FERTILIZERS FOR DELAYING DECAY OF HUANGLONGBING-AFFECTED TREE AND PREPARATION METHODS THEREOF

Abstract

One or more embodiments of the present disclosure provide a special water-soluble fertilizer for delaying decay of a Huanglongbing (HLB)-affected tree. The raw materials of the special water-soluble fertilizer may include, in parts by weight, 3-5.5 parts of ammonium polyphosphate, 7.0-12 parts of ammonium nitrate phosphorus, 13-22 parts of potassium nitrate, 7.5-13.5 parts of ammonium sulfate, 0.8-1.5 parts of urea, 10.5-18 parts of EDTA chelated calcium, 12-21 parts of calcium magnesium nitrate, 3.5-7 parts of citric acid and 150-400 parts of straw vinegar; and a solid content of the straw vinegar may be greater than or equal to 15%. The raw materials may further include 1.3-2.5 parts of EDTA chelated manganese and 1.5-3 parts of manganese sulfate monohydrate. The raw materials may further include 0.25-0.5 part of disodium tetraborate pentahydrate.

Claims

1. A special water-soluble fertilizer for delaying decay of a Huanglongbing (HLB)-affected tree, raw materials of the special water-soluble fertilizer including, in parts by weight, 3-5.5 parts of ammonium polyphosphate, 7.0-12 parts of ammonium nitrate phosphate, 13-22 parts of potassium nitrate, 7.5-13.5 parts of ammonium sulfate, 0.8-1.5 parts of urea, 10.5-18 parts of ethylene diamine tetraacetic acid (EDTA) chelated calcium, 12-21 parts of calcium magnesium nitrate, 3.5-7 parts of citric acid, and 150-400 parts of straw vinegar; a solid content of the straw vinegar is greater than or equal to 15%; the raw materials further include 1.3-2.5 parts of EDTA chelated manganese, 1.5-3 parts of manganese sulfate monohydrate, and 0.25-0.5 part of disodium tetraborate pentahydrate.

2. The special water-soluble fertilizer of claim 1, wherein the straw vinegar is rice straw vinegar, wheat straw vinegar, corn straw vinegar, or rice husk vinegar.

3. The special water-soluble fertilizer of claim 1, wherein the raw materials further include 0.2-0.4 part of EDTA chelated copper, 6-10.5 part of EDTA chelated iron, 0.01-0.02 part of ammonium molybdate heptahydrate, 3.5-6 parts of EDTA chelated zinc, 0.5-2 parts of triclocarban, and 2-3.5 parts of a solubilizer.

4. The special water-soluble fertilizer of claim 3, wherein the solubilizer is alcohol ethoxylates (AEO), polyethylene glycol, or alkylphenol ethoxylates.

5. A preparation method of a special water-soluble fertilizer, comprising: (i) dissolving the ammonium polyphosphate, ammonium nitrate phosphate, potassium nitrate, ammonium sulfate, urea, ethylene diamine tetraacetic acid (EDTA) chelated calcium, calcium magnesium nitrate, citric acid, disodium tetraborate pentahydrate, EDTA chelated copper, EDTA chelated iron, EDTA chelated manganese, EDTA chelated zinc, manganese sulfate monohydrate, and ammonium molybdate in the straw vinegar to obtain a first solution; and (ii) dissolving the triclocarban in the solubilizer to obtain a second solution, dissolving the second solution in the first solution obtained in step (i) under a condition of stirring to obtain a third solution, and filtering the third solution to obtain the special water-soluble fertilizer.

Description

DETAILED DESCRIPTION

[0036] As used in the disclosure and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise. Generally speaking, the terms “comprise,” “comprising”, “comprises”, “including”, “includes”, and “include,” when used in this present disclosure, specify the presence of stated steps and elements, these steps and elements do not constitute an exclusive list, and the method or the device may also include other steps or elements.

[0037] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those having ordinary skills in the art to which the present disclosure belongs.

[0038] One or more embodiments of the present disclosure provide a special fertilizer for delaying decay of a Huanglongbing (HLB)-affected tree.

[0039] In some embodiments, the special fertilizer for delaying the decay of the HLB-affected tree may be a water-soluble fertilizer, of which the raw materials may include, in parts by weight, 3-5.5 parts of ammonium polyphosphate, 7.0-12 parts of ammonium nitrate, 13-22 parts of potassium nitrate, 7.5-13.5 parts of ammonium sulfate, 0.8-1.5 parts of urea, 10.5-18 parts of EDTA chelated calcium, 12-21 parts of calcium magnesium nitrate, 3.5-7 parts of citric acid, and 150-400 parts of straw vinegar.

