NEW AGROCHEMICAL FORMULATIONS FOR FUSARICIDIN PRODUCING BACTERIA

Abstract

The present invention is directed to spraying liquid suitable for spraying plants, comprising spores of fusaricidin producing bacteria, at least one category (1) additive and/or at least one category (2) additive and less than 81 g per hectare of a category (3) additive, to solid and liquid formulations comprising spores of fusaricidin producing bacteria, at least one category (1) additive and/or at least one category (2) additive and optionally a category (3) additive, as well as to methods to control phytopathogenic fungi on plants applying such spraying liquids or formulations.

Claims

1. A spraying liquid suitable for spraying plants, comprising a. biomass comprising spores of fusaricidin producing bacteria, in an amount of 50 g per hectare to 2000 g per hectare, b. at least one category 1 additive or at least one category 2 additive or a mixture of at least one category 1 additive and at least one category 2 additive, in a total amount of 20 g per hectare to 2000 g per hectare, and c. a total amount of less than 81 g per hectare category 3 additives, wherein the at least one category 1 additive is selected from at least one group of additives 1a) to 1f): 1a) poloxamers, polyethylene glycol (A)—polypropylene glycol (B) block copolymers of the A-B-A or B-A-B type, comprising polyethylene glycol-polypropylene glycol-polyethylene glycol block polymers with a molar mass of approximately 950 to 6500 g/mol and a polyethylene oxide content of 10% to 50% w/w; 1b) polyvinylpyrolidines, polyvinylacetates, and statistical and block copolymers of polyvinyl pyrrolidone and polyvinyl acetate; 1c) polyoxyethylene sorbitol fatty acid esters (“polysorbates”), having a fatty acid chain with a length of 10 to 20 carbon atoms, from 10 to 150 oxyethylene units; 1d) fatty acid glycerol esters including fatty acid mono- and diesters of glycerol and polyglycerol, glycerolmonooleate, glyceroldioleate; 1e) alcohol alcoxylates; 1f) alkyl glucosides having an alkyl chain length C5-C15 and 1 to 10 glucoside units, and wherein the at least one category 2 additive is selected from at least one group of additives 2a) to 2c) 2a) polyethylene glycol-grafted polyacrylates, and salts thereof; 2b) ethoxylated polyethyleneimines; 2c) sodium lauryl ether sulfate, wherein the category 3 additive belongs to a group of additives 3a) to 3g) 3a) lignosulfonates; 3b) alkylbenzene sulfonates and salts thereof; 3c) alkylnaphthalene sulfonates and salts thereof; 3e) napthalene sulfonic acid co-condensates; 3f) alkyl sulfonates and salts thereof; 3g) fatty alcohol sulfates and salts thereof.

2. The spraying liquid according to claim 1, wherein the at least one category 1 additive of the groups 1a) to 1f) is selected from: for group 1a): poloxamers; for group 1 b) statistical copolymers of polyvinyl pyrrolidone and polyvinyl acetate; for group 1c) polyoxyethylene sorbitol oleic acid esters with 20 to 40 oxyethylene units; for group 1d) fatty acid mono- and diesters of glycerol and polyglycerol for group 1e) alcohol alcoxylates with 5 to 80 oxyethylene units; for group 1f) alkyl polyglucosides having an alkyl chain length C8-C10 and mono- and diglucosides with an overall polymerization degree of 1.5.

2. The spraying liquid according to claim 1, wherein the at least one category 1 additive of the groups 1a) to 1f) is selected from: for group 1a) from polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol, polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol, polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, or polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol; for group 1 b) polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol, or polyvinylpyrrolidone-polyvinylacetate statistical copolymer; for group 1c) polyoxyethylene (40) sorbitol hexaoleate, sorbitol ethoxylate esters, poly oxyethylene (20) sorbitan monolaurate, poly(oxyethylene) (20) sorbitan monooleate, sorbitan monooleate or sorbitol ethoxylate ester of polymerized fatty acid; for group 1d) mixtures of polyglycerolesters and fatty acid esters, or glycerolmon/diooleat; for group 1e) alkyl polyethylene glycol ether from C10-Guerbet alcohol, HLB 13, alkyl polyethylene glycol ether from C10-Guerbet alcohol, HLB 16, polyoxyethylene (23) lauryl ether or alkyl polyethylene glycol ether from C16 C18 fatty alcohol, HLB 18.5; for group 1f) alkyl polyglucoside.

3. A kit of at least two parts to prepare a spraying liquid according to claim 1, wherein the biomass comprising spores of fusaricidin producing bacteria are provided in a first concentrated form and at least one of the category 1 additive and/or category 2 additive and/or category 3 additive are provided in at least one further concentrated forms and wherein the relative amounts of the biomass comprising spores of fusaricidin producing bacteria and the relative amounts the category 1 additive and/or the category 2 additive and/or the category 3 additive in the kit are adapted to provide the amounts described in any one of the preceding claims.

4. The kit of parts according to claim 4, wherein the spraying liquid is prepared to be used in a method to control phytopathogenic fungi.

5. A method to control phytopathogenic fungi wherein a spraying liquid according to claim 1 is sprayed on plants.

6. The method to control phytopathogenic fungi according to claim 6, wherein the spraying liquid is sprayed in an amount of about 100 liter to about 1000 liter per hectare and the spraying liquid comprises biomass comprising spores of fusaricidin producing bacteria in an amount of 0.005% w/w to 2% w/w and the category 1 additive and/or the category 2 additive in a total amount of 0.005% w/w to 2% w/w.

7. The method to control phytopathogenic fungi according to claim 6, wherein the plants are selected from maize, small-grain cereals, cotton, canola, sunflowers, tobacco, peanuts, vegetables, grapevines, leafy vegetables, root & tuber vegetables, onions, strawberries, blueberries, strawberry, black currant, white currant, red currant, blackberry, raspberry, tomato, pepper, chilli, cucumber, aubergine, melons, watermelons, cucumbers, tomatoes, peppers & aubergines, herbs, turf and ornamentals.

8. The method to control phytopathogenic fungi according to claim 6, wherein the phytopathogenic fungi is Alternaria spp., Botrytis cinerea or Phytophthora infestans.

9. A solid or liquid formulation comprising: a. 5% w/w to 50% w/w biomass comprising spores of fusaricidin producing bacteria, b. 5% w/w to 50% w/w of at least one category 1 additive or at least one category 2 additive or a mixture of at least one category 1 additive or at least one category 2 additive, and c. less than 2% w/w of category 3 additives, wherein the at least one category 1 additive is selected from at least one group of additives 1a) to 1f): 1a) poloxamers, polyethylene glycol, polypropylene glycol or polyethylene glycol block polymers with a molar mass of approximately 950 to 6500 g/mol and a polyethylene oxide content of 10% to 50% w/w; 1b) polyvinylpyrolidines, polyvinylacetates and statistical and block copolymers of polyvinyl pyrrolidone and polyvinyl acetate, all with a molar mass of approximately 9000 to 70000 g/mol; 1c) polyoxyethylene sorbitol fatty acid esters (“polysorbates”), having a fatty acid chain with a length of 10 to 20 carbon atoms, from 10 to 150 oxyethylene units; 1d) fatty acid glycerol esters, fatty acid mono- and diesters of glycerol and polyglycerol, glycerolmonooleate, glyceroldioleate, polyglycerol fatty acid ester; 1e) alcohol alcoxylates, from 5 to 80 oxyethylene units; 1f) alkyl glucosides having an alkyl chain length C5-C15 and 1 to 10 glucoside units, and the at least one category 2 additive is selected from at least one group of additives 2a) to 2c) 2a) polyethylene glycol-grafted polyacrylates, and salts thereof; 2b) ethoxylated polyethyleneimines; 2c) sodium lauryl ether sulfate, and the category 3 additives are selected from the groups of additives 3a) to 3g) 3a) lignosulfonates; 3b) alkylbenzene sulfonates and salts thereof; 3c) alkylnaphthalene sulfonates and salts thereof; 3e) apthalene sulfonic acid co-condensates; 3f) alkyl sulfonates and salts thereof; 3g) fatty alcohol sulfates and salts thereof.

10. The solid or liquid formulation according to claim 10, comprising: a. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives, b. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives, c. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additives, d. at least one poly(oxyethylene) (20) sorbitan monooleate and at least one sorbitan monooleate as category 1 additives, or e. at least one polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol and at least one polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol as category 1 additives.

11. The solid formulation according to claim 10, comprising 2% w/w to 50% w/w of a category 1 additive, wherein the category 1 additive is a combination of a. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives, b. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives, c. at least one poly(oxyethylene) (20) sorbitan monooleate and at least one sorbitan monooleate, or d. at least one polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol and at least one polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol, as category 1 additives.

12. The liquid formulation according to claim 10, comprising: a. 2% w/w to 15% w/w of at least one category 1 additive selected from polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol, polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol and/or a mixture of polyglycerolesters and fatty acid esters, or a mixture of at least two of these, and b. 1% w/w to 15% w/w of at least one category 2 additive selected from methylmethacrylate graft copolymer.

13. A process for making a solid formulation comprising: a. 5.0% w/w to 50% w/w biomass comprising spores of fusaricidin producing bacteria, b. 5.0% w/w to 50% w/w of a category 1 additive, c. 0% w/w to 20% w/w of a category 2 additive, d. 0% w/w to 2% w/w of a category 3 additive, and e. 30% w/w to 50% w/w of auxiliaries. wherein the total amounts of a, b, c, d, and e are selected to result in a total amount of 100% w/w, comprising the following steps: A. providing biomass comprising spores of fusaricidin producing bacteria, B. dispersing said biomass in a continuous phase comprising, C. adding at least one category 1 additive, D. optionally adding at least one category 2 additive, E. optionally adding at least one category 3 additive, F. optionally adding auxiliaries, G. drying the biomass containing slurry by spray-drying, vacuum-drum drying, fluidized-bed drying or spray-granulation, Wherein steps C., D., E. and F. can be carried out at any time during the process.

