Compositions and methods for controlling a honey bee parasitic mite infestation

Abstract

The present invention relates to a formula or composition for use in reducing a honey bee parasitic mite infestation that may comprise, for example, a liquid, solid, or paste composition, comprising about 5-20% beta acids (about 5-75% by weight), about 5-75% by weight propylene glycol, about 5-75% by weight polysorbate 60, about 0.5-35% of a thixotropic material comprising, for example, fumed silica, and/or about 0.5-5% of an antioxidant comprising, for example, ascorbic acid, the composition is active against parasitic mites for more than about 14 days in the bee hives. Compositions of the present invention provide effective control, treatment, or prevention of honey bee parasitic mite infestation by inclusion of, for example, certain excipients that retard beta acid oxidation, prolong availability of active ingredient for bee uptake, improve convenience for beekeepers, or both retard beta acid oxidation and prolong the availability of active ingredient for bee uptake.

Claims

1. A composition comprising about 5% to about 75% by weight hop beta acids, about 5% to about 29% by weight solvent, about 5% to about 75% by weight emulsifier, and about 0.5% to about 35% by weight fumed silica, wherein the hop beta acid degradation is about 30% over a period of about 30 days, and wherein the composition is moist for a period of at least about 14 days.

2. The composition of claim 1, wherein at least one of the hop beta acids are potassium salts of hop beta acids; the solvent is propylene glycol; and the emulsifier is polysorbate 60.

3. The composition of claim 1, wherein the composition comprises about 30% to about 35% by weight hop beta acids, about 10% to about 29% by weight solvent, about 30% to about 35% by weight emulsifier, about 0.5% to about 25% by weight fumed silica and, optionally, further comprising about 5% by weight ascorbic acid.

4. The composition of claim 1, wherein the composition is in the form of a liquid, a strip, a patty, a paste, a pad, or a powder.

5. The composition of claim 1, wherein the fumed silica maintains composition availability for bee uptake or reduces beta acid degradation or reduces bee agitation by minimum dripping on bees in beehives.

6. The composition of claim 1, wherein the composition further comprises 0.5% to about 5% by weight ascorbic acid or another antioxidant.

7. The composition of claim 6, wherein the ascorbic acid reduces beta acid degradation.

8. The composition of claim 6, comprising both fumed silica and ascorbic acid.

9. The composition of claim 1, wherein the composition is active against parasitic mites.

10. A method of controlling a honey bee parasitic mite infestation in a honey bee population comprising: exposing a honey bee population to the composition of claim 1.

11. The method of claim 10, wherein at least one of the hop beta acids are potassium salts of hop beta acids; the solvent is propylene glycol; and the emulsifier is polysorbate 60.

12. The method of claim 10, further comprising about 20% to about 35% by weight hop beta acids, about 20% to about 29% by weight solvent, about 20% to about 35% by weight emulsifier, and 15% by weight fumed silica and, optionally, further comprising about 5% by weight ascorbic acid.

13. The method of claim 10, further comprising providing the composition in the form of a strip, a patty, a paste, a pad, or a powder.

14. The method of claim 10, further comprising 0.5% to about 5% by weight ascorbic acid.

15. The method of claim 10, further comprising providing ascorbic acid in an amount sufficient to reduce beta acid degradation.

16. The method of claim 12, comprising both fumed silica and ascorbic acid.

17. The composition of claim 1, wherein the composition is in the form of a liquid, a patty, a paste, or a pad.

18. A composition comprising about 5% to about 75% by weight hop beta acids, about 5% to about 75% by weight solvent, about 5% to about 75% by weight emulsifier, and about 0.5% to about 6% by weight fumed silica, wherein the hop beta acid degradation is about 30% over a period of about 30 days, and wherein the composition is moist for a period of at least about 14 days.

19. The composition of claim 18, wherein at least one of the hop beta acids are potassium salts of hop beta acids; the solvent is propylene glycol; and the emulsifier is polysorbate 60.

20. The composition of claim 18, wherein the composition comprises about 30% to about 35% by weight hop beta acids, about 10% to about 29% by weight solvent, about 30% to about 35% by weight emulsifier, about 1% to about 6% by weight fumed silica, and further comprising about 5% by weight ascorbic acid.

21. The composition of claim 18, wherein the composition is in the form of a liquid, a strip, or a paste.

22. The composition of claim 18, wherein the composition comprises about 1% to about 6% by weight fumed silica.

23. The composition of claim 18, wherein the fumed silica maintains composition availability for bee uptake or reduces beta acid degradation or reduces bee agitation by minimum dripping on bees in beehives.