[0040] In some embodiments, a solid content of the straw vinegar may be greater than or equal to 15%.

[0041] In some embodiments, the water-soluble fertilizer may further include 1.3-2.5 parts of EDTA chelated manganese, and 1.5-3 parts of manganese sulfate monohydrate.

[0042] In some embodiments, the water-soluble fertilizer may further include 0.25-0.5 part of disodium tetraborate pentahydrate (Granubor®).

[0043] In some embodiments, the water-soluble fertilizer may be applied according to a process including: applying the fertilizer to the HLB-affected tree with a dose of 0.5-3 kg/plant once every 15-20 days from January every year, ; wherein the fertilizer may be applied to the HLB-affected tree at least 8 times before a fruit enlargement period of the HLB-affected tree.

[0044] In some embodiments, the special fertilizer for delaying the decay of the HLB-affected tree may be a granular fertilizer. The raw materials of the granular fertilizer may include, in parts by weight, 4.58-6.86 parts of diammonium phosphate, 7.4-11 parts of ammonium phosphorus nitrate, 14-21 parts of potassium nitrate, 7.5-11.5 parts of ammonium sulfate, 0.9-1.3 parts of urea, 4.5-7 parts of calcium carbonate, 20-32 parts of calcium magnesium nitrate, 2.5-4.5 parts of manganese sulfate, and 0.35-0.7 part of borax.

[0045] In some embodiments, the raw materials of the granular fertilizer may further include, in parts by weight, 0.12-0.22 part of copper sulfate, 3.5-6.5 parts of ferrous sulfate, 1.3-2.5 parts of chelated iron, 0.01-0.02 part of ammonium molybdate, and 0.01-0.02 parts of zinc sulfate.

[0046] In some embodiments, the granular fertilizer may be applied according to a process including: applying the granular fertilizer to the citrus HLB-affected tree for three times, applying the granular fertilizer as a base fertilizer to the HLB-affected tree with a dose of 1-2 kg/plant in the first application; applying the granular fertilizer to the HLB-affected tree with a dose of 0.5-1.5 kg/plant during an early flowering period in the second application; and applying the granular fertilizer to the HLB-affected tree with a dose of 1.5-2.5 kg/plant during a fruit enlargement growth period in the third application.

[0047] In some embodiments, the special fertilizer for delaying the decay of the HLB-affected tree body may include a water-soluble fertilizer and a granular fertilizer.

[0048] In some embodiments, the water-soluble fertilizer and the granular fertilizer may be selectively applied during different growth stages of the tree according to actual use requirements. An exemplary method for applying the fertilizer may include: applying the granular fertilizer as a base fertilizer to a citrus HLB-affected tree at least once from November to February every year with an standard does of 1-2 kg/plant; and applying the water-soluble fertilizer as a topdressing fertilizer to the citrus HLB-affected tree with a standard does of 0.5-3 kg/plant once every 20-25 days from March to October every year; and applying the water-soluble fertilizer at least 7 times before a fruit enlargement period. In some embodiments of the present disclosure, the combined application of the base fertilizer and the topdressing fertilizer can make the fruit trees stronger and further delay the decay of the HLB-affected tree bodies.

[0049] In some embodiments, the special fertilizer for delaying the decay of the HLB-affected tree may be a slow release granular fertilizer. In some embodiments, the raw materials of the slow controlled release granular fertilizer may include, in parts by weight, 5.6-8 parts of coated diammonium phosphate, 7.5-11.8 parts of granular ammonium nitrate, 15-24 parts of coated potassium nitrate, 8.5-13.5 parts of coated ammonium sulfate, 1-1.65 parts of coated urea, 5.05-8.5 parts of granular calcium carbonate, 22.5-37.5 parts of coated calcium magnesium nitrate, 2.8-4 parts coated manganese sulfate, 0.4-0.7 part of granular water-soluble manganese sulfate, and 0.41-0.68 part of coated borax.

