Implant or medical tool made of a metal

Abstract

The invention relates to an implant or medical tool made of a metal or having a surface made of a metal for use in a therapeutic treatment, wherein the implant or the tool has, on its/the surface, a coating with polycrystalline doped electrically conductive diamond, wherein the therapeutic therapy is a treatment of a microbial infection of a human or animal body, wherein the implant or the tool is connected as anode (12) in an electrochemical system in the body, wherein the electrochemical system comprises, in addition to the anode (12), a cathode (16), a power source connected in an electrically conductive manner to the anode and to the cathode, and an electrolyte comprising or consisting of a body fluid, or consists of the anode (12), a cathode (16), a power source connected in an electrically conductive manner to the anode and to the cathode, and an electrolyte comprising or consisting of a body fluid, or wherein the implant or the tool is disposed within an electrical field, by means of which a negative charge is induced at a first site and a positive charge at a second site by induction on the implant or tool, by means of which the first site becomes the anode (12) in an electrochemical system and the second site becomes the cathode (16) in the electrochemical system, wherein the electrochemical system comprises, in addition to the implant or the tool, an electrolyte comprising or consisting of a body fluid or consists of the implant or the tool and an electrolyte comprising or consisting of a body fluid.

Claims

1. A therapeutic method, comprising treating microbial infection caused by bacteria on an implant in a human or animal body with oxidizing products having antimicrobial action that have been produced on an implant made of a metal or having a surface made of a metal, wherein the implant has, on its/the surface, a coating with polycrystalline doped electrically conductive diamond, wherein the therapeutic treatment comprises the generation of the products having antimicrobial action directly on the implant, wherein the products having antimicrobial action are C.sub.2O.sub.6.sup.2−, S.sub.2O.sub.8.sup.2− and/or P.sub.2O.sub.8.sup.4− ions, wherein the implant is connected as anode in an electrochemical system in the body, wherein the electrochemical system comprises, in addition to the anode, a cathode, a power source connected in an electrically conductive manner to the anode and to the cathode, and an electrolyte comprising or consisting of a body fluid, or consists of the anode, a cathode, a power source connected in an electrically conductive manner to the anode and to the cathode, and an electrolyte comprising or consisting of a body fluid, or wherein the implant is disposed within an electrical field, by means of which a negative charge is induced at a first site and a positive charge at a second site by induction on the implant, by means of which the first site becomes the anode in an electrochemical system and the second site becomes the cathode in the electrochemical system, wherein the electrochemical system comprises, in addition to the implant, an electrolyte comprising or consisting of a body fluid or consists of the implant and an electrolyte comprising or consisting of a body fluid, wherein an anodic electrode potential in the range of 1.80 V to 2.07 V is applied to the electrochemical system in order to generate the C.sub.2O.sub.8.sup.2−, S.sub.2O.sub.8.sup.2− and/or P.sub.2O.sub.8.sup.4− ions directly on the implant.

2. The method of claim 1, wherein the diamond has been doped with boron or phosphorus.

3. The method of claim 1, wherein the microbial infection is a bacterial infection or a fungal infection.

4. The method of claim 1, wherein the metal is titanium or a titanium-containing alloy.

5. The method of claim 4, wherein the alloy is Ti-6Al-4V, Ti-6Al-7Nb or another alloy containing, in addition to titanium, aluminum and/or niobium and/or iron and/or molybdenum.

6. The method of claim 1, wherein the coating is a coating produced on the surface of the implant in a gas phase containing a boron containing compound by hot filament chemical vapor deposition (HFCVD) or by microwave plasma-assisted chemical vapor deposition (MPCVD) or by another kind of chemical vapor deposition (CVD).

7. The method of claim 1, wherein the metal is titanium or the titanium-containing alloy and there is an interlayer of titanium carbide between the titanium or the alloy and the coating.