14. A process for making a suspension concentrate (SC) formulation comprising: a. providing biomass comprising spores of fusaricidin producing bacteria, b. dispersing said biomass by shear or mechanical energy input in a continuous phase comprising, c. adding at least one category 1 additive, d. optionally adding at least one category 2 additive, e. optionally adding at least one category 3 additive, f. optionally adding auxiliaries, g. wherein steps c), d), e) and f) can be carried out at any time during the process. wherein the total amounts of a, b, c, d, and e are selected to result in a total amount of 100% w/w, comprising the following steps: H. providing biomass comprising spores of fusaricidin producing bacteria, I. dispersing said biomass in a continuous phase comprising, J. adding at least one category 1 additive, K. optionally adding at least one category 2 additive, L. optionally adding at least one category 3 additive, M. optionally adding auxiliaries, N. drying the biomass containing slurry by spray-drying, vacuum-drum drying, fluidized-bed drying or spray-granulation, Wherein steps C., D., E. and F. can be carried out at any time during the process.

15. A process for making a suspension concentrate (SC) formulation comprising: a. providing biomass comprising spores of fusaricidin producing bacteria, b. dispersing said biomass by shear or mechanical energy input in a continuous phase comprising, c. adding at least one category 1 additive, d. optionally adding at least one category 2 additive, e. optionally adding at least one category 3 additive, f. optionally adding auxiliaries, g. wherein steps c), d), e) and f) can be carried out at any time during the process.

Description

[0414] The embodiments listed below represent preferred exemplary embodiments of the invention but should not be understood to limit the invention. The amounts of category 1 additives, category 2 additives and, if present, category 3 additives, as well as the particular combinations of category 1 additives, category 2 additives and, if present, category 3 additives, disclosed in Examples 6 to 10 are especially preferred.

[0415] Embodiment 1: Spraying liquid suitable for spraying plants, comprising [0416] d. biomass comprising spores of fusaricidin producing bacteria, in an amount of 50 g per hectare to 2000 g per hectare, [0417] e. at least one category 1 additive or at least one category 2 additive or a mixture of at least one category 1 additive and at least one category 2 additive, in a total amount of 20 g per hectare to 2000 g per hectare, and [0418] f. a total amount of less than 81 g per hectare category 3 additives,
wherein the at least one category 1 additive is selected from at least one group of additives 1a) to 1f): [0419] 1a) poloxamers, polyethylene glycol (A)—polypropylene glycol (B) block copolymers of the A-B-A or B-A-B type, comprising polyethylene glycol-polypropylene glycol-polyethylene glycol block polymers with a molar mass of approximately 950 to 6500 g/mol and a polyethylene oxide content of 10% to 50% w/w; [0420] 1b) polyvinylpyrolidines, polyvinylacetates, and statistical and block copolymers of polyvinyl pyrrolidone and polyvinyl acetate; [0421] 1c) polyoxyethylene sorbitol fatty acid esters (“polysorbates”), having a fatty acid chain with a length of 10 to 20 carbon atoms, preferably 12 to 18 carbon atoms, most preferably dodecanoic acid and oleic acid and linoleic acid, from 10 to 150 oxyethylene units, preferably 20 to 80 oxyethylene units, most preferred 20 to 40 oxyethylene units; [0422] 1d) fatty acid glycerol esters including fatty acid mono- and diesters of glycerol and polyglycerol, glycerolmonooleate, glyceroldioleate; [0423] 1e) alcohol alcoxylates, preferably C10-18 alcohols, more preferably C10-C13 alcohols, most preferably C10 alcohols, from 5 to 80 oxyethylene units, more preferably from 5 to 40 oxyethylene units, most preferred from 7 to 14 oxyethylene units; [0424] 1f) alkyl glucosides having an alkyl chain length C5-C15 and 1 to 10 glucoside units, and
wherein the at least one category 2 additive is selected from at least one group of additives 2a) to 2c) [0425] 2a) polyethylene glycol-grafted polyacrylates, and salts thereof, preferably sodium salts thereof; [0426] 2b) ethoxylated polyethyleneimines; [0427] 2c) sodium lauryl ether sulfate, preferably sodium dodecylpoly(oxyethylene)sulfates with 1 to 50 oxyethylene units, preferably with 1 to 7 oxyethylene units, most preferred with 1 to 4 oxyethylene units,
wherein the category 3 additive belongs to a group of additives 3a) to 3g) [0428] 3a) lignosulfonates; [0429] 3b) alkylbenzene sulfonates and salts thereof; [0430] 3c) alkylnaphthalene sulfonates and salts thereof; [0431] 3e) napthalene sulfonic acid co-condensates; [0432] 3f) alkyl sulfonates and salts thereof; [0433] 3g) fatty alcohol sulfates and salts thereof, preferably sodium lauryl sulfate.

[0434] Embodiment 2: A spraying liquid according to embodiment 1, wherein the at least one category 1 additive of the groups 1a) to 1f) is selected from: [0435] for group 1a): poloxamers; [0436] for group 1b) statistical copolymers of polyvinyl pyrrolidone and polyvinyl acetate; [0437] for group 1c) polyoxyethylene sorbitol oleic acid esters with 20 to 40 oxyethylene units; [0438] for group 1d) fatty acid mono- and diesters of glycerol and polyglycerol [0439] for group 1e) alcohol alcoxylates with 5 to 80 oxyethylene units; [0440] for group 1f) alkyl polyglucosides having an alkyl chain length C8-C10 and mono- and diglucosides with an overall polymerization degree of 1.5.

[0441] Embodiment 3: A spraying liquid according to embodiment 1 or 2, wherein the at least one category 1 additive of the groups 1a) to 1f) is selected from: [0442] for group 1a) from polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol, polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol, polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, or polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol; [0443] for group 1b) polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol, or polyvinylpyrrolidone-polyvinylacetate statistical copolymer; [0444] for group 1c) polyoxyethylene (40) sorbitol hexaoleate, sorbitol ethoxylate esters, poly oxyethylene (20) sorbitan monolaurate, poly(oxyethylene) (20) sorbitan monooleate, sorbitan monooleate or sorbitol ethoxylate ester of polymerized fatty acid; [0445] for group 1d) mixtures of polyglycerolesters and fatty acid esters, or glycerolmon/diooleat; [0446] for group 1e) alkyl polyethylene glycol ether from C10-Guerbet alcohol, HLB 13, alkyl polyethylene glycol ether from C10-Guerbet alcohol, HLB 16, polyoxyethylene (23) lauryl ether or alkyl polyethylene glycol ether from C16 C18 fatty alcohol, HLB 18.5; [0447] for group 1f) alkyl polyglucoside.

[0448] Embodiment 4: A spraying liquid according to embodiment 3, wherein the at least one category 1 additive is [0449] a. a mixture of polyglycerolesters and fatty acid esters, [0450] b. a polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol, [0451] c. a polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, [0452] d. a polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol, [0453] e. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol, [0454] f. a polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol, [0455] g. a poly(oxyethylene) (20) sorbitan monooleate, [0456] h. a sorbitan monooleate, [0457] i. an alkyl polyglucoside, or [0458] g. a mixture of at least two category 1 additives selected from a., b., c., d. e., f., g., h. or i.

[0459] Embodiment 5: A spraying liquid according to embodiment 3, wherein the at least one category 2 additive of the groups 2a) to 2c) is selected from: [0460] for group 2a) methyl-methacrylate graft copolymer, [0461] for group 2b) ethoxylated cationic polyethyleneimine, [0462] for group 2c) sodium lauryl ether sulfate.

[0463] Embodiment 6: A spraying liquid according to embodiments 4 or 5, wherein the at least one category 1 additive is selected from: [0464] a. a mixture of polyglycerolesters and fatty acid esters, [0465] b. a polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol, [0466] c. a polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, [0467] d. a polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol, [0468] e. or a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol, or [0469] f. an alkyl polyglucoside, [0470] g. a polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol, and the category 2 additive is selected from [0471] h. a methyl-methacrylate graft copolymer, [0472] i. ethoxylated cationic polyethyleneimine or [0473] j. sodium laurylethersulfate,
or the category 1 and category 2 additives are a mixture of at least a one category 1 additive of a., b., c., d., e, f, or g. and at least one category 2 additive of h., i. or j.

[0474] Embodiment 7: A spraying liquid according to any one of embodiments 4 to 6, comprising: [0475] a. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives, [0476] b. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives, [0477] c. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additives, [0478] d. at least one poly(oxyethylene) (20) sorbitan monooleate and at least one sorbitan monooleate, or [0479] e. at least one polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol and at least one a polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol as category 1 additives.

[0480] Embodiment 8: A spraying liquid according to embodiment 6 or 7, comprising: [0481] a. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives and at least one sodium laurylethersulfate as category 2 additive [0482] b. at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additive and at least one methyl-methacrylate graft copolymer as category 2 additive, [0483] c. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol as category 1 additive and at least one methyl-methacrylate graft copolymer as category 2 additive, [0484] d. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives and at least one methyl-methacrylate graft copolymer as category 2 additive, [0485] e. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additives and at least one methyl-methacrylate graft copolymer as category 2 additive, [0486] f. at least one polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additive and at least one methyl-methacrylate graft copolymer as category 2 additive, or [0487] g. at least one alkyl polyglucoside as category 1 additive and at least one methyl-methacrylate graft copolymer as category 2 additive.

[0488] Embodiment 9: A spraying liquid according to any one of embodiments 4 to 8, comprising: [0489] a. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives, or [0490] b. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a mixture of polyglycerolesters and fatty acid esters as category 1 additives, [0491] c. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additives.

[0492] Embodiment 10: A spraying liquid according to any one of embodiments 6 to 8, comprising: [0493] a. polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives and a sodium laurylethersulfate as category 2 additive, [0494] b. a polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additive and a methyl-methacrylate graft copolymer as category 2 additive, [0495] c. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol as category 1 additive and a methyl-methacrylate graft copolymer as category 2 additive, [0496] d. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a mixture of polyglycerolesters and fatty acid esters as category 1 additives and a methyl-methacrylate graft copolymer as category 2 additive, [0497] e. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additives and a methyl-methacrylate graft copolymer as category 2 additive, [0498] f. a polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250) as category 1 additive and a methyl-methacrylate graft copolymer as category 2 additive, or [0499] g. an alkyl polyglucoside as category 1 additive and a methyl-methacrylate graft copolymer as category 2 additive.

