Methods for Treating Respiratory Diseases Characterized by Mucus Hypersecretion

Abstract

Abstract: The invention therefore provides methods of treating a respiratory disease characterized by mucus hyper-secretion comprising administering to a human patient in need of such treatment a gamma secretase inhibitor (GSI), wherein the administration of a GSI is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in said patient’s lungs. In some embodiments, the methods of the invention are effective in treating a respiratory disease selected from the group consisting of cystic fibrosis, chronic obstructive pulmonary disease, primary ciliary dyskinesis, chronic bronchitis, asthma, idiopathic and secondary bronchiectasis, bronchiolitis obliterans, idiopathic pulmonary fibrosis and other fibrotic lung disorders, respiratory infection including exacerbations in chronic respiratory disorders, and mucus accumulation in response to acute infection. Methods of the invention further include methods of treating cystic fibrosis wherein a GSI is administered to a patient being administered or in need of a CFTR modulator, wherein the mucus in such patient’s lungs is reduced or mucus accumulation in such patient’s lungs is inhibited.

Claims

1. A method of treating a respiratory disease characterized by mucus hyper-secretion comprising: administering a low dose of a GSI to a human patient in need of such treatment; and wherein the mucus in such patient’s lungs is reduced or mucus accumulation in such patient’s lungs is substantially ameliorated or prevented upon administration of the GSI.

2. The method of claim 1, wherein the low dose is an effective amount to treat the respiratory disease characterized by mucus hyper-secretion and is a lower dose as compared to a dose of the GSI suitable for administering to a patient suffering from a neurodegenerative disorder, an oncology disorder, or a respiratory disease not characterized by mucus hyper-secretion.

3. The method of claim 2 wherein the low dose of a GSI yields a peak plasma level in the submicromolar range.

4. The method of claim 1 wherein the respiratory disease is selected from the group consisting of cystic fibrosis, chronic obstructive pulmonary disease, primary ciliary dyskinesis, chronic bronchitis, asthma, idiopathic and secondary bronchiectasis, bronchiolitis obliterans, idiopathic pulmonary fibrosis and other fibrotic lung disorders and respiratory infection, including exacerbations in chronic respiratory disorders and mucus accumulation in response to acute infection.

5. The method of any of the preceding claims wherein the GSI is selected from the group consisting of semagacestat, avagacestat, GS-1, DBZ, L-685,458, BMS-906024, crenigascestat, MRK 560, nirogacestat, RO-4929097, MK-0752, itanapraced, LY-3056480, fosciclopirox, tarenflurbil, and begacestat.

6. The method of claim 5 wherein said GSI is selected from the group consisting of semagacestat, nirogacestat, MK-0752, RO-492907, or crenigacestat.

7. The method of claim 6 wherein the GSI is semagacestat.

8. The method of claim 6 wherein the GSI is MK-0752.

9. The method of claim 6 wherein the GSI is nirogacestat.

10. The method of claim 6 wherein the GSI is RO-492907.

11. The method of claim 6 wherein the GSI is crenigacestat.

12. The method of any of the preceding claims wherein said administration of GSI is by oral administration.

13. The method of any of the preceding claims wherein the respiratory disease is cystic fibrosis or chronic obstructive pulmonary disease.

14. A method of treating a respiratory disease characterized by mucus hyper-secretion comprising: systemically administering to a human patient in need of such treatment a therapeutically effective amount of semagacestat, wherein said patient’s semagacestat plasma concentration at steady state following multiple dose administration comprises an AUC less than 1220 ng•hr/mL, and wherein the administration of semagacestat is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs.

15. The method of claim 14 wherein the respiratory disease is selected from the group consisting of cystic fibrosis, chronic obstructive pulmonary disease, primary ciliary dyskinesis, chronic bronchitis, asthma, idiopathic and secondary bronchiectasis, bronchiolitis obliterans, idiopathic pulmonary fibrosis and other fibrotic lung disorders and respiratory infection, including exacerbations in chronic respiratory disorders and mucus accumulation in response to acute infection.

16. The method of claim 15 wherein the respiratory disease is cystic fibrosis or chronic obstructive pulmonary disease.

17. The method of claim 14, claim 15 or claim 16 wherein the semagacestat is administered in an amount of from about 0.1 mg to about 50 mg daily.

18. The method of claim 17 wherein about 0.5 mg to about 40 mg of semagacestat is administered daily.

19. The method of claim 18 wherein about 0.5 mg to about 40 mg of semagacestat is administered daily.

20. The method of claim 19 wherein about 0.5 mg to about 30 mg of semagacestat is administered daily.

21. The method of claim 20 wherein about 0.5 mg to about 20 mg of semagacestat is administered daily.

22. A method of treating cystic fibrosis comprising: administering an effective amount of a GSI to a human patient being administered or in need of a CFTR modulator, wherein the mucus in such patient’s lungs is reduced or mucus accumulation in such patient’s lungs is inhibited.

23. The method of claim 17 wherein the GSI is selected from the group consisting of semagacestat, avagacestat, GS-1, DBZ, L-685,458, BMS-906024, crenigascestat, MRK 560, nirogacestat, RO-4929097, MK-0752, itanapraced, LY-3056480, fosciclopirox, tarenflurbil, and begacestat.

24. The method of claim 18 wherein the GSI is selected from the group consisting of semagacestat, nirogacestat, MK-0752, RO-492907, or crenigacestat.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0022] FIG. 1 shows dose response data of semagacestat in primary human nasal epithelial cells (HNECs) compared to untreated cells and DAPT positive control. MCCs are labeled in green (acetylated tubulin) and cell junctions are labeled in red (ECAD). The percentage of MCCs increases from its baseline at 15.625 nM semagacestat to its maximum at around 125 nM. Toxicity is observed at micromolar doses.

[0023] FIG. 2 shows the ratio of MCCs to total luminal cells in HNECs treated with DAPT and various doses of semagacestat.

[0024] FIG. 3 shows method of scoring the ratio of MCCs to non-ciliated cells in airway epithelia in mice treated systemically in vivo by intraperitoneal (IP) dosing of semagacestat. Airways from lung sections of similar size were labeled for all nuclei (red; DAPI) and MCC cell fate (green; FoxJ1 and blue; acetylated tubulin). Cells were scored and the percentage of MCCs determined prior to unblinding treatment condition. Otherwise wildtype mice carried FoxJ1::GFP to facilitate scoring of MCCs.

[0025] FIG. 4 shows the body weight at days 9, 24 and 30 with daily systemic (IP) administration of semagacestat and vehicle control.

[0026] FIG. 5 shows the ratio of MCCs to total cells at day 7 following a three-day treatment with DAPT and low and high doses of semagacestat, with vehicle control.

[0027] FIG. 6 shows the ratio of MCCs to total cells at day 31 following a three-week treatment with semagacestat, with vehicle control.

[0028] FIG. 7 shows the effect of GSI treatment during proliferation (prior to differentiation) and during differentiation of HNECs.

[0029] FIG. 8 shows the quantitation of MCCs per total luminal cells of the data of FIG. 7.

[0030] FIG. 9 shows the effects of GSI treatment duration on mature (ALI+30d) HNECs, treated with DAPT and semagacestat for one (ALI+30 to +37d) or two weeks (ALI+30 to +44d).

[0031] FIG. 10 shows the quantitation of MCCs per total luminal cells of the data of FIG. 9.

[0032] FIG. 11 shows the results of HNECs treated with DAPT and semagacestat during differentiation only (ALI+0 to +21d) from either the apical or basal surface.

[0033] FIG. 12 shows the quantitation of MCCs per total luminal cells of the data of FIG. 11.

[0034] FIG. 13 shows the effect of semagacestat and DAPT treatment in an epithelial culture model of chronic airway inflammation. HNEC cultures were treated with IL-13 from ALI+7 to 14 to induce inflammation. DAPT and semagacestat increase the percentage of MCCs in controls (left). IL-13 treatment increases the percentage of mucin positive secretory cells and decreases the percentage of MCCs. Subsequent DAPT and semagacestat treatment rescues cell composition, increasing the percentage of MCCs and decreasing the percentage of mucin positive secretory cells.

[0035] FIG. 14 shows the quantitation of MCCs per total luminal cells of the data of FIG. 13.

[0036] FIG. 15 shows representative Ussing chamber tracings of cultures treated with ETI, semagacestat, or both.

[0037] FIG. 16 shows representative tracings of Ussing-chamber short circuit currents (Isc) following treatment with semagacestat in wild-type and CF cells.

[0038] FIG. 17 shows Ussing-chamber Isc responses following treatment with semagacestat in wild-type and CF cells. Two wild-type control samples (WT) and two CF patient samples (CF1; a rare allelic combination and CF2; a F508Δ homozygote) were studied. CFTR current activity were assessed by CFTR inhibitor response and were found to be as great as or greater than vehicle control currents in both wild-type controls and in CF samples. Values are normalized to the baseline current.

[0039] FIG. 18 shows that under in vitro treatment in combination with the CFTR modulator lumacaftor, semagacestat decreased mucus production in human CF samples with different CFTR mutations.

[0040] FIG. 19 shows the effect of treatment with semagacestat, lumacaftor and combinations on human CF samples with different CFTR mutations. Semagacestat is effective at increasing the percentage of MCCs in the presence of Lumacaftor, and the combination may be more effective for some donors than when either is applied alone.

