 eq), tert-butyl carbamate (643.39 mg, 5.49 mmol, 1.5 eq), tris(dibenzylideneacetone) dipalladium (335.29 mg, 366.15 μmol, 0.1 eq), cesium carbonate (2.39 g, 7.32 mmol, 2 eq) and 4,5-bis(diphenylphosphine)-9,9-dimethylxanthene (211.86 mg, 366.15 μmol, 0.1 eq) were added to 1,4-dioxane (10 mL). After the addition, the mixture was reacted at 100° C. under the protection of nitrogen gas for 12 hours. To the reaction mixture was added water (20 mL) and then ethyl acetate (20 mL). The insoluble substance was filtered off. Then the aqueous phase was extracted with ethyl acetate (10 mL). The organic phases were combined, dried over anhydrous sodium sulfate, filtered, then concentrated under reduced pressure, and purified with a flash chromatography column to give compound 32-2. LCMS (ESI) m/z: 250.1 (M+1).

(391) Synthesis of Compound 32-3

(392) ##STR00206##

(393) Trifluoroacetic acid (3.85 g, 33.77 mmol, 2.5 mL, 18.30 eq) was added to compound 32-2 (0.46 g, 1.85 mmol, 1 eq) in anhydrous methylene chloride (20 mL). After the addition, the mixture was reacted at 20° C. for 12 hours. The reaction mixture was washed with water (20 mL). The aqueous phase was adjusted with a saturated sodium bicarbonate solution to pH=7 and then extracted with methylene chloride (10 mL×2). The organic phases were combined, dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to give compound 32-3, which was directly used in the next step without any further purification. LCMS (ESI) m/z: 149.8 (M+1).

(394) Synthesis of Compound 32

(395) ##STR00207##

(396) At 0° C., POCl.sub.3 (76.50 mg, 498.90 μmol, 46.36 μL, 2 eq) was added to a solution of compound 1-11 (100 mg, 249.45 μmol, 1 eq) and compound 32-3 (44.65 mg, 299.34 μmol, 1.2 eq) in pyridine (2 mL). After the addition, the mixture was warmed up to 20° C. and reacted for 1.5 hours. Water (3 mL) was added to the reaction mixture to quench the reaction. Then the aqueous phase was adjusted with hydrochloric acid (2N) to pH=7. The aqueous phase was extracted with methylene chloride (5 mL×3). The organic phase was dried over anhydrous sodium sulfate, filtered, then concentrated under reduced pressure, and purified with a preparative thin layer chromatography plate to give compound 32. LCMS (ESI) m/z: 532.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.98 (br s, 1H), 7.94 (d, J=6.8 Hz, 1H), 7.67 (d, J=8.0 Hz, 1H), 7.32-7.36 (m, 3H), 7.24-7.27 (m, 2H), 5.08-5.16 (m, 2H), 4.57-4.61 (m, 1H), 3.78-3.84 (m, 1H), 3.45-3.50 (m, 1H), 2.63 (s, 3H), 2.36 (s, 3H), 1.63 (s, 3H).

(397) ##STR00208##

Example 33

(398) ##STR00209##

(399) Synthesis of Compound 33-2

(400) ##STR00210##

(401) Compound 33-1 (0.5 g, 2.51 mmol, 1 eq), CuO (19.99 mg, 251.25 μmol, 3.16 μL, 0.1 eq) and cuprous iodide (478.51 mg, 2.51 mmol, 1 eq) was added to concentrated ammonia water (5.46 g, 155.80 mmol, 6 mL, 62.01 eq). To the obtained solution was added dropwisely a few drops of N-methyl pyrrolidone. In a microwave synthesis instrument, the reaction was carried out at 140° C. for 0.5 hour and then at 150° C. for 1 hour. To the reaction mixture was added water (10 mL) to dilute the reaction mixture, and then methylene chloride (10 mL) was added. The resulting mixture was filtered to remove the insoluble substance. The aqueous phase was extracted with methylene chloride (10 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to give compound 33-2, which was directly used in the next step without any further purification. LCMS (ESI) m/z: 136.0 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.55 (d, J=9.6 Hz, 1H), 7.67 (dd, J=2.0, 9.6 Hz, 1H), 6.50 (s, 1H), 4.63 (br s, 2H).