24. The composition of claim 18, wherein the composition further comprises about 3% to about 5% by weight ascorbic acid.

25. The composition of claim 24, wherein the ascorbic acid reduces beta acid degradation.

26. The composition of claim 18, further comprising ascorbic acid.

27. The composition of claim 18, wherein the composition is active against parasitic mites.

Description

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS

(1) FIG. 1 is a picture showing the oxidative conversion of hop beta acid, lupulone, to degradation product hulupone in the presence of heat and oxygen.

(2) FIG. 2 provides pictures showing different formulations of HopGuard® III and various delivery methods.

(3) FIG. 3 is a graph showing hop beta acid degradation in HopGuard® II strips under simulated beehive conditions (35° C., 35% RH) over a period of 8 days.

(4) FIG. 4 and FIG. 5. are graphs showing hop beta acid degradation in HopGuard® II and HopGuard® II+5% Ascorbic acid (antioxidant strips) under simulated beehive conditions (35° C., 35% RH) and actual beehives, respectively. For simulated beehive conditions, the strips were collected on day 1, 3 and 7 and for actual beehive, the strips were collected on day 0 and 14.

(5) FIG. 6 is a graph showing weight of strip based products, HopGuard® II and HopGuard® III strips under simulated in-hive conditions (35° C., 35% RH) on day 0 and day 14.

(6) FIG. 7 is a graph showing hop beta acid oxidation of strip based products, HopGuard® II and HopGuard® III under simulated in-hive conditions (35° C., 35% RH) over a period of 30 days.

(7) FIG. 8 are pictures showing chewing bee behavior and strip condition for HopGuard® II and HopGuard® III strips on day 14 in beehives.

(8) FIG. 9 is a graph showing weights of different delivery products under simulated in-hive conditions (35° C., 35% RH) on day 0 and day 14.

(9) FIG. 10 is a graph showing hop beta acid degradation of different delivery products under simulated in-hive conditions (35° C., 35% RH) on day 0 and day 14.

EXAMPLES

Example 1

Development of HopGuard® Formulations and Testing Protocol

(10) Five Hop formulations and one control formulation with zero hop beta acid were developed at Haas Innovations Center, John I Haas, Inc., Yakima, Wash. TABLE. 1 is a table showing the composition of various HopGuard® formulations

(11) TABLE-US-00001 TABLE 1 Hop Beta Propylene Polysor- Fumed Ascorbic S. Acid Resins Glycol bate 60 Silica acid no. Product (%) (%) (%) (%) (%) 1 HopGuard 33 33 33 0 0 II 2 HopGuard 33 28 33 0 5 II + 5% Ascorbic acid 3 HopGuard 33 10 55.25 1.75 0 III/ HopGuard III Strip 4 HopGuard 33 10 52 5 0 III Paste 5 HopGuard 33 10 42 15 0 III Patty 6 Control 0 50 50 0 0

(12) In one embodiment, natural antioxidant ascorbic acid was used at the rate of 5% in the formulation while reducing the propylene glycol content to develop HopGuard® II+5% ascorbic acid strips. Other natural antioxidants such as, for example, vitamin A, tocopherols, carotenoids, lutein, lycopene, polyphenols like flavonoids or synthetic antioxidants such as propyl gallate, tertiary butylhydroquinone, butylated hydroxyanisole, and butylated hydroxytoluene could be used for the same purpose.

(13) Excipient fumed silica, a thixotropic material, was used to form gel, colloids and paste that convert into fluid when agitated due to bee interaction. The consistency of HopGuard® formulations was found to change with the level of excipient fumed silica added to the formulation, which allowed development of different delivery products such as liquid, paste, gel, patty or powder. Other materials with similar properties such as, for example, microparticles of alumina, aluminum nitride, carbon black, and nanocarbons, etc. could be used to achieve the desired outcome.

(14) Three formulations, HopGuard® II, HopGuard® II+5% Ascorbic acid and HopGuard® III were prepared by soaking corrugated cardboard strips, with mylar backing, 17.5 (L)×1.25 (B) folded in half, with liquid formulation added at the rate of 25 g per strip. Strips were placed in an aluminum foil bag (13×5 inches) and soaked in formulation for 24 hours. HopGuard® III paste formulation was added to a permeable bag at the rate of 25 g per bag (FIG. 2). HopGuard® III patty formulation was developed into a 25 g patty and placed on an impervious cardboard sheet.

(15) In a preferred embodiment, cardboard strip products are intended to be hung between the frames of beehives, whereas, paste and patty formula are intended to be placed on top of the frames or any other area where bees could interact with the product, for example the entrance.