[0041] FIG. 20 shows the quantitation of MCCs per total luminal cell data for healthy patient and CF donor 1 of FIG. 19.

[0042] FIG. 21 shows the effects on primary healthy and cystic fibrosis airway epithelial cells treated with semagacestat (LY45139) during differentiation only (ALI+0 to +21d).

[0043] FIG. 22 shows SEM of healthy and CF primary human airway epithelial cultures, showing that multiciliated cells formed under DAPT treatment are indistinguishable from those in untreated healthy cultures.

[0044] FIG. 23 shows the result of DAPT treatment in mature cystic fibrosis HNEC cultures, demonstrating that GSI treatment induces the formation of additional multiciliated cells in mature cystic fibrosis cultures, while untreated cultures do not differentiate any more multiciliated cells.

[0045] FIG. 24 shows the results of HNECs treated during differentiation (ALI+0 to +21d) with DAPT and high and low concentrations of the GSIs LY45139, PF-03084014, RO-4929097 and MK-0752.

[0046] FIG. 25 shows the quantitation of MCCs per total luminal cells of the data shown in FIG. 24.

[0047] FIG. 26 shows measurements of CFTR short-circuit current activity measured in Ussing chambers in cultures from CF patients treated with LY45139, Elexcaftor/Tezacaftor/Ivacaftor (or “ETI”), or both.

[0048] FIG. 27 shows measurements of CFTR current activity measured in Ussing chambers in cultures from CF patients treated with MK04752, ETI, or both.

[0049] FIG. 28 shows measurements of CFTR current activity measured in Ussing chambers in cultures from CF patients treated with MK04752, ETI, or combinations with reduced doses of Elexacaftor (E component of the ETI modulator combination).

[0050] FIG. 29 shows the results of the GSI DAPT treatment on ionocyte formation in HNECs treated with DAPT during differentiation only (ALI+0 to +21 d).

[0051] FIG. 30 shows the effect of varying concentrations of GSI MK-0752 and Elexacaftor of ciliary beat frequency (CBF).

[0052] FIG. 31 shows ciliary beat frequency (CBF) and cilium length of HNEs treated with DAPT versus untreated controls.

[0053] FIG. 32 shows the airway surface liquid (ASL) reabsorption characteristic of CF HNEC cultures treated with ETI, semagacestat, or both.

[0054] FIG. 33 shows images taken from a high-speed video recorded microscopic images of latex bead movement as a reflection of mucus transport by the ciliated surface of cell cultures. Cultures were of HNECs from two CF donors (F508del homozygotes) under treatment with vehicle control, ETI, Semagacestat (LY) or both treatments combined.

[0055] FIG. 34 shows the calculated bead movement of the cultures shown in FIG. 33.

DETAILED DESCRIPTION OF THE INVENTION

Definitions

[0056] As used herein, a “disease characterized by mucus hypersecretion” means a disease wherein at least one pathology of the disease is due to presence of mucus at an epithelial surface in excess of the amount present under normal conditions. Included are diseases in which excess mucus is located in small airway passageways in which it is not normally present, and may be due to excess goblet cell production, hypertrophy of mucus glands, decreased MCCs, or other inadequate mucociliary clearance.

[0057] As used herein, an “effective amount” or “therapeutically effective amount” refers to an amount of the compound of the present disclosure that is effective to achieve a desired therapeutic result such as, for example decreasing goblet cell production and/or increasing production of multiciliated cells, thereby improving mucociliary clearance. In the context of the present invention, a desired therapeutic result includes reducing mucus production in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. While the doses mentioned in the present disclosure are guidelines, an attending physician may adjust the dose according to the specific needs of the patient, including for example, severity of the disease, size and physical condition.

[0058] “Gamma secretase inhibitor(s)” or “GSI(s)” means a molecule capable of inhibiting or modulating the gamma secretase enzyme, and thereby inhibiting Notch signaling. Examples include DAPT (N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester), semagacestat, avagacestat ((2R)-2-[[(4-Chlorophenyl)sulfonyl][[2-fluoro-4-(1,2,4-oxadiazol-3-yl)phenyl]methyl]amino]-5,5,5-trifluoropentanamide) (commercially available from www.toeris.com), DBZ (N-[(1S)-2-[[(7S)-6,7-Dihydro-5-methyl-6-oxo-5H-dibenz[b,d]azepin-7-yl]lamino]-1-methyl-2-oxoethyl]-3,5-difluorobenzeneacetamide) (commercially available from www.toeris.com), L-685,458 ((5S)-(tert-Butoxycarbonylamino)-6-phenyl-(4R)-hydroxy-(2R)-benzylhexanoyl)-L-leucy-L-phenylalaninamide) (commercially available from www.toeris.com), GS-1 (aka L-685458) (CAS Registry number 292632-98-5; WO0177144); BMS-906024 (bis(fluoroalkyl)-1,4-benzodiazepinone; CAS Registry Number 1401066-79-2), Crenigascestat (aka LY3039478) (Massard et al., “First-in-human study of LY3039478, a Notch signaling inhibitor in advanced or metastatic cancer,” J Clin Oncol (2015) 33(15_suppl):2533), MRK 560 (N-[cis-4-[(4-Chlorophenyl)sulfonyl]-4-(2,5-difluorophenyl)cyclohexyl]-1,1,1-trifluoromethanesulfonamide) (commercially available from www.tocris.com), nirogacestat (aka PF-03084014)((S)-2-((S)-5,7-difluoro-1,2,3,4-tetrahydronaphthalen-3-ylamino)-N-(1-(2-m-ethyl-1-(neopentylamino)propan-2-yl)-1H-imidazol-4-yl)pentanamide; the CAS Registry Number is 865773-15-5; (commercially available from www.adoq.com)), RO-4929097 (RO4929097 refers to 2,2-dimethyl-N-((S)-6-oxo-6,7-dihydro-5H-dibenzo[b,diazepin-7-yl)-N′-(2,-2,3,3,3-pentafluoro-propyl)-malonamide. The CAS Registry Number is 847925-91-1) (commercially available from www.adooq.com), MK-0752 (CAS No. 471905-41-6 (www.medchemexpress.com)); itanapraced (CAS No. 749269-83-8 (www.medchemexpress.com)); LY-3056480 (Samarajeewa, Anshula & Jacques, Bonnie & Dabdoub, Alain. (2019). Therapeutic Potential of Wnt and Notch Signaling and Epigenetic Regulation in Mammalian Sensory Hair Cell Regeneration. Molecular Therapy. 27. 10.101/j.ymthe.2019.03.017); fosciclopirox (available as a disodium heptahydrate) (Patel, M.R., et al., Safety, dose tolerance, pharmacokinetics, and pharmacodynamics of fosciclopirox (CPX-POM) in patients with advanced solid tumors. Journal of Clinical Oncology (2020) 38:6 suppl 518); tarenflurbil (CAS No. 51543-40-9; (2R)-2-(3-fluoro-4-phenylphenyl)propanoic acid); EVP-0962 (Rogers, K., et al., (2012). Modulation of γ-secretase by EVP-0015962 reduces amyloid deposition and behavioral deficits in Tg2576 mice. Molecular Neurodegeneration. 7. 61. 10.1186/1750-1326-7-61.; NIC5-15 ; E-2212 ; GSI-1 ; NGP-555 ; PF-0664867); begacestat (aka GSI-953) (5-Chloro-N-[(1S)-3,3,3-trifluoro-1-(hydroxymethyl)-2-(trifluoromethyl)propyl]-2-thiophenesulfonamide) (www.tocris.com); GSI-136 (5-chloro-N-[(2S)-3-ethyl-1-hydroxypentan-2-yl]thiophene-2-sulfonamide) (https://pubchem.ncbi.nlm.nih.gov/compound/gsi-136); and BMS-708163 (Gillman, KW et al, Discovery and Evaluation of BMS-708163, a Potent, Selective and Orally Bioavailable Gamma-Secretase Inhibitor. ACS Med. Chem. Lett. (2010) 1(3)120-124). See also, Sekioka, R. et al., Discovery of N-ethylpyridine-2-carboxamide derivatives as a novel scaffold for orally active gamma secretase modulators. Bioorg. & Med. Chem., (2020) 28(1): 115132. “Carboxamide-based GSI” means a GSI having a carboxamide group, and includes molecules formed by carboxamide substitution as well as derivatives of known carboxamide-based GSIs such as DAPT. Examples include DAPT (N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester), semagacestat, avagacestat ((2R)-2-[[(4-Chlorophenyl)sulfonyl][[2-fluro-4-(1,2,4-oxadiazol-3-yl)phenyl]methyl]amino]-,5,5-trifluoropentanamide) (commercially available from www.toeris.com), DBZ (N-[(1S)-2-[[(7S)-6,7-Dihydro-5-methyl-6-oxo-5H-dibenz[b,d]azepin-7-yl]amino]-1-methyl-2-oxoethyl]-3,5-difluorobenzeneacetamide) (commercially available from www.toeris.com), L-685.458 ((5S)-(tert-Butoxycarbonylamino)-6-phenyl-(4R)-hydroxy-(2R)-benzylhexanoyl)-L-leucy-L-phenylalaninamide) (commercially available from www.toeris.com), BMS-906024 (bis(fluoroalkyl)-1,4-benzodiazepinone; CAS Registry Number 1401066-79-2), Crenigascestat (aka LY3039478) (Massard et al., “First-in-human study of LY3039478, a Notch signaling inhibitor in advanced or metastatic cancer,” J Clin Oncol (2015) 33(15_suppl):2533), MRK 560 (N-[cis-4-[(4-Chlorophenyl)sulfonyl]-4-(2,5-difluorophenyl)cyclohexyl]-1,1,1 -trifluoromethanesulfonamide) (commercially available from www.toeris.com), nirogacestat (aka PF-03084014)((S)-2-((S)-5,7-difluoro-1,2,3,4-tetrahydronaphthalen-3-ylamino)-N-(1-(2-m- ethyl-1-(neopentylamino)propan-2-yl)-1H-imidazol-4-yl)pentanamide; the CAS Registry Number is 865773-15-5; (commercially available from www.adooq.com)), RO-4929097 (RO4929097 refers to 2,2-dimethyl-N--((S)-6-oxo-6,7-dihydro-5H-dibenzo[b,diazepin-7-yl)-N′-(2,-2,3,3,3-pentafluoro-propyl)-malonamide. The CAS Registry Number is 847925-91-1) (commercially available from www.adooq.com) and BMS-708163 (Gillman, KW et al, Discovery and Evaluation of BMS-708163, a Potent, Selective and Orally Bioavailable Gamma-Secretase Inhibitor. ACS Med. Chem. Lett. (2010) 1(3)120-124). See also, Sekioka, R. et al., Discovery of N-ethylpyridine-2-carboxamide derivatives as a novel scaffold for orally active gamma secretase modulators. Bioorg. & Med. Chem., (2020) 28(1): 115132. GSIs include any salt form, polymorph, hydrate, analog, or pro-drug that retains gamma secretase inhibiting or modulating activity.