(402) Synthesis of Compound 33

(403) ##STR00211##

(404) At 0° C. and under the protection of nitrogen gas, POCl.sub.3 (35.64 mg, 232.41 μmol, 21.60 μL, 2 eq) was added to a solution of compound 1-11 (50 mg, 116.21 μmol, 1 eq) and compound 33-2 (23.55 mg, 174.31 μmol, 1.5 eq) in pyridine (1 mL). After the addition, the mixture was reacted at 20° C. for 2 hours. Water (2 mL) was added to the reaction mixture to quench the reaction. Then the aqueous phase was adjusted with 2N HCl to pH=7. The aqueous phase was extracted with methylene chloride (5 mL×3). The organic phase was dried over anhydrous sodium sulfate, filtered, then concentrated under reduced pressure, purified with a preparative chromatography to give compound 33. LCMS (ESI) m/z: 518.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.87 (br s, 1H), 8.33 (s, 1H), 7.60 (d, J=9.2 Hz, 1H), 7.37 (d, J=8.8 Hz, 2H), 7.27 (d, J=8.8 Hz, 2H), 7.16-7.17 (m, 1H), 4.55-4.58 (m, 1H), 3.76-3.82 (m, 1H), 3.44-3.48 (m, 1H), 2.64 (s, 3H), 2.36 (s, 3H), 1.64 (s, 3H).

(405) ##STR00212## ##STR00213##

Examples 34 and 35

(406) ##STR00214##

(407) Synthesis of Compound 34-2

(408) ##STR00215##

(409) 3-chloroperbenzoic acid (3.09 g, 15.23 mmol, 85% purity) was added to a solution of compound 34-1 (4.45 g, 17.89 mmol, 1 eq) and p-toluenesulfonic acid monohydrate (3.43 g, 18.01 mmol, 1.01 eq) in acetonitrile (25 mL). The resulting mixture was warmed up to 77° C. and stirred for 30 minutes. Then 1,3,5-trimethylbenzene (2.16 g, 17.99 mmol, 2.5 mL, 1.01 eq) was slowly added dropwisely. After the completion of the dropwise addition, the resulting mixture was stirred at 77° C. for 3 hours. The reaction mixture was cooled to room temperature, and concentrated under reduced pressure. The residue was washed with petroleum ether (20 mL) and filtered. The filter cake was oven-dried to give compound 34-2, which was directly used in the next step without any further purification. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δppm 8.24 (d, J=9.2 Hz, 1H), 8.17 (d, J=8.8 Hz, 1H), 8.06 (d, J=8.8 Hz, 2H), 7.97 (d, J=8.8 Hz, 2H), 7.49 (d, J=8.0 Hz, 2H), 7.12 (d, J=8.8 Hz, 2H), 2.58 (s, 3H), 2.51 (s, 9H).

(410) Synthesis of Compound 34-3

(411) ##STR00216##

(412) At 0° C. and under the protection of nitrogen gas, furan (5.60 g, 82.26 mmol, 5.98 mL, 5.55 eq) was added dropwisely to a solution of compound 34-2 (8 g, 14.83 mmol, 1 eq) in toluene (20 mL), and then lithium bis(trimethylsilyl)amide (1 M, 14.82 mL) was slowly added dropwisely. After the completion of the dropwise addition, the mixture was stirred at 0° C. under the protection of nitrogen gas for 3 hours. The reaction mixture was cooled to 0° C., and a saturated ammonium chloride solution (4 mL) was added to quench the reaction. The mixture was extracted with ethyl acetate (3×5 mL). The above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a flash chromatography column to give compound 34-3. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.21 (s, 1H), 8.19 (s, 1H), 7.48-7.50 (m, 1H), 7.11-7.13 (m, 1H), 7.06-7.08 (m, 1H), 5.82-5.84 (m, 2H).

(413) Synthesis of Compound 34-4

(414) ##STR00217##

(415) Under the protection of nitrogen gas, a solution of sodium borohydride (12.00 mg, 317.18 μmol, 1 eq) in water (1 mL) was slowly added dropwisely to a suspension of compound 34-3 (60 mg, 317.18 μmol, 1 eq) and Pd/C (5 mg, 10% purity) in methanol (2 mL). The mixture was stirred at 25° C. under the protection of nitrogen gas for 1 hour. The reaction mixture was filtered with a funnel containing diatomaceous earth. The filtrate was directly concentrated under reduced pressure to give compound 34-4, which was directly used in the next step without the need for any further purification. LCMS (ESI) m/z: 162.1 (M+1).

