OIL-AND-FAT COMPOSITION

Abstract

The present invention addresses the problem of providing an ascorbic acid-containing oil and fat that allows improvement of ascorbic acid content in the ascorbic acid-containing oil and fat and that can be used for a variety of food items. In this oil-and-fat composition, an appropriate amount of ascorbic acid is contained, and the ascorbic acid is contained as an aqueous solution having a pH of 2.5-5.3.

Claims

1. A fat composition comprising an ascorbic acid at an amount of 50 to 50,000 mass ppm as ascorbic acid equivalent as an aqueous solution having a pH of 2.5 to 5.3.

2. The fat composition according to claim 1, comprising an oil-soluble emulsifier.

3. The fat composition according to claim 2, comprising a glycerin.

4. The fat composition according to claim 1, wherein an average particle size of an aqueous phase is 1,000 nm or less.

5. A food comprising the fat composition according to claim 1 at an amount of 100 to 5,000 mass ppm.

6. A method for producing a fat composition, the method comprising mixing an oil phase and an aqueous phase, wherein a pH of the aqueous phase is adjusted to 2.5 to 5.3, and wherein the aqueous phase is an aqueous solution containing an ascorbic acid at an amount of 50 to 50,000 mass ppm as ascorbic acid equivalent relative to the oil phase.

7. The method according to claim 6, wherein a water content is adjusted to 0.5 to 2.0 times a content of the ascorbic acid.

8. The method according to claim 6, wherein the oil phase contains an oil-soluble emulsifier.

9. The method according to claim 8, wherein the oil phase contains a glycerin.

10. A method for stably dispersing an aqueous ascorbic acid solution in a fat composition, comprising containing an ascorbic acid as an aqueous solution having a pH of 2.5 to 5.3 in the fat composition, wherein the fat composition contains an ascorbic acid at an amount of 50 to 50,000 mass ppm as ascorbic acid equivalent.

11. The fat composition according to claim 1, wherein a melting point of an oil phase is 20° C. or more.

12. The fat composition according to claim 2, wherein an average particle size of an aqueous phase is 1,000 nm or less.

13. The fat composition according to claim 3, wherein an average particle size of an aqueous phase is 1,000 nm or less.

14. A food comprising the fat composition according to claim 2 at an amount of 100 to 5,000 mass ppm.

15. A food comprising the fat composition according to claim 3 at an amount of 100 to 5,000 mass ppm.

16. A food comprising the fat composition according to claim 4 at an amount of 100 to 5,000 mass ppm.

17. A food comprising the fat composition according to claim 12 at an amount of 100 to 5,000 mass ppm.

18. A food comprising the fat composition according to claim 13 at an amount of 100 to 5,000 mass ppm.

19. The method according to claim 7, wherein the oil phase contains an oil-soluble emulsifier.

20. The method according to claim 19, wherein the oil phase contains a glycerin.

Description

MODE FOR CARRYING OUT INVENTION

[0043] Hereinafter, the present invention will be described in more detail.

[0044] A fat composition of the present invention contains an ascorbic acid at an amount of 50 to 50,000 mass ppm as ascorbic acid equivalent.

[0045] The fat composition of the present invention is adapted for a wide range of concentration from low concentration to high concentration. The content of ascorbic acid is preferably 50 to 40,000 mass ppm, more preferably, 50 to 35,000 mass ppm, and further preferably 50 to 30,000 mass ppm, as ascorbic acid equivalent.

[0046] The content of ascorbic acid is preferably 100 to 40,000 mass ppm, more preferably 500 to 35,000 mass ppm, and further preferably 1,000 to 30,000 mass ppm, as ascorbic acid equivalent, from the viewpoint of being capable of being dispersed at a high concentration.

[0047] As used herein, the ascorbic acid equivalent is calculated by converting it into a value as ascorbic acid contained therein, for example, when ascorbate is used. Specifically, when sodium ascorbate is used, with respect to this molecular weight of 198.11 g, the molecular weight of ascorbic acid is 176.13 g. Therefore, if sodium ascorbate is used at an amount of A g, it is converted to A×(176.13/198.11) as ascorbic acid. The calculated amount is referred to herein as ascorbic acid equivalent.

[0048] The fat composition of the present invention contains the ascorbic acid as an aqueous solution having a pH of 2.5 to 5.3. Preferably, the fat composition contains the ascorbic acid as an aqueous solution having a pH of 3.0 to 5.3. More preferably, it is 3.3 to 5.3. A dispersiblity of ascorbic acid in the fat is improved by adjusting within such a range. When the pH is less than 2.5, ascorbic acid may crystallize and precipitate under conditions of heating and stirring. Further, when the pH exceeds 5.3, a taste enhancing effect may be impaired.

