COSMETIC COMPOSITION COMPRISING CAPSULES

Abstract

A cosmetic composition comprising a plurality of capsules and a cosmetic base is provided.

Claims

1. Cosmetic composition comprising a plurality of capsules and a cosmetic base, wherein the capsules comprise a core encapsulated in a shell layer, said core comprising a liquid containing a cosmetic active compound, wherein said liquid comprises an emulsion of a first liquid and a second liquid, said first and second liquid being substantially immiscible with one another, wherein said cosmetic active compound is present in said first liquid and the core of said emulsion is surrounded by said shell layer, wherein the cosmetic active compound is selected from the group consisting of a vitamin and/or a salt and/or an ester thereof, a natural colorant, an energetic molecule, a cofactor, an antioxidant, an anti-acne agent, a whitening agent, an enzyme, a self-tanning reagent, a plant stem cell, a peptide, a polypeptide, a protein, a polysaccharide, a cooling agent, a warming or tingling agent, a liposoluble compound, a powder, a pigment, resveratrol, azelaic acid, ellagic acid and/or a salt and/or an ester thereof, fumaric acid and/or a salt and/or an ester thereof, rutin, ferulic acid, escin, sericoside, asiaticoside, madecassoside, salicylic acid, and mixtures thereof, and wherein the cosmetic base is selected from the group consisting of a cream, a lotion, a gel, an oil, a shampoo base, a shower gel, a hair conditioner, and a serum.

2. Cosmetic composition according to claim 1, wherein the cosmetic active compound is selected from the group consisting of: a vitamin or vitamin derivative selected from the group consisting of ascorbic acid, an ascorbic acid salt or ester (e.g. ascorbyl palmitate or ascorbyl acetate), retinol, a retinyl ester (e.g. retinyl palmitate), thiamine, riboflavin, nicotinic acid, a nicotinic acid salt or ester, pantothenic acid, a pantothenic acid salt or ester, pyridoxine, biotin, folic acid, a folic acid salt or ester, cyanocobalamin, lipoic acid, a lipoic acid salt or ester, and niacinamide; in particular ascorbic acid and/or a salt thereof; a natural colorant selected from the group consisting of an anthocyanin (e.g. cyanidin, peonidin, malvidin, delphinidin, petunidin, pelargonidin), curcumin, chlorophyll, lycopene, beta-carotene, capsanthin, capsorubin, and spirulina; an energetic molecule or cofactor selected from the group consisting of adenosine triphosphate (ATP), adenosine diphosphate (ADP), nicotinamide adenine dinucleotide (NAD+ or NADH), nicotinamide adenine dinucleotide phosphate (NADP+ or NADPH), coenzyme Q10, superoxide dismutase (SOD), and glutathione; an antioxidant selected from the group consisting of a polyphenol, thiol-based component, a sulphite and derivatives thereof, tocopherol, carnosic acid, a tocotrienol, and a flavonoid; an anti-acne and/or whitening agent selected from the group consisting of benzoyl peroxide, glycyrrhizin acid, and a glycyrrhizin acid derivative; an enzyme selected from the group consisting of a lipase, a protease, an esterase, papain, and bromelain; a spider silk protein; a polysaccharide selected from the group consisting of hyaluronic acid and/or a salt and/or a derivative thereof (e.g. sodium hyaluronate, hyaluronic acid acetate or a cationized hyaluronic acid), xanthan gum, and rhizobium gum; a cooling agent selected from the group consisting of menthol and a menthol derivative (e.g. a menthyl carboxamide); a warming or tingling agent selected from the group consisting of alpha hydroxy sanshool, capsaicin, spilanthol, ginger oil, and black pepper oil; a liposoluble compound selected from the group consisting of jojoba oil, cranberry oil, rosephip oil, argan oil, kendi oil, grapeseed oil, bilberry seed oil, coffee oil, apricot kernel oil, buriti oil, chia seed oil, camelina oil, tsubaki oil, tea tree oil, karanja oil, moringa oil, tea seed oil, ungurahui oil, nymaplung oil, manila oil, bisabolol, and canabidiol; a powder selected from the group consisting of zinc oxide, titanium dioxide, charcoal, and bamboo powder; and mixtures thereof.

3. Cosmetic composition according to claim 1, wherein the cosmetic base is a cream comprising an emulsifier, e.g. Ceteth-20 and/or Cetyl Alcohol and/or Glyceryl Stearate and/or PEG-75 Stearate and/or Steareth-20; fatty acids, e.g. medium-chain fatty acids (such as medium-chain triglycerides), stearic acid, palmitic acid, Cetearyl Wheat Straw Glycosides and/or Cetearyl Alcohol, sucrose ester (such as sucrose palmitate, sucrose distearate, and/or sucrose tristearate); a preservative, e.g. phenoxyethanol and/or paraben and/or a paraben blend; and water; and optionally further comprising one or more of a humectant, e.g. glycerin; a fragrance; a pigment; and a color.

4. Cosmetic composition according to claim 1, wherein the cosmetic base is a gel comprising a thickener, e.g. xanthan gum; a preservative, e.g. phenoxyethanol and/or paraben and/or a paraben blend; and water; and optionally further comprising one or more of a humectant, e.g. glycerin, a fragrance, a pigment; and a color.

