BIOMOLECULAR CHARACTERISTIC IDENTIFICATION METHOD AND SYSTEM THEREOF FOR TAIWAN TILAPIA STRAINS

Abstract

A biomolecular characteristic identification method includes: breeding a plurality of Taiwan Tilapias in a predetermined environment to obtain a plurality of baited Taiwan Tilapias; seeking at least one nucleotide mark in the baited Taiwan Tilapias to obtain at least one feature sequence marker therefrom; producing at least one design of primer pair according to the feature sequence marker for identification; and identifying an unknown DNA sample of the baited Taiwan Tilapias with the at least one primer pair in a biomolecular tracing procedure to obtain an identification result.

Claims

1. A biomolecular characteristic identification system for Taiwan Tilapia strains comprising: a predetermined environment conditioned and provided to breed and train a plurality of Taiwan Tilapias to obtain a plurality of baited Taiwan Tilapias; at least one feature sequence marker obtained from seeking at least one nucleotide mark in the baited Taiwan Tilapias; at least one designed primer pair corresponding to the feature sequence marker for identification and produced according to the feature sequence marker; and an identification result obtained from identifying an unknown DNA sample of the baited Taiwan Tilapias with the at least one primer pair in a biomolecular tracing procedure; wherein the identification result shows a correct source of original baited Taiwan Tilapias or an incorrect source of original baited Taiwan Tilapias.

2. The biomolecular characteristic identification system as defined in claim 1, wherein the feature sequence marker is selected from a cold hardiness marker, a disease resistance marker, a salinity tolerance marker or combinations thereof.

3. The biomolecular characteristic identification system as defined in claim 2, wherein the cold hardiness marker is selected from a (ATT).sub.n-sequence-contained marker, a (G).sub.n-sequence-contained marker, a (GAG).sub.n-sequence-contained marker, a (GT).sub.n-sequence-contained marker or a (CA).sub.n-sequence-contained marker.

4. The biomolecular characteristic identification system as defined in claim 2, wherein the salinity tolerance marker is selected from a (CA).sub.11-sequence-contained marker, a (AT).sub.8-sequence-contained marker, a (TAT).sub.8-sequence-contained marker, a (TTTG).sub.5-sequence-contained marker or a (AT).sub.12-sequence-contained marker.

5. The biomolecular characteristic identification system as defined in claim 2, wherein the disease resistance marker is selected from a (CAGG).sub.7-sequence-contained marker, a (CTAC).sub.7-sequence-contained marker, a (TTGA).sub.20-sequence-contained marker, a (TTGT).sub.10-sequence-contained marker or a (GAAAA).sub.6-sequence-contained marker.

6. A biomolecular characteristic identification method for Taiwan Tilapia strains comprising: breeding and training a plurality of Taiwan Tilapias in a predetermined environment to obtain a plurality of baited Taiwan Tilapias; seeking at least one nucleotide mark in the baited Taiwan Tilapias to obtain at least one feature sequence marker therefrom; producing at least one design of primer pair according to the feature sequence marker for identification; and identifying an unknown DNA sample of the baited Taiwan Tilapias with the at least one primer pair in a biomolecular tracing procedure to obtain an identification result.

7. The biomolecular characteristic identification method as defined in claim 6, wherein the feature sequence marker is selected from a cold hardiness marker, a disease resistance marker, a salinity tolerance marker or combinations thereof.

8. The biomolecular characteristic identification method as defined in claim 7, wherein the cold hardiness marker is selected from a (ATT).sub.n-sequence-contained marker, a (G).sub.n-sequence-contained marker, a (GAG).sub.n-sequence-contained marker, a (GT).sub.n-sequence-contained marker or a (CA).sub.n-sequence-contained marker.

9. The biomolecular characteristic identification method as defined in claim 7, wherein the salinity tolerance marker is selected from a (CA).sub.n-sequence-contained marker, a (AT).sub.8-sequence-contained marker, a (TAT).sub.8-sequence-contained marker, a (TTTG).sub.5-sequence-contained marker or a (AT).sub.12-sequence-contained marker.

