Fungicide composition and method for controlling disease of crop

Abstract

The present invention relates to a fungicide composition having excellent fungicidal activity, particularly a fungicide composition comprising isofetamid and mandestrobin as active ingredients, and a method for controlling a disease of a crop, which comprises applying isofetamid and mandestrobin to the crop.

Claims

1. A fungicide composition consisting of (a) isofetamid and mandestrobin as active ingredients and (b) a solid or liquid carrier, a surfactant, or other formulating adjuvant, wherein the weight ratio of isofetamid to mandestrobin is 1:1000 to 1000:1.

2. A method for controlling a disease of a crop, which consists of applying a fungicidal composition consisting of isofetamid and mandestrobin as active ingredients to the crop, wherein the fungicidal composition consists of (a) isofetamid and mandestrobin as active ingredients and (b) a solid or liquid carrier, a surfactant, or other formulating adjuvant, and wherein the disease is caused by harmful pathogenic fungus, and wherein the weight ratio of isofetamid to mandestrobin is 1:1000 to 1000:1.

3. The method according to claim 2, wherein the concentration of isofetamid to be applied is 0.01 ppm to 10000 ppm and the concentration of mandestrobin to be applied is 0.01 ppm to 10000 ppm.

4. A method for controlling a disease of a crop, which consists of applying a fungicidal composition consisting of a synergistic disease controlling amount of isofetamid and mandestrobin as active ingredients to the crop; wherein the fungicidal composition consists of (a) isofetamid and mandestrobin as active ingredients and (b) a solid or liquid carrier, a surfactant, or other formulating adjuvant, and wherein the disease is caused by a harmful pathogenic fungus, wherein the weight ratio of isofetamid to mandestrobin is 1:1000 to 1000:1, and wherein the concentration of isofetamid to be applied is 0.01 ppm to 10000 ppm, and wherein the concentration of mandestrobin to be applied is 0.01 ppm to 10000 ppm.

5. The method according to claim 4, wherein the weight ratio of isofetamid to mandestrobin is 1:10 to 10:1.

6. The method according to claim 2, wherein the harmful pathogenic fungus is selected from the group consisting of Venturia nashicola, Colletotrichum gloeosporioides and Colletotrichum acutatum.

7. The method according to claim 4, wherein the harmful pathogenic fungus is selected from the group consisting of Venturia nashicola, Colletotrichum gloeosporioides and Colletotrichum acutatum.

Description

EXAMPLES

(1) Hereinafter, Test Examples relating to the present invention are described but are not intended to limit the present invention.

Test Example 1: Effect Test Against Pear Scab (Japanese Pear Scab) (Effect on Leaves)

(2) A given amount of a chemical was sprayed on adult trees of pear (cultivar: Housui) with a pressurized sprayer in an amount sufficient to drip from the leaves. The spraying was performed four times in total at intervals of 8 to 10 days, about 50 leaves per area were investigated about the presence or absence of the onset of the disease days after the final spraying, and the ratio of the leaves with the onset of the disease and the disease control value were calculated (in triplicate). In addition, the theoretical value (of the disease control value) by mixing was also calculated according to the Colby's formula.
Disease control value=(1−a/b)×100
wherein
a: Mean ratio of leaves with the onset of the disease in the treated area,
b: Mean ratio of leaves with the onset of the disease in the untreated area;
Colby's formula=(X+Y)−XY/100
wherein
X: Disease control value when using isofetamid alone,
Y: Disease control value when using mandestrobin alone.

(3) When the actually measured value is higher than the theoretical value, the fungicide composition of the present invention has a synergistic effect on pear scab (Japanese pear scab).

(4) TABLE-US-00001 TABLE 1 Ratio of leaves with Disease Treating chemical, onset of control Theoretical concentration disease (%) value value Isofetamid, 190 ppm 40.5 56.4 Isofetamid, 90 ppm 31.3 66.3 Mandestrobin, 140 ppm 23.3 74.9 mandestrobin, 70 ppm 31.7 65.9 Isofetamid + 3.3 96.4 89.0 Mandestrobin, 190 ppm + 140 ppm Isofetamid + 2.7 97.1 88.5 Mandestrobin, 90 ppm + 70 ppm No treatment area 92.9

Test Example 2: Effect Test Against Pear Scab (Japanese Pear Scab) (Effect on Fruits)