5. Cosmetic composition according to claim 1, wherein the cosmetic base is a jellified oil, comprising an emollient, e.g. coco-glycerides and/or octyldodecanol and/or C12-15 alkyl benzoate; a thickener, e.g. silica and/or cellulose and/or a cellulose derivative, such as ethyl cellulose, dextrin palmitate, dextrin myristate, dextrin palmitate ethylhexanoate, bentonite; and optionally further comprising one or more of xanthan gum; acrylate copolymers (e.g. carbomers); a fragrance; a pigment; and a color.

6. Cosmetic composition according to claim 1, wherein the cosmetic base is a shampoo base comprising a surfactant, e.g. sodium laureth sulfate and/or cocamidopropyl betaine; a preservative, e.g. phenoxyethanol; and water; and optionally further comprising one or more of a conditioner, e.g. hydroxypropyl guar and/or hydroxypropyl guar hydroxypropyltrimonium chloride; a pH modulator, e.g. an acid, such as citric acid, or a base; a viscosity controller and/or bulking agent, e.g. sodium chloride or another salt; a fragrance; a pigment; and a color.

7. Cosmetic composition according to claim 1, wherein the cosmetic base is a shampoo base comprising a surfactant, e.g. coco-glucoside and/or decyl-glucoside and/or a quillaja saponaria wood extract; an emollient, e.g. glyceryl oleate; a preservative, e.g. sodium benzoate and/or potassium sorbate; and water; and optionally further comprising one or more of a conditioner, e.g. xanthan gum; a pH modulator, e.g. an acid, such as lactic acid, or a base; a fragrance; a pigment; and a color.

8. Cosmetic composition according to claim 1, wherein the cosmetic base is selected from the group consisting of: TABLE-US-00014 Base Composition Cream 5% of a mixture of Ceteth-20, Cetyl Alcohol, Glyceryl Stearate, PEG-75 Stearate and Steareth-20 (e.g. Emulium Delta); 12% medium-chain triglycerides (e.g. Mygliol 812 N); 0.4% phenoxyethanol and/or paraben and/or a paraben blend (e.g. Phenonip XB); 1% glycerin; and 80.6% water Gel 1% xanthan gum; 3% glycerin; 0.5% phenoxyethanol (e.g. Phenoxetol); and 80.6% water Jellified 25.5% C12-15 alkyl benzoate (e.g. DUB B1215); 20% coco- oil glycerides (e.g. Myritol 331); 43.5% octyldodecanol (e.g. Eutanol G); 3% ethyl cellulose (e.g. Ethocel Std 100 Premium; and 7% silica (e.g. Aerosil 200) Shampoo 28.93% sodium laureth sulfate (e.g. SLES Texapon NSO 27% in water); 12% cocamidopropyl betaine (e.g. Dehyton Kcos 30% in water); 0.6% phenoxyethanol; 0.2% hydroxypropyl guar and/or hydroxypropyl guar hydroxypropyltrimonium chloride (e.g. Jaguar C162); 0.07% citric acid; 0.5% sodium chloride; 0.2% fragrance; and 56.5% water Sulfate- 1% xanthan gum (e.g. XGF FEDCS-PC); 0.2% coco- free glucoside (e.g. Plantacare 818 UP); 20% decyl-glucoside shampoo (e.g. Oramix NS 10); 7% coco-glucoside/glyceryl oleate (e.g. Lamesoft PO 65); 0.2% quillaja saponaria wood extract (e.g. Sapnov vegan); 0.3% sodium benzoate; 0.2% potassium sorbate; 2% lactic acid (e.g. Purac HS 90); and 62.1% water

9. Cosmetic composition according to claim 1, wherein the shell layer comprises calcium alginate.

10. Cosmetic composition according to claim 1, comprising about 0.1 to about 10% of capsules, more preferably about 0.5 to about 5% of capsules, e.g. about 1% of capsules.

11. Cosmetic composition according to claim 1, wherein the capsules have an average diameter of about 70 to about 5000 μm, more preferably of about 1000 to about 3000 μm, most preferably about 1500 to about 2500 μm.

12. Cosmetic composition according to claim 1, wherein the cosmetic active compound constitutes about 20-50 wt % of the capsules.

13. Cosmetic composition according to claim 1, wherein the capsules are visible to the eye.

14. Cosmetic composition according to claim 1, wherein the shell layer is configured to break or rupture under a mechanical load.

15. Cosmetic composition according to claim 1, wherein the shell layer comprises a colorant and/or pigment selected from the group consisting of an anthocyanin (e.g. cyanidin, peonidin, malvidin, delphinidin, petunidin, pelargonidin), curcumin, chlorophyll, lycopene, beta-carotene, capsanthin, capsorubin, spirulina, zinc oxide, titanium dioxide, iron oxide, charcoal, and mixtures thereof.