[0049] The pH of the ascorbic acid aqueous solution may be adjusted by using various alkalis and weak acid salts or by combining ascorbic acid and ascorbate in a preparation of the aqueous phase. In addition, the pH of the aqueous solution of ascorbic acid may be adjusted by, dispersing an aqueous solution of ascorbic acid without adjusting pH into fat, and then dispersing various alkalis and weak acid salts or blending an oil-soluble basic substance such as fatty acid salt and sodium stearoyl lactate. Also in these cases, it can be considered that the ascorbic acid is contained as an aqueous solution within the adjusted pH range.

[0050] In a preferred aspect, the aqueous phase may be prepared quickly by using ascorbic acid and ascorbate in combination because pH adjustment becomes easy and the melting rate is fast.

[0051] At the time of preparing the aqueous phase, the ascorbic acid and ascorbate to be used must be dissolved. Whether or not it is dissolved is determined by, for example, confirming that precipitation does not occur when the solution is subjected to centrifugation. More specifically, when a precipitate is not visually confirmed after subjecting 5 ml aqueous phase placed in a 20 ml centrifuge tube to centrifugation at 3,000 G for 1 minute at room temperature, 25° C., it is assumed as substantially dissolved.

[0052] Examples of fats that may be used for the fat composition of the present invention include vegetable fats such as rapeseed oil, soybean oil, sunflower seed oil, cotton seed oil, groundnut oil, rice bran oil, corn oil, safflower oil, olive oil, kapok oil, sesame oil, evening primrose oil, palm oil, palm kernel oil, coconut oil, medium-chain fatty acid-containing fat (MCT), shea butter, and sal fat; animal fats such as milk fat, beef tallow, lard, fish oil, and whale oil; algae oil; and processed fat thereof such as hydrogenated oil, fractionated oil, hydrogenated fractionated oil, fractionated hydrogenated oil, and interesterified oil; and mixed fat thereof. The ascorbic acid is stably dispersed in the fat composition of the present invention even under conditions such as being held at a high temperature or being stirred. Thus, the composition may be used to fat without limitation also in a fat which has 20° C. or higher of melting point and needs to be stored at a high temperature before use.

[0053] In the fat composition of the present invention, a fat-soluble emulsifier is desirably dissolved in the oil phase. The fat-soluble emulsifier is an emulsifier that may be dissolved in fat, and refers to an emulsifier having an HLB of 7 or less in the present invention. As the fat-soluble emulsifier, one or more selected from polyglycerol 1.0 esters, sugar esters, sorbitan esters, and monoglycerin fatty acid esters are desirable, more desirably, polyglycerol esters, sugar esters, and distilled monoglycerides are preferred, particularly, polyglycerol esters are preferred, and polyglycerol condensed ricinoleic acid ester is most preferable among polyglycerol esters. Polyglycerol condensed ricinoleic acid ester may be abbreviated as PGPR. The amount of the fat-soluble emulsifier in the oil phase is preferably 0.1 to 10 times the amount of ascorbic acid, more preferably 0.3 to 7 times the amount of ascorbic acid, and further preferably 0.5 to times the amount of ascorbic acid. By using an appropriate amount of an appropriate emulsifier, the dispersion stability of ascorbic acid may be improved, and thereby the function of ascorbic acid-containing fat composition may be exerted. In case that the amount of the emulsifier is excessive, when the fat composition of the present invention is used for foods, decrease in eating quality due to flavor derived from the emulsifier and unintended inhibition of emulsification occur, which impairs the quality of foods.

[0054] The fat composition of the present invention preferably contains glycerin. When the composition contains glycerin, the ascorbic acid may be stably dispersed at a high concentration, which is preferable. In particular, when ascorbic acid is contained in fat at an amount of 1,000 mass ppm or more as ascorbic acid equivalent, glycerin is desirably contained. Even when the content of ascorbic acid is less than 1,000 mass ppm, the glycerin contributes to stable dispersion.

[0055] The content of the glycerin is preferably 0.01 to 10 times the amount of ascorbic acid, more preferably 0.1 to 7 times the amount of ascorbic acid, and further preferably 0.3 to 5 times the amount of ascorbic acid. A larger blended amount results in improved dispersion stability of ascorbic acid. However, excessive amount inhibits dispersion stability and causes precipitation of ascorbic acid.

[0056] An apparatus, a mixer or a stirrer, may be used for mixing an oil phase and an aqueous phase. The apparatus is not particularly limited, and an apparatus which may prevent contaminating air during mixing and stirring is preferable. A mixing and emulsifying apparatus such as a high pressure homogenizer may be exemplified.

