Cold sore treatment formulation and related method of application-liquid patch for treatment of viral lesions

Abstract

A formulation of a discrete liquid patch for the treatment of cold sores or viral lesions, including a combination of the adhesive layer and an active compound/ingredient such as antiviral agents, and/or amino acids, and/or minerals/vitamins. The adhesive layer of the discrete patch is substantially free of hydrocolloid particles. The adhesive layer does not include any backing layer to maintain the compliance of the patch with a thickness ranging from 5 microns to 1,300 microns. Wherein, the discrete patch of the invention is configured to apply to a viral lesion to maintain in contact therewith for a time effective to substantially complete re-epithelialization of the lesion.

Claims

1. A formulation of a liquid discreet patch for the treatment of cold sores consisting of a. a translucent liquid adhesive consisting of at least one non-active ingredient and characterized in that the water content is less than 5% by weight, b. at least one active ingredient selected from the group consisting of Docosonol, L-Lysine, Penciclovir, and/or Acyclovir, and c. an anti-septic compound whereas, said translucent liquid adhesive is mixed with said active ingredient and applied to form a single layer whereas the formulation does not comprise a backing layer.

2. The formulation of a liquid discreet patch according to claim 1, wherein the translucent liquid adhesive consists of an alcohol base.

3. The formulation of a liquid discreet patch according to claim 1, wherein the non-active ingredient is selected from the group consisting of Nitrocellulose, Tea Tree Oil, Camphor, Dimethyl Sulfoxide (DMSO), Amyl Acetate, Ethyl Acetate, Ethyl Alcohol and Isopropyl Alcohol.

4. The formulation of a liquid discreet patch according to claim 1, wherein the active ingredient consists of at least one of Docosanol and L-lysine.

5. The formulation of a liquid discreet patch according to claim 1, wherein the anti-septic compound is selected from the group consisting of Benzalkonium Chloride and Benzethonium Chloride.

6. The formulation of a liquid discreet patch according to claim 1, wherein the active ingredient consists of Docosanol 4% by weight, and the adhesive consists of Tea Tree Oil 1% by weight, Nitrocellulose 15% by weight, Camphor 1% by weight, Amyl Acetate 28% by weight, Ethyl Acetate 28% by weight, Ethyl Alcohol 15% by weight and Isopropyl Alcohol 8% by weight.

7. The formulation of a liquid discreet patch according to claim 1, wherein the active ingredient consists of Docosanol 4% by weight, and the adhesive consists of Tea Tree Oil 1% by weight, Nitrocellulose 15% by weight, Camphor 1% by weight, Amyl Acetate 28% by weight, Ethyl Acetate 28% by weight, Ethyl Alcohol 15% by weight, Isopropyl Alcohol 8% by weight and Benzethonium Chloride 0.2% by weight.

8. The formulation of a liquid discreet patch according to claim 1, wherein the active ingredient consists of L-lysine 10% by weight, and the adhesive consists of Tea Tree Oil 1% by weight, Nitrocellulose 10% by weight, Camphor 1° A) by weight, Amyl Acetate 19% by weight, Ethyl Acetate 19% by weight, Ethyl Alcohol 10% by weight and Dimethyl Sulfoxide 30% by weight.

9. The formulation of a liquid discreet patch according to claim 1, wherein the active ingredient consists of Docosanol 4% by weight and L-lysine 6% by weight, and the adhesive consists of Tea Tree Oil 1% by weight, Nitrocellulose 10% by weight, Camphor 1% by weight, Amyl Acetate 19% by weight, Ethyl Acetate 19% by weight, Ethyl Alcohol 10% by weight and Dimethyl Sulfoxide 30% by weight.

10. The formulation of a liquid discreet patch according to the claim 1, wherein the active ingredient consists of Penciclovir 1% by weight, and the adhesive consists of Tea Tree Oil 1% by weight, Nitrocellulose 15% by weight, Camphor 1% by weight, Amyl Acetate 28% by weight, Ethyl Acetate 28% by weight, Ethyl Alcohol 15% by weight and Isopropyl Alcohol 11% by weight.