[0059] “Treat,” “treatment,” “prevent,” “prevention,” “inhibit” and corresponding terms include therapeutic treatments, prophylactic treatments, and ones that reduce the risk that a subject will develop a disorder or risk factor. Treatment does not require complete curing of disorder or condition, and includes the reduction in severity, reduction in symptoms, reduction of other risk factors associated with the condition and /or disease modifying effects such as slowing the progression of the disease.

[0060] Before the present invention is described in greater detail, it is to be understood that this invention is not limited to particular embodiments described, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present invention will be limited only by the appended claims.

[0061] Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges and are also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the invention.

[0062] Certain ranges are presented herein with numerical values being preceded by the term “about.” The term “about” is used herein to provide literal support for the exact number that it precedes, as well as a number that is near to or approximately the number that the term precedes. In determining whether a number is near to or approximately a specifically recited number, the near or approximating unrecited number may be a number which, in the context in which it is presented, provides the substantial equivalent of the specifically recited number.

[0063] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention, representative illustrative methods and materials are now described.

[0064] All publications and patents cited in this specification are herein incorporated by reference as if each individual publication or patent were specifically and individually indicated to be incorporated by reference and are incorporated herein by reference to disclose and describe the methods and/or materials in connection with which the publications are cited. The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided may be different from the actual publication dates which may need to be independently confirmed.

[0065] It is noted that, as used herein and in the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise. It is further noted that the claims may be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as “solely,” “only” and the like in connection with the recitation of claim elements, or use of a “negative” limitation.

[0066] As will be apparent to those of skill in the art upon reading this disclosure, each of the individual embodiments described and illustrated herein has discrete components and features which may be readily separated from or combined with the features of any of the other several embodiments without departing from the scope or spirit of the present invention.

DETAILED DESCRIPTION

Gamma Secretase Inhibitors

[0067] A variety of GSIs have been developed as potential clinical candidates for Alzheimer’s Disease and cancer indications. (See Kreft et al, at 6171). DAPT was one of the earliest GSIs identified. Modifications of DAPT led to clinical candidates.

[0068] Semagacestat is (2S)-2-hydroxy-3-methyl-N-[(1S)-1-methyl-2-oxo-2-[[(1S)-2,3,4,5-tetrahydro-3-methyl-2-oxo-1H-3-benzazepin-1-yl]amino]ethyl]-butamide, a small molecule gamma secretase inhibitor that was initially developed for the treatment of Alzheimer’s Disease. (See U.S. Pat. No. 7,468,365). Semagacestat is known to exist in a number of polymorphic forms, including a dihydrate and at least two anhydrate forms. (Id., See also U.S. Pat. No. 8,299,059). See also, Yi et al, DMD (2010) 38:554–565; http://doi:10.1124/dmd.109.030841.

[0069] Nirogacestat (aka PF-03084014) is ((S)-2-((S)-5,7-difluoro-1,2,3,4-tetrahydronaphthalen-3-ylamino)-N-(1-(2-m- ethyl-1-(neopentylamino)propan-2-yl)-1H-imidazol-4-yl)pentanamide, a small molecule gamma secretase inhibitor that was developed for cancer indications. It is available as a hydrobromide salt (www.medchemexpress.com), and exists is solid state forms. (See U.S. Pat. No. 10,590,087). See Wei P, et al. Evaluation of selective gamma-secretase inhibitor PF-03084014 for its antitumor efficacy and gastrointestinal safety to guide optimal clinical trial design. Mol Cancer Ther. 2010 Jun;9(6):1618-28; and Kumar, S., et al., Clinical Activity of the gamma-secretase inhibitor PF-03084014 in adults with desmoid tumors (aggressive fibromatosis). J. Clin Oncol. (2017) May 10;35(14):1561-1569. Seventeen patients were dosed at 50 mg orally twice a day in 3-week cycles for six cycles (18 weeks).

[0070] MK-0752 is a small molecule gamma-secretase inhibitor being studied for cancer indications. Phase 1 clinical data is described in Krop I, et al. Phase I pharmacologic and pharmacodynamic study of the gamma secretase (Notch) inhibitor MK-0752 in adult patients with advanced solid tumors. J Clin Oncol. 2012;30(19):2307-2313. In this study, of 103 patients who received MK-0752, 21 patients received a continuous once-daily dosing at 450 and 600 mg; 17 were dosed on an intermittent schedule of 3 of 7 days at 450 and 600 mg; and 65 were dosed once per week at 600, 900, 1,200, 1,500, 1,800, 2,400, 3,200, and 4,200 mg. The most common drug-related toxicities were diarrhea, nausea, vomiting, and fatigue. Toxicity was found to be schedule dependent, with weekly dosing deemed generally well-tolerated. See also, Matthews et al, Journal of Chromatography B, 863 (2008) 36-45; https://doi:10.1016/j.jchtomb.2007.12.025.

[0071] RO-4929097 is a small molecule gamma secretase inhibitor being studied for cancer indications. See, e.g., Tolcher AW, Messersmith WA, Mikulski SM et al. Phase I study of RO4929097, a gamma secretase inhibitor of Notch signaling, in patients with refractory metastatic or locally advanced solid tumors. J Clin Oncol 2012; 30: 2348-2353; Wu et al, Journal of Chromatography B, 879 (2011) 1537-1543. In this study, patients received escalating doses of RO4929097 orally on two schedules: (A) 3 consecutive days per week for 2 weeks every 3 weeks; (B) 7 consecutive days every 3 weeks; and (C) continuous daily dosing. Toxicities included fatigue, thrombocytopenia, fever, rash, chills, and anorexia. The study concluded that RO4929097 was well tolerated at 270 mg on schedule A and at 135 mg on schedule B; and the safety of schedule C was not fully evaluated.

[0072] Crenigascestat (aka LY3039478) is a small molecule gamma secretase inhibitor being studied for cancer indications. See Yuen E., et al., Evaluation of the effects of an oral notch inhibitor, crenigacestat (Ly3039478), on QT interval, and bioavailability studies conducted in healthy subjects. Cancer Chemother PHarmacol. 2019 Mar;83(3):483-492.In this study_crenigacestat was administered to healthy subjects as single 25, 50, or 75 mg oral doses or as an intravenous dose of 350 .Math.g .sup.13C.sup.15N.sup.2H.Math.crenigacestat.

[0073] In a Phase III trial in Alzheimer’s Disease, semagacestat, dosed at 100 mg and 140 mg daily, did not improve cognitive status and patients on the highest dose showed a significant worsening of cognitive ability. Semagacestat was also associated with more adverse events, including skin cancers and infections. Doody, R.S., et al., N Engl J Med 369;4: 341-350 (Jul. 25, 2013). An earlier Phase I study reported subjects dosed with 5 mg, 20 mg or 40 mg daily for 14 days showed adverse events similar to placebo, while 2 of 7 subjects receiving a 50 mg daily dose reported adverse events that may have been drug related. Siemers E. Skinner M, Dean RA, et al. Safety, tolerability, and changes in amyloid beta concentrations after administration of a gamma-secretase inhibitor in volunteers. Clin Neuropharmacol. 2005;28(3):126-132.

