Deuterated fluorophores

Abstract

The present invention is generally directed to the synthesis and use of fluorophores. It is more specifically directed to the synthesis and use of deuterated fluorophores. In one case, the present invention provides a compound of the structure shown in FIG. 44.

Claims

1. A compound of the following structure: ##STR00058## wherein L is one of the following structures ##STR00059## wherein X is O, Si(CH.sub.3).sub.2 or C(CH.sub.3).sub.2; and, wherein R.sub.5, R.sub.7 and R.sub.8 are H, and wherein R.sub.6 is selected from H, COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), C(O)NH(CH.sub.2).sub.2O—(CH.sub.2).sub.2O(CH.sub.2).sub.6—Cl and ##STR00060##

2. The compound according to claim 1, wherein the compound is of the following structure: ##STR00061## wherein R.sub.5, R.sub.7 and R.sub.8 are H, and wherein R.sub.6 is selected from H, COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), C(O)NH(CH.sub.2).sub.2O—(CH.sub.2).sub.2O(CH.sub.2).sub.6—Cl and ##STR00062##

3. The compound according to claim 1, wherein the compound is of the following structure: ##STR00063## wherein R.sub.5, R.sub.7 and R.sub.8 are H, and wherein R.sub.6 is selected from H, COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), C(O)NH(CH.sub.2).sub.2O—(CH.sub.2).sub.2O(CH.sub.2).sub.6—Cl and ##STR00064##

4. The compound according to claim 2, wherein the compound is of the following structure: ##STR00065##

5. The compound according to claim 2, wherein the compound is of the following structure: ##STR00066##

6. The compound according to claim 2, wherein the compound is of the following structure: ##STR00067##

7. The compound according to claim 3, wherein the compound is of the following structure: ##STR00068##

8. The compound according to claim 3, wherein the compound is of the following structure: ##STR00069##

9. The compound according to claim 3, wherein the compound is of the following structure: ##STR00070##

10. The compound according to claim 4, wherein the compound is of the following structure: ##STR00071## wherein R.sub.6 is H, COOH, COO(alkyl), COO(aryl), or C(O)NH(CH.sub.2).sub.2O(CH.sub.2).sub.2O(CH.sub.2).sub.6—Cl.

11. The compound according to claim 5, wherein the compound is of the following structure: ##STR00072## wherein R.sub.6 is H, COOH, COO(alkyl), COO(aryl), or C(O)NH(CH.sub.2).sub.2O(CH.sub.2).sub.2O(CH.sub.2).sub.6—Cl.

12. The compound according to claim 6, wherein the compound is of the following structure: ##STR00073## wherein R.sub.6 is H, COOH, COO(alkyl), COO(aryl), or C(O)NH(CH.sub.2).sub.2O(CH.sub.2).sub.2O(CH.sub.2).sub.6—Cl.

13. The compound according to claim 7, wherein the compound is of the following structure: ##STR00074## wherein R.sub.6 is H, COOH, COO(alkyl), COO(aryl), or C(O)NH(CH.sub.2).sub.2O(CH.sub.2).sub.2O(CH.sub.2).sub.6—Cl.

14. The compound according to claim 8, wherein the compound is of the following structure: ##STR00075## wherein R.sub.6 is H, COOH, COO(alkyl), COO(aryl), or C(O)NH(CH.sub.2).sub.2O(CH.sub.2).sub.2O(CH.sub.2).sub.6—Cl.

15. The compound according to claim 9, wherein the compound is of the following structure: ##STR00076## wherein R.sub.6 is H, COOH, COO(alkyl), COO(aryl), or C(O)NH(CH.sub.2).sub.2O(CH.sub.2).sub.2O(CH.sub.2).sub.6—Cl.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 shows a Jablonski diagram for and excited state reactions of rhodamine dyes.

(2) FIG. 2 shows quantum yield values of polycyclic aromatic compounds 5-7 and their deuterated analogs.

(3) FIG. 3 shows the quantum yield value of porphyrin 8 and its deuterated analog.

(4) FIG. 4 shows quantum yield values of rhodamine 9 and its deuterated analog.

(5) FIG. 5 shows previous examples of fluorophores with deuterated N-alkyl groups 10-12.

(6) FIG. 6 shows synthesis of dyes 1, 14-22 and their deuterated analogs from fluorescein ditriflate 13 and their spectral properties.

(7) FIG. 7 shows absorbance (abs) and fluorescence emission (em) spectra of 1 and 14 in 10 mM HEPES pH 7.3.

(8) FIG. 8 shows photobleaching of 1 and 14 in 10 mM HEPES pH 7.3.

(9) FIG. 9 shows a comparison of the bleaching time constants (s) of different rhodamines (1, 15, 17) and their deuterated analogs (14, 16, 18).

(10) FIG. 10 shows a comparison of the intrinsic brightness (ε×Φ) of rhodamine dyes 1, 14-22 in 10 mM HEPES pH 7.3.

(11) FIG. 11 shows the absorbance (abs) and fluorescence emission (em) spectra of 17 and 18 in 10 mM HEPES pH 7.3.

(12) FIG. 12 shows the absorbance (abs) and fluorescence emission (em) spectra of 19 and 20 in 10 mM HEPES pH 7.3.

(13) FIG. 13 shows the absorbance (abs) and fluorescence emission (em) spectra of 21 and 22 in 10 mM HEPES pH 7.3.

(14) FIG. 14 shows the photobleaching of 17 and 18 in 10 mM HEPES pH 7.3.

(15) FIG. 15 shows the photobleaching of 15 and 16 in 10 mM HEPES pH 7.3.

(16) FIG. 16 shows the absorbance (abs) and fluorescence emission (em) spectra of 15 and 16 in 10 mM HEPES pH 7.3.

(17) FIG. 17 shows the relative singlet oxygen quantum yield (.sup.1O.sub.2) of 15 and 16.

(18) FIG. 18 shows the normalized fluorescence emission spectra and zoom-in of 15 taken periodically during photobleaching experiments showing 9 nm blue-shift due to dealkylation.

(19) FIG. 19 shows the normalized fluorescence emission spectra and zoom-in of 16 taken periodically during photobleaching experiments showing 4 nm blue-shift due to dealkylation.

(20) FIG. 20 shows the chemical structures of HaloTag ligand 23 and deuterated analog 24.

(21) FIG. 21 shows the fluorescence quantum yield of HaloTag-bound 23 and deuterated analog 24.

(22) FIG. 22 shows the fluorescence lifetime of histone H2B-HaloTag-bound 23 and deuterated analog 24 in live cells measured using FLIM.

(23) FIG. 23 shows the relative singlet oxygen quantum yield (.sup.1O.sub.2) of HaloTag-bound 23 and deuterated analog 24.

(24) FIG. 24 shows the image of the nucleus of a mammalian cell expressing histone H2B-HaloTag fusion and incubated with 23 (10 pM, 30 min), then washed for 30 minutes prior to fixation and imaging.

(25) FIG. 25 shows the image of the nucleus of a mammalian cell expressing histone H2B-HaloTag fusion and incubated with 24 (10 pM, 30 min), then washed for 30 minutes prior to fixation and imaging.

(26) FIG. 26 shows the chemical structure of deuterated pyrrolidinyl HaloTag ligand 25.

(27) FIG. 27 shows the image of the nucleus of a mammalian cell expressing histone H2B-HaloTag fusion and incubated with 25 (10 pM, 30 min), then washed for 30 minutes prior to fixation and imaging.

(28) FIG. 28 shows the photostability (track lengths, s) of single molecules of HaloTag ligands 23-25 in live-cell single-particle tracking experiments.

(29) FIG. 29 shows the brightness (kcps) of single molecules of HaloTag ligands 23-25 in live-cell single-particle tracking experiments.

(30) FIG. 30 shows the chemical structures and spectral properties of dyes 26-33 in 10 mM HEPES pH 7.3.

(31) FIG. 31 shows the comparison of the intrinsic brightness (ε×Φ) of rhodamine dyes 26-33 in 10 mM HEPES pH 7.3.

(32) FIG. 32 shows the chemical structures of carborhodamine HaloTag ligands 34 and 35.

(33) FIG. 33 shows the chemical structures of Si-rhodamine HaloTag ligand 36 and deuterated analog 37.

(34) FIG. 34 shows the fluorescence quantum yield of HaloTag-bound 36 and deuterated analog 37.

(35) FIG. 35 shows the fluorescence lifetime of histone H.sub.12B-HaloTag-bound 36 and deuterated analog 37 in live cells measured using FLIM.

(36) FIG. 36 shows the relative singlet oxygen quantum yield (1O2) of HaloTag-bound 36 and deuterated analog 37.

(37) FIG. 37 shows the image of the nucleus of a mammalian cell expressing histone H2B-HaloTag fusion and incubated with 36 (10 pM, 30 min), then washed for 30 minutes prior to fixation and imaging.

(38) FIG. 38 shows the image of the nucleus of a mammalian cell expressing histone H2B-HaloTag fusion and incubated with 37 (10 pM, 30 min), then washed for 30 minutes prior to fixation and imaging.

(39) FIG. 39 shows the chemical structure of deuterated pyrrolidinyl HaloTag ligand 38.

(40) FIG. 40 shows the image of the nucleus of a mammalian cell expressing histone H2B-HaloTag fusion and incubated with 38 (10 pM, 30 min), then washed for 30 minutes prior to fixation and imaging.

(41) FIG. 41 shows the photostability (track lengths, s) of single molecules of HaloTag ligands 36-38 in live-cell single-particle tracking experiments.

(42) FIG. 42 shows the brightness (kcps) of single molecules of HaloTag ligands 36-38 in live-cell single-particle tracking experiments.

(43) FIG. 43 shows the chemical structures and spectral properties of dyes 39-44 in 10 mM HEPES pH 7.3.

(44) FIG. 44 shows a general structure for a compound of the present invention.

(45) FIG. 45 shows further structures for a compound of the present invention.

(46) FIG. 46 shows further structures for a compound of the present invention.

(47) FIG. 47 shows further structures for a compound of the present invention.

(48) FIG. 48 shows further structures for a compound of the present invention.

(49) FIG. 49 shows further structures for a compound of the present invention.

(50) FIG. 50 shows further structures for a compound of the present invention.

(51) FIG. 51 shows further structures for a compound of the present invention.

(52) FIG. 52 shows further structures for a compound of the present invention.

(53) FIG. 53 shows further structures for a compound of the present invention.

(54) FIG. 54 shows further structures for a compound of the present invention.

(55) FIG. 55 shows further structures for a compound of the present invention.

(56) FIG. 56 shows further structures for a compound of the present invention.

(57) FIG. 57 shows further structures for a compound of the present invention.

(58) FIG. 58 shows further structures for a compound of the present invention.

(59) FIG. 59 shows further structures for a compound of the present invention.

(60) FIG. 60 shows further structures for a compound of the present invention.

(61) FIG. 61 shows further structures for a compound of the present invention.

(62) FIG. 62 shows further structures for a compound of the present invention.

(63) FIG. 63 shows further structures for a compound of the present invention.

(64) FIG. 64 shows further structures for a compound of the present invention.

(65) FIG. 65 shows further structures for a compound of the present invention.

(66) FIG. 66 shows further structures for a compound of the present invention.

(67) FIG. 67 shows further structures for a compound of the present invention.

(68) FIG. 68 shows a scheme for synthesizing compounds of the present invention.

(69) FIG. 69 shows a further scheme for synthesizing compounds of the present invention.

(70) FIG. 70 shows a further scheme for synthesizing compounds of the present invention.

DETAILED DESCRIPTION OF THE INVENTION

(71) Rhodamine dyes such as tetramethylrhodamine (TMR, 1, FIG. 1) remain in wide use due to their excellent brightness, superb photostability, and broad spectral range..sup.1-3 The photophysics of rhodamines are well understood due to their importance as biological probes and laser dyes..sup.4 Absorption of a photon excites the TMR molecule from the ground state (1-S.sub.0) ultimately to the first excited state (1-S.sub.1). After excitation, the molecule can relax back to S.sub.0 through a variety of processes. Emission of a photon (fluorescence) competes with nonradiative decay pathways such as twisted internal charge transfer (TICT) where electron transfer from the aniline nitrogen to the xanthene system gives a charge-separated species (1-TICT) that rapidly decays back to the ground state (1-S.sub.0) without emitting a photon..sup.5 This process competes with fluorescence, thereby decreasing fluorescence quantum yield (<D). Alternatively, the excited dye can undergo intersystem crossing to the first triplet excited state (1-T.sub.1) where it can sensitize singlet oxygen (.sup.1O.sub.2), returning to the ground state (1-S.sub.0). The resulting .sup.1O.sub.2 can then react with the ground state of the dye, oxidizing the aniline nitrogen to radical cation (2), which can undergo deprotonation to a carbon-centered radical (3) that ultimately results in dealkylation of the dye to form trimethylrhodamine (4; FIG. 1)..sup.4 This process results in a blue-shift in absorption (λ.sub.max) and emission (em) maxima, which can complicate multicolor experiments and is a prelude to additional dealkylation and irreversible photobleaching steps.

(72) Both of these undesirable processes—TICT and dealkylation—can be mitigated through modifications in the chemical structure of the dye. Since both involve oxidation of the aniline nitrogen, methods to increase the ionization potential of this atom can improve both brightness and photostability. It was discovered that replacing the N,N-dimethylamino groups with 4-membered azetidine rings further improved the brightness and photostability of rhodamine and other dyes. This was likely due, in part, to the higher ionization potential of azetidines, which would make both the TICT and dealkylation pathways (FIG. 1) less favorable.