[0500] Embodiment 11: A spraying liquid according to any one of embodiments 1 to 10, additionally comprising a category 3 additive, in a total amount of more than 0 g per hectare to 80 g per hectare, and being selected from at least one group of additives 3a) to 3g) [0501] 3a) lignosulfonates; [0502] 3b) alkylbenzene sulfonates and salts thereof; [0503] 3c) alkylnaphthalin sulfonates and salts thereof; [0504] 3e) napthalene sulfonic acid co-condensates; [0505] 3f) alkyl sulfonates and salts thereof; [0506] 3g) fatty alcohol sulfates and salts thereof, preferably sodium lauryl sulfate.

[0507] Embodiment 12: Kit of at least two parts to prepare a spraying liquid according to any one of the embodiments 1 to 11, wherein the biomass comprising spores of fusaricidin producing bacteria are provided in a first concentrated form and at least one of the category 1 additive and/or category 2 additive and/or category 3 additive are provided in at least one further concentrated forms and wherein the relative amounts of the biomass comprising spores of fusaricidin producing bacteria and the relative amounts the category 1 additive and/or the category 2 additive and/or the category 3 additive in the kit are adapted to provide the amounts described in any one of the embodiments 1 to 11.

[0508] Embodiment 13: Kit of parts according to embodiment 12, wherein the spraying liquid is prepared to be used in a method to control phytopathogenic fungi.

[0509] Embodiment 14: Method to control phytopathogenic fungi wherein a spraying liquid according to any one of embodiments 1 to 11 is sprayed on plants.

[0510] Embodiment 15: Method to control phytopathogenic fungi according to embodiment 14, wherein the spraying liquid is sprayed in an amount of about 100 liter to about 1000 liter per hectare and the spraying liquid comprises biomass comprising spores of fusaricidin producing bacteria in an amount of 0.005% w/w to 2% w/w and the category 1 additive and/or the category 2 additive in a total amount of 0.005% w/w to 2% w/w.

[0511] Embodiment 16: Method to control phytopathogenic fungi according to embodiment 15, wherein the spraying liquid comprises a category 3 additive in an amount of 0.0001% w/w to 0.08% w/w.

[0512] Embodiment 17: Method to control phytopathogenic fungi according to any one of embodiments 14 to 17, wherein the plants are selected from maize, small-grain cereals, cotton, canola, sunflowers, tobacco, peanuts, vegetables, grapevines, leafy vegetables, root & tuber vegetables, onions, strawberries, blueberries, strawberry, black currant, white currant, red currant, blackberry, raspberry, tomato, pepper, chilli, cucumber, aubergine, melons, watermelons, cucumbers, tomatoes, peppers & aubergines, herbs, turf and ornamentals.

[0513] Embodiment 18: Method to control phytopathogenic fungi according to any one of embodiments 14 to 17, wherein the phytopathogenic fungi is Alternaria spp., Botrytis cinerea or Phytophthora infestans.

[0514] Embodiment 19: A solid or liquid formulation, preferably a wettable powder (WC) or suspension concentrate (SC) formulation, comprising: [0515] a. 5% w/w to 50% w/w biomass comprising spores of fusaricidin producing bacteria, [0516] b. 5% w/w to 50% w/w of at least one category 1 additive or at least one category 2 additive or a mixture of at least one category 1 additive or at least one category 2 additive, and [0517] c. less than 2% w/w of category 3 additives,
wherein the at least one category 1 additive is selected from at least one group of additives 1a) to 1f): [0518] 1a) poloxamers, polyethylene glycol, polypropylene glycol or polyethylene glycol block polymers with a molar mass of approximately 950 to 6500 g/mol and a polyethylene oxide content of 10% to 50% w/w; [0519] 1b) polyvinylpyrolidines, polyvinylacetates and statistical and block copolymers of polyvinyl pyrrolidone and polyvinyl acetate, all with a molar mass of approximately 9000 to 70000 g/mol; [0520] 1c) polyoxyethylene sorbitol fatty acid esters (“polysorbates”), having a fatty acid chain with a length of 10 to 20 carbon atoms, preferably 12 to 18 carbon atoms, most preferably dodecanoic acid and oleic acid and linoleic acid, from 10 to 150 oxyethylene units, preferably 20 to 80 oxyethylene units, most preferred 20 to 40 oxyethylene units; [0521] 1d) fatty acid glycerol esters, fatty acid mono- and diesters of glycerol and polyglycerol, glycerolmonooleate, glyceroldioleate, polyglycerol fatty acid ester; [0522] 1e) alcohol alcoxylates, preferably C10-18 alcohols, more preferably C10-C13 alcohols, most preferably C10 alcohols, from 5 to 80 oxyethylene units, more preferably from 5 to 40 oxyethylene units, most preferred from 7 to 14 oxyethylene units; [0523] 1f) alkyl glucosides having an alkyl chain length C5-C15 and 1 to 10 glucoside units, and the at least one category 2 additive is selected from at least one group of additives 2a) to 2c) [0524] 2a) polyethylene glycol-grafted polyacrylates, and salts thereof, preferably sodium salts thereof; [0525] 2b) ethoxylated polyethyleneimines; [0526] 2c) sodium lauryl ether sulfate, preferably sodium dodecylpoly(oxyethylene)sulfates with 1 to 50 oxyethylene units, preferably with 1 to 7 oxyethylene units, most preferred with 1 to 4 oxyethylene units, [0527] and the category 3 additives are selected from the groups of additives 3a) to 3g) [0528] 3a) lignosulfonates; [0529] 3b) alkylbenzene sulfonates and salts thereof; [0530] 3c) alkylnaphthalene sulfonates and salts thereof; [0531] 3e) apthalene sulfonic acid co-condensates; [0532] 3f) alkyl sulfonates and salts thereof; [0533] 3g) fatty alcohol sulfates and salts thereof, preferably sodium lauryl sulfate.

[0534] Embodiment 20: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation, according to embodiment 19, wherein the at least one category 1 additive of the groups 1a) to 1f) is selected from: [0535] for group 1a): poloxamers; [0536] for group 1b) statistical copolymers of polyvinyl pyrrolidone and polivinyl acetate; [0537] for group 1c) polyoxyethylene sorbitol oleic acid esters with 20 to 40 oxyethylene units [0538] for group 1d) fatty acid mono- and diesters of glycerol and polyglycerol [0539] for group 1e) alcohol alcoxylates with 5 to 80 oxyethylene units, [0540] for group 1f) alkyl polyglucosides having an alkyl chain length C8-C10 and mono- and diglucosides with an overall polymerization degree of 1.5.

[0541] Embodiment 21: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation, according to embodiment 19 or 20, wherein the at least one category 1 additive of the groups 1a) to 1f) is selected from: [0542] for group 1a) from polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol, polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol, polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol or polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol; [0543] for group 1b) polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol, or polyvinylpyrrolidone-polyvinylacetate statistical copolymer; [0544] for group 1c) polyoxyethylene (40) sorbitol hexaoleate, sorbitol ethoxylate esters, poly oxyethylene (20) sorbitan monolaurate, poly(oxyethylene) (20) sorbitan monooleate, sorbitan monooleate or sorbitol ethoxylate ester of polymerized fatty acid; [0545] for group 1d) mixtures of polyglycerolesters and fatty acid esters or glycerolmon/diooleat; [0546] for group 1e) alkyl polyethylene glycol ether from C10-Guerbet alcohol, HLB 13, alkyl polyethylene glycol ether from C10-Guerbet alcohol, HLB 16, polyoxyethylene (23) lauryl ether or alkyl polyethylene glycol ether from C16 C18 fatty alcohol, HLB 18.5; [0547] for group 1f) alkyl polyglucoside.

[0548] Embodiment 22: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation, according to any one of embodiments 19 to 21, wherein the at least one category 1 additive is [0549] a. at least one mixture of polyglycerolesters and fatty acid esters, [0550] b. at least one polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol, [0551] c. at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, [0552] d. at least one polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol, [0553] e. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol, [0554] f. at least one polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol, [0555] g. at least one alkyl polyglucoside, or [0556] h. a mixture of at least two category 1 additives selected from a., b., c., d., e., f., or g.

[0557] Embodiment 23: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation, according to any one of embodiments 19 to 22, wherein the at least one category 2 additive of the groups 2a) to 2c) is selected from: [0558] for group 2a) methyl-methacrylate graft copolymer, [0559] for group 2b) ethoxylated cationic polyethyleneimine, or [0560] for group 2c) sodium lauryl ether sulfate.

[0561] Embodiment 24: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiments 19 to 23, wherein the at least one category 1 additive is selected from: [0562] a. at least one mixture of polyglycerolesters and fatty acid esters, [0563] b. at least one polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol, [0564] c. at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, [0565] d. at least one polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol, [0566] e. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol, [0567] f. at least one polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol or [0568] g. at least one alkyl polyglucoside, [0569] and the category 2 additive is selected from [0570] h. at least one methyl-methacrylate graft copolymer, [0571] i. at least one ethoxylated cationic polyethyleneimine or [0572] j. at least one sodium laurylethersulfate, [0573] or the category 1 and category 2 additives are a mixture of at least one category 1 additive of a., b., c., d., e., f., or g. and at least one category 2 additive of h., i. or j.

[0574] Embodiment 25: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiment 19 to 24, comprising: [0575] a. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives, [0576] b. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives, [0577] c. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additives, [0578] d. at least one poly(oxyethylene) (20) sorbitan monooleate and at least one sorbitan monooleate as category 1 additives, or [0579] e. at least one polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol and at least one polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol as category 1 additives.

[0580] Embodiment 26: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiments 19 to 25, comprising: [0581] a. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives and at least one sodium laurylethersulfate as category 2 additive, [0582] b. at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additive and at least one methyl-methacrylate graft copolymer as category 2 additive, [0583] c. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol as category 1 additive and at least one methyl-methacrylate graft copolymer as category 2 additive, [0584] d. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives and at least one methyl-methacrylate graft copolymer as category 2 additive, [0585] e. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additives and at least one methyl-methacrylate graft copolymer as category 2 additive, [0586] f. at least one polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additive and at least one methyl-methacrylate graft copolymer as category 2 additive, or [0587] g. at least one alkyl polyglucoside as category 1 additive and at least one methyl-methacrylate graft copolymer as category 2 additive.

