METHOD FOR REDUCING THE NEGATIVE EFFECT OF HEAT ACCLIMATIZATION FOR HATCHING OF POULTRY HATCHING EGG

Abstract

A method for reducing the negative effect of heat acclimatization for the hatching of a poultry hatching egg is provided. A special light source is used as an ambient light to hatch the poultry hatching egg. The special light source is light-emitting diode (LED) monochromatic blue light with a light intensity of 20 lux to 50 lux and a wavelength of about 450 nm, such that the light can reduce the negative effect of heat acclimatization at a hatching stage of the poultry hatching egg. The method can reduce the levels of oxidation products such as malondialdehyde (MDA) and corticosterone in a chicken embryo during heat acclimatization at the hatching stage of the poultry hatching egg and increase the expression levels of antioxidase and heat shock protein (HSP) genes, and the special light source reduces the metabolic heat production of the chicken embryo.

Claims

1. A method for reducing a negative effect of heat acclimatization for hatching of a poultry hatching egg, comprising: conducting a light treatment during the hatching of the poultry hatching egg; wherein during the hatching of the poultry hatching egg, while the poultry hatching egg is subjected to heat acclimatization, a specific light treatment is conducted, wherein a blue light is used in the specific light treatment, the blue light has a wavelength of 400 nm to 480 nm, and the light treatment is conducted for 1 h to 24 h every day with a light intensity of 10 lux to 800 lux.

2. The method for reducing the negative effect of heat acclimatization for the hatching of the poultry hatching egg according to claim 1, wherein during the hatching of the poultry hatching egg, while the poultry hatching egg is subjected to heat acclimatization, the poultry hatching egg is irradiated with the blue light.

3. The method for reducing the negative effect of heat acclimatization for the hatching of the poultry hatching egg according to claim 1, wherein the heat acclimatization comprises, but is not limited to, intermittent exposure of the poultry hatching egg to a high-temperature environment at a hatching stage to allow a hatched chicken to have high heat resistance.

4. The method for reducing the negative effect of heat acclimatization for the hatching of the poultry hatching egg according to claim 3, wherein the high-temperature environment has a temperature of 40.0±1° C.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0023] FIG. 1 is a three-dimensional (3D) view illustrating a structure of the light environment-controlled hatching system of the present disclosure, where 1 represents an incubator, 2 represents an LED tube, 3 represents an egg-flipping iron frame, 4 represents an egg tray, and 5 represents a poultry hatching egg.

[0024] FIG. 2 is a side view illustrating a structure of the light environment-controlled hatching system of the present disclosure, where numerals in FIG. 2 are the same as in FIG. 1.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0025] The embodiments of the present disclosure are described below, and the actual use of the present disclosure is not limited to the embodiments.

[0026] As shown in FIG. 1, an incubator with a temperature and humidity controllable is used as a hatching environment for a poultry hatching egg. In this embodiment, the incubator 1 has a size of 90 cm×62 cm×74 cm, and an egg-flipping iron frame 3 with a length of 78 cm and a width of 55 cm is placed in the incubator 1. An LED tube 2 is fixed at each of the two inner sides of the egg-flipping iron frame 3 to provide a light environment for a hatching experiment. A plurality of egg trays 4 is placed on the egg-flipping iron frame to hold and stabilize poultry hatching eggs 5. Four incubators each with such a configuration are provided to set up three experimental groups and a parallel control group.

[0027] The three experimental groups are as follows: LED blue light irradiation+heat acclimatization; LED blue light irradiation+no heat acclimatization; and LED white light irradiation+heat acclimatization. The parallel control group is as follows: LED white light irradiation+no heat acclimatization.

[0028] In a specific implementation, blue light used for the LED blue light irradiation group has a wavelength of about 450 nm and a light intensity of 20 lux to 50 lux; white light used for the LED white light irradiation group has a normal full-wavelength spectrum and a light intensity of 20 lux to 50 lux; in the heat acclimatization group, a temperature is 40.0±1° C. when heat acclimatization is conducted and a temperature is 37.6±1° C. when heat acclimatization is not conducted; and in the no heat acclimatization group, a temperature is always kept at 37.6±1° C. In all groups, the relative humidity is kept at 60±1%.

[0029] A specific experimental process is divided into three parts: pre-hatching, heat acclimatization, and sampling and detection.

[0030] 800 hatching eggs of yellow-footed hemp breeders are selected for hatching, and 200 hatching eggs are randomly assigned to each incubator. The eggs each are flipped for 2 h daily from day 1 to day 18. The embryo survival is checked on day 18, and hatching eggs with dead embryos are eliminated. The temperature of each incubator is kept at 37.6±1° C. before heat acclimatization.

[0031] From day 14 to day 18 of hatching, hatching eggs of the heat acclimatization group each is subjected to heat acclimatization, that is, the hatching eggs each are exposed to an ambient temperature of 40.0±1° C. every day consecutively for 4 h, and after the heat acclimatization is completed, the temperature is reduced to 37.6±1° C.

[0032] The sampling and detection is conducted at the end of the entire heat acclimatization phase, namely, on day 18 of hatching. 24 eggs are randomly selected from each group for sampling. The collected samples are embryonic liver tissues, and are tested as follows: ½ of the liver tissues are tested by a biological diagnosis kit to determine the levels of oxidation products such as MDA and corticosterone and determine the activities of SOD and catalase; and ½ of the liver tissues are used to determine the gene transcription levels of HSP, heat shock factor (HSF), and antioxidases.

[0033] Test results are shown in Table 1.

TABLE-US-00001 TABLE 1 Oxidation product levels and heat resistance gene expression levels of chicken HSP70 HSP90 HSF1 HSF3 SOD CAT MDA (average (average (average (average (average (average Corticoste- (nmol/ fold fold fold fold fold fold Group rone (ng/g) mg) change) change) change) change) change) change) Blue light + heat acclimatization 3.366 60.475 2.15 1.94 1.25 1.09 0.83 1.24 Blue light + no heat 2.670 52.789 1.15 1.08 0.99 1.70 1.12 1.13 acclimatization White light + heat 3.764 69.815 2.98 2.30 1.21 2.06 0.71 0.90 acclimatization White light + no heat 2.842 53.343 1.04 1.04 1.03 1.03 1.02 1.02 acclimatization

[0034] It can be seen from the results that the levels of oxidation products in the chicken embryos of the LED blue light irradiation+heat acclimatization group are significantly lower than that in the chicken embryos of the heat acclimatization group without LED blue light irradiation (P<0.01). It can be seen from the test results of gene transcription levels of embryonic liver cells that the LED blue light irradiation significantly increases the transcription levels of HSP 70 (P<0.01), HSP 90 (P<0.05), SOD (P<0.01), and catalase (P<0.01) genes.

[0035] It can be seen from the above results that the LED blue light irradiation+heat acclimatization at a hatching stage can significantly increase the expression levels of embryonic HSP and antioxidases and reduce the ROS content in vivo, thereby reducing a negative effect caused by heat acclimatization; and the LED blue light irradiation can alleviate oxidative stress of a chicken embryo liver caused by heat stress and improve the welfare of animal farming.