COSMETIC COMPOSITION FOR SKIN IMPROVEMENT COMPRISING, AS ACTIVE INGREDIENTS, POLYSACCHARIDES, YEAST EXTRACT, AND STRAIN FERMENTATION PRODUCT WITH CHARACTERISTICS OF PROBIOTICS

Abstract

The present invention relates to a cosmetic composition, which is safe for the human body and has a skin condition improving effect by containing, as active ingredients, polysaccharides, a yeast extract, and a strain fermentation product with characteristics of probiotics. The cosmetic composition can be provided for improving microbial flora inhabiting the skin, soothing the skin, relieving skin wrinkles, improving skin elasticity, soothing the scalp, relieving the oiliness of the scalp, preventing hair loss, or improving hair growth.

Claims

1-17. (canceled)

18. A cosmetic composition for skin improvement, comprising a polysaccharide, a yeast extract, and a strain fermentation product with probiotic characteristics as active ingredients.

19. The cosmetic composition of claim 18, wherein the polysaccharide is one or more selected from the group consisting of inulin, beta-glucan, and maltodextrin.

20. The cosmetic composition of claim 18, wherein the yeast extract is one or more selected from the group consisting of a beer-derived yeast extract and a truffle-derived yeast extract.

21. The cosmetic composition of claim 18, wherein a strain with the probiotic characteristics is one or more selected from the group consisting of Lactobacillus sp. strain and Bifidobacterium sp. strain.

22. The cosmetic composition of claim 21, wherein the fermentation product is one or more selected from the group consisting of a ferment lysate and a ferment filtrate.

23. The cosmetic composition of claim 18, comprising inulin, beta-glucan, maltodextrin, a beer-derived yeast extract, a truffle-derived yeast extract, a Lactobacillus ferment lysate, and a Bifidobacterium ferment lysate as active ingredients.

24. The cosmetic composition of claim 23, comprising inulin, beta-glucan, maltodextrin, the beer-derived yeast extract, the truffle-derived yeast extract, the Lactobacillus ferment lysate, and the Bifidobacterium ferment lysate in the same weight ratio, respectively.

25. The cosmetic composition of claim 23, comprising one or more active ingredients selected from the group consisting of inulin, beta-glucan, maltodextrin, the beer-derived yeast extract, the truffle-derived yeast extract, the Lactobacillus ferment lysate, and the Bifidobacterium ferment lysate in an amount of 0.00001 to 10 wt% with respect to 100 wt% of the cosmetic composition.

26. The cosmetic composition of claim 18, wherein the cosmetic composition is for improving microbial flora inhabiting the skin.

27. The cosmetic composition of claim 26, wherein the cosmetic composition is for promoting growth of beneficial bacteria inhabiting the skin or suppressing growth of harmful bacteria.

28. The cosmetic composition of claim 27, wherein the beneficial bacteria is Staphylococcus epidermidis (S. epidermidis), and the harmful bacteria is Staphylococcus aureus (S. aureus).

29. The cosmetic composition of claim 18, wherein the cosmetic composition is for any one of skin soothing, skin wrinkle amelioration, and skin elasticity improvement.

30. The cosmetic composition of claim 18, wherein the skin is scalp.

31. The cosmetic composition of claim 30, wherein the cosmetic composition is for any one of scalp soothing, scalp oil improvement, hair loss prevention, and hair growth promotion.

32. The cosmetic composition of claim 30, wherein the cosmetic composition is for improving microbial flora inhabiting the scalp.

33. A method for improving skin, comprising applying, to the skin, a cosmetic composition for skin improvement including a polysaccharide, a yeast extract, and a strain fermentation product with probiotic characteristics as active ingredients.

34. The method of claim 33, wherein the polysaccharide is one or more selected from the group consisting of inulin, beta-glucan, and maltodextrin.

35. The method of claim 33, wherein the yeast extract is one or more selected from the group consisting of a beer-derived yeast extract and a truffle-derived yeast extract.

36. The method of claim 33, wherein a strain with the probiotic characteristics is one or more selected from the group consisting of Lactobacillus sp. strain and Bifidobacterium sp. strain.

