NOVEL USE

Abstract

The present invention relates to the use of 1,3-propanediol as an agent to balance the growth of commensal skin microorganisms of dry and sebaceous skin and thus to foster the maintenance of a healthy skin barrier.

Claims

1. A cosmetic, non-therapeutic use of a cosmetic composition comprising 1,3-propanediol in an amount selected in the range from 0.01 to 6.0 wt.-%, based on the total weight of the cosmetic composition for balancing the growth of at least two microbes of dry or sebaceous skin by not unproportionally affecting the growth of anyone thereof and the growth of S. aureus in the presence of S. epidermidis by inhibiting the growth of S. aureus to a higher extent than the one of S. epidermidis.

2. The use according to claim 1, wherein the microbes of dry skin are selected from C. tuberculostearicum, C. acnes, S. mitis, S. oralis, M. luteus and M. globosa and the microbes of sebaceous skin are selected from the group of C. acnes, S. epidermidis, S. hominis, S. capitis, S. mitis, C. simulans and M. globosa.

3. The use according to claim 1, wherein the balancing of the growth of the at least two, preferably all microbes of dry or sebaceous skin is characterised in that the difference in the share of each individual microbe, compared to the respective share in the absence of 1,3-propanediol, after 4 h of incubation at 37° C., is in the range of ±15%, preferably in the range of ±10%, most preferably in the range of ±7.5%.

4. The use according to claim 1, wherein the balancing of the growth of the at least two, preferably all microbes of dry or sebaceous skin is characterised in that the difference in the total number of said at least two, preferably all microbes, compared to the respective total number in the absence of 1,3-propanediol, after 4 h of incubation at 37° C., is within a range of ±50%, preferably ±40%, more preferably ±15%, most preferably ±10%.

5. The use according to claim 2, wherein the difference in total number of each of the at least two, preferably all microbes of dry skin are within the range of ±20%, preferably 0% to 20%, more preferably +5% to +15%, most preferably +7.5% to +15% for C. tuberculostearicum, ±15%, preferably 0% to +15%, more preferably +2.5% to +10%, most preferably +5% to +10% for C. acnes, ±75%, preferably 0% to +75%, more preferably +25% to +75%, most preferably +50% to +75% for S. mitis and S. oralis, ±20%, preferably 0% to 20%, more preferably +5% to +15%, most preferably +7.5% to +15% for M. luteus, and/or ±15%, preferably 0% to +15%, more preferably +2.5% to +10%, most preferably +5% to +10% for M. globosa with respect to the total number of each of the microbes in the absence of 1,3-propanediol.

6. The use according to claim 2, wherein the difference in total number of each of the at least two, preferably all microbes of sebaceous skin, is within the range of ±15%, preferably +5% to -15%, more preferably 0% to -10%, most preferably -2.5% to -7.5% for C. acnes, ±35%, preferably +5% to +35%, more preferably +15% to + 30%, most preferably +20% to +30% for S. epidermidis, ±15%, preferably 0% to +15%, more preferably +2.5% to + 10%, most preferably +2.5% to +7.5% for S. hominis, ±20%, preferably 0% to -20%, more preferably -7.5% to -15%, most preferably -10% to -15% for S. capitis, ±25%, preferably -5% to -25%, more preferably -10% to -23%, most preferably -15% to -20% for S. mitis, ±15%, preferably 0% to -15%, more preferably -2.5% to -10%, most preferably -5% to -10% for C. simulans, and/or ±25%, preferably -5% to -25%, more preferably -10% to -23%, most preferably -15% to -23% for M. globosa with respect to the total number of each of the microbes in the absence of 1,3-propanediol.

7. The use according to claim 1, wherein the balancing of the growth of S. aureus in the presence of S. epidermidis is characterized in that the difference in the share of S. epidermidis, in the presence of 1,3-propanediol, before and after 4 h of incubation at 37° C., is less than -25%, preferably less than -20%, most preferably less than -15%.

8. The use according to claim 1, wherein a healthy skin microbiome is maintained.

9. A method of maintaining a healthy skin microbiome in dry or sebaceous skin, said method comprising the step of topically applying a cosmetic composition comprising 1,3-propanediol in an amount selected in the range from 0.01 to 6.0 wt.-%, based on the total weight of the cosmetic composition.

10. A method according to claim 9, wherein the healthy microbiome is maintained by balancing at least two microbes selected from the group consisting of M. globosa, C. tuberculostearicum, M. luteus, C. acnes, S. mitis, S. oralis, S. hominis, S. capitis, C. simulans and/or S. epidermidis.

11. A method according to claim 9, wherein the healthy microbiome is maintained a. in dry skin by balancing the growth of C. tuberculostearicum, C. acnes, S. mitis, S. oralis, M. luteus and M. globosa, b. in sebaceous skin by balancing the growth of C. acnes, S. epidermidis, S. hominis, S. capitis, S. mitis, C. simulans and M. globosa.

