Polymeric paste compositions for drug delivery

Abstract

This invention provides compositions for controlled localized release of one or more drugs within a subject. More particularly, described herein are compositions comprising a hydrophobic water-insoluble polymer, a low molecular weight biocompatible glycol, and one or more drugs. The compositions described herein may also optionally include a di-block copolymer and/or a swelling agent.

Claims

1. A composition consisting of a mixture of the following components: (a) a hydrophobic water-insoluble polymer having an inherent viscosity (IV) of about 0.15 to about 0.5 dL/g; (b) a low molecular weight biocompatible glycol; with a molecular weight at or below 1,450 Daltons; and (c) one or more drug compounds or pharmaceutically acceptable salt, solvate or solvate of the salt thereof, wherein the components (a) and (b) do not form covalent bonds with each other, and the ratio of the low molecular weight biocompatible glycol to the hydrophobic water-insoluble polymer is between about 70%:30% and about 40%:60%.

2. The composition of claim 1, wherein the hydrophobic water-insoluble polymer having an inherent viscosity (IV) of about 0.15 to about 0.5 dL/g is polylactic-co-glycolic acid (PLGA).

3. The composition of claim 2, wherein the PLGA has a ratio of lactic acid (LA):glycolic acid (GA) at or below 75:25.

4. The composition of claim 1, wherein the hydrophobic water-insoluble polymer has an inherent viscosity (IV) of about 0.15 to about 0.3 dL/g.

5. The composition of claim 1, wherein the hydrophobic water-insoluble polymer has an inherent viscosity (IV) of about 0.15 to about 0.2.5 dL/g.

6. The composition of claim 1, wherein the low molecular weight biocompatible glycol has a molecular weight between about 76 Daltons and about 1,450 Daltons.

7. The composition of claim 1, wherein the low molecular weight biocompatible glycol is selected from Polyethylene glycol (PEG), methoxypolyethylene glycol (mePEG) and propylene glycol.

8. The composition of claim 1, wherein the low molecular weight biocompatible glycol is selected from PEG and mePEG.

9. The composition of claim 8, wherein the PEG or mePEG has an average molecular weight of between 300 Daltons and 1,450 Daltons.

10. The composition of claim 1, wherein the hydrophobic water-insoluble polymer having an inherent viscosity (IV) of about 0.15 to about 0.5 dL/g is PLGA having an LA:GA ratio of 50:50 and the low molecular weight biocompatible glycol is PEG or mePEG with a molecular weight of about 300 Daltons to about 1,450 Daltons.

11. The composition of claim 10, wherein the ratio of PEG or mePEG to PLGA is between about 60%40% and about 40%:60%.

12. The composition of claim 11, wherein the ratio of PEG or mePEG to PLGA is between about 60%:40% and about 50%:50%.

13. The composition of claim 1, wherein the low molecular weight biocompatible glycol is PEG 300.

14. The composition of claim 1, wherein the one or more drug compounds or pharmaceutically acceptable salt, solvate or solvate of the salt thereof is selected from one or more of the following categories: anti-cancer drugs; anti-inflammatory agents; anti-bacterial; anti-fibrotic; anesthetic; and analgesic.

15. The composition of claim 1, wherein the one or more drug compounds or pharmaceutically acceptable salt, solvate or solvate of the salt thereof is hydrophobic.

16. The composition of claim 1, wherein the one or more drug compounds or pharmaceutically acceptable salt, solvate or solvate of the salt thereof is hydrophilic.

17. The composition of claim 14, wherein the anti-cancer drug is selected from one or more of the following: Actinomycin; All-trans retinoic acid; Azacitidine; Azathioprine; Bleomycin; Bortezomib; Carboplatin; Capecitabine; Cisplatin; Chlorambucil; Cyclophosphamide; Cytarabine; Daunorubicin; Docetaxel; Doxifluridine; Doxorubicin; Epirubicin; Epothilone; Etoposide; Fluorouracil; Gemcitabine; Hydroxyurea; Idarubicin; Imatinib; Irinotecan; Mechlorethamine; Mercaptopurine; Methotrexate; Mitoxantrone; Oxaliplatin; Paclitaxel; Pemetrexed; Teniposide; Tioguanine; Topotecan; Valrubicin; Vemurafenib; Vinblastine; Vincristine; Vindesine; and Vinorelbine.

18. The composition of claim 14, wherein the anesthetic drug is a local anesthetic selected from one or more of the following: Procaine; Benzocaine; Chloroprocaine; Cocaine; Cyclomethycaine; Dimethocaine/Larocaine; Piperocaine; Propoxycaine; Procaine/Novocaine; Proparacaine; Tetracaine/Amethocaine; Articaine; Bupivacaine; Cinchocaine/Dibucaine; Etidocaine; Levobupivacaine; Lidocaine/Lignocaine/Xylocaine; Mepivacaine; Prilocaine; Ropivacaine; and Trimecaine.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 shows the viscosity of polymeric pastes with different weight ratios of PLGA, PEG and diblock copolymer.

(2) FIG. 2 shows the release of PEG 300™ from several polymeric paste mixtures.

(3) FIG. 3 shows the release of docetaxel at 4%, bicalutamide at 4%, and VPC-27 at 10% (A) and 4% (B) from PEG:PLGA (50:50) polymeric pastes.

(4) FIG. 4 shows the release of docetaxel (A), bicalutamide (B), and VPC-27 (C) from PEG:PLGA:Diblock polymeric pastes (57:37:13 or 50:24:26).

(5) FIG. 5 shows the release of docetaxel at 0.25%, bicalutamide at 4%, and VPC-27 at 4% from PEG:PLGA:Diblock polymeric pastes (57:37:13 w/w %).

(6) FIG. 6 shows the effect of varying PLGA:Diblock ratios (A) 43%:7%, (B) 37%:13%, (C) 31%:19% (D) 25%:25% on the release rates of docetaxel (0.5%), bicalutamide (4%), and VPC-27 (4%) from PEG:PLGA:Diblock pastes.

(7) FIG. 7 shows the release of docetaxel (A), bicalutamide (B), and VPC-27 (C) from PEG:PLGA polymeric pastes (63:37 or 76:24).

(8) FIG. 8 shows the release of docetaxel (1%), bicalutamide (4%), and VPC-27 (4%) from PEG:PLGA polymeric pastes (50:50 (A) or 55:45 (B) or 60:40 (C)).

(9) FIG. 9 shows the release of rapamycin (1%), docetaxel (1%), and VPC-27 (4%) from PEG:PLGA:Diblock polymeric pastes (50:37:13 w/w %).

(10) FIG. 10 shows the release of Cephalexin (A) 2% and (B) 4%, 6%, 8% and 10% from PEG:PLGA:Diblock polymeric paste (50:37:13 w/w %).

(11) FIG. 11 shows the release of lidocaine from various paste shapes (cylinder, crescent and hemisphere (A)) and at different lidocaine concentrations (2%, 4%, 6%, 8% and 10% (B)) from PEG:PLGA:Diblock polymeric paste (50:37:13 w/w %).

(12) FIG. 12 shows the release of 1% docetaxel (A), 4% Enzalutamide (B), or 4% VPC-27 (C) from PEG:PLGA:Diblock polymeric pastes (63:37 w/w % or 50:37:13 w/w %).

(13) FIG. 13 shows the solubilization of docetaxel, bicalutamide, or VPC-27 by diblock copolymer (molecular weight 3333, PLLA 40%, MePEG 2000 60%).

(14) FIG. 14 shows the release of lidocaine (10%) and desoximetasone (1%) from PEG:PLGA:Diblock polymeric paste (50:37:13 w/w %).