37. The method of claim 33, wherein the method is any one of a method for improving microbial flora inhabiting the skin, a skin soothing method, a skin wrinkle amelioration method, a skin elasticity improvement method, a method for improving microbial flora inhabiting scalp, a scalp soothing method, a scalp oil improvement method, a hair loss prevention method, and a hair growth promotion method.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0062] FIGS. 1 shows photographs of scalp (A) before application and (B) after a single application of a hair shampoo in accordance with an embodiment of the present disclosure.

[0063] FIGS. 2 shows photographs of scalp (A) before application and (B) after a single application of a hair shampoo in accordance with an embodiment of the present disclosure.

[0064] FIGS. 3 shows photographs of scalp (A) before application and (B) after a single application of a hair shampoo in accordance with an embodiment of the present disclosure.

BEST MODE FOR CARRYING OUT THE INVENTION

[0065] The objects, specific advantages, and novel features of an embodiment of the present disclosure will become more apparent from the following detailed description and preferred examples. However, these examples are for illustrative purposes only, and the scope of the present disclosure is not limited to these examples. In addition, in describing the present disclosure, if it is determined that a detailed description of a related known technology may unnecessarily obscure the gist of the present disclosure, the detailed description thereof will be omitted.

[0066] Manufacturing example. Complex including polysaccharides, yeast extract, and strain fermentation product with probiotic characteristics

[0067] Used in the following examples were commercially available products including inulin (Beneo), beta-glucan (Quegen Biotech Co.,Ltd.), maltodextrin (Samyang), and a beer-derived yeast extract (Activon). Used as a truffle-derived yeast extract was an extract extracted from a product obtained by isolating yeast from truffle and performing culture at 37° C. for 48 hours, followed by sterilization. Used as a Lactobacillus ferment lysate and a Bifidobacterium ferment lysate were an extract extracted from a product obtained by culturing Lactobacillus and Bifidobacterium (Chr. Hansen) at 37° C. for 48 hours and then sterilizing the same.

[0068] (Preparation Example 1) A complex was prepared by mixing each 10 g of inulin, the beer-derived yeast extract, and the Lactobacillus ferment lysate. (Preparation Example 2) A complex was prepared by mixing each 10 g of beta-glucan, the beer-derived yeast extract, and the Lactobacillus ferment lysate. (Preparation Example 3) A complex was prepared by mixing each 10 g of maltodextrin, the beer-derived yeast extract, and the Lactobacillus ferment lysate. (Preparation Example 4) A complex was prepared by mixing each 10 g of inulin, the truffle-derived yeast extract, and the Bifidobacterium ferment lysate. (Preparation Example 5) A complex was prepared by mixing each 10 g of beta-glucan, the truffle-derived yeast extract, and the Bifidobacterium ferment lysate. (Preparation Example 6) A complex was prepared by mixing each 10 g of maltodextrin, the truffle-derived yeast extract, and the Bifidobacterium ferment lysate. (Preparation Example 7) A complex was prepared by mixing each 10 g of inulin, beta-glucan, maltodextrin, the beer-derived yeast extract, the truffle-derived yeast extract, the Lactobacillus ferment lysate, and the Bifidobacterium ferment lysate.

[0069] Example. Cosmetic composition including polysaccharide, yeast extract, and strain fermentation product with probiotic characteristics as active ingredients

Example 1-1. Hair Shampoo Composition

[0070] A hair shampoo composition having a composition as shown in Table 1 below was prepared.

TABLE-US-00001 Ingredient name Content (wt%) Ingredient name Content (wt%) Disodium laureth sulfosuccinate 5.000 Inulin 0.001 Lauryl hydroxysultaine 5.000 Beta-glucan 0.001 Disodium lauryl sulfosuccinate 1.000 Maltodextrin 0.001 Glycerin 1.000 Beer-derived yeast extract 0.001 Caprylyl glycol 1.000 Truffle-derived yeast extract 0.001 Tetrasodium EDTA 0.500 Lactobacillus ferment lysate 0.001 Spices Appropriate amount Bifidobacterium ferment lysate 0.001 pH adjuster Appropriate amount Purified water Remaining amount Preservative Appropriate amount Total 100

Example 1-2. Hair Shampoo Composition

[0071] A hair shampoo composition having a composition as shown in Table 2 below was prepared.