(73) Another strategy to increase brightness and photostability of small-molecule fluorophores such as 1 was envisioned by replacing the hydrogen (H) atoms on the N-alkyl groups with deuterium (D). Deuterated alkylamines exhibit higher ionization potentials relative to their hydrogen-containing analogs,.sup.6 suggesting that deuteration could decrease the efficiency of the TICT process and therefore increase quantum yield. This higher ionization potential could also slow the initial electron abstraction step (i.e., 1.fwdarw.2, FIG. 1) and the stronger C-D bond could slow the rate of deprotonation (i.e., 2.fwdarw.3, FIG. 1), together decreasing the efficiency of the dealkylation step.

(74) Deuteration has long been proposed as a means to increase Φ.sup.7 and many fluorophores show improvements in brightness and photostability in deuterated solvents. Nevertheless, prior examples of deuterated dyes are rare and deuteration typically has a negative or neutral effect on Φ as demonstrated for simple polycyclic aromatic compounds (5-7; FIG. 2).sup.8-10, porphyrins (8; FIG. 3), and rhodamines deuterated at other positions (9; FIG. 4). It was therefore nonobvious that deuteration of the N-alkyl groups would improve the properties of rhodamines. Furthermore, the only previous example of fluorophores with N-alkyl groups deuterated alpha to the nitrogen are a partially deuterated tetraethylrhodamine-d.sub.10 (i.e., rhodamine B-d.sub.10, 10; FIG. 5) and the fully deuterated tetraethylrhodamine-d.sub.20 (i.e., rhodamine B-d.sub.20, 11; FIG. 5), which were prepared to study fragmentation of rhodamines in mass spectrometry experiments,.sup.12 and the N,N-dimethylaminocoumarin-d.sub.6 compound 12 (FIG. 5) which was a control for excited-state proton transfer experiments..sup.13 The spectroscopic properties of fluorophores 10-12 were not reported so the effect of deuterated alkylamino groups on fluorescence was unknown.

(75) The hypothesis that deuteration of N-alkyl groups would improve brightness and photostability was tested by synthesizing a series of rhodamine dyes and their deuterated counterparts using a cross-coupling approach starting from fluorescein ditriflate (13; FIG. 6)..sup.14 We first compared TMR (1) and its deuterated analog 14, finding remarkably similar absorption maximum (λ.sub.max) and fluorescence emission maximum (λ.sub.em; FIG. 6) for the two dyes with no change in the shape of the absorption peak (FIG. 7). Deuteration did affect the brightness and photostability of the dye, however, with 14 showing a ˜20% increase in both the extinction coefficient at λ.sub.max (ε) and Φ compared to 1 (FIG. 6) and slower rate of photobleaching (FIG. 8, FIG. 9). Based on this result with TMR (1) other matched pairs of rhodamine dyes with H- or D-containing cyclic N-alkyl groups were tested (15-22, FIG. 6). Like the TMR compounds 1 and 14, increases in ε and Φ for the deuterated pyrrolidine-, piperidine-, and morpholine-containing rhodamines 18, 20, and 22 were observed compared to the parent compounds 17, 19, and 21 (FIG. 6, FIG. 10) with no change in spectral shape (FIG. 11, FIG. 12, FIG. 13); the deuterated pyrrolidine 18 also showed improved in vitro photostability (FIG. 9, FIG. 14). Interestingly, the deuterated azetidine-containing rhodamine (16) showed no improvement in ε, Φ, or photostability over the parent nondeuterated 15 (‘Janelia Fluor’ 549,.sup.5 JF.sub.549; FIG. 6, FIG. 9, FIG. 10, FIG. 15) although it showed similar spectra (FIG. 16). This result suggests that the azetidine and deuterium substitutions suppress the same nonradiative pathways (e.g., TICT) and are therefore not additive. Interestingly, the deuterium substitution did elicit a significantly lower singlet oxygen (.sup.1O.sub.2) quantum yield (FIG. 17), suggesting that deuterium modulates either the intersystem crossing to T.sub.1 or relaxation from T.sub.1 (FIG. 1). Importantly, both the deuterated azetidine and deuterated pyrrolidine containing dyes 18 and 20 showed higher ‘chromostability’ during bleaching compared to nondeuterated compounds 15 and 17 (FIG. 18, FIG. 19), which indicates a higher resistance to the undesirable dealkylation pathway (FIG. 1) and concomitant blue spectral shift.

(76) These dyes were then tested as protein conjugates in vitro and in living cells. As both azetidine compounds showed high ε and Φ, the HaloTag.sup.15 ligands of the azetidinyl-rhodamines 15 and 16 (23 and 24; FIG. 20) were synthesized first. The fluorescence properties of azetidinyl compounds 23 and 24 attached to HaloTag protein were compared, finding that that the conjugates of the deuterated dye 24 showed higher fluorescence quantum yield in vitro (FIG. 21) and longer fluorescence lifetime in cells (T; FIG. 22) compared to the nondeuterated 23. This result suggests that deuteration suppresses a protein-bound-specific mode of nonradiative decay. Like the free dyes, deuteration also significantly suppressed .sup.1O.sub.2 generation (FIG. 23). Both ligands could label HaloTag-histone H2B fusions in living cells (FIG. 24, FIG. 25).

(77) Based on the high brightness of the deuterated pyrrolidine-containing rhodamine 18 (FIG. 6) the HaloTag ligand of this compound (25; FIG. 26) was also synthesized, which was an excellent live-cell label (FIG. 27). HaloTag ligand 24-26 in live-cell single-particle tracking experiments using sparsely expressed Sox2-HaloTag fusions were compared..sup.16 In line with the in vitro bleaching experiments on the free dyes (FIG. 9, FIG. 14, FIG. 15), deuteration of the azetidine showed no improvement in photostability in cells (i.e., average track length of individual molecules; FIG. 28), but the deuterated pyrrolidine rhodamine ligand 25 did show significantly longer tracks compared to azetidinyl dyes 23 and 24. Deuteration did elicit a higher brightness (i.e., photons/s, FIG. 29) with both 24 and 25 conjugates emitting more photons per unit time compared to conjugates of 23 under equivalent imaging conditions.

(78) This modification was then applied to other rhodamine analogs, focusing on the azetidine and pyrrolidine modifications based on the high brightness observed for the rhodamines 16 and 18 (FIG. 6). For both the azetidinyl and pyrrolidinyl carborhodamines.sup.17 26 and 28, substantial increases in both Φ and ε were observed when the cyclic amines were deuterated to give 27 and 29 (FIG. 30, FIG. 31). The HaloTag ligands of compounds 27 and 29 (34 and 35, FIG. 32) were prepared. The Si-rhodamines 30 and 32 and their deuterated analogs 31 and 33 (FIG. 30) were then examined. As with the rhodamine series, deuteration of the azetidinyl rhodamine 30 to give 31 did not elicit a large increase in fluorescence quantum yield (0), although the extinction coefficient in water (s) was modestly increased, yielding an increase in intrinsic brightness (FIG. 31). Deuteration of the pyrrolidine-containing rhodamine 32 to give 33 did elicit a substantial increase in both 0 and E, in line with the rhodamine series (FIG. 6).

(79) The HaloTag ligands of the azetidinyl Si-rhodamine compounds (36 and 37, FIG. 33) were then synthesized, and the effect of deuteration in vitro and in living cells was measured. Like the analogous rhodamine compounds, the deuterated azetidinyl dye 37 showed a substantial increase in Φ compared to 36 when attached to the HaloTag (FIG. 34); compound 37 also showed increased fluorescence lifetime (τ) as the HaloTag conjugate inside live cells (FIG. 35) and lower 102 generation (FIG. 36). Both these dyes were suitable labels for HaloTag expressed in cells (FIG. 37, FIG. 38). The deuterated pyrrolidinyl Si-rhodamine HaloTag ligand (38, FIG. 39) was also prepared, which showed improved cellular labeling (FIG. 40) relative to azetidinyl compounds 36 and 37 (cf. FIG. 37 and FIG. 38). In live-cell single-molecule experiments it was discovered that the HaloTag conjugates of deuterated dyes 37 and 38 were both more photostable than the conjugates of nondeuterated 36, with deuterated pyrrolidine Si-rhodamine 38 showing the longest average single-molecule track length (FIG. 41). Likewise, the conjugates of the deuterated dyes exhibited higher brightness with 38 showing the highest photons/s (FIG. 42).

(80) The deuterium substitution was applied to other dyes beyond tetramethylrhodamine analogs. The coumarin scaffold was explored first, synthesizing the azetidinyl coumarins 39 and 40 and the pyrrolidinyl pair 41 and 42 (FIG. 43). Consistent with the other dyes, the spectral properties of the deuterated azetidine compound 40 was similar to the parent 39 with similar c and a small decrease in 0. For the pyrrolidinyl compounds the differences were more pronounced, with deuterated dye 42 showing significantly higher 0 compared to 41; the s for the two dyes was equivalent (FIG. 43). We also synthesized the pyrrolidinyl oxazine compound 43 and deuterated congener 44. Like the other pyrrolidine-containing dyes, the deuterium substitution caused a substantial increase in Φ.

(81) In conclusion, deuteration of the N-alkyl groups of rhodamine dyes elicits substantial improvements in performance. For standard tetraalkyl dyes 1, 14, 17-22, increases in both extinction coefficient and quantum yields (FIG. 6, FIG. 10) are seen. For the already optimized azetidine containing dyes (15-16), deuteration did not further improve brightness in vitro, but it did result in suppressed .sup.1O.sub.2 generation (FIG. 17) and slower dealkylation (FIG. 18, FIG. 19) as well as improved brightness and photostability as the HaloTag conjugates (FIG. 21, FIG. 22). In addition, the deuterated pyrrolidinyl compound 18 showed equivalent brightness to the azetidinyl dyes in vitro (FIG. 6, FIG. 30) and its derivatives exhibited superior brightness and photostability inside cells (FIG. 41, FIG. 42). These improvements due to deuterium substitution were generalizable to carborhodamines (e.g., 29, FIG. 30), coumarins (e.g., 42, FIG. 43), oxazines (e.g., 44, FIG. 43). Overall, this represents a new strategy for improving the performance of fluorophores, especially in the live-cell environment.

(82) References: (1) Lavis, L. D.; Raines, R. T. ACS Chem. Biol. Bright ideas for chemical biology. 2008, 3, 142-155. (2) Beija, M.; Afonso, C. A. M.; Martinho, J. M. G. Chem. Soc. Rev. Synthesis and applications of rhodamine derivatives as fluorescent probes. 2009, 38, 2410-2433. (3) Lavis, L. D.; Raines, R. T. ACS Chem. Biol. Bright building blocks for chemical biology. 2014, 9, 855-866. (4) Zheng, Q.; Lavis, L. D. Curr. Opin. Chem. Biol. Development of photostable fluorophores for molecular imaging. 2017, 39, 32-38. (5) Grimm, J. B.; English, B. P.; Chen, J.; Slaughter, J. P.; Zhang, Z.; Revyakin, A.; Patel, R.; Macklin, J. J.; Normanno, D.; Singer, R. H.; Lionnet, T.; Lavis, L. D. Nat. Methods A general method to improve fluorophores for live-cell and single-molecule microscopy. 2015, 12, 244-250. (6) Hull, L. A.; Davis, G. T.; Rosenblatt, D. H.; Williams, H. K. R.; Weglein, R. C. J. Am. Chem. Soc. Oxidations of Amines. III. Duality of Mechanism in the Reaction of Amines with Chlorine Dioxide. 1967, 89, 1163-1170. (7) Turro, N. J.; Ramamurthy, V.; Scaiano, J. C. Modern Molecular Photochemistry of Organic Molecules; University Science Books, 2010. (8) Dawson, W. R.; Windsor, M. W. J. Phys. Chem. Fluorescence yields of aromatic compounds. 1968, 72, 3251-3260. (9) Kolmakov, K.; Belov, V. N.; Bierwagen, J.; Ringemann, C.; Muller, V.; Eggeling, C.; Hell, S. W. Chem. Eur. J. Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy. 2010, 16, 158-166. (10) Frampton, M. J.; Accorsi, G.; Armaroli, N.; Rogers, J. E.; Fleitz, P. A.; McEwan, K. J.; Anderson, H. L. Org. Biomol. Chem. Synthesis and near-infrared luminescence of a deuterated conjugated porphyrin dimer for probing the mechanism of non-radiative deactivation. 2007, 5, 1056-1061. (11) Clemen, M.; Gernert, C.; Peters, J.; Grotemeyer, J. Eur. J. Mass Spectrom. Fragmentation reactions of labeled and unlabeled rhodamine B in a high-resolution Fourier transform ion cyclotron resonance mass spectrometer. 2013, 19, 135-139. (12) Peters, J.; Clemen, M.; Grotemeyer, J. Anal. Bioanal. Chem. Fragmentation of deuterated rhodamine B derivates by laser and collisional activation in an FT-ICR mass spectrometer. 2013, 405, 7061-9. (13) Pal, H.; Nagasawa, Y.; Tominaga, K.; Yoshihara, K. J. Phys. Chem. Deuterium isotope effect on ultrafast intermolecular electron transfer. 1996, 100, 11964-11974. (14) Grimm, J. B.; Lavis, L. D. Org. Lett. Synthesis of rhodamines from fluoresceins using Pd-catalyzed C—N cross-coupling. 2011, 13, 6354-7. (15) Los, G. V.; Encell, L. P.; McDougall, M. G.; Hartzell, D. D.; Karassina, N.; Zimprich, C.; Wood, M. G.; Learish, R.; Ohana, R. F.; Urh, M. ACS Chem. Biol. HaloTag: A novel protein labeling technology for cell imaging and protein analysis. 2008, 3, 373-382. (16) Liu, Z.; Legant, W. R.; Chen, B. C.; Li, L.; Grimm, J. B.; Lavis, L. D.; Betzig, E.; Tjian, R. Elife 3D imaging of Sox2 enhancer clusters in embryonic stem cells. 2014, 3, e04236. (17) Grimm, J. B.; Sung, A. J.; Legant, W. R.; Hulamm, P.; Matlosz, S. M.; Betzig, E.; Lavis, L. D. ACS Chem. Biol. Carbofluoresceins and carborhodamines as scaffolds for high-contrast fluorogenic probes. 2013, 8, 1303-1310.