(15) FIG. 15 shows the release of lidocaine (8%) from PEG:PLGA pastes (50:50, 55:45 and 60:40).

(16) FIG. 16 shows the release of sunitinib (1%) from PEG:PLGA:Diblock polymeric paste (50:37:13 w/w %).

(17) FIG. 17 shows the release of Tamsulosin (2%) from PEG:PLGA:Diblock polymeric paste (50:37:13 w/w %).

(18) FIG. 18 shows the release of lidocaine (8%), cephalexin (2%), and ibuprofen (5%) from PEG:PLGA:Diblock polymeric paste (50:37:13 w/w %).

(19) FIG. 19 shows the effect of drug-loaded (i.e. docetaxel (1%), bicalutamide (1%), and VPC-27 (4%)) PEG:PLGA:Diblock polymeric paste (50:37:13 w/w %) versus control paste (i.e. no drug) on mouse serum PSA (A) and absolute tumour size (B) as a representation of human prostate cancer tumors (ST-PC3) in mice. A study to determine the effect of variable concentrations of docetaxel.

(20) FIG. 20 shows the effect of varied amounts of Docetaxel (i.e. 0%, 0.25%, 0.5% and 1%) drug-loaded (i.e. Bicalutamide (4%), and VPC-27 (4%) PEG:PLGA:Diblock polymeric paste (50:37:13 w/w %) on mouse serum PSA (A) and absolute tumour size (B) as a representation of human prostate cancer tumors in mice.

(21) FIG. 21 shows serum PSA concentrations (A) and tumour volume in cm.sup.3 (B) after intratumoral injection of drug-loaded pastes (1% Docetaxel and 4% Bicalutamide; 1% Docetaxel, 4% Bicalutamide and 4% VPC-27; 1% Docetaxel; and 1% Docetaxel and 4% VPC-27).

(22) FIG. 22 shows the systemic absorption of lidocaine after local injection of lidocaine pastes (in PEG/PLGA 50/50) subcutaneously in rats at different doses of lidocaine paste (i.e. 23 mg/kg; 29 mg/kg; 36 mg/kg; 40 mg/kg; and 45 mg/kg) as a measure of serum concentration observed over time (A) and as a semi logarithmic plot (B).

(23) FIG. 23 shows the water absorption of pastes containing a swelling agent (i.e. 2% sodium hyaluronate (SH)) and variable amounts of diblock copolymer (i.e. 10%, 20%, 30% and 40%) as compared to no SH and no diblock.

(24) FIG. 24 shows the release of 5% Gemcitabine from pastes with (i.e. 2% sodium hyaluronate (SH)) and without swelling agent and with and without diblock copolymer. The pastes had a high PEG 300™ content (i.e. 53%, 58% and 76%).

(25) FIG. 25 shows the urinary excretion after injection of 5% gemcitabine pastes into pig kidney pelvis (A) and serum gemcitabine concentrations after injection of 5% gemcitabine pastes (68% PEG: 30% PLGA) into pig kidney pelvis (B) to show systemic absorption of gemcitabine paste containing a swelling agent (2% SH) in three pigs (administration of 1.5 mL of a 5% gemcitabine paste into kidney pelvis using 5 F ureteral catheter).

DETAILED DESCRIPTION OF THE INVENTION

(26) In embodiments of the invention hydrophobic water-insoluble polymers are used to control the consistency of biocompatible polymer pastes and subsequent release of a variety of drugs therefrom.

(27) Inherent Viscosity (IV) is a viscometric method for measuring molecular size. IV is based on the flow time of a polymer solution through a narrow capillary relative to the flow time of the pure solvent through the capillary. The units of IV are typically reported in deciliters per gram (dL/g). IV is simple and inexpensive to obtain and reproducible. Gel Permeation Chromatography (GPC) may be used as a chromatographic method for measuring molecular size. The molecular size can be expressed as molecular weight (MW) in Daltons obtained from calibration with a standard polymer (for example, polystyrene standards in chloroform). The molecular weight of styrene is 104 Daltons and standards of known polystyrene are readily available. MWs obtained by GPC are very method-dependent and are less reproducible between laboratories. Alternatively, molecular weight may be measured by size exclusion chromatography (SEC), high temperature gel permeation chromatography (HT-GPC) or mass spectrometry (MALDI TOF-MS).

(28) The hydrophobic water-insoluble polymers may be a polyester. The hydrophobic water-insoluble polymers may be a polylactic-co-glycolic acid (PLGA), wherein, the ratio of LA:GA is equal to or below 75:25. The ratio of LA:GA may be about 50:50. Durect Corporation™ who supplied the PLGA used in these experiments graph inherent viscocity (IV) in dL/g in hexafluoroisopropanol (HFIP) against molecular weight in Daltons for their 50:50 and 65:35 LA:GA polymers. Similarly, when Durect™ calculated the IV values in dL/g for 75:25 PLGA and 85:15 PLGA, chloroform, was used as the solvent. The relationship between IV and molecular weight in Daltons is different depending on the ratio of LA:GA. As described herein an inherent viscocity of between 0.15 to 0.25 dL/g is an optional range, but an IV in the range 0.25-0.5 dL/g would also be suitable. Alternatively, the range may be between about 0.15 dL/g and about 0.5 dL/g.

(29) Using a 50:50 PLGA a range of 0.15 to 0.25 dL/g is approximately equivalent to a range of about 4,300 Daltons to about 6,700 Daltons and a range of 0.25 to 0.5 dL/g is approximately equivalent to a range of about 6,700 Daltons to about 26,600 Daltons. Using a 65:35 PLGA a range of 0.15 to 0.25 dL/g is approximately equivalent to a range of about 6,500 Daltons to about 14,200 Daltons and a range of 0.25 to 0.5 dL/g is approximately equivalent to a range of about 14,200 Daltons to about 39,000 Daltons. The broader range of 0.15 to 0.5 dL/g is equivalent to about 4,300 Daltons to about 26,600 daltons for 50:50 PLGA and about 6,500 Daltons to about 39,000 daltons for 65:35 PLGA. Accordingly, the Dalton range for PLGA may be anywhere between 4,300 and about 39,000. Alternatively, the Dalton range for PLGA may be anywhere between 4,300 and about 40,000 or higher if using 75:25 (i.e. up to a molecular weight of 56,500 Dalton). For the 50:50, 65:35 and 75:25 LA:GA polymers, an IV of 0.5 g/dL approximately corresponds to molecular weights of 26,600, 39,000, and 56,500. As tested the Durect™ 50:50 having an IV of 0.25 dL/g is about 6,700 Daltons, Durect™ 75:25 having an IV of 0.47 dL/g is about 55,000 Daltons and Durect™ 85:15 having an IV of 0.55 dL/g to 0.75 dL/g is in the range of about 76,000 Daltons to about 117,000 Daltons.

(30) Calculations of IV to Dalton's provided by Durect Corporation™ are as follows (for each ratio of LA:GA). For 50:50 an IV of 0.25 dL/g is about 6.700 Daltons, an IV of 0.35 dL/g is about 12,900, Daltons, an IV of 0.45 dL/g is about 21,100, an IV of 0.55 dL/g is about 31,100 Daltons and an IV of 0.65 dL/g is about 43.500 Daltons. For 65:35 an IV of 0.15 dL/g is about 6,500 Daltons, an IV of 0.25 dL/g is about 14,200 Daltons, an IV of 0.35 dL/g is about 23,700 Daltons, an IV of 0.45 dL/g is about 34,600 Daltons, an IV of 0.55 dL/g is about 47,000 Daltons and an IV of 0.65 dL/g is about 60,500 Daltons. For 75:25 an IV of 0.15 dL/g is about 11,200 Daltons, an IV of 0.25/0.3 dL/g is about 23,800 Daltons, an IV of 0.35/0.4 dL/g is about 39,000 Daltons, an IV of 0.45/0.5 dL/g is about 56,500 Daltons and an IV of 0.55/0.6 dL/g is about 76,000 Daltons.