11. The formulation of a liquid discreet patch according to claim 1, wherein the active ingredient consists of Acyclovir 5% by weight, and the adhesive consists of Tea Tree Oil 1% by weight, Nitrocellulose 15% by weight, Camphor 1% by weight, Amyl Acetate 28% by weight, Ethyl Acetate 28% by weight, Ethyl Alcohol 15% by weight and Isopropyl Alcohol 7% by weight.

12. A method of use of a liquid discreet patch of claim 1, the method comprising: (a) positioning the liquid discreet patch on to a lesion surface and maintaining contact between the liquid discreet patch with the lesion surface for at least 2 hours, but no longer than 12 hours, and (b) delivering subsequent patches and maintaining said subsequent patches in contact with the lesion surface, until such time as re-epithelialization of the lesion surface is completed.

13. The formulation of a liquid discreet patch according to claim 1, wherein the liquid discreet patch is characterized by being applied as a liquid and once dried, forming a translucent film barrier with a thickness of between 5 and 1300 microns.

14. The formulation of a liquid discreet patch according to claim 1, wherein the liquid discreet patch is characterized as being flexible and porous with the ability to allow moisture to pass through and evaporate, maintaining a dry surface that is unaffected by movement in regular wear.

Description

DETAILED DESCRIPTION

(1) The present invention relates to a composition/formulation for treatment of cold sores/viral lesions. It intended to improve healing times, reduce discomfort and prevent secondary/spread of infection.

(2) More specifically, the present invention relates to formulation of the liquid discreet patch comprising of a translucent liquid adhesive, at least one active ingredient, at least one non-active ingredient, and an anti-septic compound. The translucent liquid adhesive is composed of alcoholic base, which includes the non-active ingredient. Whereas, the liquid discreet patch does not require additional backing layer.

(3) According to a preferred embodiment, the formulation comprises, the translucent liquid adhesive, which is composed of an alcohol base due to the anti-septic qualities. However, for the purpose of active ingredient delivery, hydrophilic and silicon bases can also be use. The alcohol base composition of the translucent liquid adhesive further includes the at least one non-active ingredient is selected from the group consisting of Nitrocellulose, Tea Tree Oil, Camphor, Dimethyl Sulfoxide (DMSO), Amyl Acetate, Ethyl Acetate, Ethyl Alcohol and Isopropyl Alcohol. More specifically, composition comprises suitable weights of total formulation; Nitrocellulose 0% to 20%, Tea Tree Oil suitable 0% to 10%, Camphor 0% to 10%, Amyl Acetate 0% to 20%, Ethyl Acetate 0% to 30%, Ethyl Alcohol 0% to 30%, Isopropyl Alcohol 0% to 20%, Dimethyl Sulfoxide (DMSO) 0% to 45%. In certain situations, the non-active ingredients can also be combined with the active ingredients during preparation of formulation.

(4) According to another preferred embodiment, the formulation of the present invention also contains at least one the active ingredient selected from group consisting of anti-viral, amino acids and vitamin ingredients such as Docosanol, L-lysine, Acyclovir, Penciclovir, Zinc, and Magnesium. More specifically, composition comprises suitable weights of total formulation; Docosanol 0% to 10%, L-lysine 0% to 10%, Penciclovir 0% to 5%, Acyclovir 0% to 5%, Magnesium 0% to 10% and Zinc 0% to 10%. The active ingredient can be mixed with the anti-septic compound and the non-active ingredients during preparation of formulation.

(5) According to the present invention, the formulation of the liquid discreet patch may further include at least one additional antiseptic compound selected from the group consisting of Benzethonium Chloride and Benzalkonium Chloride. More specifically, composition comprises suitable weights of total formulation; Benzethonium Chloride 0% to 5% and Benzalkonium Chloride 0% to 5%.