12. 1,3-Propanediol for use in counteracting the weakening of the skin barrier caused by a disbalance of the skin microbiome as well as in preventing or reducing the penetration of exogeneous molecules and/or microorganisms following such a weakening.

13. The use according to claim 12, wherein the exogeneous molecules are irritant, toxic or allergenic substances.

14. The use according to claim 12, wherein the microorganisms are pathogenic microbes, in particular S. aureus.

15. Use of 1,3-propanediol as a prebiotic for S. epidermidis and/or S. hominis, preferably in the concomitant presence of C. acnes, S. capitis, S. mitis, C. simulans and M. globosa and/or S. aureus.

Description

[0099] The following examples are provided to further illustrate the compositions and effects of the present invention. These examples are illustrative only and are not intended to limit the scope of the invention in any way.

MICROBIOME BALANCE MODEL

1. Assay

[0100] 5 mL of TSB (Tryptic Say Broth) to which no (Ref.1) or 500 .Math.L of 2 wt.-% of 1,3-propanediol (1,3-PDO) in PBS (Phosphate-buffered saline) have been added. Then this solutions have been inoculated by 500 .Math.L of the respective calibrated mixture of the microbes as outlined below. Directly after inoculation and/or after 4 hours of incubation at 37° C., 100 .Math.L have been taken and plated on an Agar gel petri dish. The microbes have then been counted based on the colony specific morphology and/or size. The results are presented below.

2. Calibrated Mixture of the Microbes

[0101] TABLE-US-00001 1. S. epidermidis/ S. aureus CFU/ml S. epidermidis 2.3E+03 S. aureus 1.9E+03

TABLE-US-00002 2. Sebaceous skin CFU/ml P. acnes 1.4E+05 S. epidermidis 2.4E+04 S. hominis 2.8E+04 S. capitis 1.1E+04 S. mitis 5.4E+04 C. simulans 4.3E+04 M. globosa 2.2E+04

TABLE-US-00003 3. Dry skin CFU/ml C. tuberculostearicum 2.2E+04 P. acnes 1.4E+05 S. mitis 5.4E+04 S. oralis 5.8E+04 M. luteus 1.7E+04 M. globosa 2.2E+04

Result 1: Microbiome Balancing Between S. Epidermidis and S. Aureus

[0102] Table 1 shows the counts of colony (CFU/ml) of S. epidermidis (CSE0) and S. aureus (CSA0) directly after inoculation and the counts of colony of S. epidermidis (CSE4h) and S. aureus (CSA4h) after 4 hours of incubation. All shares have been rounded.

TABLE-US-00004 CSE0 CSA0 CSE4h CSA4h CFU/ml (share) Control 165 (89%) 20 (11%) 120 (40%) 180 (60%) 1,3-PDO 235 (89%) 30 (11%) 170 (77%) 50 (23%)

[0103] In FIG. 1, the distribution of the microbes directly after inoculation (t = 0) and after 4 h (t = 4 h) are depicted. As can be retrieved from table 1, the share of S. epidermidis is significantly altered by the presence of 1,3-PDO, in favour of S. epidermidis.

Result 2: Microbiome Balancing of Microbes of Sebaceous Skin

[0104] Table 2 shows the counts of colony (CFU/ml) of the respective microbes after 4 hours of incubation @ 37° C. All shares have been rounded.

TABLE-US-00005 Microbe Control 1,3-PDO Results CFU/ml share CFU/ml share Change Valuation C. acnes 2836 24% 2664 25% +1%.sup.1 1.sup.3 S. epidermidis 1800 16% 2255 21% +5%.sup.1 2.sup.3 S. hominis 355 3% 373 3% ±0%.sup.1 1.sup.3 S. capitis 745 6% 664 6% ±0%.sup.1 2.sup.3 S. mitis 1591 14% 1300 12% -2%.sup.1 2.sup.3 C.simulans 682 6% 627 6% ±0%.sup.1 1.sup.3 M. globosa 3600 31% 2900 27% -4%.sup.1 2.sup.3 Total 11609 100% 10783 100% -7%.sup.2 1.6.sup.4 .sup.1[value = (share control) - (share 1,3-PDO)] .sup.2[value = (total CFU/ml 1,3-PDO) - (total CFU/ml control) / (total CFU/ml control)*100%)] .sup.3[impact value (x(i)′] .sup.4[overall impact value y]

Result 3: Microbiome Balancing of Microbes of Dry Skin

[0105] Table 3 shows the counts of colony (CFU/ml) of the respective microbes after 4 hours of incubation @ 37° C. in the absence and in the presence of 1,3-PDO. All shares have been rounded.