(31) Of particular interest are PLGA pastes having a ratio of LA:GA of 50:50 with an IV of between 0.15 dL/g to 0.25 dL/g (i.e. molecular weights of between 4,300 Daltons to 6,700 Daltons). However, PLGA pastes having a ratio of LA:GA of 50:50 with an IV of 0.25 dL/g to 0.5 dL/g (i.e. a molecular weight of about 6,700 to about 26,600 Daltons) is also useful.

(32) The PLGA polymer molecular weight may be reported as inherent viscocity (IV)) may be IV=0.15-0.5 dL/g. The PLGA polymer IV may be <0.3 dL/g. The PLGA polymer density may lie between 0.15-0.25 dL/g. Low molecular weight versions of PLGA with a 50:50 ratio of LA:GA and an inherent viscosity under 0.3 dL/g may be rendered fully miscible with a low molecular weight biocompatible glycol using mild heating to form either a viscous or fluid paste at room temperature. For high viscosity pastes, the 50:50 ratio PLGA materials with an inherent viscosity up to 0.5 dL/g may be used with poly ethylene glycol (PEG). PEG or mePEG with a molecular weight below 1450 may be used in these applications. The low molecular weight biocompatible glycol may have a molecular weight between about 76 and about 1450. The PEG or mePEG may have an average molecular weight of between 300 and 1450.

(33) Low molecular weight biocompatible glycol may be used to fluidize PLGA to a paste and set to an implant. Examples of a low molecular weight biocompatible glycol may include PEG, mePEG and propylene glycol. A PEG-based glycol (i.e. mePEG or PEG) may have a molecular weight of up to 1450. Alternatively, the PEG-based excipient may have a molecular weight 900. In a further alternative, a PEG-based excipient may have a molecular weight of about 300. PEG 300™ is biocompatible and is directly cleared via the kidneys without liver or other degradation required.

(34) PLGA:PEG pastes may be loaded with a variety of drugs and allow for controlled release of the loaded drug(s) over periods of approximately 1-2 months. Low molecular weight diblock copolymers may also be optionally incorporated without phase separation into the PLGA:PEG compositions with only minor changes in viscosity of the total composition. The presence of diblock copolymers may allow further control (acceleration) of drug release from the polymer matrix so that certain drugs that release slowly may be released more rapidly.

(35) Diblock copolymers may consist of two different types of monomers. The monomers may be hydrophobic. The monomers may be hydrophilic. The diblock copolymer may have one hydrophobic monomer and one hydrophilic monomer. The diblock copolymer may be amphiphilic. The hydrophilic monomer for example, may be PEG or MePEG. The hydrophobic monomer for example, may be PLGA, PLA, PLLA or PCL. TABLE 1 below provides a range of compositions that were made and tested to determine their characteristics and useful features.

(36) TABLE 1 provides examples of various polymer formulations as tested.

(37) TABLE-US-00001 Optional Injectability Set time PLGA IV or Diblock Form at (needle in water alternative % Glycol % Copolymer % injection size/force) starts 0.15-0.25 25 PEG 300 ™ 75 Fluid paste 23 gauge/easy 1 minute 0.15-0.25 37 PEG 300 ™ 63 paste 22 gauge/easy 1 minute 0.15-0.25 50 PEG 300 ™ 50 paste 22 1 minute gauge/moderate 0.15-0.25 24 PEG 300 ™ 50 Diblock 26 paste 22 1-2 gauge/moderate minutes 0.15-0.25 37 PEG 300 ™ 50 Diblock 13 paste 22 gauge/easy- 1-2 moderate minutes 0.15-0.25 40 PEG 750 ™ 60 paste 22 gauge/ 1-2 moderate minutes 0.15-0.25 40 PEG 900 ™ 60 paste 22 3-5 gauge/difficult minutes 0.15-0.25 30 PEG 1450 ™ 70 Wax/paste 16 1 minute gauge/difficult/ needs 37° C. 0.15-0.25 40 MethoxyPEG 60 paste 22 gauge/ 1-2 750 moderate minutes 0.15-0.25 50 Propylene 50 Very viscous 16 gauge/difficult 1 hour Glycol paste 0.25-0.50 35 PEG 300 ™ 65 Medium 18 gauge/e 5 viscous 16 gauge/easy minutes paste 0.47-0.55* 50 PEG 300 ™ 50 Very viscous 16 1 hour paste gauge/difficult 0.47-0.55* 40 PEG 300 ™ 60 viscous 16 gauge 0.5-1 paste easy hour 0.47-0.55* 30 PEG 300 ™ 70 paste 16 gauge 3-5 min easy 0.47-0.55* 20 PEG 300 ™ 80 liquid paste 16 gauge/very 1 easy minute, but dissolves away More viscous injectables (needs pressure for injection - waxy prior to injection, delayed set time) 0.25-0.50 35 Propylene 65 Almost 16 1-2 hour glycol solid/paste gauge/extreme force 0.15-0.25 50 Pluronic 50 Very viscous 16 1 hour L101 ™ paste gauge/difficult PLLA 2K 40 PEG 300 ™ 60 Wax 16 5 gauge/extreme minutes PCLdiol 60 PEG 300 ™ 40 Wax 18 2 1250 gauge/difficult minutes sets to v hard implant Not injectable using normal gauge needles or a reasonable amount of force 0.55-0.75 20 PEG 300 ™ 80 No Not injectable n/a homogenous paste 0.55-0.75 30 PEG 300 ™ 70 No Not injectable n/a homogenous paste 0.55-0.75 40 PEG 300 ™ 60 No Not injectable n/a homogenous paste 0.55-0.75 50 PEG 300 ™ 50 No Not injectable n/a homogenous paste *the PLGA was not from Durect ™ and the IV values for these PLGAs were estimated based on the ratio of LA:GA of 75:25.

(38) Drug delivery compositions described herein may exist in a variety of “paste” forms. Examples of paste forms may include liquid paste, paste or wax-like paste, depending on to polymers used, the amount of the polymers used and the temperature.

(39) Drug delivery compositions described herein may release one or more drugs over a period of several hours or over several months, depending on the need. Compositions described herein may be used for localized delivery of one or more drugs to a subject. Examples of drugs that may be delivered using these compositions are not limited, and may include anti-cancer drugs, anti-inflammatory agents, anti-bacterial, anti-fibrotic, analgesic. Examples of anti-cancer drugs that may be used with the compositions of the present invention include docetaxel, paclitaxel, mitomycin, cisplatin, etoposide vinca drugs, doxorubicin drugs, rapamycin, camptothecins, gemcitabine, finasteride (or other cytotoxics); bicalutamide, enzalutamide, VPC-27, tamoxifen, sunitinib, erlotinib. Anti-cancer biological agents may also be used in the formulation such as antibody based therapies e.g. Herceptin, Avastin, Erbitux or radiolabelled antibodies or targeted radiotherapies such as PSMA-radioligands. Anti-inflammatory agents may include non-steroidal drugs like ibuprofen, steroids like prednisone. Local analgesia or local anesthetic medications may include, for example, one or more of the following: Procaine; Benzocaine; Chloroprocaine; Cocaine; Cyclomethycaine; Dimethocaine/Larocaine; Piperocaine; Propoxycaine; Procaine/Novocaine; Proparacaine; Tetracaine/Amethocaine, Articaine; Bupivacaine; Cinchocaine/Dibucaine; Etidocaine; Levobupivacaine; Lidocaine/Lignocaine/Xylocaine; Mepivacaine; Prilocaine; Ropivacaine; and Trimecaine. Antibiotic medications may include penicillin, cephalexin, gentamicin, ciprofloxacin, clindamycin, macrodantin, and others. The drugs may be hydrophobic or may be hydrophilic. Specific drugs may be selected from one of more of the following: Docetaxel; VPC-27; Bicalutamide; Cephalexin (A); Sunitinib; Tamsulosin; Desoximetasone; Gemcitabine; Rapamycin; and Ibuprofen.