(416) Synthesis of Compound 34-5

(417) ##STR00218##

(418) Compound 1-11 (35 mg, 87.31 μmol, 1.00 eq) and compound 34-4 (17 mg, 105.46 μmol, 1.21 eq) were successively added to a solution of HATU (35.00 mg, 92.05 μmol, 1.05 eq) in anhydrous N,N-dimethyl formamide (2 mL), and then triethylamine (28.00 mg, 276.71 μmol, 38.51 μL, 3.17 eq) was slowly added dropwisely. The mixture was stirred at 25° C. under the protection of nitrogen gas for 1 hour. Water (3 mL) was added to the reaction mixture to quench the reaction, The mixture was separated into two phases. The aqueous phase was extracted with methylene chloride (3×5 mL). The above organic phases were combined and washed with a saturated sodium bicarbonate solution (5 mL) and a saturated saline solution (5 mL). The organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The obtained crude product was purified with a thin-layer chromatography plate to give compound 34-5. LCMS (ESI) m/z: 544.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.73 (s, 1H), 7.52 (d, J=46.4 Hz, 1H), 7.34 (d, J=8.4 Hz, 2H), 7.26 (d, J=7.6 Hz, 2H), 7.05-7.22 (m, 2H), 5.28 (s, 2H), 4.52-4.56 (m, 1H), 3.68-3.74 (m, 1H), 3.37-3.41 (m, 1H), 2.60 (s, 3H), 2.33 (s, 3H), 1.61 (s, 3H), 1.27 (d, J=6.8 Hz, 2H), 1.19 (d, J=6.8 Hz, 2H).

(419) Synthesis of Compounds 34 and 35

(420) ##STR00219##

(421) Compound 34-5 (20 mg, 36.73 μmol) was purified with a chiral separation (chromatography column: AD (250 mm×30 mm, 10 μm); mobile phase: [0.1% NH.sub.3H.sub.2O EtOH]; B %: 50%-50%) to give compound 34 (Rt=0.735 minutes). LCMS (ESI) m/z: 544.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.79 (s, 1H), 7.58 (s, 1H), 7.34 (d, J=8.4 Hz, 2H), 7.26 (d, J=8.4 Hz, 2H), 7.11-7.15 (m, 1H), 7.05-7.07 (m, 1H), 5.29 (t, J=3.8 Hz, 2H), 4.53-4.57 (m, 1H), 3.67-3.73 (m, 1H), 3.38-3.42 (m, 1H), 2.60 (s, 3H), 2.33 (s, 3H), 1.91-1.96 (m, 2H), 1.61 (s, 3H), 1.25-1.29 (m, 2H).

(422) Compound 35 (Rt=1.300 minutes). LCMS (ESI) m/z: 544.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 8.76 (s, 1H), 7.46 (s, 1H), 7.34 (d, J=8.8 Hz, 2H), 7.26 (d, J=8.8 Hz, 2H), 7.21-7.22 (m, 1H), 7.05-7.07 (m, 1H), 5.28 (s, 2H), 4.53-4.57 (m, 1H), 3.68-3.74 (m, 1H), 3.37-3.42 (m, 1H), 2.60 (s, 3H), 2.33 (s, 3H), 1.94-1.96 (m, 2H), 1.61 (s, 3H), 1.25-1.29 (m, 2H).

(423) ##STR00220##

Example 36

(424) ##STR00221##

(425) Example 36 was synthesized with reference to Example 1.

(426) LCMS (ESI) m/z: 545.1 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 10.10 (s, 1H), 8.08 (s, 1H), 7.73 (d, J=8.0 Hz, 1H), 7.60 (d, J=8.0 Hz, 2H), 7.34 (d, J=8.8 Hz, 2H), 7.25 (d, J=8.8 Hz, 2H), 4.61-4.65 (m, 2H), 3.80-3.86 (m, 1H), 3.48-3.53 (m, 1H), 2.65 (s, 3H), 2.36 (s, 3H), 1.63 (s, 3H).

(427) ##STR00222##

Example 37

(428) ##STR00223##

(429) Synthesis of Compound 37-2

(430) ##STR00224##

(431) At 0° C. and under the protection of nitrogen gas, magnesium methyl bromide (3 M, 9.39 mL, 3 eq) was slowly added dropwisely to a solution of compound 37-1 (2 g, 9.39 mmol, 1 eq) in anhydrous tetrahydrofuran (20 mL). After the completion of the dropwise addition, the mixture was warmed up to 30° C. and stirred under the protection of nitrogen gas for 3 hours. The reaction mixture was cooled to 0° C., and a saturated ammonium chloride solution (10 mL) was slowly added dropwisely to quench the reaction. The mixture was extracted with methylene chloride (3×10 mL). The above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 37-2, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.44 (d, J=2.0 Hz, 1H), 7.39 (dd, J=8.4, 2.0 Hz, 1H), 7.22 (d, J=8.4, 1H), 4.81 (s, 2H), 1.70 (s, 6H).