[0074] Because of evidence that Notch-signaling is dysregulated in numerous malignancies, GSIs have been developed as potential cancer therapeutics, as monotherapies or in combination with other agents. See, e.g., Takebe N, Nguyen D, Yang SX. Targeting notch signaling pathway in cancer: clinical development advances and challenges. Pharmacol Ther. 2014 Feb;141(2):140-9. doi: 10.1016/j.pharmthera.2013.09.005. Epub 2013 Sep 27; Shao H, Huang Q, Liu ZJ. Targeting Notch signaling for cancer therapeutic intervention. Adv Pharmacol. 2012;65:191-234. doi: 10.1016/B978-0-12-397927-8.00007-5.

[0075] It has now been found that low dose GSIs are effective in the treatment of respiratory diseases characterized by mucus hypersecretion, and are effective at doses allowing therapeutic activity while avoiding or minimizing the adverse effects previously associated with this class of molecules. As used herein, “low dose” of a GSI refers to a dose that is an effective amount to treat the respiratory disease characterized by mucus hypersecretion and is a lower dose as compared to a dose of the GSI suitable for administering to a patient suffering from a neurodegenerative disorder, an oncology disorder, or a respiratory disease not characterized by mucus hyper-secretion. For example, a low dose would be a dose yielding a peak plasma level in the submicromolar range. It will be appreciated that a low dose of a GSI may be administered as a single daily dose, multiple doses per day (e.g.. 2 or 3 doses per day), intermittent, or weekly, with the dosing regimen dependent on the dosage form (e.g., immediate release or controlled release), and the needs of the patient. Administration may be for an extended period of time, intermittent, or may be for a limited amount of time, with administration repeated if and to the extent determined by a patient’s medical provider. For example, a GSI may be provided daily for 1, 3, 5, 7, 10, 14, 18, 21, 24, 28 or 30 days, and then stopped. In some embodiments, the GSI is administered intermittently, such as every 3 days, or weekly. It will be appreciated that references to daily dosing amounts herein can be accomplished by dosing regimens other than daily, e.g., a weekly dose of 35 mg would correspond to a daily dose of 5 mg/day. Likewise, slow-release formulations, such as depots or patch formulations are known in the art and can be utilized to provide doses equivalent to the daily doses described herein. The GSI dosing regimen may be repeated if necessary.

[0076] For example, each of the GSIs semagacestat, nirogacestat (PF-03084014), RO-4929097 and MK-0752. when administered in the nanomolar range to human nasal epithelial cells is effective in blocking Notch signaling, driving differentiation towards MCCs, and rescuing conditions associated with excessive goblet cell mucus secretion. Hence, relatively low systemic levels of GSIs may provide effective treatment for respiratory conditions associated with mucus hypersecretion, while avoiding or minimizing adverse events observed with higher doses.

[0077] It has further been found that GSIs administered in combination with a CFTR modulator is effective in correcting epithelial cell dysfunction in cystic fibrosis cell-based model systems (primary cells from patients), in contrast to certain prevailing concepts, and indeed the combination may be synergistic in improving CFTR ion channel function and epithelial cell correction. For example, various GSIs have now been shown to not interfere with CFTR ion channels and not inhibit effects of CFTR modulators on CFTR ion channels in cystic fibrosis airway epithelial cells. It has been found that GSI treatment, surprisingly, improves airway surface liquid (ASL) reabsorption of CF cells to the same degree as CFTR modulator drugs. Further evidence suggests that GSI treatment may be synergistic with CFTR modulator treatment, thereby allowing the potential to decrease doses of either or both drugs, and further reducing potential toxicities of each.

[0078] GSIs may be administered by pharmaceutical dosage forms known in the art, including but not limited to oral solid dosages, oral liquids, injection, transdermal patch, and inhalation. Dosage forms may be formulated with excipients and other compounds to facilitate administration to a subject and to maintain shelf stability. See “Remington’s Pharmaceutical Sciences” (Mack Publishing Co., Easton, PA). Oral pharmaceutical formulations include tablets, minitablets, pellets, granules, capsules, gels, liquids, syrups and suspensions. Preferably, a GSI is administered orally, typically via oral solid dosage, although oral liquids may be desirable for certain populations that have difficulty with tablets and capsules, such as pediatric and elderly patients. Oral dosage forms may be immediate release or controlled release.

[0079] Tablet forms of semagacestat are known in the art. (See U.S. Pat. No. 8,299,059). Upon oral administration, semagacestat is reported to have a half-life of approximately 2.5 hours. Hence, in one embodiment of the invention, semagacestat may be provided as an immediate release formulation. Immediate release semagacestat may be provided as a single daily dose, or divided into multiple daily dosages which may be administered 2, 3, 4 or more times per day. In another embodiment of the invention, semagacestat is provided as an extended release formulation. An extended release formulation may provide patient convenience by reducing daily administrations, and may improve patient compliance. Further, controlled release formulations of the present invention may be useful in reducing serum peaks and troughs, thereby potentially reducing adverse events.

[0080] Oral controlled release formulations are known in the art and include sustained release, extended release, delayed release and pulsatile release formulations. See “Remington’s Pharmaceutical Sciences” (Mack Publishing Co.. Easton, PA). The active agent may be formulated in a matrix formulation with one or more polymers that slow release of the drug from the dosage form, including hydrophilic or gelling agents, hydrophobic matrices, lipid or wax matrices and biodegradable matrices. The active agent may be formulated in the form of a bead, for example with an inert sugar core, and coated with known excipients to delay or slow release of the active agent by diffusion. Enteric coatings are known in the art for use in delaying release of an active agent until the dosage form passes from the low pH environment of the stomach to the higher pH environment of the small intestine, and my include methyl acrylate-methacrylic acid copolymenrs, cellulose acetate phthalate (CAP), cellulose acetate succinate, hydroxypropyl methylcellulose phthalate, hydroxypropyl methylcellulose acetatate succinate, polyvinyl acetate phthatlate (PVAP), shellac, sodium alginate, and cellulose acetate trimellitate.

[0081] In some embodiments, the GSI is selected from the group consisting of semagacestat, avagacestat, GS-1, DBZ, L-685,458, BMS-906024, crenigascestat, MRK 560, nirogacestat, RO-4929097, MK-0752, itanapraced, LY-3056480, fosciclopirox, tarenflurbil, and begacestat.

[0082] In some embodiments, the GSI is selected from semagacestat., nirogacestat, MK-0752, RO-492907, or crenigacestat. In one embodiment, the GSI is semagacestat.

[0083] In some embodiments, a method is provided for treating a respiratory disease characterized by mucus hyper-secretion comprising systemically administering to a patient in need thereof about 0.1 mg to about 50 mg semagacestat daily wherein the oral administration of semagacestat is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. Preferably, semagacestat is systemically administered at dosages of from about 0.5 mg to about 40 mg daily, or from about 0.5 mg to about 30 mg daily, and most preferably of from about 0.5 mg to about 20 mg daily, or from 0.5 mg to about 10 mg daily. For example, semagacestat may be administered in about 0.1 mg, 0.25 mg, 0.5 mg, 1 mg, 2.5 mg, 5 mg, 10 mg, 15 mg, 20 mg, 25 mg, 30 mg, 35 mg, 40 mg, 45 mg or 50 mg daily.

[0084] In another embodiment, methods are provided for treating a respiratory disease characterized by mucus hypersecretion comprising systemically administering to a patient in need thereof about 5 .Math.g to about 1 mg/kg daily, preferably from about 50 to about 100 .Math.g/kg semagacestat daily.

[0085] In an embodiment of the invention, a method is provided for treating a respiratory disease characterized by mucus hyper-secretion comprising systemically administering to a human patient in need of such treatment a therapeutically effective amount of semagacestat, wherein said patient’s semagacestat plasma concentration at steady state following multiple dose administration comprises an AUC (area under the curve) less than 1220 ng•hr/mLwherein the systemic administration of semagacestat is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. In some embodiments, upon multiple dose administration, said patient’s steady state semagacestat plasma concentration comprises an AUC less than 1220 ng•hr/mL, less than 600 ng•hr/mL, or less than 250 ng•hr/mL.

[0086] In some embodiments, a method is provided for treating a respiratory disease characterized by mucus hyper-secretion comprising systemically administering to a patient in need thereof about 0.1 mg to about 50 mg nirogacestat daily wherein the oral administration of semagacestat is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. In some embodiments the nirogacestat is systemically administered at dosages of from about 0.5 mg to about 40 mg daily, or from about 0.5 to about 30 mg, or of from about 0.5 mg to about 20 mg daily.

[0087] In another embodiment, methods are provided for treating a respiratory disease characterized by mucus hypersecretion comprising systemically administering to a patient in need thereof about 8 .Math.g to about 0.9 mg/kg daily, preferably from about 10 to about 300 .Math.g/kg nirogacestat daily.

[0088] In some embodiments, a method is provided for treating a respiratory disease characterized by mucus hyper-secretion comprising systemically administering to a patient in need thereof about 0.1 mg to about 20 mg RO-4929097 daily wherein the oral administration of RO-4929097 is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. In some embodiments the RO-4929097 is systemically administered at dosages of from about 0.1 to about 10 mg daily, or from about 0.5 mg to about 10 mg daily, or of from about 0.1 mg to about 5 mg daily.

[0089] In another embodiment, methods are provided for treating a respiratory disease characterized by mucus hypersecretion comprising systemically administering to a patient in need thereof about 5 .Math.g to about 0.4 mg/kg daily, preferably from about 50 to about 100 .Math.g/kg RO-4929097 daily.