(40) Hydrophobic drugs may be able to bind with strong affinity to the hydrophobic water-insoluble polymer (ex. PLGA) allowing slow dissociation and controlled release from the implant. Such drugs tend to dissolve (at least partially) in the paste mixture. Hydrophilic drugs may be blended into the paste but because the matrix is partially hydrated in aqueous environments, these drugs may dissolve out of the implant quickly. In some situations this may be desirable, such as when an antibacterial drug may be included in the paste to treat a local infection and it is preferred if all the drug is cleared form the paste in 7 days to suit an antibacterial drug treatment regime.

(41) Drug delivery compositions may be prepared and utilized to treat or prevent a variety of diseases or conditions. Examples of diseases or conditions that may be treated, may for example, include cancer, pain, inflammatory conditions, fibrotic conditions, benign tumors (including benign prostate hyperplasia), and infections.

(42) Local anesthetics usually fall into one of two classes: aminoamide and aminoester. Most local anesthetics have the suffix “-caine”. The local anesthetics in the aminoester group may be selected from one or more of the following: Procaine; Benzocaine; Chloroprocaine; Cocaine; Cyclomethycaine; Dimethocaine/Larocaine; Piperocaine; Propoxycaine; Procaine/Novocaine; Proparacaine and Tetracaine/Amethocaine. The local anesthetics in the aminoamide group may be selected from one or more of the following: Articaine; Bupivacaine; Cinchocaine/Dibucaine; Etidocaine; Levobupivacaine; Lidocaine/Lignocaine/Xylocaine; Mepivacaine; Prilocaine; Ropivacaine; and Trimecaine. Local anesthetics may also be combined (for example, Lidocaine/prilocaine or Lidocaine/tetracaine).

(43) Furthermore, local anesthetics used for injection may be mixed with vasoconstrictors to increase residence time, and the maximum doses of local anesthetics may be higher when used in combination with a vasoconstrictor (for example, prilocaine hydrochloride and epinephrine; lidocaine, bupivacaine, and epinephrine; lidocaine and epinephrine; or articaine and epinephrine).

(44) Anti-cancer drugs as may be used in the composition described herein, may be categorized as alkylating agents (bi and mono-functional), anthracyclines, cytoskeletal disruptors, epothilone, topoisomerase inhibitors (I and II), kinase inhibitors, nucleotide analogs and precursor analogs, peptide antibiotics, platinum-based agents, vinka alkaloids, and retinoids. Alkylating agents, may be bifunctional alkylators (for example, Cyclophosphamide, Mechlorethamine, Chlorambucil and Melphalan) or monofunctional alkylators (for example, Dacarbazine (DTIC), Nitrosoureas and Temozolomide). Examples of anthracyclines are Daunorubicin, Doxorubicin, Epirubicin, Idarubicin, Mitoxantrone, and Valrubicin. Cytoskeletal disruptors or taxanes are Paclitaxel, Docetaxel, Abraxane and Taxotere. Epothilones may be epothilone or related analogs. Histone deacetylase inhibitors may be Vorinostat or Romidepsin. Inhibitors of topoisomerase I may include Irinotecan and Topotecan. Inhibitors of topoisomerase II may include Etoposide, Teniposide or Tafluposide. Kinase inhibitors may be selected from Bortezomib, Erlotinib, Gefitinib, Imatinib, Vemurafenib or Vismodegib. Nucleotide analogs and precursor analogs may be selected from Azacitidine, Azathioprine, Capecitabine, Cytarabine, Doxifluridine, Fluorouracil, Gemcitabine, Hydroxyurea, Mercaptopurine, Methotrexate or Tioguanine/Thioguanine. Peptide antibiotics like Bleomycin or Actinomycin. Platinum-based agents may be selected from Carboplatin, Cisplatin or Oxaliplatin. Retinoids may be Tretinoin, Alitretinoin or Bexarotene. The Vinca alkaloids and derivatives may be selected from Vinblastine, Vincristine, Vindesine and Vinorelbine.

(45) An anti-cancer drug that may be used with the compositions described herein, may be selected from one or more of: Actinomycin; All-trans retinoic acid; Azacitidine; Azathioprine; Bleomycin; Bortezomib; Carboplatin; Capecitabine; Cisplatin; Chlorambucil; Cyclophosphamide; Cytarabine; Daunorubicin; Docetaxel; Doxifluridine; Doxorubicin; Epirubicin; Epothilone; Etoposide; Fluorouracil; Gemcitabine; Hydroxyurea; Idarubicin; Imatinib; Irinotecan; Mechlorethamine; Mercaptopurine; Methotrexate; Mitoxantrone; Oxaliplatin; Paclitaxel; Pemetrexed; Teniposide; Tioguanine; Topotecan; Valrubicin; Vemurafenib; Vinblastine; Vincristine; Vindesine; and Vinorelbine. Alternatively, the anti-cancer drug may be a biological agent and may be selected from Herceptin (Trastuzumab), Ado-trastuzumab, Lapatinib, Neratinib, Pertuzumab, Avastin, Erbitux or radiolabelled antibodies or targeted radiotherapies such as PSMA-radioligands. The anti-cancer drug may be an Androgen Receptor, an Estrogen Receptor, epidermal growth factor receptor (EGFR) antagonists, or tyrosine kinase inhibitor (TKI). An anti-angiogenesis agent may be selected from avastin, an epidermal growth factor receptor (EGFR) antagonists or tyrosine kinase inhibitor (TKI). An Immune modulator such as Bacillus Calmette-Guerin (BCG).

(46) As used herein a “drug” refers to any therapeutic moiety, which includes small molecules and biological agents (for example, proteins, peptides, nucleic acids). Furthermore, a biological agent is meant to include antibodies and antigens. As used herein, the term drug may in certain embodiments include any therapeutic moiety, or a subset of therapeutic moieties. For example, but not limited to one or more of the potentially overlapping subsets and one or more drugs, as follows: hydrophobic drugs, hydrophilic drugs; a cancer therapeutic drug; a local anesthetic drug; an anti-biotic drug; an anti-viral drug; an anti-inflammatory drug; a pain drug; an anti-fibrotic drug; or any drug that might benefit from a localized and/or sustained release.

(47) As used herein, “an antibody” is a polypeptide belonging to the immunoglobulin superfamily. In particular, “an antibody” includes an immunoglobulin molecule or an immunologically active fragment of an immunoglobulin molecule (i.e., a molecule(s) that contains an antigen binding site), an immunoglobulin heavy chain (alpha (α), mu (μ), delta (δ) or epsilon (ε)) or a variable domain thereof (VH domain), an immunoglobulin light chain (kappa (κ) or lambda (λ)) or a variable domain thereof (VL domain), or a polynucleotide encoding an immunoglobulin molecule or an immunologically active fragment of the immunoglobulin molecule. Antibodies includes a single chain antibody (e.g., an immunoglobulin light chain or an immunoglobulin heavy chain), a single-domain antibody, an antibody variable fragment (Fv), a single-chain variable fragment (scFv), an scFv-zipper, an scFv-Fc, a disulfide-linked Fv (sdFv), a Fab fragment (e.g., CLVL or CHVH), a F(ab′) fragment, monoclonal antibodies, polyclonal antibodies. As used herein “antigen” refers to any epitope-binding fragment and a polynucleotide (DNA or RNA) encoding any of the above.