8. The method of claim 7, wherein the interlayer has a layer thickness of not more than 3 μm.

9. The method of claim 7, wherein the interlayer has a layer thickness of at least 100 nm.

10. The method of claim 8, wherein the interlayer has a layer thickness of at least 100 nm.

11. The method of claim 1, wherein the implant is a dental implant or jaw implant which is accessible from outside the body without surgical intervention and is electrically contacted for the therapy from outside the body, or wherein the implant has been implanted within the body and an electrical conductor, especially an electrically conductive wire, is routed from the implant to an outside of the body and is electrically contacted on the outside for the therapy or is routed from the implant to a contact site beneath the skin that is electrically contacted for the therapy from the outside through a puncture or is routed from the implant via an electrical rectifier to a coil, especially disposed not more than 3 cm beneath the skin, and is connected thereto in an electrically conductive manner, wherein the coil additionally has electrically conductive connection via the electrical rectifier to the cathode, wherein the cathode is likewise disposed within the body, wherein, for the therapy, a flow of current is induced in the coil by induction from outside the body, by means of which the cathode and the anode are energized.

12. The method of claim 1, wherein the electrical field is built up between at least two plates of a capacitor or by means of at least one electrical coil or is built up between a further anode and a further cathode within the electrochemical system, wherein the electrochemical system additionally comprises, in addition to the further anode and the further cathode, a power source connected in an electrically conductive manner to the further anode and the further cathode or additionally consists of the further anode and the further cathode and a power source connected in an electrically conductive manner to the further anode and the further cathode.

13. The method of claim 12, wherein the electrolyte in the electrochemical system comprises an electrically conductive auxiliary fluid or consists of the body fluid and the electrically conductive auxiliary fluid, wherein the electrically conductive auxiliary fluid in each case is a liquid that establishes electrical contact of the further anode and the further cathode with the body fluid.

14. The method of claim 1, wherein the implant is disposed within a closed region of the body, wherein a voltage applied to the electrochemical system is chosen such that the amount of gaseous further products formed at the anode is sufficiently low that these dissolve directly in the body fluid after they have formed.

15. The method of claim 1, wherein the microbial infection is a microbial infection associated with an inflammation reaction.

16. The method of claim 1, wherein the implant is an endoprosthesis made of a metal.

17. The method of claim 1, wherein the diamond is microdiamond.

18. The method of claim 17, wherein the microdiamond has a grain size ranging from 2 to 3 μm.

Description

(1) The invention is elucidated in detail hereinafter with reference to working examples. The figures show:

(2) FIG. 1 a current density-potential curve on boron-doped diamond layers,

(3) FIG. 2 an experimental setup shown in schematic form in cross section for inactivation of E. coli bacteria in an agar biofilm,

(4) FIG. 3. an alternative experimental setup shown in schematic form in cross section for inactivation of E. coli bacteria in an agar biofilm,

(5) FIG. 4. an experimental setup shown in schematic form in cross section for inactivation of S. gordonii bacteria initially adhered directly to a BDD anode.

(6) FIG. 5 a diagram to show the dependence of the number of E. coli colonies on space charge density at a constant voltage of 4.2 V,

(7) FIG. 6 a diagram to show the number of E. coli colonies as a function of space charge density and experimental setup,

(8) FIG. 7 a diagram to show the area colonized by S. gordonii on BDD-coated titanium platelets as a function of surface charge density and voltage applied,

(9) FIG. 8 an experimental setup shown in schematic form in cross section for inactivation of Staphylococcus epidermidis and Bacillus subtilis in a drilled root canal of a human tooth,

(10) FIG. 9 a diagram to show the dependence of the growth of Staphylococcus epidermidis on the amount of charge and the duration of treatment of the root canal and

(11) FIG. 10 a diagram to show the dependence of the growth of Bacillus subtilis on the amount of charge and the duration of treatment of the root canal.