(83) “Alkyl” refers to an alkane missing one hydrogen and having the general formula C.sub.nH.sub.2n+1. Examples of lower alkyls (C1-C5) include: methyl; ethyl; propyl; butyl; and pentyl. Other, nonlimiting examples of alkyls are: hexyl; heptyl; octyl; nonyl; and decyl.

(84) “Deuterated”, as in “deuterated compound, refers to a synthesized compound that has significantly more deuterium included than would be predicted by natural isotopic abundance. Typically, when “D”, designating deuterium, is used instead of “H”, designating hydrogen that is more than 98% hydrogen-1, in a chemical structure, it refers to hydrogen that is more than 50% deuterium. In certain cases, it refers to hydrogen that is more than 60%, more than 70%, more than 80%, more than 90%, more than 95%, more than 97.5%, more than 98.0% or more than 98.5% deuterium.

(85) “Substituted alkyl” refers to an alkyl where one or more hydrogen atoms have been replaced with a different substituent. Nonlimiting examples of such substituents include: alkyl; alkenyl; alkynyl; cycloalkyl; cycloalkenyl; heterocycloalkyl; heterocycloalkenyl; aromatic group; heteroaromatic group; OH; O-alkyl; NH.sub.2; NH-alkyl; SH; CN; NO.sub.2; CF.sub.3; C(O)H; C(O)-alkyl; CO.sub.2H; CO.sub.2-alkyl; OC(O)CH.sub.3.

(86) “Aryl” refers to a cyclic or multi-cyclic, planar molecule with a ring of resonance bonds that exhibit more stability than other geometric or connective arrangements with the same set of atoms. Nonlimiting examples of aromatic groups include: phenyl; naphthyl; anthracenyl; and phenanthrenyl.

(87) “Substituted aryl” refers to an aromatic group where one or more hydrogen atoms have been replaced with a different substituent. Nonlimiting examples of such substituents include: alkyl; alkenyl; alkynyl; cycloalkyl; cycloalkenyl; heterocycloalkyl; heterocycloalkenyl; aromatic group; heteroaromatic group; OH; O-alkyl; NH.sub.2; NH-alkyl; SH; CN; NO.sub.2; CF.sub.3; C(O)H; C(O)-alkyl; CO.sub.2H; CO.sub.2-alkyl; OC(O)CH.sub.3.

(88) “Cycloalkyl” refers to a cycloalkane missing one hydrogen and having the general formula C.sub.nH.sub.2n+1. Nonlimiting examples of cycloalkyls include: cyclopropyl; cyclobutyl; cyclopentyl; cyclohexyl; cycloheptyl; cyclooctyl; cyclononyl; and cyclodecyl.

(89) “Substituted cycloalkyl” refers to a cycloalkyl where one or more hydrogen atoms have been replaced with a different substituent. Nonlimiting examples of such substituents include: alkyl; alkenyl; alkynyl; cycloalkyl; cycloalkenyl; heterocycloalkyl; heterocycloalkenyl; aromatic group; heteroaromatic group; OH; O-alkyl; NH.sub.2; NH-alkyl; SH; CN; NO.sub.2; CF.sub.3; C(O)H; C(O)-alkyl; CO.sub.2H; CO.sub.2-alkyl; OC(O)CH.sub.3.

(90) “HaloTag” refers to a protein tag including a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands. The synthetic ligands comprise a chloroalkane linker attached to a variety of molecules. Nonlimiting examples of such molecules include: biotin; fluorescent dyes (e.g., Coumarin, Oregon Green, Alexa Fluor 488, diAcFAM and TMR); affinity handles; and solid surfaces. See, for example, Los et al., “A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis”, ACS Chem. Biol. 2008, 3, 373-382, which is incorporated-by-reference into this document for all purposes.

(91) The present invention provides deuterated fluorophores. FIG. 44 shows a general structure for such a deuterated fluorophore, where the substituents of the compound are as follows: R.sub.1 is independently selected from halogen, H, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, N(alkyl).sub.3, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl or where the R.sub.1 and R.sub.1′ substituents, taken together with the carbon atoms to which they are bonded, form a substituted or unsubstituted cycloalkyl ring containing 3, 4, 5, 6, 7, 8, or 9 carbon atoms; R.sub.1′ is independently selected from halogen, H, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, N(alkyl).sub.3, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl and only one of R.sub.1 and R.sub.1′ can be D when X is CF.sub.3, and only one of R.sub.1 and R.sub.1′ can be CD.sub.3 when Q is O; R.sub.2, R.sub.3 and R.sub.4 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, N(alkyl).sub.3, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; Q is selected from C(alkyl).sub.2, NH, N(alkyl), O, S, Si(alkyl).sub.2, SO.sub.2, P(O)(alkyl) P(O)(aryl) and Se; W is selected from C and N; X is selected from H, alkyl, substituted alkyl (e.g., CF.sub.3), aryl, substituted aryl, halogen, CN, OH, O(alkyl), SH, S(alkyl), S(aryl), NH.sub.2, NH.sub.2, NH(alkyl), N(alkyl).sub.2, N(alkyl).sub.3, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, COOH, COO(alkyl), COO(aryl), PO.sub.3H.sub.2 and SO.sub.3H; Y is selected from H, C(alkyl).sub.2, NH.sub.2, N(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, O and S; Z is selected from H, halogen, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, N(alkyl).sub.3, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl, and substituted alkyl or Z and Y, taken together with the carbon atoms to which they are bonded, form a substituted or unsubstituted cycloalkyl ring containing 4, 5, 6, 7 or 8 ring carbon atoms, or Z and Y, taken together with the carbon atoms to which they are bonded, form a substituted of unsubstituted aryl ring.

(92) FIG. 45 shows four structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3 and R.sub.4 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, N(alkyl).sub.3, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; W is selected from C and N; X is selected from H, alkyl, substituted alkyl, aryl, substituted aryl, halogen, CN, OH, O(alkyl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, N(alkyl).sub.3, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), PO.sub.3H.sub.2 and SO.sub.3H, but X is not CF.sub.3; Z is selected from H, halogen, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl, and substituted alkyl.

(93) FIG. 46 shows four structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3 and R.sub.4 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, N(alkyl).sub.3, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; W is selected from C and N; X is selected from H, alkyl, substituted alkyl (e.g., CF.sub.3), aryl, substituted aryl, halogen, CN, OH, O(alkyl), SH, S(alkyl), S(aryl), amine, NO.sub.2, CHO, COOH, COO(alkyl), COO(aryl), PO.sub.3H.sub.2 and SO.sub.3H; Z is selected from H, halogen, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(94) FIG. 47 shows four structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3 and R.sub.4 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, C(O)(alkyl), C(O)(aryl), CHO, COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; W is selected from C and N; X is selected from H, alkyl, substituted alkyl (e.g., CF.sub.3), aryl, substituted aryl, halogen, CN, OH, O(alkyl), SH, S(alkyl), S(aryl), amine, NO.sub.2, CHO, COOH, COO(alkyl), COO(aryl), PO.sub.3H.sub.2 and SO.sub.3H; NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(95) FIG. 48 shows four structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3 and R.sub.4 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; W is selected from C and N; X is selected from H, alkyl, substituted alkyl (e.g., CF.sub.3), aryl, substituted aryl, halogen, CN, OH, O(alkyl), SH, S(alkyl), S(aryl), amine, NO.sub.2, CHO, COOH, COO(alkyl), COO(aryl), PO.sub.3H.sub.2 and SO.sub.3H; Z is selected from H, halogen, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(96) FIG. 49 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(97) FIG. 50 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(98) FIG. 51 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(99) FIG. 52 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; R.sub.12 and R.sub.13 are independently selected from CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, aryl and substituted aryl.

(100) FIG. 53 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(101) FIG. 54 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(102) FIG. 55 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; R.sub.12 and R.sub.13 are independently selected from CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, aryl and substituted aryl.

(103) FIG. 56 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; R.sub.12 and R.sub.13 are independently selected from CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, aryl and substituted aryl.

(104) FIG. 57 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(105) FIG. 58 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(106) FIG. 59 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(107) FIG. 60 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; R.sub.12 and R.sub.13 are independently selected from CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, aryl and substituted aryl.

(108) FIG. 61 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; R.sub.12 and R.sub.13 are independently selected from CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, aryl and substituted aryl.

(109) FIG. 62 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11, are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(110) FIG. 63 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl.

(111) FIG. 64 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; R.sub.12 and R.sub.13 are independently selected from CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, aryl and substituted aryl.

(112) FIG. 65 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; R.sub.12 and R.sub.13 are independently selected from CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, aryl and substituted aryl.

(113) FIG. 66 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; R.sub.12 and R.sub.13 are independently selected from CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, aryl and substituted aryl.

(114) FIG. 67 shows two structures for deuterated fluorophores according to the present invention. Substituents for those structures are: R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, R.sub.8, R.sub.9, R.sub.10 and R.sub.11 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), N.sub.3, NH.sub.2, NH(alkyl), N(alkyl).sub.2, NH(aryl), NH(aryl).sub.2, NO.sub.2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl), COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), PO.sub.3H.sub.2, SO.sub.3H, alkyl and substituted alkyl, aryl and substituted aryl, alkenyl and substituted alkenyl; R.sub.12 and R.sub.13 are independently selected from CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, aryl and substituted aryl.

(115) The synthesis of hydrogen-1 analogs of rhodamine and coumarin derivatives has been reported. See, for example, Beija, M.; Alfonso, C. A. M.; Martinho, J. M. G. Chem. Soc. Rev. Synthesis and applications of rhodamine derivatives as fluorescent probes. 2009, 38, 2410-2433 (rhodamine derivatives) and Vekariya, R; Patel, H. Syn. Comm. Recent advances in the synthesis of coumarin derivatives via Knoevenagel Condensation: A review. 2014, 44, 2756-2788 (coumarin derivatives).

(116) Deuterated fluorophores according to the present invention can be synthesized using any suitable method. One synthetic method involves a cross-coupling approach. See, for example, Grimm, J. B.; Lavis, L. D. Org. Lett. Synthesis of rhodamines from fluoresceins using Pd-catalyzed C—N cross-coupling. 2011, 13, 6354-6357. Deuterated cross-coupling compounds such as pyrrolidine-d.sub.8 and piperidine-d.sub.11 can be purchased, e.g., Sigma-Aldrich, or synthesized using suitable methods, e.g. Atzrodt, J.; Derdau, V.; Holla, W.; Beller, M.; Neubert, L.; Michalik, D. European Patent Application EP2714624A1. Process for the preparation of deuterated compounds containing n-alkyl groups. 2012, and references therein. FIGS. 68-70 show schemes for synthesizing compounds of the present invention.

(117) Deuterated fluorophores according to the present invention can be used for any suitable purpose. Nonlimiting examples of such use include use as/for: a dye; fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.

EXPERIMENTALS

Example 1. 2-(3,6-Bis(bis(methyl-d.SUB.3.)amino)xanthylium-9-yl)benzoate

(118) ##STR00003##

(119) A vial was charged with fluorescein ditriflate (Grimm, J. B.; Lavis, L. D. Org. Lett. 2011, 13, 6354-6357; 150 mg, 0.251 mmol), dimethyl-d.sub.6-amine hydrochloride (52.9 mg, 0.604 mmol, 2.4 eq), Pd.sub.2dba.sub.3 (23.0 mg, 25.1 μmol, 0.1 eq), XPhos (36.0 mg, 75.4 μmol, 0.3 eq), and Cs.sub.2CO.sub.3 (393 mg, 1.21 mmol, 4.8 eq). The vial was sealed and evacuated/backfilled with nitrogen (3×). Dioxane (1.5 mL) was added, and the reaction was flushed again with nitrogen (3×). The reaction was then stirred at 100° C. for 4 h. It was subsequently cooled to room temperature, diluted with MeOH, deposited onto Celite, and concentrated to dryness. Purification by silica gel chromatography (0-10% MeOH (2 M NH.sub.3)/CH.sub.2Cl.sub.2, linear gradient; dry load on Celite) followed by reverse phase HPLC (10-50% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive) afforded the title compound (92 mg, 71%, TFA salt) as a dark red solid. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.37-8.32 (m, 1H), 7.86 (td, J=7.5, 1.5 Hz, 1H), 7.80 (td, J=7.6, 1.5 Hz, 1H), 7.44-7.38 (m, 1H), 7.15 (d, J=9.5 Hz, 2H), 7.05 (dd, J=9.5, 2.5 Hz, 2H), 6.97 (d, J=2.5 Hz, 211); Analytical HPLC: t.sub.R=10.5 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.24H.sub.11D.sub.12N.sub.2O.sub.3 [M+H].sup.+ 399.2456, found 399.2454.

Example 2. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)xanthylium-9-yl)benzoate

(120) ##STR00004##

(121) The title compound (81%, dark red solid) was prepared from fluorescein ditriflate and azetidine-2,2,3,3,4,4-d.sub.6 hydrochloride (Helal, C. J.; Chappie, T. A.; Humphrey, J. M. Int. Pat. Appl. WO 2012/168817 A1, Dec. 13, 2012) according to the procedure described for Example 1. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.09-8.06 (m, 1H), 7.64 (td, J=7.5, 1.6 Hz, 11H), 7.59 (td, J=7.4, 1.6 Hz, 11H), 7.22-7.19 (m, 1H), 7.18 (d, J=9.2 Hz, 2H), 6.54 (dd, J=9.2, 2.2 Hz, 2H), 6.46 (d, J=2.2 Hz, 2H); Analytical HPLC: t.sub.R=11.3 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.26H.sub.11D.sub.12N.sub.2O.sub.3 [M+H].sup.+ 423.2456, found 423.2454.