(432) Synthesis of Compound 37-3

(433) ##STR00225##

(434) Compound 37-2 (2.1 g, 8.57 mmol, 1 eq) was added to a suspension of active manganese peroxide (7.35 g, 84.54 mmol, 9.87 eq) in anhydrous tetrahydrofuran (15 mL). The mixture was stirred at 70° C. for 3 hours. The reaction mixture was cooled to room temperature and filtered. The filtrate concentrated under reduced pressure to give compound 37-3, which was directly used in the next step without the need for any further purification. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.65 (d, J=8.0 Hz, 1H), 7.57 (dd, J=8.0, 1.2 Hz, 1H), 7.49 (d, J=1.2, 1H), 1.59 (s, 6H).

(435) Synthesis of Compound 37-4

(436) ##STR00226##

(437) Tris(dibenzylideneacetone) dipalladium (380.00 mg, 414.98 μmol, 0.1 eq), 4,5-bis(diphenylphosphine)-9,9-dimethylxanthene (240.0 mg, 414.78 μmol, 0.1 eq) and cesium carbonate (2.70 g, 8.29 mmol, 2 eq) were successively added to a solution of compound 37-3 (1 g, 4.15 mmol, 1 eq) and tert-butyl carbamate (700.00 mg, 5.98 mmol, 1.44 eq) in anhydrous 1,4-dioxane (10 mL). The mixture was stirred at 100° C. under the protection of nitrogen gas for 2 hours. The reaction mixture was cooled to room temperature. Water (10 mL) was added to the reaction mixture. The mixture was extracted with methylene chloride (3×10 mL). The above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The obtained crude product was purified with a silica gel column (elution condition: petroleum ether:ethyl acetate=1:1) to give compound 37-4. .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 7.75 (s, 1H), 7.67 (d, J=8.0 Hz, 1H), 7.04 (dd, J=8.4, 1.6 Hz, 1H), 6.79 (br s, 1H), 1.58 (s, 6H), 1.47 (s, 9H).

(438) Synthesis of Compound 37-5

(439) ##STR00227##

(440) Trifluoroacetic acid (7.70 g, 67.53 mmol, 5 mL, 18.73 eq) was slowly added dropwisely to a solution of compound 37-4 (1 g, 3.61 mmol, 1 eq) in anhydrous methylene chloride (10 mL). The mixture was stirred at 30° C. for 1 hour. Water (5 mL) was added to the reaction mixture to quench the reaction. The mixture was adjusted with a saturated sodium bicarbonate solution to pH=7 and then extracted with methylene chloride (3×10 mL). The above organic phases were combined, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 37-5, which was directly used in the next step without the need for any further purification. LCMS (ESI) m/z: 177.8 (M+1).

(441) Synthesis of Compound 37

(442) ##STR00228##

(443) At 0° C., phosphorus oxychloride (140.00 mg, 913.05 μmol, 84.85 μL, 5.23 eq) was slowly added dropwisely to a solution of compound 1-11 (70 mg, 174.62 μmol, 1.00 eq) and compound 37-5 (40 mg, 225.73 μmol 1.29 eq) in pyridine (2 mL). The mixture was stirred at 0° C. under the protection of nitrogen gas for 1 hour. Water (3 mL) was added to the reaction mixture to quench the reaction. The above reaction mixture was adjusted with a hydrochloric acid solution (1 mol/L) to pH=7 and extracted with methylene chloride (3×5 mL). The above organic phases were combined and washed with a saturated sodium bicarbonate solution (5 mL) and a saturated saline solution (5 mL). The organic phase was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The above crude product was purified with a thin-layer chromatography plate to give compound 37. LCMS (ESI) m/z: 560.0 (M+1). .sup.1H NMR (400 MHz, CDCl.sub.3) δppm 9.89 (br s, 1H), 7.92 (s, 1H), 7.62 (d, J=8.4 Hz, 1H), 7.36 (d, J=8.4 Hz, 2H), 7.25-7.30 (m, 3H), 4.57-4.61 (m, 1H), 3.78-3.81 (m, 1H), 3.45-3.50 (m, 1H), 2.62 (s, 3H), 2.35 (s, 3H), 1.64 (s, 3H), 1.54 (s, 3H), 1.53 (s, 3H).

(444) BRD4 Biochemical Activity Test

(445) Experiment Preparation:

(446) 1) BRD4-BD1 and BRD4-BD2 proteins of BPS Company, polypeptide of ANASPEC Company, and test reagent of PerkinElmer Company were used in the experiments;

(447) 2) The TR-FRET experimental principle was used in the experiment to screen compounds.