16. Cosmetic composition according to claim 1, additionally comprising one or more further cosmetic actives.

Description

[0175] The invention is described in further detail with reference to a number of exemplary embodiments and accompanying drawings:

[0176] FIG. 1 shows a specific example of a capsule according to the invention; and

[0177] FIG. 2 shows an embodiment of a formulation according to the invention.

[0178] It is noted that the figures are drawn purely schematically and not necessarily to a same scale. In particular, certain dimensions may have been exaggerated to a more or lesser extent to aid the clarity of any features. Similar parts are generally indicated by a same reference numeral throughout the figures.

[0179] The capsules 10 that are shown in FIG. 1 are manufactured using a method according to the invention and resemble the protective nature that is provided by the invention to a vulnerable active compound that would otherwise be prone to oxidation or other degradation when exposed to ambient air, for instance. The active compound comprises for example ascorbic acid, being readily soluble in water. By dissolving micronized powder, for instance having a modal particle size of below around 75-100 micron, a large quantity of for instance between 20 and 25 wt % of ascorbic acid may be brought in an aqueous hydrophilic phase 11. The hydrophilic phase 11 is brought in a suspension with a hydrophobic continuous phase 12, for instance containing a plant oil like sunflower oil. Solid nano-clay particles of laponite clay may be added to the mixture to act as a Pickering stabilizer to stabilize the emulsion, counteracting coalescence of the aqueous phase droplets 11 and phase separation. The emulsion comprised of small dispersed hydrophilic droplets 11 surrounded by a hydrophobic continuous phase 12 is encapsulated within a hydrophilic solid shell layer 13, for instance created by a solidified cross-linked hydrophilic calcium alginate network. The capsules 10 typically enclose a volume of less than one milliliter, thereby forming a micro-capsule containing said suspension comprising said first liquid 11 and said second liquid 12, protecting said active compound in said first liquid.

[0180] The formulation of FIG. 2 comprises a plurality of micro-capsules 10 as shown in FIG. 1, which contain an appropriate active compound. The capsules are distributed in a suitable fluid material 20 like a cream, lotion, gel, serum, cleanser, soap, shampoo, oil or clay to provide a cosmetic composition that expresses the desired properties of the active compound.

[0181] The present invention is further illustrated by means of the following non-limiting examples:

EXAMPLE 1: PRESERVATION OF ASCORBIC ACID (AA) VIA ENCAPSULATION IN OIL-FILLED CALCIUM-ALGINATE CAPSULES

[0182] Ascorbic acid was encapsulated in oil-filled alginate capsules. Specifically:

[0183] Creation of a Preserved AA Laden Sample.

[0184] 50% (w/v) AA sodium salt (sodium L-ascorbate) was dissolved in water, which was then emulsified in sunflower oil. A stable surfactant-free water-in-oil (w/o) emulsion was generated by shaking and ultrasonically treating degassed water and oil solutions.

[0185] The AA-laden w/o emulsion was kept at 70° C. while it was jetted with a flow rate of 13 ml/min from the center nozzle of a coaxial nozzle assembly (OD=1.6 mm and ID=0.41 mm) as disclosed in the aforementioned co-pending European patent application EP 3 436 188 A1, the subject matter of which in respect to the capsule generation is herewith incorporated by reference.

[0186] Concurrently, a 0.5% (w/v) sodium alginate in water solution (WAKO, 1% ˜80-120 cP) of room temperature was jetted from the outer nozzle of the same coaxial nozzle assembly at a flow rate of 55 ml/min, resulting in a compound jet consisting of AA-laden w/o emulsion encapsulated by a sodium alginate solution. The coaxial nozzle was modulated, using a vibrating element at a frequency of approximately 150 Hz, which caused the controlled breakup of the compound jet into a stream of substantially mono-disperse, i.e. substantially uniformly sized, compound droplets with typically a coefficient of variation in size or diameter of less than 10%.

[0187] Via a so called ‘in-air microfluidics’ method, as described in the aforementioned co-pending application (EP 3 436 188), the droplet stream was combined with an intact, i.e. uninterrupted jet, of a 0.2 M calcium in water solution, resulting in the formation of compound hydrogel capsules consisting of a calcium-alginate shell layer filled with a core of AA-laden w/o emulsion. These hydrogel capsules were stored in water and incubated for 6 weeks at 40° C.

[0188] (ii) Creation of a Non-Preserved AA Laden Reference Sample.

[0189] For comparison purposes, a non-preserved AA control sample was created by dissolving 0.5% (w/v) AA in water, resulting in the same final concentration as that of the preserved sample. Also this non-preserved AA in water solution was incubated for 6 weeks at 40° C.

[0190] (iii) Comparison

[0191] Comparing a color change of the preserved sample to that of the similarly incubated (6 weeks at 40° C.) reference solution showed a brown coloring of the reference solution that is typical for the oxidation product of AA, while the preserved sample showed no significant coloring. This revealed that the encapsulation of AA in oil-filled capsules, according to the invention, reduces coloring, indicating suppression or even prevention of AA degradation.

