Fungicidal composition

Abstract

A fungicidal composition comprising active components A and B, wherein the active component A is benzisothiazolinone, and the active component B is flutriafol; and the weight ratio of the two components is 1:30-30:1. Also provided are a preparation method and use of the composition. Test results show that the fungicidal composition provided in the present invention has an obvious synergistic effect, and more importantly, the application rate is reduced and the cost is reduced. The fungicidal composition is effective in controlling certain specific fungal diseases of the crops. Through the combination of different fungicides with different mechanisms and modes of action, the application rate of each single agent is effectively reduced, and a good effect on broadening the fungicidal spectrum, retarding the fungal resistance and improving the control effect is exhibited.

Claims

1. A pesticidally acceptable formulation prepared with fungicidal active components and optionally a pesticide adjuvant or aid, wherein the fungicidal active components consist of active components A and B as the only active components, wherein the active component A is benzisothiazolinone, the active component B is flutriafol, the weight ratio between the two components is 1:30-30:1.

2. The pesticidally acceptable formulation according to claim 1, wherein the weight ratio of the active component A to the active component B is 1:20-20:1.

3. The pesticidally acceptable formulation according to claim 2, wherein the weight ratio of the active component A to the active component B is 1:10-10:1.

4. The pesticidally acceptable formulation according to claim 1, wherein the pesticide adjuvant or aid is present.

5. The pesticidally acceptable formulation according to claim 4, comprising 4-80 wt % of active components and 96-20 wt % of a pesticide adjuvant.

6. The pesticidally acceptable formulation according to claim 4, wherein the formulation is a wettable powder, a suspension, or water dispersible granules.

7. The pesticidally acceptable formulation according to claim 4, wherein the pesticide adjuvant or aid is one or more selected from a carrier, a solvent, a dispersant, a wetting agent, a binder, a thickener, an adhesive, a surfactant, and a fertilizer.

8. A method of controlling crop diseases in an agricultural area, comprising applying the pesticidally acceptable formulation according to claim 1 to the agricultural area.

9. The method according to claim 8, wherein the applying comprises controlling wheat leaf rust or strawberry powdery mildew in the agricultural area.

10. A pesticidally acceptable formulation prepared with fungicidal active components and optionally a pesticide adjuvant or aid, wherein the fungicidal active components consist of active component A and active component B wherein the active component A is benzisothiazolinone, the active component B is flutriafol, the weight ratio between the two components is 1:30-30:1 with the exclusion of 1:25.

Description

DETAILED DESCRIPTION

(1) To make the objects, the technical solution, and advantages of the present invention clearer, the present invention is described in further detail with reference to examples. It should be understood that the specific examples described herein are merely provided for illustrating, instead of limiting the present invention. Any modifications and equivalent improvements and substitutions can be made thereto without departing from the spirit and principle of the present invention, which are all fall within the protection scope of the present invention.

(2) The percentages given in all the formulations in the examples below are all weight percentages. The various formulations are processed from the composition of the present invention by a process known in the prior art which may be varied as desired.

I. Preparation Examples of Formulations

(I) Processing and Examples of Wettable Powder

(3) The active ingredients A and B and various additives and fillers were fully mixed in proportion, and ground by an ultrafine grinder to prepare a wettable powder.

Example 1: 52% Benzisothiazolinone.Math.Flutriafol Wettable Powder

(4) benzisothiazolinone 2%, flutriafol 50%, Nekal 5%, bentonite 3%, alkyl polyoxyethylene ether sulfonate 4%, white carbon black 5%, and diatomite q.s. to 100%.

Example 2: 44% Benzisothiazolinone.Math.Flutriafol Wettable Powder

(5) benzisothiazolinone 4%, flutriafol 40%, sodium ligninsulfonate 5%, alkyl polyoxyethylene ether sulfonate 6%, white carbon black 10%, and diatomite q.s. to 100%.

Example 3: 12% Benzisothiazolinone.Math.Flutriafol Wettable Powder

(6) benzisothiazolinone 10%, flutriafol 2%, sodium ligninsulfonate 5%, alkyl sulfonate 5%, white carbon black 10%, and Kaolin q.s. to 100%.

Example 4: 64% Benzisothiazolinone.Math.Flutriafol Wettable Powder

(7) benzisothiazolinone 60%, flutriafol 4%, soapy clay 3%, alkyl sulfonate 5%, white carbon black 5%, and diatomite q.s. to 100%.

Example 5: 31% Benzisothiazolinone.Math.Flutriafol Wettable Powder

(8) benzisothiazolinone 30%, flutriafol 1%, sodium benzoate 5%, alkyl polyoxyethylene ether sulfonate 6%, white carbon black 10%, and Kaolin q.s. to 100%.