(448) 3) Relevant control compounds

(449) Experiment Steps:

(450) 1) Preparation of the Compound Plate:

(451) Preparation of the compound plate in the experiment was achieved by Echo:

(452) Compound dilution was completed with Echo, and a three-fold serial dilution to 10 concentrations: 20000, 6666.67, 2222.22, 740.74, 246.91, 82.305, 27.435, 9.145, 3.048, and 1.016 nM, was carried out.

(453) 2) Preparation of Reaction Reagents:

(454) Relevant reaction reagents should be prepared on the day of the experiment:

(455) a) Formulation of 1× assay buffer;

(456) b) Formulation of 3× component solution for the experiment:

(457) 1. The reagent was taken out and placed on ice to naturally melt for later use;

(458) 2. “Solution A” (protein solution), “solution B” (polypeptide solution), and “solution C” (test reagent solution) used in the experiment were formulated with the 1× assay buffer, and during the formation of the 3× solution from the components used in the experiment reaction system, the amounts of solutions A, B and C must be enough for the required amounts in the experiment.

(459) 3) Experimental Operation Steps:

(460) The experiment plate was a plate that contained a gradient concentration of the compound and a corresponding DMSO solution and was prepared with ECHO before the experiment:

(461) a) The experiment plate was taken out, and 5 μL/well of “solution A” (protein solution) was added to columns 2-23 of the experiment plate, then 5 μL/well of 1× assay buffer was added to columns 1 and 24 of the experiment plate, and columns 1 and 24 were used as Min control in the experiment system;

(462) b) Centrifugation at 1000 rpm was carried out for 30 seconds;

(463) c) The plate was incubated at 23° C. for 20 minutes;

(464) d) After 20 minutes of incubation, 5 μL/well of “solution B” (polypeptide solution) was added to columns 1-24 of the experiment plate;

(465) e) Centrifugation at 1000 rpm was carried out for 30 seconds;

(466) f) The plate was incubated at 23° C. for 20 minutes;

(467) g) After 20 minutes of incubation, 5 μL/well of “solution C” (test reagent solution) was added to columns 1-24 of the experiment plate;

(468) h) Centrifugation at 1000 rpm was carried out for 30 seconds;

(469) i) The plate was incubated at 23° C. for 40 minutes;

(470) j) The experiment plate was placed on EnVision to read the plate.

(471) 4) Data Analysis:

(472) a) The corresponding Max control and Min control of each experiment plate were used to convert to the Z′ value of the experiment plate, and the Z′ value of each plate was ensured to be >0.5;

(473) b) The IC.sub.50 value was calculated from the signal of the control compound by XLFIT5, and ensured to be maintained within 3 times of the average value of historical data. The results were shown in Table 1.

(474) TABLE-US-00001 TABLE 1 IC.sub.50 test results of the BRD4 test Compound BRD4 Binding (BD1, BD2), IC.sub.50(nM) 1  65, 11 2  49, 10 3  87, 15 4 129, 14 5 83, 9 6 327, 90 7 100, 21 8  51, 11 9  49, 11 10  81, 16 11  49, 14 12  40, 12 13 50, 8 14 69, 8 15  91, 11 16  60, 12 17 24, 7 18 23, 8 19 49, 8 20 169, 11 21 522, 34 22 199, 10 23 158, 22 24 206, 17 25  79, 20 26 112, 30 27 55, 9 28  60, 15 29  74, 16 30 51, 9 31 37, 5 32  66, 10 33 248, 19 34 117, 11 35 155, 12 36 126, 10 37 148, 11

(475) Conclusion: The compounds of the present invention had significant BET Bromodomain inhibitory activities.

(476) In Vivo Pharmacodynamics Study of Compound 32 on Human Breast Cancer MDA-MB-231_luc Cell Subcutaneous Xenograft Tumor Model

(477) 1. Experiment Design

(478) TABLE-US-00002 TABLE 2 Formulation method of the substance to be tested Packaging or starting Concentration Storage Compound concentration Formulation method (mg/mL) condition Medium — 5% DMSO + 40% PEG400 + 10% — 4° C. Kolliphor ® HS 15 + 45% H.sub.2O Compound 511 mg 126.52 mg of Compound 32 was added to a 5 4° C. 32 brown bottle, and then 1.26 mL of DMSO 50 mg/kg, was added thereto. The mixture was stirred BID by vortex to a homogeneous solution. 10.080 mL of PEG400 and 2.52 mL of solutol were added and stirred by vortex to a homogeneous solution, and then 11.340 mL of H.sub.2O was added and stirred by vortex to obtain a solution containing Compound 32 at a concentration of 5 mg/mL.