[0057] The fat composition of the present invention is preferably adjusted such that the emulsified particle size is 1,000 nm or less. The emulsified particle size is more preferably 500 nm or less, and further preferably 300 nm or less. By adjusting the average emulsified particle size to 1,000 nm or less, ascorbic acid may be stably dispersed at a high concentration, which is preferable. When the average emulsified particle size exceeds 1,000 nm, the dispersion stability deteriorates, which is not preferable. In the present invention, the average emulsified particle size is determined by dynamic light scattering. For the case where the emulsified particle size exceeds 1,000 nm, the emulsified particle size is determined by a laser diffraction method because it is difficult to analyze the size by dynamic light scattering.

[0058] In another aspect, the fat composition of the present invention may be prepared to contain water in an amount of 0.5 to 2.0 times the amount of ascorbic acid. A dispersion stability may be improved by adjusting the water content.

[0059] In a preferred aspect, the dispersion stability may be further improved by adjusting the water content and containing glycerin.

[0060] As a method for adjusting the water content, a dehydration process may be performed in addition to the method using an ascorbic acid aqueous solution having a saturated solubility or higher as described in the present specification. The dehydration process is not limited as long as the content of water may be adjusted to a content specified in the present invention. A method may be exemplified in which water in a mixture of an oil phase and an aqueous phase that have been mixed and emulsified is distilled out with stirring, and various stirrers may be used. Examples of the stirring method include a method including using an inert gas for preventing oxidization and deterioration of fat, and distilling out water while performing bubbling agitation with the inert gas from the bottom of the container charged with a mixture of an oil phase and an aqueous phase that have been mixed and emulsified. For efficiently performing dehydration, a method of performing dehydration in a vacuum is preferable.

[0061] Examples of the method of dehydrating in a vacuum include a method including stirring under the condition of 50 to 130° C. and 0.5 to 50 Torr (0.067 kPa to 6.67 kPa), and adjusting the amount of water to be 0.5 to 2.0 times the amount of ascorbic acid.

[0062] The fat composition of the present invention may stably disperse ascorbic acid. Specifically, the composition may maintain a state in which ascorbic acid in fat is uniformly dispersed under conditions of heating and stirring. As an index of ability of stably dispersing ascorbic acid, the dispersion stability is evaluated herein by the presence or absence of precipitation after stirring at 60° C.

[0063] The fat composition of the present invention has an effect of enhancing the amount of taste itself, and further has an effect of reinforcing a rise of expression of taste, or persistence of taste without modifying original tastes such as salty taste, sour taste, pungent taste, and umami of food materials containing, particularly, taste materials, such as salty taste materials, sour taste materials, pungent taste materials, and umami materials which are characteristics of the ascorbic acid-containing fat composition. By improving dispersibility, this effect is kept high. This effect may be evaluated herein using an effect of enhancing salty taste as an index by preparing a taste enhancing fat having a predetermined content of ascorbic acid using an ascorbic acid-containing fat composition.

[0064] The fat composition of the present invention contains ascorbic acid and has favorable dispersion stability, and may exhibit favorable oxidative stability. For the effect related to the oxidative stability of the fat composition according to the present invention, a predetermined determination index may be obtained by a CDM test. Here, the CDM (Conductmetric Determination Method) may be used as an indicator for evaluation of oxidative stability of fat. A greater measurement value of the CDM indicates excellent oxidative stability. The CDM test may be performed using a dedicated test instrument (Rancimat). The specific measurement method of this method is also described in Examples. All the measurement methods are an acceleration test performed at a higher temperature than presumed use aspect.

[0065] The fat composition of the present invention may be adapted for a wide range of concentration and thus may be used for various foods. Examples of the food include seasonings such as dressing and mayonnaise, taste fillings and spreads such as pizza sauce, margarine and shortening, cooked foods such as retort curry, roux such as stew and curry, frozen foods such as fried chicken, dressed breads, processed meats such as sausage, fish pastes such as hanpen or foods prepared by cooking these products, rice confectioneries such as fried rice crackers, snack foods such as potato chips, corn snacks, and pretzels, frozen desserts, ice mix, and other confectioneries and Japanese confectioneries in which taste may be felt.

[0066] It is preferable that the fat composition of the present invention is contained in such a food at an amount of 100 to 5,000 mass ppm.

[0067] In a preferred aspect, the fat composition of the present invention may also be used for sauces, soups, beverages, fat for preventing sticking of cooked rice, frozen desserts, which have a small content of fat and are limited in their use.