TABLE-US-00002 Ingredient name Content (wt%) Ingredient name Content (wt%) Disodium laureth sulfosuccinate 10.000 Inulin 0.001 Cocamidopropyl 5.000 Beta-glucan 0.001 betaine Glycerin 1.000 Maltodextrin 0.001 Caprylyl glycol 1.000 Beer-derived yeast extract 0.001 Polyquaternium-7 0.500 Truffle-derived yeast extract 0.001 Spices Appropriate amount Lactobacillus ferment lysate 0.001 pH adjuster Appropriate amount Bifidobacterium ferment lysate 0.001 Preservative Appropriate amount Purified water Remaining amount Total 100

Example 2. Hair Treatment Composition

[0072] A hair treatment composition having a composition as shown in Table 3 below was prepared.

TABLE-US-00003 Ingredient name Content (wt%) Ingredient name Content (wt%) Ethanol 1.000 Inulin 0.001 Cetearyl alcohol 20.000 Beta-glucan 0.001 Stearamidopropyl dimethylamine 5.000 Maltodextrin 0.001 Shea butter 5.000 Beer-derived yeast extract 0.001 Macadamia seed oil 5.000 Truffle-derived yeast extract 0.001 PEG-100 stearate 5.000 Lactobacillus ferment lysate 0.001 Polyquaternium-7 0.500 Bifidobacterium ferment lysate 0.001 pH adjuster Appropriate amount Spices Appropriate amount Preservative Appropriate amount Purified water Remaining amount Total 100

Example 3. Hair Tonic

[0073] A hair tonic composition having a composition as shown in Table 4 below was prepared.

TABLE-US-00004 Ingredient name Content (wt%) Ingredient name Content (wt%) Ethanol 30.000 Octyldodeces-16 0.200 Lightyellow 0.005 Inulin 0.001 Sophora root extract Niacinamide 0.100 Beta-glucan 0.001 Menthol 0.100 Maltodextrin 0.001 Guinea pepper extract 0.050 Beer-derived yeast extract 0.001 Benzyl nicotinate 0.005 Truffle-derived yeast extract 0.001 Asarum canadense root extract 0.300 Lactobacillus ferment lysate 0.001 Spices Appropriate amount Bifidobacterium ferment lysate 0.001 pH adjuster Appropriate amount Purified water Remaining amount Total 100

Example 4. Body Wash

[0074] A body wash composition having a composition as shown in Table 5 below was prepared.

TABLE-US-00005 Ingredient name Content (wt%) Ingredient name Content (wt%) Trisodium EDTA 0.050 Inulin 0.001 Disodium cocoyl glutamate 4.000 Beta-glucan 0.001 Sodium cocoyl alaninate 3.000 Maltodextrin 0.001 Lauramidopropyl betaine 3.000 Beer-derived yeast extract 0.001 PEG-40 hydrogenated castor oil 1.000 Truffle-derived yeast extract 0.001 Spices Appropriate amount Lactobacillus ferment lysate 0.001 pH adjuster Appropriate amount Bifidobacterium ferment lysate 0.001 Preservative Appropriate amount Purified water Remaining amount Total 100

Example 5. Body Lotion

[0075] A body lotion composition having a composition as shown in Table 6 below was prepared.

TABLE-US-00006 Ingredient name Content (Wt%) Ingredient name Content (Wt%) Glycerin 8.000 Inulin 0.001 Caprylic/Capric triglycerides 2.000 Beta-glucan 0.001 Cetyl ethylhexanoate 2.000 Maltodextrin 0.001 Glyceryl stearate 2.000 Beer-derived yeast extract 0.001 Cetearyl alcohol 1.000 Truffle-derived yeast extract 0.001 Acrylate/C10-30 alkyl acrylate crosspolymer 0.100 Lactobacillus ferment lysate 0.001 Disodium EDTA 0.020 Bifidobacterium ferment lysate 0.001 pH adjuster Appropriate amount Spices Appropriate amount Preservative Appropriate amount Purified water Remaining amount Total 100

Experimental Example 1. Identification of the Effect on the Growth of Beneficial Bacteria