(479) TABLE-US-00003 TABLE 3 Animal grouping and dosage regimen for in vivo pharmacodynamic experiments Administration Number of Compound Dosage volume parameter Administration Administration Group animals treatment (mg/kg) (μL/g) route frequency 1 6 Vehicle — 10 PO BID × 21 days 2 6 Compound 32 50 10 PO BID × 21 days

(480) 2. Experiment Material

(481) 2.1 Experiment Animals

(482) Species: mouse

(483) Strain: BALB/c nude mouse

(484) Week-age and weight: 6-8 weeks of age, 18-22 grams of body weight

(485) Sex: Female

(486) Supplier: Shanghai Sippr-BK laboratory animal Co. Ltd.

(487) 3. Experiment Methods and Steps

(488) 3.1 Cell Culture

(489) Human breast cancer MDA-MB-231_luc cells were cultured in monolayer in vitro and the culture conditions were RPMI-1640 culture medium (supplier: Gibco; article number: 22400-089; manufacturing batch number: 4868546) with 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml streptomycin. The culture was performed at 37° C. in 5% CO.sub.2. Conventional digestion treatment with pancreatin-EDTA for passage was carried out twice a week. When the cells were in the exponential growth phase, the cells were harvested, counted, and inoculated.

(490) 3.2 Tumor Cell Inoculation

(491) 0.2 mL of 10×10.sup.6 MDA-MB-231_luc cells were subcutaneously inoculated into the right-back of each nude mouse (PBS:Matrigel=1:1). The grouping and administration was started when the average tumor volume reached 100-150 mm.sup.3.

(492) 3.3 Tumor Measurement and Experiment Indices

(493) The experiment index was to investigate whether tumor growth was inhibited, delayed or cured. Tumor diameter was measured twice a week with vernier calipers. The equation for calculating the tumor volume was V=0.5×a×b.sup.2, wherein a and b represented the major and minor diameters of the tumor, respectively.

(494) The tumor inhibition effect of the compound was evaluated by TGI (%) or relative tumor proliferation rate T/C (%). TGI (%) reflected the tumor growth inhibition rate. Calculation of TGI (%) was as follows: TGI (%)=[(1−(average tumor volume at the end of administration in a treatment group−average tumor volume at the beginning of administration in this treatment group))/(average tumor volume at the end of treatment in the solvent control group−average tumor volume at the beginning of treatment in the solvent control group)]×100%.

(495) Relative tumor proliferation rate T/C (%) was calculated according to the below equation: T/C %=T.sub.RTV/C.sub.RTV×100% (T.sub.RTV: RTV of the treatment group; C.sub.RTV: RTV of the negative control group). The relative tumor volume (RTV) was calculated according to the results of the tumor measurement. The calculation equation was RTV=V.sub.t/V.sub.0, where V.sub.0 was the average tumor volume measured at the grouping and administration (i.e. d.sub.0), and V.sub.t was the average tumor volume at the time of a certain measurement. T.sub.RTV and C.sub.RTV were obtained from the data on the same day.

(496) At the end of the experiment, the tumor weight would be measured and the T.sub.weight/C.sub.weight percentage would be calculated. T.sub.weight and C.sub.weight represented the tumor weights of the administration group and the medium control group, respectively.

(497) 3.4 Statistical Analysis

(498) Statistical analysis included mean value and standard error (SEM) of the tumor volume of each group at each time point. The treatment group showed the best treatment effect on the 21st day after the administration at the end of the experiment, so the statistical analysis was performed based on this data to evaluate the differences between the groups. The comparison between two groups was analyzed by T-test, and the comparison between three or more groups was analyzed by one-way ANOVA. If the F value was significantly different, the Games-Howell test was applied. If the F value was not significantly different, the Dunnet (2-sided) test was used for analysis. All data analysis was performed with SPSS 17.0. p<0.05 was considered significantly different.

(499) 4. Experiment Conclusion

(500) On the 21st day after administration, for the test compound 32, the tumor growth inhibition rate TGI=54.85%, T/C=52.99%, p<0.05; there was no significant change in body weight of the animals, and they were well tolerated.

(501) In Vivo Pharmacodynamics Study of Compound 32 on Human Prostatic Cancer PC-3 Cell Subcutaneous Xenograft Tumor Model

(502) 1. Experimental Design

(503) The formulation method of the test substance was the same as in Table 2, and the animal grouping and the dosage regimen were the same as in Table 3.

(504) 2. Experiment Material

(505) 2.1 Experiment Animals

(506) Species: mouse

(507) Strain: BALB/c nude mouse

(508) Week-age and weight: 6-8 weeks of age, 18-22 grams of body weight

(509) Sex: male

(510) Supplier: Shanghai Sippr-BK laboratory animal Co. Ltd.