[0050] In some embodiments, the raw materials of the slow controlled release granular fertilizer may further include 0.15-0.25 part of granular copper sulfate, 0.5-0.83 parts of granular water-soluble ferrous sulfate, 1.5-2.5 parts of granular chelated iron, 3.5-5.8 parts of coated ferrous iron, 0.01-0.02 part of granular ammonium molybdate, 0.18-0.35 part of granular water-soluble zinc sulfate, and 3.1-5.1 parts of coated zinc sulfate.

[0051] In some embodiments, an application method of the slow controlled release granular fertilizer may include: applying the slow controlled release granular fertilizer to the citrus HLB-affected tree only once as a base fertilizer with an application standard of 1-4 kg/plant.

[0052] In some embodiments, the special fertilizer for delaying the decay of the HLB-affected tree may include a water-soluble fertilizer and a slow release granular fertilizer.

[0053] In some embodiments, the water-soluble fertilizer and the slow release granular fertilizer may be selectively applied at different growth stages of the tree according to actual use requirements. An exemplary fertilization method may include: applying the slow release granular fertilizer as a base fertilizer to the citrus HLB-affected tree with a dose of 0.5-2 kg/plant once from November to February every year; and applying the water-soluble fertilizer as a topdressing fertilizer to the citrus HLB-affected tree with a dose of 0.5-3 kg/plant once every 25-30 days from March to October every year, wherein the water-soluble fertilizer may be applied at least 8 times before a fruit enlargement period of the HLB-affected tree. In some embodiments of the present disclosure, the combined application of the base fertilizer and the topdressing fertilizer may be used to further delay the decay of the HLB-affected tree, and the effective components in the fertilizer may release slowly and continuously by coating components of the granular fertilizer, avoiding the waste of the fertilizer components, and improving the use efficiency of the fertilizer.

[0054] The conception of the present disclosure and the technical effects produced will be clearly and completely described hereinafter in conjunction with the embodiments, to fully understand the purpose, scheme and effect of the present disclosure.

EXAMPLES

Example 1

[0055] A slow controlled release fertilizer was prepared, of which the raw materials included, in parts by weight, 6.58 parts of coated diammonium phosphate, 9.22 parts of granular ammonium phosphorus nitrate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 0.62 part of coated manganese sulfate monohydrate, and 0.16 part of granular manganese sulfate monohydrate.

Example 2

[0056] A slow controlled release fertilizer was prepared, of which the raw material included, in parts by weight, 14.12 parts of coated diammonium phosphate, 8.77 parts of granular ammonium phosphorus nitrate, 30 parts of coated potassium nitrate, 12.83 parts of coated ammonium sulfate, 10 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 3.1 parts of coated manganese sulfate monohydrate, and 0.5 part of granular manganese sulfate monohydrate.

Example 3

[0057] A slow controlled release fertilizer was prepared, of which the raw materials included, in parts by weight, 6.58 parts of coated diammonium phosphate, 9.22 parts of granular ammonium phosphorus nitrate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 3.1 parts of coated manganese sulfate monohydrate, and 0.5 part of granular manganese sulfate monohydrate.

Example 4

[0058] A slow controlled release fertilizer was prepared, of which the raw materials included, in parts by weight, 6.58 parts of coated diammonium phosphate, 9.22 parts of granular ammonium phosphorus nitrate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, and 0.52 part of coated sodium borate.

Example 5

[0059] A slow controlled release fertilizer was prepared, of which the raw materials included, in parts by weight, 6.58 parts of coated diammonium phosphate, 9.22 parts of granular ammonium phosphorus nitrate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 0.52 part of coated sodium borate, 3.1 parts of coated manganese sulfate monohydrate, and 0.5 part of granular manganese sulfate monohydrate.

Example 6

[0060] A slow controlled release fertilizer was prepared, of which the raw materials included, in parts by weight, 6.58 parts of coated diammonium phosphate, 9.22 parts of granular ammonium phosphorus nitrate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 0.52 part of coated sodium borate, 3.1 parts of coated manganese sulfate monohydrate, 0.5 part of granular manganese sulfate monohydrate, 0.55 part of granular ferrous sulfate heptahydrate, 1.69 parts of EDTA chelated iron, 4.43 parts of coated ferrous sulfate heptahydrate, 0.16 part of copper sulfate pentahydrate, 0.01 part of ammonium heptamolybdate, 0.23 part of granular zinc sulfate heptahydrate, and 3.9 parts of coated zinc sulfate heptahydrate.

