Calibration device for an optical detector and setting device for setting calibration points for the calibration device

Abstract

The present invention provides a calibration device for an optical detector comprising a light emitting diode 1, and an electronic circuit 3 for driving the light emitting diode 1, wherein the electronic circuit 3 is a constant current generator configured to supply a constant driving current to the light emitting diode 1 in the range of 0.5 to 25 μA. Furthermore, the present invention provides a setting device for setting at least one calibration point for a calibration device as described above. The setting device comprises a light detector 51 configured to output a signal representing the light emission intensity of a calibration device under examination, a controller 53 for controlling the constant driving current of the calibration device under examination, and a calibration point setting means 55 for setting at least one calibration point for the calibration device under examination.

Claims

1. A calibration device for an optical detector comprising: a light emitting diode (1); and an electronic circuit (3) for driving the light emitting diode (1); wherein the electronic circuit (3) is a constant current generator (3) configured to supply a constant driving current to the light emitting diode (1) in the range of 0.5 to 25 μA wherein the current generator (3) comprises a first operational amplifier (31) and a shunt resistor (33); wherein the shunt resistor (33) is connected in series with the light emitting diode (1); and wherein the first operational amplifier (31) is provided for supplying the constant driving current to the light emitting diode (1) and is connected to compare a set input voltage (V2) with a voltage obtained based on a voltage drop across the shunt resistor (31).

2. The calibration device according to claim 1, wherein the driving current is in the range of 1 to 10 μA, preferably 1.5 to 4.5 μA and most preferably 2 to 4 μA.

3. The calibration device according to claim 1, further comprising: a diffusor (5) surrounding the light emitting diode (1).

4. The calibration device according to claim 1, further comprising: a switching device (9) arranged so as to energise the light emitting diode (1) upon an axial compression of a pen like casing (7) accommodating the light emitting diode (1).

5. The calibration device according to claim 1, further comprising: a setting unit (11) for setting the driving current.

6. The calibration device according to claim 5, wherein the setting unit (11) comprises an interface (13) for connecting an external current controller (53) for controlling the setting of the constant driving current based on a feed-back control using a light detector (51), for measuring the light output of the light emitting diode (1).

7. The calibration device according to claim 1, wherein the current generator (3) is configured to be switchable between a plurality of predetermined constant driving currents.

8. The calibration device according to claim 1, wherein the current generator (3) further comprises a second operational amplifier (35) connected as a low pass filter at a first node between the output of the first operational amplifier (31) and the shunt resistor (33).

9. The calibration device according to claim 8, wherein the current generator (3) further comprises a third operational amplifier (37) connected as voltage follower at a node between the shunt resistor (33) and the light emitting diode (1).

10. The calibration device according to claim 9, wherein the outputs of the second and third operational amplifiers (35, 37) are connected via respective resistors (R5, R7, R9) with the inverting input of the first operational amplifier (31).

11. A setting device for setting at least one calibration point for a calibration device according to claim 1, comprising: a light detector (51) configured to output a signal representing the light emission intensity of a calibration device under examination; a controller (53) for controlling the constant driving current of the calibration device under examination; and a calibration point setting means (55) for setting at least one calibration point for the calibration device under examination.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 a schematic view of a calibration device according to the invention;

(2) FIG. 2 a circuit diagram of the constant current generator of the calibration device of the invention;

(3) FIG. 3 a schematic view of a calibration device according to a further embodiment of the invention; and

(4) FIG. 4 the principal configuration of a setting device for the calibration device.

(5) In the following preferred embodiments of the invention will be described with reference to the accompanying drawings in detail.

(6) As shown in FIG. 1, a calibration device according to his invention is provided with a casing 7 having the shape of a pen.

(7) The pen like casing 7 accommodates a light-emitting diode (LED) 1 and an electronic circuit 3 for driving the light emitting diode 1. Additionally, the pen like casing 7 can accommodate an energy source, like a capacitor, a secondary cell or a battery, to power the electronic circuit for driving the light-emitting diode 1.