(II) Processing and Examples of Suspensions

(9) The active ingredients A and B were uniformly mixed with a dispersing agent, a wetting agent, a thickener, water and other components in proportion, and ground and/or high-speed sheared to give a semi-finished product, which was analyzed, supplemented with water, mixed uniformly, and filtered, to obtain a finished product.

Example 6: 15.5% Benzisothiazolinone.Math.Flutriafol Suspension

(10) benzisothiazolinone 0.5%, flutriafol 15%, white carbon black 5%, ethylene glycol 5%, fatty alcohol polyoxyethylene ether phosphate 6%, and water q.s. to 100%.

Example 7: 32% Benzisothiazolinone.Math.Flutriafol Suspension

(11) benzisothiazolinone 2%, flutriafol 30%, white carbon black 3%, glycerol 4%, sodium benzoate 2%, sodium methyl naphthalene sulfonate-formaldehyde condensate 5%, and water q.s. to 100%.

Example 8: 10% Benzisothiazolinone.Math.Flutriafol Suspension

(12) benzisothiazolinone 5%, flutriafol 5%, bentonite 4%, ethylene glycol 5%, sodium benzoate 7%, Xantham gum 2%, and water q.s. to 100%.

Example 9: 21% Benzisothiazolinone.Math.Flutriafol Suspension

(13) benzisothiazolinone 20%, flutriafol 1%, bentonite 3%, glycerol 5%, sodium ligninsulfonate 4%, sodium methyl naphthalene sulfonate-formaldehyde condensate 5%, and water q.s. to 100%.

(III) Processing and Examples of Water Dispersible Granules

(14) The active components A and B, an adjuvant and a filler were mixed evenly according to the ratio of the formulation, comminuted by gas stream into a wettable powder, then added with an amount of water, mixed, extruded, granulated, dried, and sieved, to obtain a water dispersible granule product.

Example 10: 10.5% Benzisothiazolinone.Math.Flutriafol Water Dispersible Granules

(15) benzisothiazolinone 0.5%, flutriafol 10%, potassium dodecyl sulfonate 5%, ammonium sulfate 5%, potassium alkyl naphthalene sulfonate 4%, and light calcium carbonate q.s. to 100%.

Example 11: 30% Benzisothiazolinone.Math.Flutriafol Water Dispersible Granules

(16) benzisothiazolinone 5%, flutriafol 25%, sodium methyl naphthalene sulfonate-formaldehyde condensate 5%, sodium dodecyl sulfate 3%, sodium ligninsulfonate 6%, and diatomite q.s. to 100%.

Example 12: 22% Benzisothiazolinone.Math.Flutriafol Water Dispersible Granules

(17) benzisothiazolinone 20%, flutriafol 2%, potassium dodecyl sulfonate 3%, ammonium sulfate 5%, sodium methyl naphthalene sulfonate-formaldehyde condensate 5%, and light calcium carbonate q.s. to 100%.

Example 13: 52% Benzisothiazolinone.Math.Flutriafol Water Dispersible Granules

(18) benzisothiazolinone 50%, flutriafol 2%, sodium methyl naphthalene sulfonate-formaldehyde condensate 5%, sodium dodecyl sulfate 5%, ammonium sulfate 5%, and diatomite q.s. to 100%.

II. Efficiency Verification Test

(I) Bioassay Examples

(19) Based on the test grade scale, the disease development on the leaves of the whole cucumber plant was investigated, and the disease index and control effect were calculated.

(20) The control effect was converted into probability (y), the concentration of the agents (μg/ml) in solution was converted into a logarithmic value (x), the toxic regression equation and the median inhibition concentration EC50 were calculated by least square method, and the toxicity index and the co-toxicity coefficient (CTC) of the agents were calculated by SUN Peiyun method.
Actual toxicity index (ATI)=(EC50 of standard/EC50 of test agent)*100
Theoretical toxicity index (TTI)=toxicity index of agent A*percentage content of A in the mixture+toxicity index of agent B*percentage content of B in the mixture
Co-toxicity coefficient (CTC)=[actual toxicity index (ATI) of the mixture/theoretical toxicity index (TTI) of the mixture]*100

(21) ≥Where CTC≤80, the composition exhibits an antagonistic effect; where 80<CTC<120, the composition exhibits an additive effect, and where CTC≥120, the composition exhibits a synergistic effect.