EXAMPLE 2: BOTH PHYSICAL ENCAPSULATION AND THE PRESENCE OF CALCIUM

[0192] To investigate the mechanism of AA preservation via encapsulation in calcium-alginate shells, three conditions were compared: [0193] (a) AA preserved as described in example 1, under (i); [0194] (b) Non-preserved control: same as (a), but using a 0.2 M sodium chloride solution instead of a 0.2 M calcium chloride solution, which will prevent the formation of gelled calcium-alginate capsules and thus the encapsulation of the AA-laden w/o emulsion; [0195] (c) Non-preserved control: same as (a), but the 0.2 M calcium chloride jet was not impacted with the compound droplets prior to collection in the collector bath, which prevents the in-flight formation of stable calcium-alginate capsules and, thus, the encapsulation of the AA-laden w/o emulsion.

[0196] The concentration of AA in all conditions was at least 5% (w/v) in the w/o emulsions and at least 0.5% (w/v) in the final sample volumes.

[0197] Comparison of the color change after incubation at 40° C. for 4 weeks showed no significant coloring of the preserved sample (a), comprising AA-laden hydrogel capsules having a calcium cross-linked alginate shell layer, while sample (b) showed significant browning after 4 weeks and sample (c) showed slight coloring. This revealed that the presence of divalent calcium ions has a preserving effect on AA.

EXAMPLE 3: OIL-FILLED CAPSULES USING PICKERING EMULSIFIERS

[0198] Fumed silica nanoparticles post-treated with dimethyl-dichlorosilane (DDS) (Evonik, Aerosil R972) were added to the core of the capsules by dispersing 2% (w/v) of nano-particles in the oil phase. The oil containing hydrophobized silica particles was then processed following the same method as described in example 1, except that a supersaturated AA-solution containing 100% (w/v) was used. In this example, calcium carbonate (CaCO.sub.3) was added to the dispersed AA-laden phase before emulsification to load the capsules with excess CaCO.sub.3 that will aid in maintaining a stable calcium-alginate shell over time.

[0199] The thus created AA-laden silica-oil filled calcium-alginate capsules had a final AA concentration in the w/o emulsion (i.e. the core compound) of at least 10% (w/v), a final CaCO.sub.3 concentration in the w/o emulsion of 2% (w/v) and a final hydrophobized silica concentration in the w/o emulsion of 2% (w/v). The capsules were washed two times with demineralized water and incubated in demineralized water at 40° C. for 23 days.

[0200] After 23 days, the samples showed no significant coloring, revealing an effective preservation of both the AA active compound in the dispersed phase as well as of the w/o emulsion itself and the surrounding calcium-alginate shell layer.

EXAMPLE 4: OIL-FILLED CAPSULES USING PICKERING EMULSIFIERS IN CORE AND ADDITIVES IN SHELL

[0201] Fumed silica nano-particles were post-treated with dimethyl-dichlorosilane (DDS) (Evonik, Aerosil R972) and added to the core of the capsules by dispersing 4% (w/v) of nano-particles in an oil phase. The oil containing these hydrophobized silica particles was processed following the same method as described in example 1 together with an aqueous solution of ascorbic acid to form an emulsion, except that a saturated L-ascorbic acid fine powder containing 40% (w/v) was used for the solution.

[0202] Furthermore, 0.5% laponite XL21XR nano-clay was added to the 0.5% alginate phase in the shell during the encapsulation. This created AA-laden silica-oil filled calcium-alginate/laponite capsules, having a final AA concentration in the w/o emulsion (i.e. the core compound) of at least 10% (w/v), and a final hydrophobized silica concentration in the w/o emulsion of 4% (w/v).

[0203] The capsules were washed two times with demineralized water and incubated in demineralized water, 0.2 M CaCl.sub.2+10 wt % ethanol solution, clear hand gel, shampoo, and body lotion, respectively, at 40° C. for 4 weeks. After 4 weeks, the samples showed no significant coloring compared to a similar sample containing the same emulsion without encapsulation and L-ascorbic acid bulk solution in water. This revealed an effective preservation of both the AA active compound in the dispersed phase as well as of the w/o emulsion itself by the surrounding calcium-alginate shell layer.

EXAMPLE 5: AGAR REINFORCED ENCAPSULATED CAPSULES

[0204] Similar capsules were prepared using the same method as described in example 4, but additionally using 0.5 wt % agar solution for forming the shell composition. This resulted in ascorbic acid containing emulsion capsules within a 1% laponite XL21XR/0.5% alginate/0.5% agar solid shell layer. These capsules were stored for 4 weeks in water, shampoo, body lotion and 0.2 M CaCl.sub.2+10% EtOH, respectively, and were found to show hardly any coloring compared to the bare emulsion (i.e. unencapsulated ascorbic acid emulsion) in the same base.

EXAMPLE 6: LAYER-BY-LAYER COATING OF THE SOLID SHELL

[0205] Capsules were prepared in a similar way to that of example 4, but with coating by a layer using electrostatically interaction of layer-by-layer method (LBL). The alginate shell was coated by a positively charged biopolymer, such as chitosan, to enhance the barrier property and stability of L-ascorbic acid. Chitosan was applied as a polycationic polymer to ionically crosslink the alginate shell.