(16) For testing of formulations in the lab, a Thermolyne (oven series 9000) hot air incubator was used to simulate beehive environment with a controlled temperature of 35° C. and relative humidity of 35%. The strips were hung in the incubator, whereas the paste and patty based formulas were placed on a wire rack. Samples were pulled out in triplicate on a given time point, weighed, placed in Ziploc bags, flushed with nitrogen gas, and stored under nitrogen flush at 5° C. until analysis for beta acid using HPLC as μg/inch.sup.2 for strips and μg/g for paste and patty products.

(17) For testing of formulations in the beehives, the products were tested in full strength bee colonies at Carl Hayden Bee Research Facility in Tucson, Ariz. For strip based formulas, two strips (25 g liquid formulation per strip) per hive were hung over the center brood frame near the middle of the frame with one half of the strip on each side of the frame. The paste and patty based products were placed on top of the center brood frames at the rate of two permeable bags (25 g each) or two patties (25 g each) per hive. Samples were pulled out in triplicate on a given time point, placed in Ziploc bags, flushed with nitrogen gas and stored under nitrogen flush at 5° C., until analysis for beta acid using HPLC.

Example 2

Inclusion of Antioxidant/s in the HopGuard® Formula Decreases Oxidative Degradation of Hop Beta Acid

(18) In a preliminary experiment, our currently available product, HopGuard® II, was found to have 30% beta acid degradation over a period of 8 days under simulated hive conditions as described above (FIG. 3). When tested in lab, using protocol described in example 1, the HopGuard® II+5% Ascorbic acid strips were found to have no beta acid degradation (p<0.05) over a period of 7 days, where 35% degradation of beta acid was observed in the HopGuard® II product (FIG. 4). When tested in beehives, the HopGuard® II+5% Ascorbic acid strips were found to have relatively lower beta acid degradation (44% degradation) when compared with HopGuard® II product (75% degradation) over a period of 14 days (FIG. 5).

Example 3

Addition of Excipient Fumed Silica Prolongs Availability of Product from HopGuard® Strips and Retards Oxidative Degradation of Beta Acids

(19) Two strip based products, HopGuard® II and HopGuard® III were tested for change in strip weight and beta acid degradation under simulated hive conditions over a period of 14 days using protocol described in Example 1. Strip weight and wetness were used as an indicator of the availability of product for bee uptake. Fumed silica in HopGuard® III strip prevented the reduction of strip weight via evaporative drying or drip loss, while keeping the strip wet on touch (observation), thereby enabling the strip to last longer (FIG. 6). On the other hand, HopGuard® II strips were completely dry (observation) and had a relatively greater change in weight over a period of 14 days. When tested for beta acid content, HopGuard® II strips had higher degradation of beta acid (41%) compared to HopGuard® III strips (30%) after 30 days (FIG. 7).

(20) The strips were tested in bee hives to observe bee response to HopGuard® II and HopGuard® III over a period of 14 days. HopGuard® III strips were found wet on touch (observation) and visibly intact on day 14 whereas HopGuard® II strips were completely dry and partially or completely chewed up by the bees with mylar film exposed (FIG. 8). HopGuard® III strips were found to cause no bee agitation (which is related to the product) in the beehives due to negligible product dripping unlike HopGuard® II strips which dripped on the bees and in and around the beehive causing bee agitation. There was no or negligible bee repellency to either of the products tested.

Example 4

Excipient Fumed Silica Based HopGuard® III Paste and Patty Delivery Prolongs Availability of Product and Prevents Oxidative Degradation of Beta Acids

(21) Two formulations, HopGuard® III paste and patty were tested for change in product weight and beta acid degradation under simulated hive conditions over a period of 14 days as described in Example 1. Strip weight and wetness were used an indicator of the availability of product for bee uptake. Both weight and beta acid content did not change (P<0.05) in the products, instead beta acid content of HopGuard® III paste was found to increase by 38% on day 14, probably due to the concentration of beta acid (FIG. 9, 10). Similar to HopGuard® III strips as discussed in example 3, HopGuard® III paste and patty product performed good and perhaps better in the bee hives.

(22) The various embodiments described above can be combined to provide further embodiments. These and other changes can be made to the embodiments in light of the above-detailed description. In general, in the following claims, the terms used should not be construed to limit the claims to the specific embodiments disclosed in the specification and the claims, but should be construed to include all possible embodiments along with the full scope of equivalents to which such claims are entitled. Accordingly, the claims are not limited by the disclosure.