(511) 3. Experiment Methods and Steps

(512) 3.1 Cell Culture

(513) Human prostatic cancer PC-3 cells were cultured in monolayer in vitro, and the culture conditions were F-12K culture medium (supplier: Gibco; article number: 21127-022; manufacturing batch number: 1868870) with 10% fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin. The culture was performed at 37° C. in 5% CO.sub.2. Conventional digestion treatment with pancreatin-EDTA for passage was carried out twice a week. When the cells were in the exponential growth phase, the cells were harvested, counted, and inoculated.

(514) 3.2 Tumor Cell Inoculation

(515) 0.1 mL of 10×10.sup.6 PC-3 cells were subcutaneously inoculated into the right-back of each nude mouse. The grouping and administration was started when the average tumor volume reached 100-150 mm.sup.3.

(516) 3.3 Tumor Measurement, Experiment Indices, and Statistical Analysis were the Same as MDA-MB-231 Model

(517) 4. Experiment Conclusion

(518) On the 21st day after administration, compared with the solvent control group, the test compound 32 had a significant tumor inhibition effect (T/C=44.63%, TGI=58.4%, p=0.033); the animals were well tolerated.

(519) In Vivo Pharmacokinetics Test of Compound 32 in Mice

(520) Female Balb/c mice were used as test animals. The compound 32 was administrated intravenously and intragastrically to mice, then the drug concentrations in the plasma at different time points were determined by the LC/MS/MS method. The in vivo pharmacokinetic behavior of compound 32 in mice was studied, and its pharmacokinetic characteristics were evaluated.

(521) 1. Experiment Protocol

(522) 1.1 Experiment drug: Compound 32

(523) 1.2 Experiment animals: Sixteen healthy adult female Balb/c mice were divided into four groups according to the principle of similar body weight, with four mice in each group. The animals were purchased from Shanghai Lingchang BioTech Co., Ltd. of Shanghai SLAC Laboratory Animal Co., Ltd., and the animal production license number was SCXK (Shanghai) 2013-0018.

(524) 1.3 Drug Formulation

(525) An appropriate amount of the sample was taken, 5% final volume of DMSO was added, and then 95% final volume of 20% HP-β-CD was added. The mixture was ultrasonically stirred to obtain a 0.5 mg/mL clear solution. After filtration, it was used for the intravenous administration.

(526) An appropriate amount of the sample was taken, and dissolved in a 0.5% sodium carboxymethyl cellulose solution. The mixture was ultrasonically stirred to obtain a 0.5 mg/mL homogeneous suspension, which was used for the intragastric administration.

(527) 1.4 Administration

(528) Eight female Balb/c mice were divided into two groups. After fasting overnight, the first group was administered intravenously with the administration volume of 2.5 mL/kg and the dosage of 1 mg/kg. The second group was administered intragastrically with the administration volume of 5 mL/kg and the dosage of 2 mg/kg.

(529) 2. Operation

(530) After the female Balb/c mice were intravenously administrated, 30 μL of blood was taken from different mice at each time point of 0.0833, 0.25, 0.5, 1, 2, 4, 8 and 24 hours, and placed in test tubes containing 2 μL of EDTA-K.sub.2; and after the female Balb/c mice were intragastrically administrated, 30 μL of blood was taken at an alternative location at each time point of 0.25, 0.5, 1, 2, 4, 8 and 24 hours, and placed in test tubes containing 2 μL of EDTA-K.sub.2. The tube was centrifuged at 3000 g for 15 minutes to separate the plasma, and the separated plasma was stored at −60° C. Animals could take food 2 hours after the administration.

(531) The LC/MS/MS method was used to measure the content of the compound to be tested in the plasma after the intravenous and intragastric administration to the mice. The linear range of the method was 2.00-6000 nmol/L; the plasma samples were analyzed after the protein precipitation by acetonitrile treatment. The results of the pharmacokinetic parameters were shown in Table 4.

(532) TABLE-US-00004 TABLE 4 Results of pharmacokinetic parameters Compound 32 Administration mode Intravenous Intragastric Administration dosage 1 mg/kg 3 mg/kg Drug concentration C.sub.max (nM) — 930 in blood Time to peak T.sub.max (h) — 1.00 Half life T.sub.1/2 (h) 1.09 1.47 Apparent Vdss (L/kg) 1.19 — distribution volume Clearance rate Cl (mL/min/kg) 12.5 — Curve area (0-t) AUC.sub.0-last (nM .Math. h) 2490 2740 Curve area (0-inf) AUC.sub.0-inf (nM .Math. h) 2502 2818 Bioavailability Bioavailability (%) — 37.50% “—”: Not available.