[0068] Further, ascorbic acid may be efficiently stably dispersed in fat itself with a small amount of addition of the fat composition of the present invention, and the fat composition of the present invention may be used as a formulation of ascorbic acid with a high concentration. Since a special dispersion apparatus is not necessary for such use, fat containing ascorbic acid may be obtained as a formulation in various use of fat by adding the fat composition of the present invention.

[0069] The fat composition of the present invention may contain arbitrary components such as colorant, emulsifier, antioxidant, antifoaming agent, and flavor, as long as the effect of the present invention is not impaired. The total amount of the arbitrary components to be blended is preferably 5% by mass or less, more preferably 3% by mass or less, and most preferably 1% by mass or less in the fat composition of the present invention.

Examples

[0070] Hereinafter, the present invention will be described in more detail by way of Examples, but the spirit of the present invention is not limited by the following Examples. In Examples, % and part are based on weight.

(Method of Preparing Fat Composition)

[0071] An oil phase part was prepared in accordance with the formulation described in the column of the oil phase of formulation of fat composition in Tables. Similarly, an aqueous phase part was prepared in accordance with the formulation described in the column of aqueous phase of formulation of fat composition in Tables. These were prepared at 25° C. unless otherwise stated in the test examples. A low water content system was prepared at 60° C. In each case, the aqueous phase in which ascorbic acid was dissolved was prepared. Any of the aqueous phases was confirmed as substantially dissolved because no precipitate was visually observed by centrifuging 5 ml of the aqueous phase placed in a 20 ml centrifuge tube at 3,000 G for 1 minute.

[0072] A temperature of the oil phase part was set to be 25° C., and the aqueous phase part was blended while stirring by HOMOMIXER (TK homomixer MARKII: available from PRIMIX Corporation) at 8,000 rpm. Stirring was performed in this state for 10 minutes. The raw materials and additives used were as follows.

(Raw Materials and Additives)

[0073] As fat, “refined rapeseed oil”, palm olein “Palm Ace N”, and “refined palm oil” available from Fuji Oil Co., Ltd., were used. A melting point of the refined palm oil was 37° C.

[0074] As an oil-soluble emulsifier, polyglycerol condensed ricinoleic acid ester “Poem PR-100”, available from Riken Vitamin Co., Ltd. was used.

[0075] Ascorbic acid was available from Wako Pure Chemical Industries, Ltd.

[0076] Sodium hydroxide was available from Wako Pure Chemical Industries, Ltd.

[0077] Sodium ascorbate was available from DSM. As glycerin, “glycerin food additive”, available from Kishida Chemical Co., Ltd. was used.

(Method of Measuring pH of Aqueous Phase)

[0078] It was determined by diluting the aqueous phase with distilled water so that an amount of ascorbic acid as ascorbic acid equivalent is 10% by mass, and measuring with a pH meter.

(Method of Measuring Average Particle Size of Aqueous Phase)

[0079] An average particle size was determined by a dynamic light scattering method.

(Method of Evaluating Dispersion Stability of Ascorbic Acid-Containing Fat Composition)

[0080] It is an evaluation method that accelerates a deterioration of dispersibility due to moisture fluctuation and vibration. A sample, 10 g, was put into a 100 ml screw tube (manufactured by Nichiden Rika Glass Co., LTD.), and stirred (swirled) at 200 rpm using a shaking incubator (manufactured by Taitec corporation: BIO Shaker BR-43FL) in an open system at 60° C. for 7 days. After stirring, a condition after standing overnight was visually evaluated.

[0081] The evaluation criteria were “4: clear with no precipitate and crystal, 3: cloudy with no precipitate and crystal, 2: with precipitate and crystal, 1: Many precipitate and crystal were confirmed”. And, 3 points or more were considered to be excellent in dispersion stability and passed.

(Method of Evaluating Taste Enhancing Effect of Ascorbic Acid-Containing Fat Composition)

[0082] (1) Preparation Method of Taste Enhancing Fat from Ascorbic Acid-Containing Fat Composition

[0083] Using the ascorbic acid equivalent as a reference, the ascorbic acid-containing fat composition and rapeseed oil are mixed in accordance with the blending ratio in Tables such that the ascorbic acid content is 5 ppm as ascorbic acid equivalent, thus preparing a taste enhancing fat.

(2) Production Method of Non-Fried Potato Chips and Evaluation Method of Taste Enhancing Fat

[0084] Potatoes (Irish Cobbler potato produced in Hokkaido) were used. Each potato was sliced into 2 mm thick, and soaked in a 3% brine overnight. Moisture was wiped off with paper towel, and then moisture was removed by a microwave oven to prepare dried potato chips.