(6) According to the present invention, the liquid discreet patch can be applied on to the surface of the lesion by positioning the liquid discreet patch on to a lesion surface and maintaining contact between the liquid discreet patch with the lesion surface for at least 2 hours, but no longer than 12 hours. Subsequent patches can be applied and maintained in contact with the lesion surface, until such time as re-epithelialization of the lesion surface is completed. More specifically, the liquid discreet patch can be applied as a liquid over the affected dermal region, typically of the mouth and facial area. The liquid discreet patch is designed to dry and form a porous, translucent film barrier that allows moisture to pass through the patch and evaporate to maintain adhesion and a dry surface area. The liquid discreet patch can also help prevent the growth and spread of the virus by acting as a barrier, reducing interaction and reducing/eliminating discomfort.

(7) In accordance with the invention, the liquid discreet patch is characterized by consisting of a single layer with absence of any additional secondary or tertiary structures or elements such as a backing layers and wound dressings. The liquid discreet patch delivery the active ingredients at the site of the cold sore outbreaks or lesions.

(8) Accordingly, according to a preferred embodiment, the formulation of the present invention where the thickness of the liquid discreet patch is between 5 and 1300 microns to maintain translucent nature and low visibility of the patch after application on to the surface of the lesion.

(9) According to a preferred embodiment, the liquid discreet patch can be applied to a clean surface area as soon as the afflicted identifies an oncoming outbreak and should remain in contact with the cold sore/lesion for a time up to 12 hours but no less than 2 hours. The patch can be maintained in contact with the lesion surface until re-epithelization has been completed. To achieve an effect the patch can be replaced concurrently with another patch until completion re-epithelization.

(10) In accordance with the invention, the liquid discreet patch can be prepared by a two-step process at room temperature. The First step consists of the mixing of one or more the non-active ingredients while stirring at room temperature for about 30 minutes until it gets dissolves with mixing speed is 300-1000 rpm. The second step consist of the mixing of active ingredients and anti-septic compounds and/or non-active ingredients separately in the order determined by the formulation at room temperature with a mixing speed of 300-1000 rpm. A total mixing time is 60 minutes or until all the ingredients dissolve completely to obtained a transparent solution.

EXAMPLES

(11) Several Examples are set forth below. The claims should not be considered to be limited to the details thereof.

Example 1

(12) (The liquid discreet patch with Docosanol 4%)

(13) TABLE-US-00001 Components Quantity (% w/w) Docosanol  4% Tea Tree Oil  1% Nitrocellulose 15% Camphor  1% Amyl Acetate 28% Ethyl Acetate 28% Ethyl Alcohol 15% Isopropyl Alcohol  8%
Process of Preparation:
Step 1: 15.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 28 mL of amyl acetate while stirring at room temperature for 30 minutes or until the nitrocellulose dissolves completely in amyl acetate; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: camphor (1.0 g), tea tree oil (1.0 g), ethyl acetate (28.0 g), Docosanol (4.0 g), ethyl alcohol (15.0 g), and isopropyl alcohol (8.0 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 60 minutes or until all the ingredients dissolve completely.

Example 2

(14) (The liquid discreet patch with Docosanol 4% with an Anti-septic additive; Benzethonium Chloride 0.2%)

(15) TABLE-US-00002 Components Quantity (% w/w) Docosanol  4% Benzethonium Chloride 0.2%  Tea Tree Oil  1% Nitrocellulose 15% Camphor  1% Amyl Acetate 28% Ethyl Acetate 28% Ethyl Alcohol 15% Isopropyl Alcohol 7.8% 
Process of Preparation:
Step 1: 15.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 28 mL of amyl acetate while stirring at room temperature for 30 minutes or until the nitrocellulose dissolves completely in amyl acetate; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: camphor (1.0 g), tea tree oil (1.0 g), ethyl acetate (28.0 g), Docosanol (4.0 g), ethyl alcohol (15.0 g), isopropyl alcohol (7.8 g), and Benzethonium chloride (0.2 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 60 minutes or until all the ingredients dissolve completely.