(5) A given amount of a chemical was sprayed on adult trees of pear (cultivar: Housui) with a pressurized sprayer in an amount sufficient to drip from the leaves and fruits. The spraying was performed four times in total at intervals of 6 to 11 days, about 50 fruits per area were investigated about the presence or absence of the onset of the disease 8 days after the final spraying, and the ratio of the fruits with the onset of the disease and the disease control value were calculated (in triplicate). In addition, the theoretical value (of the disease control value) by mixing was also calculated according to the Colby's formula.
Disease control value=(1−a/b)×100
wherein
a: Mean ratio of fruits with the onset of the disease in the treated area,
b: Mean ratio of fruits with the onset of the disease in the untreated area.

(6) TABLE-US-00002 TABLE 2 Ratio of fruits with Disease Treating chemical, onset of control Theoretical concentration disease (%) value value Isofetamid, 187 ppm 17.2 62.3 Isofetamid, 134 ppm 10.3 77.5 Isofetamid, 93 ppm 7.6 83.4 Mmandestrobin, 140 ppm 3.0 93.4 Mandestrobin, 100 ppm 4.5 90.1 Mandestrobin, 70 ppm 10.3 77.5 Isofetamid + 0 100 97.5 Mandestrobin, 187 ppm + 140 ppm Isofetamid + 0 100 97.8 Mndestrobin, 134 ppm + 100 ppm Isofetamid + 0 100 96.3 Mandestrobin, 93 ppm + 70 ppm No treatment area 45.7

Test Example 3: Inhibitory Effect Test for Hyphal Elongation (Pear Scab (Japanese Pear Scab) Fungus, Venturia nashicola)

(7) A fungal flora (diameter: 4 mm) obtained by pre-cultivation was transplanted on PDA (potato dextrose agar medium) containing a given concentration of a chemical and then cultured at room temperature of 20° C. for 25 days, followed by measuring the diameter (mm) of the grown flora to determine the hyphal elongation inhibition rate.

(8) In addition, theoretical values (of the hyphal elongation inhibition rate) were calculated according to the Colby's calculation formula based on the above determined hyphal elongation inhibition rate, and presented in parenthesis in the table.
Inhibition rate=(1−a/b)×100
wherein
a: “Mean diameter (mm) of flora in the treated area”—4,
b: “Mean diameter (mm) of flora in the untreated area”—4
Colby's formula=(X+Y)−XY/100
X: Inhibition rate when using isofetamid alone
Y: Inhibition rate when using mandestrobin alone.

(9) When the experimental value is higher than the theoretical value, the fungicide composition of the present invention has a synergistic effect on pear scab (Japanese pear scab) fungus.

(10) TABLE-US-00003 TABLE 3 Mandestrobin Isofetamid 10 ppm 1 ppm 0.1 ppm 0.01 ppm 0 ppm 10 ppm 100 100 100 100 100 (100) (100) (100) (100) 1 ppm 100 81.6 56.7 56.0 49.6 (72.1) (63.6) (56.1) (49.6) 0.1 ppm 80.9 54.6 31.2 24.1 8.5 (49.4) (33.8) (20.2) (8.5) 0.01 ppm 64.5 34.0 19.9 7.8 0.0 (44.7) (27.7) (12.8) (0.0) 0 ppm 44.7 27.7 12.8 0.0 Numbers in the table: Inhibition rate (%), theoretical values in parentheses

Test Example 4: Inhibitory Effect Test for Hyphal Elongation (Apple Anthracnose (Apple Bitter Rot) Fungus, Colletotrichum gloeosporioides)

(11) A fungal flora (diameter: 4 mm) obtained by pre-cultivation was transplanted on PDA containing a given concentration of a chemical and then cultured at room temperature of 20° C. for 2 days, followed by measuring the diameter of the grown flora in the same manner as described in Test Example 3 to determine the hyphal elongation inhibition rate.

(12) In addition, theoretical values (of the hyphal elongation inhibition rate) were calculated according to the Colby's calculation formula based on the above determined hyphal elongation inhibition rate, and presented in parenthesis in the table.

