Silver ion bacteriostatic hand sanitizer and preparation method and application thereof

Abstract

The present disclosure relates to the technical field of sanitary cleaning products, and more specifically to a silver ion bacteriostatic hand sanitizer and preparation method and application thereof. The silver ion bacteriostatic hand sanitizer is provided, including: 0.01˜0.03 parts by weight of silver, 0.02˜0.08 parts by weight of silver sulfate, 1˜5 parts by weight of humectant, 10˜18 parts by weight of skin conditioning agent, 0.3˜0.8 parts by weight of preservative, 0.1˜0.5 parts by weight of sodium dodecyl benzene sulfonate, 0.8˜1.8 parts by weight of ethylhexylglycerol, 1˜5 parts by weight of propylene glycol, 3˜6 parts by weight of NONOXYNOL-14, 1˜6 parts by weight of PEG-50 castor oil, 0.01˜0.05 parts by weight of flavor and 80˜120 parts by weight of deionized water. The silver ion bacteriostatic hand sanitizer has the advantages of high-efficiency bacteriostasis, sterilization and itching relief, mild performance and no stimulation, and keeps the hands moisturized.

Claims

1. A silver ion bacteriostatic hand sanitizer, comprising: 0.02 parts by weight of silver, 0.05 parts by weight of silver sulfate, 3 parts by weight of methyl propanediol, 12 parts by weight of skin conditioning agent, 0.5 parts by weight of phenoxyethanol, 0.3 parts by weight of sodium dodecyl benzene sulfonate, 1.2 parts by weight of ethylhexylglycerol, 3 parts by weight of propylene glycol, 4 parts by weight of NONOXYNOL-14, 4 parts by weight of PEG-50 castor oil, 0.02 parts by weight of flavor and 100 parts by weight of deionized water; and wherein the skin conditioning agent comprises licorice extract, Artemisia annua extract and sodium citrate in a weight ratio of 6:13:2.

Description

DETAILED DESCRIPTION OF EMBODIMENTS

(1) Certain exemplary embodiments will now be described to provide an overall understanding of the principles of the structure, function, manufacture, and use of the instruments and methods disclosed herein. One or more examples of these embodiments are illustrated in the accompanying drawings. Those skilled in the art will understand that the instruments and methods specifically described herein and illustrated in the accompanying drawings are non-limiting exemplary embodiments and that the scope of the present disclosure is defined solely by the claims. The features illustrated or described in connection with one exemplary embodiment may be combined with the features of other embodiments. Such modifications and variations are intended to be included within the scope of the present disclosure.

(2) The liquorice extract was purchased from Nanjing Bangnuo biotech Co., Ltd. and the Artemisia annua extract was purchased from Shanghai Lianmai Bioengineering Co., Ltd.

(3) The remaining reagents used in the present disclosure are all common reagents and can be purchased from conventional reagent production and sales companies.

Embodiment 1 a Silver Ion Bacteriostatic Hand Sanitizer

(4) The silver ion bacteriostatic hand sanitizer is provided including: 0.01 parts by weight of silver, 0.02 parts by weight of silver sulfate, 1 parts by weight of methyl propanediol, 10 parts by weight of skin conditioning agent, 0.3 parts by weight of phenoxyethanol, 0.1 parts by weight of sodium dodecyl benzene sulfonate, 0.8 parts by weight of ethylhexylglycerol, 1 parts by weight of propylene glycol, 3 parts by weight of NONOXYNOL-14, 1 parts by weight of PEG-50 castor oil, 0.01 parts by weight of flavor and 80 parts by weight of deionized water.

(5) The skin conditioning agent includes licorice extract, Artemisia annua extract and sodium citrate in a weight ratio of 4:15:3.