TABLE-US-00006 Microbe Control 1,3-PDO Results CFU/ml share CFU/ml share Change Valuation C. tuberculostearicum 818 5% 909 4% -1%.sup.1 1.sup.3 P. acnes 3000 20% 3200 15% -4%.sup.1 1.sup.3 S. mitis 3800 25% 6600 31% +6%.sup.1 3.sup.3 S. oralis 3300 22% 5700 27% +5%.sup.1 3.sup.3 M. luteus 782 5% 873 4% -1%.sup.1 1.sup.3 M. globosa 3600 24% 3800 18% -6%.sup.1 1.sup.3 Total 15300 21082 +38%.sup.2 1.7.sup.4 .sup.1[value = (share control) - (share 1,3-PDO)] .sup.2[value = (total CFU/ml 1,3-PDO) - (total CFU/ml control) / (total CFU/ml control)*100%)] .sup.3[impact value (x(i)′] .sup.4[overall impact value]

Comparative Example

[0106] The experiments as outlined above have been repeated, using pentylene glycol (1,2-pentanediol) instead of 1,3-propanediol.

[0107] Table 4 shows the counts of colony (CFU/ml) of S. epidermidis (CSE0) and S. aureus (CSA0) directly after inoculation and the counts of colony of S. epidermidis (CSE4h) and S. aureus (CSA4h) after 4 hours of incubation with pentylene glycol. All shares have been rounded.

TABLE-US-00007 CSE0 CSA0 CSE4h CSA4h CFU/ml (share) control 900 (77%) 270 (23%) 3900 (54%) 4500 (46%) pentylene glycol 1100 (80%) 280 (20%) 3200 (55%( 3900 (45%(

[0108] As can be retrieved from table 4, the share of S. aureus is not reduced in favour of S. epidermidis by the presence of pentylene glycol.

[0109] Furthermore, the microbiome balancing of microbes of sebaceous skin was assessed in the presence of pentylene glycol.

[0110] Table 5 shows the counts of colony (CFU/ml) of the respective microbes after 4 hours of incubation @ 37° C. All shares have been rounded.

TABLE-US-00008 Microbe Control Pentylene Glycol Results CFU/ml share CFU/ml share Change Valuation C. acnes 3900 39.50% 2000 20% -19.5%.sup.1 3.sup.3 S. epidermidis 1500 15% 3300 32% +17%.sup.1 3.sup.3 S. hominis 1900 19% 2100 21% ±2%.sup.1 2.sup.3 S. capitis 880 9% 1100 11% ±2%.sup.1 3.sup.3 S. mitis 340 3.50% 380 4% +0.5%.sup.1 2.sup.3 C.simulans 650 7% 450 4% -3%.sup.1 2.sup.3 M. globosa 730 7% 830 8% +1%.sup.1 2.sup.3 Total 9900 100% 10160 100% +2.60%.sup.2 2.4.sup.4 .sup.1[value = (share control) - (share Pentylene Glycol)] .sup.2[value = (total CFU/ml Pentylene Glycol) - (total CFU/ml control) / (total CFU/ml control)*100%)] .sup.3[impact value (x(i)′] .sup.4[overall impact value y]

[0111] As can be retrieved from table 5 above, in contrast to 1,3-propanediol, pentylene glycol does alter the share of the microbes of sebaceous skin to a greater extend and can thus not be considered as being particularly microbiome friendly.

FORMULATION EXAMPLE

[0112] TABLE-US-00009 Wipe impregnated 1 INCI name % w / w % w/w range Aqua Ad 100 Add 100 Disodium EDTA 0.20 0.1-0.5 Aqua and Saccharide isomerate and citric acid and sodium citrate 1.00 0.1-3.00 1,3 Propanediol - TILAMAR PDO 2.00 0.5-5.00 PEG-6 Caprylic/Capric glycerides 2.00 1.00-3.00 Microcrystalline Cellulose and Cellulose gum 0.50 0.1-1.00 Citrci acid 0.20 0.05-0.50 Aqua and sodium benzoate and potassium sorbate 1.50 0.30-2.00 Tocpherol 0.10 0.05-1.00

TABLE-US-00010 Wipe impregnated 2 INCI name % w / w % w/w range Aqua Add 100 Add 100 1,3 Propanediol - TILAMAR PDO 3.00 0.5-5.00 Sodium gluconate 0.10 0.05-0.50 Aqua and and Glycerin and Levulinic acid and sodium levulinate 1.00 0.50.5.00 Sodium benzoate 0.25 0.05-0.50 Aqua and Glycerin and Epilobium fleischeri flower/leaf/stem extract and citric acid 0.50 0.1-1.50 Caprylyl/Capryl Glucoside 1.50 0.80-2.00 Aqua and Sodium hydroxide (10% solution) 0.50 0.20-0.80