USE OF PENICILLIUM SCLEROTIORUM IN PREPARATION OF PLANT GROWTH REGULATOR OR INDUCER

Abstract

Provided is use of Penicillium sclerotiorum SCAUMCX01, under accession number GDMCC No. 60249, in the preparation of plant growth regulators or inducers. The plant growth regulators or inducers containing Penicillium sclerotiorum, Penicillium sclerotiorum spores, a culture solution thereof and an extract of the culture solution have the effects of promoting the early germination of seeds, promoting the growth of the underground roots of crops and the branching of above-ground parts of crops, improving the bioavailability of crops, delaying the aging progress of crops, and enhancing the growth of crops.

Claims

1. Use of Penicillium sclerotiorum in the preparation of plant growth regulators or inducers, characterized in that said Penicillium sclerotiorum is Penicillium sclerotiorum (from Portulaca oleracea L.) SCAUMCX01, deposited on Oct. 11, 2017 at Guangdong Microbial Culture Collection Center, 5th floor, 59 blg, No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, under accession number GDMCC No. 60249.

2. The use of Penicillium sclerotiorum in the preparation of plant growth regulators or inducer according to claim 1, characterized in that: the plant is a crop; the effective concentration of Penicillium sclerotiorum is from 0.0001 mg/ml to 10 mg/ml.

3. The use of Penicillium sclerotiorum in the preparation of plant growth regulators or inducer according to claim 2, characterized in that: the plant is rice, corn, wheat or Arabidopsis.

4. The use of Penicillium sclerotiorum in the preparation of plant growth regulators or inducer according to claim 1, characterized in that: the plant growth regulator or inducer is the one that promotes the seed germination of the plant, promotes the root growth of the plant, promotes the lateral root increase of the plant, promotes the late stage growth of the plant, improves the bioavailability of the plant, and/or delays the aging progress of the plant.

5. The use of Penicillium sclerotiorum in the preparation of plant growth regulators or inducers according to claim 1, characterized in that: the major components of the plant growth regulators or inducers are Penicillium sclerotiorum, Penicillium sclerotiorum spores, the culture solution of Penicillium sclerotiorum, the extract of the culture solution of Penicillium sclerotiorum, or the secondary metabolites produced by Penicillium sclerotiorum; wherein the culture solution of Penicillium sclerotiorum is obtained by the following method: Penicillium sclerotiorum is inoculated into a culture medium, and cultivated for 1-30 days at 100 r/min and at 28° C. so as to obtain the culture solution of Penicillium sclerotiorum; wherein the medium is PDB medium.

6. Use of Penicillium sclerotiorum as plant growth regulators or inducers, characterized in that said Penicillium sclerotiorum is Penicillium sclerotiorum (from Portulaca oleracea L.) SCAUMCX01, deposited on Oct. 11, 2017 at Guangdong Microbial Culture Collection Center, 5th floor, 59 blg, No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, under accession number GDMCC No. 60249.

7. Use of Penicillium sclerotiorum as plant growth regulators or inducers according to claim 6, characterized in that the culture solution of Penicillium sclerotiorum is irrigated to the plant root, or the culture solution of Penicillium sclerotiorum is sprayed on the plant, or the seeds are soaked into the culture solution of Penicillium sclerotiorum; wherein the culture solution of Penicillium sclerotiorum is obtained by any one of the following methods: (1) Penicillium sclerotiorum is inoculated into a culture medium, and cultivated for 1-30 days at 100 r/min and at 28° C. so as to obtain the culture solution of Penicillium sclerotiorum; (2) Penicillium sclerotiorum is inoculated into a culture medium, and cultivated for 1-30 days at 100 r/min and at 28° C., and then filtered with gauze so as to obtain the culture solution of Penicillium sclerotiorum; wherein the culture medium in methods (1) and (2) is PDB medium.

8. Use of Penicillium sclerotiorum as plant growth regulators or inducers according to claim 6, characterized in that: the plant is a crop; the concentration of the culture solution of Penicillium sclerotiorum is from 0.0001 mg/ml to 10 mg/ml.

9. Use of Penicillium sclerotiorum in the preparation of drug formulations or biological formulations for improving microorganisms in soil, characterized in that: said Penicillium sclerotiorum is Penicillium sclerotiorum (from Portulaca oleracea L.) SCAUMCX01, deposited on Oct. 11, 2017 at Guangdong Microbial Culture Collection Center, 5th floor, 59 blg, No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, under accession number GDMCC No. 60249. wherein the effective dose of Penicillium sclerotiorum is 0.0005-0.5 wt %.