[0076] In order to identify the effect of complexes of Preparation Examples on the growth of beneficial bacteria, Staphylococcus epidermidis (S. epidermidis) was selected as beneficial bacteria and Staphylococcus aureus (S. aureus) as harmful bacteria. Before use, the bacteria were stored at -80° C., a plurality of 100 mL tryptic soy broth (TSB) media was prepared, and 500 .Math.l of each bacterium stock was inoculated for each medium. After rotary culture at a rate of 210 rpm at 37° C. for 17 hours, 4 mL of the culture medium was collected to be inoculated into 40 mL of TSB, followed by subculture at a rate of 210 rpm at 37° C. for 8 hours.

[0077] After 8 hours, the optical density (O.D.) value was adjusted to 0.65, complexes prepared according to Preparation Examples 1 to 7 were inoculated at a concentration of 1%, respectively, and initial optical density values were measured. Thereafter, the optical density was measured after rotary culture at a rate of 210 rpm at 37° C. for 16 hours. The difference between the optical density measurement value and the initial measurement value after 16 hours was standardized for each medium by dividing by the initial measurement value for evaluation.

[0078] Used as comparative examples were inulin, beta-glucan, maltodextrin, the beer-derived yeast extract, the truffle-derived yeast extract, the Lactobacillus ferment lysate, and the Bifidobacterium ferment lysate, each inoculated at a concentration of 1%. An untreated group that was treated with none was used as a control group.

[0079] The inoculation results of the Preparation Examples and Comparative Examples were compared to the untreated group, and the average degree of increase or decrease of beneficial bacteria and harmful bacteria was converted into percentages. The results are shown in Table 7 below.

TABLE-US-00007 Classification Beneficial bacteria (S. epidermidis) Harmful bacteria (S. aureus) Lactobacillus ferment lysate -9.90 21.42 Bifidobacterium ferment lysate -4.69 26.00 Beer-derived yeast extract 2.48 16.37 Truffle-derived yeast extract 3.39 14.95 Inulin 0.29 -0.84 Beta-glucan -0.26 -0.78 Maltodextrin 0.14 1.43 Preparation Example 1 5.51 -2.46 Preparation Example 2 4.79 -2.88 Preparation Example 3 3.88 -2.31 Preparation Example 4 7.18 -2.49 Preparation Example 5 6.07 -2.71 Preparation Example 6 5.99 -2.19 Preparation Example 7 15.54 -7.47

[0080] As shown in Table 7, although inulin, beta-glucan, and maltodextrin are polysaccharides with prebiotic characteristics, no significant effect was shown alone on the increase in beneficial bacteria and reduction in harmful bacteria. The ferment lysate with probiotic characteristics alone rather showed effects of decreasing beneficial bacteria while reducing harmful bacteria.

[0081] In contrast, the complexes according to Preparation Examples 1 to 7 of the present disclosure showed significantly outstanding effects in increasing beneficial bacteria and reducing harmful bacteria compared to Comparative Examples in which the polysaccharides, the yeast extract, or the ferment lysate were used alone. When the polysaccharides as prebiotics as well as the yeast extract and the ferment lysate as probiotics were used together, it was found that there was a synergistic effect in the increase in beneficial bacteria and the decrease in harmful bacteria. In particular, it was observed that Preparation Example 7 showed the most outstanding effect on the increase in beneficial bacteria and the increase in harmful bacteria compared to other Preparation Examples.

Experimental Example 2. Identification of Activity of Dermal Papilla Cells

[0082] In order to identify the effect of the complexes of Preparation Examples on dermal papilla cells, human dermal papilla cells (Promocell, C-12071) in the 4-8 passage were used as a cell line, and follicle dermal papilla cell growth media (Promocell, C-26501) were used as an initial culture medium.

[0083] Cells were seeded at a level of 3,000 to 6,000 cells per well in a 96-well plate, and cultured in an incubator at 37° C. in the presence of 5% carbon dioxide for 24 hours. Thereafter, the culture medium was replaced with DMEM medium supplemented with 0.1% fetal bovine serum (FBS) and cultured in an incubator at 37° C. in the presence of 5% carbon dioxide for 24 hours. Thereafter, the culture medium was replaced with DMEM supplemented with 0.1% FBS treated with the complexes according to Preparation Examples 1 to 7 by each concentration, followed by culture in an incubator at 37° C. in the presence of 5% carbon dioxide for 48 hours. After treating 10 .Math.l of CCK-8 solutions per well, culture was performed at 37° C. for 1 hour, and optical density was measured at 450 nm.

