Surgical implant material for assisted repair of muscle mechanics and method of preparing the same

Abstract

The present invention provides a surgical implant material for assisted repair of muscle mechanics and a method of preparing the same. The surgical implant material for assisted repair of muscle mechanics comprises a collagen compound within a net-like bacterial cellulose base material. A bacterial cellulose base material is placed into solution of collagen, treated via vortex shaking, dried at room temperature; and then immersed in an aqueous solution of an aldehyde compound under vacuum to react for 10 to 30 minutes, thereby producing the surgical implant material for assisted repair of muscle mechanics. The surgical implant material of the present invention can effectively improve the biocompability, and maintain the flexibility, smoothness and fitness of the base material to reduce the damage to surrounding tissues, thereby reducing the bleeding and inflammatory response. Meanwhile, the processing conditions of the preparation method is more reasonable and convenient to control, and more suitable for industrial scale-up.

Claims

1. A method of preparing a surgical implant material for assisted repair of gynecological pelvic floor muscles, comprising: placing a net-like bacterial cellulose base material into a solution of collagen at a certain concentration, treating in a manner of vortex shaking for 30 seconds to 10 minutes, drying at room temperature; placing the net-like bacterial cellulose base material into an aqueous solution of glutaraldehyde at a concentration of 0.5-10 wt % at 30-50° C., wherein the aqueous solution comprises a water-soluble inorganic aluminum salt, the aqueous solution is disturbed for immersion for 50-70 minutes; and immersing the net-like bacterial cellulose base material in a vacuum environment having a vacuum degree of 0.06-0.5 Mpa at 60-120° C. for 10-30 min, wherein the net-like bacterial cellulose base material undergoes a reaction to produce the surgical implant material for assisted repair of gynecological pelvic floor muscles, wherein the weight percentage of nitrogen atoms is 5-15% in the surgical implant material for assisted repair of gynecological pelvic floor muscles, and a surface of the material has a root-mean-square (RMS) roughness of 40-50 nm in the surgical implant material for assisted repair of gynecological pelvic floor muscles.

2. The method of preparing the surgical implant material for assisted repair of gynecological pelvic floor muscles of claim 1, wherein the water-soluble inorganic aluminum salt is present in the aqueous solution at a concentration of 0.1-3 wt %.

Description

DETAILED DESCRIPTION OF THE INVENTION

Embodiment 1

(1) A method of preparing the aforesaid surgical implant material for assisted repair of muscle mechanics was carried out by the steps of:

(2) Step 1: dissolving collagen into acetic acid to give a 3 wt % solution of collagen in acetic acid;

(3) Step 2; placing a net-like bacterial cellulose base material which was purified by removal of endotoxin in accordance with CN201810180455.4 into the solution in acetic acid prepared in Step 1, treating the mixture by means of vortex shaking with a vortex-genie 2 type vortex shaker adjusted to Gear 8 for 10 minutes, and hanging to dry at room temperature;

(4) Step 3: placing the base material obtained in Step 2 into a 3 wt % aqueous solution of glutaraldehyde, adding 0.02% Al.sub.2(SO4).sub.3 into the solution, and immersing at 37° C. in a shaker at 110 rpm for 60 minutes; and

(5) Step 4: Reacting the material immersed in Step 3 at 90° C. in a vacuum drying oven at a vacuum degree of 0.08 Mpa for 30 min, to produce the surgical implant material for assisted repair of muscle mechanics.

Embodiment 2

(6) The material of Example 1, a bacterial cellulose base material (Comparative Example 1) and a bacterial cellulose surgical implant material coated with collagen on the surface and prepared in accordance with the prior art (Comparative Example 2) were detected for the content of nitrogen atoms and the surface roughness of materials, respectively. The results are shown in Table 1.

(7) TABLE-US-00001 TABLE 1 Percent of RMS Surface Sample Nitrogen Atoms Roughness, nm Comparative Example 1 0 27.5 Comparative Example 2 1.7 75.6 Example 1 5.56 45.7

(8) The aforesaid measurements show that as compared with the material of Comparative Example 2, the material of Example 1 enables the bacterial cellulose to comprise more biocompatibility-improving collagen (the percent of nitrogen element reflects the content of collagen because nitrogen is contained only in collagen). Meanwhile, as compared with the Comparative Example 2, the material of Example 1 has substantially improved surface roughness, and can substantially maintain the softness, smoothness, fitness of the base material to reduce the damage to surrounding tissues caused by friction, thereby reducing bleeding and inflammatory responses.

Embodiment 3

(9) A surgical implant material for assisted repair of muscle mechanics was prepared by the same method of Example 1 except that:

(10) 1. The time of vortex shaking was 30 seconds;

(11) 2. The concentration of the aqueous solution of glutaraldehyde was 10%, and the time of immersion at 48° C. with magnetic stirring was 50 minutes;

(12) 3. The base material immersed in Step 3 was reacted at 60° C. in a vacuum environment of 0.5 Mpa for 10 min.

(13) After detection, the above-prepared surgical implant material comprises 14.89% of nitrogen atom. After surface roughness detection, the material have a RMS surface roughness of 42.6 nm.

Embodiment 4

(14) A surgical implant material for assisted repair of muscle mechanics was prepared by the same method of Example 1 except that:

(15) 1. a solution of collagen in hydrochloric acid was utilized;

(16) 2. the time of vortex shaking was 8 minutes;

(17) 3. the concentration of the aqueous solution of glutaraldehyde was 0.5%, and the time of immersion was 70 minutes;

(18) 4. the base material immersed in Step 3 was reacted at 120° C. in a vacuum environment of 0.2 Mpa for 15 min.

(19) After detection, the above-prepared surgical implant material comprises 8.78% of nitrogen atom. After surface roughness detection, the material has a RMS surface roughness of 50.4 nm.