[0085] Subsequently, 20 parts of taste enhancing fat prepared in the above (1) was sprayed on 80 parts of the dried potato chips to prepare non-fried potato chips. Sensory evaluation was performed by 4 panelists, and the case where a strong salty taste was felt as compared to rapeseed oil used section (non-added section) was evaluated as “good” and the case where salty taste was felt equal or less than the rapeseed oil used section was evaluated as “poor”.

(Method of Evaluating Oxidative Stability of Ascorbic Acid-Containing Fat Composition)

[0086] A CDM value was determined and evaluated by a CDM value in accordance with “the stability test” of the CDM test of the method of the JOCS Standard Methods for the Analysis of Fats and Oils (2.5.1.2-1996) (definition: a clean air is supplied while a sample is heated at 120° C. in a reactor; a volatile decomposition product generated by oxidization is collected in water; and time until the inflection point where the conductivity of water changes rapidly is the CDM value).

[0087] A preparation was prepared by mixing an ascorbic acid-containing fat composition and a fat in a blending amount shown in Tables so that an amount of the ascorbic acid was 90 mass ppm as ascorbic acid equivalent.

[0088] A CDM value of the preparation was measured, and the preparation containing the ascorbic acid-containing fat composition showing an extension effect of 3 hr or more than the CDM value of the preparation without adding ascorbic acid-containing fat composition was regarded as acceptable.

(Study 1)

[0089] Fat compositions shown in the following Table were prepared according to the above (method of preparing fat composition). The following measurements and evaluations were carried out to the prepared fat compositions.

[0090] A pH of aqueous phase was measured according to the above (method of measuring pH of aqueous phase), and the results are shown in the following Table.

[0091] An average particle size was measured according to the above (method of measuring average particle size of aqueous phase), and the results are shown in the following Table.

[0092] A dispersion stability was evaluated according to the above (method of evaluating dispersion stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0093] A taste enhancing effect was evaluated according to the above (method of evaluating taste enhancing effect of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0094] An oxidative stability was evaluated according to the above (Method of evaluating oxidative stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

TABLE-US-00001 TABLE 1 Formulation of fat Comparative Comparative Comparative Comparative composition Example 1 Example 2 Example 1 Example 2 Example 3 Example 3 Example 4 Oil Rapeseed oil Part 99.8950 99.8890 99.8943 99.8941 99.8931 99.8925 99.8923 phase Palm olein Part Refined palm oil Part Oil-soluble Part 0.0450 0.0450 0.0450 0.0450 0.0450 0.0450 0.0450 emulsifier Aqueous Water Part 0.0480 0.0480 0.0480 0.0480 0.0480 0.0480 0.0480 phase Glycerin Part Ascorbic acid Part 0.0120 0.0180 0.0120 0.0120 0.0120 0.0120 0.0120 Sodium hydroxide Part 0.0000 0.0000 0.0007 0.0009 0.0019 0.0025 0.0027 Sodium ascorbate Part Total Part 100 100 100 100 100 100 100 Ascorbic acid 0.0120 0.0180 0.0120 0.0120 0.0120 0.0120 0.0120 equivalent pH of aqueous phase 2.2 2.2 3.6 4.0 4.8 6.0 7.2 Average particle 358 409 346 361 360 382 339 size Evaluation result 1 1 3 3 3 3 2 of dispersion stability Evaluation of taste enhancing effect Rapeseed oil Part 95.8333 97.2222 95.8333 95.8333 95.8333 95.8333 95.8333 Ascorbic acid- Part 4.1667 2.7778 4.1667 4.1667 4.1667 4.1667 4.1667 containing fat composition Ascorbic acid ppm 5.0 5.0 5.0 5.0 5.0 5.0 5.0 equivalent Evaluation result Good Good Good Good Good Bad Bad of taste enhancing effect Evaluation of oxidative stability Rapeseed oil Part 25 50 25 25 25 25 25 Palm olein Part Refined palm oil Part Ascorbic acid- Part 75 50 75 75 75 75 75 containing fat composition Ascorbic acid ppm 90.0 90.0 90.0 90.0 90.0 90.0 90.0 equivalent CDM value of hr 8.7 8.6 8.6 8.9 8.4 7.9 7.5 preparation CDM value hr 5.0 5.0 5.0 5.0 5.0 5.0 5.0 without additive Extension effect hr 3.7 3.6 3.6 3.9 3.4 2.9 2.5

(Evaluation Result)

[0095] The fat compositions of Examples in which the pH of the aqueous phase was adjusted to 2.5 to 5.3 did not generate precipitation before and after the test, and the dispersion stability was good. On the other hand, the Comparative Examples showed significantly reduced dispersion stability, and the dispersion stability was inferior.