(48) As used herein, a “paste” is any composition described herein that has the characteristics of a solid and of a liquid depending on applied load and the temperature. Specifically, the viscosity of a paste may be anywhere in the range of about 0.1 to about 200 pascal seconds (Pa.Math.s) at room temperature and may be measured by any number of methods known to those of skill in the art. Numerous types of viscometers and rheometers are known in the art. For example, a cone and plate rheometer (i.e. Anton Paar™, MCR 502).

(49) As used herein a “swelling agent” is meant to encompass any biocompatible agent that will increase the volume of a paste as described herein, once the paste with swelling agent incorporated is placed in an aqueous environment. A swelling agent may be selected from: salts of hyaluronic acid (e.g., sodium hyaluronate); cellulose derivatives (e.g., carboxymethylcellulose); or polyacrylic acid derivatives (e.g., Carbomers). A swelling agent may advantageously be approved for use in injectable compositions. Also having a swelling agent that does not interfere with the injectability of the paste (for example, is not too grainy, does not precipitate and is easy to disperse again) would be of benefit. It may also be advantageous, if the swelling agent is able to provide a suitable amount of swelling without reducing the overall % of the polymers of the paste as described herein (i.e. be a small percentage of the overall paste). Furthermore, a swelling agent that exhibits high rate of swelling and quick swelling characteristics (for example, swell within minutes of injection) would be beneficial.

(50) Methods

(51) Paste Preparation

(52) The paste was prepared by weighing the polymers into a glass vial and stirring at 60° C. The polymers formed a homogenous melt. If drug is to be added, it is added following the polymer paste preparation. The values for the paste polymers (i.e. hydrophobic water-insoluble polymer; low molecular weight biocompatible glycol; and, if used, the di-block copolymer and/or swelling) is prepared as a total % out of 100% before mixing with drug. When the drug is added the % associated therewith is a percent of the total composition with drug and the “pre-drug paste” component %s are based on their proportions prior to adding the drug. For example, 4% means 4 g of drug in 100 g paste. Drug(s) were incorporated using levigation or a mortar and pestle.

(53) The injectability of a paste will depend on many parameters (i.e. needle size, needle lengths, volume, tissue backpressure, strength of the person administering the paste). Normally it is preferred that a paste be easily drawn up into a syringe using a 14 gauge needle and easily injected into a tissue zone using an 18 gauge or even smaller needle with a small amount of extra pressure. However, for particular uses and depending on the gauge of the needle, having a more viscous paste (i.e. more difficult to inject), may be desirable.

(54) Viscosity Measurement

(55) Viscosity measurements were taken using a cone plate rheometer (Anton Paar™, MCR 502) and recorded as a function of shear rate at constant temperature.

(56) Water Absorption by Pastes Containing a Swelling Agent

(57) Pastes containing a swelling agent were prepared by incorporating increasing amounts of a base paste (PEG:PLGA) into the swelling agent (sodium hyaluronate) using mortar and pestle. Around 20 mg of paste samples (n=3) for each paste formulation were weighed on filter membranes (0.45 μm) and repeatedly weighed after soaking in water at 37° C. For each time point, excess water was carefully removed using a vacuum pump.

(58) In Vitro Drug Release Assays

(59) The drug-loaded paste can be aliquoted for in vitro release studies. Paste (50-100 mg) is deposited at the bottom of a test tube and release medium is added (5-10 mL, sink conditions). Release medium is phosphate buffered saline (PBS, 10 mM, pH 7.4)) or PBS containing 1% Albumin. The test tubes are kept in a 37° C. incubator until the end of the study. Release samples are taken at appropriate time points by replacing the complete release medium (supernatant) and analyzing it for total drug using Reversed phase high-performance liquid chromatography with ultraviolet (UV) detection (RP-HPLC-UV).

(60) TABLE-US-00002 TABLE 1 Chromatographic parameters used in RP-HPLC Parameter Specification HPLC Waters (1525 Binary HPLC Pump, 2489 UV/Visible Detector, 717 plus Autosampler) Detector UV/Visible Flow rate 1 mL/min Column C-18, Nova-Pak, 4 μm, 3.9 × 150 mm Column Temperature Ambient, no temperature control Injection Volume 20 μL Elution isocratic

(61) TABLE-US-00003 TABLE 2 Mobile phase, retention times and UV detection wavelength for studied drugs. Retention UV detection Mobile phase composition time wavelength Drug (v/v) (min) (nm) Bicalutamide/ 30/30/40 3 min 272 enzalutamide Acetonitrile/methanol/water (adjusted to pH 3.4 with glacial acetic acid) Docetaxel 30/30/40 6 min 228 Acetonitrile/methanol/water (adjusted to pH 3.4 with glacial acetic acid) VPC-27 300/180/35 4.3 min 244 Acetonitrile/methanol/water + 200 μL glacial acetic acid Rapamycin 200/175/125 2.9 min 278 Acetonitrile/methanol/water Cephalexin 20/80 5 min 254 Acetonitrile/water (adjusted to pH 3.4 with glacial acetic acid) Lidocaine 50/50 3.2 min 220 Acetonitrile/ammoniumacetate 20 mM (pH 6.4) Desoximetasone 50/50 2 min 244 Acetonitrile/ammoniumacetate 20 mM (pH 6.4) Sunitinib 55/45 4 min 431 Acetonitrile/ammoniumacetate 20 mM (pH 6.4) Tamsulosin 50/50 2.3 min 220 Acetonitrile/ammoniumacetate 20 mM (pH 6.4) Ibuprofen 50/50 1.9 min 220 Acetonitrile/ammoniumacetate 20 mM (pH 6.4) Gemcitabine 3/97 2.3 min 272 methanol/water (adjusted to pH 3.4 with glacial acetic acid)

(62) Intratumoral Paste Injection

(63) Athymic male nu/nu mice (uncastrated) have been injected with 4×10.sup.6 LNCap cells suspended in Matrigel™ subcutaneously in the left flank. Treatment allocation began once a single site tumor reached 150-200 mm.sup.3 via caliper measurement. Drug-loaded paste (30 μL) was injected into the tumor using a 21 gauge needle. Serum prostate specific antigen (PSA) levels were measured over time and tumor size was monitored.

(64) In another experiment, groups of five to six animals received 30-40 μL paste intratumorally once the tumor had reached a size of 100 mm.sup.3. Tumor growth and serum PSA levels were monitored for the following 12 weeks. Local delivery of paste subcutaneously in rats.

(65) Five groups of rats (male, Sprague Dawley™) with six animals in each group received one injection of paste formulation (0.1 mL) subcutaneously in their flank. The paste formulation was based on a 50:50 mixture of PEG 300™ and PLGA. Lidocaine was incorporated into the paste at 80, 100, 120, 140, and 160 mg per g of paste. The corresponding doses for each group were 23, 29, 36, 40 and 45 mg of Lidocaine per kg.

(66) Blood was collected from the saphenous vein over four weeks at 0, 0.25, 1, 4, 24, 48, 168 336, 504 and 672 hours after injection. Lidocaine concentrations in rat serum were determined using ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). A non-compartmental analysis was then applied to each data set using Phoenix 64™ (Build 6.3.0.395) WinNonlin 63™ to determine relevant pharmacokinetic parameters.