Example 3. 2-(3,6-Bis(pyrrolidin-1-yl-d.SUB.8.)xanthylium-9-yl)benzoate

(122) ##STR00005##

(123) The title compound (76%, dark red-purple solid) was prepared from fluorescein ditriflate and pyrrolidine-2,2,3,3,4,4,5,5-d.sub.8 according to the procedure described for Example 1. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.11-8.07 (m, 11H), 7.65 (td, J=7.5, 1.6 Hz, 11H), 7.60 (td, J=7.4, 1.6 Hz, 1H), 7.26 (d, J=9.3 Hz, 2H), 7.25-7.22 (m, 1H), 6.85 (dd, J=9.3, 2.4 Hz, 2H), 6.75 (d, J=2.3 Hz, 2H); Analytical HPLC: t.sub.R=12.4 min, XX % purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.28H.sub.11D.sub.16N.sub.2O.sub.3 [M+H].sup.+ 455.3020, found 455.3018.

Example 4. 2-(3,6-Bis(piperidin-1-yl-d.SUB.10.)xanthylium-9-yl)benzoate

(124) ##STR00006##

(125) The title compound (96%, dark red-purple solid) was prepared from fluorescein ditriflate and piperidine-d.sub.11 according to the procedure described for Example 1. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.11-8.05 (m, 1H), 7.66 (td, J=7.4, 1.8 Hz, 1H), 7.62 (td, J=7.3, 1.7 Hz, 1H), 7.26-7.21 (m, 1H), 7.17 (d, J=9.4 Hz, 2H), 7.06 (dd, J=9.4, 2.6 Hz, 2H), 7.01 (d, J=2.5 Hz, 2H); Analytical HPLC: t.sub.R=12.7 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.30H.sub.11D.sub.20N.sub.2O.sub.3 [M+H].sup.+ 487.3585, found 487.3588.

Example 5. 2-(3,6-Bis(morpholino-d.SUB.8.)xanthylium-9-yl)benzoate

(126) ##STR00007##

(127) The title compound (88%, pink solid) was prepared from fluorescein ditriflate and morpholine-2,2,3,3,5,5,6,6-d.sub.8 according to the procedure described for Example 1. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.03-7.99 (m, 1H), 7.74 (td, J=7.4, 1.4 Hz, 1H), 7.69 (td, J=7.4, 1.2 Hz, 1H), 7.21-7.17 (m, 1H), 6.83 (d, J=2.4 Hz, 2H), 6.77 (dd, J=8.9, 2.5 Hz, 2H), 6.71 (d, J=8.9 Hz, 2H); Analytical HPLC: t.sub.R=10.2 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.28H.sub.11D.sub.16N.sub.2O.sub.5 [M+H].sup.+ 487.2919, found 487.2926.

Example 6. 2-(3,7-Bis(bis(methyl-d.SUB.3.)amino)-5,5-dimethyldibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(128) ##STR00008##

(129) The title compound (89%, off-white solid) was prepared from Si-fluorescein ditriflate (Grimm, J. B. et al. Nat. Methods 2015, 12, 244-250) and dimethyl-d.sub.6-amine hydrochloride according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.96 (dt, J=7.6, 1.0 Hz, 1H), 7.63 (td, J=7.5, 1.2 Hz, 1H), 7.53 (td, J=7.5, 1.0 Hz, 1H), 7.29 (dt, J=7.7, 0.9 Hz, 1H), 6.96 (d, J=2.9 Hz, 2H), 6.78 (d, J=8.9 Hz, 2H), 6.54 (dd, J=8.9, 2.9 Hz, 2H), 0.64 (s, 3H), 0.60 (s, 3H); Analytical HPLC: t.sub.R=10.4 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); MS (ESI) calcd for C.sub.26H.sub.17D.sub.12N.sub.2O.sub.2Si [M+H].sup.+ 441.3, found 441.2.

Example 7. 2-(3,7-Bis(azetidin-1-yl-d.SUB.6.)-5,5-dimethyldibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(130) ##STR00009##

(131) The title compound (57%, off-white solid) was prepared from Si-fluorescein ditriflate and azetidine-2,2,3,3,4,4-d.sub.6 hydrochloride according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.96 (dt, J=7.6, 1.0 Hz, 1H), 7.64 (td, J=7.5, 1.1 Hz, 1H), 7.54 (td, J=7.5, 0.8 Hz, 1H), 7.31 (dt, J=7.7, 0.9 Hz, 1H), 6.75 (d, J=8.7 Hz, 2H), 6.67 (d, J=2.6 Hz, 2H), 6.25 (dd, J=8.6, 2.7 Hz, 2H), 0.61 (s, 3H), 0.59 (s, 3H); Analytical HPLC: t.sub.R=12.5 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.28H.sub.17D.sub.12N.sub.2O.sub.2Si [M+H].sup.+ 465.2746, found 465.2749.

Example 8. 2-(5,5-Dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(132) ##STR00010##

(133) The title compound (96%, pale blue solid) was prepared from Si-fluorescein ditriflate and pyrrolidine according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.95 (dt, J=7.6, 0.9 Hz, 1H), 7.62 (td, J=7.5, 1.1 Hz, 1H), 7.52 (td, J=7.5, 0.9 Hz, 1H), 7.31-7.27 (m, 1H), 6.79 (d, J=2.7 Hz, 2H), 6.76 (d, J=8.8 Hz, 2H), 6.38 (dd, J=8.8, 2.8 Hz, 2H), 3.34-3.24 (m, 8H), 2.05-1.93 (m, 8H), 0.63 (s, 3H), 0.60 (s, 3H); .sup.13C NMR (CDCl.sub.3, 101 MHz) δ 171.0 (C), 154.9 (C), 146.8 (C), 137.2 (C), 133.7 (CH), 131.0 (C), 128.6 (CH), 128.4 (CH), 127.2 (C), 125.6 (CH), 124.7 (CH), 115.9 (CH), 112.7 (CH), 92.5 (C), 47.6 (CH.sub.2), 25.6 (CH.sub.2), 0.6 (CH.sub.3), −1.3 (CH.sub.3); Analytical HPLC: >99% purity (5 μL injection; 30-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.30H.sub.33N.sub.2O.sub.2Si [M+H].sup.+ 481.2306, found 481.2317.

Example 9. 2-(5,5-Dimethyl-3,7-bis(pyrrolidin-1-yl-d.SUB.8.)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(134) ##STR00011##

(135) The title compound (90%, off-white solid) was prepared from Si-fluorescein ditriflate and pyrrolidine-2,2,3,3,4,4,5,5-d.sub.8 according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.95 (dt, J=7.7, 1.0 Hz, 1H), 7.61 (td, J=7.5, 1.1 Hz, 1H), 7.52 (td, J=7.5, 0.9 Hz, 1H), 7.28 (dt, J=7.8, 1.0 Hz, 1H), 6.79 (d, J=2.8 Hz, 2H), 6.76 (d, J=8.8 Hz, 2H), 6.38 (dd, J=8.8, 2.8 Hz, 2H), 0.63 (s, 3H), 0.59 (s, 3H); Analytical HPLC: t.sub.R=13.0 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.30H.sub.17D.sub.16N.sub.2O.sub.2Si [M+H].sup.+ 497.3310, found 497.3312.

Example 10. 2-(3,6-Bis(bis(methyl-d.SUB.3.)amino)-10,10-dimethylanthracen-9-ylium-9(10H)-yl)benzoate

(136) ##STR00012##

(137) The title compound (90%, pale blue solid) was prepared from carbofluorescein ditriflate (Grimm, J. B. et al. ACS Chem. Biol. 2013, 8, 1303-1310) and dimethyl-d.sub.6-amine hydrochloride according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.01-7.96 (m, 1H), 7.58 (td, J=7.4, 1.5 Hz, 1H), 7.53 (td, J=7.4, 1.3 Hz, 1H), 7.09-7.04 (m, 1H), 6.88 (d, J=2.7 Hz, 2H), 6.60 (d, J=8.8 Hz, 2H), 6.50 (dd, J=8.8, 2.6 Hz, 2H), 1.88 (s, 3H), 1.77 (s, 3H); Analytical HPLC: t.sub.R=10.3 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 600 nm); MS (ESI) calcd for C.sub.27H.sub.17D12N.sub.2O.sub.2 [M+H].sup.+ 425.3, found 425.2.

Example 11. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)-10,10-dimethylanthracen-9-ylium-9(10H)-yl)benzoate

(138) ##STR00013##

(139) A vial was charged with carbofluorescein ditriflate (150 mg, 0.241 mmol), azetidine-2,2,3,3,4,4-d.sub.6 hydrochloride (120 mg, 1.20 mmol, 5 eq), RuPhos-G3-palladacycle (20.2 mg, 24.1 μmol, 0.1 eq), RuPhos (11.2 mg, 24.1 μmol, 0.1 eq), and Cs.sub.2CO.sub.3 (628 mg, 1.93 mmol, 8 eq). The vial was sealed and evacuated/backfilled with nitrogen (3×). Dioxane (2 mL) was added, and the reaction was flushed again with nitrogen (3×). The reaction was then stirred at 100° C. for 4 h. It was subsequently cooled to room temperature, filtered through Celite with CH.sub.2Cl.sub.2, and concentrated to dryness. Purification by silica gel chromatography (10-100% EtOAc/hexanes, linear gradient afforded the title compound (59 mg, 55%) as a pale blue solid. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.00-7.95 (m, 1H), 7.58 (td, J=7.4, 1.4 Hz, 1H), 7.53 (td, J=7.4, 1.2 Hz, 1H), 7.08-7.04 (m, 1H), 6.58 (d, J=2.4 Hz, 2H), 6.55 (d, J=8.6 Hz, 2H), 6.20 (dd, J=8.6, 2.4 Hz, 2H), 1.82 (s, 3H), 1.72 (s, 3H); Analytical HPLC: t.sub.R=11.8 min, 98.5% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 600 nm); HRMS (ESI) calcd for C.sub.29H.sub.17D.sub.12N.sub.2O.sub.2[M+H].sup.+ 449.2977, found 449.2980.

Example 12. 2-(10,10-Dimethyl-3,6-bis(pyrrolidin-1-yl-d.SUB.8.)anthracen-9-ylium-9(10H)-yl)benzoate

(140) ##STR00014##

(141) The title compound (91%, pale blue solid) was prepared from carbofluorescein ditriflate and pyrrolidine-2,2,3,3,4,4,5,5-d.sub.8 according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.00-7.96 (m, 1H), 7.57 (td, J=7.3, 1.5 Hz, 1H), 7.52 (td, J=7.4, 1.3 Hz, 1H), 7.08-7.04 (m, 1H), 6.72 (d, J=2.5 Hz, 2H), 6.58 (d, J=8.7 Hz, 2H), 6.35 (dd, J=8.7, 2.5 Hz, 2H), 1.88 (s, 3H), 1.77 (s, 3H); Analytical HPLC: t.sub.R=9.8 min, >99% purity (5 μL injection; 30-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 600 nm); HRMS (ESI) calcd for C.sub.31H.sub.17D.sub.16N.sub.2O.sub.2 [M+H].sup.+ 481.3541, found 481.3545.

Example 13. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)xanthylium-9-yl)-4-(tert-butoxycarbonyl)benzoate

(142) ##STR00015##

(143) The title compound (72%, dark red-purple solid) was prepared from 6-tert-butoxycarbonylfluorescein ditriflate (Grimm, J. B. et al. Nat. Methods 2015, 12, 244-250) and azetidine-2,2,3,3,4,4-d.sub.6 hydrochloride according to procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.19 (dd, J=8.0, 1.3 Hz, 1H), 8.01 (dd, J=8.0, 0.5 Hz, 1H), 7.77-7.70 (m, 1H), 6.53 (d, J=8.6 Hz, 2H), 6.21 (d, J=2.3 Hz, 2H), 6.09 (dd, J=8.6, 2.3 Hz, 2H), 1.54 (s, 9H); Analytical HPLC: t.sub.R=12.9 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.3DH.sub.19D.sub.12N.sub.2O.sub.5 [M+H].sup.+ 523.2981, found 523.2982.

Example 14. 4-(tert-Butoxycarbonyl)-2-(3,6-di(pyrrolidin-1-yl)xanthylium-9-yl)benzoate

(144) ##STR00016##

(145) The title compound (59%, dark red-purple solid) was prepared from 6-tert-butoxycarbonylfluorescein ditriflate and pyrrolidine according to the procedure described for Example 1. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.20 (dd, J=8.1, 1.7 Hz, 1H), 8.11 (d, J=8.1 Hz, 1H), 7.78 (d, J=1.7 Hz, 1H), 7.22 (d, J=9.4 Hz, 2H), 6.87 (dd, J=9.4, 2.3 Hz, 2H), 6.77 (d, J=2.3 Hz, 2H), 3.65-3.52 (m, 8H), 2.20-2.06 (m, 8H), 1.59 (s, 9H); .sup.13C NMR (CD.sub.3OD, 101 MHz) δ 172.4 (C), 166.1 (C), 162.2 (C), 158.9 (C), 156.1 (C), 145.9 (C), 133.8 (C), 133.6 (C), 132.7 (CH), 131.5 (CH), 131.2 (CH), 131.0 (CH), 115.9 (CH), 115.1 (C), 97.6 (CH), 83.1 (C), 49.9 (CH.sub.2), 28.4 (CH.sub.3), 26.2 (CH.sub.2); Analytical HPLC: t.sub.R=13.7 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.33H.sub.35N.sub.2O.sub.5 [M+H].sup.+ 539.2540, found 539.2544.