[0096] The fat compositions of Examples had a taste enhancing effect. On the other hand, among the Comparative Examples, those having more than 5.3 of pH of the aqueous phase did not have a taste enhancing effect.

[0097] The fat compositions of Examples were excellent in the extension effect of the oxidative stability.

(Study 2)

[0098] Fat compositions shown in the following Table were prepared according to the above (method of preparing fat composition). The following measurements and evaluations were carried out to the prepared fat compositions.

[0099] A pH of aqueous phase was measured according to the above (method of measuring pH of aqueous phase), and the results are shown in the following Table.

[0100] An average particle size was measured according to the above (method of measuring average particle size of aqueous phase), and the results are shown in the following Table.

[0101] A dispersion stability was evaluated according to the above (method of evaluating dispersion stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0102] A taste enhancing effect was evaluated according to the above (method of evaluating taste enhancing effect of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0103] An oxidative stability was evaluated according to the above (Method of evaluating oxidative stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

TABLE-US-00002 TABLE 2 Formulation of fat Comparative Comparative composition Example 5 Example 4 Example 6 Example 5 Oil Rapeseed oil Part phase Palm olein Part 99.8950 99.8942 Refined palm oil Part 99.8950 99.8942 Oil-soluble Part 0.0450 0.0450 0.0450 0.0450 emulsifier Aqueous Water Part 0.0480 0.0480 0.0480 0.0480 phase Glycerin Part Ascorbic acid Part 0.0120 0.0120 0.0120 0.0120 Sodium hydroxide Part 0.0008 0.0008 Sodium ascorbate Part Total Part 100 100 100 100 Ascorbic acid 0.0120 0.0120 0.0120 0.0120 equivalent pH of aqueous phase 2.2 3.8 2.2 3.8 Average particle 483 496 359 401 size Evaluation result 1 3 1 3 of dispersion stability Evaluation of taste enhancing effect Rapeseed oil Part 95.8333 95.8333 95.8333 95.8333 Ascorbic acid- Part 4.1667 4.1667 4.1667 4.1667 containing fat composition Ascorbic acid ppm 5.0 5.0 5.0 5.0 equivalent Evaluation result Good Good Good Good of taste enhancing effect Evaluation of oxidative stability Rapeseed oil Part Palm olein Part 25 25 Refined palm oil Part 25 25 Ascorbic acid- Part 75 75 75 75 containing fat composition Ascorbic acid ppm 90.0 90.0 90.0 90.0 equivalent CDM value of hr 14.6 14.8 16.9 16.8 preparation CDM value without hr 11.0 11.0 13.3 13.3 additive Extension effect hr 3.6 3.8 3.6 3.5

(Evaluation Result)

[0104] The fat composition of Example in which the pH of the aqueous phase was adjusted to 2.5 to 5.3 did not generate precipitation before and after the test even when the fat composition was prepared from palm olein, and the dispersion stability was good. On the other hand, the Comparative Example showed significantly reduced dispersion stability, and the dispersion stability was inferior.

[0105] The fat composition of Example in which the pH of the aqueous phase was adjusted to 2.5 to 5.3 did not generate precipitation before and after the test even when the fat composition was prepared from refined palm oil which is necessary to heat before use due to more than 20° C. of the melting point, and the dispersion stability was good. On the other hand, the Comparative Example showed significantly reduced dispersion stability, and the dispersion stability was inferior.

[0106] The fat compositions of Examples had a taste enhancing effect and were excellent in the extension effect of the oxidative stability.

(Study 3)

[0107] Fat compositions shown in the following Table were prepared according to the above (method of preparing fat composition). The following measurements and evaluations were carried out to the prepared fat compositions.

[0108] A pH of aqueous phase was measured according to the above (method of measuring pH of aqueous phase), and the results are shown in the following Table.

[0109] An average particle size was measured according to the above (method of measuring average particle size of aqueous phase), and the results are shown in the following Table.

