LAYERED BLOOD PRODUCT FOR STOPPING/REDUCING BLEEDING IN AN OPEN SURGICAL WOUND

Abstract

A method for stopping or reducing bleeding in open surgical wounds uses blood product that has a first layer, and a second layer. The first layer is adjacent to the second layer and defines a first outer surface of the blood product. The first layer is a majority leukocytes and the second layer is a majority thrombocytes.

Claims

1. A method for stopping or reducing bleeding in an open surgical wound comprising the steps of a. identifying a bleeding source; b. removing excess fluids such as blood from an area around the bleeding source; c. applying a blood product to the bleeding source; and d. maintaining the blood product in close contact with the bleeding source until bleeding is ceased or reduced; wherein the blood product is selected among: i. multilayered blood products comprising components from whole blood, especially thrombocytes and leukocytes, the blood product comprising a first layer, and a second layer, the first layer being adjacent to the second layer, the first layer defining a first outer surface of the blood product, the first layer comprising a majority of leukocytes and the second layer comprising a majority of thrombocytes, and wherein the blood product is oriented so the first layer is in direct contact with the bleeding source; and ii. single layered blood products consisting of components from plasma, components added to initiate a coagulation process and components created during the coagulation process.

2. The method according to claim 1, wherein the blood product further comprises a sheet covering the second layer forming a second surface of the blood product.

3. The method according to claim 2, wherein the sheet consists of a third layer being adjacent to the second layer and defining a second outer surface of the blood product, and wherein the third layer comprises a majority of fibrin.

4. The method according to claim 3, wherein the third layer comprises a further serum component such as thrombin, pro-thrombin and calcium.

5. The method according to claim 1, wherein the thrombocytes is a mixture of thrombocytes or one type defining types that have released their granular, not released their granular.

6. The method according to claim 1, wherein the thrombocytes and/or the leukocytes are autologous.

7. The method according to claim 1, wherein the blood product is dried.

8. A blood product for use in a method for stopping or reducing bleeding in an open surgical wound comprising the steps of a. identifying a bleeding source; b. removing excess fluids from an area around the bleeding source; c. applying a blood product to the bleeding source; and d. maintaining the blood product in close contact with the bleeding source until bleeding is ceased or reduced; wherein the blood product is selected among: i. multilayered blood products comprising components from whole blood, especially thrombocytes and leukocytes, the blood product comprising a first layer, and a second layer, the first layer being adjacent to the second layer, the first layer defining a first outer surface of the blood product , the first layer comprising a majority of leukocytes and the second layer comprising a majority of thrombocytes; and wherein the blood product is oriented so the first layer is in direct contact with the bleeding source; and ii. single layered blood products consisting of components from plasma, components added to initiate a coagulation process and components created during the coagulation process.

9. The blood product according to claim 8, further comprising a sheet covering the second layer forming a second surface of the blood product.

10. The blood product according to claim 9, wherein the sheet consists of a third layer being adjacent to the second layer and defining a second outer surface of the blood product, wherein the third layer comprises a majority of fibrin.

11. The blood product according to claim 10, wherein the third layer comprises a further serum component selected from the group consisting of: thrombin, prothrombin and calcium.

12. The blood product according to claim 8, wherein the thrombocytes is a mixture of thrombocytes or one type defining types that have released their granular, not released their granular.

13. The blood product according to claim 8, wherein the thrombocytes and/or the leukocytes are autologous.

14. The blood product according to claim 8, wherein the blood product is dried.

15. The blood product according to claim 9, wherein the thrombocytes and/or the leukocytes are autologous.

16. The blood product according to claim 10, wherein the thrombocytes and/or the leukocytes are autologous.

17. The blood product according to claim 11, wherein the thrombocytes and/or the leukocytes are autologous.

18. The method according to claim 2, wherein the thrombocytes and/or the leukocytes are autologous.

19. The method according to claim 3, wherein the thrombocytes and/or the leukocytes are autologous.

20. The method according to claim 4, wherein the thrombocytes and/or the leukocytes are autologous.

Description

BRIEF DESCRIPTION OF THE FIGURES

[0086] FIG. 1 shows a scientist displaying the single layer patch prepared in Example 1.

[0087] FIG. 2 shows a picture of a surgery on a pig, showing a Patch placed on bleeding lesion created on the liver and the bleeding was stopped after applying the patch.

EXAMPLES

Example 1—Preparation of Single Layer Patch

[0088] A frozen plasma bag (450 ml) containing human plasma was thawed and calcium chloride was added to initiate coagulation.

[0089] After coagulation was completed, determined by visual inspection, the outlet tube was opened in one end and the whole plasma bag was compressed, by placing a flat surface with a heavy weight on the bag.

[0090] After compression was complete and the bag was flat, the bag was opened, and the single layer patch removed from the bag.

[0091] The generated single layer patch is illustrated on FIG. 1.

Example 2

[0092] For this example, blood products in form of circular patches were prepared using the method described in example 5 in U.S. Pat. No. 8,980,301B, except that Copolyester (PET) containers were used instead of polyamide containers.

[0093] A laboratory pig was used for this example. The pig was anesthetized, and an incision made in the abdomen, so the liver was exposed.

[0094] Cuts were made in the liver leading to bleeding from the wounds.

[0095] Patches were placed covering the bleeding lesions on the liver which led to a fast cessation of the bleeding, whereafter the surgical procedure could swiftly be resumed.

[0096] FIG. 2 shows the surgical incision with the patches covering the lesions.

[0097] In a control experiment where no patches were placed over the bleeding lesions, bleeding continued and thereby providing a challenge for the continued surgical procedure.