EXPERIMENTAL

(12) CVD Diamond Coating of Titanium Platelets

(13) The substrate material used was pure titanium according to ASTM Standard F-67 Grade 4 and according to ISO Standard 5832-2 from L. Klein S A. The chemical composition is shown in table 2 below.

(14) TABLE-US-00002 TABLE 2 Chemical composition of Grade 4 titanium according to ASTM Standard F-67. C N O Fe H Ti 0.0050% 0.0035% 0.2650 0.0200% 0.0032% balance

(15) The sample wafers separated therefrom by means of an Accutom (from Struers) have a diameter of 12 mm and a height of 1.8 mm.

(16) A standard sample pretreatment was conducted, consisting of the steps of fine blasting, etching and seeding. The fine blasting was effected manually with silicon carbide (SiC) particles on a system from Wassermann. To establish different roughnesses, finer F320 (17 to 74 μm) SiC particles were used for a smoother titanium surface or coarser F80 (125 to 300 μm) SiC particles for a rougher titanium surface. The jet pressure was about 2.5 bar. This was followed by etching between 80° C. and 90° C. in aqueous solution of 10% H.sub.2SO.sub.4 and 10% HCl for 10 min. This increases the micro-roughness of the substrate surface. In the last step, the samples were seeded with a dilution of the 5% by weight aqueous nanodiamond particle solution from Carbodeon in an ultrasound bath for 5 min. This increases the seed density on the substrate surface as a prerequisite for a continuous diamond layer. For this purpose, the “ANDANTE” diamond suspension from Carbodeon Ltd. Oy, Pakkalankuja 5, 01510 Vantaa, Finland was used in a ratio of 1:1000 with ethanol.

(17) The coating and filament insertion processes were conducted with the CemeCon Hot Filament CVD (HFCVD) CC800 Dia-8 system. The copper filament holders were disposed in rails 1 and 2 in both processes and were each strung with 42 tungsten wires having a length of 220 mm. The filament material used was AKS-doped tungsten wire with diameter 0.11 mm.

(18) The insertion processes took place at a starting pressure of 6500 mPa, process pressure 6 mbar, a methane flow of 16 min and hydrogen flow 1000 min over a period of 18 h. A two-channel pyrometer placed in front of the viewing window of the closed HFCVD system was used to monitor the filament temperature. 50 titanium platelets were coated with microdiamond and 30 titanium platelets with nanodiamond. In all HFCVD processes, the starting pressure was 6000 mPa and the process pressure 6 mbar. The sample temperature of 820° C. and the filament temperature of 2160° C. were established by closed-loop control. Each coating process consists of three segments with different gas flows. The difference between micro- and nano-coating processes lies in the methane content, which is higher in the case of nano-coating. Table 3 below shows the process parameters of the diamond coatings.

(19) TABLE-US-00003 TABLE 3 Process parameters and properties of the micro- and nanodiamond coatings. Process CH.sub.4 TMB Layer Process duration H.sub.2 flow content content Current thickness number [h] [mln] [%] [mln] [A] [μm] K518  1.5/  3000/ 1.6  0.27/  80/ 2.5 (F80)/ Mikro 10.5/  2000/  0.18/  82/ 1.5 F80/F320 0.02/ 1000 0.00 15 (F320) K561  1.5/  3000/ 1.6  0.27/  60/ 8.2 Mikro 12.5/  2000/  0.18/  86/ F320 0.02 1000 0.00 15 K571  1.5/  3000/ 1.6  0.27/  60/ 6.1 Mikro 18.5/  2000/  0.18/  80/ F320 0.02 1000 0.00 15 K553  1.5/  3000/  3.0/  0.27/  80/ 0.8 Nano 12.5/  2000/  3.5/  0.18/  84/ F320 0.02 1000 1.6 0.00 15 K575  1.5/  3000/  3.0/  0.27/  80/ 3.7 Nano 18.5/  2000/  3.5/  0.18/  88/ F320 0.02 1000 1.6 0.00 15
Inactivation of Escherichia coli
Production of an E. coli Agar Biofilm

(20) Synthetic biofilms were produced by adding nonpathogenic Escherichia coli-K12 498 (E. coli) from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ) from a preculture in liquid standard 1 growth medium (St. 1). St. 1 with agar-agar is referred to hereinafter as St. 1 agar. The constituents of this St. 1 agar are listed in table 4 below.