10. The biomolecular characteristic identification method as defined in claim 7, wherein the disease resistance marker is selected from a (CAGG).sub.7-sequence-contained marker, a (CTAC).sub.7-sequence-contained marker, a (TTGA).sub.20-sequence-contained marker, a (TTGT).sub.10-sequence-contained marker or a (GAAAA).sub.6-sequence-contained marker.

11. The biomolecular characteristic identification method as defined in claim 6, further comprising an exogenous biomolecular tracing method comprising: extracting a first nucleotide marker from a first marker source organism; combining a first basic material with the first nucleotide marker to form a first nucleotide marker-contained material; extracting a second nucleotide marker from a second marker source organism; combining a second basic material with the second nucleotide marker to form a second nucleotide marker-contained material; in a first stage, exogenously combining the first nucleotide marker-contained material with an aquatic creature or an aquatic product to form a first exogenously-marked aquatic creature or a first exogenously-marked aquatic product; in a second stage, exogenously combining the second nucleotide marker-contained material with the first exogenously-marked aquatic creature or the first exogenously-marked aquatic product to form a second exogenously-marked aquatic creature or a second exogenously-marked aquatic product; and identifying a DNA sample received from the second exogenously-marked aquatic creature or the second exogenously-marked aquatic product with at least one primer pair in an exogenous biomolecular tracing procedure to obtain an exogenously-marked identification result.

12. The method as defined in claim 11, wherein the first or second nucleotide marker is selected from a DNA extract, a DNA extract liquid, a DNA extract powder, a DNA extract-related material or combinations thereof.

13. The method as defined in claim 11, wherein exogenously combining the first or second nucleotide marker-contained material with the aquatic creature or the aquatic product includes a feeding process, a dipping process, a painting process, a spraying process or combinations thereof.

14. The method as defined in claim 11, wherein the at least one primer pair is applied to execute a test of polymerase chain reaction (PCR) or loop-mediated isothermal amplification (LAMP).

15. The method as defined in claim 11, wherein the exogenously-marked identification result includes a combination set of codes or code forms for tracing the second exogenously-marked aquatic creature or the second exogenously-marked aquatic product.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0045] The present invention will become more fully understood from the detailed description given hereinbelow and the accompanying drawings which are given by way of illustration only, and thus are not limitative of the present invention, and wherein:

[0046] FIG. 1 is a schematic block diagram of a biomolecular characteristic identification system for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention.

[0047] FIG. 2 is a schematic block diagram of a series of feature sequence markers of Taiwan Tilapia applied in the biomolecular characteristic identification method and system thereof for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention.

[0048] FIG. 3 is a flow chart of a biomolecular characteristic identification method for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention.

[0049] FIG. 4 is a schematic block diagram of an exogenous biomolecular tracing system applied in the biomolecular characteristic identification method and system thereof for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention.

[0050] FIG. 5 is a flow chart of an exogenous biomolecular tracing system applied in the biomolecular characteristic identification method and system thereof for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention.

DETAILED DESCRIPTION OF THE INVENTION

[0051] It is noted that a biomolecular characteristic identification method (or usage) and system (e.g., information system) thereof for Taiwan Tilapia strains in accordance with the preferred embodiment of the present invention can be applicable to various aquatic creature baiting systems, various aquatic fry or creature breeding systems, various aquatic creature display systems, various algae (or microalgae) cultivation systems or various algae display systems, which are not limitative of the present invention.

[0052] For instance, a biomolecular characteristic identification method (or usage) and system (e.g., information system) thereof for Taiwan Tilapia strains in accordance with the preferred embodiment of the present invention can be suitable for a Taiwan Tilapia strain (National Taiwan Ocean University), including Taiwan Tilapia (A), Taiwan Tilapia (Y), Taiwan Tilapia (B), Taiwan Tilapia (T) or other related Taiwan Tilapia strains, which are not limitative of the present invention.

[0053] FIG. 1 shows a schematic block diagram of a biomolecular characteristic identification system for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention. Referring now to FIG. 1, the biomolecular characteristic identification system in accordance with the preferred embodiment of the present invention includes a predetermined environment 100, at least one feature sequence marker 1a, a plurality of Taiwan Tilapia organism 2 and at least one primer pair 3a.