10. The use of Penicillium sclerotiorum in the preparation of plant growth regulators or inducers according to any one of claims 1 to 5, characterized in that the plant growth regulator or inducer is irrigated to the plant root, or the plant growth regulator or inducer is sprayed on the plant, or the plant seeds are soaked into the plant growth regulator or inducer.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0041] FIG. 1 is a diagram showing the effect of Penicillium sclerotiorum on corn growth; wherein A) is the result photographed on the day of treating corns with the culture solution or the culture solution containing Penicillium sclerotiorum; B) is the result photographed 1 month after treating corns with the culture solution or the culture solution containing Penicillium sclerotiorum.

[0042] FIG. 2 is diagram showing the effect of Penicillium sclerotiorum on the germination of rice seeds; wherein, A) is the result photographed 4 days after treating rice seeds with the pure culture solution or the culture solution containing Penicillium sclerotiorum; B) is the statistical result of germination rate after treating rice seeds with the pure culture solution or the culture solution containing Penicillium sclerotiorum.

[0043] FIG. 3 is a diagram showing the effect of Penicillium sclerotiorum on rice growth; wherein, A) is the result photographed on the day of treating rice with the culture solution or the culture solution containing Penicillium sclerotiorum; B) is the result photographed 9 days after treating rice with the pure culture solution or the culture solution containing Penicillium sclerotiorum; and C) is the result photographed 22 days after treating rice with the pure culture solution or the culture solution containing Penicillium sclerotiorum.

[0044] FIG. 4 is a diagram showing the effect of Penicillium sclerotiorum on the growth of rice underground roots; wherein, A) is the rice underground root result photographed 22 days after treating rice with the culture solution or the culture solution containing Penicillium sclerotiorum; B) is an average weight statistical plot of rice underground roots.

[0045] FIG. 5 is a diagram showing the effect of Penicillium sclerotiorum on the growth of Arabidopsis.

DETAILED DESCRIPTION OF THE INVENTION

[0046] Hereinafter, the present invention will be further described in detail with reference to the examples, but the embodiments of the present invention are not limited thereto. It should be understood that the examples described in this description are merely for illustrating the present invention, and are not intended to limit the present invention. Unless otherwise specified, the agents and raw materials used in the present invention are commercially available.

[0047] The Penicillium sclerotiorum in the present invention is obtained from Penicillium sclerotiorum (from Portulaca oleracea L.) SCAUMCX01, deposited on Oct. 11, 2017 at Guangdong Microbial Culture Collection Center, 5th floor, 59 blg, No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, under accession number GDMCC No. 60249.

[0048] The culture medium of Penicillium sclerotiorum in the present invention can be a solid or liquid medium made from the crop, such as rice, rice hull, wheat, wheat hull, potatoes or the like, as raw materials, for example, commercial available Czapek-Dox medium, malt extract medium, potato medium, and the like; preferably a culture medium made from potato as a raw material. The culture medium in the examples is liquid Czapek-Dox medium (PDB), which is purchased from Guangdong Huankai Microbial Sci. & Tech. Co., Ltd.

[0049] In the present invention, the culture solution of Penicillium sclerotiorum is obtained by directly cultivating Penicillium sclerotiorum (the cultivated Penicillium sclerotiorum is directly grown on the culture medium). The extract of the culture solution of Penicillium sclerotiorum is obtained by extracting with an organic solvent such as ethyl acetate, chloroform, n-butanol, etc., the extract liquid can be further concentrated and dried to obtain a concrete, the concrete can be further formulated into drug formulation by adding water or a certain amount of emulsion agent, the cultivation time of the culture solution of Penicillium sclerotiorum is 1-30 days (preferably 14 days). The culture solution of Penicillium sclerotiorum can be filtered with gauze so as to remove the large bacterial impurity blocks. In the examples, the method for the preparation of the culture solution containing Penicillium sclerotiorum comprises: Penicillium sclerotiorum is inoculated into the PDB medium, and cultivated for 10 days at the rotation speed of the shaker of 100 r/min, at 28° C. so as to obtain the culture solution containing Penicillium sclerotiorum.

