CRYO-CARRIER

Abstract

There is disclosed a cryo-carrier comprising a carrier region having a plurality of grids, the carrier region having an embryo placement portion for supporting at least one embryo and a non-embryo placement portion surrounding the embryo placement portion. The non-embryo placement portion is filled with a support sheet so that it is stronger than the embryo placement portion, thereby supporting it.

Claims

1. A cryo-carrier for freezing at least one embryo, comprising: a carrier region having a plurality of grids, the carrier region having an embryo placement portion for supporting the at least one embryo and a non-embryo placement portion surrounding the embryo placement portion; the non-embryo placement portion being filled with a support sheet so that the non-embryo placement portion is stronger than the embryo placement portion, thereby supporting the embryo placement portion.

2. A cryo-carrier according to claim 1, wherein the thickness of the plurality of grids is less than one tenth of the diameter of the at least one embryo.

3. A cryo-carrier according to claim 1, wherein the carrier region is made of a nylon mesh.

4. A cryo-carrier according to claim 3, wherein the thickness of the nylon mesh in the embryo placement portion ranges from 1 to 40 μm.

5. A cryo-carrier according to claim 1, wherein wire inter-spacing of the plurality of grids is less than one third of the diameter of the at least one embryo.

6. A cryo-carrier according to claim 1, wherein inter-spacing of the plurality of grids in the embryo placement portion ranges from 36 to 50 μm.

7. A cryo-carrier according to claim 1, wherein the area of the embryo placement portion ranges from 300 to 1000 μm by 300 to 1000 μm.

8. A cryo-carrier according to claim 1, wherein the thickness of the support sheet is greater than or equal to the thickness of the plurality of grids.

9. A cryo-carrier according to claim 1, wherein the support sheet has a thickness that ranges from 10 to 100 μm.

10. A cryo-carrier according to claim 1, wherein the cryo-carrier further comprises a grip rod connected at an end of the carrier region.

Description

BRIEF DESCRIPTION OF THE FIGURES

[0020] Embodiments of the disclosure will be described by way of examples only with reference to the accompanying drawing, in which:

[0021] FIG. 1 is a schematic cross-sectional diagram of an embryo placed on a prior art plastic sheet;

[0022] FIG. 2 is a schematic cross-sectional diagram of an embryo placed on a prior art nylon mesh;

[0023] FIG. 3 is a schematic structural diagram of a cryo-carrier according to an illustrative embodiment of the present disclosure;

[0024] FIG. 4 is a schematic structural diagram of the carrier region of the cryo-carrier of FIG. 3; and

[0025] FIG. 5 is a schematic cross-sectional diagram of an embryo placed on the embryo placement portion of the carrier region of FIG. 4.

[0026] Similar references used in different Figures denote similar components.

DETAILED DESCRIPTION

[0027] Generally stated, the non-limitative illustrative embodiments of the present disclosure provide a cryo-carrier, which comprises a carrier region formed by a plurality of grids, wherein the carrier region comprises an embryo placing portion and a non-embryo placing portion, and the non-embryo placing portion surrounds the embryo placing portion. In the non-embryo placing portion, each grid is filled with a supporting sheet so that the hardness of the non-embryo placing portion is stronger than that of the embryo placing portion, thereby supporting the embryo placing portion. This structure ensures a uniform cooling rate.

[0028] Referring to FIGS. 3 and 4, there is shown the cryo-carrier according to an illustrative embodiment of the present disclosure, the cryo-carrier comprises a carrier region 3 consisting of a plurality of grids 30. For example, in the present illustrative embodiment, the carrier region 3 is formed by a nylon mesh. The nylon mesh refers to a net structure formed by a plurality of nylon wires in a staggered way.

[0029] The carrier region 3 includes an embryo placement portion A for placing one or more embryo and a non-embryo placement portion B, wherein the non-embryo placement portion B surrounds the embryo placement portion A in order to provide support. The embryo placement portion A is schematically encircled by a dashed box FIGS. 3 and 4, the number of grids in the embryo placement portion A not representing its actual number.

[0030] In the non-embryo placement portion B, each of the grids 30 is filled with a support sheet 31 so that the hardness of the non-embryo placement portion B is greater than that of the embryo placement portion A, thereby supporting the embryo placement portion A. In FIGS. 3 and 4, the support sheet 31 is schematically depicted by hatched lines. In particular, the support sheet 31 may be formed in the grid 30 of the non-embryo placement portion B using methods such as 3D printing or mold pouring, and the materials of the support sheet 31 include, but are not limited to, thermoplastic materials such as plastics, nylon, and the like. In the non-embryo placement portion B, due to the existence of the support sheet 31, a plurality of grids 30 are firmly connected with each other, which results in the hardness of the non-embryo placement portion B being higher than that of the embryo placement portion A.

[0031] In the illustrative embodiment the thickness of the support sheet 31 is greater than or equal to the thickness of the grid 30 to further increase the hardness of the non-embryo placement portion B. The thickness of the supporting sheet 31 in the illustrative embodiment may vary from 10 to 100 μm, including all values and sub ranges in between. However, in alternative embodiments the thickness of the supporting sheet 31 may vary in accordance with the thickness and/or wire inter-spacing of grids designed for specific applications (for example, for supporting various types of animal embryos, which differ in size compared to human embryos).

