COMPOSITION FOR PROMOTING HAIR GROWTH AND/OR INHIBITING HAIR LOSS

Abstract

The present application relates to a composition including a herb extract and/or an effective compound as an active ingredient for promoting hair growth and/or preventing, alleviating, or treating hair loss.

Claims

1-9. (canceled)

10. A method for promoting hair growth, or preventing, alleviating or treating hair loss, comprising a step of administering an effective dose of at least one selected from the group consisting of sulforaphane, indole-3-carbionol, beta-sitosterol, and salts thereof to a subject in need of promoting hair growth, or preventing, alleviating or treating hair loss.

11. The method according to claim 10, characterized in administering an effective dose of (1) sulforaphane or salts thereof; and (2) beta-sitosterol or salts thereof.

12. The method according to claim 11, characterized in further administering an effective dose of indole-3-carbinol or salts thereof.

13. The method according to claim 10, wherein a ratio of sulforaphane, indole-3-carbinol and β-sitosterol administered in the method is 10:0.1 to 10:0.1 to 10 (so far, sulforaphane weight:indole-3-carbinol weight:β-sitosterol weight).

14. The method according to claim 10, wherein a ratio of sulforaphane, indole-3-carbinol and β-sitosterol administered in the method is 10:0.1 to 5:0.1 to 5 (so far, sulforaphane weight:indole-3-carbinol weight:β-sitosterol weight).

15. The method according to claim 10, wherein the step of administering is performed by oral administration.

16. A method for promoting hair growth, or preventing, alleviating or treating hair loss, comprising a step of administering an effective dose of a broccoli (Brassica oleracea var. italica) extract, an avocado (Persea americana) extract or a mixture thereof as an active ingredient to a subject in need of promoting hair growth, or preventing, alleviating or treating hair loss, wherein the broccoli (Brassica oleracea var. italica) extract is obtained by extracting the broccoli with water.

17. The method according to claim 16, characterized in administering an effective dose of (1) a broccoli extract; or (2) a mixture of a broccoli extract and an avocado extract.

18. The method according to claim 16, wherein the broccoli extract is obtained by extracting the broccoli with water at 50 to 90° C.

19. The method according to claim 16, wherein the avocado extract is obtained by extracting the avocado with petroleum ether.

20. The method according to claim 16, characterized in that the broccoli extract comprises sulforaphane or salts thereof.

21. The method according to claim 20, characterized in that the broccoli extract further comprises beta-sitosterol or salts thereof, indole-3-carbinol or salts thereof, or a mixture thereof.

22. The method according to claim 20, characterized in that the broccoli extract comprises sulforaphane or salts thereof, beta-sitosterol or salts thereof, and indole-3-carbinol or salts thereof.

23. The method according to claim 16, wherein the step of administering is performed by oral administration.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0076] FIG. 1a is a graph showing the testosterone concentration in serum of the rat administered with the broccoli extract, avocado extract or mixture thereof according to one example.

[0077] FIG. 1b is a graph showing the dihydrotestosterone (DHT) concentration in serum of the rat administered with the broccoli extract, avocado extract or mixture thereof according to one example.

[0078] FIG. 2 is photographs of staining the skin tissue of the rat administered with the broccoli extract, avocado extract or mixture thereof according to one example.

[0079] FIG. 3 is a graph showing the length of the hair follicle in the skin of the rat administered with the broccoli extract, avocado extract or mixture thereof according to one example.

[0080] FIG. 4a is a graph showing the testosterone concentration in serum of the rat administered with sulforaphane, indole-3-carbionol and beta-sitosterol each or in combination.

[0081] FIG. 4b is a graph showing the dihydrotestosterone concentration in serum of the rat administered with sulforaphane, indole-3-carbionol and beta-sitosterol each or in combination.

[0082] FIG. 5 is a graph showing the length of the hair follicle in the skin of the rat administered with sulforaphane, indole-3-carbionol and beta-sitosterol each or in combination.

MODE FOR INVENTION

[0083] Hereinafter, the present invention will be described in more detail by the following examples. However, they are intended to illustrate the present invention only, but the scope of the present invention is not limited by these examples.