(67) Kidney Pelvis Injection of Paste

(68) After placement of a ureteral catheter, three pigs received an injection of 1-2 mL of polymeric paste into the kidney pelvis. After removal of the ureteral catheter, a urinary catheter was placed and urine collected for 3 h intervals over 24 h. Blood was collected from an ear vein over 24 h and gemcitabine concentrations were determined using ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).

EXAMPLES

Example 1

Viscosity of Polymeric Pastes Manufactured Using Different Ratios of PLGA, PEG and Diblock Copolymer

(69) The polymeric paste is a biocompatible formulation comprised of two or three constituents: poly-(lactic-co-glycolic) acid (PLGA), a diblock copolymer of DL-lactide (DLLA) (optional) and methoxy polyethylene glycol (mePEG) termed poly(DL-lactide)-methoxy polyethylene glycol (PDLLA-mePEG), and polyethylene glycol with a molecular weight of 300 Da (PEG 300™). Drug or drug mixtures can be incorporated into the paste through levigation with a spatula and the paste can be injected through a 20 G needle. The PLGA is comprised of equal amounts of LA and GA (50:50 Poly[DL-lactide-co-glycolide]) and may have a degradation time of 1-2 months. The polymeric drug delivery paste may be an injectable viscous solution at 37° C. After injection into an aqueous tissue environment, the paste may transform into a waxy solid, which may serve as a sustained release platform for incorporated drug(s).

(70) Polymeric pastes were manufactured using different weight ratios of PLGA (Durect™, Alabama) (IV=0.15-0.25 dL/g, 50:50 ratio of LA to GA), PEG with a molecular weight of 300 Da (Polysciences™, USA) and diblock copolymer (synthesized in house, MW=3333 Da, comprising 40% PLLA and 60% methoxypolyethylene glycol (w:w)). The diblock copolymer may be used to adjust the degradation profile of the polymeric paste and the release profile of the drug(s). The diblock copolymer can help to encapsulate hydrophobic drugs due to its amphiphilic characteristics. In aqueous solution, the diblock can spontaneously arrange itself in micelles that can host hydrophobic drug in the poly (DL lactic acid) (PDLLA) core surrounded by the hydrophilic PEG or mePEG chains.sup.53-55.

(71) The components were weighed into a glass vial and stirred overnight at 37° C. to form a homogenous mixture. Viscosity measurements were taken using a cone plate rheometer (Anton Paar™, MCR 502) and recorded as a shear rate at constant temperature. Pastes comprised of PEG and PLGA had very low viscosities at low PLGA content such that the viscosity of pastes at 80% PEG were less than 1 Pa.Math.s. FIG. 1 shows that as the concentration of PLGA increased, paste viscosity rose very quickly to over 100 Pa s for the 40% PEG and 60% PLGA composition. FIG. 1 also shows that the addition of diblock copolymer in place of PLGA reduced the viscosity considerably so that at 13% diblock with 50% PEG and 37% PLGA the viscosity was less than 10 Pas, and was reduced even further using 26% diblock.

Example 2

The Release of PEG 300™ from Polymeric Paste

(72) Seven compositions of polymeric pastes were manufactured from PLGA, PEG and diblock copolymer as described in EXAMPLE 1. For each of the seven different polymeric pastes, 8×100 mg each were weighed into the corner of 8×20 ml pre-weighed scintillation vials by holding each vial at a slight angle. 10 ml of water was added to each vial with the vial still held at an angle so the paste remained in the vial corner whilst exposed to water. After 10 minutes the outer surface of each paste whitened slightly indicating setting of the paste and the vials were then reoriented to the vertical position. This procedure prevented a premature disruption of the setting paste upon exposure to water turbulences. The vials were capped and placed in a 37° C. oven. At various time points the vials were removed, water was aspirated and the contents dried for one hour in a 37° C. incubator followed by one day of vacuum drying at room temperature. The vials were then re-weighed to determine the weight loss of water-soluble polymer (PEG or diblock) that dissolved into the water from the polymer paste. FIG. 2 shows that most of the PEG or diblock was released by 2 days.

(73) The values of % polymer released approximately matched the initial weight of PEG and diblock present by % in each formulation.

Example 3

Release of Docetaxel, VPC-27 and Bicalutamide from Polymeric Pastes

(74) Polymeric pastes were manufactured from 50:50, 55:45, 60:40 weight ratio compositions of PLGA (50:50 IV=0.15-0.25 dL/g) and PEG 300™, 50:37:13 ratios of PEG:PLGA:Diblock, or 50:24:26 ratios of PEG:PLGA:Diblock using the methods described in Example 1. The presence of the diblock copolymer allows more detailed control of drug release. This diblock copolymer has been previously described to increase the water solubility of hydrophobic drugs by forming diblock micelles with hydrophobic cores that allows the drugs to partition into the core and increase the apparent solubility. In the paste application as water enters the paste matrix the water soluble diblock begins to dissolve out and any drug dispersed at the molecular level may become “micellized” in the diblock milieu to increase drug release. The drugs VPC-27, bicalutamide or docetaxel were added at various weight ratios directly into the paste with mixing by standard spatula levigation techniques. The drug release was studied according to the descriptions found in the general methods section (In vitro drug release assays, TABLE 1 and TABLE 2).

(75) FIG. 3 shows the release of VPC-27, docetaxel and bicalutamide from a 50:50 (PEG:PLGA) paste. Docetaxel (at 4% w/w) and bicalutamide (at 4% w/w) released with similar profiles showing a fast burst of release over 5 days (10% drug released) followed by a slower more sustained release over the next 40 days where a further 20% of encapsulated drug was released (FIG. 3A). VPC-27 (at 10% w/w) released slowly from the paste reaching about 15% of total encapsulated drug released by day 45 (FIG. 3A). When the concentration of VPC-27 was reduced to 4%, the release profiles of docetaxel and bicalutamide were similar to each other but a little slower overall than from the paste containing 10% VPC-27. However, the release rate of VPC-27 was almost the same as the other two drugs using a 4% w/w drug loading (FIG. 36).

(76) FIG. 4 shows the release of docetaxel, Bicalutamide, and VPC-27 from PEG:PLGA:Diblock pastes 50:37:13 w/w % or 50:24:26 w/w %. FIG. 4A shows that the release rate of docetaxel from 13% and 26% diblock loaded pastes is increased in comparison to the 50:50 paste mixtures shown in FIG. 3 (no diblock). FIG. 4A further shows that by day 10, between 40 and 60% of encapsulated docetaxel was released from the 13% and 26% diblock pastes as compared to less than 17% being released from the 50:50 pastes shown in FIG. 3. The addition of diblock copolymer to the PEG:PLGA paste had a similar effect on the release rates of bicalutamide (FIG. 4B) and VPC-27 (FIG. 4C). By day 10, the release was approximately 60% for both drugs at a diblock loading of 13%, and approximately 100% for pastes containing 26% diblock compared to less than 17% release for either drug from 50:50 pastes with no diblock.

(77) FIG. 5 shows the release of docetaxel (0.25%), bicalutamide (4%), and VPC-27 (4%) w/w) from a PEG:PLGA:Diblock paste (50:37:13 w/w %). Drug release was characterized by a small burst release within the first day and a slower release of drug over the next 35 days. After one day, 24% docetaxel, 8% bicalutamide and 12% VPC-27 were released, followed by a slow release reaching 32% docetaxel, 16% bicalutamide and 19% VPC-27, on day 35 (FIG. 5).