Example 3

(16) (The liquid discreet patch with Docosanol 4% with an Anti-septic additive; Benzalkonium Chloride 0.2%)

(17) TABLE-US-00003 Components Quantity (% w/w) Docosanol  4% Benzalkonium Chloride 0.2%  Tea Tree Oil  1% Nitrocellulose 15% Camphor  1% Amyl Acetate 28% Ethyl Acetate 28% Ethyl Alcohol 15% Isopropyl Alcohol 7.8% 
Process of Preparation:
Step 1: 15.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 28 mL of amyl acetate while stirring at room temperature for 30 minutes or until the nitrocellulose dissolves completely in amyl acetate; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: camphor (1.0 g), tea tree oil (1.0 g), ethyl acetate (28.0 g), Docosanol (4.0 g), ethyl alcohol (15.0 g), isopropyl alcohol (7.8 g), and Benzalkonium Chloride (0.2 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 60 minutes or until all the ingredients dissolve completely.

Example 4

(18) (The liquid discreet patch with Docosanol 4% in combination with L-lysine)

(19) TABLE-US-00004 Components Quantity (% w/w) Docosanol  4% L-lysine  6% Tea Tree Oil  1% Nitrocellulose 10% Camphor  1% Amyl Acetate 19% Ethyl Acetate 19% Ethyl Alcohol 10% Dimethyl Sulfoxide 30%
Process of Preparation:
Step 1: 10.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 30 mL of dimethyl sulfoxide while stirring at room temperature for 60 minutes or until the nitrocellulose dissolves completely in dimethyl sulfoxide; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: L-lysine (6.0 g), ethyl alcohol (10.0 g), camphor (1.0 g), tea tree oil (1.0 g), amyl acetate (19.0 g), and ethyl acetate (19.0 g), Docosanol (4.0 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 120 minutes or until all the ingredients dissolve completely.

Example 5

(20) (The liquid discreet patch with L-lysine 10%)

(21) TABLE-US-00005 Components Quantity (% w/w) L-lysine 10% Tea Tree Oil  1% Nitrocellulose 10% Camphor  1% Amyl Acetate 19% Ethyl Acetate 19% Ethyl Alcohol 10% Dimethyl Sulfoxide 30%
Process of Preparation:
Step 1: 10.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 30 mL of dimethyl sulfoxide while stirring at room temperature for 60 minutes or until the nitrocellulose dissolves completely in dimethyl sulfoxide; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: L-lysine (10.0 g), ethyl alcohol (10.0 g), camphor (1.0 g), tea tree oil (1.0 g), amyl acetate (19.0 g), and ethyl acetate (19.0 g), at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 120 minutes or until all the ingredients dissolve completely.

Example 6

(22) (The liquid discreet patch with Docosanol 4% in combination with L-lysine 6% and the anti-septic compound; Benzethonium Chloride 0.2%)

(23) TABLE-US-00006 Components Quantity (% w/w) Docosanol  4% L-lysine  6% Benzethonium Chloride 0.2%  Tea Tree Oil  1% Nitrocellulose 10% Camphor  1% Amyl Acetate 19% Ethyl Acetate 19% Ethyl Alcohol 10% Dimethyl Sulfoxide 29.8%.sup. 
Process of Preparation:
Step 1: 10.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 29.8 mL of dimethyl sulfoxide while stirring at room temperature for 60 minutes or until the nitrocellulose dissolves completely in dimethyl sulfoxide; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: L-lysine (6.0 g), ethyl alcohol (10.0 g), camphor (1.0 g), tea tree oil (1.0 g), amyl acetate (19.0 g), ethyl acetate (19.0 g), Docosanol (4.0 g), and Benzethonium Chloride 0.2% at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 120 minutes or until all the ingredients dissolve completely.