(6) The preparation method for the silver ion bacteriostatic hand sanitizer is provided, including:

(7) S1: heating deionized water to 7011, stirring the heated deionized water and adding methyl propanediol, sodium dodecyl benzene sulfonate, ethylhexylglycerin, propylene glycol, NONOXYNOL-14 and PEG-50 castor oil into the heated deionized water at the same time to obtain a mixture, and stirring the mixture at a constant temperature for 20 min at a rotation speed of 800 r/min until added methyl propanediol, sodium dodecyl benzene sulfonate, ethylhexylglycerin, propylene glycol, NONOXYNOL-14 and PEG-50 castor oil completely dissolved to obtain mixed solution I;

(8) S2: cooling the mixed solution I obtained in step S1 to 20° C., adding silver, silver sulfate, skin conditioning agent, preservative and flavor into the mixed solution I, and homogenizing 20 min at a pressure of 8 MPa and a rotation speed of 2000 r/min to obtain the hand sanitizer.

Embodiment 2 a Silver Ion Bacteriostatic Hand Sanitizer

(9) The silver ion bacteriostatic hand sanitizer is provided including: 0.02 parts by weight of silver, 0.05 parts by weight of silver sulfate, 3 parts by weight of methyl propanediol, 12 parts by weight of skin conditioning agent, 0.5 parts by weight of phenoxyethanol, 0.3 parts by weight of sodium dodecyl benzene sulfonate, 1.2 parts by weight of ethylhexylglycerol, 3 parts by weight of propylene glycol, 4 parts by weight of NONOXYNOL-14, 4 parts by weight of PEG-50 castor oil, 0.02 parts by weight of flavor and 100 parts by weight of deionized water.

(10) The skin conditioning agent includes licorice extract, Artemisia annua extract and sodium citrate in a weight ratio of 6:13:2.

(11) The preparation method for the silver ion bacteriostatic hand sanitizer is provided, including:

(12) S1: heating deionized water to 751, stirring the heated deionized water and adding methyl propanediol, sodium dodecyl benzene sulfonate, ethylhexylglycerin, propylene glycol, NONOXYNOL-14 and PEG-50 castor oil into the heated deionized water at the same time to obtain a mixture, and stirring the mixture at a constant temperature for 25 min at a rotation speed of 900 r/min until added methyl propanediol, sodium dodecyl benzene sulfonate, ethylhexylglycerin, propylene glycol, NONOXYNOL-14 and PEG-50 castor oil completely dissolved to obtain mixed solution I;

(13) S2: cooling the mixed solution I obtained in step S1 to 25° C., adding silver, silver sulfate, skin conditioning agent, preservative and flavor into the mixed solution I, and homogenizing 30 min at a pressure of 12 MPa and a rotation speed of 2300 r/min to obtain the hand sanitizer.

Embodiment 3 a Silver Ion Bacteriostatic Hand Sanitizer

(14) The silver ion bacteriostatic hand sanitizer is provided including: 0.03 parts by weight of silver, 0.08 parts by weight of silver sulfate, 5 parts by weight of methyl propanediol, 18 parts by weight of skin conditioning agent, 0.8 parts by weight of phenoxyethanol, 0.5 parts by weight of sodium dodecyl benzene sulfonate, 1.8 parts by weight of ethylhexylglycerol, 5 parts by weight of propylene glycol, 6 parts by weight of NONOXYNOL-14, 6 parts by weight of PEG-50 castor oil, 0.05 parts by weight of flavor and 120 parts by weight of deionized water.

(15) The skin conditioning agent includes licorice extract, Artemisia annua extract and sodium citrate in a weight ratio of 9:8:1.

(16) The preparation method for the silver ion bacteriostatic hand sanitizer is provided, including:

(17) S1: heating deionized water to 80□, stirring the heated deionized water and adding methyl propanediol, sodium dodecyl benzene sulfonate, ethylhexylglycerin, propylene glycol, NONOXYNOL-14 and PEG-50 castor oil into the heated deionized water at the same time to obtain a mixture, and stirring the mixture at a constant temperature for 30 min at a rotation speed of 1000 r/min until added methyl propanediol, sodium dodecyl benzene sulfonate, ethylhexylglycerin, propylene glycol, NONOXYNOL-14 and PEG-50 castor oil completely dissolved to obtain mixed solution I;

(18) S2: cooling the mixed solution I obtained in step S1 to 30° C., adding silver, silver sulfate, skin conditioning agent, preservative and flavor into the mixed solution I, and homogenizing 60 min at a pressure of 15 MPa and a rotation speed of 2500 r/min to obtain the hand sanitizer.