(78) FIG. 6 shows the effect of varying PLGA:Diblock ratios on the release rates of docetaxel (0.5%), bicalutamide (4%) and VPC-27 (4%) from PEG:PLGA:Diblock pastes (50:Y:X w/w %). Diblock (X) varied from 7, 13, 19 to 25% and PLGA (Y) varied accordingly from 43, 37, 31 to 25% w/w %.

(79) FIG. 6 shows that by increasing diblock paste content (7%, 13%, 19%, 25%) and decreasing PLGA content, drug release rates increased. For example, at the low diblock concentration, docetaxel was released in a burst of 20% on day 1 and reached 34% on day 21 (FIG. 6A); while at the high diblock concentration, docetaxel was released in a burst of 67% on day 1 and reached 78% on day 21 (FIG. 6D). The effect was similarly pronounced for bicalutamide and more pronounced for VPC-27 (FIG. 6).

(80) FIG. 7 shows how high amounts of PEG affects the release rates of docetaxel (4%), bicalutamide (4%) and VPC-27 (4%) from PEG:PLGA pastes (63:37 or 76:24). At too high a ratio of PEG to PLGA the paste may be very fluid and tends to disintegrate in vivo because the PLGA is over dispersed and unable to form a cohesive solid. Docetaxel released quickly from the high PEG content pastes reaching between 40% and 50% released drug at day 11 (FIG. 7A). Neither bicalutamide nor VPC-27 released quickly from either of the high PEG pastes, but release was steady and continuous even after 28 days (FIGS. 7B and 7C). At 11 days, drug release from either high PEG paste formulation was below 22% for both bicalutamide and VPC-27.

(81) FIG. 8 shows more release profiles of docetaxel (1%), bicalutamide (4%), and VPC-27 (4%) from PEG:PLGA pastes without diblock. Three pastes were prepared with 50:50, 55:45, 60:40 PEG:PLGA (w/w %). The pastes stayed cohesive and the release of the drugs increased with increasing PEG content. The release of the three drugs in the 50:50 paste was around 2-5%, for the 55:45 paste around 10% and for the 60:40 paste between 10 and 20% on day 18.

Example 4

Release of Rapamycin from Polymeric Pastes

(82) Polymeric pastes comprised of 50% PEG300, 37% PLGA (IV 0.15, 50:50 ratio) and 13% diblock copolymer containing a drug mixture of docetaxel, rapamycin and VPC-27 (at 1%, 1% and 4% w/w, respectively) were manufactured as described in the general methods section. Drug release experiments were performed as described previously (In vitro drug release assays, TABLE 1 and TABLE 2).

(83) FIG. 9 shows the release rates for docetaxel, rapamycin, and VPC-27, from PEG:PLGA:Diblock pastes (50:37:13). Docetaxel released in a sustained manner reaching almost 45% drug release at day 8. VPC-27 released well, reaching almost 20% release at day 8. Rapamycin released very slowly with only 4% of the encapsulated drug being released at day 8.

Example 5

Release of Cephalexin from Polymeric Paste

(84) Polymeric pastes comprised of 50% PEG300, 37% PLGA (IV 0.15, 50:50 ratio) and 13% dibock copolymer containing cephalexin at between 2 and 19% loading were prepared as described in Examples 1 and 3. For cephalexin, albumin was not included in the PBS as this drug is water soluble. The drug release was studied according to the descriptions found in the general methods section (In vitro drug release assays, TABLE 1 and TABLE 2). FIG. 10 shows that Cephalexin released quickly from the polymeric pastes where almost all drug was released from drug loaded pastes at 1 day.

Example 6

Effect of Paste Geometry and Drug Loading on the Release of Lidocaine from Polymeric Paste

(85) PEG:PLGA:Diblock paste (50:37:13) containing lidocaine (non-HCl form) at 2-10% w/w loading were mixed as previously described. To achieve different paste geometries, the 8% w/w paste was placed in a syringe and 100 mg samples were extruded through an 18 gauge needle onto the base of a cold (approximately 2° C.) 20 ml glass scintillation vial as either a cylinder, a crescent shape in the lower corner of a tilted vial or as a hemisphere “blob” in the middle of the base of the vial. The cold temperature assisted in keeping the shape of the very viscous paste at this temperature. 10 ml of cold PBS were very gently added and the vial was left for 10 minutes to allow the outer surface of the paste to whiten a little. The vials were then placed in a 37° C. incubator. At dedicated time points the 10 ml of PBS were removed and replaced with another 10 ml of room temperature PBS. The drug release was studied according to the descriptions found in the general methods section (In vitro drug release assays, TABLE 1 and TABLE 2).

(86) Lidocaine released from all geometric forms with a burst phase between approximately 50% and 70% at day 2 (FIG. 11A). After that the release rate slowed, especially for the hemisphere shape, such that by day 7 this shape had released approximately 65% of the drug as compared to 88% and 92% released from the crescent and cylinder shapes, respectively. All paste shapes released small amounts of lidocaine between 7 to 28 days. The release rates for lidocaine using different % w/w loadings were similar and are shown in FIG. 11B.

Example 7

Release of Docetaxel, VPC-27 and Enzalutamide from Polymeric Pastes

(87) Polymeric pastes were manufactured from 63:37 compositions of PEG 300™ and PLGA (50:50 IV=0.15) or from 50:37:13 ratios of PEG:PLGA:Diblock using the method described earlier. The drugs VPC-27, enzalutamide or docetaxel were added at various weight ratios directly into the paste with mixing by standard spatula levigation techniques. The drug release was studied according to the descriptions found in the general methods section (In vitro drug release assays, TABLE 1 and TABLE 2). All drugs released more quickly from the diblock containing paste than the high PEG content paste as shown in FIG. 12. From the high PEG content PEG:PLGA paste (63:37), VPC-27 released slowly without any apparent burst phase of release (FIG. 12C) but a burst phase occurred from the PEG:PLGA:diblock containing paste (50:37:13), resulting in nearly 35% of drug released by day 50 (FIG. 12C). Docetaxel released well from both pastes (approximately 50% released by day 50) (FIG. 12A) and enzalutamide released approximately 40% of the total encapsulated drug by day 50 (FIG. 12B).

Example 8

Solubilization of Drugs by Diblock Copolymer

(88) Diblock copolymer (molecular weight 3333, PLLA 40%, MePEG 2000 60%) was weighed out into 2 ml glass vials in various amounts at concentrations of 0 to 45 mg/ml. The drugs docetaxel, bicalutamide, and VPC-27 were added in a drug polymer ratio of 1:9 (one part drug, 9 parts diblock copolymer) from stock solutions in acetonitrile and topped up to approximately 1 ml. All contents were in solution and the vials were dried down under nitrogen with mild heat followed by vacuum overnight. The vials were then warmed to 37° C. and 1 ml of PBS at 37° C. was added. The vials were vortexed to dissolve their contents and the contents were then centrifuged at 15000 rpm in a microfuge and filtered through a 0.2 μm filter to give a clear solution. The concentration of each drug in each solution was then measured using RP-HPLC described in the general methods section (In vitro drug release assays, TABLE 1 and TABLE 2). Drugs were solubilized effectively by the diblock copolymer as shown in FIG. 13. For VPC-27 and docetaxel, drug concentrations in the 3.5 to 5 mg/ml range were achieved. Above a diblock concentration of 20 mg/ml, bicalutamide did not stay in solution as shown in FIG. 13.

Example 9

Release of Lidocaine (10%) and Desoximetasone (1%) from PEG:PLGA:Diblock Paste (50:37:13

(89) The paste was manufactured as in Example 7 using lidocaine at 10% and desoximetasone at 1%. Drug release was measured using RP-HPLC as described earlier (In vitro drug release assays, TABLE 1 and TABLE 2).