[0206] Initially, a stable 5 wt % chitosan stock solution was prepared in aid of 1% (v/v) hydrochloric acid (HCl) while stirring for several hours at 50° C. Afterwards, the 5 wt % chitosan solution was neutralized by adjusting the pH to about 6-7 by adding sodium hydroxide (NaOH) solution. Then, the neutralized solution was diluted to obtain a 0.5 wt % chitosan solution to be used in the coating process.

[0207] Vitamin C encapsulated capsules in an alginate shell were coated with a chitosan/alginate bilayer. First, the capsules were rinsed with water and then incubated in a 0.5 wt % neutralized chitosan solution while stirring slowly for 15 min. The incubated beads were filtered and rinsed with water, then incubated and stirred slowly in 0.5 wt % of alginate solution for another 15 min. To avoid the aggregation of capsules during static incubation, slow movement (stirring) was applied while incubating the beads. To achieve a denser coating, a salt solution (NaCl solution) was applied to coat the same capsules by ionic crosslinking of chitosan/alginate. The purpose of using salts was to ensure attaining a stable thickness by alternating deposition. A 0.8 M NaCl solution was applied in all incubation and rinsing steps.

EXAMPLE 7: IMPROVED STABILIZATION USING SOLIDIFIED SOLID WAX PARTICLES

[0208] Similar capsules were prepared according to the method as described in example 4, but instead of fumed silica nano-particles, 2 wt % micronized carnauba wax (Microcare 350, Micro Powder Inc.) was added to the oil phase (i.e., the second liquid).

[0209] Alternatively, a sample was prepared containing an oil phase with 4 wt % hydrophobized fumed silica, as well as 2 wt % micronized carnauba wax.

[0210] The capsules were then incubated for 0.5, 1, 2, 5, 10, and 20 min at 40, 60, 75, or 90° C. 5 min incubation at 90° C. appeared optimal for melting the micronized wax particles in the capsules in order to render the suspension more stable after the wax had again solidified in the oily phase.

[0211] The capsules were tested for ascorbic acid stability by incubating them in water, shampoo, body lotion and 0.2M CaCl.sub.2+10% EtOH, at 40° C. After this treatment, the capsules showed hardly any yellow coloring as compared to the same particles that were mechanically disrupted at the start of the stability test, indicating an improved chemical stability (i.e., preservation) of the encapsulated ascorbic acid within capsules containing a Pickering and wax stabilized emulsion of the dispersed ascorbic acid aqueous phase.

EXAMPLE 8: ELECTROSTATICALLY LOADING THE FIRST LIQUID

[0212] An aqueous phase from the below list was added to 6 wt % Aerosil R972 to act as a Pickering stabilizer in sunflower oil. Three homogenization cycles using T25 Ultra-Turrax with S25N-25F dispersing element were applied to form a Pickering emulsion: 1 cycle of 2 min at 10,000 rpm and 2 cycles of 1 min at 10,000 rpm. In between the cycles, the emulsion was mixed manually to ensure a total incorporation of the phases. [0213] 1. Water [0214] 2. Water+1 wt % NaCl [0215] 3. Water+HCl (pH 2) [0216] 4. Water+20 wt % hyaluronic acid [0217] 5. Water+20 wt % hyaluronic acid+1 wt % NaCl [0218] 6. Water+2 wt % carrageenan [0219] 7. Water+2 wt % acacia gum [0220] 8. Water+2 wt % acacia gum+1 wt % NaCl [0221] 9. Water+1 wt % chitosan

[0222] The resulting emulsion of samples 4-7 appeared to be significantly more stable than the other samples. This supports the assumption that the water-in-oil Pickering emulsion based on negatively charged Aerosil R972 as the Pickering agent is further stabilized by the addition of polyanionic polymers or (negatively charged) glycosaminoglycans, notably by hyaluronic acid, carrageenan or acacia gum, to the aqueous phase.

EXAMPLE 9: STABILITY TESTING OF CAPSULES IN COSMETIC FORMULATIONS

[0223] Ten different types of capsules in six different cosmetic bases were subjected to stability testing. Samples were kept at 4° C., 20° C., and 40° C.

Capsules

[0224] The following ten types of capsules were tested:

TABLE-US-00002 # Capsules 1 0.5% calcium-alginate matrix capsules, suspended in 0.2M CaCl.sub.2 + 10% EtOH 2 0.5% calcium-alginate matrix capsules with iron oxide yellow pigment, suspended in 0.2M CaCl.sub.2 + 10% EtOH 3 0.5% calcium-alginate + 0.5% agar matrix capsules with iron oxide yellow pigment, suspended in 0.2M CaCl.sub.2 + 20% EtOH 4 0.5% calcium-alginate matrix capsules with iron oxide yellow pigment + CaCO.sub.3 microparticles, suspended in 0.2M CaCl.sub.2 + 10% EtOH 5 0.5% calcium-alginate matrix capsules with iron oxide yellow pigment + Laponite XL21 XR, suspended in 0.2M CaCl.sub.2 +10% EtOH 6 0.5% calcium-alginate + 0.5% agar matrix capsules with iron oxide yellow pigment + Laponite XL21 XR, suspended in 0.2M CaCl.sub.2 + 20% EtOH 7 0.5% calcium-alginate matrix capsules with chlorophyll in a sunflower oil core, suspended in 0.2M CaCl.sub.2 + 10% EtOH 8 0.5% calcium-alginate with 0.5% Laponite XL21 XR + 0.5% agar-based capsules with chlorophyll in a sunflower oil core, suspended in 0.2M CaCl.sub.2 + 20% EtOH 9 0.5% calcium-alginate with 0.5% agar-based capsules with chlorophyll in a sunflower oil core, suspended in 0.2M CaCl.sub.2 + 20% EtOH 10 0.5% calcium-alginate + Laponite XL21 XR based capsules with an emulsion-filled core containing 40% L-ascorbic acid in water + 4% Aerosil ® R972 in sunflower oil, suspended in 0.2M CaCl.sub.2 + 20% EtOH