(22) 1. Toxicity Test of Benzisothiazolinone Combined with Flutriafol

(23) TABLE-US-00001 TABLE 1 Toxicity test result analysis of benzisothiazolinone combined with flutriafol on wheat leaf rust Co-toxicity coefficient Name of agent EC.sub.50 (μg/ml) ATI TTI (CTC) Flutriafol 67.34 100.00 \ \ Benzisothiazolinone 40.23 167.39 \ \ Benzisothiazolinone:flutriafol = 1:30 53.25 126.45 102.17 123.76 Benzisothiazolinone:flutriafol = 1:25 AM 52.15 129.13 102.59 125.87 Benzisothiazolinone:flutriafol = 1:20 AM 48.61 138.54 103.21 134.23 Benzisothiazolinone:flutriafol = 1:15 AM 46.94 143.47 104.21 137.67 Benzisothiazolinone:flutriafol = 1:10 AM 42.85 157.16 106.13 148.09 Benzisothiazolinone:flutriafol = 1:5 40.30 167.10 111.23 150.23 Benzisothiazolinone:flutriafol = 1:1 33.02 203.94 133.69 152.54 Benzisothiazolinone:flutriafol = 5:1 28.56 235.76 156.16 150.98 Benzisothiazolinone:flutriafol = 10:1 28.11 239.57 161.26 148.56 Benzisothiazolinone:flutriafol = 15:1 29.91 225.15 163.18 137.98 Benzisothiazolinone:flutriafol = 20:1 30.33 222.02 164.18 135.23 Benzisothiazolinone:flutriafol = 25:1 32.19 209.21 164.80 126.95 Benzisothiazolinone:flutriafol = 30:1 32.85 204.98 165.21 124.07

(24) The results (in Table 1) show that the control effect of the combination of benzisothiazolinone and flutriafol on wheat leaf rust is significantly improved, suggesting that the combination of the two components has an obvious synergistic effect on wheat leaf rust. Experiments show that when the ratio of benzisothiazolinone to flutriafol is beyond the range defined in the present invention, the co-toxicity coefficient is below 120.

(25) II. Field Efficiency Verification Test

(26) Test method: in early stage of disease development, the first spray was given immediately, and then the second application was given after 7 days. Each treatment included 4 plots of 20 square meters each. The disease development before application and 11 days after the second application was statistically investigated. Samples were collected from 5 locations in each plot at random, and 5 plants were investigated at each location by investigating the percentages of the disease spot area on the leaves relative to the leaf area of the whole plant and grading. The disease index and the control effect were calculated.

(27) $\mathrm{Disease}\mathrm{index}=\frac{\Sigma \left(\begin{array}{c}\mathrm{Number}\mathrm{of}\mathrm{leaves}\mathrm{at}\mathrm{each}\\ \mathrm{grade}\mathrm{of}\mathrm{disease}\mathrm{development}\end{array}\times \begin{array}{c}\mathrm{Representative}\mathrm{value}\\ \mathrm{of}\mathrm{corresponding}\mathrm{grade}\end{array}\right)}{\begin{array}{c}\mathrm{Total}\mathrm{number}\mathrm{of}\\ \mathrm{leaves}\mathrm{investigated}\end{array}\times \begin{array}{c}\mathrm{Representative}\mathrm{value}\\ \mathrm{of}\mathrm{highest}\mathrm{level}\end{array}}\times 100$$\mathrm{Control}\mathrm{effect}\left(\%\right)=\left(1-\frac{\begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{control}\\ \mathrm{group}\mathrm{before}\mathrm{application}\end{array}\times \begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{treatment}\\ \mathrm{group}\mathrm{after}\mathrm{application}\end{array}}{\begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{control}\\ \mathrm{group}\mathrm{after}\mathrm{application}\end{array}\times \begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{treatment}\\ \mathrm{group}\mathrm{before}\mathrm{application}\end{array}}\right)\times 100$

(28) Anticipated control effect (%)=X+Y−XY/100 (where X and Y are the control effect of a sigle agent)

(29) Grade Scale:

(30) Grade 0: no disease spot;

(31) Grade 1: number of disease spots on the leaf<5, and length<1 cm;

(32) Grade 3: 6≤number of disease spots on the leaf≤10, and length of some disease spots>1 cm;

(33) Grade 5: 11≤number of disease spots on the leaf≤25, some disease spots are contiguous, and the disease spot area is 10-25% of the leaf area;

(34) Grade 7: number of disease spots on the leaf≥26, the disease spots are contiguous, and the disease spot area is 26-50% of the leaf area;

(35) Grade 9: the disease spots are contiguous, and the disease spot area is above 50% of the leaf area, or all the leaves all wilted.