Example 7

[0061] A control group was set. A slow controlled release fertilizer was prepared, of which the raw materials included, in parts by weight, 14.12 parts of coated diammonium phosphate, 8.77 parts of granular ammonium phosphorus nitrate, 30 parts of coated potassium nitrate, 12.83 parts of coated ammonium sulfate, 10 parts of coated urea, 5.63 parts of granular calcium carbonate, and 28.75 parts of coated calcium magnesium nitrate (these are the conventional amount of nitrogen fertilizer, phosphorus fertilizer, potash fertilizer, calcium fertilizer and magnesium fertilizer in the slow controlled release fertilizer for citrus, and may also be the general level of amount used in the prior art.).

Example 8 Comparison Test of Slow Controlled Release Fertilizers Prepared in Examples 1-6 and Example 7

[0062] Contrast tests were carried out on the slow controlled release fertilizers prepared in Examples 1-6 and Example 7. The tests was performed for 3 years.

[0063] Test location: Indoor of Ganzhou Citrus Science Research Institute.

[0064] Test variety: Newhall navel orange. Age of tree: 4 years. All the trees used in the tests are HLB-infected navel orange trees.

[0065] In the test, each treatment was repeated 3 times. There are 21 plots in total, with 5 navel orange trees in each plot. The plots are arranged randomly, and the first tree and the last tree in each plot are marked accordingly.

[0066] Time and method for applying the fertilizer: when applying a base fertilizer every year, spreading the slow controlled release fertilizer evenly on the ground along a drip line, and then plowing 10-20 cm to mix the fertilizer and soil evenly.

[0067] Sampling method: selecting three trees with uniform growth from each plot for yield measurement sampling during a maturity stage every year, each tree being counted separately; collecting five navel orange fruits of uniform size from four different orientations of each tree and mixing into one sample to determine quality indexes and Ct values; mixing four root samples from different orientations of each tree into one sample to determine root morphological indexes and Ct values; and mixing twelve diseased leaves from four different orientations of each tree into one sample to determine Ct values of the midveins of the leaves.

[0068] The Ct values were measured by real-time fluorescent quantitative PCR. The leaf, root and fruit samples were measured by real-time fluorescent quantitative PCR after DNA extraction. Primers used in real-time fluorescent quantitative PCR were HLBasf and HLBr, and a probe may be HLBp. DdH.sub.2O and DNA extracted from healthy navel orange materials were used as a blank control and a negative control, respectively, and the DNA of infected navel oranges were used as a positive control. A 20 .Math.L fluorescent quantitative PCR amplification system contained: 10 .Math.L Bestar qPCR Master Mix, 8 .Math.L ddH2O, 0.4 .Math.L HLBasf (10 .Math.mol/L), 0.4 .Math.L HLBr (10 .Math.mol/L), 0.2 .Math.L HLBp (5 .Math.mol/L) and 1 .Math.L DNA solution. A Real-Time PCR instrument was used for PCR. Reaction conditions were: 95° C., 2 min, 95° C., 10 s; 60° C., 30 s, 40 cycles.

[0069] A root morphology was measured using a root scanner LA2400 and analyzed by a WinRHIZO analysis system. The fertilization conditions are shown in Table 1.

TABLE-US-00001 Example Usage content Amount of each fertilization (kg/plant) Example 7 (Control group) Normal amount of nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, calcium fertilizer and magnesium fertilizer 2 Example 1 Reduction of nitrogen fertilizer by 33%, phosphorus fertilizer by 50%, and potassium fertilizer by 33%; Normal amount of calcium fertilizer, magnesium fertilizer and manganese fertilizer 2 Example 2 Normal amount of nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, calcium fertilizer and magnesium fertilizer; increase of manganese fertilizer by 300% 2 Example 3 Reduction of nitrogen fertilizer by 33%, phosphorus fertilizer by 50%, potassium fertilizer by 33%; normal amount of calcium fertilizer, and magnesium fertilizer, and increase of manganese fertilizer by 300% 2 Example 4 Reduction of nitrogen fertilizer by 33%, phosphorus fertilizer by 50%, and potassium fertilizer by 33%; normal amount of calcium fertilizer, and magnesium fertilizer; and adding borax fertilizer to soil (borax is usually used on foliar) 2 Example 5 Reduction of nitrogen fertilizer by 33%, phosphorus fertilizer by 50%, and potassium fertilizer by 33%; normal amount of calcium fertilizer, and magnesium fertilizer; an increase of manganese fertilizer by 300% and applying borax fertilizer to the soil. 2 Example 6 Reduction of nitrogen fertilizer by 33%, phosphorus fertilizer by 50%, and potassium fertilizer by 33%; normal amount of calcium fertilizer and magnesium fertilizer; an increase of manganese fertilizer by 300%; adding iron, zinc, copper, molybdenum fertilizer and other trace elements, and applying borax fertilizer to the soil. 2

[0070] Table 2 shows the effect of granular fertilizers on the yield of navel oranges under different fertilizers.