(19) Control Group 1 a Silver Ion Bacteriostatic Hand Sanitizer

(20) The silver ion bacteriostatic hand sanitizer is provided including: 0.02 parts by weight of silver, 0.05 parts by weight of silver sulfate, 3 parts by weight of methyl propanediol, 12 parts by weight of skin conditioning agent, 0.5 parts by weight of phenoxyethanol, 0.3 parts by weight of sodium dodecyl benzene sulfonate, 1.2 parts by weight of ethylhexylglycerol, 3 parts by weight of propylene glycol, 4 parts by weight of NONOXYNOL-14, 4 parts by weight of PEG-50 castor oil, 0.02 parts by weight of flavor and 100 parts by weight of deionized water.

(21) The skin conditioning agent includes licorice extract and sodium citrate in a weight ratio of 6:2.

(22) The preparation method for the silver ion bacteriostatic hand sanitizer is similar to that of Embodiment 2.

(23) The difference from Embodiment 2 is that no Artemisia annua extract is added to the skin conditioning agent.

(24) Control Group 2 a Silver Ion Bacteriostatic Hand Sanitizer

(25) The silver ion bacteriostatic hand sanitizer is provided including: 0.02 parts by weight of silver, 0.05 parts by weight of silver sulfate, 3 parts by weight of methyl propanediol, 12 parts by weight of skin conditioning agent, 0.5 parts by weight of phenoxyethanol, 0.3 parts by weight of sodium dodecyl benzene sulfonate, 1.2 parts by weight of ethylhexylglycerol, 3 parts by weight of propylene glycol, 4 parts by weight of NONOXYNOL-14, 4 parts by weight of PEG-50 castor oil, 0.02 parts by weight of flavor and 100 parts by weight of deionized water.

(26) The skin conditioning agent includes licorice extract and Artemisia annua in a weight ratio of 6:13.

(27) The preparation method for the silver ion bacteriostatic hand sanitizer is similar to that of Embodiment 2.

(28) The difference from Embodiment 2 is that no sodium citrate is added to the skin conditioning agent.

(29) Control Group 3 a Silver Ion Bacteriostatic Hand Sanitizer

(30) The silver ion bacteriostatic hand sanitizer is provided including: 0.02 parts by weight of silver, 0.05 parts by weight of silver sulfate, 3 parts by weight of methyl propanediol, 12 parts by weight of skin conditioning agent, 0.5 parts by weight of phenoxyethanol, 0.3 parts by weight of sodium dodecyl benzene sulfonate, 1.2 parts by weight of ethylhexylglycerol, 3 parts by weight of propylene glycol, 4 parts by weight of NONOXYNOL-14, 4 parts by weight of PEG-50 castor oil, 0.02 parts by weight of flavor and 100 parts by weight of deionized water.

(31) The skin conditioning agent includes licorice extract, Artemisia annua extract and sodium citrate in a weight ratio of 1:1:1.

(32) The preparation method for the silver ion bacteriostatic hand sanitizer is similar to that of Embodiment 2.

(33) The difference from Embodiment 2 is that the skin conditioning agent includes licorice extract, Artemisia annua extract and sodium citrate in a weight ratio of 1:1:1.

Test Example 1 Bacteriostatic Test

(34) 1. Test material: the silver ion bacteriostatic hand sanitizer prepared in Embodiment 1˜3, Control group 1˜3.

(35) 2. Test subjects: Escherichia coli, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa.