Cosmetic Compositions

[0225] The above ten capsule types 1-10 were tested in the following six cosmetic formulations, the compositions of which are described in the six tables below (concentrations indicated in % weight by weight):

[0226] A. Classical Shampoo

TABLE-US-00003 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 SLES Texapon 28.93 28.93 28.93 28.93 28.93 28.93 28.93 28.93 28.93 28.93 NSO 27% Dehyton Kcos 30% 12 12 12 12 12 12 12 12 12 12 Phenoxyethanol 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 Fragrance 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Water 36.5 36.5 36.5 36.5 36.5 36.5 36.5 36.5 36.5 36.5 Jaguar C162 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Water 20 20 20 20 20 20 20 20 20 20 Citric acid 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 NaCl powder qs 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 visco 5000 cps Capsule Type #1 1 Capsule Type #2 1 Capsule Type #3 1 Capsule Type #4 1 Capsule Type #5 1 Capsule Type #6 1 Capsule Type #7 1 Capsule Type #8 1 Capsule Type #9 1 Capsule Type #10 1

[0227] B. Sulfate-Free Shampoo

TABLE-US-00004 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 XGF FEDCS-PC 1 1 1 1 1 1 1 1 1 1 (=xanthan gum) Water 59.5 59.5 59.5 59.5 59.5 59.5 59.5 59.5 59.5 59.5 Plantacare 818 UP 20 20 20 20 20 20 20 20 20 20 (=coco-glucoside) Oramix NS 10 7 7 7 7 7 7 7 7 7 7 (=decyl-glucoside) Lamesoft PO 65 = 6 6 6 6 6 6 6 6 6 6 (coco-glucoside, glyceryl oleate) Sapnov vegan 2 2 2 2 2 2 2 2 2 2 Water 1 1 1 1 1 1 1 1 1 1 Sodium benzoate 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Potassium sorbate 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Purac HS 90 2 2 2 2 2 2 2 2 2 2 (=lactic acid) Capsule Type #1 1 Capsule Type #2 1 Capsule Type #3 1 Capsule Type #4 1 Capsule Type #5 1 Capsule Type #6 1 Capsule Type #7 1 Capsule Type #8 1 Capsule Type #9 1 Capsule Type #10 1

[0228] C. Cream

TABLE-US-00005 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 Emulium Delta 5 5 5 5 5 5 5 5 5 5 Miglyol 12 12 12 12 12 12 12 12 12 12 Water 80.6 80.6 80.6 80.6 80.6 80.6 80.6 80.6 80.6 80.6 Phenonip XB 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Glycerin 1 1 1 1 1 1 1 1 1 1 Capsule Type #1 1 Capsule Type #2 1 Capsule Type #3 1 Capsule Type #4 1 Capsule Type #5 1 Capsule Type #6 1 Capsule Type #7 1 Capsule Type #8 1 Capsule Type #9 1 Capsule Type #10 1

[0229] D. Gel

TABLE-US-00006 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 Xanthan gum 1 1 1 1 1 1 1 1 1 1 Glycerin 3 3 3 3 3 3 3 3 3 3 Water 94.5 94.5 94.5 94.5 94.5 94.5 94.5 94.5 94.5 94.5 Phenoxethol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Capsule Type #1 1 Capsule Type #2 1 Capsule Type #3 1 Capsule Type #4 1 Capsule Type #5 1 Capsule Type #6 1 Capsule Type #7 1 Capsule Type #8 1 Capsule Type #9 1 Capsule Type #10 1

[0230] E. Jellified Oil

TABLE-US-00007 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 DUB B1215 = C12-15 25.5 25.5 25.5 25.5 25.5 25.5 25.5 25.5 25.5 25.5 Alkyl benzoate Myritol 331 = Cocoglycerides 20 20 20 20 20 20 20 20 20 20 Eutanol G = Octyldodecanol 43.5 43.5 43.5 43.5 43.5 43.5 43.5 43.5 43.5 43.5 Ethocel Std 100 3 3 3 3 3 3 3 3 3 3 Premium = Ethylcellulose Aerosil 200 = Silica 7 7 7 7 7 7 7 7 7 7 Capsule Type #1 1 Capsule Type #2 1 Capsule Type #3 1 Capsule Type #4 1 Capsule Type #5 1 Capsule Type #6 1 Capsule Type #7 1 Capsule Type #8 1 Capsule Type #9 1 Capsule Type #10 1