Example 1: Preparation of Extract

[0084] 1.1. Preparation of Broccoli Extract

[0085] Broccoli (Brassica oleracea var. italica) was washed using clean water and then dried sufficiently. To the powder 100 g obtained by crushing the dried broccoli, 10 volume times (500 ml) of water or ethanol (10 to 100% (v/v) ethanol (spirit)) was added, and after extracting at 70° C. for 3 hours or more three times, the filtrate filtered through a 1 μm (micrometer) filter was concentrated with heating until it reached 10% (w/w) of the original weight. The obtained concentrate was continuously concentrated, dried completely, and then powdered to prepare a broccoli extract powder. Among them, broccoli water extract powder (hereinafter, referred to as BE) using water as an extraction solvent was used for subsequent tests.

[0086] 1.2. Preparation of Avocado Extract

[0087] Avocado (Persea americana) was sterilized in chlorinated water for 15 minutes. The avocado flesh was separated and homogenized after adding a 1% (v/v) citric acid solution. The homogenized solution was lyophilized and powdered and stored frozen for 1 week before extraction. The prepared freeze-dried powder 100 g was dissolved in 500 mL petroleum ether (CAS No. 101316-46-5) and extracted with a Soxhlet extractor to obtain an oily extract. The extracted oil was powdered to prepare an avocado extract powder (hereinafter, referred to as AE).

Example 2: Confirmation of Male Hormone Reducing Effect of Broccoli Extract and/or Avocado Extract

[0088] The effect of reducing testosterone and dihydrotestosterone (hereinafter, referred to as DHT) of the broccoli extract and/or avocado extract prepared in Example 1 was confirmed by collecting plasma of male wistar albino rats and using a testosterone and DHT ELISA kit.

Example 2-1. Breeding of Experimental Animals

[0089] Male wistar albino rats weight 180 to 240 g, February to March age were bred under the conditions of a constant light-dark cycle in which light was applied from 8 am to 8 pm, a temperature of 22 to 25° C., and a relative humidity of 60%. For the feed of experimental animals, a general pellet dry feed was used, and feed and water were allowed to be consumed at all times.

Example 2-2. Measurement of Concentration of Testosterone and DHT after Extract Administration

[0090] To the experimental animals bred in Example 2-1, testosterone solution 0.20 obtained by diluting 100 mg of commercially available testosterone (SUSTANON®) in arachis oil 1000 was subcutaneously injected daily for a total of 21 days.

[0091] In addition, the broccoli extract and/or avocado extract prepared in Example 1 were orally administered for a total of 21 days daily so as to be a dose of 100 mg per 1 kg of the body weight of each experimental animal (in case of coadministration group, broccoli extract 67 mg/kg+avocado extract 33 mg/kg).

[0092] The experimental animal groups treated with the testosterone solution, broccoli extract, avocado extract or mixture of broccoli extract and avocado extract were arranged in Table 1 below.

TABLE-US-00001 TABLE 1 Extract mixture Testosterone Broccoli extract Avocado extract (BE 67 mg/kg + AE (T; for 21 days) (BE 100 mg/kg) (AE 100 mg/kg) 33 mg/kg) Normal group — — — — (Group 1) Negative control ∘ group (Group 2) Experimental ∘ ∘ group (Group 3) Experimental ∘ ∘ group (Group 4) Experimental ∘ ∘ group (Group 5)

[0093] For 21 days, the testosterone solution and broccoli extract, avocado extract or broccoli and avocado extract were administered, and on the next day, before skin samples were collected, plasma from rats was extracted and the concentration of testosterone and DHT were measured using a testosterone and DHT ELISA kit (Arigo Biolaboratories, Taiwan) according to the manufacturer's instructions.

[0094] The result of measuring the testosterone and DHT in plasma of the normal group, negative control group and experimental groups was shown in FIG. 1a and FIG. 1b, respectively. As shown in FIG. 1a and FIG. 1b, in case of the negative control group administered only with the testosterone solution, the concentration of testosterone and DHT in plasma was largely increased, compared to the normal group. On the other hand, in case of experimental groups administered with the broccoli extract and/or avocado extract (Groups 3, 4, 5), the concentration of testosterone and DHT in plasma was reduced, and among them, in particular, in case of the experimental group administered with the mixture of the broccoli extract and avocado extract (Group 5), the concentration of testosterone and DHT in plasma was significantly reduced at a level equal to the normal group (Group 1).