TABLE-US-00002 Example First year (kg/plant) Second year (kg/plant) Third year (kg/plant) Example 7 (Control group) 36.45 35.04 31.97 Example 1 34.56 32.14 29.55 Example 2 35.12 33.22 44.19 Example 3 32.67 39.50 49.15 Example 4 35.01 36.03 46.85 Example 5 36.09 45.48 55.95 Example 6 37.18 47.21 61.06

[0071] Table 3 shows the effect of different granular fertilizers on the quality of navel oranges.

TABLE-US-00003 Example Total sugar (%) Soluble solid (%) Titratable acid (%) Vc (mg/100 g) Edible rate (%) Juice rate (%) Solidity-acid ratio Example 7 (Control group) 5.68 8.20 0.45 37.50 52.50 31.40 18.21 Example 1 5.31 8.77 0.51 35.40 50.20 30.50 17.20 Example 2 8.66 10.91 0.59 45.70 70.60 41.70 18.49 Example 3 9.72 12.16 0.57 46.50 72.70 43.60 21.34 Example 4 9.16 10.78 0.59 44.20 68.70 40.50 18.27 Example 5 10.24 13.70 0.60 50.80 73.10 47.90 22.84 Example 6 10.71 14.62 0.61 54.40 75.20 49.50 23.96

[0072] Table 4 shows the effect of different granular fertilizers on the root system of navel oranges.

TABLE-US-00004 Example Total length of root (cm) Total area of root surface (cm.sup.2) Total volume of root (cm.sup.3) Average root diameter (mm) Number of root tips Example 7 (Control group) 4556.35 1035.68 20.57 0.54 5238.17 Example 1 4687.71 1117.94 22.09 0.58 5363.34 Example 2 7874.87 1483.64 30.56 0.82 8778.95 Example 3 8142.52 1590.97 31.73 0.82 9050.81 Example 4 7633.36 1410.30 28.80 0.81 8562.09 Example 5 10021.74 1919.90 39.97 0.85 10930.74 Example 6 11259.31 2001.68 38.48 0.82 11154.45

[0073] Table 5 shows the effects of different granular fertilizers on the HLB pathogen bacteria content (Ct value) in navel orange roots, leaves and fruits.

TABLE-US-00005 Example Number of samples (N) First year Second year Third year Root system Midveins of leaves Fruits Root system Midveins of leaves Fruits Root system Midveins of leaves Fruits Example 7 (Control group) 9.00 25.32 23.23 24.09 27.29 24.91 25.14 23.67 21.93 21.42 Example 1 9.00 24.66 22.75 26.63 28.44 26.73 27.53 27.54 24.66 25.77 Example 2 9.00 25.67 25.44 24.17 31.85 28.88 28.93 35.15 33.44 32.33 Example 3 9.00 26.15 23.21 23.93 33.73 30.27 29.89 35.77 34.12 33.94 Example 4 9.00 24.28 24.12 24.22 29.61 32.25 30.68 33.20 35.05 35.35 Example 5 9.00 26.79 25.10 25.01 35.40 33.50 32.71 36.03 35.16 35.54 Example 6 9.00 26.88 24.79 25.66 35.66 34.67 34.19 35.96 36.85 36.65

[0074] Note: The test result was displayed by the number of cycles experienced when a fluorescent signal in a reaction tube reaches a set threshold (i.e., the Ct value). A Ct value less than 35 indicates that the sample is positive for HLB, and a Ct value greater than 35 indicates that the sample is negative for HLB. The lower the Ct value is, the higher the content of HLB pathogenic bacteria is.