[0588] Embodiment 27: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiments 19 to 26, comprising: [0589] a. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives, or [0590] b. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a mixture of polyglycerolesters and fatty acid esters as category 1 additives, [0591] c. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additives, or [0592] d. a polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol and a polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol.

[0593] Embodiment 28: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiments 19 to 27, comprising: [0594] a. polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives and a sodium laurylethersulfate as category 2 additive, [0595] b. a polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additive and a methyl-methacrylate graft copolymer as category 2 additive, [0596] c. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol as category 1 additive and a methyl-methacrylate graft copolymer as category 2 additive, [0597] d. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a mixture of polyglycerolesters and fatty acid esters as category 1 additives and a methyl-methacrylate graft copolymer as category 2 additive, [0598] e. a polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and a polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additives and a methyl-methacrylate graft copolymer as category 2 additive, [0599] f. a polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol as category 1 additive and a methyl-methacrylate graft copolymer as category 2 additive, or [0600] g. an alkyl polyglucoside as category 1 additive and a methyl-methacrylate graft copolymer as category 2 additive.

[0601] Embodiment 29: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiments 19 to 28, additionally comprising a category 3 additive, in a total amount of more than 0% w/w to 2% w/w, and being selected from at least one group of additives 3a) to 3g) [0602] 3a) lignosulfonates; [0603] 3b) alkylbenzene sulfonates and salts thereof; [0604] 3c) alkylnaphthalene sulfonates and salts thereof; [0605] 3e) napthalene sulfonic acid co-condensates; [0606] 3f) alkyl sulfonates and salts thereof; [0607] 3g) fatty alcohol sulfates and salts thereof, preferably sodium lauryl sulfate.

[0608] Embodiment 30: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiment 19 to 29 comprising [0609] a. 5% w/w to 50% w/w biomass comprising spores of fusaricidin producing bacteria, [0610] b. 2% w/w to 50% w/w of a category 1 additive, [0611] c. 0% w/w to 50% w/w of a category 2 additive, [0612] d. 0 w/w to 2% w/w of a category 3 additive, and [0613] e. 20% w/w to 75% w/w of auxiliaries.
wherein the total amounts of a., b., c., d., and e. are selected to result in a total amount of 100% w/w.

[0614] Embodiment 31: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiments 19 to 30 comprising [0615] a. 10% w/w to 40% w/w biomass comprising spores of fusaricidin producing bacteria, [0616] b. 10% w/w to 40% w/w of a category 1 additive, [0617] c. 2.5% w/w to 20% w/w of a category 2 additive, [0618] d. 0 w/w to 2% w/w of a category 3 additive, and [0619] e. 20% w/w to 75% w/w of auxiliaries. [0620] wherein the total amounts of a., b., c., d., and e. are selected to result in a total amount of 100% w/w.

[0621] Embodiment 32: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiments 19 to 31 comprising [0622] a. 10% w/w to 35% w/w biomass comprising spores of fusaricidin producing bacteria, [0623] b. 3% w/w to 40% w/w of a category 1 additive, [0624] c. 0% w/w to 15% w/w of a category 2 additive, [0625] d. 0 w/w to 2% w/w of a category 3 additive, and [0626] e. 20% w/w to 75% w/w of auxiliaries.
wherein the total amounts of a., b., c., d. and e. are selected to result in a total amount of 100% w/w.

[0627] Embodiment 33: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiments 19 to 33 comprising [0628] a. 15% w/w to 35% w/w biomass comprising spores of fusaricidin producing bacteria, [0629] b. 3% w/w to 20% w/w of a category 1 additive, [0630] c. 0% w/w to 7% w/w of a category 2 additive, [0631] d. 0% w/w to 1.5% w/w of a category 3 additive, and [0632] e. 20% w/w to 75% w/w of auxiliaries.
wherein the total amounts of a., b., c., d. and e. are selected to result in a total amount of 100% w/w.

[0633] Embodiment 34: A solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation according to any one of embodiments 19 to 33 comprising [0634] a. 15% w/w to 40% w/w biomass comprising spores of fusaricidin producing bacteria, [0635] b. 3% w/w to 40% w/w of a category 1 additive, [0636] c. 2% w/w to 15% w/w of a category 2 additive, [0637] d. 0% w/w of a category 3 additive, and [0638] e. 30% w/w to 75% w/w of auxiliaries.
wherein the total amounts of a., b., c., d. and e. are selected to result in a total amount of 100% w/w.

[0639] Embodiment 35: A solid formulation, preferably a wettable powder (WP) formulation, according to any one of embodiments 19 to 34, comprising 2% w/w to 50% w/w of a category 1 additive, wherein the category 1 additive is a combination of [0640] a. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol as category 1 additives, [0641] b. at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives, [0642] c. at least one poly(oxyethylene) (20) sorbitan monooleate and at least one sorbitan monooleate, or [0643] d. at least one polyethylene—polypropylene glycol block polymer with 10% ethyleneoxide, 850 g/mol and at least one polyvinylpyrrolidone with an average molar mass of maximally 50 000 g/mol, as category 1 additives.

[0644] Embodiment 36: A solid formulation, preferably a wettable powder (WP) formulation, according embodiment 35, comprising 10% w/w to 50% w/w of a category 1 additive wherein the category 1 additive is a combination of [0645] a. 5% w/w to 45% w/w of at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and [0646] b. 5% w/w to 45% w/w of at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol.

[0647] Embodiment 37: A solid formulation, preferably a wettable powder (WP) formulation, according embodiment 35, comprising 10% w/w to 50% w/w of a category 1 additive wherein the category 1 additive is a combination of [0648] a. 9% w/w to 45% w/w of at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and 1% w/w to 10% w/w of at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives.

[0649] Embodiment 38: A solid formulation, preferably a wettable powder (WP) formulation, according embodiment 35, comprising as category 1 additives: [0650] a. 1.0% w/w to 20% w/w of at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and [0651] b. 10% w/w to 19% w/w of at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol.

[0652] Embodiment 39: A solid formulation, preferably a wettable powder (WP) formulation, according embodiment 35, comprising: [0653] a. 7% w/w to 45% w/w of at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and 3% w/w to 10% w/w of at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives.

[0654] Embodiment 40: A solid formulation, preferably a wettable powder (WP) formulation, according to any one of embodiments 36 to 38, comprising as category 1 additives: [0655] a. 1.0% w/w to 20% w/w of at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and [0656] b. 10% w/w to 19% w/w of at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol and
comprising as category 2 additive 0.5% w/w to 2% w/w of at least one sodium laurylethersulfate.

[0657] Embodiment 41: A solid formulation, preferably a wettable powder (WP) formulation, according to embodiment 40, comprising: [0658] a. 1% w/w to 20% w/w of at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and [0659] b. 10% w/w to 19% w/w of at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, and [0660] c. 0.5% w/w to 14% w/w of at least one methyl-methacrylate graft copolymer.

[0661] Embodiment 42: A solid formulation, preferably a wettable powder (WP) formulation, according to embodiment 40, comprising: [0662] a. 1% w/w to 20% w/w of at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and [0663] b. 10% w/w to 19% w/w of at least one polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, and [0664] c. 0.8% w/w to 8% w/w of at least one methyl-methacrylate graft copolymer.

[0665] Embodiment 43: A solid formulation, preferably a wettable powder (WP) formulation, according to embodiment 35, comprising: [0666] a. 7% w/w to 45% w/w of at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol, [0667] b. 3% w/w to 10% w/w of at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives, and [0668] c. 0.8% w/w to 14% w/w of at least one methyl-methacrylate graft copolymer. [0669] 2. A solid formulation, preferably a wettable powder (WP) formulation, according to claim 43, comprising: [0670] a. 7% w/w to 45% w/w of at least one polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol, [0671] b. 3% w/w to 10% w/w of at least one mixture of polyglycerolesters and fatty acid esters as category 1 additives, and [0672] c. 0.8% w/w to 8% w/w of at least one methyl-methacrylate graft copolymer.

[0673] Embodiment 44: A solid formulation, preferably a wettable powder (WP) formulation, according to any one of embodiments 35 to 44, packed in vacuum sealed multifoil or mylar pouches, or pouches made of water-soluble polymers, e.g. polyvinyl alcohol pouches.

[0674] Embodiment 45: A liquid formulation, preferably suspension concentrate (SC) formulation, according to at least one of embodiments 19 to 33, comprising: [0675] a. 2% w/w to 15% w/w of at least one category 1 additive selected from polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol, polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol and/or a mixture of polyglycerolesters and fatty acid esters, or a mixture of at least two of these, and [0676] b. 1% w/w to 15% w/w of at least one category 2 additive selected from methyl-methacrylate graft copolymer.

[0677] Embodiment 46: A liquid formulation, preferably suspension concentrate (SC) formulation, according to at least one of embodiments 19 to 33 and 45, comprising: [0678] a. 3% w/w to 10% w/w of a category 2 additive methyl-methacrylate graft copolymer and [0679] b. at least one category 1 additive selected from the alternatives [0680] i. 3% w/w to 10% w/w polyethylene—polypropylene glycol block polymer with 20% ethyleneoxide, 3250 g/mol, [0681] ii. 2% w/w to 15% w/w of at least one category 1 additive selected from polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol, [0682] iii. 2% w/w to 10% w/w of at least one category 1 additive selected from polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and 1% w/w to 5% w/w of a mixture of polyglycerolesters and fatty acid esters, [0683] iv. 3% w/w to 10% w/w of at least one category 1 additive selected from polyethylene—polypropylene glycol block polymer with 50% ethyleneoxide, 1750 g/mol and 3% w/w to 10% w/w of polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol.

[0684] Embodiment 47: A liquid formulation, preferably suspension concentrate (SC) formulation, according to at least one of embodiments 19 to 33 and 46 or 47, comprising [0685] 35% w/w to 45% w/w of glycerol, propane-1,2-diol (propylen glycol), butane-1,4-diol, butane-1,3-diol, pentane-1,5-diol, hexane-1,6-diol, 2-methyl-2,4-pentanediol (hexylene glycol), heptan-1,7-diol, 2-ethylhexan-1,3-diol, polyethylene glycol (PEG) with a mean molecular weight of 150 g/mol to 450 g/mol, polypropylene glycol (PPG) with a mean molecular weight of 200 to 500 g/mol, propylene glycol methyl ether, dipropylene glycol methyl ether, tripropylene glycol methyl ether, dipropylene glycol dimethyl ether, propylene glycol n-propyl ether, diethylene glycol monobutylether, 2-hydroxypropane-1,2,3-tricarboxylate (triethyl citrate), or N,N-dimethyl lactamide or a mixture of at least two of these.