(90) FIG. 14 shows that lidocaine released in the same manner as previously observed with a 50% burst within the first day and an extended release reaching 80% by day 35. Similarly, desoximetasone released with a burst of 50% on day one and reached 100% release on day 35. The injectable lidocaine pastes keep the drug near the local injection site and delays systemic uptake of lidocaine. The suggested drug load is 10% and the maximum injectable volume should be 3 g of paste into the spermatic cord to stay below the maximum single dose of 300 mg. Lidocaine is released in a sustained fashion while the paste degrades.

Example 10

Release of Lidocaine from Various PEG:PLGA Pastes without Diblock

(91) Pastes were manufactured as described in Example 1. FIG. 15 shows the lidocaine release from PEG:PLGA pastes without diblock. Three pastes were prepared with 50:50, 55:45, 60:40 PEG:PLGA (w/w %) and 8% lidocaine (w/w). The pastes stayed cohesive and drug release was faster in pastes with higher PEG content. Lower PEG content decreased the amount of burst release slightly and by day 18, 100% of lidocaine was released from all pastes.

Example 11

Release of Sunitinib from PEG:PLGA:Diblock Polymeric Paste (50:37:13

(92) The paste was manufactured as in Example 7 and the drug Sunitinib was added at 1% w/w. Drug release was measured using RP-HPLC as described earlier (In vitro drug release assays, TABLE 1 and TABLE 2). The release of Sunitinib is shown in FIG. 16 and is characterized by a burst phase on day 1 with a release of 35% and an extended release phase that reaches approximately 55% at day 44.

Example 12

Release of Tamsulosin from PEG:PLGA:Diblock Polymeric Paste (50:37:13

(93) Tamusolin was loaded at 2% w/w to PEG:PLGA:Diblock polymeric paste (50:37:13 PEG:PLGA:Diblock) as described in Example 6. Drug release was measured using RP-HPLC as described earlier (In vitro drug release assays, TABLE 1 and TABLE 2). The release of Tamsulosin is shown in FIG. 17 and is characterized by a burst phase of 47% on day 1. The release continues and reaches 70% by day 6 and 80% on day 21.

Example 13

Release of Lidocaine (8%), Cephalexin (2%) and Ibuprofen (5%) from PEG:PLGA:Diblock Polymeric Paste (50:37:13

(94) The three drugs were loaded into the paste as previously described in Example 3. HPLC analysis for lidocaine, cephalexin and ibuprofen was performed using the general chromatographic set up mentioned earlier (In vitro drug release assays, TABLE 1 and TABLE 2). The release of the three drugs is shown in FIG. 18. Cephalexin was released very quickly and reached its maximum at 80% on day 3. Lidocaine and Ibuprofen release was similar and characterized by a burst release of 54% drug by day one and a slower drug release over the next 10 to 15 days, reaching approximately 70% by day 35.

Example 14

Effect of Drug Loaded Polymeric Paste on the Growth of Human Prostate Cancer Tumors in Mice

(95) PEG:PLGA:Diblock polymeric paste (50:37:13) containing docetaxel (1%), bicalutamide (1%), and VPC-27 (4%) was manufactured as previously described and injected intra-tumorally (see Intratumoral paste injection, FIG. 19).

(96) In a different experiment, groups of mice were treated with a formulation containing either docetaxel alone, bicalutamide and docetaxel, docetaxel and VPC-27 or all three drugs. Treatment groups that received both docetaxel and bicalutamide or docetaxel alone showed slower tumor growth and a delayed increase in serum PSA levels than groups that received pastes that contained also VPC-27.

Example 16

Local Release of Lidocaine and Absence from Serum In Vivo

(97) Five groups of rats (male, Sprague Dawley) with six animals in each group received one injection of paste formulation (0.1 mL) subcutaneously in their flank. The paste formulation was based on a 50:50 mixture of PEG 300™ and PLGA. Lidocaine was incorporated into the paste at 80, 100, 120, 140, and 160 mg per g of paste. The corresponding doses for each group were 23, 29, 36, 40 and 45 mg of lidocaine per kg. Lidocaine dissolved at all concentrations to form a clear paste except at the 160 mg/g level, where small crystals were visible that dissolved when warming the paste to 37° C. All formulations were warmed to 37° C. before administration and the injection was smooth.

(98) The concentrations of systemic lidocaine detected in serum were very low (see FIGS. 22A and 22B). The maximum concentrations detected in serum in each group were all below the upper limit of its therapeutic range of 5 μg/mL or 5000 ng/mL in all studied strengths. The maximum serum concentrations for the 80, 100, 120, 140, 160 mg/g paste formulations were 557.26±81.15, 578.90±162.59, 638.03±190.61, 855.98±196.18, 1148.88±838.97 ng/mL respectively. Since the highest dosing level (160 mg/g paste or 45 mg/kg) is 10 times above the locally administered lidocaine dose in humans (4.5 mg/kg) using a conventional lidocaine solution.

Example 17

Use of Swelling Agents in Paste

(99) Pastes were manufactured using 68% PEG 300™, 30% PLGA and 2% of a swelling agent. The agents included carboxymethylcellulose, carbomer or sodium hyaluronate. These pastes were effectively injected through a 5 F ureteral catheter of 70 centimeters length. In water there was a clearly observed swelling behavior.

(100) The drug gemcitabine was incorporated at 5% (m/m) in the sodium hyaluronate containing paste. This paste was injected through the 5 F catheter into the kidney pelvis of a pig. Gemcitabine levels in urine were initially high and levelled off after 5-7 hours (see FIG. 25A). Serum gemcitabine levels were very low, but detectable in all pigs (see FIG. 25B). The paste did not result in any blockage of the ureter and paste fragments were found on the urinary catheter after removal.

Example 18

Use of Swelling Agent, Sodium Hyaluronate, for Gemcitabine Release

(101) Pastes containing 2% of sodium hyaluronate (SH) and increasing amounts of diblock copolymer were prepared to observe swelling and degradation of these pastes over one hour. The inclusion of SH was associated with a rapid swelling of the paste in water.

(102) As shown in FIG. 23, pastes with 2% SH were swelling rapidly after contact with water. Pastes with 0 and 10% of diblock absorbed less water than pastes with higher diblock copolymer content. Furthermore, these pastes did also not disintegrate over the monitored time. Pastes that contained 20, 30 and 40% diblock copolymer immediately absorbed water to roughly double their initial weight. The 20% paste hold its weight at least over one hour, whereas the 30% and 40% DB pastes lost 30 and 100% of their initial weight over one hour. Overall, a higher amount of DB allows for more rapid water absorption and swelling and quick paste disintegration.

Example 19

Release of Gemcitabine from Various Polymeric Pastes

(103) As shown in FIG. 24, pastes having no diblock copolymer showed delayed release of gemcitabine, except where there was a high PEG 300 level (i.e. 76%) and low PLGA (i.e. 22%). All other compositions showed a burst release at between 0 and 4.5 hours and sustained release thereafter.

(104) Although various embodiments of the invention are disclosed herein, many adaptations and modifications may be made within the scope of the invention in accordance with the common general knowledge of those skilled in this art. Such modifications include the substitution of known equivalents for any aspect of the invention in order to achieve the same result in substantially the same way. Numeric ranges are inclusive of the numbers defining the range. The word “comprising” is used herein as an open-ended term, substantially equivalent to the phrase “including, but not limited to”, and the word “comprises” has a corresponding meaning. As used herein, the singular forms “a”, “an” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a thing” includes more than one such thing. Citation of references herein is not an admission that such references are prior art to an embodiment of the present invention. The invention includes all embodiments and variations substantially as hereinbefore described and with reference to the examples and drawings.

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