(533) Experiment conclusion: Compound 32 had low pharmacokinetic clearance and good absorption.

(534) In Vivo Anti-Tumor Effect of Compound 32 in MC38 Mouse Colon Cancer Cell Animal Transplantation Tumor Model

(535) 1. Experimental Design

(536) TABLE-US-00005 Group 1 2 3 4 Number of animals 10 10 10 10 Substance to be tested Medium control Compound 32 Compound 32 Compound 32 Dosage — 15 25 50 mg/kg Administration volume 10 10 10 10 mL/kg Administration route p.o. p.o. p.o. p.o. Administration frequency BID × 20 days BID × 20 days BID × 20 days BID × 20 days and cycle

(537) 2. Experiment Material

(538) 2.1 Experiment Animals

(539) Species: mouse

(540) Strain: C57BL6 mouse

(541) Week-age and weight: 6-7 weeks of age, 16-20 grams of body weight

(542) Sex: Female

(543) Supplier: Shanghai SLAC Laboratory Animal Co., Ltd.

(544) 3. Experiment Methods and Steps

(545) 3.1 Cell Culture

(546) Mouse colon cancer MC38 cells (OBiO Technology (Shanghai) Corp., Ltd.) were cultured in monolayer in vitro, and the culture conditions were DMEM culture medium (Gibco; article number: 12100) with 10% fetal bovine serum. The culture was performed at 37° C. in 5% CO.sub.2 in an incubator. Conventional digestion treatment with 0.25% pancreatin-EDTA for passage was carried out. When the cells were in the exponential growth phase and the saturation was 80%-90%, the cells were harvested, counted, and inoculated.

(547) 3.2 Tumor Cell Inoculation

(548) 0.1 mL of 2×10.sup.5 MC38 cells were subcutaneously inoculated into the right-back of each mouse. The random grouping and administration was carried out according to the tumor volume when the average tumor volume reached about 70 mm.sup.3.

(549) 3.3 Tumor Measurement

(550) Tumor diameter was measured twice a week with vernier calipers. The equation for calculating tumor volume was V=0.5×a×b.sup.2, wherein a and b represented the major and minor diameters of the tumor, respectively.

(551) The tumor inhibition effect of the compound was evaluated by TGI (%) or relative tumor proliferation rate T/C (%). Relative tumor proliferation rate T/C (%)=T.sub.RTV/C.sub.RTV×100% (T.sub.RTV: RTV of the treatment group; C.sub.RTV: RTV of the negative control group). The relative tumor volume (RTV) was calculated according to the results of the tumor measurement. The calculation equation was RTV=V.sub.t/V.sub.0, where V.sub.0 was the average tumor volume measured at the grouping and administration (i.e. D.sub.0), and V.sub.t was the average tumor volume at the time of a certain measurement. T.sub.RTV and C.sub.RTV were obtained from the data on the same day.

(552) TGI (%) reflected the tumor growth inhibition rate. TGI (%)=[(1−(average tumor volume at the end of administration in a treatment group−average tumor volume at the beginning of administration in this treatment group))/(average tumor volume at the end of treatment in the solvent control group−average tumor volume at the beginning of treatment in the solvent control group)]×100%.

(553) At the end of the experiment, the tumor weight would be measured and the T.sub.weight/C.sub.weight percentage would be calculated. T.sub.weight and C.sub.weight represented the tumor weights of the administration group and the medium control group, respectively.

(554) 3.4 Statistical Analysis

(555) Statistical analysis was performed using SPSS software based on the tumor volume and the tumor weight at the end of the experiment. The comparison between two groups was analyzed by t-test, and the comparison between three or more groups was analyzed by one-way ANOVA. If the variance was homogeneous (the F value was not significantly different), the LSD method was used for analysis. If the variance was not homogeneous (the F value was significantly different), the Games-Howell method was used for the test. p<0.05 was considered significantly different.

(556) 4. Experiment Conclusion

(557) On the 20th day after administration, for the test compound 32, for the 15 mg/kg administration group: the relative tumor proliferation rate T/C=33.68%, the tumor growth inhibition rate TGI=68.81%, p<0.0001; for the 25 mg/kg administration group: the relative tumor proliferation rate T/C=27.59%, TGI=75.21%, p<0.0001; and for the 50 mg/kg administration group: T/C=10.04%, TGI=93.46%, p<0.0001. Significant tumor inhibition effects were shown in each administration group of animals with good tolerance.