[0686] Embodiment 48: A liquid formulation, preferably suspension concentrate (SC) formulation, according to at least one of embodiments 19 to 33 and 46 or 47, comprising 35% w/w to 45% w/w of polyethylene glycol (PEG), propane-1,2-diol, 2-methyl-2,4-pentanediol or triethyl citrate or a mixture of at least two of these.

[0687] Embodiment 49: A liquid formulation, preferably suspension concentrate (SC) formulation, according to at least one of embodiments 19 to 33 and 46, comprising: [0688] a. 6% w/w to 15% w/w of the category 2 additive methyl-methacrylate graft copolymer and [0689] b. 2% w/w to 8% w/w of the category 1 additive polyethylene—polypropylene glycol block polymer with 40% ethyleneoxide, 3250 g/mol.

[0690] Embodiment 50: A liquid formulation, preferably suspension concentrate (SC) formulation, according to at least one of embodiments 19 to 33 and 46, comprising: [0691] a. 6% w/w to 15% w/w of the category 2 additive methyl-methacrylate graft copolymer and [0692] b. 3% w/w to 8% w/w of the category 3 additive alkyl polyglucoside.

[0693] Embodiment 51: A liquid formulation, preferably suspension concentrate (SC) formulation, according to at least one of embodiment 19 to 33 and 46 or 47, comprising 70% w/w to 85% w/w of glycerol, propane-1,2-diol (propylen glycol), butane-1,4-diol, butane-1,3-diol, pentane-1,5-diol, hexane-1,6-diol, 2-methyl-2,4-pentanediol (hexylene glycol), polyethylene glycol (PEG) with a mean molecular weight of 150 g/mol to 450 g/mol, polypropylene glycol (PPG) with a mean molecular weight of 200 to 500 g/mol, propylene glycol methyl ether, dipropylene glycol methyl ether, tripropylene glycol methyl ether, dipropylene glycol dimethyl ether, propylene glycol n-propyl ether, diethylene glycol monobutylether, triethyl 2-hydroxypropane-1,2,3-tricarboxylate (triethyl citrate), tributyl 2-hydroxypropane-1,2,3-tricarboxylat (tributyl citrate), or N,N-dimethyl lactamide or a mixture of at least two of these.

[0694] Embodiment 52: A liquid formulation, preferably suspension concentrate (SC) formulation, according to embodiments 50 or 51, comprising 75% w/w to 85% w/w of polyethylene glycol (PEG), propane-1,2-diol, 2-methyl-2,4-pentanediol, triethyl citrate or tributyl citrate or a mixture of at least two of these, preferably polyethylene glycol (PEG), 2-methyl-2,4-pentanediol, triethyl citrate or tributyl citrate or a mixture of at least two of these, even more preferred polyethylene glycol (PEG), 2-methyl-2,4-pentanediol or triethyl citrate, or a mixture of at least two of these.

[0695] Embodiment 53: Method to control phytopathogenic fungi on plants comprising [0696] a. providing at least one solid or liquid formulation, preferably a wettable powder (WP) or suspension concentrate (SC) formulation, according to any one of embodiments 19 to 52 in a total amount of 0.5 kg to 4 kg per hectare or a kit of parts according to embodiments 12 or 13, [0697] b. adding water to a final amount of 100 liters to 1000 liters per hectare, and [0698] c. spraying the resulting spraying liquid on plants.

[0699] Embodiment 54: Process for making solid formulation, preferably a wettable powder (WP) formulation, comprising: [0700] a. 5.0% w/w to 50% w/w biomass comprising spores of fusaricidin producing bacteria, [0701] b. 5.0% w/w to 50% w/w of a category 1 additive, [0702] c. 0% w/w to 20% w/w of a category 2 additive, [0703] d. 0 w/w to 2% w/w of a category 3 additive, and [0704] e. 30% w/w to 50% w/w of auxiliaries.
wherein the total amounts of a, b, c, d, and e are selected to result in a total amount of 100% w/w,
comprising the following steps: [0705] A. providing biomass comprising spores of fusaricidin producing bacteria, [0706] B. dispersing said biomass in a continuous phase comprising, [0707] C. adding at least one category 1 additive, [0708] D. optionally adding at least one category 2 additive, [0709] E. optionally adding at least one category 3 additive, [0710] F. optionally adding auxiliaries, [0711] G. drying the biomass containing slurry preferably by spray-drying, vacuum-drum drying, fluidized-bed drying or spray-granulation, [0712] wherein steps C., D., E. and F. can be carried out at any time during the process.

[0713] Embodiment 55: Process for making a suspension concentrate (SC) formulation comprising: [0714] d. providing biomass comprising spores of fusaricidin producing bacteria, [0715] e. dispersing said biomass by shear or mechanical energy input, e.g. in a rotor-stator colloid mill or a bead mill in a continuous phase comprising, [0716] f. adding at least one category 1 additive, [0717] g. optionally adding at least one category 2 additive, [0718] h. optionally adding at least one category 3 additive, [0719] i. optionally adding auxiliaries, [0720] j. wherein steps c), d), e) and f) can be carried out at any time during the process.

EXAMPLES

[0721] Materials:

TABLE-US-00002 Product Name Function Category Chemical name Wettol ® D3 Wetting Agent, naphthalenesulfonic acid-formaldehyde- Dispersant polycondensate, sodium salt Polyfon ® H Dispersant Kraft-Lignin-Sulfonate, aliphatic sulfonate, low Sulfonate, high Mw Polyfon ® F Dispersant Kraft-Lignin-Sulfonate, aliphatic sulfonate, high Sulfonate, high Mw Borresperse Na Dispersant Lignosulfonic acid, sodium salt Atlox ™ 4913 Dispersant Methyl methacrylate graft copolymer, anionic (solution) Atlox ™ 4915 Dispersant Low charge PEG-quat-C15-triacid Atlox ™ 4916 Dispersant Sorbitol ethoxylate ester of polymerized fatty acid Borresperse Ca Dispersant Lignosulfonic acid, calcium salt Vanisperse ® CB Dispersant Lignosulfonic acid, sodium salt Wettol ® D1 Wetting agent, Benzenesulfonic acid-formaldehyde- dispersant phenol-urea-polycondensate, sodium salt Tensiofix ® BCZ Wetting agent Sodium lauryl sulfate Agnique ® SLES Wetting agent Sodium lauryl ether sulfate (solution) 370 Agnique ® PG 8107 Wetting agent, Alkyl polyglucoside (solution) dispersant Tween ® 80 Wetting agent, Poly(oxyethylene) (20) sorbitan dispersant monooleate Sokalan ® HP50 Dispersant Polyvinylpyrrolidone, Mw 40 000 Pluronic ® PE 3100 Dispersant Polyethylene glycol - polypropylene glycol block polymer, approx. 10% ethylene glycol Pluronic ® PE 10500 Dispersant Polyethylene glycol - polypropylene glycol block polymer, approx. 50% ethylene glycol Pluronic ® PE 6200 Dispersant Polyethylene glycol - polypropylene glycol block polymer, approx. 20% ethylene glycol Pluronic ® PE 6400 Dispersant Polyethylene glycol - polypropylene glycol block polymer, approx. 40% ethylene glycol Luvitec ® VA 64 Dispersant Statistical copolymer of vinyl pyrrolidone and vinyl acetate Break-Thru ® SP133 Wetting agent, Mixture of polyglycerolesters, fatty dispersant acid esters Span ™ 80 Dispersant Sorbitan monooleate Tween ® 20 Dispersant Poly oxyethylene (20) sorbitan monolaurate Break-Thru ® SF Dispersant Aqueous solution of sophorolipid 420 Brij ®-23 Dispersant Polyoxyethylene (23) lauryl ether Atlas ™ G-1086 Dispersant Polyoxyethylene (40) sorbitol hexaoleate Atlas ™ G-1096 Dispersant Sorbitol ethoxylate esters Dispersogen PSL Dispersant Polyacrylates graft copolymer, nonionic 100 Sokalan ® HP20 Dispersant Polyethyleneimine, ethoxylated, cationic Break-Thru ® EM Wetting agent Polyglycerol oleic acid esters, O5 HLB 5.5 Lutensol ® XL70 Wetting agent Alkyl polyethylene glycol ether from C10-Guerbet alcohol, HLB 13 Lutensol ® XL140 Dispersant Alkyl polyethylene glycol ether from C10-Guerbet alcohol, HLB 16 Lutensol ® AT 80 Dispersant Alkyl polyethylene glycol ether from C16 C18 fatty alcohol, HLB 18.5 Lutensol ® AT 11 Dispersant Alkyl polyethylene glycol ether from C16 C18 fatty alcohol, HLB 13 Sipernat ® 310 Insoluble filler Finely ground precipitated silica Pluriol ® E200 Diluent Polyethylene glycol, molecular weight 200 Dalton Sunflower oil Diluent Sunflower oil, refined Ammonium sulfate Soluble filler Ammonium sulfate Celite ® 266 Insoluble filler Diatomaceous earth Sipernat ® 310 Insoluble filler Precipiated silica Magensium sulfate - Soluble filler Magensium sulfate - heptahydrate heptahydrate Potassium phosphate, Soluble filler Potassium phosphate, dibasic dibasic Potassium phosphate, Soluble filler Potassium phosphate, monobasic monobasic Wacker ® Silicone Antifoam Polydimethylsiloxane emulsion SRE-PFL Break-Thru ® S301 Wetting agent Siloxane surfactant Aerosil ® 200 Anti-settling agent Fumed silica, hydrophilic Wettol ® D3 Wetting Agent, naphthalenesulfonic acid-formaldehyde- Dispersant polycondensate, sodium salt Polyfon ® H Dispersant Kraft-Lignin-Sulfonate, aliphatic sulfonate, low Sulfonate, high Mw

Example 1: Greenhouse Tests of Paenibacillus sp. Strain Lu17007 Powder Tank-Mixed with Different Additive Combinations

[0722] Paenibacillus Technical Active Ingredient

[0723] Paenibacillus sp. strain Lu17007, isolated 1996 in Stuttgart (Germany), deposited with DSMZ under Accession No. DSM 26970 and disclosed in WO2016/020371, was grown in a complex medium to an OD of 25 to 50 and a spore content of 5×10.sup.7 CFU/ml to 5×10.sup.8 CFU/ml. The whole fermentation broth was washed by diafiltration. The resulting pellet was mixed with finely ground precipitated silica. This suspension was spray dried under nitrogen atmosphere at 150° C. to 160° C. inlet temperature and 80° C. to 95° C. outlet temperature.