(8) Furthermore, as shown in FIG. 1, the light emitting diode 1 is provided within the pen like casing 7 surrounded by the diffusor 5, that is as semi-transparent area of the pen like casing 7, allowing the light emitted from the light emitting diode 1 to be irradiated to the outside of the pen like casing 7.

(9) The pen like casing 7 is formed resembling the usual form of a sample collecting devices, like a phial or a test-tube. The dimensions of the pen like casing 7 are such that it can be accommodated within an optical detector system for the inspection of bioluminescence.

(10) It is important for a reference light source to be representative of the light emitted by the real sample in practical use.

(11) For this propose the calibration device, respectively the reference light source is designed to have the same shape and to emit light only from a dedicated area as the real sample.

(12) The luminous intensity of a light-emitting diode 1 is directly proportional to the current applied through its junction.

(13) According to the invention a controlled current source generates a low current thought the light-emitting diode 1.

(14) The stabilized current source is advantageous over a voltage drop resistor because a forward voltage of the light-emitting diode 1 is temperature dependent and may lead to 13% variation within the range 278K to 308K.

(15) Compared to light source drives with luminescence feedback, where the light emission must be large enough to create a signal on a luminescence measuring device (photodiode, phototransistor . . . ), the current controlled light-emitting diode 1 generates directly a low luminance in open loop mode.

(16) Even so the temperature still influences the luminance intensity of the light-emitting diode 1 (about 16% in the range 136K to 375K2) mainly due to carrier lifetime change.

(17) However, this effect is acceptable, when considering that typically the detection devices operate only within the range 278K to 308K and are calibrated mainly at 295K. As the light-emitting diode 1 is driven with very low current the diode self-heating can be neglected.

(18) According to the invention the driving current of the light-emitting diode is set to be in the range of 0.5 to 25 μA.

(19) Preferably, the driving current is in the range of 1 to 10 μA, more preferably 1.5 to 4.5 μA and most preferably 2 to 4 μA.

(20) In this range the amount of light emitted by the light-emitting diode 1 is directly proportional to the driving current flowing through the light-emitting diode 1.

(21) The low current source proposed allows the light-emitting diode 1 to be supplied with a constant current regardless of any variation of its forward voltage drop.

(22) It should be kept in mind that the driving current has an exponential dependency from the driving voltage, so that a small variation of the voltage might cause a huge change of the driving current. This problem however can be avoided by the use of a constant current generator 3 as described herein.

(23) An example of the configuration of a corresponding constant current generator 3 is shown in FIG. 2.

(24) The electrical power source 39, like a secondary cell, a battery or a capacitor, is connected via a resistor R2 with the non-inverting input of a first operational amplifier 31. The additional resistor R3, which can be a controllable or variable resistor or a potentiometer, and which is connected between the resistor R2 and ground will act together with the resistor R2 as a voltage divider so as to set a voltage V2 at the non-inverting input of the first operational amplifier 31.

(25) The output of the first operational amplifier 31 is connected via a shunt resistor 33 (R1) with the input of the light-emitting diode 1, the output thereof is connected to ground.

(26) Furthermore, a first capacitor C1 and a resistor R6 are connected in series between a node N3 connected with the output of the first operational amplifier 31, and the inverting input of the first operational amplifier 31, for stabilizing the output of the first operational amplifier 31.

(27) Then constant current generator 3 of FIG. 2 is further provided with a second operational amplifier 35, which together with a second capacitor C2 and a further resistor R7, connected in parallel to each other, form a low-pass filter.

(28) The noninverting input of the second operational amplifier 35 is connected via a voltage divider, formed by resistors R4 and R8, with a node N1, provided between the output of the first operational amplifier 31 and the input of the shunt resistor 33.

(29) The output of this second power amplifier 35 is connected via a resistor R9 with the inverting input of the first operational amplifier 31.

(30) The inverting input of the second operational amplifier 35, on the other hand, is connected via a resistor R5 with the output of a third operational amplifier 37.

(31) The third operational amplifier 37 is configured as a voltage follower, that is the output thereof is fed back to the inverting input of the third operational amplifier 37.