Example 3: Confirmation of Effect on Hair Follicles of Broccoli Extract or Avocado Extract

Example 3-1. Shape Change of Hair Follicles (Stained Photograph)

[0095] By staining the skin tissue of experimental animals, the shape change of hair follicles was investigated. To the test animals prepared in Example 2-1, the testosterone solution and broccoli extract, avocado extract or mixture thereof was administered for 21 days, and on the next day, the skin of the dorsal area of experimental animals was removed using a hair removing drug (Hair removing cream Veet®), and then it was incised and fixed with 10% (v/v) formalin. After 24 hours, the skin sample was soaked in paraffin wax and sectioned to a uniform thickness of 10 μm, and hair follicles were stained with haematoxylin and eosin. Slides were observed under a microscope and images were recorded using standard software (Leica, Germany).

[0096] The stained photograph of the skin tissue of the observed normal group, negative control group and experimental groups was shown in FIG. 2. As shown in FIG. 2, in case of the negative control group administered only with the testosterone solution, many hair follicles in the telogen stage were found. On the other hand, in case of the experimental groups (Groups 3, 4, 5) administered with the broccoli extract and/or avocado extract, the length of hair follicles was increased, and the length of hair follicles was found to be similar to that of the normal group (Group 1).

Example 3-2. Measurement of Density of Hair Follicles

[0097] The density of hair follicles was measured in the skin of the normal group, negative control group and experimental group and shown in Table 2 below. The density of hair follicles was measured by counting the number of hair follicles in a unit area (1 mm.sup.2). The obtained result was shown in Table 2.

TABLE-US-00002 TABLE 2 Rate of change compared Hair follicle density to normal group Normal group 1.31 ± 0.021  — (Group 1) Negative control 0.91 ± 0.069  −30.53% group (Group 2) Experimental 2.40 ± 0.053* +83.21% group (Group 3) Experimental 2.09 ± 0.032* +59.54% group (Group 4) Experimental 2.61 ± 0.057* +99.24% group (Group 5) (In Table 2, Values are mean ± SEM, n = 15, *p < 0.05 as compared to negative control)

[0098] The rate of change compared to the normal group described in Table 2 was calculated according to Equation 1 below.

Rate of change compared to normal group=(hair follicle density of negative control group or experimental group−hair follicle density of normal group)/hair follicle density of normal group  (Equation 1)

[0099] As shown in Table 2 above, in case of the negative control group administered only with the testosterone solution, the hair follicle density was reduced by about 31% compared to the normal group. On the other hand, in case of the experimental groups (Groups 3, 4, 5) administered with the broccoli extract and/or avocado extract, they exhibited an excellent effect that the hair follicle density was increased by about 83% (broccoli extract), about 60% (avocado extract) or about 99% (mixture of broccoli extract and avocado extract), respectively, compared to the normal group.

Example 3-3. Comparison of Condition of Hair Follicles

[0100] The ratio of the anagen stage and telogen stage of hair follicles in the skin of the normal group, negative control group and experimental groups was calculated and shown in Table 3 below.

TABLE-US-00003 TABLE 3 Rate of change Anagen/telogen compared to Anagen Telogen ratio normal group Normal group 66.1 33.9 1.949853 — (Group 1) Negative control 14.6 85.4 0.17096 −91.23% group (Group 2) Experimental 60.4 39.6 1.525253* −21.78% group (Group 3) Experimental 51.2 48.8 1.04918* −46.19% group (Group 4) Experimental 65.6 34.4 1.906977* −2.20% group (Group 5) *p < 0.05 as compared to negative control

[0101] The rate of change compared to the normal group in Table 3 was calculated according to Equation 2 below.