[0724] The resulting fine powder of Paenibacillus sp. strain Lu17007 containing 9% precipitated silica (w/w with respect to the total dry mass of the resulting dry product) was used as technical active ingredient (TGAI) in the following experiments.

[0725] Wettable Powder Premix and Additive Tank-Mix Solutions

[0726] Paenibacillus sp. strain Lu17007 TGAI was dry mixed with fillers to create wettable powder premixes. Wettable powder premix P.11 consisted of 50% (w/w) Paenibacillus sp. strain Lu17007 TGAI, 45% Ammonium sulfate and additional 5% of finely ground precipitated silica. WP premix P.12 consisted of 50% Paenibacillus sp. strain Lu17007 TGAI, 25% Ammonium sulfate and 25% diatomaceous earth as fillers.

[0727] Dilution media for spray suspensions were prepared by adding wetting agents and dispersants to tap water and homogenizing. Dilution medium A.1 contained 0.05% (w/w) sodium salt of naphthalene sulfonic acid-formaldehyde polycondensate and 0.01% sodium lauryl sulfate. Dilution medium N.1 contained 0.05% polyoxyethylene (20) sorbitan monooleate and 0.05% sorbitan monooleate. The wettable powder premixes P.11 and P.12 were dosed into the dilution media A.1 and N.1 respectively as shown in table 1, and shortly stirred to create homogeneous spray slurries.

[0728] Greenhouse Pot Trials

[0729] The fungicidal activity of the resulting spray suspensions containing Paenibacillus sp. strain Lu17007 wettable powder and additives as listed in table 1, was compared in greenhouse pot trials at the BASF trial station in Limburgerhof/Germany. Tests were done against Phytophthora infestans and Alternaria Solani, both in tomatoes cv. Goldene Königin, and against Botrytis cinerea in pepper cv. Neusiedler Ideal. The plants were used in BBCH stage 12 (=2nd leaf unfolded). A spraying cabinet was used for the application on plants. All trials had 3 replicates and the plots were randomized. One day after the application of the products, the trials were inoculated by using a spore suspension of the pathogen (2E+5 spores/mL). Incubation took place in the greenhouse for 3 to 5 d at 21° C. and 90% rH before the evaluation of percent leaf damage was carried out.

TABLE-US-00003 TABLE 1 Antifungal activity of Paenibacillus sp. strain Lu17007 WP formulation combined with different additives as tank-mix (dose rate in % w/w). Dose rate WP Efficacy Efficacy Efficacy Spray premix (% Efficacy Efficacy Efficacy slurry/ WP w/w of total Dilution ALTESO BOTRCI PHYTIN treatment premix spray slurry) medium % % % 1 None 0 Tab water 0 0 0 2 Paenibacillus 0.044 Tab water 90 72 73 sp. strain Lu17007 TGAI 3 P.11 0.044 A.1 29 5 13 4 P.11 0.067 A.1 50 29 23 5 P.11 0.111 A.1 86 35 76 6 P.11 0.044 N.1 87 84 72 7 P.11 0.067 N.1 95 92 80 8 P.11 0.111 N.1 97 99 80 9 P.12 0.044 A.1 21 0 10 10 P.12 0.067 A.1 36 0 47 11 P.12 0.111 A.1 93 0 88 12 P.12 0.044 N.1 96 71 65 13 P.12 0.067 N.1 99 83 72

[0730] The treatments containing dilution medium N.1 with nonionic wetting agent and dispersant performed significantly better against Alternaria solani on tomato, Botrytis cinerea on pepper and Phytophtera infenstans on tomato at much lower active ingredient rate than treatments containing dilution medium A.1 with anionic wetting agent and dispersant.

Example 2: Greenhouse Pot Trials with Paenibacillus sp. Strain A Formulations from Concentrated Broth

[0731] Paenibacillus sp. strain A, a mutant of Paenibacillus sp. strain Lu17007, was grown in a complex medium to an OD of 25 to 50 and a spore content of 5×10.sup.7 CFU/ml to 5×10.sup.8 CFU/ml. The whole fermentation broth was run over a disc separator to create a pellet with 8.9% dry mass. The pellet fraction was mixed with formulation additives as listed in table 2.

TABLE-US-00004 TABLE 2 Liquid Paenibacillus sp. strain A formulations. Amount of ingredients is given in g/kg. Ingredient (g/kg) P.2 N.21 N.22 A.21 A.22 Paenibacillus sp. 562 562 562 562 562 strain A pellet Sipernat ® 310 112 112 Celite ® 266 304 225 304 Ammonium sulfate 0 304 180 Tensiofix ® BCZ 11 Polyfon ® F 56 Polyfon ® H 112 Borresperse Na 90 Pluronic ® PE 3100 56 56 Sokalan ® HP50 56 56 Tween ® 80 28 Sunflower oil 28 Wacker ® Silicone 22 SRE-PFL Water 562 34 34 34 34

[0732] The liquid Paenibacillus sp. strain A formulations of table 2 were diluted with tap water to the desired concentration as listed in

[0733] Table 3 for application in greenhouse pot trials against Alternaria solani on tomato, Botrytis cinerea on pepper and Phytophtera infenstans on tomato, as described in detail in example 1.

TABLE-US-00005 TABLE 3 Antifungal activity of liquid Paenibacillus sp. strain A formulations. Dose rate liquid premix Spray (% w/w of Efficacy Efficacy Efficacy slurry/ total spray Dilution ALTESO BOTRCI PHYTIN treatment Premix slurry) medium % % % 1 Control none Tab water 0 0 0 2 P.2 0.439 Tab water 46 3 3 3 P.2 0.878 Tab water 56 7 14 4 P.2 1.755 Tab water 84 13 57 5 N.21 0.439 Tab water 80 10 21 6 N.21 0.878 Tab water 96 73 46 7 N.21 1.755 Tab water 94 100 77 8 N.22 0.439 Tab water 80 15 46 9 N.22 0.878 Tab water 92 50 53 10 N.22 1.755 Tab water 99 91 77 11 A.21 0.439 Tab water 75 7 14 12 A.21 0.878 Tab water 85 13 32 13 A.21 1.755 Tab water 94 15 62 14 A.22 0.439 Tab water 68 20 3 15 A.22 0.878 Tab water 73 13 10 16 A.22 1.755 Tab water 87 60 17

[0734] The treatments containing formulations N.21 and N.22 with nonionic wetting agents and dispersants performed significantly better at the two highest dose rates against Botrytis cinerea and Phytophtera infenstans than the diluted Paenibacillus sp. strain A pellet alone or the treatments containing formulations A.21 and A.22 with anionic wetting agents and dispersants. For Alternaria solani, the effect was not so pronounced, since the infection level in this trial was extremely low (67.5%).

Example 3: Greenhouse Activity of Paenibacillus sp. Strain B Powder in Tank-Mix with Different Surface-Active Additives

[0735] Paenibacillus sp. strain B, a mutant of Paenibacillus sp. strain Lu17007, was grown was grown in a complex medium to an OD of 25 to 50 and a spore content of 5×10.sup.7 CFU/ml to 5×10.sup.8 CFU/ml. The whole fermentation broth was run over a disc separator to create a pellet with 2.3% dry mass. The pellet fraction was mixed with finely ground precipitated silica and the suspension was spray dried under nitrogen atmosphere with an inlet temperature of 170° C. to 180° C. and an outlet temperature of 89° C. to 94° C. The resulting fine powder of Paenibacillus sp. strain B containing 10% precipitated silica (w/w with respect to the total dry mass of the resulting dry product) was used as technical active ingredient (TGAI) in the following examples 4 to 6. In Table 4 this TGAI is referenced to as P.5.

[0736] Paenibacillus sp. strain B TGAI, referenced as P.5, was tank-mixed with various formulation additives diluted in tab water as listed in Table 4. The resulting spray slurry was tested against Alternaria solani on tomato, Botrytis cinerea on pepper and Phytophtera infenstans on tomato in greenhouse pot trials as described in detail in example 1.

TABLE-US-00006 TABLE 4 Antifungal activity of Paenibacillus sp. strain B TGAI combined with tank-mix additives at different concentrations (% w/w). Dose rate of Additive Efficacy Efficacy Efficacy Spray TAGI Additive in concen- Efficacy Efficacy Efficacy slurry/ P.5 % dilution tration ALTESO BOTRCI PHYTIN treatment w/w medium % w/w % % % 1 control None 0 0 0 2 0.056 None 89 89 92 3 0.056 Tensiofix ® BCZ 0.01 94 80 77 4 0.056 Wettol ® D1 0.01 11 2 2 5 0.056 Agnique ® SLES 370 0.01 74 83 75 6 0.056 Polyfon ® H 0.1 53 42 0 7 0.056 Wettol ® D3 0.1 15 0 2 8 0.056 Borresperse Na 0.1 53 66 47 9 0.056 Sokalan ® HP 50 0.1 83 97 79 10 0.056 Pluronic ® PE3100 0.1 83 95 85 11 0.056 Break-Thru ® SP133 0.1 90 95 77 12 0.111 Agnique ® SLES 370 0.03 90 95 84 13 0.111 Borresperse Na 0.2 86 70 68 14 0.111 Sokalan ® HP 50 0.2 98 95 99 15 0.111 Pluronic ® PE3100 0.2 99 89 98 16 0.111 Break-Thru ® SP133 0.2 99 99 100

[0737] Treatments with polyvinyl pyrrolidone, polyethylene—polypropylene glycol block copolymer, polyglycerol ester or with lauryl ether sulfate in the spray slurry performed much better than treatments containing other anionic wetting or dispersing agents (lauryl sulfate, naphthalene sulfonic acid co-condensate, lignosulfonates) usually used in dry and liquid crop protection formulations and well known to the skilled person.