(32) The noninverting input of the third operational amplifier 37 is connected with a further node N2, provided at the output side of the shunt resistor 33 and at the input side of the light-emitting diode 1.

(33) With this configuration the voltage V2 at the noninverting input of the first operational amplifier 31 is compared with a second voltage obtained from the voltage drop occurring at the shunt resistor 33, to which the driving current is applied.

(34) By an appropriate setting of the values of the resistors R1 to R9, the capacitors C1 and C2 and on the electrical power source 39 it is possible to obtain a highly stable driving current flowing through the light-emitting diode 1 and having a volume in the range between 0.5 and 25 μA.

(35) With the very low intensity supply to the LED by the constant current generator 3 it is possible to drive the light-emitting diode 1 without generating excessive heat, so that the light output is stable and in the desired range of few RLU (relative light units) of the typical detecting device for the bioluminescence of ATP.

(36) As shown in FIG. 2 the constant current converter 3 can be provided with a setting means 11 for setting the driving current through the light-emitting diode 1 by controlling the voltage V2 at the non-inverting input of the first operational amplifier 31.

(37) This can be done by either controlling the electrical power source 39 or by controlling the resistors R2 and R3, working as a voltage divider. For example, instead of the adjustable resistor R3 it is possible to provide a plurality of switchable resistors connected in parallel between the non-inverting input of the first operational amplifier 31 and ground and to control the respective switches in order to set a predetermined voltage as V2.

(38) A corresponding setting means 11 will preferably be provided with an interface for receiving setting signals from the outside, either by manual operation or by an electronic signal.

(39) FIG. 3 shows a further embodiment of the calibration device of the present invention.

(40) The calibration device of FIG. 3 differs from the previously described embodiment in so far as that a spring biased switch is provided on one end of the pen like casing 7. This switch is operated by closing the lid of a detecting device and will switch the voltage V2 applied to the first operational amplifier 31. That is, the calibration device of FIG. 3 will emit light only when mounted in the detecting device with the lid closed. This configuration will help to save energy of a battery, powering the calibration device, and will help to prevent any heating of the calibration device due to an unintended continuous operation over a long time. It will further make sure that the calibration is not affected by stray light from the environment.

(41) Finally, FIG. 4 shows a setting device for setting one or more calibration points for the calibration device according to any of the above embodiments.

(42) As shown in FIG. 4 the setting device comprises a light detector 51, like a photomultiplier. The light detector 51 is provided in a housing 57 equipped with means for receiving the calibration device as described before. The light detector 51 is arranged at a position so that the light detector 51 will receive the light emitted from the calibration device.

(43) This signal, outputted by the light detector 51 is delivered to a calibration point setting means 55 for further processing. The calibration point setting means 55 might be implemented as a computer.

(44) Additionally, the setting device of the invention is equipped with a controller 53, which is connected with the calibration device under examination.

(45) In the preferred embodiment additionally the controller 53 is connected with the calibration point setting means 55 as well. In this way, a feedback control is implemented. That is, the controller 53 will set the driving current to be applied to the light-emitting diode 1 of the calibration device. The light detector 51 will report the measured light amount to the calibration point setting means 55, which in turn will advise the controller 53, either to increase or decrease the driving current for the light-emitting diode 1, so that the calibration device will output a predetermined amount of light.

(46) The setting of the driving current for the calibration device is preferably done by and adjusting the voltage V2 at the noninverting input of the first operational amplifier 31.

(47) As an alternative to setting the driving current for the light-emitting diode 1 it will be possible to simply prepare a table displaying the relation between a respective calibration device and light detected by the light detector 51 while applying a driving current through the light-emitting diode 1.

(48) This table can be used to recalibrate values obtained during use by a detection device in to which the calibration device under examination is inserted.

(49) Although the invention has been described with reference to preferred embodiments, the present invention is not limited to these embodiments. For example, alternative configurations of the constant current generator 3 will be possible, as long as a reliable and constant small driving current in the claimed range can be obtained.