Example 1

[0050] Designed experiment: Corns (B73) (Yan, J. et al. Accumulation of 5-hydroxynorvaline in maize (Zea mays) leaves is induced by insect feeding and abiotic stress, J. Exp. 2015, 66, 593-602) are cultivated to have 3-4 leaves by a conventional method, then 3 pots of plants are used as blank controls (control1, control2, control3), 3 pots of plants are used as test groups (test1, test2, test3); wherein 150 ml of the culture solution containing Penicillium sclerotiorum is directly added into the test groups (3 strains of plants in total, 50 ml per strain of plant), and 150 ml of PDB culture solution is directly added into the blank groups (3 strains of plants in total, 50 ml per stain of plant), and photographs are taken on the same day. Subsequently, water is added in regular amount at regular time every other day to ensure the normal growth of corns. Photographs are taken after 1 month, and used for comparison.

[0051] As shown in FIG. 1, it is found that after the culture solution containing Penicillium sclerotiorum is added, the corns grow better, have strong stem, and the leaves thereof at the bottom are aged slowly.

Example 2

[0052] 20 rice seeds (Oryza. Sativa L. spp. japonica) (Yan J. et al. The Tyrosine Aminomutase TAM1 Is Required for beta-Tyrosine Biosynthesis in Rice, Plant Cell, 2015, 27, 1265-1278) are respectively placed in blank culture solution (namely, PDB culture solution) and the culture solution containing Penicillium sclerotiorum, and the germination rate of the seeds is observed after 4 days.

[0053] The photograph result is shown in FIG. 2(A), and it is obtained from the statistical result of data (FIG. 2(B)) that the culture solution containing Penicillium sclerotiorum can significantly increase the germination rate of rice seeds.

Example 3

[0054] When rices (Oryza. Sativa L. spp. japonica) (Yan J. et al. The Tyrosine Aminomutase TAM1 Is Required for beta-Tyrosine Biosynthesis in Rice, Plant Cell, 2015, 27, 1265-1278) are grown to have 4-5 leaves, 50 ml of the culture solution containing Penicillium sclerotiorum and the blank culture solution (namely, PDB culture solution) are respectively added to each plant, repeated 3 times, labeled as control group (control1-3) and test group (test1-3) respectively. Photographs are taken on the same day (Jul. 29, 2017), then photographs are taken on Aug. 7, 2017, and Aug. 20, 2017, respectively, and used for comparison.

[0055] As shown in FIG. 3, it can be easily seen that after the culture medium containing Penicillium sclerotiorum is used, the rices grow stronger and the leaves turn yellow less. The growth of the underground roots is further analyzed, and as shown in FIG. 4, it can be easily seen that the roots of the test groups applied with Penicillium sclerotiorum develop more and grow more. The dry weight statistical result of roots (comparison of Aug. 20, 2017 with the day of treatment) also provides the consistent result—the root weight of the Penicillium sclerotiorum test group increases significantly.

Example 4

[0056] Designed experiment: when Arabidopsis (Arabidopsis thaliana) (http://signal. salk. edu/) is grown to have 4 leaves, 3 pots of Arabidopsis under the same grow status are selected, divided into 3 groups, and then the test group is directly added with 50 ml of the culture solution containing Penicillium sclerotiorum (labeled as test group A), and the blank group is directly added with 50 ml of PDB culture solution (labeled as culture solution-1B) or pure water (labeled as water 1C), and photographs are taken on the same day; wherein, 1 pot of plant is used as water blank control, 1 pot of plant is used as culture solution blank control, and 1 pot of plant is used as test group of culture solution containing Penicillium sclerotiorum. Subsequently, water is added in regular amount at regular time every other day to ensure the normal growth of Arabidopsis, and photographs are taken after 10 days and used for comparison (labeled as test group A1, culture solution-1B1, water-1C1). The above experiments are repeated on the same day by adding 50 ml of culture solution containing Penicillium sclerotiorum, the culture solution and pure water respectively, and photographs are taken after 10 days (test group A2, culture solution-1B2, water-1C2);

[0057] Some of the results are shown in FIG. 5. It was found that after the culture solution containing Penicillium sclerotiorum is added, the Arabidopsis grow better, has larger leaves and more leaves.

[0058] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations and simplifications made without departing from the spirit and principle of the present invention, which should be equivalent replacement manners, all fall in the scope of protection of the present invention.