[0084] The difference between the optical density measurement value and the initial measurement value after 48 hours for each well was standardized by dividing the difference by the initial measurement value for evaluation, and the average value was shown in Table 8 below. 1 .Math.M minoxidil was treated as a control group. For a significant difference, p-value was derived by assuming equal variance (significant difference p<0.05).

TABLE-US-00008 Concentration Average Preparation Example 1 1 ppm 5.8 10 ppm 7.7 Preparation Example 2 1 ppm 5.6 10 ppm 7.1 Preparation Example 3 1 ppm 5.2 10 ppm 8.1 Preparation Example 4 1 ppm 4.8 10 ppm 7.7 Preparation Example 5 1 ppm 6.1 10 ppm 10.2 Preparation Example 6 1 ppm 5.9 10 ppm 8.8 Preparation Example 7 1 ppm 16.9 10 ppm 21.5 Minoxidil 1 .Math.M 44.2

[0085] As shown in Table 8, it was found that the complexes according to Preparation Examples 1 to 7 of an embodiment of the present disclosure had a synergistic effect on dermal papilla cell activity. In particular, it was found that Preparation Example 7 showed the most outstanding effect on the increase in dermal papilla cell activity compared to other Preparation Examples.

Experimental Example 3. Identification of Suppression of Inflammatory Factors

[0086] In order to identify the effect of the complexes of Preparation Examples on the production of NO as an inflammatory factor in cells, a mouse Raw 264.7 cell line was used, and a culture medium whose final pH was adjusted to 8.5 through addition of NaOH to Complete RPMI (RPMI:FBS:antibiotic = 10:1:0.1) was used as an initial culture medium.

[0087] When the Raw 264.7 cells appeared at the top of the culture medium after culture at 37° C. in the presence of 5% carbon dioxide for 24 hours, the cells were seeded in a 24-well plate in which a DMEM culture was prepared, followed by culture at 37° C. in the presence of 5% carbon dioxide for 24 hours. Thereafter, treatment was performed after diluting the complexes according to Preparation Examples 1 to 7 by the following concentrations while replacing the medium with a serum-free DMEM culture medium, followed by additional treatment of 1 .Math.g/mL of LPS. After culture at 37° C. in the presence of 5% carbon dioxide for 24 hours, the supernatant was mixed with Griess reagent (Sigma-Aldrich) in a ratio of 1:1 to evaluate the ability to inhibit NO production with absorbance, and the average value was shown in Table 9 below.

[0088] NO production inhibition ability (%) = {1 - (NO production when compound is added / NO production in untreated group)} × 100

[0089] 20 .Math.g/mL of L-NMMA1 (N.sup.G-Methyl-L-arginine acetate salt), an NO inhibitor, was used as a control group.

TABLE-US-00009 Concentration Average Preparation Example 1 1% 2.2 7% 10.8 Preparation Example 2 1% 3.1 7% 7.5 Preparation Example 3 1% 3.5 7% 8.1 Preparation Example 4 1% 2.8 7% 9.4 Preparation Example 5 1% 3.3 7% 8.7 Preparation Example 6 1% 3.3 7% 7.5 Preparation Example 7 1% 5.7 7% 23.3 L-NMMA1 20 .Math.g/mL 52.1

[0090] As shown in Table 9, it was found that the complexes according to Preparation Examples 1 to 7 of an embodiment of the present disclosure had the ability to inhibit NO production as an inflammatory factor in cells. In particular, it was found that Preparation Example 7 showed the most excellent NO production inhibitory ability compared to other Preparation Examples.