Rate of change compared to normal group=(anagen/telogen ratio of negative control group or experimental group−anagen/telogen ratio of normal group)/anagen/telogen ratio of normal group  (Equation 2)

[0102] As shown in Table 3, in case of the negative control group administered only with the testosterone solution, the hair follicles in the anagen stage were reduced and the hair follicles in the telogen stage were increased, and therefore, the anagen/telogen ratio was reduced by about 91% compared to the normal group. On the other hand, in case of all the experimental groups (Groups 3, 4, 5) administered with the broccoli extract and/or avocado extract, compared to the negative control group, the hair follicles in the anagen stage were increased and the hair follicles of telogen stage were reduced. Among them, in particular, in case of the experimental group administered with the mixture of the broccoli extract and avocado extract (Group 5), compared to the negative control group, the hair follicles in the anagen stage were largely increased and the hair follicles in the telogen stage were significantly reduced, and the anagen/telogen ratio was shown at a similar level to the normal group.

Example 3-4. Comparison of Length of Hair Follicles

[0103] The length of hair follicles was measured in the skin of the normal group, negative control group and experimental groups and shown in FIG. 3. For the length of hair follicles, the length of stained hair follicles photographed in Example 3-1 was measured using a standard software (Leica, Germany), and the result was shown in FIG. 3.

[0104] As shown in FIG. 3, in case of the negative control group administered only with the testosterone solution, the length of hair follicles was largely reduced, compared to the normal group. On the other hand, in case of the experimental groups administered the broccoli extract and/or avocado extract (Groups 3, 4, 5), compared to the negative control group, the length of hair follicles was increased. Among them, in particular, in case of the experimental group administered with the mixture of the broccoli extract and avocado extract, compared to the negative control group, the length of hair follicles was significantly increased, and it exhibited an excellent effect that the length of hair follicles was increased, compared to the normal group.

Example 3-5. Confirmation of Effect Depending on Broccoli Extraction Solvent

[0105] A broccoli water extract which broccoli was extracted with water according to Example 1-1 was obtained. As a comparative group, a broccoli extract was obtained in the same manner as Example 1-1, except that the extraction solvent was extracted with an aqueous ethanol solution of various concentrations (10%, 30%, 50%, 70%, 100%).

[0106] The sulforaphane (SFN) content in the broccoli water extract (DW) and comparative group (EtOH; ethanol extracts at various concentrations) was measured, and the result was shown in Table 4 below.

TABLE-US-00004 TABLE 4 Extraction SFN content solvent (ug/ml) 100% ND EtOH 70% EtOH ND 50% EtOH ND 30% EtOH 1.7 10% EtOH 5.2 DW 61.41 (ND: not detected)

[0107] As shown in Table 4 above, it was confirmed that all the extracts obtained by extracting broccoli with water or aqueous ethanol solution at various concentrations (30% and 10%) contained sulforaphane. Among them, it was confirmed that the sulforaphane content of the extract obtained by extracting broccoli with water was particularly high.

[0108] By the same method as Example 3-2, the broccoli water extract and broccoli ethanol extract were administered to the experimental animals prepared in Example 2-1 and the density of hair follicles in the skin of the experimental animals was measured and the result was shown in Table 5 below. The normal control group (control; normal group) is a group in which neither testosterone nor the extract is administered, and the negative control group (Testosterone; T) is a group in which only testosterone is administered.

TABLE-US-00005 TABLE 5 Extraction Hair follicle solvent density Control 1.25 ± 0.39 Testosterone 0.98 ± 0.23 T + 70% EtOH 0.99 ± 0.48 T + 30% EtOH  1.0 ± 0.65 T + 10% EtOH 1.14 ± 0.73 T + DW  1.93 ± 0.102

[0109] The hair follicle density was shown as mean±mean standard error. The rate of change compared to the normal group was calculated by the same method as Equation 1 above.

[0110] As shown in Table 5 above, it was confirmed that the hair follicle density reduced by testosterone was increased again, when the extract obtained by extracting broccoli with water or aqueous ethanol solution at various concentrations, compared to the negative control group administered only with the testosterone solution. In particular, it was confirmed that the hair follicle density was more significantly increased, when the extract obtained by extracting broccoli with water.