(13) TABLE-US-00004 TABLE 4 Mandestrobin Isofetamid 10 ppm 1 ppm 0.1 ppm 0.01 ppm 0 ppm 10 ppm 51.9 49.4 32.7 38.5 2.6 (35.0) (16.3) (6.3) (4.4) 1 ppm 48.7 57.7 31.4 51.3 0.0 (33.3) (14.1) (3.8) (1.9) 0.1 ppm 62.2 36.5 20.5 0.6 0.0 (33.3) (14.1) (3.8) (1.9) 0.01 ppm 48.1 52.6 30.1 0.0 0.0 (33.3) (14.1) (3.8) (1.9) 0 ppm 33.3 14.1 3.8 1.9 Numbers in the table: Inhibition rate (%), theoretical values in parentheses

Test Example 5: Inhibitory Effect Test for Hyphal Elongation (Apple Anthracnose (Apple Bitter Rot) Fungus, Colletotrichum acutatum)

(14) A fungal flora (diameter: 4 mm) obtained by pre-cultivation was transplanted on PDA containing a given concentration of a chemical and then cultured at room temperature of 20° C. for 2 days, followed by measuring the diameter of the grown flora in the same manner as described in Test Example 3 to determine the hyphal elongation, inhibition rate.

(15) In addition, theoretical values (of the hyphal elongation inhibition rate) were calculated according to the Colby's calculation formula based on the above determined hyphal elongation inhibition rate, and presented in parenthesis in the table.

(16) TABLE-US-00005 TABLE 5 Mandestrobin Isofetamid 10 ppm 1 ppm 0.1 ppm 0.01 ppm 0 ppm 10 ppm 82.2 71.3 52.5 45.5 24.8 (66.5) (56.8) (24.8) (24.8) 1 ppm 82.2 59.4 38.6 59.4 0.0 (55.4) (42.6) (0.0) (0.0) 0.1 ppm 90.1 54.5 39.6 11.9 3.0 (56.8) (44.3) (3.0) (3.0) 0.01 ppm 86.1 57.4 37.6 21.8 6.9 (58.5) (46.6) (6.9) (6.9) 0 ppm 55.4 42.6 0.0 0.0 Numbers in the table: Inhibition rate (%), theoretical values in parentheses

(17) Now, Formulation Examples of the present invention will be described below.

(18) However, the blend ratio, the type of formulation or the like in the present invention is by no means restricted to the following Examples.

Formulation Example 1

(19) TABLE-US-00006 (1) Isofetamid 40 parts by weight (2) Mandestrobin 30 parts by weight (3) Sodium alkylbenzene sulfonate 14.5 parts by weight (4) NaCl 15 parts by weight (5) Dextrin 0.5 parts by weight

(20) The above components are added into a high-speed mixing granulator, mixed with an appropriate amount of water, granulated, and dried to obtain a water-dispersible granule.

Formulation Example 2

(21) TABLE-US-00007 (1) Kaolin 78 parts by weight (2) Sodium alkylnaphthalene sulfonate 2 parts by weight (3) Sodium polycarboxylate 5 parts by weight (4) Silica 15 parts by weight

(22) The mixture of the above components and the mixture of Isofetamid and Mandestrobin at a ratio of 9:7 are mixed in a weight ratio of 9:1 to obtain a wettable powder.

Formulation Example 3

(23) TABLE-US-00008 (1) Clay 33 parts by weight (2) Sodium naphthalenesulfonate 3 parts by weight (3) Sodium lignosulfonate 4 parts by weight (4) Isofetamid 30 parts by weight (5) Mandestrobin 30 parts by weight

(24) The above components are mixed to obtain a wettable powder.

Formulation Example 4

(25) TABLE-US-00009 (1) Isofetamid 4.5 parts by weight (2) Mandestrobin 3.5 parts by weight (3) Bentonite 30 parts by weight (4) Calcium carbonate 57.5 parts by weight (5) Sodium polyacrylate 3 parts by weight (6) Calcium lignosulfonate 1.5 parts by weight

(26) The above components (1) and (2) are mixed and pulverized. The components (3) and (4) are mixed with the mixture, then components (5), (6) and an appropriate amount of water are mixed thereto. The mixture is extruded and granulated, followed by drying and sieving to obtain granules.