Example 15. 2-(3,6-Bis(pyrrolidin-1-yl-d.SUB.8.)xanthylium-9-yl)-4-(tert-butoxycarbonyl)benzoate

(146) ##STR00017##

(147) The title compound (58%, dark red-purple solid) was prepared from 6-tert-butoxycarbonylfluorescein ditriflate and pyrrolidine-2,2,3,3,4,4,5,5-d.sub.8 according to the procedure described for Example 1. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.20 (dd, J=8.2, 1.7 Hz, 1H), 8.11 (d, J=8.1 Hz, 1H), 7.78 (d, J=1.6 Hz, 1H), 7.21 (d, J=9.3 Hz, 2H), 6.87 (dd, J=9.3, 2.4 Hz, 2H), 6.76 (d, J=2.3 Hz, 2H), 1.59 (s, 9H); Analytical HPLC: t.sub.R=13.6 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.33H.sub.19D.sub.16N.sub.2O.sub.5 [M+H].sup.+ 555.3545, found 555.3544.

Example 16. 2-(3,7-Bis(azetidin-1-yl-d.SUB.6.)-5,5-dimethyldibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-(tert-butoxycarbonyl)benzoate

(148) ##STR00018##

(149) The title compound (48%, off-white foam) was prepared from 6-tert-butoxycarbonyl-Si-fluorescein ditriflate (Grimm, J. B. et al. Nat. Methods 2015, 12, 244-250) and azetidine-2,2,3,3,4,4-d.sub.6 hydrochloride according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.11 (dd, J=8.0, 1.3 Hz, 1H), 7.95 (dd, J=8.0, 0.6 Hz, 1H), 7.83-7.80 (m, 1H), 6.82 (d, J=8.7 Hz, 2H), 6.66 (d, J=2.7 Hz, 2H), 6.29 (dd, J=8.7, 2.7 Hz, 2H), 1.54 (s, 9H), 0.64 (s, 3H), 0.58 (s, 3H); Analytical HPLC: t.sub.R=14.1 min, 98.8% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.33H.sub.25D.sub.12N.sub.2O.sub.4Si [M+H].sup.+ 565.3270, found 565.3277.

Example 17. 4-(tert-Butoxycarbonyl)-2-(5,5-dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(150) ##STR00019##

(151) The title compound (81%, off-white solid) was prepared from 6-tert-butoxycarbonyl-Si-fluorescein ditriflate and pyrrolidine according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.10 (dd, J=8.0, 1.3 Hz, 1H), 7.96 (dd, J=8.0, 0.5 Hz, 1H), 7.83-7.79 (m, 1H), 6.84 (d, J=8.8 Hz, 2H), 6.79 (d, J=2.7 Hz, 2H), 6.44 (dd, J=8.8, 2.8 Hz, 2H), 3.35-3.25 (m, 8H), 2.04-1.95 (m, 8H), 1.53 (s, 9H), 0.67 (s, 3H), 0.60 (s, 3H); .sup.13C NMR (CDCl.sub.3, 101 MHz) δ 170.6 (C), 164.5 (C), 155.9 (C), 146.8 (C), 137.2 (C), 136.3 (C), 130.5 (C), 129.8 (CH), 129.3 (C), 128.1 (CH), 125.5 (CH), 125.1 (CH), 115.9 (CH), 113.2 (CH), 92.2 (C), 82.2 (C), 47.6 (CH.sub.2), 28.2 (CH.sub.3), 25.6 (CH.sub.2), 0.2 (CH.sub.3), −0.5 (CH.sub.3); Analytical HPLC: t.sub.R=14.6 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.35H.sub.41N.sub.2O.sub.4Si [M+H].sup.+ 581.2830, found 581.2839.

Example 18. 4-(tert-Butoxycarbonyl)-2-(5,5-dimethyl-3,7-bis(pyrrolidin-1-yl-d.SUB.8.)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(152) ##STR00020##

(153) The title compound (87%, off-white solid) was prepared from 6-tert-butoxycarbonyl-Si-fluorescein ditriflate and pyrrolidine-2,2,3,3,4,4,5,5-d.sub.8 according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.10 (dd, J=8.0, 1.3 Hz, 1H), 7.95 (dd, J=8.0, 0.6 Hz, 1H), 7.83-7.78 (m, 1H), 6.84 (d, J=8.8 Hz, 2H), 6.79 (d, J=2.7 Hz, 2H), 6.43 (dd, J=8.8, 2.8 Hz, 2H), 1.53 (s, 9H), 0.67 (s, 3H), 0.59 (s, 3H); Analytical HPLC: t.sub.R=14.4 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.35H.sub.25D.sub.16N.sub.2O.sub.4Si [M+H].sup.+ 597.3834, found 597.3835.

Example 19. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)-10,10-dimethylanthracen-9-ylium-9(10H)-yl)-4-(tert-butoxycarbonyl)benzoate

(154) ##STR00021##

(155) The title compound (42%, blue solid) was prepared from 6-tert-butoxycarbonyl-carbofluorescein ditriflate (Grimm, J. B. et al. Nat. Methods 2017, 14, 987-994) and azetidine-2,2,3,3,4,4-d.sub.6 hydrochloride according to the procedure described for Example 11. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.17 (dd, J=8.1, 1.5 Hz, 1H), 8.04 (d, J=8.1 Hz, 1H), 7.53 (d, J=1.3 Hz, 1H), 6.72 (d, J=2.3 Hz, 2H), 6.67 (d, J=8.7 Hz, 2H), 6.32 (dd, J=8.6, 2.3 Hz, 2H), 1.82 (s, 3H), 1.72 (s, 3H), 1.55 (s, 9H); HRMS (ESI) calcd for C.sub.34H.sub.25D.sub.12N.sub.2O.sub.4 [M+H].sup.+ 549.3501, found 549.3503.

Example 20. 4-(tert-Butoxycarbonyl)-2-(10,10-dimethyl-3,6-di(pyrrolidin-1-yl)anthracen-9-ylium-9(10H)-yl)benzoate

(156) ##STR00022##

(157) The title compound (79%, blue solid) was prepared from 6-tert-butoxycarbonyl-carbofluorescein ditriflate and pyrrolidine according to the procedure described for Example 1. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.15 (dd, J=8.1, 1.7 Hz, 1H), 8.06 (d, J=8.1 Hz, 1H), 7.63 (d, J=1.6 Hz, 1H), 7.01 (d, J=2.7 Hz, 2H), 6.99 (d, J=9.4 Hz, 2H), 6.60 (dd, J=9.2, 2.4 Hz, 2H), 3.63-3.52 (m, 8H), 2.16-2.06 (m, 8H), 1.85 (s, 3H), 1.77 (s, 3H), 1.58 (s, 9H); Analytical HPLC: t.sub.R=14.2 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 600 nm); HRMS (ESI) calcd for C.sub.36H.sub.41N.sub.2O.sub.4 [M+H].sup.+ 565.3061, found 565.3071.

Example 21. 4-(tert-Butoxycarbonyl)-2-(10,10-dimethyl-3,6-bis(pyrrolidin-1-yl-d.SUB.8.)anthracen-9-ylium-9(10H)-yl)benzoate

(158) ##STR00023##

(159) The title compound (50%, blue solid) was prepared from 6-tert-butoxycarbonyl-carbofluorescein ditriflate and pyrrolidine-2,2,3,3,4,4,5,5-d.sub.8 according to the procedure described for Example 1. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.15 (dd, J=8.1, 1.6 Hz, 1H), 8.06 (d, J=8.0 Hz, 1H), 7.64 (d, J=1.5 Hz, 1H), 7.01 (d, J=2.7 Hz, 2H), 7.00 (d, J=9.0 Hz, 2H), 6.60 (dd, J=9.2, 2.4 Hz, 2H), 1.85 (s, 3H), 1.77 (s, 3H), 1.58 (s, 9H); HRMS (ESI) calcd for C.sub.36H.sub.25D.sub.16N.sub.2O.sub.4 [M+H].sup.+ 581.4065, found 581.4064.

Example 22. 2-(3,6-Di(azetidin-1-yl)xanthylium-9-yl-1,2,4,5,7,8-d.SUB.6.)benzoate

(160) ##STR00024##

(161) Step 1: Phthalic anhydride (765 mg, 5.16 mol) and 1,3-dihydroxybenzene-d.sub.6 (1.20 g, 10.3 mmol, 2 eq) were combined in methanesulfonic acid-d.sub.4 (5 mL) and stirred at 85° C. for 48 h. The dark brown reaction mixture was cooled to room temperature, poured into D.sub.2O (40 mL), and vigorously stirred for 18 h. The resulting suspension was filtered; the filter cake was washed with D.sub.2O and thoroughly dried to provide 1.84 g crude fluorescein-1′,2′,4′,5′,7′,8′-d.sub.6 as a brown solid. This material was suspended in CH.sub.2Cl.sub.2 (25 mL) and cooled to 0° C. Pyridine (3.50 mL, 43.2 mmol, 8 eq) and trifluoromethanesulfonic anhydride (3.64 mL, 21.6 mmol, 4 eq) were added, and the ice bath was removed. The reaction was stirred at room temperature for 18 h. It was subsequently diluted with water and extracted with CH.sub.2Cl.sub.2 (2×). The combined organic extracts were washed with saturated CuSO.sub.4 and brine, dried over anhydrous MgSO.sub.4, filtered, and concentrated in vacuo. Silica gel chromatography (0-30% EtOAc/hexanes, linear gradient) yielded 1.69 g (54%, 2 steps) of fluorescein-1′,2′,4′,5′,7′,8′-d.sub.6 ditriflate as a colorless foam. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.10-8.06 (m, 1H), 7.74 (td, J=7.4, 1.4 Hz, 1H), 7.70 (td, J=7.4, 1.2 Hz, 1H), 7.21-7.16 (m, 1H); .sup.19F NMR (CDCl.sub.3, 376 MHz) δ 73.13 (s); Analytical HPLC: t.sub.R=15.3 min, >99% purity (5 μL injection; 30-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 254 nm); HRMS (ESI) calcd for C.sub.22H.sub.5D.sub.6F.sub.6O.sub.9S.sub.2[M+H].sup.+ 603.0120, found 603.0127.

(162) Step 2: The title compound (49%, purple solid) was prepared from fluorescein-1′,2′,4′,5′,7′,8′-d.sub.6 ditriflate (Step 1) and azetidine according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.02-7.95 (m, 1H), 7.63 (td, J=7.5, 1.3 Hz, 1H), 7.57 (td, J=7.4, 1.1 Hz, 1H), 7.19-7.15 (m, 1H), 3.90 (t, J=7.3 Hz, 8H), 2.37 (p, J=7.3 Hz, 4H); Analytical HPLC: t.sub.R=10.8 min, 97.5% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.26H.sub.17D.sub.6N.sub.2O.sub.3 [M+H].sup.+ 417.2080, found 417.2081.

Example 23. 2-(3,6-Di(azetidin-1-yl)xanthylium-9-yl-1,2,4,5,7,8-d.SUB.6.)benzoate-d.SUB.4

(163) ##STR00025##

(164) Step 1: The procedure described for Example 22, Step 1 was used to prepare fluorescein-1′,2′,4,4′,5,5′,6,7,7′,8′-d.sub.10 ditriflate (50%, white foam) from 1,3-dihydroxybenzene-d.sub.6 and phthalic anhydride-d.sub.4. .sup.19F NMR (CDCl.sub.3, 376 MHz) δ 73.13 (s); Analytical HPLC: t.sub.R=15.3 min, >99% purity (5 μL injection; 30-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 254 nm); HRMS (ESI) calcd for C.sub.22HD.sub.10F.sub.6O.sub.9S.sub.2 [M+H].sup.+ 607.0371, found 607.0373.

(165) Step 2: The title compound (74%, purple solid) was prepared from fluorescein-1′,2′,4,4′,5,5′,6,7,7′,8′-d.sub.10 ditriflate (Step 1) and azetidine according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 3.90 (t, J=7.3 Hz, 8H), 2.37 (p, J=7.3 Hz, 4H); Analytical HPLC: t.sub.R=10.9 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.26H.sub.13D.sub.10N.sub.2O.sub.3 [M+H].sup.+ 421.2331, found 421.2322.

Example 24. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)xanthylium-9-yl-1,2,4,5,7,8-d.SUB.6.)benzoate

(166) ##STR00026##

(167) The title compound (90%, purple solid) was prepared from fluorescein-1′,2′,4′,5′,7′,8′-d.sub.6 ditriflate (Example 22, Step 1) and azetidine-2,2,3,3,4,4-d.sub.6 hydrochloride according to the procedure described for Example 1. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.01-7.96 (m, 1H), 7.63 (td, J=7.4, 1.3 Hz, 1H), 7.57 (td, J=7.4, 1.1 Hz, 1H), 7.19-7.14 (m, 1H); Analytical HPLC: t.sub.R=10.9 min, 98.4% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.26H.sub.5D.sub.18N.sub.2O.sub.3 [M+H].sup.+ 429.2833, found 429.2834.

Example 25. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)xanthylium-9-yl-1,2,4,5,7,8-d.SUB.6.)benzoate-d.SUB.4

(168) ##STR00027##

(169) The title compound (64%, purple solid) was prepared from fluorescein-1′,2′,4,4′,5,5′,6,7,7′,8′-d.sub.10 ditriflate (Example 23, Step 1) and azetidine-2,2,3,3,4,4-d.sub.6 hydrochloride according to the procedure described for Example 1. Analytical HPLC: t.sub.R=10.8 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.26HD.sub.22N.sub.2O.sub.3 [M+H].sup.+ 433.3084, found 433.3083.