(36) 1. Field Efficacy Test of Benzisothiazolinone Combined with Flutriafol

(37) TABLE-US-00002 TABLE 2 Control effect of benzisothiazolinone combined with flutriafol on strawberry powdery mildew Disease Day 11 after the index second application Amount before Disease Control No. Agent (mg/kg) application index effect (%) Example 1 3% benzisothiazolinone wettable 4.6 15.36 30.55 6.07 powder 50% flutriafol wettable powder 115.4 14.98 11.23 64.58 Anticipated control efficacy after — — — 66.73 mixing them 52% benzisothiazolinone•flutriafol 120 16.02 9.82 71.05 wettable powder (benzisothiazolinone:flutriafol = 1:25) Example 2 3% benzisothiazolinone wettable 10.9 14.78 28.26 9.69 powder 80% flutriafol wettable powder 109.1 14.56 11.99 61.11 Anticipated control efficacy after — — — 64.88 mixing them 44% benzisothiazolinone•flutriafol 120 16.21 7.51 78.11 wettable powder (benzisothiazolinone:flutriafol = 1:10 AM) Example 3 3% benzisothiazolinone wettable 100.0 13.98 11.96 59.61 powder 50% flutriafol wettable powder 20.0 14.03 26.10 12.13 Anticipated control efficacy after — — — 64.51 mixing them 12% benzisothiazolinone•flutriafol 120 17.32 7.97 78.28 wettable powder (benzisothiazolinone:flutriafol = 5:1) Example 4 3% benzisothiazolinone wettable 112.5 10.78 7.58 66.81 powder 50% flutriafol wettable powder 7.5 16.43 32.97 5.23 Anticipated control efficacy after — — — 68.55 mixing them 64% benzisothiazolinone•flutriafol 120 11.37 5.40 77.57 wettable powder (benzisothiazolinone:flutriafol = 15:1) Example 5 3% benzisothiazolinone wettable 116.1 10.77 7.17 68.56 powder 80% flutriafol wettable powder 3.9 12.34 25.28 3.25 Anticipated control efficacy after — — — 69.58 mixing them 31% benzisothiazolinone•flutriafol 120 15.04 8.58 73.07 wettable powder (benzisothiazolinone:flutriafol = 30:1) Example 6 3% benzisothiazolinone suspension 3.9 12.22 24.38 5.78 25% flutriafol suspension 116.1 15.34 11.29 65.24 Anticipated control efficacy after — — — 67.25 mixing them 15.5% benzisothiazolinone•flutriafol 120 16.03 9.90 70.82 suspension (benzisothiazolinone:flutriafol = 1:30) Example 7 3% benzisothiazolinone suspension 7.5 13.98 27.29 7.81 40% flutriafol suspension 112.5 14.34 11.24 62.99 Anticipated control efficacy after — — — 65.88 mixing them 32% benzisothiazolinone•flutriafol 120 15.67 8.34 74.85 suspension (benzisothiazolinone:flutriafol = 1:15 AM) Example 8 3% benzisothiazolinone suspension 60.0 15.78 20.98 37.22 40% flutriafol suspension 60.0 15.33 21.37 34.15 Anticipated control efficacy after — — — 58.66 mixing them 10% benzisothiazolinone•flutriafol 120 10.89 6.12 73.44 suspension (benzisothiazolinone:flutriafol = 1:1) Example 9 3% benzisothiazolinone suspension 114.3 10.34 7.10 67.59 40% flutriafol suspension 5.7 14.98 30.37 4.25 Anticipated control efficacy after — — — 68.97 mixing them 21% benzisothiazolinone•flutriafol 120 14.44 6.88 77.50 suspension (benzisothiazolinone:flutriafol = 20:1) Example 10 3% benzisothiazolinone water 5.7 15.87 31.28 6.92 dispersible granules 35% flutriafol water dispersible 114.3 16.39 12.51 63.95 granules Anticipated control efficacy after — — — 66.44 mixing them 10.5% benzisothiazolinone•flutriafol 120 10.74 5.74 74.76 water dispersible granules (benzisothiazolinone:flutriafol = 1:20) Example 11 3% benzisothiazolinone water 20.0 13.77 24.84 14.79 dispersible granules 20% flutriafol water dispersible 100.0 15.98 14.85 56.12 granules Anticipated control efficacy after — — — 62.61 mixing them 30% benzisothiazolinone•flutriafol 120 16.22 8.04 76.59 water dispersible granules (benzisothiazolinone:flutriafol = 1:5) Example 12 3% benzisothiazolinone water 109.1 15.09 11.27 64.72 dispersible granules 20% flutriafol water dispersible 10.9 10.78 21.20 7.14 granules Anticipated control efficacy after — — — 67.24 mixing them 22% benzisothiazolinone•flutriafol 120 15.23 6.33 80.36 water dispersible granules (benzisothiazolinone:flutriafol = 10:1) Example 13 3% benzisothiazolinone water 115.4 16.89 11.35 68.27 dispersible granules 35% flutriafol water dispersible 4.6 15.34 31.30 3.64 granules Anticipated control efficacy after — — — 69.42 mixing them 52% benzisothiazolinone•flutriafol 120 16.67 9.28 73.69 water dispersible granules (benzisothiazolinone:flutriafol = 25:1) CK Water control — 15.42 32.65 —

(38) The test results (in Table 2) show that the control effect of the combination of benzisothiazolinone and flutriafol on strawberry powdery mildew is significantly improved, suggesting that the combination of the two components has an obvious synergistic effect on strawberry powdery mildew.