Formulation Example 5

(27) TABLE-US-00010 (1) Isofetamid 20 parts by weight (2) Mandestrobin 15 parts by weight (3) Clay 55 parts by weight (4) Alkyl sodium sulfosuccinate 5 parts by weight (5) Alkylnaphtalenesulfonate condensed 5 parts by weight with formaldehyde

(28) The above components are mixed and pulverized. An appropriate amount of water is added to the mixture. The mixture is kneaded and then extruded and granulated, followed by drying and sieving to obtain a water-dispersible granule.

Formulation Example 6

(29) TABLE-US-00011 (1) Isofetamid 9 parts by weight (2) Mandestrobin 7 parts by weight (3) Polyoxyethylene tristyryl phenyl ether 3 parts by weight (4) Sodium polycarboxylate 2 parts by weight (5) Propylene glycol 10 parts by weight (6) Xanthangum 0.2 parts by weight (7) Preservative 0.1 parts by weight (8) Water 68.7 parts by weight

(30) The above components (1) to (4) and (8) are mixed and pulverized by a wet-grinding machine. Then components (5) to (7) are mixed with the mixture to obtain a water-based suspension concentrate.

Formulation Example 7

(31) TABLE-US-00012 (1) Isofetamid 20 parts by weight (2) Mandestrobin 15 parts by weight (3) Polyoxyethylene tristyryl phenyl ether 3 parts by weight (4) Dibutyl block copolymer 2 parts by weight (5) Propylene glycol 10 parts by weight (6) Xanthangum 0.1 parts by weight (7) Preservative 0.1 parts by weight (8) Water 49.8 parts by weight

(32) The above components (1) to (4) and (8) are mixed and pulverized by a wet-grinding machine. Then components (5) to (7) are mixed with the mixture to obtain a water-based suspension concentrate.

Formulation Example 8

(33) TABLE-US-00013 (1) Isofetamid 20 parts by weight (2) Mandestrobin 20 parts by weight (3) Polyoxyethylene tristyryl phenyl ether 3 parts by weight (4) Alkylnaphtalenesulfonate condensed 2 parts by weight with formaldehyde (5) Propylene glycol 10 parts by weight (6) Xanthangum 0.1 parts by weight (7) Preservative 0.1 parts by weight (8) Water 44.8 parts by weight

(34) The above components (1) to (4) and (8) are mixed and pulverized by a wet-grinding machine. Then components (5) to (7) are mixed with the mixture to obtain a water-based suspension concentrate.

Formulation Example 9

(35) TABLE-US-00014 (1) Isofetamid 30 parts by weight (2) Mandestrobin 20 parts by weight (3) Polyoxyethylene tristyryl phenyl ether 3 parts by weight (4) Alkyl sodium sulfosuccinate 2 parts by weight (5) Propylene glycol 10 parts by weight (6) Xanthangum 0.1 parts by weight (7) Preservative 0.1 parts by weight (8) Water 34.8 parts by weight

(36) The above components (1) to (4) and (8) are mixed and pulverized by a wet-grinding machine. Then components (5) to (7) are mixed with the mixture to obtain a water-based suspension concentrate.

Formulation Example 10

(37) TABLE-US-00015 (1) Isofetamid 30 parts by weight (2) Mandestrobin 20 parts by weight (3) Polyoxyethylene tristyryl phenyl ether sulfate 3 parts by weight (4) Alkyl sodium sulfosuccinate 2 parts by weight (5) Propylene glycol 10 parts by weight (6) Xanthangum 0.1 parts by weight (7) Preservative 0.1 parts by weight (8) Water 34.8 parts by weight

(38) The above components (1) to (4) and (8) are mixed and pulverized by a wet-grinding machine. Then components (5) to (7) are mixed with the mixture to obtain a water-based suspension concentrate.

Formulation Example 11

(39) TABLE-US-00016 (1) Isofetamid 10 parts by weight (2) Mandestrobin 10 parts by weight (3) Polyoxyethylene hydrogenated castor oil 12 parts by weight (4) Bentonite 1 parts by weight (5) Corn oil 67 parts by weight

(40) The above components are mixed and pulverized by a wet-grinding machine to obtain an oil-based dispersion.

Formulation Example 12

(41) TABLE-US-00017 (1) Isofetamid 9 parts by weight (2) Mandestrobin 7 parts by weight (3) Polyoxyethylene arylphenyl ether 10 parts by weight (4) Aromatic hydrocarbons 74 parts by weight

(42) The above components are mixed to obtain an emulsifiable concentrate.