(21) TABLE-US-00004 TABLE 4 Ingredients of the standard 1 agar growth medium in 400 ml of demineralized water. Amount Manufacturer/article Ingredients [g] number Glucose monohydrate 0.44 Carl Roth GmbH/6780.1 Sodium chloride 2.34 Carl Roth GmbH/3957.1 Agar-agar 8.00 Carl Roth GmbH/5210.3 Yeast extract 1.20 Carl Roth GmbH/2363.3 Peptone from casein 6.00 Merck KGaA/1.02239.0500

(22) The pH was adjusted to 7.4 by means of addition of sodium hydroxide. Plated out in petri dishes, the St. 1 agar solidified after a few minutes and served as culture medium. 10 ml of St. 1 agar was introduced into a centrifuge tube and kept in liquid form in a water bath at 50° C.

(23) 1000 E. coli bacteria were introduced into the 10 ml of liquid (50° C.) St. 1 agar and, after homogenizing, pipetted into autoclaved plastic rings of different height according to table 5 below.

(24) TABLE-US-00005 TABLE 5 Amount of St. 1 agar with E. coli per plastic ring. Ring height Amount of St. 1 agar [mm] with E. coli [μl] 0.5  50 1.0  90 2.0 150 3.0 200
Test Setup for Inactivation of E. coli in Agar Biofilm

(25) The resulting biofilm having a diameter of 8 mm was between a microdiamond-coated anode 12 of titanium and a stainless steel sheet as cathode 16 during the experiment. A titanium rod 10 was used for contacting of the anode 12 and cathode 16 in that it was pressed manually on to the coated anode 12 or the cathode 16. In the experimental setup shown in FIG. 2, the titanium rod 10 is connected to the plus pole and the stainless steel sheet to the minus pole of a power source. Beneath a sterile safety cabinet, voltages of 4.0 V, 4.2 V and 4.5 V were applied for between 1 min and 6 min and the respective corresponding current flow was measured.

(26) In order to rule out diffusion of the hydrogen gas formed at the cathode from below through the biofilm in the upward direction and hence inactivation of E. coli by hydrogen, anode 12 and cathode 16 were exchanged in some experiments. The corresponding experimental setup is shown in FIG. 3.

(27) Quantification of the Bacterial Colonies

(28) Subsequently, the treated biofilms were separated from the rings and they were positioned on solid St. 1 agar culture media in petri dishes. They were incubated at 27° C. for about 7 days.

(29) The colonies formed within the biofilms were apparent as dark spots by the naked eye and were counted under 40-fold magnification under a light microscope.

(30) Inactivation of Streptococcus gordonii

(31) Initial Adhesion of Streptococcus gordonii

(32) The BDD-coated titanium platelets were initially bacterially colonized. The culture medium used in a preculture was autoclaved Tryptone Soya Broth (TSB) to which yeast extract had been additionally added. The constituents of the culture medium are listed in table 6 below.