[0090] In some embodiments, a method is provided for treating a respiratory disease characterized by mucus hyper-secretion comprising systemically administering to a patient in need thereof about 0.1 mg to about 40 mg MK-0752 daily wherein the oral administration of MK-0752 is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. In some embodiments, the MK-0752 is administered at a dose in the range of from about 0.1 to about 30 mg daily, or from about 0.1 mg to about 20 mg daily, and most preferably of from about 0.1 mg to about 10 mg daily.

[0091] In another embodiment, methods are provided for treating a respiratory disease characterized by mucus hypersecretion comprising systemically administering to a patient in need thereof about 2.5 .Math.g to about 0.6 mg/kg daily, preferably from about 2.5 to about 500 .Math.g/kg MK-0752 daily.

[0092] In some embodiments, the respiratory disease characterized by mucus hypersecretion is selected from the group consisting of cystic fibrosis, chronic obstructive pulmonary disease, primary ciliary dyskinesia, chronic bronchitis, asthma, idiopathic and secondary bronchiectasis, bronchiolitis obliterans, Idiopathic pulmonary fibrosis and other fibrotic lung disorders and respiratory infection, including exacerbations in chronic respiratory disorders and mucus accumulation in response to acute infection. In some embodiments, the respiratory disease is cystic fibrosis. In other embodiments, the respiratory disease is chronic obstructive pulmonary disease.

[0093] In some embodiments, the GSI is administered by inhalation. In preferred embodiments, the GSI is administered by oral administration. In one embodiment, the GSI is provided in an immediate release solid oral dosage form. In another embodiment, the GSI is provided in a controlled release solid oral dosage from. In a further embodiment, the GSI is provided in a liquid dosage form. In a further embodiment, the GSI is provided in an inhalation dosage form.

Cystic Fibrosis Combination Therapy

[0094] CFTR modulator drugs have provided a significant advance in the treatment of cystic fibrosis. They do not, however, address damage that occurs to the lung epithelium due to cystic fibrosis. Further, CFTR modulators are limited to use in patients that have the specific CFTR mutations addressed by the particular CFTR modulator drug.

[0095] The cell type or types that most express functional CFTR is not defined. It has been suggested that a rare cell type named ionocyte might be the major source of CFTR expression and therefore activity. Plasschaert, L.W., et. al., A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte. Nature (2018) 560: 377-381; https://doi.org/10.1038/s41586-018-0394-6. Furthermore, it was suggested that the ionocyte population is expected to be diminished upon Notch inhibition and CFTR activity likewise decrease. (Id. at 380). Other data published more recently suggests that a diversity of cell types express varying levels of CFTR Carraro, G., et al., Nat. Med. (2021) May;27(5):806-814. Doi: 10.1038/s41591-021-01332-7.Epub 2021 May 5. In contrast to published assertions of GSI interference with CFTR activity, it has now been found that GSI treatment does not diminish CFTR activity. Rather, it has been found that GSI treatment, surprisingly, improves ciliary beat frequency (CBF) and mucus transport of CF cells to the same degree as CFTR modulator drugs. Further evidence suggests that GSI treatment may be synergistic with CFTR modulator treatment, thereby allowing the potential to decrease doses of either or both drugs, and further reducing potential toxicities of each.

[0096] Accordingly, methods of the invention address the dysfunction present in cystic fibrosis airway and other epithelial cells that lead to mucus hypersecretion (and often infection), by promoting differentiation of MCCs and reduction of mucus secreting cells, and enabling improved mucociliary clearance. Administration of a GSI may improve epithelial function in cystic fibrosis without regard to the CFTR mutations causing the underlying disease. Hence, in the treatment of cystic fibrosis, a GSI may be administered alone or in combination with any CFTR modulator or combination of CFTR modulators.

[0097] In some embodiments of the invention, methods are provided for treating cystic fibrosis comprising administering to a patient in need thereof a therapeutically effective amount of a GSI and a CFTR modulator. The GSI may be administered prior to, after or concurrently with the CFTR modulator. In some embodiments, the GSI is administered orally to a patient taking a CFTR modulator. The GSI may be provided in a single course of treatment or may be provided intermittently in combination with a CFTR modulator dosing regimen.

[0098] For example, a CFTR modulator may be administered daily and a GSI may be administered daily for 1, 3, 5, 7, 10, 14, 18, 21, 24, 28 or 30 days, and then stopped. In some embodiments, the GSI is administered intermittently, such as every 3 days, or weekly. It will be appreciated that references to daily dosing amounts herein can be accomplished by dosing regimens other than daily, e.g., a weekly dose of 35 mg would correspond to a daily dose of 5 mg/day. Likewise, slow-release formulations, such as depots or patch formulations are known in the art and can be utilized to provide doses equivalent to the daily doses described herein. The GSI dosing regimen may be repeated if necessary. In some embodiments, the GSI is selected from semagacestat, nirogacestat, MK-0752, RO-492907, or crenigacestat. In one embodiment, the GSI is semagacestat.

[0099] CFTR modulators useful in the present invention include CFTR potentiators, correctors, premature stop codon suppressors, amplifiers and combinations thereof. Currently marketed CFTR modulators include ivacaftor, lumacaftor, tezacaftor and elexacaftor and combinations. Ivacaftor is marketed in tablet and granule form as KALYDECO. (See U.S. Pat. Nos. 7,495,103 and 8,754,224). Ivacaftor and tezacaftor are marketed as SYMDEKO. (See U.S. Pat. Nos. 7,745,789; 7,776,905; 8,623,905 and 10,239,867). A combination of lumacaftor and ivacaftor is marketed as ORKAMBI. (See U.S. Pat. Nos. 8,507,534 and 10,597,384). A combination of elexacaftor, ivacaftor and tezacaftor (“ETI”) is marketed as TRIKAFTA. Additional CFTR modulators that may be used in the present invention are in development. (See, e.g., U.S. Pat. Nos. 10,647,717; 10,604,515;10,568,867; 10,428,017; 10,399,940; 10,259,810; 10,118,916; 9,895,347; 10,550,106; 10,548,878; 10,392,378; 10,494,374; 10,377,762; 10,450,273; 9,890,149 and 10,258,624).

[0100] In an embodiment of the invention, methods are provided for treating cystic fibrosis by administration of a therapeutically effective amount of a GSI and a CFTR modulator. In another embodiment, a method of treating cystic fibrosis is provided in which a GSI is systemically administered to a patient being administered or in need of administration of a CFTR modulator. The GSI may be provided concurrently, prior to or after administration of the CFTR modulator. In some embodiments, the GSI is provided intermittently in combination with a CFTR modulator dosing regimen.

[0101] In one embodiment, a method of treating cystic fibrosis in a patient being administered or in need administration of a CFTR modulator is provided, comprising systemically administering to such patient about 0.1 mg to about 50 mg semagacestat daily wherein the oral administration of semagacestat is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. In some embodiments, semagacestat is systemically administered at dosages of from about 0.5 mg to about 40 mg daily. In some embodiments, semagacestat is administered at a dosage of from about 0.5 mg to about 20 mg daily.

[0102] In one embodiment, a method of treating cystic fibrosis in a patient taking a CFTR modulator is provided comprising systemically administering semagacestat to a patient in need thereof about 5 .Math.g to 1 mg/kg daily, preferably from about 50 to 100 .Math.g/kg daily.

[0103] In some embodiments, semagacestat is provided orally to a patient taking a CFTR modulator wherein said patient’s semagacestat plasma concentration at steady state following multiple dose administration comprises an AUC (area under the curve) less than 1220 ng•hr/mL, wherein the systemic administration of semagacestat is effective in reducing mucus in such patient’s lungs or preventing mucus accumulation in such patient’s lungs. In some embodiments, upon multiple dose administration, said patient’s steady state semagacestat plasma concentration comprises an AUC less than 1220 ng•hr/mL, less than 600 ng•hr/mL, or less than 250 ng•hr/mL. Steady state semagacestat levels may be determined following about 1 week or about 2 weeks or more of administering the therapeutically effective amount of semagacestat.

[0104] In some embodiments, a method of treating cystic fibrosis in a patient being administered or in need administration of a CFTR modulator is provided comprising systemically administering to a patient in need thereof about 0.1 mg to about 50 mg nirogacestat daily wherein the oral administration of nirogacestat is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. In some embodiments the nirogacestat is systemically administered at dosages of from about 0.5 mg to about 40 mg daily, or from about 0.5 to about 30 mg, or of from about 0.5 mg to about 20 mg daily.

[0105] In another embodiment, a method of treating cystic fibrosis in a patient taking a CFTR modulator is provided comprising systemically administering to a patient in need thereof about 8 .Math.g to about 0.9 mg/kg daily, preferably from about 10 to about 300 .Math.g/kg nirogacestat daily.

[0106] In some embodiments, a method of treating cystic fibrosis in a patient taking a CFTR modulator is provided comprising systemically administering to a patient in need thereof about 0.1 mg to about 20 mg RO-4929097 daily wherein the oral administration of RO-4929097 is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. In some embodiments the RO-4929097 is systemically administered at dosages of from about 0.1 to about 10 mg daily, or from about 0.5 mg to about 10 mg daily, or of from about 0.1 mg to about 5 mg daily.