[0054] With continued reference to FIG. 1, by way of example, the predetermined environment 100 can be selected from a cage culture site, an aquaculture outdoor site, an aquaculture tank, an aquaculture container or the likes, and controlled by various conditions, including temperature parameters, salinity parameters, pathogenic bacteria parameters or other environment conditions for training Taiwan Tilapia.

[0055] Still referring to FIG. 1, by way of example, the Taiwan Tilapia organism 2 can be selected from predetermined strains of Taiwan Tilapia (A), Taiwan Tilapia (Y), Taiwan Tilapia (B), Taiwan Tilapia (T) or other similar strains.

[0056] With continued reference to FIG. 1, by way of example, in another embodiment, the biomolecular characteristic identification system further includes at least one extraction device 1, at least one DNA testing device 3 and related peripheral devices, e.g. feeding machine, painting machine, spraying machine or other machines.

[0057] FIG. 2 shows a schematic block diagram of a series of feature sequence markers of Taiwan Tilapia applied in the biomolecular characteristic identification method and system thereof for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention. Turning now to FIGS. 1 and 2, by way of example, the Taiwan Tilapia organism 2 has at least one feature sequence marker, including a cold hardiness marker, a disease resistance marker, a salinity tolerance marker or combinations thereof.

[0058] FIG. 3 shows a flow chart of a biomolecular characteristic identification method for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention. Turning now to FIGS. 1, 2 and 3, by way of example, the biomolecular characteristic identification method in accordance with the preferred embodiment of the present invention includes the step S1: automatically, semi-automatically or manually breeding and training a plurality of Taiwan Tilapias (e.g., Taiwan Tilapias organism 2) in the predetermined environment 100 with various conditions to obtain a plurality of baited Taiwan Tilapias.

[0059] Still referring to FIGS. 1, 2 and 3, by way of example, the biomolecular characteristic identification method in accordance with the preferred embodiment of the present invention includes the step S2: automatically, semi-automatically or manually seeking at least one nucleotide mark in the baited Taiwan Tilapias to obtain the at least one feature sequence marker 1a (e.g., cold hardiness marker, disease resistance marker, salinity tolerance marker) therefrom.

[0060] Still referring to FIGS. 1, 2 and 3, by way of example, the biomolecular characteristic identification method in accordance with the preferred embodiment of the present invention includes the step S3: automatically, semi-automatically or manually producing at least one design of primer pair (i.e., artificial sequence) 3a according to the feature sequence marker 1a for identification with it.

[0061] Still referring to FIGS. 1, 2 and 3, by way of example, the biomolecular characteristic identification method in accordance with the preferred embodiment of the present invention includes the step S4: automatically, semi-automatically or manually identifying an unknown DNA sample of the baited Taiwan Tilapias (i.e., unknown Taiwan Tilapias organism 20) with the at least one primer pair 3a in a biomolecular tracing procedure to obtain an identification result 40.

[0062] With continued reference to FIGS. 1, 2 and 3, by way of example, a DNA sample of unknown Taiwan Tilapias organism 20 is extracted in the extraction device 1 and can be tested by the DNA testing device 3 with various suitable manners, such as: electrophoresis, capillary electrophoresis, PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) or other suitable manners.

[0063] Still referring to FIGS. 1, 2 and 3, by way of example, the nucleotide mark can be selected from various cold hardiness markers 10a (e.g., Seq. 1, Seq. 2, Seq. 5, Seq. 6, Seq. 9, Seq. 10, Seq. 13, Seq. 14, Seq. 17, Seq. 18), various salinity tolerance markers 10b (e.g., Seq. 21, Seq. 24, Seq. 27, Seq. 30, Seq. 33), various disease resistance markers 10c (e.g., Seq. 36, Seq. 39, Seq. 42, Seq. 45, Seq. 48).