Example 4: Greenhouse Screening for Compatible Additives with Paenibacillus sp. Strain B Powder in Tank-Mix

[0738] Paenibacillus sp. strain B TGAI from example 3, referenced as P.5, was tank-mixed at a concentration of 0.083% with formulation additives diluted in tab water at two different concentrations as listed in Table 5. The resulting spray slurry was tested against Phytophtora infestans on tomatoes in greenhouse pot trials as described in detail in example 1. Table 5 shows the test results in relation to TGAI P.5 diluted in water.

TABLE-US-00007 TABLE 5 Activity of Paenibacillus sp. strain B TGAI combined with various tank-mix additives against Phytophtora infestans. Additives providing improved efficacy are highlighted in bold. TGAI concentration is 0.083% w/w in all cases. Additive Efficacy Test Additive in concentration % PHYTIN No. dilution medium (w/w) % 1 none 0.000 65 1 Atlox ™ 4913 0.020 44 1 Atlox ™ 4913 0.150 77 1 Atlox ™ 4915 0.020 20 1 Atlox ™ 4915 0.150 48 1 Atlox ™ 4916 0.020 41 1 Atlox ™ 4916 0.150 87 1 Luvitec ® VA64 0.020 84 1 Luvitec ® VA64 0.150 88 1 Pluronic ® PE10500 0.020 77 1 Pluronic ® PE10500 0.150 94 1 Pluronic ® PE6400 0.020 65 1 Pluronic ® PE6400 0.150 97 1 Borresperse Ca 0.020 23 1 Borresperse Ca 0.150 41 2 none 0.000 50 2 Lutensol ® AT 11 0.020 31 2 Lutensol ® AT 11 0.150 50 2 Lutensol ® AT 80 0.020 50 2 Lutensol ® AT 80 0.150 81 2 Lutensol ® XL 70 0.020 68 2 Lutensol ® XL 70 0.150 68 2 Lutensol ® XL 140 0.020 47 2 Lutensol ® XL 140 0.150 65 2 Break-Thru ® EM O5 0.020 59 2 Break-Thru ® EM O5 0.150 68 2 Borresperse Na 0.020 33 2 Borresperse Na 0.150 29 3 none 0.000 26 3 Tween ® 20 0.020 22 3 Tween ® 20 0.150 44 3 Break-Thru ® SF 420 0.020 44 3 Break-Thru ® SF 420 0.150 24 3 Atlas ™ G-1086 0.020 59 3 Atlas ™ G-1086 0.150 28 3 Atlas ™ G-1096 0.020 59 3 Atlas ™ G-1096 0.150 48 3 Dispersogen PSL 100 0.020 24 3 Dispersogen PSL 100 0.150 17 3 Sokalan ® HP 20 0.020 31 3 Sokalan ® HP 20 0.150 56 4 none 0 45 4 Tensiofix ® BCZ 0.02 38 4 Tensiofix ® BCZ 0.15 13 4 Polyfon ® F 0.02 31 4 Polyfon ® F 0.15 16 4 Wettol ® D1 0.02 42 4 Wettol ® D1 0.15 5 4 Brij ®-23 0.02 36 4 Brij ®-23 0.15 56 4 Span ™ 80 0.02 56 4 Span ™ 80 0.15 5 4 Angique SLES 370 0.02 56 4 Angique SLES 370 0.15 73 4 Borresperse Ca 0.02 64 4 Borresperse Ca 0.15 24

[0739] The greenhouse results of Table 5 show that mainly nonionic additives positively influence the biological efficacy in a consistent way. Especially the additives from the chemical groups of polyethylene—polypropylene glycol block polymers (irrespective of hydrophobic-hydrophilic ratio and molecular weight), the group of alcohol ethoxylates and nonionic polymers or copolymers of vinylpyrrolidone provide a positive effect on biological efficacy. Also, Glyceryl oleate, Polyoxyethylene sorbitol hexaoleates and Polyoxyethylene (20) sorbitol monooleate, as well as ethoxylated polyethylene imine improved the efficacy on Phytophtora infestans in greenhouse pot trials when added to the spray slurry.

Example 5: Greenhouse Test for Compatible Additives with Paenibacillus sp. Strain C

[0740] Paenibacillus sp. strain C, a mutant of Paenibacillus sp. strain Lu17007, was grown in a complex medium to an OD of 25 to 50 and a spore content of 5×10.sup.7 CFU/ml to 5×10.sup.8 CFU/ml. The whole fermentation broth was processed by microfiltration and concentrated to create a pellet with 5.5% dry mass. The pellet fraction was mixed with finely ground precipitated silica and the suspension was spray dried under nitrogen atmosphere with an inlet temperature of 170° C. to 180° C. and an outlet temperature of 85° C. to 95° C. The resulting fine powder of Paenibacillus sp. strain C containing 10% precipitated silica (w/w with respect to the total dry mass of the resulting dry product) was used as technical active ingredient (TGAI) in the following example.

[0741] Paenibacillus sp. strain C TGAI was tank-mixed at a concentration of 0.029% with formulation additives diluted in tab water at two different concentrations as listed in table 6. These spray slurries were tested as treatment against Phytophtora infestans on tomatoes in greenhouse pot trials.

[0742] Also, for strain C, polyethylene—polypropylene glycol block polymers Pluronic® PE10500 and PE6200 significantly improved biological efficacy against Phytophtora infestans. For alkylpolyglucoside Agnique® PG 8107 and sodium lauryl ether sulfate Agnique® SLES the impact on efficacy depends on the concentration. While for the neutral alkylpolyglucoside Agnique® PG8107 the higher concentration was beneficial, the lower concentration was advantageous for the anionic surfactant sodium lauryl ether sulfate.

TABLE-US-00008 TABLE 6 Activity of Paenibacillus sp. strain C TGAI combined with various tank-mix additives against Phytophtora infestans. Additives providing improved efficacy are highlighted in bold. TGAI concentration is 0.029% w/w in all cases. Additive Efficacy Treatment Additive in concentration % PHYTIN No. dilution medium (w/w) % 1 none 0.000 65 2 Pluronic ® PE10500 0.020 92 3 Pluronic ® PE10500 0.150 99 4 Pluronic ® PE6200 0.020 92 5 Pluronic ® PE6200 0.150 98 6 Vanisperse ® CB 0.020 32 7 Vanisperse ® CB 0.150 9 8 Agnique ® PG 8107 0.020 53 9 Agnique ® PG 8107 0.150 92 10 Agnique ® SLES-370 0.020 87 11 Agnique ® SLES-370 0.150 68

Example 6: Wettable Powder Formulations of Paenibacillus sp. Strain C and their Greenhouse Activity

[0743] Paenibacillus sp. strain C was grown was grown in a complex medium to an OD of 25 to 50 and a spore content of 5×10.sup.7 CFU/ml to 5×10.sup.8 CFU/ml. The whole fermentation broth was processed by microfiltration and concentrated to create a pellet with 5.5% dry mass. The pellet fraction was mixed with finely ground precipitated silica and the suspension was spray dried under nitrogen atmosphere with an inlet temperature of 110° C. to 140° C. and an outlet temperature of 60° C. to 70° C. The resulting fine powder of Paenibacillus sp. strain C containing 10% precipitated silica (w/w with respect to the total dry mass of the resulting dry product) was used as technical active ingredient (TGAI) in the following example.

[0744] Further wettable powders were produced by adding formulation additives to the pellet fraction according to the recipes in table 7, followed by spray drying with the parameters mentioned above.

TABLE-US-00009 TABLE 7 Wettable powder formulations of Paenibacillus sp. strain C. Ingredient (% w/w) Function TGAI WP1 WP2 WP3 WP4 WP5 WP6 WP7 WP8 Bio dry mass 90 30 30 30 30 31 30 30 30 Sipernat ® Insoluble 10 30 10 10 10 10 28 10 310 filler Chalk Insoluble 30 (calcium filler carbonate) Ammonium Soluble 20 20 20 20 sulfate filler Magnesium Soluble 20 sulfate filler Potassium Soluble 12 phosphate filler monobasic Potassium Soluble 6 phosphate filler dibasic Pluronic ® Category 1 10 10 20 20 20 10 10 10 PE 10500 additive Pluronic ® Category 1 10 10 19 19 20 10 10 10 PE 6200 additive Agnique ® Category 2 1 1 1 1 1 SLES 370 additive Borresperse Category 3 39 Na additive Vanisperse ® Category 3 39 2 CB additive

[0745] Spray slurries produced from the wettable powder formulations as listed in table 7, containing 0.028% w/w to 0.087% w/w formulation were tested as treatment against Phytophtora infestans on tomatoes and Botrytis cinerea on pepper in greenhouse pot trials, as describe in detail in example 1. From the results listed in table 8, it is clearly visible that WP 1 to WP 5, containing additives of category 1, show superior biological efficacy compared to the TGAI at the same biomass content. On the other hand, WP 6 containing substantial amount of anionic sulfonated dispersing agent almost lost its efficacy, while WP 7 with a significantly lower amount of anionic sulfated dispersing agent is still inferior to WP 1 to WP 5.

TABLE-US-00010 TABLE 8 Activity of Paenibacillus sp. strain C wettable powder formulations against Phytophtora infestans and Botrytis cinerea. Efficacy Efficacy Treatment Formulation % w/w % w/w PHYTIN BOTRCI No No formulation biomass % % 1 TGAI 0.028 0.025 43 53 2 WP1 0.087 0.026 84 92 4 WP2 0.087 0.026 80 68 6 WP3 0.087 0.026 87 76 8 WP4 0.087 0.026 88 94 10 WP5 0.087 0.026 89 92 12 WP6 0.087 0.026 14 23 14 WP7 0.087 0.026 61 90 3 WP1 0.174 0.052 88 100 5 WP2 0.174 0.052 96 100 7 WP3 0.174 0.052 89 97 9 WP4 0.174 0.052 98 100 11 WP5 0.174 0.052 94 98 13 WP6 0.174 0.052 32 31 15 WP7 0.174 0.052 84 99

Example 7: Wettable Powder Formulations of Paenibacillus sp. Strain C and their Greenhouse Activity

[0746] Further wettable powders listed in table 9 were produced by adding formulation additives to Paenibacillus polymyxa C pellet fraction followed by spray-drying according to the procedure described in example 6.