Experimental Example 4. Identification of Collagen Biosynthesis Rate

[0091] The effect of the complexes of Preparation Examples on the collagen biosynthesis rate in the human skin fibroblasts was identified. Skin fibroblasts were cultured in complete DMEM culture at 37° C. in the presence of 5% carbon dioxide for 72 hours. After seeding in a 24-well plate, culture was performed at 37° C. in the presence of 5% carbon dioxide for 24 hours. After treating the complexes according to Preparation Examples and culturing at 37° C. in the presence of 5% carbon dioxide for 48 hours, only the supernatant was separated, and the collagen synthesis effect was observed by absorbance using a procollagen type I C-peptide (PIP) EIA kit. The increase rate of collagen biosynthesis was evaluated as follows, and the average value was shown in Table 10 below:

[0092] Collagen biosynthesis increase rate (%) = {(Absorbance of complex treated group -Absorbance of untreated group) / Absorbance of untreated group} × 100

[0093] 10 ng/mL of TGF-β, a cytokine, was used as a control group.

TABLE-US-00010 Concentration Average Preparation Example 1 1% 8.7 7% 10.8 Preparation Example 2 1% 8.6 7% 11.5 Preparation Example 3 1% 7.1 7% 10.8 Preparation Example 4 1% 9.5 7% 12.2 Preparation Example 5 1% 10.3 7% 15.4 Preparation Example 6 1% 8.5 7% 11.1 Preparation Example 7 1% 15.0 7% 35.5 TGF-β 10 ng/mL 36.6

[0094] As shown in Table 10, it was found that the complexes according to Preparation Examples 1 to 7 of an embodiment of the present disclosure had an effect of increasing collagen biosynthesis in fibroblasts. In particular, it was found that Preparation Example 7 showed the most excellent effect on the increase in collagen biosynthesis in fibroblasts compared to other Preparation Examples.

Experimental Example 5. Checking Whether Scalp Oil is Improved

[0095] The scalp oil improvement effect when the hair shampoo composition according to Example 1-1 was used was identified. As test subjects, 22 adult women aged 20 to 50 years were chosen, whose scalp was selected as the test site. The test subjects were not allowed to use other products such as shampoos, treatments, and hair essences and get hair dyed or permed during the experiment.

[0096] The experiment was performed after allowing the test subject to take a rest for 30 minutes in a constant temperature and humidity room at 22±2° C. and 50±5% humidity, and all experiments were performed in the constant temperature and humidity room. After wetting the hair and scalp of the test subject sufficiently with lukewarm water, the same amount of the hair shampoo composition according to Example 1-1 was evenly applied to the hair and scalp. After massage, rinsing was followed thoroughly with running water.

[0097] The evaluation for scalp oil before and after the use of the hair shampoo was performed using a Sebumeter (SKIN-O-MAT, Cosmomed GmbH) and a video microscope (Kong PC Camera, Bomtech). A probe cassette with an oil adsorption tape attached was placed on the top of the head of all test subjects, and the oil was sufficiently absorbed by brining the cassette in contact with the same pressure for 30 seconds. Then, the cassette was inserted into a main body of the Sebumeter to measure the amount of sebum. In addition, under the same lighting condition, the top of the head of all test subjects was observed at 300 times magnification using a video microscope.

[0098] Hereinafter, the analysis was conducted using SPSS 17.0 for Windows for statistical processing in this experiment. Table 11 below is a result showing the amount of sebum (.Math.g/cm.sup.2) before use and after a single use of the composition of Example 1-1 (the maximum amount of sebum measurable with a Sebumeter is 350 .Math.g/cm.sup.2).

TABLE-US-00011 Before use Immediately after a single use Average 200.41 32.05 Standard Deviation 37.03 14.87

[0099] Table 12 below shows the results of paired t-test analysis (*p<0.05, **p<0.01, ***p<0.001) for analyzing the sebum amount improvement rate (%) and significant change after a single use of the composition of Example 1-1: Improvement rate (%) = {(Measured value after 1 time use - Measured value before use) / Measured value before use} × 100

TABLE-US-00012 Sebum improvement rate p-value Immediately after a single use 84.01 0.001 ***

[0100] In addition, scalp condition of the individual test subject and changes in the scalp condition before use and after a single use of the composition of Example 1-1 were surveyed, and the results were shown in Tables 13 and 14 below, respectively (N = Total frequency = Number of subjects = 22).