(36) 3. Test methods: bacteriostatic experiment: 3 ml of the silver ion bacteriostatic hand sanitizer prepared for Embodiment 1˜3 and Control group 1˜3 was placed inside conical flasks respectively, and then 70 ml phosphate buffer (0.03 mol.Math.L-1) and 5 ml bacteria solution were added, respectively, after the liquid was infiltrated into the medium, and after incubation at 37° C. for 2 min, colony counting was performed.

(37) The bacteriostatic rate was calculated by the formula: X=(A−B)/A×100%, wherein X was the bacteriostatic rate, A was the average number of colonies before the test specimen was shaken, and B was the average number of colonies after the test specimen was shaken.

(38) 4. Test result

(39) The test results are shown in Table 1.

(40) TABLE-US-00001 TABLE 1 Results of the silver ion bacteriostatic hand sanitizer bacteriostatic test Bacteriostatic rate (%) Escherichia Candida Staphylococcus Pseudomonas Group coli albicans aureus aeruginosa Embodiment 1 >99.9 >99.9 >99.9 >99.9 Embodiment 2 >99.9 >99.9 >99.9 >99.9 Embodiment 3 >99.9 >99.9 >99.9 >99.9 Control group 1 64.31 65.17 66.17 52.19 Control group 2 80.33 79.36 77.25 70.21 Control group 3 82.66 83.57 83.74 77.32

(41) It is known from Table 1 that the silver ion bacteriostatic hand sanitizer prepared in Embodiment 1˜3 had more than 99.9% bacteriostatic rate against Escherichia coli, Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa within 2 min, indicating that the silver ion bacteriostatic hand sanitizer prepared in this work had good bacteriostatic effect. While the test results on the Control group 1˜3 with the Embodiments showed that when the components of the skin conditioning agent provided by the present disclosure were changed, the bacteriostatic effect of the prepared silver ion bacteriostatic hand sanitizer within 2 min decreased obviously, indicating that the components of the skin conditioning agent provided by the present disclosure were indispensable and the synergy was obvious.

Test Example 2 Determination of Stability

(42) 1. Test material: the silver ion bacteriostatic hand sanitizer prepared in Embodiment 2, Control group 1˜3.

(43) 2. Test contents: 5 g of the silver ion bacteriostatic hand sanitizer prepared in Embodiment 2, Control group 1˜3, were placed into an incubator, all treated for 24 h and removed. When the temperature returned to room temperature, the results were observed.

(44) 3. Test result

(45) The test results are shown in Table 2.

(46) TABLE-US-00002 TABLE 2 Stability test results Testing condition Group Phenomenon Heat resistance Embodiment No turbidity, no floccule, uniform ( 40 ± 1□) 2 non-layered, no peculiar smell Control Slight turbidity, no floccule, uniform group 1 non-layered, no peculiar smell Control Serious turbidity, floccule, solution group 2 layered, no peculiar smell Control Slight cloudiness, no floccule, uniform group 3 non-layered, no peculiar smell Cold resistance Embodiment No turbidity, no floccule, uniform ( −5~0□ ) 2 non-layered, no peculiar smell Control Slight turbidity, no floccule, solution group 1 layered, no peculiar smell Control Slight turbidity, floccule, solution group 2 layered, no peculiar smell Control Slight turbidity, floccule, solution group 3 layered, no peculiar smell

(47) It can be known from Table 2 that under the detection conditions of heat resistance (40±1□) and cold resistance (−5˜0□), the silver ion bacteriostatic hand sanitizer prepared in Embodiment 2 has no change in smell and color, no precipitation and suspended solids, and the texture is uniform, indicating that the hand sanitizer of the disclosure is in stable state. In the Control group 1˜3, when the composition of skin conditioning agent was changed, the silver ion bacteriostatic hand sanitizer appeared turbid and flocculent, and the solution was layered under the same condition.

(48) Described above are merely illustrative of the disclosure to enable those skilled in the art to implement or use the disclosure, and are not intended to limit the invention. It should be understood that any modifications, replacements or changes made by those skilled in the art without departing from the spirit of the disclosure should fall within the scope of the disclosure.