Example 7

(24) (The liquid discreet patch with Docosanol 4% in combination with L-lysine 6% and the anti-septic compound; Benzalkonium Chloride 0.2%)

(25) TABLE-US-00007 Components Quantity (% w/w) Docosanol  4% L-lysine  6% Benzalkonium Chloride 0.2%  Tea Tree Oil  1% Nitrocellulose 10% Camphor  1% Amyl Acetate 19% Ethyl Acetate 19% Ethyl Alcohol 10% Dimethyl Sulfoxide 29.8%.sup. 
Process of Preparation:
Step 1: 10.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 29.8 mL of dimethyl sulfoxide while stirring at room temperature for 60 minutes or until the nitrocellulose dissolves completely in dimethyl sulfoxide; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: L-lysine (6.0 g), ethyl alcohol (10.0 g), camphor (1.0 g), tea tree oil (1.0 g), amyl acetate (19.0 g), ethyl acetate (19.0 g), Docosanol (4.0 g), and Benzalkonium 0.2% at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 120 minutes or until all the ingredients dissolve completely. A transparent solution is obtained.

Example 8

(26) (The liquid discreet patch with Penciclovir 1%)

(27) TABLE-US-00008 Components Quantity (% w/w) Penciclovir  1% Tea Tree Oil  1% Nitrocellulose 15% Camphor  1% Amyl Acetate 28% Ethyl Acetate 28% Ethyl Alcohol 15% Isopropyl Alcohol 11%
Process of Preparation:
Step 1: 15.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 28 mL of amyl acetate while stirring at room temperature for 30 minutes or until the nitrocellulose dissolves completely in amyl acetate; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: camphor (1.0 g), tea tree oil (1.0 g), ethyl acetate (28.0 g), Penciclovir (1.0 g), ethyl alcohol (15.0 g), and isopropyl alcohol (11.0 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 60 minutes or until all the ingredients dissolve completely.

Example 9

(28) (The liquid discreet patch with Penciclovir 1% with an antiseptic additive; Benzalkonium Chloride 0.2%)

(29) TABLE-US-00009 Components Quantity (% w/w) Penciclovir  1% Benzalkonium Chloride 0.2%  Tea Tree Oil  1% Nitrocellulose 15% Camphor  1% Amyl Acetate 28% Ethyl Acetate 28% Ethyl Alcohol 15% Isopropyl Alcohol 10.8%.sup. 
Process of Preparation:
Step 1: 15.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 28 mL of amyl acetate while stirring at room temperature for 30 minutes or until the nitrocellulose dissolves completely in amyl acetate; mixing speed is 300-1000 rpm Step 1: Other ingredients are mixed and dissolved separately in the following order: camphor (1.0 g), tea tree oil (1.0 g), ethyl acetate (28.0 g), Penciclovir (1.0 g), ethyl alcohol (15.0 g), isopropyl alcohol (10.8 g), and Benzalkonium (0.2 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 60 minutes or until all the ingredients dissolve completely.

Example 10

(30) (The liquid discreet patch with Penciclovir 1% with an antiseptic additive; Benzethonium Chloride 0.2%)

(31) TABLE-US-00010 Components Quantity (% w/w) Penciclovir  1% Benzethonium Chloride 0.2%  Tea Tree Oil  1% Nitrocellulose 15% Camphor  1% Amyl Acetate 28% Ethyl Acetate 28% Ethyl Alcohol 15% Isopropyl Alcohol 10.8%.sup. 
Process of Preparation:
Step 1: 15.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 28 mL of amyl acetate while stirring at room temperature for 30 minutes or until the nitrocellulose dissolves completely in amyl acetate; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: camphor (1.0 g), tea tree oil (1.0 g), ethyl acetate (28.0 g), Penciclovir (1.0 g), ethyl alcohol (15.0 g), isopropyl alcohol (10.8 g), and Benzethonium Chloride (0.2 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 60 minutes or until all the ingredients dissolve completely.

Example 11

(32) (The liquid discreet patch with Acyclovir 5%)

(33) TABLE-US-00011 Components Quantity (% w/w) Acyclovir  5% Tea Tree Oil  1% Nitrocellulose 15% Camphor  1% Amyl Acetate 28% Ethyl Acetate 28% Ethyl Alcohol 15% Isopropyl Alcohol  7%
Process of Preparation:
Step 1: 15.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 28 mL of amyl acetate while stirring at room temperature for 30 minutes or until the nitrocellulose dissolves completely in amyl acetate; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: camphor (1.0 g), tea tree oil (1.0 g), ethyl acetate (28.0 g), Acyclovir (5.0 g), ethyl alcohol (15.0 g), and isopropyl alcohol (7.0 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 60 minutes or until all the ingredients dissolve completely.