(33) TABLE-US-00006 TABLE 6 Ingredients of the Tryptone Soya Broth culture medium with yeast extract in 1 l of tridistilled water. Trade name Amount Manufacturer/Art. No. Tryptone Soya Broth 30.0 g Oxoid LTD Yeast extract  3.0 g Carl Roth GmbH/2363.3

(34) 50 ml of the culture medium was inoculated with 50 μl of the oral bacterium S. gordonii DSMZ 20568 in an Erlenmeyer flask and aerobically incubated at 37° C. while stirring for 18 h. Subsequently, the resulting culture was transferred to a 50 ml centrifuge tube and centrifuged at 4° C. for 15 min and 4000 g. The supernatant was poured off and the pellet was resuspended in 50 ml of a 50 mM tris(hydroxymethyl)aminomethane solution that had been adjusted to pH 7.5 by means of hydrochloric acid (HCl) (TRIS HCl). Subsequently, 25 ml of TRIS HCl was made up to 500 ml with tridistilled water. The steps of centrifuging and resuspending were repeated twice more. In the last resuspension, only 20 ml of the 50 mM TRIS buffer was used, and this was followed by vortexing for 5 min. By means of the buffer solution used, the resulting bacterial suspension was adjusted to an optical density (OD) of 0.7 at a wavelength of 600 nm. Each of the coated titanium platelets was placed with its coating upward into one of the wells of a 6-well plate from Greiner Bio-One. 3 ml of the bacterial suspension was placed onto each of the coated titanium platelets.

(35) Initial adhesion took place at 37° C. in an incubator at ventilation level 3 with slight rotation at 150 revolutions per minute within 5 h. The colonized platelets were washed twice with 3 ml each time of Dulbecco's phosphate-buffered saline (PBS). Each well was filled to the brim with PBS for the experiment.

(36) Experimental Setup for Inactivation of Initially Adhered Streptococcus Gordonii

(37) The experimental setup is shown in FIG. 4. At the base of each of the PBS-filled wells 22 were the BDD-coated and bacterially colonized titanium platelets that were connected as anode 12 in the electrochemical experiment. A titanium-aluminum rod 18 of diameter 2 mm made contact with the anode 12. The cathode 16 made of stainless steel with diameter 22 mm was in a round recess in the PVC lid 20 that was fitted to the diameter of the well 22 of 40 mm. The titanium-aluminum rod 18 was guided through a hole in the cathode 16 to make contact with the anode 12. Teflon served as insulation material between the two electrodes. The titanium-aluminum rod 18 was connected to the plus pole, while the cathode 16 was attached to the minus pole of a power source. A series-connected multimeter detected the current flow with voltage applied. Applying the voltage gave rise to an electrical field 24 between the anode 12 and the cathode 16.

(38) The stimulation was followed by a washing operation with 3 ml of PBS in order to remove the detached bacteria. The live/dead distribution on the platelets was determined using a live/dead stain of the adhered streptococci with fluorescent dyes. For this purpose, the green live dye SYTO9 and the red dead dye propidium iodide from the Live/Dead BacLight Bacterial Viability Kit from Thermofisher Scientific were used. 3 ml of a staining solution in which each of the two dyes was diluted 1:1000 with PBS wetted all the platelets. Since both dyes are light-sensitive, the staining process took place under darkened conditions. The contact time was about 30 min. The live dye binds to the DNA of all bacterial cells, while the dead dye penetrates solely into bacteria with a destroyed membrane and displaces the live dye. Thereafter, the staining solution was replaced by the same amount of 2.5% glutaraldehyde as fixative. This is a highly reactive acidic solution that crosslinks proteins in the bacterial membrane by reaction with amino groups and hence kills the bacterial cells. Before the microscope characterization, after a contact time of at least 15 min, the fixing solution was exchanged for PBS.

(39) In the first experiments, voltages between 1.5 V and 3.5 V were applied for 2 min or 3 min. These results were used to calculate the charge density needed to kill the adhered bacteria, and this was established in a controlled manner in subsequent experiments. The surface charge densities here were from 18 (A*s)/m.sup.2 to 18 000 (A*s)/m.sup.2.

(40) Every experiment in which charge densities were set was conducted three times in independent biological replicates. Comparisons took place between micro- and nanodiamond coating, and between diamond coating and straight titanium platelets.