[0107] In another embodiment, a method of treating cystic fibrosis in a patient taking a CFTR modulator is provided comprising systemically administering to a patient in need thereof about 5 .Math.g to about 0.4 mg/kg daily, preferably from about 50 to about 100 .Math.g/kg RO-4929097 daily.

[0108] In some embodiments, a method of treating cystic fibrosis in a patient taking a CFTR modulator is provided comprising systemically administering to a patient in need thereof about 0.1 mg to about 40 mg MK-0752 daily wherein the oral administration of MK-0752 is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs. In some embodiments, the MK-0752 is administered at a dose in the range of from about 0.1 to about 30 mg daily, or from about 0.1 mg to about 20 mg daily, and most preferably of from about 0.1 mg to about 10 mg daily.

[0109] In another embodiment, a method of treating cystic fibrosis in a patient taking a CFTR modulator is provided comprising systemically administering to a patient in need thereof about 2.5 .Math.g to about 0.6 mg/kg daily, preferably from about 2.5 to about 500 .Math.g/kg MK-0752 daily.

[0110] Preferably, the GSI is provided by oral administration. The GSI may be provided as an immediate release oral dosage form, or as a controlled release oral dosage form.

[0111] Generally, the human subject that is treated by methods of the invention, e.g., as described above, is one that has been diagnosed as having a respiratory disease characterized by mucus hypersecretion. In some instances, the respiratory disease characterized by mucus hypersecretion for which the subject is diagnosed as having is selected from the group consisting of cystic fibrosis, chronic obstructive pulmonary disease, primary ciliary dyskinesia, chronic bronchitis, asthma, idiopathic and secondary bronchiectasis, bronchiolitis obliterans. Idiopathic pulmonary fibrosis and other fibrotic lung disorders and respiratory infection, including exacerbations in chronic respiratory disorders, and mucus accumulation in response to acute infection. In some embodiments, the subject is a subject diagnosed as having cystic fibrosis. In other embodiments, the subject is a subject diagnosed as having chronic obstructive pulmonary disease.

[0112] The following examples are offered by way of illustration and not by way of limitation.

EXAMPLES

Example 1: Treatment of HNECs With GSIs

[0113] Air-Liquid Interface (ALI) cultures were prepared as described in Vladar EK, Nayak JV, Milla CE, Axelrod JD. Airway epithelial homeostasis and planar cell polarity signaling depend on multiciliated cell differentiation. JCI Insight. 2016;1(13);e88027. Human nasal epithelial cells (HNECs) were generated from human sinonasal epithelial brushings or from tissue obtained from patients undergoing endoscopic sinus surgery at Stanford Hospital and cultured as described in Vladar et al.

[0114] Cultures were treated with varying doses of semagacestat. DAPT (Abcam) was used (0.5 .Math.g) as a positive control for GSI activity. Cultures were labeled at ALI+21d with anti-acetylated α-tubulin (green) and ECAD (red) antibodies to mark cilia and epithelial junctions. Results shown in FIG. 1 demonstrate dose response of HNECs treated with semagacestat, and show effective conversion to MCCs with nanomolar concentrations of semagacestat.

[0115] Using ECAD, we counted the total number of cells, and anti-acetylated α-tubulin was used to count the number of MCCs (does not include immature MCCs that have been fated but have not made cilia yet, as our focus was on “functional” MCCs). The ratio was defined as MCC number/total luminal cells. Counting was done on one representative image from 5 culture replicates from a single donor. Results shown in FIG. 2 show that both 1 .Math.M DAPT and semagacestat in doses ranging from 500 nM to 31.25 nM produced significant increases in this ratio (all p <0.01 by ANOVA with post hoc Dunnett’s multiple comparisons test).

[0116] The effective semagacestat doses correspond to more than two orders of magnitude lower than those used in human Alzheimer’s Disease trials.

Example 2: In Vivo Mouse Models

[0117] 10-40 week age-matched male and female Foxj1-GFP mice were given semagacestat or vehicle by intraperitoneal (IP) administration. In the first experiment, semagacestat was administered at 0.1 mg/kg and 1 mg/kg twice daily for three days and compared to vehicle alone. Mice were sacrificed on day 7. FIG. 3 demonstrates the method of evaluating airway cellular composition. In approximately similar sized, PFA-fixed airways, nuclei (red; DAPI) were scored either as MCCs (green; GFP) or non-ciliated cells (absence of GFP). Acetylated tubulin (blue) marks cilia. Samples were blinded for treatment group prior to scoring. FIG. 5 shows an increase in the ratio of ciliated to non-ciliated cells following 3 days of systemic (IP) semagacestat treatment at both the low and high doses.

[0118] In the second experiment, vehicle and semagacestat were administered once daily for 5 consecutive days per week for three weeks, with a semagacestat dose of 1 mg/kg. An important observed toxicity in multiple GSI clinical trials, including large Alzheimer’s Disease trials, was gastrointestinal toxicity. As a surrogate measure of GI toxicity, we monitored body weight throughout the experiment.

[0119] Body weight was measured on Days 1, 9, 24 and 30, and mice were sacrificed on day 31. No mortality or ill effects were noted in any group. FIG. 4 shows body weight at days 9, 24 and 30 and demonstrates no significant difference in treatment groups as compared to vehicle controls. FIG. 6 shows the significant increase in the ratio of ciliated to non-ciliated cells after 3 weeks of systemic (IP) semagacestat.

[0120] In the three-day treatment, a dose response trend was observed, with the high dose reaching statistical significance (FIG. 5). The three-week treatment response at higher dose once a day was highly significant (FIG. 6).

Example 3: Dependence of Multiciliated Cell Formation on Timing of Treatment in Differentiating and Mature Airway Epithelia

[0121] Primary human airway epithelial cells were treated with DAPT and LY45139: i) during proliferation only (ALI-5 to -1d), ii) during differentiation only (ALI+0 to +21d) or iii) continuously during the entire culture duration, followed by labeling at ALI+21d with anti-acetylated α-Tubulin (green) and ECAD (red) antibodies. GSI treatment during multiciliated cell differentiation (differentiation only and continuous treatments) increased MCC cell numbers. Results shown in FIG. 7 show that GSI treatment during proliferation only had no effect on differentiation, nor a detrimental effect on subsequent differentiation or overall epithelial structure. FIG. 8 shows quantitation (MCCs per total luminal cells) of data shown in FIG. 7.

[0122] Mature (ALI+30d) primary human airway epithelial cells were treated with DAPT and semagacestat for one (ALI+30 to +37d) or two weeks (ALI+30 to +44d), then labeled with anti-acetylated α-Tubulin (green) and ECAD (red) antibodies. Results shown in FIG. 9 show that GSI treatment induces the formation of additional multiciliated cells in mature cultures, while untreated cultures do not differentiate any more multiciliated cells. FIG. 10 shows the quantitation of data shown in FIG. 9.

[0123] Primary human airway epithelial cells were treated with DAPT and LY45139 during differentiation only (ALI+0 to +21d) from either the apical or basal surface, then labeled at ALI+21d with anti-acetylated α-Tubulin (green) and ECAD (red) antibodies. Results shown in FIG. 11 show that GSI treatment induces ciliated cell formation via both apical and basal application. Apical treatment eliminates the air-liquid interface, which results in the poor epithelial structure and multiciliated cell differentiation in the untreated cultures, which is partially rescued by GSI treatment. FIG. 12 shows quantitation of data shown in FIG. 11. This result suggests that both systemic exposure and inhalation exposure are likely to be effective in vivo.

[0124] The results show that treatment during or after differentiation increases the ratio of MCCs to total cells. Treatment during proliferation (prior to differentiation) has no apparent effect, either beneficial or adverse.

Example 4: IL-13 Induced Chronic Inflammation Model

[0125] Administration of IL-13 to ALI cultures induces goblet cell hyperplasia and is a useful model of chronic inflammation. ALI HNEC cultures were prepared as described in Vladar et al. ALI cultures were treated with and without administration of Il-13 on days 7-14. DAPT (1 um), semagacestat (125 nm) or vehicle control were administered on days 14-21. PFA-fixed cultures were stained for Muc5AC (red; mucin producing secretory cells), Acetylated tubulin (green; MCCs) and E-Cadherin (blue to reveal cell boundaries).

[0126] FIG. 13 shows the effect of semagacestat and DAPT treatment in an ALI model of chronic inflammation. HNEC cultures from a CF patient were treated with IL-13 from ALI+7 to 14 to induce inflammation. DAPT and semagacestat increase the percentage off MCCs in controls (left). IL-13 treatment increases the percentage of mucin positive secretory cells and decreases the percentage of MCCs. Subsequent DAPT or semagacestat treatment rescues cell composition, increasing the percentage of MCCs and decreasing the percentage of mucin positive secretory cells.

[0127] FIG. 14 shows the quantitation of MCCs per total luminal cells of the data from FIG. 13.