[0110] A dispersion stability was evaluated according to the above (method of evaluating dispersion stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0111] A taste enhancing effect was evaluated according to the above (method of evaluating taste enhancing effect of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0112] An oxidative stability was evaluated according to the above (Method of evaluating oxidative stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

TABLE-US-00003 TABLE 3 Formulation of fat Comparative Comparative composition Example 7 Example 6 Example 8 Example 7 Oil Rapeseed oil Part phase Palm olein Part 98.5800 98.5320 72.0000 71.8080 Refined palm oil Part Oil-soluble Part 0.30000 0.30000 12.0000 12.0000 emulsifier Aqueous Water Part 0.3200 0.3200 12.8000 12.8000 phase Glycerin Part Ascorbic acid Part 0.80000 0.80000 3.2000 3.2000 Sodium hydroxide Part 0.0480 0.1920 Sodium ascorbate Part Total Part 100 100 100 100 Ascorbic acid 0.8000 0.8000 3.2000 3.2000 equivalent pH of aqueous phase 2.2 3.7 2.2 3.8 Average particle 367 311 350 382 size Evaluation result 1 3 1 3 of dispersion stability Evaluation of taste enhancing effect Rapeseed oil Part 99.9375 99.9375 99.9843 99.9843 Ascorbic acid- Part 0.0625 0.0625 0.0157 0.0157 containing fat composition Ascorbic acid ppm 5.0 5.0 5.0 5.0 equivalent Evaluation result Good Good Good Good of taste enhancing effect Evaluation of oxidative stability Rapeseed oil Part 98.8750 98.8750 99.7187 99.7187 Palm olein Part Refined palm oil Part Ascorbic acid- Part 1.1250 1.1250 0.2813 0.2813 containing fat composition Ascorbic acid ppm 90.0 90.0 90.0 90.0 equivalent CDM value of hr 8.8 8.9 8.7 8.6 preparation CDM value without hr 5.0 5.0 5.0 5.0 additive Extension effect hr 3.8 3.9 3.7 3.6

(Evaluation Result)

[0113] The fat compositions of Examples in which the pH of the aqueous phase was adjusted to 2.5 to 5.3 did not generate precipitation before and after the test even when the fat composition was prepared with high concentration of ascorbic acid, and the dispersion stability was good. On the other hand, the Comparative Examples showed significantly reduced dispersion stability, and the dispersion stability was inferior.

[0114] The fat compositions of Examples had a taste enhancing effect and were excellent in the extension effect of the oxidative stability.

[0115] From the evaluation results, it is clear that the fat composition of the present invention may be applied in a wide concentration range.

(Study 4)

[0116] Fat compositions shown in the following Table were prepared according to the above (method of preparing fat composition). The following measurements and evaluations were carried out to the prepared fat compositions.

[0117] A pH of aqueous phase was measured according to the above (method of measuring pH of aqueous phase), and the results are shown in the following Table.

[0118] An average particle size was measured according to the above (method of measuring average particle size of aqueous phase), and the results are shown in the following Table.

[0119] A dispersion stability was evaluated according to the above (method of evaluating dispersion stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0120] A taste enhancing effect was evaluated according to the above (method of evaluating taste enhancing effect of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0121] An oxidative stability was evaluated according to the above (Method of evaluating oxidative stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

TABLE-US-00004 TABLE 4 Formulation of fat Comparative composition Example 8 Example 9 Example 10 Example 9 Oil Rapeseed oil Part 99.8926 99.8850 99.8860 99.8890 phase Palm olein Part Refined palm oil Part Oil-soluble Part 0.0450 0.0450 0.0450 0.0450 emulsifier Aqueous Water Part 0.0480 0.0480 0.0480 0.0480 phase Glycerin Part Ascorbic acid Part 0.0120 0.0120 0.0030 0.0000 Sodium hydroxide Part Sodium ascorbate Part 0.0024 0.0100 0.0180 0.0180 Total Part 100 100 100 100 Ascorbic acid 0.0141 0.0209 0.0190 0.0160 equivalent pH of aqueous phase 3.5 4.0 5.0 7.5 Average particle 538 560 380 482 size Evaluation result 3 3 3 1 of dispersion stability Evaluation of taste enhancing effect Rapeseed oil Part 96.4623 97.6065 97.3688 96.8755 Ascorbic acid- Part 3.5377 2.3935 2.6312 3.1245 containing fat composition Ascorbic acid ppm 5.0 5.0 5.0 5.0 equivalent Evaluation result Good Good Good Bad of taste enhancing effect Evaluation of oxidative stability Rapeseed oil Part 36.3225 56.9182 52.6388 43.7602 Palm olein Part Refined palm oil Part Ascorbic acid- Part 63.6775 43.0818 47.3612 56.2398 containing fat composition Ascorbic acid ppm 90.0 90.0 90.0 90.0 equivalent COM value of hr 8.5 8.4 8.5 7.7 preparation CDM value without hr 5.0 5.0 5.0 5.0 additive Extension effect hr 3.5 3.4 3.5 2.7

(Evaluation Result)

[0122] The fat compositions of Examples in which the pH of the aqueous phase was adjusted to 2.5 to 5.3 did not generate precipitation before and after the test even when the fat composition was prepared using sodium ascorbate, and the dispersion stability was good. On the other hand, the Comparative Examples showed significantly reduced dispersion stability, and the dispersion stability was inferior.