[0231] F. Hand Sanitizer

TABLE-US-00008 F1 F2 F3 F4 F5 F6 F F8 F9 F10 Carbopol Ultrez 20 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Water 20.25 20.25 20.25 20.25 20.25 20.25 20.25 20.25 20.25 20.25 Triethanolamine sq pH 6.5 EtOH 96% 75 75 75 75 75 75 75 75 75 75 Phenoxethol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Fresh Hydration 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 68 EAE08472/00 Vegetal glycerin 3 3 3 3 3 3 3 3 3 3 rapeseed Capsule Type #1 1 Capsule Type #2 1 Capsule Type #3 1 Capsule Type #4 1 Capsule Type #5 1 Capsule Type #6 1 Capsule Type #7 1 Capsule Type #8 1 Capsule Type #9 1 Capsule Type #10 1

Procedures

[0232] The cosmetic compositions were prepared by first preparing the respective base and then adding the capsules at room temperature under very gentle stirring with a spatula.

[0233] The viscosity of the cosmetic compositions was determined using a Brookfield DVIII ultra spindle E or cylinder 3 and speed 12.

[0234] The pH was measured at 20° C.

Results

[0235] The results of the stability testing are compiled in the following tables (compositions that were unstable at the beginning of the test (D0) were not tested further and are listed below the respective tables):

A. Classical Shampoo

[0236]

TABLE-US-00009 A2 A3 A5 A8 A9 A10 D0 Viscosity 1380 1270 1150 1510 280 1350 [mPa .Math. s] pH 5.1 5.12 5.15 4.98 5.04 5.17 Aspect Sedimentation, but Sedimentation, Capsules at the surface Sedimentation, capsules intact but capsules but capsules intact intact 28 d at Aspect OK Smaller OK OK OK OK 4° C. capsules Smell OK OK OK OK OK PK 28 d at Aspect OK Smaller OK Capsules Capsules Beginning 4° C. capsules less green less green Lysis Smell OK OK OK OK OK OK 28 d at Aspect OK Smaller OK Capsule Capsule Pale yellow 4° C. capsules broken and broken and and lysis of sedimentation green at capsules the surface Smell OK OK OK OK OK oxidated Unstable at D0: A1, A4, A6, A7

B. Sulfate-Free Shampoo

[0237]

TABLE-US-00010 B1 B2 B3 B4 B5 D0 Viscosity 6540 6830 6490 6870 6840 [mPa .Math. s] pH 5.42 5.38 5.35 5.35 5.36 Aspect Homogenous capsules in suspension 28 d at Aspect OK OK OK OK OK 4° C. Smell OK OK OK OK OK 28 d at Aspect 2-3 capsules OK OK 2-3 capsules OK 4° C. broken broken Smell OK OK OK OK OK 28 d at Aspect Beginning of OK Beginning of Broken OK 4° C. sedimentation sedimentation capsules and and smaller and smaller sedimentation capsules capsules Smell OK OK OK OK OK B6 B7 B8 B9 B10 D0 Viscosity 7180 5730 6750 6150 6540 [mPa .Math. s] pH 5.36 5.36 5.35 5.34 5.33 Aspect Homogenous capsules in suspension 28 d at Aspect OK Capsule Small halo Small halo OK 4° C. deformation around around capsules capsules Smell OK OK OK OK OK 28 d at Aspect OK Capsule Small halo Small halo OK 4° C. deformation around around and less capsules, capsules, green about ½ about ½ capsules capsules broken broken Smell OK OK OK OK OK 28 d at Aspect Clear capsules Smaller Small halo Small halo Yellowing 4° C. on top and broken around around of capsules white capsules capsules capsules, capsules, and base at the bottom and release about ½ about ½ of dye capsules capsules broken broken Smell OK OK OK OK OK

C. Cream

[0238]

TABLE-US-00011 C1 C2 C3 C4 C5 D0 Viscosity 12300 15300 13300 13400 13400 [mPa .Math. s] pH 4.98 5.04 5.20 5.52 5.66 Aspect Homogenous capsules in suspension 28 d at Aspect OK OK OK OK OK 4° C. Smell OK OK OK OK OK 28 d at Aspect OK OK OK OK OK 4° C. Smell OK OK OK OK OK 28 d at Aspect OK OK OK OK OK 4° C. Smell OK OK OK OK OK C6 C7 C8 C9 C10 D0 Viscosity 10000 16000 18300 10100 9200 [mPa .Math. s] pH 4.80 5.00 4.68 5.11 4.87 Aspect Homogenous capsules in suspension 28 d at Aspect OK OK OK OK OK 4° C. Smell OK OK OK OK OK 28 d at Aspect OK OK OK OK OK 4° C. Smell OK OK OK OK OK 28 d at Aspect Smaller OK OK OK Capsules 4° C. capsules yellowing Smell OK OK OK OK OK

D. Gel

[0239]