TABLE-US-00011 TABLE 9 Wettable powder formulations of Paenibacillus sp. strain C. Ingredient (% w/w) Function WP9 WP10 WP11 TGAI Bio dry mass 30 30 30 90 Sipernat ® 310 Insoluble filler 17 30 23 10 Ammonium sulfate Soluble filler 20 20 20 Break Thru S 301 Wetting agent 2 2 Break Thru SP 133 Category 1 5 additive Pluronic ® Category 1 13 10 15 PE10500 additive Pluronic ® PE 6200 Category 1 13 10 additive Atlox ™ 4913 Category 2 5 5 additive

[0747] Spray slurries produced from dilutions of the wettable powder formulations of table 8 and table 9 in tap water, containing 0.017% w/w to 0.051% w/w formulation, were tested as treatment against Phytophtora infestans on tomatoes and Botrytis cinerea on pepper in greenhouse pot trials as described in example 1. The results are listed in table 10. It can be clearly seen that WP9, WP10 and WP11 containing the favorable formulation additives of category 1 show a much better performance than the TGAI, while formulations WP6 and WP8 containing category 3 additives in a substantial amount have significantly inferior biological efficacy.

TABLE-US-00012 TABLE 10 Activity of Paenibacillus sp. strain C wettable powder formulations against Alternaria solani, Phytophtora infestans and Botrytis cinerea. Efficacy Efficacy Efficacy Treatment Formulation % w/w % w/w ALTESO PHYTIN BOTRCI No No. Formulation biomass % % % 1 TGAI 0.017 0.015 41 55 61 2 TGAI 0.034 0.031 92 87 88 3 TGAI 0.051 0.046 99 99 100 4 WP10 0.051 0.015 73 85 92 5 WP10 0.102 0.031 84 100 92 6 WP6 0.037 0.011 0 0 3 7 WP6 0.074 0.022 0 82 21 8 WP8 0.037 0.011 0 10 21 9 WP8 0.074 0.022 36 17 72 10 WP9 0.051 0.015 63 87 80 11 WP9 0.102 0.031 90 93 96 12 WP9 0.153 0.046 95 100 98 13 WP11 0.051 0.015 75 79 90 14 WP11 0.102 0.031 92 99 98 15 WP11 0.153 0.046 98 99 97

Example 8 Suspension Concentrate Formulations of Paenibacillus sp. Strain C and their Greenhouse Activity

[0748] TGAI of Paenibacillus sp. strain C containing 10% precipitated silica was prepared as described in example 5. To prepare a suspension concentrate formulation, water and PEG 200 were mixed on a 1:1 w/w basis. After adding the other formulation ingredients listed in table 11, the TGAI was stirred in with low shear until a homogeneous mixture was achieved.

TABLE-US-00013 TABLE 11 Suspension concentrate formulations of Paenibacillus sp. strain C. Ingredient (g/L) Function SC1 SC2 SC3 SC4 SC5 TGAI 200 200 200 200 200 Water Diluent 450 415 390 390 390 PEG 200 Diluent 450 415 390 390 390 Pluronic ® Category 1 100 70 50 PE 10500 additive Pluronic ® Category 1 50 PE 6400 additive Pluronic ® Category 1 50 PE 6200 additive Break-Thru ® Category 1 30 SP 133 additive Atlox ™ 4913 Category 2 20 20 20 20 additive

[0749] Spray slurries were produced from the suspension concentrate formulations of table 11 by dilution in tap water, containing 0,107% w/w and 0,160% w/w of formulation. The fungicidal activity of the resulting spray suspensions was compared in greenhouse pot trials against Phytophthora infestans and Alternaria Solani, both in tomatoes, and against Botrytis cinerea in pepper, as previously described in detail in example 1. The results are listed in table 12. While the SC1 with no further formulation additives does not perform well in ALTESO and PHYTIN, SC2 to SC4 containing advantageous formulation additives of category 1 and 2, have significantly enhanced biological performance, also on BOTRCI.

TABLE-US-00014 TABLE 12 Activity of Paenibacillus sp. strain C suspension concentrate formulations against Alternaria solani, Phytophtora infestans and Botrytis cinerea. % w/w Efficacy Efficacy Efficacy Treatment Formula- formula- ALTESO BOTRCI PHYTIN No tion No tion % % % 1 SC1 0.107 70 59 29 2 SC1 0.160 38 90 5 3 SC2 0.107 88 78 49 4 SC2 0.160 88 99 76 5 SC3 0.107 90 77 61 6 SC3 0.160 91 98 85 7 SC4 0.107 92 73 83 8 SC4 0.160 96 90 80 9 SC5 0.107 90 77 73 10 SC5 0.160 89 85 81

Example 9: Non-Aqueous Suspension Concentrate Formulations of Paenibacillus sp. Strain C and their Greenhouse Activity

[0750] TGAI of Paenibacillus sp. strain C was prepared as described in example 5. Non-aqueous suspension concentrate formulations were prepared by mixing PEG 200 with the formulation additives listed in table 13, and as a last step stirring in 200 g/L TGAI with low shear until homogeneous, followed by one passage through a rotor-stator colloid mill IKA magic lab.

TABLE-US-00015 TABLE 13 Non-aqueous suspension concentrate formulations of Paenibacillus sp. strain C. Ingredient (g/L) Function SC6 SC7 SC8 SC9 TGAI 200 200 200 200 PEG 200 Diluent 900 820 760 780 Pluronic ® PE Category 1 50 6400 additive Agnique ® Category 1 50 PG8107 additive Atlox ™ 4913 Category 2 70 70 70 additive Aerosil ® 200 Anti-settling 10 20 agent

[0751] Spray slurries were produced from the suspension concentrate formulations of Table 13 by dilution in tap water, containing 0,107% w/w to 0,215% w/w of formulation. The fungicidal activity of the resulting spray suspensions was compared in greenhouse pot trials against Phytophthora infestans and Alternaria solani, both in tomato, and against Botrytis cinerea in pepper, as previously described in detail in Example 1. The results listed in Table 14 show that the non-aqueous suspension concentrate SC6 without additives has a good performance only at the highest application rate. Formulations with additives of category 1 and category 2, as SC7 to SC9, show further enhanced biological performance when compared to SC6, especially at the lower application rates.

TABLE-US-00016 TABLE 14 Activity of Paenibacillus sp. strain C non-aqueous suspension concentrate formulations against Alternaria solani, Phytophtora infestans and Botrytis cinerea. % w/w Efficacy Efficacy Efficacy Treatment Formula- Formula- ALTESO PHYTIN BOTRCI No tion No tion % % % 1 SC6 0.107 44 36 69 2 SC6 0.161 60 61 67 3 SC6 0.215 78 82 76 4 SC7 0.107 70 50 58 5 SC7 0.161 87 72 81 6 SC7 0.215 93 77 90 7 SC8 0.107 58 56 71 8 SC8 0.161 82 73 75 9 SC8 0.215 90 76 91 10 SC9 0.107 53 38 77 11 SC9 0.161 82 56 85 12 SC9 0.215 84 83 95

Example 10: Non-Aqueous Suspension Concentrate Formulations with Various Liquid Media

[0752] TGAI of Paenibacillus sp. strain C was prepared as described in example 5. Non-aqueous suspension concentrate formulations were prepared by mixing PEG 200 (SC10), triethyl citrate (SC11) or hexylene glycol (SC12), with the formulation additives listed in table 15, and as a last step stirring in 170 g/kg TGAI with low shear until homogeneous, followed by one passage through a rotor-stator colloid mill IKA magic lab.

[0753] Formulation samples were put into incubation chambers at 40° C. and reevaluated after 8 and 16 weeks. The results, also shown in table 15, prove that all three formulations display sufficient stability at accelerated storage conditions with high temperatures.

TABLE-US-00017 TABLE 15 Non-aqueous suspension concentrate formulations of Paenibacillus sp. strain C. Ingredient (g/kg) Function SC10 SC11 SC12 TGAI 170 170 170 PEG 200 Diluent 710 Triethyl citrate Diluent 710 Hexylene glycol Diluent 710 Pluronic ® PE 6400 Category 1 additive 20 20 20 Atlox ™ 4913 Category 2 additive 85 85 85 Aerosil ® 200 Anti-settling agent 10 10 10 Struktol ® J673 A Antifoam 5 5 5 Initial physical parameters Viable spores cfu/ml 2.8E+09 2.9E+09 3.6E+09 Particle size d50/d90 μm  9.5/21.4  9.4/22.1  9.7/22.1 Viscosity, 100/s mPas 224 178 160 pH, 1% dilution 6.2 6.2 6.3 After 8 weeks 40° C. Viable spores cfu/ml 3.4E+09 4.0E+09 4.6E+09 Particle size d50/d90 μm 10.4/20.3 13.6/74.1 11.4/22.8 Viscosity, 100/s mPas 256 195 238 pH, 1% dilution 5.8 4.7 5.5 After 16 weeks 40° C. Viable spores cfu/ml 3.0E+09 2.3E+09 1.4E+09 Particle size d50/d90 μm 16.1/36.7 14.3/65.4 12.0/27.0 Viscosity, 100/s mPas 333 198 306 pH, 1% dilution 6.4 4.3 6 Serum/sediment traces/none traces/none traces/none Redispersibility good good good

[0754] After 16 weeks of storage at 40° C., spray slurries were produced from the suspension concentrate formulations of Table 16 by dilution in tap water, containing 0.095% w/w to 0,196% w/w of formulation. The fungicidal activity of the resulting spray suspensions was compared in greenhouse pot trials against Phytophthora infestans and Alternaria solani; both in tomato, as previously described in detail in Example 1. The results listed in Table 16 show that the non-aqueous suspension concentrates with different dilution media SC10, SC11 and SC12 all display show good biological performance starting at the intermediate dose rate. SC10 and SC11 have a weaker performance than SC12 at lower dose rate.

TABLE-US-00018 TABLE 16 Activity of Paenibacillus sp. strain C non-aqueous suspension concentrate formulations against Alternaria solani and Phytophtora infestans Efficacy Efficacy Treatment Formulation % w/w ALTESO PHYTIN No No Formulation % % 1 SC10 0.095 43 51 2 SC10 0.143 80 92 3 SC10 0.190 89 82 4 SC11 0.096 56 65 5 SC11 0.144 83 86 6 SC11 0.192 92 87 7 SC12 0.098 78 76 8 SC12 0.147 87 94 9 SC12 0.196 92 83