[0032] Referring to FIG. 5, there is shown a cross-sectional diagram of an embryo 10 placed on the embryo placement portion A. The cryo-carrier having a grid structure, the contact area of the embryo 10 and the grid 30 is small, the adhesion force is small, and holes are arranged below the embryo 10, so that the embryo 10 can be in contact with thawing solution, therefore, the embryo 10 is easy to float in the thawing solution, and the thawing solution can be moved to make the thawing solution in the hole below the embryo 10 flush the embryo 10, so that the embryo 10 can be easily separated from the cryo-carrier, and the thawing solution can be easily replaced and removed, so that the cryo-carrier of the can be very conveniently applied to the automatic technology of embryo freezing and thawing.

[0033] In the illustrative embodiment, the area of the embryo placement portion A is from 300 to 1000 μm by 300 to 1000 μm, including all values and sub ranges in between. This represents an advantage over prior art embryo carrier rods that have an embryo placement area of about 2000 μm by 5000 μm, which require decreasing the magnification of the microscope or moving the carrier rod in order to bring the embryos into the visual field of the microscope. The area of the embryo placement portion A of the cryo-carrier, and thus the visual field, are greatly reduced, so that the magnification of the microscope can be increased to clearly see the embryo, thereby realizing easy positioning.

[0034] In the illustrative embodiment, the wire inter-spacing of the grid 30 is in the range of 38 μm to 50 μm, including all values and sub ranges in between, and preferably less than one third of the diameter of the embryo to be placed on the embryo placement portion A. Furthermore, in the illustrative embodiment the thickness of the grid 30 is in the range of 1 μm to 40 μm, including all values and sub ranges in between, and preferably less than one tenth of the diameter of the embryo to be placed on the embryo placement portion A. It is to be understood that in alternative embodiments the wire inter-spacing and the thickness of the grid 30 may vary depending on the application.

[0035] Referring still to FIG. 5, since the thickness of the grid 30 is small and the wire inter-spacing of the grid 30 is less than the diameter of the embryo 10, the lower portion of the embryo 10 may be probed below the mesh after the embryo 10 is placed in the embryo placement portion A, no, bubbles are generated between the bottom of the embryo 10 and the grid 30, and the lower part of the embryo 10 is directly in contact with liquid nitrogen during the freezing operation, i.e. no insulating layer is formed, thereby increasing the cooling rate. In addition, since the embryo 10 is disposed on the grid 30, the grid 30 can transfer heat in four directions, further increasing the cooling rate.

[0036] Referring back to FIG. 3, in the illustrative embodiment the cryo-carrier further includes a grip rod 4 connected to the carrier region 3.

[0037] When the cryo-carrier is in use, such as when it is displaced, a user can hold the grip rod 4 to operate the cryo-carrier. The cryo-carrier also includes other structures conventionally used in the art, such as a sleeve for protecting the carrier region 3, and the like (not shown).

Sample Applications

[0038] Application 1: Manual artificial freezing and thawing of embryos. [0039] 1. Artificial freezing of embryos: The embryos are placed in the embryo placement area and excess cooling solution VS is aspirated from below with a tissue, the cryo-carrier is put into liquid nitrogen and cryopreserved. [0040] 2. Artificial thawing of embryos: The cryo-carrier is removed from liquid nitrogen and inserted into a thawing solution. The embryo is automatically detached from the cryo-carrier, or the thawing solution is reciprocally moved left and right to detach the embryo from the cryo-carrier.

[0041] Application 2: Freeze and haw tissue. [0042] 1. Freeze tissue: The tissue is placed in the embryo placement area and excess cooling solution VS is aspirated from below with a tissue, the cryo-carrier is put into liquid nitrogen and cryopreserved. [0043] 2. Thawing tissue: The cryo-carrier is removed from liquid nitrogen and thawed by insertion into a thawing solution.

[0044] Application 3: Embryo freezing automation. [0045] 1. The embryo is placed in the embryo placement portion A, the cryo-carrier is turned 180 degrees and cooling solution ES is added unto the embryo placement region A from above. [0046] 2. Wait for 5-15 minutes and add cooling solution VS unto the embryo placement region A from above. [0047] 3. Wait for about 30 seconds and remove the cooling solution from above with a tissue. [0048] 4. Add cooling solution VS from above. [0049] 5. The cryo-carrier is turned 180 degrees and adding cooling solution VS is added from above. [0050] 6. Wait for about 1 minute and the cooling solution is aspirated from below with a tissue. [0051] 7. The cryo-carrier is put into liquid nitrogen and cryopreserved.

[0052] It is to be understood that the cryo-carrier may also be used to freeze other biological matter, such as, for example, tissues.

[0053] Although the present disclosure has been described with a certain degree of particularity and by way of an illustrative embodiment and examples thereof, it is to be understood that the present disclosure is not limited to the features of the embodiments described and illustrated herein, but includes all variations and modifications within the scope and spirit of the disclosure as hereinafter claimed.