TABLE-US-00012 D1 D2 D3 D4 D5 D0 Viscosity 4250 3960 4120 4790 4510 [mPa .Math. s] pH 5.89 5.97 6.25 7.99 6.22 Aspect Homogenous capsules in suspension 28 d at Aspect Capsule Light capsule Capsule Light capsule 4° C. agglomerates agglomerates agglomerates agglomerates Smell OK OK OK OK OK 28 d at Aspect Capsule Light capsule Capsule Light capsule 4° C. agglomerates agglomerates agglomerates agglomerates Smell OK OK OK OK OK 28 d at Aspect Capsule Light capsule Capsule Light capsule 4° C. agglomerates agglomerates agglomerates agglomerates Smell OK OK OK OK OK D6 D7 D8 D9 D10 D0 Viscosity 3040 3150 3810 3710 3850 [mPa .Math. s] pH 6.29 6.27 6.03 6.22 6.37 Aspect Homogenous capsules in suspension 28 d at Aspect Capsules Shell ½ shell OK 4° C. broken disappearance disappearance Smell OK OK OK OK OK 28 d at Aspect Smaller Shell ½ shell OK 4° C. capsules disappearance disappearance Smell OK OK OK OK OK 28 d at Aspect OK Shell ½ shell Pale 4° C. disappearance disappearance yellow capsules Smell OK OK OK OK OK

E. Jellified Oil

[0240]

TABLE-US-00013 E1 E2 E3 E4 E5 E6 D0 Viscosity 18000 18600 1880 18400 18040 20100 [mPa .Math. s] Aspect Limpid and homogenous capsules in suspension 28 d at Aspect OK OK OK OK OK OK 4° C. Smell OK OK OK OK OK OK 28 d at Aspect OK OK Smaller capsules 1 smaller OK 4° C. at the surface capsule and with a beginning beginning of of sedimentation sedimentation Smell OK OK OK OK OK OK 28 d at Aspect Smaller capsules at the surface with a beginning of 4° C. sedimentation, hazy aspect at the bottom Smell OK OK OK OK OK OK E7 E8 E9 E10 D0 Viscosity 18100 18500 19000 18500 [mPa .Math. s] Aspect Limpid and homogenous capsules in suspension 28 d at Aspect OK OK OK OK 4° C. Smell OK OK OK OK 28 d at Aspect OK OK OK OK 4° C. Smell OK OK OK OK 28 d at Aspect Less green Less green Pale green Yellow smaller 4° C. at the at the capsules with capsules with surface, surface a beginning of a beginning of hazy at sedimentation, sedimentation, the bottom hazy aspect at hazy aspect at the bottom the bottom Smell OK OK OK OK

F. Hand Sanitizer

[0241] The tested capsules were incompatible with the tested base.

Application to Skin

[0242] Compositions were applied to the skin (on the hand) after 1 month at 20° C. The following was observed: [0243] Compositions A5 and A10 left no residue. [0244] The capsules of composition A2 broke upon application, but there were some residues. [0245] The capsules of composition A3 broke in the formula when trying to pick one capsule. [0246] Compositions B1-B10 left no residue. [0247] Composition B2 was slower to penetrate the skin than B1 and B3-B10. [0248] The capsules in compositions C1-C4 and C6 did not break upon application to the skin. [0249] The capsules in compositions C5, C9 and C10 did break upon application, but left a residue. [0250] The capsules in compositions C7 and C8 did break upon application. [0251] For compositions D1-D3, D6, D7, D9 and D10, there was no residue. [0252] Compositions D4, D5, and D8 left a light residue. [0253] The capsules in compositions E1-E6 did not break upon application to the skin. [0254] The capsules in compositions E7-E10 did break upon application, but left a residue.

CONCLUSIONS

[0255] The following observations were made: [0256] All capsules were compatible with and stable in the tested cream base. [0257] The hand sanitizer base tested was not compatible with the tested capsules. [0258] No release of the yellow pigment was observed, except for the shampoo base A. [0259] Chlorophyll was released in all bases, except for the cream base. [0260] The yellow pigment was stable in the gel base. [0261] Chlorophyll-containing capsules lost their shell in the gel base. [0262] For yellow pigment in shampoo base A, only compositions A2, A3, and A4 were stable. [0263] In all bases tested, capsules 2 (+agar) and 5 (Laponite XL21+agar) were the most stable capsules. [0264] Yellowing was observed for more or less all capsules containing vitamin C in all bases.

[0265] Although the invention has been described herein before with reference to merely a limited number of explanatory embodiments, it should be understood that the invention is by no means limited to those examples. On the contrary, many more variations and embodiments are feasible to a skilled person within the framework of the present invention without requiring him or her to exercise any inventive skill. Particularly, further components other than the first liquid may also contain one or more active compounds, notably also the hydrophobic second liquid and/or the shell layer.

[0266] The first liquid may be emulsified directly with the second liquid or may find itself in an emulsion with one or more further liquid to be jointly emulsified or mixed with the second liquid. Also, the second liquid may itself consist of an emulsion with a further liquid. Each fluid may be used as a carrier for one or more specific active compounds or ingredients.