Example 5: Representative Ussing Chamber Tracings

[0128] Cells grown at an air liquid interface were mounted on a holding slider and inserted on an Ussing chamber for electrophysiological short circuit current (I.sub.sc) measurements. Solutions in the serosal and mucosal bath were prepared so that a chloride gradient was established between both sides. After stable baseline current recordings were obtained, agonists were added in the following order: amiloride (10 .Math.M) to block sodium channel activity, forskolin (10 .Math.M) to stimulate CFTR, Ivacaftor (10 .Math.M) to potentiate CFTR activity, and CFTRinh-172 (20 .Math.M) to block CFTR current. For each agonist signals were monitored until a plateau in current was noted before adding the next agonist. The delta-Isc in response to CFTR.sub.inh-172 was used as our main read out for CFTR-mediated chloride transport. Results are shown in FIG. 15.

Example 6: Electrophysiological Assay for CFTR Activity

[0129] To assess the effect of GSIs (DAPT, Semagacestat) on CFTR function in cultured epithelia, HNECs from CF patients and non-CF controls were collected and grown at air-liquid interface to maturity (+21d) according to Vladar et al. Cultures were then treated with DAPT, Semagacestat, the CFTR modulator Lumacaftor or vehicle control added to basal media 3x per week for 2 weeks. Filter inserts were then assessed for short circuit current (Isc) against a chloride gradient in Ussing chambers to assess CFTR activity. FIG. 16 shows representative tracings. FIG. 17 quantifies CFTR channel activity as assessed by inhibition of current after addition of CFTR.sub.inh172 for two wild-type control cultures and two CF patient-derived cultures (genotypes: rare/rare = W1282X [class I]/I1234V [class II] and F508Δ/F508Δ). Note that in all cases, CFTR currents in semagacestat treated cultures are equal to or greater than in control conditions.

Example 7: Mucus Production in Human CF Cells

[0130] In FIG. 18, duplicate cultures from the experiment in Example 6 were PFA fixed and stained for Muc5AC (red; mucus) and Acetylated tubulin (green; MCCs). Note the thick ropes of mucus (red) in vehicle control treated CF cultures that were resistant to washing. Semagacestat treated cultures revealed much less mucus without ropes. The effect was observed in homozygous F508Δ and W1282X/I1234V (a rare modulator-responsive genotype) cells under treatment with the CFTR modulator combination of lumacaftor and ivacaftor.

Example 8: Combination of Semagacestat and CFTR Modulator

[0131] To assess the effects of combining semagacestat and CFTR modulator treatment, wild-type control and CF (F508Δ/F508Δ) HNEC cultures were grown to maturity with or without semagacestat from ALI +0-21d and cultures were treated with or without Lumacaftor (VX-809) from ALI +19-21d and Ivacaftor (VX-770) for ten minutes prior to fixation. PFA fixed membranes were then stained for Acetylated tubulin (green; MCCs) and E-Cadherin (red). Results are shown in FIG. 19. Note that combination treated cultures differentiated MCCs as well as or better than cultures treated with semagacestat alone.

[0132] FIG. 20 shows the quantitation of data for healthy patient and CF donor 1 in FIG. 19. In some cases, such as this one, the semagacestat-induced increase in MCC/total cell ratio is further increased by Lumacaftor. Although this was not consistently seen and is not statistically significant among all cultures we have quantified, we can conclude that no significant decrease in semagacestat-induced MCC response was seen. CF patients not eligible for Trikafta respond to semagacestat alone similarly to those on corrector therapy (not shown).

Example 9: GSI Treatment Induces Multiciliated Cell Formation in Cystic Fibrosis Epithelia

[0133] Primary healthy and cystic fibrosis airway epithelial cells were treated with semagacestat (LY45139) during differentiation only (ALI+0 to +21d) and labeled at ALI+21d with anti-acetylated α-Tubulin (green) and ECAD (red) antibodies. FIG. 21 shows that LY45139 was effective in increasing MCC/total cell ratio in CF patient-derived cultures. Restoration of a healthy MCC/total cell ratio is expected to improve mucociliary clearance.

Example 10: GSI Treatment Induces Structurally Normal Cilia in Healthy and CF Epithelia

[0134] FIG. 22 shows SEM of healthy and CF primary human airway epithelial cultures, showing that multiciliated cells formed under DAPT treatment are indistinguishable from those in untreated healthy cultures.

[0135] Mature (ALI+30d) primary cystic fibrosis human airway epithelial cells were treated with DAPT for one week (ALI+30 to +37d), then labeled with anti-acetylated α-Tubulin (green) and ECAD (red) antibodies. The results shown in FIG. 23 show that GSI treatment induces the formation of additional multiciliated cells in mature cystic fibrosis cultures, while untreated cultures do not differentiate any more multiciliated cells.

Example 11: Multiciliated Cell Formation is Induced by a Variety of GSIs

[0136] Primary human airway epithelial cells were treated during differentiation (ALI+0 to +21d) with DAPT and high and low concentrations of the GSIs LY45139, PF-03084014, RO-4929097 and MK-0752, and labeled at ALI+21d with anti-acetylated α-Tubulin (green) and ECAD (red) antibodies to mark cilia and epithelial junctions. Results are shown in FIG. 24. At high concentrations, GSIs disrupted epithelial structure, but at the low concentration all induced multiciliated cell formation, similar to DAPT. FIG. 25 shows the quantitation of MCCs per total luminal cells of the data shown in FIG. 24.

Example 12: CFTR Current is Not Diminished by GSI Treatment

[0137] We directly measured CFTR activity in cultures from CF patients treated with GSI with or without well-established effective in vitro doses of Trikafta (Elexcaftor/Tezacaftor/Ivacaftor or “ETI”). Veit, G., et al., JCI (2020) 10.1172/jci.insight.139983. Measurements were performed in Ussing chambers. Neither LY45139 (FIG. 26) nor MK04752 (FIG. 27) impaired the CFTR current induced by ETI. In some individual experiments, there appeared to be a synergistic effect in the combination treatment: GSI alone has no effect but the combination treatment produced a greater response than ETI alone. This effect was not consistently seen, but raised the possibility that synergy might be observed with lower ETI doses.

[0138] To examine this further, we tested MK04752 in combination with varying doses of ETI (FIG. 28). The results show that suboptimal doses of ETI appear to be potentiated by MK04752, a result that indicates synergy.

Example 13: GSI Treatment Has no Impact on Ionocyte Formation

[0139] Since ionocytes are of particular interest due to prior assertions about CFTR expression, in addition to measuring current, we assayed ionocyte prevalence with GSI treatment. Primary healthy airway epithelial cells were treated with DAPT during differentiation only (ALI+0 to +21d) and labeled at ALI+21d with anti-FOXI1 (green; an ionocyte specific marker), and acetylated α-Tubulin (red) antibodies and stained with DAPI (blue) to mark nuclei. FIG. 29 shows that both untreated and DAPT treated cultures contained a similar small number of FOXI1 positive nuclei, indicative of ionocytes. Therefore, the prior suggestion that ionocyte numbers would decrease with Notch inhibition appears not to be correct.

Example 14: Effect of Low Concentrations of the GSI MK-0752 and the CFTR Modulator Elexacaftor on Ciliary Beat Frequency (CBF)

[0140] Primary nasal epithelial cells from two CF donors (F508del homozygotes) were grown in filter inserts as in previous experiments to full differentiation. We evaluated whether lower doses of both MK-0752 and Elexacftor than doses used in the previous experiments could elicit a positive response in CBF as evidence for synergy between the two drugs. During differentiation, cultures received treatment with vehicle control or the GSI MK-0752 at 125 nM, or triple combination modulator combination with Elexacaftor at 100 nM, or both treatments combined. Once cells reached maturity, the apical surface was washed gently with PBS and then placed on an inverted microscope on a heated stage at 37° C. High speed video recording at 200X of the ciliated surface was performed to estimate the CBF in several regions. Average CBF in Hz for each condition are represented by the bars in FIG. 30. Both treatments demonstrated a significant effect in increasing the CBF (p < 0.001 for both vs control). Further, the increase in CBF elicited by the combined treatments was larger than with either drug alone (p < 0.005 for all comparisons) and demonstrating synergy as the increase in CBF was significantly above that expected by the simple addition of their independent effects (36% vs 28%, p =0.049).

[0141] FIG. 31 shows ciliary beat frequency (CBF) and cilium length of primary human epithelial cells treated with DAPT versus untreated controls. The results show that multiciliated cells differentiated in the presence of DAPT treatment had a modest, but significant increase in ciliary beat frequency, but showed no difference in cilium length.

Example 15: Airway Surface Liquid (ASL) Reabsorption Characteristic of CF is Attenuated by GSI Treatment to the Same Degree as CFTR Modulator Drugs

[0142] Primary nasal epithelial cells from two CF donors (F508del homozygote) were grown in filter inserts as in previous experiments to full differentiation. During differentiation they received treatment with vehicle control (blue), triple combination CFTR modulator (Elexcaftor/Tezacaftor/Ivacaftor; orange), the GSI semagacestat (LY-45139) (gray) or both treatments combined (yellow). Once they reached maturity, the apical surface was washed gently with PBS and then 30 .Math.l of PBS were added to the surface. The filter inserts were then weighed on a precision scale at times 0, 12 and 24 hours after fluid addition. The change in weight over time was taken as a surrogate for fluid reabsorption. Results are shown in FIG. 32. Control cells showed the typical pattern of ASL reabsorption over a 48-hour period, as opposed to treated cells that demonstrated significantly decreased reabsorption (p = <0.001 vs control). Notably, all drug treatments did not significantly differ in their effect on fluid reabsorption (p > 0.3 for all comparisons between drug treatments).