Example 26. 7-(Azetidin-1-yl-d.SUB.6.)-4-methyl-2H-chromen-2-one

(170) ##STR00028##

(171) A vial was charged with 4-methylumbelliferone triflate (Kövér, J.; Antus, S. Z. Naturforsch., B: J Chem. Sci. 2005, 60, 792-796; 175 mg, 0.568 mmol), azetidine-2,2,3,3,4,4-d.sub.6 hydrochloride (141 mg, 1.42 mmol, 2.5 eq), RuPhos-G3-palladacycle (23.7 mg, 28.4 μmol, 0.05 eq), RuPhos (13.2 mg, 28.4 μmol, 0.05 eq), and K.sub.2CO.sub.3 (314 mg, 2.27 mmol, 4 eq). The vial was sealed and evacuated/backfilled with nitrogen (3×). Dioxane (3 mL) was added, and the reaction was flushed again with nitrogen (3×). The reaction was then stirred at 100° C. for 18 h. It was subsequently cooled to room temperature, deposited onto Celite, and concentrated to dryness. Purification by silica gel chromatography (0-30% EtOAc/hexanes, linear gradient; dry load with Celite) afforded 20 mg (16%) of the title compound as a yellow solid. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.38 (d, J=8.6 Hz, 1H), 6.30 (dd, J=8.6, 2.3 Hz, 1H), 6.22 (d, J=2.3 Hz, 1H), 5.97 (q, J=1.2 Hz, 1H), 2.34 (d, J=1.2 Hz, 3H); Analytical HPLC: t.sub.R=11.9 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 350 nm); HRMS (ESI) calcd for C.sub.13H.sub.8D.sub.6NO.sub.2 [M+H].sup.+ 222.1396, found 222.1394.

Example 27. 4-Methyl-7-(pyrrolidin-1-yl)-2H-chromen-2-one

(172) ##STR00029##

(173) The title compound (92%, yellow solid) was prepared from 4-methylumbelliferone triflate and pyrrolidine according to the procedure described for Example 26. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.39 (d, J=8.8 Hz, 1H), 6.48 (dd, J=8.8, 2.4 Hz, 1H), 6.38 (d, J=2.4 Hz, 1H), 5.94 (q, J=1.1 Hz, 1H), 3.40-3.30 (m, 4H), 2.34 (d, J=1.1 Hz, 3H), 2.10-1.99 (m, 4H); .sup.13C NMR (CDCl.sub.3, 101 MHz) δ 162.3 (C), 155.9 (C), 153.2 (C), 150.5 (C), 125.5 (CH), 109.4 (C), 109.1 (CH), 108.8 (CH), 98.0 (CH), 47.8 (CH.sub.2), 25.6 (CH.sub.2), 18.6 (CH.sub.3); Analytical HPLC: t.sub.R=13.2 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 375 nm); HRMS (ESI) calcd for C.sub.14H.sub.16NO.sub.2 [M+H].sup.+ 230.1176, found 230.1180.

Example 28. 4-Methyl-7-(pyrrolidin-1-yl-d.SUB.8.)-2H-chromen-2-one

(174) ##STR00030##

(175) The title compound (86%, yellow solid) was prepared from 4-methylumbelliferone triflate and pyrrolidine-2,2,3,3,4,4,5,5-d.sub.8 according to the procedure described for Example 26. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.38 (d, J=8.8 Hz, 1H), 6.48 (dd, J=8.8, 2.4 Hz, 11H), 6.38 (d, J=2.4 Hz, 1H), 5.94 (q, J=1.1 Hz, 1H), 2.34 (d, J=1.1 Hz, 3H); Analytical HPLC: t.sub.R=13.1 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 375 nm); HRMS (ESI) calcd for C.sub.14H.sub.8D.sub.8NO.sub.2 [M+H].sup.+ 238.1678, found 238.1682.

Example 29. 3,7-Di(pyrrolidin-1-yl)phenoxazin-5-ium trifluoroacetate

(176) ##STR00031##

(177) A vial was charged with 10-acetyl-10H-phenoxazine-3,7-diyl bis(trifluoromethanesulfonate) (Grimm, J. B. et al. Nat. Methods 2015, 12, 244-250; 250 mg, 0.480 mmol), Pd.sub.2dba.sub.3 (43.9 mg, 48.0 μmol, 0.1 eq), XPhos (68.6 mg, 0.144 mmol, 0.3 eq), and Cs.sub.2CO.sub.3 (437 mg, 1.34 mmol, 2.8 eq). The vial was sealed and evacuated/backfilled with nitrogen (3×). Dioxane (2.5 mL) was added, and the reaction was flushed again with nitrogen (3×). Following the addition of pyrrolidine (96.1 μL, 1.15 mmol, 2.4 eq), the reaction was stirred at 80° C. for 4 h. It was then cooled to room temperature, filtered through Celite with CH.sub.2Cl.sub.2, and concentrated in vacuo. Purification by silica gel chromatography (0-40% EtOAc/toluene, linear gradient) afforded the N-acetyl leuco-dye (112 mg, 64%) as an off-white solid. The intermediate leuco-dye (112 mg, 0.308 mmol) was taken up in a mixture of CH.sub.2Cl.sub.2 (9 mL) and water (1 mL). DDQ (105 mg, 0.462 mmol, 1.5 eq) was added, and the reaction was stirred at room temperature for 2 h. The crude reaction mixture was then deposited onto Celite and concentrated to dryness. Silica gel chromatography (0-20% MeOH/CH.sub.2Cl.sub.2, linear gradient, with constant 1% v/v AcOH additive; dry load with Celite) followed by reverse phase HPLC (10-50% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive) afforded 127 mg (95%) of the title compound as a dark blue solid. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 7.77 (d, J=9.5 Hz, 2H), 7.25 (dd, J=9.4, 2.5 Hz, 2H), 6.81 (d, J=2.5 Hz, 211), 3.82-3.66 (m, 8H), 2.22-2.13 (m, 8H); .sup.13C NMR (CD.sub.3OD, 101 MHz) δ 156.6 (C), 150.4 (C), 135.5 (C), 135.3 (CH), 119.4 (CH), 98.0 (CH), 50.9 (bs, CH.sub.2), 26.2 (bs, CH.sub.2); Analytical HPLC: t.sub.R=10.4 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.20H.sub.22N.sub.30 [M].sup.+ 320.1757, found 320.1763.

Example 30. 3,7-Bis(pyrrolidin-1-yl-d.SUB.8.)phenoxazin-5-ium trifluoroacetate

(178) ##STR00032##

(179) The title compound (88%, dark blue solid) was prepared from 10-acetyl-10H-phenoxazine-3,7-diyl bis(trifluoromethanesulfonate) and pyrrolidine-2,2,3,3,4,4,5,5-d.sub.8 according to the procedure described for Example 29. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 7.77 (d, J=9.4 Hz, 2H), 7.25 (dd, J=9.5, 2.5 Hz, 2H), 6.80 (d, J=2.5 Hz, 2H); Analytical HPLC: t.sub.R=10.3 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.20H.sub.6D.sub.16N.sub.3O [M].sup.+ 336.2762, found 336.2765.

Example 31. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)xanthylium-9-yl)-4-carboxybenzoate

(180) ##STR00033##

(181) 2-(3,6-Bis(azetidin-1-yl-d.sub.6)xanthylium-9-yl)-4-(tert-butoxycarbonyl)benzoate (Example 13; 102 mg, 0.195 mmol) was taken up in CH.sub.2Cl.sub.2 (2.5 mL), and trifluoroacetic acid (0.5 mL) was added. The reaction was stirred at room temperature for 6 h. Toluene (3 mL) was added; the reaction mixture was concentrated to dryness and then azeotroped with MeOH three times to provide the title compound as a red-brown solid (109 mg, 96%, TFA salt). Analytical HPLC and NMR indicated that the material was >95% pure and did not require further purification prior to amide coupling. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.40 (d, J=8.1 Hz, 1H), 8.37 (dd, J=8.2, 1.5 Hz, 1H), 7.95-7.93 (m, 1H), 7.06 (d, J=9.2 Hz, 2H), 6.60 (dd, J=9.2, 2.2 Hz, 2H), 6.54 (d, J=2.2 Hz, 2H); Analytical HPLC: t.sub.R=9.9 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.27H.sub.11D.sub.12N.sub.2O.sub.5 [M+H].sup.+ 467.2355, found 467.2354.

Example 32. 4-Carboxy-2-(3,6-di(pyrrolidin-1-yl)xanthylium-9-yl)benzoate

(182) ##STR00034##

(183) The title compound (99%, dark red-purple solid, TFA salt) was prepared from 4-(tert-butoxycarbonyl)-2-(3,6-di(pyrrolidin-1-yl)xanthylium-9-yl)benzoate (Example 14) according to the procedure described for Example 32. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.42 (d, J=8.2 Hz, 1H), 8.39 (dd, J=8.2, 1.5 Hz, 1H), 7.99-7.96 (m, 1H), 7.11 (d, J=9.4 Hz, 2H), 6.92 (dd, J=9.4, 2.3 Hz, 2H), 6.84 (d, J=2.3 Hz, 2H), 3.68-3.56 (m, 8H), 2.21-2.07 (m, 8H); .sup.13C NMR (CD.sub.3OD, 101 MHz) δ 167.7 (C), 167.4 (C), 160.0 (C), 158.9 (C), 156.2 (C), 136.1 (C), 135.9 (C), 135.5 (C), 132.8 (CH), 132.31 (CH), 132.28 (CH), 132.0 (CH), 116.4 (CH), 114.9 (C), 97.8 (CH), 50.0 (CH.sub.2), 26.2 (CH.sub.2); Analytical HPLC: t.sub.R=10.8 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.29H.sub.27N.sub.2O.sub.5 [M+H].sup.+ 483.1914, found 483.1919.

Example 33. 2-(3,6-Bis(pyrrolidin-1-yl-d.SUB.8.)xanthylium-9-yl)-4-carboxybenzoate

(184) ##STR00035##

(185) The title compound (97%, dark red-purple solid, TFA salt) was prepared from 2-(3,6-bis(pyrrolidin-1-yl-d.sub.8)xanthylium-9-yl)-4-(tert-butoxycarbonyl)benzoate (Example 15) according to the procedure described for Example 32. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.41 (d, J=8.2 Hz, 1H), 8.38 (dd, J=8.2, 1.5 Hz, 1H), 7.99-7.96 (m, 1H), 7.11 (d, J=9.4 Hz, 2H), 6.91 (dd, J=9.3, 2.3 Hz, 2H), 6.83 (d, J=2.3 Hz, 2H); Analytical HPLC: t.sub.R=10.8 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.29H.sub.11D.sub.16N.sub.2O.sub.5 [M+H].sup.+ 499.2919, found 499.2922.

Example 34. 2-(3,7-Bis(azetidin-1-yl-d.SUB.6.)-5,5-dimethyldibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-carboxybenzoate

(186) ##STR00036##

(187) The title compound (98%, green solid, TFA salt) was prepared from 2-(3,7-bis(azetidin-1-yl-d.sub.6)-5,5-dimethyldibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-(tert-butoxycarbonyl)benzoate (Example 16) according to the procedure described for Example 32. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.29-8.25 (m, 2H), 7.80 (t, J=1.0 Hz, 1H), 6.90 (d, J=2.6 Hz, 2H), 6.87 (d, J=9.2 Hz, 2H), 6.33 (dd, J=9.2, 2.6 Hz, 2H), 0.60 (s, 3H), 0.53 (s, 3H); Analytical HPLC: t.sub.R=10.9 min, 97.8% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.29H.sub.17D.sub.12N.sub.2O.sub.4Si [M+H].sup.+ 509.2644, found 509.2648.

Example 35. 4-Carboxy-2-(5,5-dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(188) ##STR00037##

(189) The title compound (99%, blue-green solid, TFA salt) was prepared from 4-(tert-butoxycarbonyl)-2-(5,5-dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate (Example 17) according to the procedure described for Example 32. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.34 (d, J=8.2 Hz, 1H), 8.29 (dd, J=8.2, 1.7 Hz, 1H), 7.82 (d, J=1.6 Hz, 1H), 7.20 (d, J=2.7 Hz, 2H), 6.94 (d, J=9.4 Hz, 2H), 6.61 (dd, J=9.5, 2.7 Hz, 2H), 3.73-3.58 (m, 8H), 2.16-2.05 (m, 8H), 0.64 (s, 3H), 0.57 (s, 3H); .sup.13C NMR (CD.sub.3OD, 101 MHz) δ 170.3 (C), 168.0 (C), 167.5 (C), 152.9 (C), 149.3 (C), 142.5 (C), 141.6 (CH), 136.1 (C), 135.2 (C), 132.5 (CH), 132.4 (CH), 131.0 (CH), 129.1 (C), 122.6 (CH), 115.7 (CH), 49.9 (CH.sub.2), 26.1 (CH.sub.2), −0.8 (CH.sub.3), −1.8 (CH.sub.3); Analytical HPLC: t.sub.R=11.5 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.31H.sub.33N.sub.2O.sub.4Si [M+H].sup.+ 525.2204, found 525.2214.

Example 36. 4-Carboxy-2-(5,5-dimethyl-3,7-bis(pyrrolidin-1-yl-d.SUB.8.)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(190) ##STR00038##

(191) The title compound (˜100%, blue-green solid, TFA salt) was prepared from 4-(tert-butoxycarbonyl)-2-(5,5-dimethyl-3,7-bis(pyrrolidin-1-yl-d.sub.8)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate (Example 18) according to the procedure described for Example 32. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.34 (d, J=8.1 Hz, 1H), 8.29 (dd, J=8.2, 1.6 Hz, 1H), 7.83-7.81 (m, 1H), 7.19 (d, J=2.7 Hz, 2H), 6.94 (d, J=9.5 Hz, 2H), 6.60 (dd, J=9.5, 2.7 Hz, 2H), 0.64 (s, 3H), 0.57 (s, 3H); Analytical HPLC: t.sub.R=11.5 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.31H.sub.17D.sub.16N.sub.2O.sub.4Si [M+H].sup.+ 541.3208, found 541.3213.

Example 37. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)-10,10-dimethylanthracen-9-ylium-9(10H)-yl)-4-carboxybenzoate

(192) ##STR00039##

(193) The title compound (˜100%, blue-green solid) was prepared from 2-(3,6-bis(azetidin-1-yl-d.sub.6)-10,10-dimethylanthracen-9-ylium-9(10H)-yl)-4-(tert-butoxycarbonyl)benzoate (Example 19) according to the procedure described for Example 32. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.34 (d, J=8.2 Hz, 1H), 8.33-8.28 (m, 1H), 7.86-7.82 (m, 11H), 6.93 (d, J=9.1 Hz, 2H), 6.82 (d, J=2.3 Hz, 2H), 6.38 (dd, J=9.1, 2.2 Hz, 2H), 1.82 (s, 3H), 1.70 (s, 3H); MS (ESI) calcd for C.sub.30H.sub.17D.sub.12N.sub.2O.sub.4 [M+H].sup.+ 493.3, found 493.2.