[0075] The following conclusions can be found from the data in Tables 2-5: (1) excessive application of manganese fertilizer can inhibit the number of HLB pathogenic bacteria in the root system of citrus; while the effect of normal amount of manganese fertilizer supplementation in Example 1 is not significant; (2) when nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer are reduced by 30%-50%, a 300% increase of manganese fertilizer can better inhibit the number of HLB pathogenic bacteria in the root system; (3) a combined application of manganese fertilizer and boron fertilizer is much better than single application in inhibiting the content of citrus HLB pathogenic bacteria; and (4) in the case of 30%-50% reduction of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer, combined application of incremental boron fertilizer, manganese fertilizer and other trace elements can significantly restore the tree vigor of HLB-affected trees in the second year, and achieve high yield and high quality.

Example 9

[0076] A special water-soluble fertilizer was prepared, of which the raw material included, in parts by weight, 4.37 parts of ammonium polyphosphate, 9.4 parts of ammonium phosphorus nitrate, 17.4 parts of potassium nitrate, 10.5 parts of ammonium sulfate, 1.1 parts of urea, 14.5 parts of EDTA chelated calcium, 16.5 parts of calcium magnesium nitrate, 5 parts of citric acid, and 250 parts of straw vinegar concentrate (21.14% solid content).

Example 10

[0077] A special water-soluble fertilizer was prepared, of which the raw materials included, in parts by weight, 4.37 parts of ammonium polyphosphate, 9.4 parts of ammonium phosphorus nitrate, 17.4 parts of potassium nitrate, 10.5 parts of ammonium sulfate, 1.1 parts of urea, 14.5 parts of EDTA chelated calcium, 16.5 parts of calcium magnesium nitrate, 5 parts of citric acid, 0.35 part of Granubor®, and 250 parts of straw vinegar concentrate (21.14% solid content).

Example 11

[0078] A special water-soluble fertilizer was prepared, of which the raw materials may include, in parts by weight, 4.37 parts of ammonium polyphosphate, 9.4 parts of ammonium phosphorus nitrate, 17.4 parts of potassium nitrate, 10.5 parts of ammonium sulfate, 1.1 parts of urea, 14.5 parts of EDTA chelated calcium, 16.5 parts of calcium magnesium nitrate, 5 parts of citric acid, 1.77 parts of EDTA chelated manganese, 2.15 parts of manganese sulfate monohydrate, and 250 parts of straw vinegar concentrate (21.14% solid content).

Example 12

[0079] A special water-soluble fertilizer was prepared, of which the raw material may include, in parts by weight, 4.37 parts of ammonium polyphosphate, 9.4 parts of ammonium phosphorus nitrate, 17.4 parts of potassium nitrate, 10.5 parts of ammonium sulfate, 1.1 parts of urea, 14.5 parts of EDTA chelated calcium, 16.5 parts of calcium magnesium nitrate, 5 parts of citric acid, 1.77 parts of EDTA chelated manganese, 0.35 parts of Granubor®, 2.15 parts of manganese sulfate monohydrate, and 250 parts of straw vinegar concentrate (21.14% solid content).

Example 13

[0080] A special water-soluble fertilizer was prepared, of which the raw materials included, in parts by weight, 4.37 parts of ammonium polyphosphate, 9.4 parts of ammonium phosphorus nitrate, 17.4 parts of potassium nitrate, 10.5 parts of ammonium sulfate, 1.1 parts of urea, 14.5 parts of EDTA chelated calcium, 16.5 parts of calcium magnesium nitrate, 5 parts of citric acid, 0.35 part of Granubor®, 0.27 part of DTA chelated copper, 8.5 parts of DTA chelated iron, 1.77 parts of DTA chelated manganese, 4.8 parts of DTA chelated zinc, 2.15 parts of manganese sulfate monohydrate, 0.01 part of ammonium molybdate, 0.7 part of triclocarban, 2.8 parts of polyethylene glycol, and 250 parts of straw vinegar concentrate (21.14% solid content).

Example 14

[0081] A control group (CK) was set. A water-soluble fertilizer was prepared, of which the raw materials included, in parts by weight, 4.37 parts of ammonium polyphosphate, 9.4 parts of ammonium phosphorus nitrate, 17.4 parts of potassium nitrate, 10.5 parts of ammonium sulfate, 1.1 parts of urea, 14.5 parts of EDTA chelated calcium, 16.5 parts of calcium magnesium nitrate, 5 parts of citric acid, 250 parts of straw vinegar concentrate (7.86% solid content).