[0064] By way of example, the cold hardiness marker can be selected from a (ATT).sub.n-sequence-contained marker as follows:

TABLE-US-00001 Seq. 1:GACTACATCCCAGCAACAGTGACACATTCCACCGCGAGCCCAC CCTCCACCTTCCCAACACTACCATTATTATTATTATTATTATTATTATTA TTATTATTATTATTATTTGTATTTTTTTACTTGGAGCAACCCGCCACGCT CCCCTTGCACATGGACTGGAAAGATGATAGCCT

TABLE-US-00002 Seq. 2:GACTACATCCCAGCAACAGTGACACATTCCACCGCGAGCCCAC CCTCCACCTTCCCAACACTACTATTATTATTATTATTATTATTATTATTA TTATTATTATTATTATTATTATTATTATTTTTATTTTTTTACTTGGAGCA ACCCGCCACGCTCCCCTTGCACATGGACTGGAAAGATGATAGCCT

[0065] By way of example, the (ATT).sub.n-sequence-contained marker can be identified by forward primer Seq. 3 and reverse primer Seq. 4 of primer pair 3a as follow:

TABLE-US-00003 Seq. 3: GACTACATCCCAGCAACAGTGA

TABLE-US-00004 Seq. 4: AGGCTATCATCTTTCCAGTCCA

[0066] By way of example, the cold hardiness marker can be selected from a (G).sub.n-sequence-contained marker as follows:

TABLE-US-00005 Seq. 5:ATGAGGTGAACAAAAGCCAATGGTTTCCAGACTCCACCCAAAC ACAACCTTTTAGTGTTTTTTAAAATAAAGCCTAAATGGGGGGGGGGGGGG TCGTGTTCAGCCCCCACTTGCTTACGCTTTACTAAGCGGCGAGTTAAGTC TCTGTCAAGGA

TABLE-US-00006 Seq. 6:ATGAGGTGAACAAAAGCCAATGGTTTCCAGACTCCACCCAAAC ACAACCTTTTAGTGTTTTTTAAAATAAAGCCTAAATGGGGGGGGGGGTCG TGTTCAGCCCCCACTTGCTTACGCTTTACTAAGCGGCGAGTTAAGTCTCT GTCAAGGA

[0067] By way of example, the (G).sub.n-sequence-contained marker can be identified by forward primer Seq. 7 and reverse primer Seq. 8 of primer pair 3a as follow:

TABLE-US-00007 Seq. 7: ATGAGGTGAACAAAAGCCAATGG

TABLE-US-00008 Seq. 8: TCCTTGACAGAGACTTAACTCGC

[0068] By way of example, the cold hardiness marker can be selected from a (GAG).sub.n-sequence-contained marker as follows:

TABLE-US-00009 Seq. 9:TATGGGCTGGAGAGGAGACTAAAGGAGGTGTGAGAGGAGGCGC CTCCTTATAGGACAGTGCTCTCGCTCTCCAGGTCACAGTCTTTAGGAAGA GGCGGCGAGGAGGAGGAGGAGGAGGAGAAGGCGGTGGCAGTCGGCAGCTG CTGGTCTACTGCCGTGAAGTC

TABLE-US-00010 Seq. 10:TATGGGCTGGAGAGGAGACTAAAGGAGGTGTGAGAGGAGGCG CCTCCTTATAGGACAGTGCTCTCGCTCTCCAGGTCACAGTCTTTAGGAAG AGGCGGCGAGGAGGAGAAGGCGGTGGCAGTCGGCAGCTGCTGGTCTACTG CCGTGAAGTC

[0069] By way of example, the (GAG).sub.n-sequence-contained marker can be identified by forward primer Seq. 11 and reverse primer Seq. 12 of primer pair 3a as follow:

TABLE-US-00011     Seq. 11: TATGGGCTGGAGAGGAGACTAA

TABLE-US-00012 Seq. 12: GACTTCACGGCAGTAGACCAG

[0070] By way of example, the cold hardiness marker can be selected from a (GT).sub.n-sequence-contained marker as follows:

TABLE-US-00013 Seq. 13:GAGCGTTGTTGTGTTTCTGCTATTAAGTGTGTGTGTGTGTGT AGCACCAGCATGATGTCGGACTTTGACATCATGCTCGCCCGGAGGAAAGC CATGAACAGCAAGAAGAG