[0123] The fat compositions of Examples had a taste enhancing effect. On the other hand, among the Comparative Examples, those having more than 5.3 of pH of the aqueous phase did not have a taste enhancing effect.

[0124] The fat compositions of Examples were excellent in the extension effect of the oxidative stability.

[0125] By using sodium ascorbate, pH adjustment is easy and the melting rate is fast, so that the aqueous phase may be prepared quickly.

(Study 5)

[0126] Fat compositions shown in the following Table were prepared according to the above (method of preparing fat composition). The following measurements and evaluations were carried out to the prepared fat compositions.

[0127] A pH of aqueous phase was measured according to the above (method of measuring pH of aqueous phase), and the results are shown in the following Table.

[0128] An average particle size was measured according to the above (method of measuring average particle size of aqueous phase), and the results are shown in the following Table.

[0129] A dispersion stability was evaluated according to the above (method of evaluating dispersion stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0130] A taste enhancing effect was evaluated according to the above (method of evaluating taste enhancing effect of ascorbic acid-containing fat composition), and the results are shown in the following Table.

[0131] An oxidative stability was evaluated according to the above (Method of evaluating oxidative stability of ascorbic acid-containing fat composition), and the results are shown in the following Table.

TABLE-US-00005 TABLE 5 Formulation of fat Comparative Comparative composition Example 10 Example 11 Example 12 Example 11 Oil Rapeseed oil Part 99.8927 99.8920 99.8883 99.8875 phase Palm olein Part Refined palm oil Part Oil-soluble Part 0.0450 0.0450 0.0450 0.0450 emulsifier Aqueous Water Part 0.0143 0.0143 0.0168 0.0168 phase Glycerin Part 0.0360 0.0360 0.0360 0.0360 Ascorbic acid Part 0.0120 0.0120 0.0120 0.0120 Sodium hydroxide Part 0.0007 0.0019 0.0027 Sodium ascorbate Part Total Part 100 100 100 100 Ascorbic acid 0.0120 0.0120 0.0120 0.0120 equivalent pH of aqueous phase 2.2 3.7 4.8 5.5 Average particle 293 409 330 351 size Evaluation result 1 4 4 2 of dispersion stability Evaluation of taste enhancing effect Rapeseed oil Part 95.8333 95.8333 95.8333 95.8333 Ascorbic acid- Part 4.1667 4.1667 4.1667 4.1667 containing fat composition Ascorbic acid ppm 5.0 5.0 5.0 5.0 equivalent Evaluation result Good Good Good Bad of taste enhancing effect Evaluation of oxidative stability Rapeseed oil Part 25 25 25 25 Palm olein Part Refined palm oil Part Ascorbic acid- Part 75 75 75 75 containing fat composition Ascorbic acid ppm 90.0 90.0 90.0 90.0 equivalent COM value of hr 8.8 9.0 8.6 8.1 preparation CDM value without hr 5.0 5.0 5.0 5.0 additive Extension effect hr 3.8 4.0 3.6 3.1

(Evaluation Result)

[0132] The fat compositions of Examples in which the pH of the aqueous phase was adjusted to 2.5 to 5.3 did not generate precipitation before and after the test even when the fat composition was prepared with low water content, i.e. water content was adjusted to 0.5 to 2.0 times a content of the ascorbic acid, and the dispersion stability was good. On the other hand, the Comparative Examples showed significantly reduced dispersion stability, and the dispersion stability was inferior.

[0133] The fat compositions of Examples had a taste enhancing effect. On the other hand, among the Comparative Examples, those having more than 5.3 of pH of the aqueous phase did not have a taste enhancing effect.

[0134] The fat compositions of Examples were excellent in the extension effect of the oxidative stability.

[0135] The fat composition with a low water content using glycerin showed greatly reduced turbidity during stirring at 60° C., and had more excellent dispersion stability.

[0136] The rapeseed oil preparation product obtained by diluting the fat composition of the present invention also had an excellent extension effect of oxidative stability.

[0137] The present invention enables to obtain food containing ascorbic acid, and having improved oxidative stability and enhanced taste, by adding a fat composition of the present invention in which the ascorbic acid concentration range is optimally adjusted to food having any fat content.

INDUSTRIAL APPLICABILITY

[0138] The present invention enables to provide ascorbic acid-containing fat that has an improved content of ascorbic acid in ascorbic acid-containing fat, and enables to use in various foods.