Example 15 Compassion Test of Water-Soluble Fertilizer in Examples 9-13 and Example 14

[0082] Contrast tests were carried out on the water-soluble fertilizers in Examples 9-13 and Example 14.

[0083] Test location: the self-made net of Fan’s orchard in Jiaolin Village, Jiangkou Town, Ganxian District, Ganzhou City. The tests are continuously performed for 3 years at the location.

[0084] Test variety: Newhall navel orange. Age of tree: 5 years. All the trees used in the tests were HLB-infected.

[0085] During the test, each treatment may be repeated 3 times. There were18 plots in total, with 6 navel orange trees in each plot. The plots were arranged in random blocks, and the first tree and the last tree in each plot were marked accordingly.

[0086] Time and method for applying the fertilizers: applying the fertilizer to the HLB-affected tree with a dose of 1.5 kg/plant once every 20 days from January every year; wherein the fertilizers were applied 11 times before the fruit enlargement period of the HLB-affected tree.

[0087] Sampling method: selecting four tree bodies with uniform growth from each plot for yield measurement sampling during a navel orange maturity stage every year, each tree being counted separately; collecting five navel orange fruits of uniform size from four different orientations of each tree and mixing into one sample to determine quality indexes and Ct values; mixing four root samples from different orientations of each tree into one sample to determine root morphological indexes and Ct values; and mixing twelve diseased leaves from four different orientations of each tree into one sample to determine Ct values of the midveins of the leaves.

[0088] The Ct values were measured by real-time fluorescent quantitative PCR, and the leaf, root and fruit samples were measured by real-time fluorescent quantitative PCR after DNA extraction. Primers used in real-time fluorescent quantitative PCR were HLBasf and HLBr, and a probewas HLBp. ddH2O and DNA extracted from healthy navel orange materials were used as a blank control and a negative control, respectively, and the DNA of navel oranges known to be infected was used as a positive control. A 20 .Math.L fluorescent quantitative PCR amplification system contained: 10 .Math.L Bestar qPCR Master Mix, 8 .Math.L ddH2O, 0.4 .Math.L HLBasf (10 .Math.mol/L), 0.4 .Math.L HLBr (10 .Math.mol/L), 0.2 .Math.L HLBp (5 .Math.mol/L) and 1 .Math.L DNA solution. PCR was performed using a Real-Time PCR instrument. The reaction conditions were: 95° C., 2 min, 95° C., 10 s; 60° C., 30 s, 40 cycles.

[0089] The root morphology was measured by a root scanner LA2400 and analyzed by a WinRHIZ0 analysis system. The fertilizer application details of the water-soluble fertilizer in each group is shown in Table 6.

TABLE-US-00006 Example No. Purpose Amount of each fertilization (kg/plant) Example 14 (Control group) Investigating the effect of the straw vinegar with the solid content less than or equal to 15% based on nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, calcium fertilizer and magnesium fertilizer 1.5 Example 9 Investigating the effect of the straw vinegar with the solid content greater than or equal to 15% based on nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, calcium fertilizer and magnesium fertilizer 1.5 Example 10 Investigating the interaction effect of the straw vinegar with the solid content greater than or equal to 15% and boron fertilizer based on nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, calcium fertilizer and magnesium fertilizer 1.5 Example 11 Investigating the interaction effect of the straw vinegar with the solid content greater than or equal to 15% and manganese fertilizer based on nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, calcium fertilizer and magnesium fertilizer 1.5 Example 12 Investigating the interaction effect of the straw vinegar with the solid content greater than or equal to 15%, manganese fertilizer and boron fertilizer based on nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, calcium fertilizer and magnesium fertilizer 1.5 Example 13 Investigating the interaction effect of the straw vinegar with the solid content greater than or equal to 15%, manganese fertilizer, boron fertilizer, zinc fertilizer and other trace elements based on nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, calcium fertilizer and magnesium fertilizer 1.5

[0090] Table 7 shows the effect results of the different water-soluble fertilizers on the yield of navel oranges.

TABLE-US-00007 Example First year(kg/plant) Second year(kg/plant) Thirdyear(kg/plant) Example 14 (Control group) 34.11 35.54 37.47 Example 9 39.40 44.49 51.77 Example 10 30.53 46.40 63.71 Example 11 37.44 63.85 67.35 Example 12 45.22 67.60 69.28 Example 13 60.70 74.76 78.29

[0091] Table 8 shows the effect results of the different water-soluble fertilizers on the quality of navel oranges.