TABLE-US-00014 Seq. 14:GAGCGTTGTTGTGTTTCTGCTATTAAAAAAGTGTGTGTGTGT GTGTGTGTGTGTGTGTGTGTGTGTGTGTAGCACTAGCGTGATGTCGGACT TCGACATCATGCTCGCCCGGAGGAAAGCCATGAACAGCAAGAAGAG

[0071] By way of example, the (GT).sub.n-sequence-contained marker can be identified by forward primer Seq. 15 and reverse primer Seq. 16 of primer pair 3a as follow:

TABLE-US-00015 Seq. 15: GAGCGTTGTTGTGTTTCTGCTA

TABLE-US-00016 Seq. 16: CTCTTCTTGCTGTTCATGGCTT

[0072] By way of example, the cold hardiness marker can be selected from a (CA).sub.n-sequence-contained marker as follows:

TABLE-US-00017 Seq. 17:ACAGCAGGAGGAGAGGAGAGAGGGGAGGAGCAGACTGAACTT CATAGACACCGGTCCCCTCGGCTCACCCCTCTCATTCACACACACACACA CACACACACACACAGTCGCACCTAATGTGAATACTTTGGGTTTGTGGCAG CAGTGTGGATGTTTCTTTGTGTCGCTGAACT

TABLE-US-00018     Seq. 18:ACAGCAGGAGGAGAGGAGAGAGGGGAGGAGCAGACTGA ACTTCATAGACACCGGTCCCCTCGGCTCACCCCTCTCATTCACACACACA CACACACACACACACACACACACACACACACACAGTCGCACCTAATGTGA ATACTTTGGGTTTGTGGCAGCAGTGTGGATGTTTCTTTGTGTCGCTGAAC T

[0073] By way of example, the (CA).sub.n-sequence-contained marker can be identified by forward primer Seq. 19 and reverse primer Seq. 20 of primer pair 3a as follow:

TABLE-US-00019 Seq. 19: ACAGCAGGAGGAGAGGAGAGA

TABLE-US-00020 Seq. 20: AGTTCAGCGACACAAAGAAACA

[0074] By way of example, the salinity tolerance marker can be selected from a (CA).sub.11-sequence-contained marker as follows:

TABLE-US-00021 Seq. 21:ACTCCCATACTGTATTTGCGTGTCTGTTTGTGTGTGCAGAGC AGGTTCTCTGGTCATACACACGCACACACACACACACACACACACGCCCT GTCGATTTCTTATTTCTGCTGATAAAGAAGAGGAGGCACTTGTGAAATGA AC

[0075] By way of example, the (CA).sub.11-sequence-contained marker can be identified by forward primer Seq. 22 and reverse primer Seq. 23 of primer pair 3a as follow:

TABLE-US-00022     Seq. 22: ACTCCCATACTGTATTTGCGTGT

TABLE-US-00023 Seq. 23: GTTCATTTCACAAGTGCCTCCTC

[0076] By way of example, the salinity tolerance marker can be selected from a (AT).sub.8-sequence-contained marker as follows:

TABLE-US-00024     Seq. 24:AGCAGATTAACATTGAAATAGGCTGTAAAAAAAAAATA GAGAAAAGCACAGAAAATAATAGACAAAGGTCTGTCTGAATATATATATA TATATATACATACACTATCTTAAAAATTAAAAAAAAAAACATTAAACATA AGGTAGTGTAAAAACAAGTTGTATAGACAATGAAATACAAAATCATCTCA TAAAAGAATTAATGTAATTCTGTGTTTCTGAATTTGCTTTTCTACTCACA CAG

[0077] By way of example, the (AT).sub.8-sequence-contained marker can be identified by forward primer Seq. 25 and reverse primer Seq. 26 of primer pair 3a as follow:

TABLE-US-00025     Seq. 25: AGCAGATTAACATTGAAATAGGCT

TABLE-US-00026 Seq. 26: CTGTGTGAGTAGAAAAGCA

[0078] By way of example, the salinity tolerance marker can be selected from a (TAT).sub.8-sequence-contained marker as follows:

TABLE-US-00027     Seq. 27:TAAAGCAACTTTTGTGGGCAAGTTTGATCTGCTGGGTA TATTATTATTATTATTATTATTATTGTTATTGTTGGTTATTATACACATG GTGGCTCTGCTTGTAAAGTGAAA

[0079] By way of example, the (TAT).sub.8-sequence-contained marker can be identified by forward primer Seq. 28 and reverse primer Seq. 29 of primer pair 3a as follow:

TABLE-US-00028     Seq. 28: TAAAGCAACTTTTGTGGGCAAGT

TABLE-US-00029 Seq. 29: TTTCACTTTACAAGCAGAGCCAC

[0080] By way of example, the salinity tolerance marker can be selected from a (TTTG).sub.5-sequence-contained marker as follows:

TABLE-US-00030     Seq. 30:AGGGTTTAAAGAGAGTTTGGCCTAGTCAACATATTTTT GTTTGTTTGTTTGTTTGTATGTATGTGTGTTTGTTTTGAATTGTGTTGAG CTGAACTAGATTTTGCATCTCTATAAAGAATTCAGGAATCCACTGTCTAA GGGCTA

[0081] By way of example, the (TTTG).sub.5-sequence-contained marker can be identified by forward primer Seq. 31 and reverse primer Seq. 32 of primer pair 3a as follow:

TABLE-US-00031     Seq. 31: AGGGTTTAAAGAGAGTTTGGCCT

TABLE-US-00032 Seq. 32: TAGCCCTTAGACAGTGGATTCCT

[0082] By way of example, the salinity tolerance marker can be selected from a (AT).sub.12-sequence-contained marker as follows:

TABLE-US-00033     Seq. 33:CAGTGTGAGTTTAACTTCGGGTCCAAGCCTTTCCGTCA CCCATATATATATATATATATATATATGTATATATAATTTTTTTGTTATT TTTTTGTGTATTTTTTATTTTTTTCCTCCCCAGTCTGGGATACCCACTAA AATG

[0083] By way of example, the (AT).sub.12-sequence-contained marker can be identified by forward primer Seq. 34 and reverse primer Seq. 35 of primer pair 3a as follow:

TABLE-US-00034     Seq. 34: CAGTGTGAGTTTAACTTCG

TABLE-US-00035 Seq. 35: CATTTTAGTGGGTATCC

[0084] By way of example, the disease resistance marker can be selected from a (CAGG).sub.7-sequence-contained marker as follows:

TABLE-US-00036     Seq. 36:GCACAGACACAGTAACACATGCACTGAGTATTCATTTG ACTGAGAGTATGTGCTAAATTTGATTTGGTTTTGTTTTCAGGCTTGTCAG CTGCCATTTAAACATGCATAGACAGCCAGGCAGGCAGGCAGGCAGGCAGG CAGGCGTCTGTGTGTCCCTGTTTCTGTTTGAAGTGGATGATCTCTGATCC CACGTACCTCTCTGATTGGGACATGCGTAGTCTCTCTCCGTCTGTCTGTT TTCCTTCTTCACCCCACGAAAGCTTTCTGAATCGAGTCAGGCAAAGCTGA AATAATACTGTTTGGAAAGGAAAATTAAATGACATAAAGAACTGTTTCTT TAATAAATGAAATAGGAAGCATGTACCAGGGAGT

[0085] By way of example, the (CAGG).sub.7-sequence-contained marker can be identified by forward primer Seq. 37 and reverse primer Seq. 38 of primer pair 3a as follow:

TABLE-US-00037     Seq. 37: GCACAGACACAGTAACACATGCAC

TABLE-US-00038     Seq. 38: ACTCCCTGGTACATGCTTCCTA

[0086] By way of example, the disease resistance marker can be selected from a (CTAC).sub.7-sequence-contained marker as follows:

TABLE-US-00039     Seq. 39:CACCACTGTCAACTGGCTAATGGACCTAAGGGCAGACT ATCTATCTACCTATCTACCTACCTACCTACCTACCTACCTACGTACCTAC CTACAAACAATACACATGCACCCCAACTAATATTTGTTTAAGTGTCCCTT GGGAATCTGCACCCCAACCACATGCTTTGGTAGCCTGTCTAAACTGTTTT AACTTGCAACATATATCAGGGTTTGATACCTGCCAACAGTAGTGAAATCT CATATTGAATTAAATGACCAAAATCTTCTTCAAAGTAGCTTTATACTCGA ATCGATTTTCTAGAATTAAATTCAAACATATTTTACAGTTGTGATAGTGC CTGCGGTATCAAGAAGGTAAC

[0087] By way of example, the (CTAC).sub.7-sequence-contained marker can be identified by forward primer Seq. 40 and reverse primer Seq. 41 of primer pair 3a as follow:

TABLE-US-00040     Seq. 40: CACCACTGTCAACTGGCTAATG

TABLE-US-00041 Seq. 41: GTTACCTTCTTGATACCGCAGG

[0088] By way of example, the disease resistance marker can be selected from a (TTGA).sub.20-sequence-contained marker as follows:

TABLE-US-00042     Seq. 42:GGACCCTGAATCTTCCCTTAGTTATGCTGCAAAAAGTG TAGAATGCTGGGAGATTCCCATGATGCATTGTGTATTTCTTCTTCAGTCG TCTTTTTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGAT TGATTGATTGATTGATTGATTGATTGATTGATTGACTGGAGGAGAGCAGT TTGAGAACAGGAAAAAACAGCCATCTGGTCCTGCAGCCATACTCCGTCGC TGGAATCAGACCCACCTGGCTGCATCGAGTGGAAGTAGTCATTTATATTG TATATTGTATATTTCTGTCTGAGTACATGTGCATGTGTGCGTAAGTGTCT GTATCTTGTGTCCAACTCAAGAGAGAGAGCC

[0089] By way of example, the (TTGA).sub.20-sequence-contained marker can be identified by forward primer Seq. 43 and reverse primer Seq. 44 of primer pair 3a as follow:

TABLE-US-00043     Seq. 43: GGACCCTGAATCTTCCCTTAGT

TABLE-US-00044 Seq. 44: GGCTCTCTCTCTTGAGTTGGACA

[0090] By way of example, the disease resistance marker can be selected from a (TTGT).sub.10-sequence-contained marker as follows:

TABLE-US-00045     Seq. 45:ACTAGCAGATGATAAATGCGCCAGAAAATAAACGCTTA AAGGGAATATGCACCAGTCCAGCTGTTTGTTTGTTTGTTTGTTTGTTTGT TTGTTTGTTTGTTTTACTGCTCAAATTTTGTATTTGCACTGTTTCGGCTC AGTGTGACAGTTTTTTTGGTGTCAGCTCAGATTGCGAGGACATCTCTTGT GCGCAGACCTCTTCAGGGCACCCCACAGATTTTCTGTAGGATTTAGGTCT GGGCAT

[0091] By way of example, the (TTGT).sub.10-sequence-contained marker can be identified by forward primer Seq. 46 and reverse primer Seq. 47 of primer pair 3a as follow:

TABLE-US-00046     Seq. 46: ACTAGCAGATGATAAATGCGC

TABLE-US-00047 Seq. 47: ATGCCCAGACCTAAATCCTACA

[0092] By way of example, the disease resistance marker can be selected from a (GAAAA).sub.6-sequence-contained marker as follows:

TABLE-US-00048     Seq. 48:TGTATGTAGCCGAAGTAGCCAATCTCAACAGTACTTGC TTGTTTTAATTTATTTACTGGTTTGGTGCACTTTGTATTTAAGAAAAAAA ATTGATAACTGTAAACCACCATGGCAGACCCGATGGCATTTTCATGCTTC GCAACATCATTAGCGAATGATGTCTGTTCTGAAAAGAAAAGAAAAGAAAA GAAAAGAAAAGACCTTGACGTCCGTGGAAGTCAACAGCGGTGGATGACTG CAGAATCATTCGTGCGATCATCCACCACTGTTGAGAAACCCCTTCACAAC AGCCAACAAAATGAACAACACTCTGCAGGAGGAAGGCGTATCCAAGTATA CCATAAAGAGAAGACTGCAGGTAAGTAAATACAAAGGGTTCACTGCAAGG