TABLE-US-00013 Question Frequency Percentage (%) The scalp is clean with no wastes or foreign substances. Not at all 2 9.1 No 19 86.4 Indifferent 1 4.5 Yes 0 0.0 Excellent 0 0.0 The scalp is fresh without excessive sebum secretion. Not at all 2 9.1 No 19 86.4 Indifferent 1 4.5 Yes 0 0.0 Excellent 0 0.0 Total 22 100.0

TABLE-US-00014 Question Frequency Percentage (%) Wastes and foreign substances on the scalp seem to be Not at all 0 0.0 No 0 0.0 Indifferent 1 4.5 thoroughly cleaned. Yes 20 91.0 Excellent 1 4.5 The scalp seems to become fresh due to reduced amount of excess sebum. Not at all 0 0.0 No 0 0.0 Indifferent 2 9.1 Yes 16 72.7 Excellent 4 18.2 Total 22 100.0

[0101] Additionally, a survey was conducted by asking whether there are any adverse reaction to the scalp and hair when using the composition of Examples. The results were shown in Table 15 below (0: none, 1: mild, 2: moderate, 3: severe).

TABLE-US-00015 Adverse reaction Immediately after a single use Adverse reaction Immediately after a single use Erythema (redness) 0 Stinging (pain) 0 Edema (swelling) 0 Burning sensation 0 Scale (keratin) 0 Stiffness 0 Itchiness 0 Tingling 0

[0102] As shown in Tables 11 and 12, after a single application of the hair shampoo according to Example 1-1 of an embodiment of the present disclosure, the amount of sebum was improved by 80% or higher compared to before, showing that the scalp oil improvement effect was excellent. These results were consistent with the results of the questionnaire on the test subjects shown in Tables 13 to 15 in that more than 90% of the test subjects answered that there were cleaning and sebum removal effects without side effects.

[0103] FIGS. 1 to 3 are photographs showing enlarged observation results (A) before application and (B) after a single application of the hair shampoo according to Example 1-1 of an embodiment of the present disclosure for three test subjects, showing that the cleaning and sebum removal effects were excellent.

Experimental Example 6. Checking Whether the Scalp Flora Is Improved

[0104] The effect of improving the scalp flora was identified when the hair shampoo composition according to Example 1, the hair treatment composition according to Example 2, and the hair tonic composition according to Example 3 were used. As the test subjects, 24 adult women with an average age of 45 were chosen, whose scalp was selected as the test site. The test subjects were not allowed to use other products such as shampoos, treatments, and hair essences and get hair dyed or permed during the experiment.

[0105] The experiment was performed after allowing the test subjects to take a rest for 30 minutes in a constant temperature and humidity room at 20 to 25° C. and 40 to 60% humidity, and all experiments were performed in the constant temperature and humidity room. After the hair and scalp of the test subjects were sufficiently wet with lukewarm water, the hair shampoo composition according to Examples 1-1 and 1-2 and the hair treatment composition according to Example 2 were evenly applied to the hair and scalp in the same amount once a day, respectively. After massage, the test subjects were directed to rinse thoroughly with running water. In addition, the test subjects were directed to evenly apply the same amount of the hair tonic composition according to Example 3 to the scalp once a day. After massage, the applied hair tonic composition was left to be dried without rinsing.

[0106] After the experiment period, without applying shampoo and water to the hair and scalp for a day, the test subjects were allowed to take a rest for 30 minutes in the constant temperature and humidity room. Microorganisms on the scalp were collected by swabbing the scalp with the cotton swab up and down 40 times while the middle line of the head is fixed so as to compare and analyze with the microorganisms collected before the experiment.

[0107] Hereinafter, the analysis was conducted using SPSS for statistical processing in this experiment. As a result of the analysis, it was determined that there was an improvement effect when the significance probability is greater than 0.05 (p<0.05) in the 95% confidence interval. As for the statistical analysis, the analysis was conducted using paired samples t-test when a parametric method was applied and Wilcoxon signed rank test when a nonparametric method was applied. Table 16 shows results of analysis of variance (ANOVA) at the genus level before and after use of the composition according to Examples.