TABLE-US-00008 Example Total sugar(%) Soluble solid (%) Titratable acid (%) Vc(mg/100 g) Edible rate (%) Juice rate (%) Solidity-acid ratio Example 14 (Control group) 7.96 8.50 0.50 41.73 62.60 37.73 17.11 Example 9 8.64 9.50 0.51 44.38 67.21 41.88 18.63 Example 10 9.40 12.50 0.55 48.61 70.73 46.73 22.80 Example 11 10.92 13.00 0.55 49.66 72.86 47.09 23.48 Example 12 11.55 14.50 0.57 52.36 75.31 50.94 25.51 Example 13 12.13 15.00 0.58 54.13 77.68 52.74 25.86

[0092] Table 9 shows the effect results of the different water-soluble fertilizers on the root systems of navel oranges.

TABLE-US-00009 Example Total length of root(cm) Total area of root surface (cm.sup.2) Total volume of root(cm.sup.3) Average root diameter(mm) Number of root tips Example 14 (Control group) 10413.81 2087.53 38.63 0.63 12602.02 Example 9 12672.42 2309.55 41.40 0.80 14160.01 Example 10 16128.06 2527.52 48.77 0.87 17567.69 Example 11 17223.19 2988.51 54.06 0.92 18711.01 Example 12 19663.34 3347.21 66.38 1.04 22258.53 Example 13 20195.12 3525.36 68.97 1.05 23813.70

[0093] Table 10 shows the effect results of the different water-soluble fertilizers on the HLB pathogen bacteria content (Ct value) in navel orange roots, leaves and fruits.

TABLE-US-00010 Example Number of samples (N) First year Second year Third year Root system Midveins of leaves Fruits Root system Midveins of leaves Fruits Root system Midveins of leaves Fruits Example 14 (Control group) 12.00 23.27 20.67 22.32 26.44 23.35 25.16 27.06 26.93 28.11 Example 9 12.00 28.28 26.43 24.25 31.06 30.67 29.50 33.91 32.36 30.30 Example 10 12.00 24.64 25.71 24.51 28.09 31.54 32.15 35.97 35.15 35.11 Example 11 12.00 31.45 29.57 28.46 35.76 35.02 34.45 35.03 36.89 35.13 Example 12 12.00 34.11 32.54 30.17 35.67 35.76 35.17 35.59 35.54 36.28 Example 13 12.00 35.12 35.04 35.07 35.90 35.79 35.48 35.91 36.80 36.76

[0094] Note: The test results were displayed by the number of cycles experienced when a fluorescent signal in a reaction tube reaches a set threshold (i.e., the Ct value). A Ct value less than or equal to 35 indicates that the sample is positive for HLB, and a Ct value greater than 35 indicates that the sample is negative for HLB. The lower the Ct value is, the higher the content of HLB pathogenic bacteria is.

[0095] The following conclusions are found from the data in Tables 7-10: (1) the straw vinegar with the solid content greater than or equal to 15% can reduce the number of HLB pathogenic bacteria in the root system; and the straw vinegar (CK) with the solid content less than 15% has no obvious effect; (2) a combined application of the straw vinegar with the solid content greater than 15% and excessive manganese fertilizer has a better inhibitory effect on the number of citrus HLB pathogenic bacteria than single application. The results of field experiments show that the combined application of the straw vinegar with the solid content greater than or equal to 15% and excessive manganese fertilizer can effectively inhibit the number of HLB pathogenic bacteria in the root system, and HLB pathogenic bacteria in the root system were detected to be negative in the second year of application, obviously restoring the tree vigor, and achieving high yield and high quality; and (3) combined application of the straw vinegar with the solid content greater than or equal to 15% and triclocarban, boron fertilizer, manganese fertilizer and other trace elements can make HLB pathogenic bacteria of the HLB-affected tree negative in the first year, significantly restoring the tree vigor, and achieving high yield and high quality in the same year.

[0096] The above descriptions are only preferred embodiments of the present disclosure, and the present disclosure is not limited to the embodiments, as long as they achieve the technical effects of the present disclosure by the same means, shall fall within the protection scope of the present disclosure. Various modifications and alterations may be made to the technical solutions and/or embodiments within the protection scope of the present disclosure.