Example 38. 4-Carboxy-2-(10,10-dimethyl-3,6-di(pyrrolidin-1-yl)anthracen-9-ylium-9(10H)-yl)benzoate

(194) ##STR00040##

(195) The title compound (˜100%, blue solid) was prepared from 4-(tert-butoxycarbonyl)-2-(10,10-dimethyl-3,6-di(pyrrolidin-1-yl)anthracen-9-ylium-9(10H)-yl)benzoate (Example 20) according to the procedure described for Example 32. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.38-8.28 (m, 2H), 7.92-7.83 (m, 1H), 7.10 (d, J=2.3 Hz, 2H), 6.99 (d, J=9.3 Hz, 2H), 6.67 (dd, J=9.2, 2.3 Hz, 2H), 3.71-3.59 (m, 8H), 2.17-2.07 (m, 8H), 1.88 (s, 3H), 1.76 (s, 3H); HRMS (ESI) calcd for C.sub.32H.sub.33N.sub.2O.sub.4 [M+H].sup.+ 509.2435, found 509.2435.

Example 39. 4-Carboxy-2-(10,10-dimethyl-3,6-bis(pyrrolidin-1-yl-d.SUB.8.)anthracen-9-ylium-9(10H)-yl)benzoate

(196) ##STR00041##

(197) The title compound (˜100%, blue solid) was prepared from 4-(tert-butoxycarbonyl)-2-(10,10-dimethyl-3,6-bis(pyrrolidin-1-yl-d.sub.8)anthracen-9-ylium-9(10H)-yl)benzoate (Example 21) according to the procedure described for Example 32. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.36 (dd, J=8.2, 0.4 Hz, 1H), 8.33 (dd, J=8.2, 1.6 Hz, 1H), 7.88-7.86 (m, 1H), 7.10 (d, J=2.4 Hz, 2H), 6.99 (d, J=9.3 Hz, 2H), 6.67 (dd, J=9.3, 2.4 Hz, 2H), 1.88 (s, 3H), 1.76 (s, 3H); MS (ESI) calcd for C.sub.32H.sub.17D.sub.16N.sub.2O.sub.4 [M+H].sup.+ 525.3, found 525.3.

Example 40. 2-(3,6-Bis(pyrrolidin-1-yl-d.SUB.8.)xanthylium-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate

(198) ##STR00042##

(199) 2-(3,6-Bis(pyrrolidin-1-yl-d.sub.8)xanthylium-9-yl)-4-carboxybenzoate (Example 34; 25 mg, 40.8 μmol, TFA salt) was combined with DSC (25.1 mg, 97.9 μmol, 2.4 eq) in DMF (1 mL). After adding Et.sub.3N (34.1 μL, 0.245 mmol, 6 eq) and DMAP (0.5 mg, 4.1 μmol, 0.1 eq), the reaction was stirred at room temperature for 1 h. Purification of the crude reaction mixture by reverse phase HPLC (10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive) afforded 20.8 mg (72%, TFA salt) of the title compound as a dark red-purple solid. .sup.1H NMR (DMSO-d.sub.6, 400 MHz) δ 8.49 (dd, J=8.3, 1.8 Hz, 1H), 8.43 (d, J=8.2 Hz, 1H), 8.15 (d, J=1.8 Hz, 1H), 7.11 (d, J=9.4 Hz, 2H), 6.91 (dd, J=9.3, 2.4 Hz, 2H), 6.83 (d, J=2.3 Hz, 2H), 2.91 (s, 4H); Analytical HPLC: t.sub.R=11.4 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.33H.sub.14D.sub.16N.sub.3O.sub.7 [M+H].sup.+ 596.3083, found 596.3089.

Example 41. 2-(5,5-Dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5h)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate

(200) ##STR00043##

(201) The title compound (87%, blue-green solid) was prepared from 4-carboxy-2-(5,5-dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5B)-yl)benzoate (Example 36) according to the procedure described for Example 41. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.27 (dd, J=8.0, 1.4 Hz, 1H), 8.07 (dd, J=8.0, 0.8 Hz, 1H), 8.00 (dd, J=1.3, 0.8 Hz, 1H), 6.79 (d, J=2.7 Hz, 2H), 6.74 (d, J=8.8 Hz, 2H), 6.43 (dd, J=8.9, 2.7 Hz, 2H), 3.35-3.25 (m, 8H), 2.88 (s, 4H), 2.04-1.94 (m, 8H), 0.64 (s, 3H), 0.58 (s, 3H); .sup.13C NMR (CDCl.sub.3, 101 MHz) δ 169.6 (C), 168.9 (C), 161.2 (C), 155.8 (C), 147.0 (C), 136.9 (C), 132.0 (C), 130.6 (CH), 130.1 (C), 129.7 (C), 128.3 (CH), 126.8 (CH), 126.2 (CH), 116.1 (CH), 113.2 (CH), 92.9 (C), 47.6 (CH.sub.2), 25.8 (CH.sub.2), 25.6 (CH.sub.2), 0.4 (CH.sub.3), −1.1 (CH.sub.3); Analytical HPLC: t.sub.R=12.2 min, 98.6% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.35H.sub.36N.sub.3O.sub.6Si [M+H].sup.+ 622.2368, found 622.2369.

Example 42. 2-(5,5-Dimethyl-3,7-bis(pyrrolidin-1-yl-d.SUB.8.)dibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate

(202) ##STR00044##

(203) The title compound (92%, blue-green solid) was prepared from 4-carboxy-2-(5,5-dimethyl-3,7-bis(pyrrolidin-1-yl-d.sub.8)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate (Example 37) according to the procedure described for Example 41. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.27 (dd, J=8.0, 1.4 Hz, 1H), 8.07 (dd, J=8.0, 0.6 Hz, 1H), 8.01 (dd, J=1.4, 0.8 Hz, 1H), 6.78 (d, J=2.8 Hz, 2H), 6.73 (d, J=8.8 Hz, 2H), 6.42 (dd, J=8.8, 2.8 Hz, 2H), 2.89 (s, 4H), 0.64 (s, 3H), 0.58 (s, 3H); Analytical HPLC: t.sub.R=12.1 min, 98.8% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.35H.sub.20D.sub.16N.sub.3O.sub.6Si [M+H].sup.+ 638.3372, found 638.3380.

Example 43. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)xanthylium-9-yl)-4-((2-(2-((6-chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)benzoate

(204) ##STR00045##

(205) 2-(3,6-Bis(azetidin-1-yl-d.sub.6)xanthylium-9-yl)-4-carboxybenzoate (Example 32; 25 mg, 43.1 μmol) was combined with DSC (26.5 mg, 0.103 mmol, 2.4 eq) in DMF (1 mL). After adding Et.sub.3N (36.0 μL, 0.258 mmol, 6 eq) and DMAP (0.5 mg, 4.3 μmol, 0.1 eq), the reaction was stirred at room temperature for 30 min. A solution of 2-(2-((6-chlorohexyl)oxy)ethoxy)-ethanamine (“HaloTag(O2)amine,” 43.6 mg, 0.129 mmol, 3 eq) in DMF (500 μL) was then added. The reaction was stirred an additional 2 h at room temperature. Purification of the crude reaction mixture by reverse phase HPLC (20-60% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive) afforded 22 mg (66%, TFA salt) of the title compound as a dark red solid. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.78 (t, J=5.5 Hz, 1H), 8.39 (d, J=8.2 Hz, 1H), 8.20 (dd, J=8.2, 1.8 Hz, 1H), 7.80 (d, J=1.7 Hz, 1H), 7.06 (d, J=9.2 Hz, 2H), 6.60 (dd, J=9.2, 2.2 Hz, 2H), 6.55 (d, J=2.2 Hz, 2H), 3.69-3.55 (m, 8H), 3.53 (t, J=6.6 Hz, 2H), 3.43 (t, J=6.5 Hz, 2H), 1.76-1.67 (m, 2H), 1.55-1.46 (m, 2H), 1.45-1.28 (m, 4H); Analytical HPLC: t.sub.R=12.3 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.37H.sub.31D.sub.12ClN.sub.3O.sub.6[M+H].sup.+ 672.3588, found 672.3590.

Example 44. 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(3,6-di(pyrrolidin-1-yl)xanthylium-9-yl)benzoate

(206) ##STR00046##

(207) The title compound (58%, dark red-purple solid, TFA salt) was prepared from 4-carboxy-2-(3,6-di(pyrrolidin-1-yl)xanthylium-9-yl)benzoate (Example 33) and 2-(2-((6-chlorohexyl)oxy)ethoxy)ethanamine according to the procedure described for Example 44. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.76 (t, J=5.3 Hz, 1H), 8.40 (d, J=8.2 Hz, 1H), 8.21 (dd, J=8.3, 1.8 Hz, 1H), 7.83 (d, J=1.7 Hz, 1H), 7.12 (d, J=9.3 Hz, 2H), 6.91 (dd, J=9.3, 2.3 Hz, 2H), 6.84 (d, J=2.3 Hz, 2H), 3.71-3.54 (m, 16H), 3.51 (d, J=6.6 Hz, 2H), 3.43 (t, J=6.5 Hz, 2H), 2.20-2.05 (m, 8H), 1.76-1.66 (m, 2H), 1.54-1.45 (m, 2H), 1.45-1.26 (m, 4H); Analytical HPLC: t.sub.R=13.2 min, 98.4% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.39H.sub.47ClN.sub.3O.sub.6[M+H].sup.+ 688.3148, found 688.3156.

Example 45. 2-(3,6-Bis(pyrrolidin-1-yl-d.SUB.8.)xanthylium-9-yl)-4-((2-(2-((6-chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)benzoate

(208) ##STR00047##

(209) The title compound (63%, dark red-purple solid, TFA salt) was prepared from 2-(3,6-bis(pyrrolidin-1-yl-d.sub.8)xanthylium-9-yl)-4-carboxybenzoate (Example 34) and 2-(2-((6-chlorohexyl)oxy)ethoxy)ethanamine according to the procedure described for Example 44. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.76 (t, J=5.2 Hz, 1H), 8.40 (d, J=8.2 Hz, 1H), 8.21 (dd, J=8.3, 1.8 Hz, 1H), 7.83 (d, J=1.7 Hz, 1H), 7.12 (d, J=9.3 Hz, 2H), 6.91 (dd, J=9.3, 2.3 Hz, 2H), 6.84 (d, J=2.3 Hz, 2H), 3.70-3.54 (m, 8H), 3.52 (t, J=6.6 Hz, 2H), 3.43 (t, J=6.5 Hz, 2H), 1.76-1.66 (m, 2H), 1.55-1.45 (m, 2H), 1.45-1.27 (m, 4H); Analytical HPLC: t.sub.R=13.1 min, 98.4% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.39H.sub.31D.sub.16ClN.sub.3O.sub.6[M+H].sup.+ 704.4152, found 704.4155.

Example 46. 2-(3,7-Bis(azetidin-1-yl-d.SUB.6.)-5,5-dimethyldibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-((2-(2-((6-chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)benzoate

(210) ##STR00048##

(211) The title compound (79%, pale blue-green solid) was prepared from 2-(3,7-bis(azetidin-1-yl-d.sub.6)-5,5-dimethyldibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-carboxybenzoate (Example 35) and 2-(2-((6-chlorohexyl)oxy)ethoxy)ethanamine according to the procedure described for Example 44. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.98 (d, J=7.9 Hz, 1H), 7.90 (dd, J=8.0, 1.4 Hz, 1H), 7.70-7.66 (m, 1H), 6.76 (s, 1H), 6.75 (d, J=8.7 Hz, 2H), 6.66 (d, J=2.7 Hz, 2H), 6.26 (dd, J=8.6, 2.7 Hz, 2H), 3.67-3.59 (m, 6H), 3.56-3.53 (m, 2H), 3.50 (t, J=6.6 Hz, 2H), 3.39 (t, J=6.7 Hz, 2H), 1.76-1.69 (m, 2H), 1.54-1.48 (m, 2H), 1.44-1.26 (m, 4H), 0.63 (s, 3H), 0.57 (s, 3H); Analytical HPLC: t.sub.R=13.2 min, 98.7% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.39H.sub.37D.sub.12ClN.sub.3O.sub.5Si [M+H].sup.+ 714.3878, found 714.3885.

Example 47. 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(5,5-dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(212) ##STR00049##

(213) 2-(5,5-Dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate (Example 42; 75 mg, 0.121 mmol) and 2-(2-((6-chlorohexyl)oxy)ethoxy)ethanamine (“HaloTag(O2)amine,” 61.1 mg, 0.181 mmol, 1.5 eq) were combined in DMF (3 mL), and DIEA (63.0 μL, 0.362 mmol, 3 eq) was added. After stirring the reaction at room temperature for 1 h, it was diluted with saturated NaHCO.sub.3 and extracted with EtOAc (2×). The combined organic extracts were washed with water and brine, dried over anhydrous MgSO.sub.4, filtered, and evaporated. Purification of the crude product by silica gel chromatography (10-100% EtOAc/toluene, linear gradient) provided the title compound as a pale blue-green solid (64 mg, 73%). .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.99 (d, J=7.9 Hz, 1H), 7.91 (dd, J=7.9, 1.4 Hz, 1H), 7.67-7.64 (m, 1H), 6.79 (d, J=2.7 Hz, 2H), 6.75 (s, 1H), 6.75 (d, J=8.8 Hz, 2H), 6.39 (dd, J=8.9, 2.7 Hz, 2H), 3.67-3.58 (m, 6H), 3.57-3.52 (m, 2H), 3.50 (t, J=6.7 Hz, 2H), 3.39 (t, J=6.7 Hz, 2H), 3.35-3.24 (m, 8H), 2.05-1.93 (m, 8H), 1.77-1.68 (m, 2H), 1.55-1.47 (m, 2H), 1.44-1.26 (m, 4H), 0.65 (s, 3H), 0.59 (s, 3H); Analytical HPLC: t.sub.R=13.5 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.41H.sub.53ClN.sub.3O.sub.5Si [M+H].sup.+ 730.3438, found 730.3442.