Example 4: Confirmation of Male Hormone Reducing Effect of Compounds

[0111] To the experimental animals bred in Example 2-1, testosterone solution 0.20 obtained by diluting 100 mg of commercially available testosterone (SUSTANON®) in arachis oil 1000 was subcutaneously injected daily for a total of 21 days, and according to Table 6 below, sulforaphane, indole-3-carbinol, beta-sitosterol or a mixture of the compounds was orally administered daily for 21 days at a dose of 10 mg/kg.

[0112] The experimental animal groups treated with the sulforaphane, indole-3-carbinol, beta-sitosterol or mixture of the compounds were arranged in Table 6 below.

TABLE-US-00006 TABLE 6 Mixture of compounds Indole-3- (SFN 8 mg/kg + Testosterone Sulforaphane carbinol I3C 1 mg/kg + (T; for 21 (SFN 10 (I3C 10 β-sitosterol β-sitosterol days) mg/kg) mg/kg) (10 mg/kg) 1 mg/kg) Normal group — — — — — (Group A) Negative control ∘ group (Group B) Experimental ∘ ∘ group (Group C) Experimental ∘ ∘ group (Group D) Experimental ∘ ∘ group (Group E) Experimental ∘ ∘ group (Group F)

[0113] For 21 days, sulforaphane, indole-3-carbinol, β-sitosterol or a mixture thereof was administered, and on the next day, plasma from rats was extracted and the concentration of testosterone and DHT were measured using a testosterone and DHT ELISA kit (Arigo Biolaboratories, Taiwan) (See Example 2-2).

[0114] The result of measuring the concentration of testosterone and DHT in plasma of the normal group (control), negative control group and experimental group was shown in FIG. 4a (testosterone) and FIG. 4b (DHT), respectively. As shown in FIG. 4a and FIG. 4b, in case of the negative control group administered only with the testosterone solution, the concentration of testosterone and DHT in plasma was largely increased, compared to the normal group. On the other hand, it could be seen that the concentration of testosterone and DHT in plasma was significantly reduced, in case of the experimental groups administered with sulforaphane, indole-3-carbinol and/or β-sitosterol (Groups C, D, E, F). Among then, in particular, in case of the experimental group administered with the mixture of mixing sulforaphane, indole-3-carbinol and β-sitosterol (Group F), the concentration of testosterone and DHT in plasma was largely reduced at the same level as the normal group (Group A).

Example 5: Confirmation of Effect on Hair Follicles of Compounds

Example 5-1. Measurement of Density of Hair Follicles

[0115] Referring to Example 3-2, the density of hair follicles was measured in the skin of the normal group, negative control group and experimental groups and shown in Table 7 below.

TABLE-US-00007 TABLE 7 Density of hair Rate of change compared follicles to normal group (%) Normal group 1.31 ± 0.021 — (Group A) Negative control 0.91 ± 0.069 −30.53% group (Group B) Experimental  1.93 ± 0.102* +47.33% group (Group C) Experimental 1.59 ± 0.74* +21.37% group (Group D) Experimental 2.195 ± 0.093* +67.56% group (Group E) Experimental  2.41 ± 0.064* +83.97% group (Group F) Values are mean ± SEM, n = 15, *p < 0.05 as compared to negative control

[0116] The density of hair follicles was shown as mean±mean standard error. The rate of change compared to the normal group was calculated by the same method as Equation 1 above.

[0117] As shown in Table 7 above, in case of the negative control group administered only with the testosterone solution, the density of hair follicles was reduced by about 31% compared to the normal group. On the other hand, the experimental groups administered with sulforaphane, indole-3-carbinol, β-sitosterol or a mixture thereof (Groups C, D, E, F) exhibited an excellent effect of increasing the density of hair follicles by about 47% (sulforaphane), about 21% (indole-3-carbinol), about 68% (β-sitosterol) or about 84% (mixture of sulforaphane, indole-3-carbinol and β-sitosterol), respectively, compared to the normal group.

Example 5-2. Comparison of Condition of Hair Follicles

[0118] Referring to Example 3-3, the ratio of the anagen stage and telogen stage of hair follicles was calculated in the skin of the normal group, negative control group and experimental groups and shown in Table 8 below.