(41) Inactivation of Human Gingival Fibroblasts

(42) In order to test the influence of the bacterial inactivation by means of free-radical formation on BDD-coated implant surfaces on the surrounding tissue, an experiment with six BDD-coated titanium platelets was used, one of which served as untreated control.

(43) The tissue cells used were human gingival fibroblasts from the gum. These primary cells have been isolated from tissue that was still intact immediately beforehand. The preculture grew within three days in DMEM cell culture medium (from Merck Millipore) with the ingredients apparent from table 7 below, 10% fetal calf serum and 1% penicillin.

(44) TABLE-US-00007 TABLE 7 Ingredients of the DMEM cell culture growth medium in 0.5 l of tridistilled water. Trade name Amount Sodium hydrogencarbonate 3.7 g/l D-Glucose 4.5 g/l L-Glutamine 0.6 g/l

(45) 50 000 cells in 120 μl of a cell suspension were sown on each of the BDD-coated titanium platelets. The cells here were in the eighth passage. The platelets were incubated at 37° C. and 5% CO.sub.2 for 2 h. The pH of the cell culture medium here was about 7.7. After the adhesion of the fibroblasts, each of the six platelets in the wells 22 were wetted with 3 ml of DMEM cell culture medium and incubated for 24 h. This was followed by treatment in PBS with the experimental setup shown in FIG. 4 and the same charge densities as in the case of initial adhesion and biofilm from S. gordonii. Thereafter, replacement of the PBS with 3 ml per well of the staining solution took place. Each of the two dyes has been diluted 1:1000 therein with PBS. The live dye used was calcein from Invitrogen, the dead dye propidium iodide from SIGMA. The experiment was evaluated using five photographs per platelet with the Zeiss Axio Scope Al microscope in 200-fold magnification.

(46) Results and Discussion

(47) All results are plotted against electrical charge density as a measure of the bactericidal oxidizing agents formed. This was either specifically established in the experiment or calculated subsequently. For this purpose, it was necessary to know the current measured at the voltage applied, the corresponding treatment time and the volume of the biofilm or the area of adhered bacteria.

(48) The charge distribution in a volume is referred to as space charge density e.

(49) $\left[\varrho \right]=1\frac{\mathrm{As}}{m^3}$

(50) The uniform distribution of the charge over any area is referred to as surface charge density σ.

(51) $\left[\sigma \right]=1\frac{\mathrm{As}}{m^2}$
Inactivation of Escherichia coli in an Agar Biofilm
E. coli Inactivation in Different Biofilm Volumes

(52) The diagram in FIG. 5 illustrates an experiment in which each biofilm was subjected to a load of 4.2 V for 2 min with the cathode 16 positioned at the top according to the experimental setup shown in FIG. 3. Each point on the graph is the averaged number of colonies from three sites in a biofilm. It is clearly apparent that, with this constant voltage and treatment time, higher space charge densities occur in thinner biofilms than in the thicker biofilms and hence more colonies are inactivated there. Within identical biofilm heights, owing to different contact resistances and associated varying current intensities, different space charge densities arise. In the biofilms of height 2 mm and 3 mm, there is barely any difference in the space charge densities and the number of colonies, while the thinner biofilms include far fewer colonies. Complete elimination takes place from about 10 (A*s)/cm.sup.3. The volume of the 0.5 mm biofilms corresponds to 0.025 cm.sup.3, the volume of the 1 mm biofilms to 0.05 cm.sup.3, the volume of the 2 mm biofilms to 0.1 cm.sup.3, and the volume of the 3 mm biofilms to 0.16 cm.sup.3.

(53) FIG. 5 shows the dependence of the number of E. coli colonies on space charge density at a constant voltage of 4.2 V for various biofilm volumes. Overall, the number of bacterial colonies drops with increasing space charge density. In the thinner biofilms, the bacteria are more intensely inactivated since higher space charge densities are attained therein.