Example 48. 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(5,5-dimethyl-3,7-bis(pyrrolidin-1-yl-d.SUB.8.)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(214) ##STR00050##

(215) The title compound (77%, pale blue-green solid) was prepared from 2-(5,5-dimethyl-3,7-bis(pyrrolidin-1-yl-d.sub.8)dibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate (Example 43) and 2-(2-((6-chlorohexyl)oxy)ethoxy)ethanamine according to the procedure described for Example 48. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.99 (dd, J=8.0, 0.5 Hz, 1H), 7.91 (dd, J=8.0, 1.4 Hz, 1H), 7.67-7.63 (m, 1H), 6.78 (d, J=2.8 Hz, 2H), 6.747 (d, J=8.8 Hz, 2H), 6.744 (s, 1H), 6.39 (dd, J=8.8, 2.8 Hz, 2H), 3.66-3.58 (m, 6H), 3.57-3.52 (m, 2H), 3.50 (t, J=6.7 Hz, 2H), 3.39 (t, J=6.7 Hz, 2H), 1.77-1.68 (m, 2H), 1.55-1.47 (m, 2H), 1.44-1.35 (m, 2H), 1.34-1.25 (m, 2H), 0.65 (s, 3H), 0.58 (s, 3H); Analytical HPLC: t.sub.R=13.4 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.41H.sub.37D.sub.16ClN.sub.3O.sub.5Si [M+H].sup.+ 746.4442, found 746.4449.

Example 49. 2-(3,6-Bis(azetidin-1-yl-d.SUB.6.)-10,10-dimethylanthracen-9-ylium-9(10H)-yl)-4-((2-(2-((6-chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)benzoate

(216) ##STR00051##

(217) Step 1: The procedure described for Example 41 was used to prepare 2-(3,6-bis(azetidin-1-yl-d.sub.6)-10,10-dimethylanthracen-9-ylium-9(10H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate from 2-(3,6-bis(azetidin-1-yl-d.sub.6)-10,10-dimethylanthracen-9-ylium-9(10H)-yl)-4-carboxybenzoate (Example 38). MS (ESI) calcd for C.sub.34H.sub.20D.sub.12N.sub.3O.sub.6 [M+H].sup.+ 590.3, found 590.3.

(218) Step 2: The title compound (25%, blue solid) was prepared from 2-(3,6-bis(azetidin-1-yl-d.sub.6)-10,10-dimethylanthracen-9-ylium-9(10H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate (Step 1) and 2-(2-((6-chlorohexyl)oxy)ethoxy)ethanamine according to the procedure described for Example 48. .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.02 (d, J=7.9 Hz, 1H), 7.97-7.91 (m, 1H), 7.44-7.39 (m, 1H), 6.70 (s, 1H), 6.57 (d, J=2.4 Hz, 2H), 6.52 (d, J=8.6 Hz, 2H), 6.20 (dd, J=8.6, 2.4 Hz, 2H), 3.63-3.48 (m, 10H), 3.38 (t, J=6.6 Hz, 2H), 1.83 (s, 3H), 1.78-1.69 (m, 2H), 1.72 (s, 3H), 1.52-1.26 (m, 6H); Analytical HPLC: t.sub.R=12.6 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 600 nm); MS (ESI) calcd for C.sub.40H.sub.37D.sub.12ClN.sub.3O.sub.5 [M+H].sup.+ 698.4, found 698.3.

Example 50. 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(10,10-dimethyl-3,6-di(pyrrolidin-1-yl)anthracen-9-ylium-9(10H)-yl)benzoate

(219) ##STR00052##

(220) Step 1: The procedure described for Example 41 was used to prepare 2-(10,10-dimethyl-3,6-di(pyrrolidin-1-yl)anthracen-9-ylium-9(10H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate from 4-carboxy-2-(10,10-dimethyl-3,6-di(pyrrolidin-1-yl)anthracen-9-ylium-9(10H)-yl)benzoate (Example 39). MS (ESI) calcd for C.sub.36H.sub.36N.sub.3O.sub.6 [M+H].sup.+ 606.3, found 606.2.

(221) Step 2: The title compound (11%, blue solid) was prepared from 2-(10,10-dimethyl-3,6-di(pyrrolidin-1-yl)anthracen-9-ylium-9(10H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate (Step 1) and 2-(2-((6-chlorohexyl)oxy)ethoxy)ethanamine according to the procedure described for Example 48. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.77-8.72 (m, 1H), 8.35 (d, J=8.2 Hz, 1H), 8.16 (dd, J=8.3, 1.5 Hz, 1H), 7.77 (d, J=1.8 Hz, 1H), 7.10 (d, J=2.4 Hz, 2H), 7.00 (d, J=9.3 Hz, 2H), 6.67 (dd, J=9.2, 2.3 Hz, 2H), 3.72-3.54 (m, 16H), 3.51 (t, J=6.6 Hz, 2H), 3.43 (t, J=6.5 Hz, 2H), 2.20-2.05 (m, 8H), 1.87 (s, 3H), 1.77 (s, 3H), 1.74-1.66 (m, 2H), 1.54-1.47 (m, 2H), 1.42-1.31 (m, 4H); Analytical HPLC: t.sub.R=13.3 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 600 nm); MS (ESI) calcd for C.sub.42H.sub.53ClN.sub.3O.sub.5[M+H].sup.+ 714.4, found 714.3.

Example 51. 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(10,10-dimethyl-3,6-bis(pyrrolidin-1-yl-d.SUB.8.)anthracen-9-ylium-9(10H)-yl)benzoate

(222) ##STR00053##

(223) Step 1: The procedure described for Example 41 was used to prepare 2-(10,10-dimethyl-3,6-bis(pyrrolidin-1-yl-d.sub.8)anthracen-9-ylium-9(10H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate from 4-carboxy-2-(10,10-dimethyl-3,6-bis(pyrrolidin-1-yl-d.sub.8)anthracen-9-ylium-9(10H)-yl)benzoate (Example 40). MS (ESI) calcd for C.sub.36H.sub.20D.sub.16N.sub.3O.sub.6 [M+H].sup.+ 622.4, found 622.3.

(224) Step 2: The title compound (31%, blue solid) was prepared from 2-(10,10-dimethyl-3,6-bis(pyrrolidin-1-yl-d.sub.8)anthracen-9-ylium-9(10)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate (Step 1) and 2-(2-((6-chlorohexyl)oxy)ethoxy)ethanamine according to the procedure described for Example 48. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.75 (t, J=5.1 Hz, 1H), 8.35 (d, J=8.2 Hz, 1H), 8.16 (dd, J=8.2, 1.9 Hz, 1H), 7.77 (d, J=1.7 Hz, 1H), 7.10 (d, J=2.4 Hz, 2H), 7.00 (d, J=9.2 Hz, 2H), 6.66 (dd, J=9.3, 2.4 Hz, 2H), 3.70-3.54 (m, 8H), 3.51 (t, J=6.6 Hz, 2H), 3.43 (t, J=6.5 Hz, 2H), 1.86 (s, 3H), 1.76 (s, 3H), 1.75-1.67 (m, 2H), 1.50 (p, J=6.8 Hz, 2H), 1.43-1.31 (m, 4H); Analytical HPLC: t.sub.R=12.9 min, 98.4% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 600 nm); MS (ESI) calcd for C.sub.42H.sub.37D.sub.16ClN.sub.3O.sub.5[M+H].sup.+ 730.5, found 730.4.

Example 52. 4-((4-(((2-Amino-9H-purin-6-yl)oxy)methyl)benzyl)carbamoyl)-2-(3,6-di(pyrrolidin-1-yl)xanthylium-9-yl)benzoate

(225) ##STR00054##

(226) The title compound (66%, dark red-purple solid) was prepared from 4-carboxy-2-(3,6-di(pyrrolidin-1-yl)xanthylium-9-yl)benzoate (Example 33) and 6-((4-(aminomethyl)benzyl)oxy)-9H-purin-2-amine (“BG-NH.sub.2”) according to the procedure described for Example 44. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 9.30 (t, J=6.0 Hz, 1H), 8.40 (d, J=8.2 Hz, 1H), 8.26 (s, 1H), 8.22 (dd, J=8.3, 1.8 Hz, 1H), 7.84 (d, J=1.7 Hz, 1H), 7.51 (d, J=8.1 Hz, 2H), 7.41 (d, J=8.1 Hz, 2H), 7.10 (d, J=9.4 Hz, 2H), 6.89 (dd, J=9.3, 2.3 Hz, 2H), 6.83 (d, J=2.2 Hz, 2H), 5.62 (s, 2H), 4.60 (s, 2H), 3.66-3.57 (m, 8H), 2.20-2.08 (m, 8H); Analytical HPLC: t.sub.R=10.0 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.42H.sub.39N.sub.8O.sub.5 [M+H].sup.+ 735.3038, found 735.3046.

Example 53. 4-((4-(((2-Amino-9H-purin-6-yl)oxy)methyl)benzyl)carbamoyl)-2-(3,6-bis(pyrrolidin-1-yl-d)-9-xanthen-9-ylium-9-yl)benzoate

(227) ##STR00055##

(228) The title compound (68%, dark red-purple solid) was prepared from 2-(3,6-bis(pyrrolidin-1-yl-d.sub.8)xanthylium-9-yl)-4-carboxybenzoate (Example 34) and 6-((4-(aminomethyl)benzyl)oxy)-9H-purin-2-amine according to the procedure described for Example 44. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 9.30 (t, J=5.9 Hz, 1H), 8.40 (d, J=8.2 Hz, 1H), 8.216 (s, 1H), 8.215 (dd, J=8.2, 1.8 Hz, 1H), 7.84 (d, J=1.7 Hz, 1H), 7.50 (d, J=8.0 Hz, 2H), 7.40 (d, J=7.9 Hz, 2H), 7.09 (d, J=9.3 Hz, 2H), 6.88 (dd, J=9.3, 2.3 Hz, 2H), 6.82 (d, J=2.3 Hz, 2H), 5.60 (s, 2H), 4.60 (s, 2H); Analytical HPLC: t.sub.R=10.0 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 550 nm); HRMS (ESI) calcd for C.sub.42H.sub.23D.sub.16N.sub.8O.sub.5 [M+H].sup.+ 751.4042, found 751.4054.

Example 54. 4-((4-(((2-Amino-9H-purin-6-yl)oxy)methyl)benzyl)carbamoyl)-2-(5,5-dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(229) ##STR00056##

(230) The title compound (76%, blue solid) was prepared from 2-(5,5-dimethyl-3,7-di(pyrrolidin-1-yl)dibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate (Example 42) and 6-((4-(aminomethyl)benzyl)oxy)-9H-purin-2-amine according to the procedure described for Example 48. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.04 (dd, J=8.1, 1.4 Hz, 1H), 8.00 (dd, J=8.0, 0.7 Hz, 1H), 7.83 (s, 1H), 7.71-7.69 (m, 1H), 7.44 (d, J=8.2 Hz, 2H), 7.31 (d, J=8.2 Hz, 2H), 6.86 (d, J=2.8 Hz, 2H), 6.69 (d, J=8.9 Hz, 2H), 6.42 (dd, J=8.9, 2.8 Hz, 2H), 5.49 (s, 2H), 4.52 (s, 2H), 3.32-3.24 (m, 8H), 2.07-1.95 (m, 8H), 0.62 (s, 3H), 0.55 (s, 3H); Analytical HPLC: t.sub.R=10.5 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.44H.sub.45N.sub.8O.sub.4Si [M+H].sup.+ 777.3328, found 777.3339.

Example 55. 4-((4-(((2-Amino-9H-purin-6-yl)oxy)methyl)benzyl)carbamoyl)-2-(5,5-dimethyl-3,7-bis(pyrrolidin-1-yl-d.SUB.8.)dibenzo[b,e]silin-10-ylium-10(5H)-yl)benzoate

(231) ##STR00057##

(232) The title compound (78%, blue solid) was prepared from 2-(5,5-dimethyl-3,7-bis(pyrrolidin-1-yl-d.sub.8)dibenzo[b,e]silin-10-ylium-10(5H)-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate (Example 43) and 6-((4-(aminomethyl)benzyl)oxy)-9H-purin-2-amine according to the procedure described for Example 48. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ 8.02 (dd, J=8.0, 1.4 Hz, 1H), 7.98 (dd, J=8.0, 0.5 Hz, 1H), 7.81 (s, 1H), 7.69-7.67 (m, 1H), 7.42 (d, J=8.2 Hz, 2H), 7.29 (d, J=8.1 Hz, 2H), 6.84 (d, J=2.8 Hz, 2H), 6.67 (d, J=8.9 Hz, 2H), 6.40 (dd, J=8.9, 2.8 Hz, 2H), 5.47 (s, 2H), 4.50 (s, 2H), 0.60 (s, 3H), 0.53 (s, 3H); Analytical HPLC: t.sub.R=10.4 min, >99% purity (5 μL injection; 10-95% MeCN/H.sub.2O, linear gradient, with constant 0.1% v/v TFA additive; 20 min run; 1 mL/min flow; ESI; positive ion mode; detection at 650 nm); HRMS (ESI) calcd for C.sub.44H.sub.29D.sub.16N.sub.8O.sub.4Si [M+H].sup.+ 793.4332, found 793.4341.