[0093] By way of example, the (GAAAA).sub.6-sequence-contained marker can be identified by forward primer Seq. 49 and reverse primer Seq. 50 of primer pair 3a as follow:

TABLE-US-00049     Seq. 49: TGTATGTAGCCGAAGTAGCCAA

TABLE-US-00050 Seq. 50: CCTTGCAGTGAACCCTTTGTAT

[0094] Referring back to FIGS. 1, 2 and 3, by way of example, the biomolecular characteristic identification method in accordance with the preferred embodiment of the present invention includes: automatically, semi-automatically or manually transmitting the identification result 40 to a device (e.g., computer display device, mobile communication device or other devices) for displaying a correct source of original aquatic strain or product or an incorrect source of original aquatic strain or product.

[0095] FIG. 4 is a schematic block diagram of an exogenous biomolecular tracing system applied in the biomolecular characteristic identification method and system thereof for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention. Turning now to FIG. 4, an exogenous biomolecular tracing system in accordance with the preferred embodiment of the present invention includes a plurality of feature sequence markers 11, a basic material 12, a plurality of Taiwan Tilapia organisms (exogenous aquatic creatures or products) 2 and at least one primer pair 30.

[0096] FIG. 5 is a flow chart of an exogenous biomolecular tracing system applied in the biomolecular characteristic identification method and system thereof for Taiwan Tilapia strains in accordance with a preferred embodiment of the present invention. Turning now to FIGS. 4 and 5, the exogenous biomolecular tracing method in accordance with the preferred embodiment of the present invention includes the step S1a: automatically, semi-automatically or manually extracting the feature sequence marker 11 from a marker source organism 10 with the extraction device 1.

[0097] With continued reference to FIGS. 4 and 5, by way of example, the feature sequence marker (nucleotide marker) 11 can be selected from a DNA extract, a DNA extract liquid, a DNA extract powder (e.g., freeze-dried powder), a DNA extract-related material or combinations thereof.

[0098] Still referring to FIGS. 4 and 5, by way of example, the exogenous biomolecular tracing method in accordance with the preferred embodiment of the present invention includes the step S2a: automatically, semi-automatically or manually combining the basic material 12 with the feature sequence marker 11 in an auto-device (i.e., feeder device, stirrer device or combination thereof) to form a nucleotide marker-contained material.

[0099] Still referring to FIGS. 4 and 5, by way of example, the exogenous biomolecular tracing method in accordance with the preferred embodiment of the present invention includes the step S3a: exogenously combining the nucleotide marker-contained material with the Taiwan Tilapia organisms 2 in an auto-device (i.e., feed-supplying device, dipping device, painting device, sprayer device or combinations thereof) to form an exogenously-marked Taiwan Tilapia organism.

[0100] Still referring to FIGS. 4 and 5, by way of example, exogenously combining the nucleotide marker-contained material with the Taiwan Tilapia organisms 2 includes a feeding process, a dipping process, a painting process, a spraying process or combinations thereof. In another embodiment, by way of example, the nucleotide marker-contained material can be selectively formed as an oral vaccine or a vaccine product.

[0101] Still referring to FIGS. 4 and 5, by way of example, the exogenous biomolecular tracing method in accordance with the preferred embodiment of the present invention includes the step S4a: automatically, semi-automatically or manually identifying a DNA sample received from the exogenously-marked Taiwan Tilapia organism (e.g., aquatic creature or aquatic product) with at least one primer pair 30 in an exogenous biomolecular tracing procedure with the DNA testing device 3 to obtain an exogenously-marked identification result 4.

[0102] Although the invention has been described in detail with reference to its presently preferred embodiment, it will be understood by one of ordinary skills in the art that various modifications can be made without departing from the spirit and the scope of the invention, as set forth in the appended claims.