(54) Comparison of Two Different Arrangements of the Cathode

(55) In order to check whether the evolution of hydrogen at the cathode enhances bacterial inactivation by hydroxyl radicals and other oxidizing agents, the position of the cathode was varied, as illustrated in FIG. 2 and FIG. 3.

(56) It was expected that atomic hydrogen with the cathode at the bottom would diffuse up through the biofilm and possibly be able to kill bacteria. However, the results in this case show lower inactivation of E. coli as a result of inadequate space charge densities. If the construction is reversed and the cathode positioned at the top, contact resistances are lower, and hence the space charge densities are higher and more bacteria die. This is shown in FIG. 6 in the case of an experiment with 4.2 V and 1 mm-thick agar biofilms.

(57) FIG. 6 shows the number of E. coli colonies as a function of space charge density. This involved comparing the experimental setup according to FIG. 2 and FIG. 3 for inactivation of E. coli at 4.2 V in 1 mm-thick agar biofilms for 1 and 3 min. It was found that, with the cathode disposed at the bottom, no increased bacterial inactivation by the hydrogen formed took place.

(58) Summary of Inactivation of Escherichia coli in an Agar Biofilm

(59) In general, in all experiments with different biofilm volumes, it was found that more bacterial colonies were inactivated by higher space charge densities, and space charge density, by definition, was inversely proportional to biofilm height.

(60) It was found that E. coli can be completely inactivated by electrochemical treatment with diamond anodes. At 4.0 V and 4.2 V, the minimum charge density needed for the purpose in 1 mm-thick biofilms is 2 (A*s)/cm.sup.3 and 10 (A*s)/cm.sup.3 respectively for 2 min.

(61) It was not possible to demonstrate inactivation of E. coli by hydrogen formed at the cathode in the experimental setup according to FIG. 2.

(62) Elimination of E. coli by evolution of high temperature during the electrochemical experiment was likewise examined and was ruled out.

(63) Inactivation of Initially Adhered Streptococcus gordonii

(64) The results that follow relate to electrochemical experiments in a liquid electrolyte. The experimental setup is shown in FIG. 4.

(65) Owing to a very small height of the individually adhered S. gordonii and of the biofilms, which is less than 30 μm, the live/dead distribution in the diagrams here was based on the surface charge density.

(66) Determining of the Voltage for Bacterial Inactivation

(67) The voltage range in which an oxidizing agent-implemented disinfection of the BDD-coated surface of the titanium platelets colonized by bacterial adhesion with S. gordonii was to be observed was determined.

(68) FIG. 7 shows the average area per sample colonized by S. gordonii by initial adhesion to BDD-coated titanium platelets after application of various voltages as a function of the calculated surface charge density in (A*s)/m.sup.2 and the voltage applied. All five samples are treated for 2 min. No voltage was applied to the colonized control. The standard deviations show the variation of the five measurement points within a sample. Only few dead cells that have died off naturally are present on the untreated control. In the case of the titanium platelets treated at 1.5 V and 2.5 V, the dead fraction is comparable to that of the control. However, the live fraction in these samples is much lower than that of the control. It is likely that fewer microbes found survival conditions for colonization or the microbes were washed off more easily in the washing operation. A very high area fraction of dead bacteria is shown by the samples to which 3.0 V and 3.5 V were applied. Virtually all oral microbes were inactivated at these voltages.

(69) The comparison of different voltages on boron-doped microdiamond-coated platelets (K571) indicates that the transition from live to dead S. gordonii microbes takes place between 2.5 V and 3.0 V.

(70) Experiments with boron-doped micro- and nanodiamond coatings, which are not shown, did not show any crucial influence of the diamond structure on the electrochemical inactivation of S. gordonii. The morphology of diamond merely affects the number of adhered bacteria, but not the inactivated fraction thereof.