Example 12

(34) (The liquid discreet patch with Acyclovir 4.8% with an antiseptic additive; Benzethonium Chloride 0.2%)

(35) TABLE-US-00012 Components Quantity (% w/w) Acyclovir 4.8%  Benzethonium Chloride 0.2%  Tea Tree Oil  1% Nitrocellulose 15% Camphor  1% Amyl Acetate 28% Ethyl Acetate 28% Ethyl Alcohol 15% Isopropyl Alcohol  7%
Process of Preparation:
Step 1: 15.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 28 mL of amyl acetate while stirring at room temperature for 30 minutes or until the nitrocellulose dissolves completely in amyl acetate; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: camphor (1.0 g), tea tree oil (1.0 g), ethyl acetate (28.0 g), Acyclovir (4.8 g), ethyl alcohol (15.0 g), isopropyl alcohol (7.0 g), and Benzethonium Chloride (0.2 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 60 minutes or until all the ingredients dissolve completely. A transparent solution is obtained.

Example 13

(36) (The liquid discreet patch with Acyclovir 4.8% with an antiseptic additive; Benzalkonium Chloride 0.2%)

(37) TABLE-US-00013 Components Quantity (% w/w) Acyclovir 4.8%  Benzalkonium Chloride 0.2%  Tea Tree Oil  1% Nitrocellulose 15% Camphor  1% Amyl Acetate 28% Ethyl Acetate 28% Ethyl Alcohol 15% Isopropyl Alcohol  7%
Process of Preparation:
Step 1: 15.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 28 mL of amyl acetate while stirring at room temperature for 30 minutes or until the nitrocellulose dissolves completely in amyl acetate; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: camphor (1.0 g), tea tree oil (1.0 g), ethyl acetate (28.0 g), Acyclovir (4.8 g), ethyl alcohol (15.0 g), isopropyl alcohol (7.0 g), and Benzalkonium (0.2 g) at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 60 minutes or until all the ingredients dissolve completely.

Example 14

(38) (The liquid discreet patch with Magnesium 10%)

(39) TABLE-US-00014 Components Quantity (% w/w) Magnesium 10% Tea Tree Oil  1% Nitrocellulose 10% Camphor  1% Amyl Acetate 19% Ethyl Acetate 19% Ethyl Alcohol 10% Dimethyl Sulfoxide 30%
Process of Preparation:
Step 1: 10.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 30 mL of dimethyl sulfoxide while stirring at room temperature for 60 minutes or until the nitrocellulose dissolves completely in dimethyl sulfoxide; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: L-Magnesium (10.0 g), ethyl alcohol (10.0 g), camphor (1.0 g), tea tree oil (1.0 g), amyl acetate (19.0 g), and ethyl acetate (19.0 g), at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 120 minutes or until all the ingredients dissolve completely.

Example 15

(40) (The liquid discreet patch with Zinc 10%)

(41) TABLE-US-00015 Components Quantity (% w/w) Zinc 10% Tea Tree Oil  1% Nitrocellulose 10% Camphor  1% Amyl Acetate 19% Ethyl Acetate 19% Ethyl Alcohol 10% Dimethyl Sulfoxide 30%
Process of Preparation:
Step 1: 10.0 g of Nitrocellulose (70% dry weight: 30% isopropanol) is mixed with 30 mL of dimethyl sulfoxide while stirring at room temperature for 60 minutes or until the nitrocellulose dissolves completely in dimethyl sulfoxide; mixing speed is 300-1000 rpm
Step 2: Other ingredients are mixed and dissolved separately in the following order: L-Zinc (10.0 g), ethyl alcohol (10.0 g), camphor (1.0 g), tea tree oil (1.0 g), amyl acetate (19.0 g), and ethyl acetate (19.0 g), at room temperature with a mixing speed of 300-1000 rpm. A total mixing time including stage 1) is 120 minutes or until all the ingredients dissolve completely.