Example 16: Dramatic Improvement in Mucus Transport With Combined GSI and CFTR Modulator Treatment

[0143] Primary nasal epithelial cells from two CF donors (F508del homozygotes) were grown in duplicate as in previous experiments to full differentiation under treatment with vehicle control, triple combination CFTR modulator (Elexcaftor/Tezacaftor/Ivacaftor), the GSI semagacestat (LY-45139) or both treatments combined. Once mature, a 20 .Math.l suspension of 2 .Math.m latex beads was added to the apical surface and the insert cut for placement under a microscope fitted with a high-speed video recorder. Images were then acquired at 1000 fps to track bead movement as a reflection of mucus transport by the ciliated surface and distance travelled by individual beads estimated. FIG. 33 shows representative images from each treatment. Results shown in FIG. 34 show that control cells showed little movement of beads, reflective of poor mucus transport. Significant increases in movement were observed with either ETI or semagacestat treatment (p < 0.01 for either treatment vs control). Remarkable increases in transport were noted with the combination of ETI and semagacestat (p < 0.05 for comparisons against either treatment alone). This can be taken as demonstrating strong enhancement effects in mucociliary transport upon combination treatment, and predicts substantial benefit to patients on CFTR modulator therapy by adding GSI treatment.

[0144] Notwithstanding the appended claims, the disclosure is also defined by the following clauses:

[0145] 1. A method of treating a respiratory disease characterized by mucus hyper-secretion comprising: administering a low dose of a GSI to a human patient in need of such treatment; and

[0146] wherein the mucus in such patient’s lungs is reduced or mucus accumulation in such patient’s lungs is inhibited.

[0147] 2. The method of Clause 1 wherein the respiratory disease is selected from the group consisting of cystic fibrosis, chronic obstructive pulmonary disease, primary ciliary dyskinesis, chronic bronchitis, asthma, idiopathic and secondary bronchiectasis, bronchiolitis obliterans, idiopathic pulmonary fibrosis and other fibrotic lung disorders and respiratory infection, including exacerbations in chronic respiratory disorders and mucus accumulation in response to acute infection.

[0148] 3. The method of Clause 1 or 2 wherein the GSI is selected from the group consisting of semagacestat, avagacestat, GS-1, DBZ, L-685,458, BMS-906024, crenigascestat, MRK 560, nirogacestat, RO-4929097, MK-0752, itanapraced, LY-3056480, fosciclopirox, tarenflurbil, and begacestat.

[0149] 4. The method of Clause 3 wherein said GSI is selected from the group consisting of semagacestat., nirogacestat, MK-0752, RO-492907, or crenigacestat.

[0150] 5. The method of Clause 4 wherein the GSI is semagacestat.

[0151] 6. The method of Clause 4 wherein the GSI is MK-0752.

[0152] 7. The method of Clause 4 wherein the GSI is nirogacestat.

[0153] 8. The method of Clause 4 wherein the GSI is RO-492907.

[0154] 9. The method of Clause 4 wherein the GSI is crenigacestat.

[0155] 10. The method of Clause 2 wherein said administration of GSI is by oral administration.

[0156] 11. The method of Clause 2 wherein the respiratory disease is cystic fibrosis.

[0157] 12. The method of Clause 2 wherein the respiratory disease is chronic obstructive pulmonary disease.

[0158] 13. The method of Clause 5 wherein semagacestat is administered orally in an amount of from about 0.1 mg to about 50 mg daily.

[0159] 14. The method of Clause 13 wherein about 0.5 mg to about 40 mg of semagacestat is administered daily.

[0160] 15. The method of Clause 14 wherein about 0.5 mg to about 30 mg of semagacestat is administered daily.

[0161] 16. The method of Clause 15 wherein about 0.5 mg to about 20 mg of semagacestat is administered daily.

[0162] 17. The method of Clause 7 wherein the nirogacestat is administered orally in an amount of from about 8 ug to about 0.9 mg daily.

[0163] 18. The method of Clause 17 wherein about 10 ug to about 300 ug of nirogacestat is administered daily.

[0164] 19. The method of Clause 8 wherein the RO-492907 is administered orally in an amount of from about 0.1 mg to about 20 mg daily.

[0165] 20. The method of Clause 19 wherein about 0.1 mg to about 10 mg of RO-492907is administered daily.

[0166] 21. The method of Clause 20 wherein about 0.1 mg to about 5 mg of RO-492907 is administered daily.

[0167] 22. The method of Clause 6 wherein MK-0752 is administered orally in an amount of from about 0.1 mg to about 40 mg daily.

[0168] 23. The method of Clause 22 wherein about 0.1 mg to about 20 mg of MK-0752 is administered daily.

[0169] 24. The method of Clause 23 wherein about 0.1 mg to about 10 mg of MK-0752 is administered daily.

[0170] 25. A method of treating a respiratory disease characterized by mucus hyper-secretion comprising: [0171] systemically administering to a human patient in need of such treatment a therapeutically effective amount of semagacestat, wherein said patient’s semagacestat plasma concentration at steady state following about 1 week or after about 2 weeks or more of administering the therapeutically effective amount has an AUC less than 1220 ng•hr/mL, and [0172] wherein the administration of semagacestat is effective in reducing mucus in such patient’s lungs or inhibiting mucus accumulation in such patient’s lungs.

[0173] 26. The method of Clause 25 wherein the respiratory disease is selected from the group consisting of cystic fibrosis, chronic obstructive pulmonary disease, primary ciliary dyskinesis, chronic bronchitis, asthma, idiopathic and secondary bronchiectasis, bronchiolitis obliterans, idiopathic pulmonary fibrosis and other fibrotic lung disorders and respiratory infection, including exacerbations in chronic respiratory disorders and mucus accumulation in response to acute infection.

[0174] 27. The method of Clause 26 wherein said patient’s semagacestat plasma concentration at steady state following multiple dose administration comprises an AUC less than 600 ng•hr/mL

[0175] 28. The method of Clause 27 wherein said patient’s semagacestat plasma concentration at steady state following multiple dose administration comprises an AUC less than 250 ng•hr/mL

[0176] 29. The method of Clause 26 wherein the respiratory disease is cystic fibrosis.

[0177] 30. The method of Clause 26 wherein the respiratory disease is chronic obstructive pulmonary disease.

[0178] 31. A method of treating cystic fibrosis comprising: [0179] administering an effective amount of a GSI to a human patient being administered or need administration of one or more CFTR modulators, [0180] and wherein the mucus in such patient’s lungs is reduced or mucus accumulation in such patient’s lungs is inhibited upon administration of the GSI.

[0181] 32. The method of Clause 31 wherein the GSI is selected from the group consisting of semagacestat, avagacestat, GS-1, DBZ, L-685,458, BMS-906024, crenigascestat, MRK 560, nirogacestat, RO-4929097, MK-0752, itanapraced, LY-3056480, fosciclopirox, tarenflurbil, and begacestat.

[0182] 33. The method of Clause 32 wherein the GSI is selected from the group consisting of semagacestat., nirogacestat, MK-0752, RO-492907, or crenigacestat.

[0183] 34. The method of Clause 33 wherein the GSI is semagacestat.

[0184] 35. The method of Clause 33 wherein the GSI is MK-0752.

[0185] 36. The method of Clause 33 wherein the GSI is nirogacestat.

[0186] 37. The method of Clause 33 wherein the GSI is RO-492907.

[0187] 38. The method of Clause 33 wherein the GSI is crenigacestat.

[0188] 39. The method of Clause 31 wherein said administration of GSI is by oral administration.

[0189] 40. The method of Clause 31 wherein the CFTR modulator is a CFTR potentiator.

[0190] 41. The method of Clause 31 wherein the CFTR modulator is a CFTR corrector.

[0191] 42. The method of Clause 31 wherein the CFTR modulator is a CFTR amplifier.

[0192] 43. The method of Clause 31 wherein the CFTR modulator is selected from the group consisting of ivacaftor, lumacaftor, tezacaftor, elexacaftor and combinations thereof.

[0193] 44. The method of Clause 31 wherein the GSI is administered daily.

[0194] 45. The method of Clause 44 wherein the GSI is administered for up to thirty days and then stopped.

[0195] 46. The method of Clause 31 wherein the GSI is administered weekly.

[0196] 47. A method of treating cystic fibrosis comprising: [0197] administering an effective amount of a GSI to a human patient taking a CFTR modulator; [0198] wherein the GSI is selected from the group consisting of semagacestat, avagacestat, GS-1, DBZ. L-685,458, BMS-906024, crenigascestat, MRK 560, nirogacestat, RO-4929097, MK-0752, itanapraced, LY-3056480, fosciclopirox, tarenflurbil, and begacestat; [0199] wherein the CFTR modulator is selected from the group consisting of ivacaftor, lumacaftor, tezacaftor, elexacaftor and combinations thereof; and [0200] wherein the mucus in such patient’s lungs is reduced or mucus accumulation in such patient’s lungs is inhibited upon administration of the GSI.

[0201] 48. The method of Clause 47 wherein the GSI is selected from the group consisting of semagacestat., nirogacestat, MK-0752, RO-492907, or crenigacestat.

[0202] Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it is readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claims.