(71) Influence of the Oxidizing Products on Human Gingival Fibroblasts

(72) An experiment with human fibroblasts from the gum under the same conditions as in the experiments conducted with S. gordonii did not show any killing of the eukaryotic cells. Under treatment with the highest charge densities, exclusively green-stained live fibroblasts are apparent. It can be concluded from this that electrochemical surface disinfection is possible in principle in the oral cavity with voltages of up to 3.5 V and surface charge densities of 18 000 (A*s)/m.sup.2.

(73) Conclusion

(74) The inventors were able to show that a boron-doped diamond coating on an anode can be used to completely electrochemically inactivate both Gram-negative Escherichia coli bacteria and Gram-positive Streptococcus gordonii species. This means that BDD-coated implants make it possible to electrochemically prevent inflammation reactions and control infections.

(75) Electrochemical therapy of a microbial infection at a BDD-coated implant surface with comparatively low voltage and charge density is tolerated by the human or animal body without difficulty for the short treatment time required for the purpose. If required, the treatment can be repeated or stopped at any time.

(76) Since numerous oxidizing agents are formed directly at the diamond anode in the therapy, bacteria can be inactivated directly and effectively at the implant surface. Fibroblasts did not react negatively to this treatment in an experiment.

(77) Inactivation of Staphylococcus epidermidis and Bacillus subtilis in a Drilled Root Canal of a Human Tooth

(78) Extracted human teeth with a drilled root canal were obtained from a dentist. The teeth were first incubated for at least 20 hours in a physiological saline containing either Staphylococcus epidermidis or Bacillus subtilis. The root canals were colonized here with the respective bacteria. Subsequently, the teeth were rinsed with physiological saline and placed in physiological saline as electrolyte in the experimental setup shown in FIG. 8. The anode here consisted of a boron-doped diamond-coated niobium wire, and the cathode of steel. A voltage in the range from 5 to 9 volts was applied, such that the amounts of charge specified in the figures have flowed within the period of treatment specified in each case.

(79) After the period of treatment specified in each case, the respective tooth was split and the resultant inner split surfaces including the surface of the root canal were impressed repeatedly on an St.1 agar culture medium and finally stored with this surface on the agar. The agar culture medium was then incubated at 27 degrees for 1-2 days. Bacterial growth was apparent from the formation of colonies. The colonies were apparent to the naked eye as dots and were subjectively categorized into no, moderate, average and high bacterial growth.

(80) Inactivation of Staphylococcus epidermidis

(81) The results apparent from FIG. 9 show that complete sterilization of the inner root canal surface was achieved even with an amount of current of 4 As, corresponding to a treatment time of 3.5 minutes. No bacterial growth was found on any of the agar culture media.

(82) Inactivation of Bacillus subtilis

(83) The results apparent from FIG. 10 show that complete sterilization of the inner root canal surface was found even with an amount of current of 5 As, corresponding to a treatment time of 8.5 minutes. Moderate bacterial growth found after a treatment time of 42 minutes and with an amount of current of 25 As may have been caused by the ability of Bacillus subtilis to form spores and the possible presence of these spores in the dentinal tubules of the root canal. It was additionally found that, in the event of spore formation, the amount of charge required for complete sterilization can increase by more than one hundred times.

(84) Results and Discussion

(85) The experiments conducted on an extracted tooth show that a boron-doped diamond-coated anode is of good suitability for disinfection of a root canal with a comparatively short time. Such an anode, for example in the form of a boron-doped diamond-coated wire, can be introduced for the purpose into an open root canal and energized in a dental treatment. The cathode required for the purpose may be placed close to the tooth to be treated within the oral cavity. The coated wire to be introduced into the root canal is an implant for the purposes of the invention.

(86) In a dental treatment, it is also conceivable that a tool used for dental treatment, for example a drill, is coated with boron-doped diamond and is connected as anode in the treatment. Such a tool is then a surgical tool for the purposes of the invention.

LIST OF REFERENCE NUMERALS

(87) 10 titanium rod 12 anode 16 cathode 18 titanium-aluminum rod 20 lid 22 well 24 electrical field