TABLE-US-00016 Genus p value Before use 1 week after use 2 weeks after use Aspergillus p < 0.01 2.21 2.01 0.78 Fusarium p < 0.01 1.58 1.49 0.28 Penicillium p < 0.01 1.12 0.99 0.21 Cladosporium p < 0.01 1.03 0.98 0.19 Mucor p < 0.01 0.66 0.71 0.2 Alternaria p < 0.01 0.6 0.56 0.13 Kluyveromyces p < 0.01 0.66 0.54 0.06 Clitocybe p < 0.01 0.6 0.57 0.032 Sarocladium p < 0.01 0.53 0.55 0

[0108] After 2 weeks of using the composition according to Example, it was determined that the flora of 9 genera existing on the scalp was statistically significantly changed. As shown in Table 16, from 1 week after using the compositions according to Examples 1 to 3 of an embodiment of the present disclosure, the genus Saccharomyces, known to contribute to scalp moisturization and elasticity improvement, increased statistically significantly. In the genera of Aspergillus, Fusarium and Penicillium, known to cause mycoses or dermatitis, it was found that the flora was statistically significantly reduced after 2 weeks of use, meaning that the flora was statistically significantly improved in the scalp.

Experimental Example 7. Checking Whether Skin Flora is Improved

[0109] The skin flora improvement effect was identified when the body wash composition according to Example 4 and the body lotion composition according to Example 5 were used. As the test subjects, 22 adult women aged 20 to 60 years were chosen, whose skin of the forearm was selected as the test site. The test subjects were not allowed to use other body products during the experimental period.

[0110] The experiment was performed after the test subjects were allowed to take a rest for 30 minutes in a constant temperature and humidity room at 20 to 25° C. and 40 to 60% humidity without washing the test site for more than 8 hours, and all experiments were carried out in the constant temperature and humidity room. The skin of the forearm of the test subjects was wet with lukewarm water, washed with the body wash composition according to Example 4, and evenly applied with the body lotion composition according to Example 5.

[0111] After the experiment period, the microorganisms were collected by swabbing the skin of the forearm with the cotton swab up and down 40 times, and compared and analyzed with the microorganisms collected before the experiment. Statistical processing was performed in the same manner as in Experimental Example 6 described above. Table 17 shows the results of analysis of variance (ANOVA) at the genus level before and after the use of the composition according to the Example.

TABLE-US-00017 Genus p value Before use 1 week after use 2 weeks after use Ehrlichia p < 0.001 7.43 7.54 2.62 Sphingomonas p < 0.001 3.19 3.22 2.29 Pseudomonas p < 0.001 1.56 1.5 3.31 Acetobacter p < 0.001 2.65 2.7 0.95 Cutibacterium p < 0.001 1.22 1.17 3.07 Streptococcus p < 0.001 1.13 0.96 1.93 Akkermansia p < 0.001 1.55 1.56 0.85 Bacteroides p < 0.001 1.37 1.41 0.69 Staphylococcus p < 0.001 0.72 0.72 1.81 Enterococcus p < 0.001 0.93 0.92 0.80

[0112] After 2 weeks of using the composition according to the Example, it was found that the flora of 10 genera on the skin was statistically significantly changed. As shown in Table 17, the Staphylococcus and Cutibacterium genera increased statistically significantly after 2 weeks of use of the compositions according to Examples 4 and 5 of an embodiment of the present disclosure, and Ehrlichia and Sphingomonas genera, known to cause infections, were statistically significantly reduced after 2 weeks of use. Thus, the flora in the skin was statistically significantly improved. In addition, as a result of measuring the diversity of the flora (β-diversity), a statistically significant increase was observed, determining that the flora in the skin was statistically significantly improved.

[0113] The present disclosure is not limited to the examples described above, and a combination of the examples or a combination of at least one of the examples and a known technology may be included as another example.

[0114] Although the present disclosure has been described in detail through specific examples, this is for the purpose of describing the present disclosure in detail, and the present disclosure is not limited thereto. It will be apparent that modifications or improvements are possible by those of ordinary skill in the art within the technical spirit of the present disclosure.

[0115] All simple modifications or changes of the present disclosure fall within the scope of the present disclosure, and the specific protection scope of the present disclosure will become apparent by the appended claims.