TABLE-US-00008 TABLE 8 Rate of change Anagen/telogen compared to Anagen Telogen ratio normal group Normal group 66.1 33.9 1.94 — (Group A) Negative control 14.6 85.4 0.17 −91.23% group (Group B) Experimental 50.8 49.2 1.03* −47.05% group (Group C) Experimental 46.8 53.2 0.87* −54.88% group (Group D) Experimental 50.2 49.8 1.00* −48.30% group (Group E) Experimental 58.4 41.6 1.40* −28.00% group (Group F) *p < 0.05 as compared to negative control

[0119] The rate of change compared to the normal group was calculated by the same method as Equation 2.

[0120] As shown in Table 8 above, in case of the negative control group administered only with the testosterone solution, the hair follicles in the anagen stage were reduced and the hair follicles in the telogen stage were increased, and the anagen/telogen ratio was reduced by about 91% compared to the normal group. On the other hand, in case of the experimental groups administered with sulforaphane, indole-3-carbinol, β-sitosterol or a mixture thereof (Groups C, D, E, F), the hair follicles in the anagen stage were increased and the hair follicles in the telogen stage were reduced, compared to the negative control group. Among them, in particular, it could be confirmed that in case of the experimental group administered with the mixture of mixing testosterone and sulforaphane, indole-3-carbinol and β-sitosterol (Group 6), the hair follicles in the anagen stage were largely increased and the hair follicles in the telogen stage were significantly reduced, compared to the negative control group.

Example 5-3. Comparison of Length of Hair Follicles

[0121] Referring to Example 3-4, the length of the hair follicles was measured in the skin of the normal group, negative control group and experimental groups and shown in FIG. 5. As shown in FIG. 5, in case of the negative control group administered only with the testosterone solution, the length of hair follicles was largely reduced, compared to the normal group (control). On the other hand, in case of the experimental groups administered with sulforaphane, indole-3-carbinol, β-sitosterol or a mixture thereof (Groups C, D, E, F), the length of hair follicles was increased, compared to the negative control group. Among them, in particular, it could be confirmed that in case of the experimental group administered with the mixture of mixing testosterone and sulforaphane, indole-3-carbinol and β-sitosterol (Group F), the length of hair follicles was largely increased, compared to the negative control group.

Example 5-4. Confirmation of Effect According to Mixing Ratio of Compounds

[0122] To the experimental animals bred in Example 2-1, mixtures of mixing sulforaphane, indole-3-carbinol and β-sitosterol at various mixing ratios as Table 9 below by the same method as Example 4 were orally administered daily at a dose of 1 Omg/kg in total for 21 days. The normal control group (control; normal group) is a group in which neither testosterone nor the extract is administered, and the negative control group (Testosterone; T) is a group in which only testosterone is administered.

[0123] Referring to Example 3-2, the density of hair follicles was measured in the skin of the normal control group, negative control group and experimental groups and shown in Table 9 below.

TABLE-US-00009 TABLE 9 Rate of change Density of hair compared to normal Mixing ratio follicles group Control 1.25 ± 0.39  — Testosterone 0.98 ± 0.23  −21.6% T + 8:1:1 2.41 ± 0.064 +92.80% (SFN 8 mg/kg + I3C 1 mg/kg + S 1 mg/kg) T + 4:3:3 1.96 ± 0.031 +56.80% (SFN 4 mg/kg + I3C 3 mg/kg + S 3 mg/kg) T + 1:8:1 1.60 ± 0.039 +28.00% (SFN 1 mg/kg + I3C 8 mg/kg + S 1 mg/kg) T + 3:3:4 1.91 ± 0.052 +52.80% (SFN 3 mg/kg + I3C 3 mg/kg + S 4 mg/kg) T + 1:1:8 1.87 ± 0.071 +49.60% (SFN 1 mg/kg + I3C 1 mg/kg + S 8 mg/kg) (SFN: sulforaphane, I3C: indole-3-carbinol, S: beta-sitosterol)

[0124] The density of hair follicles was shown as mean±mean standard error. The rate of change compared to the normal group was calculated by the same method as Equation 1 above.

[0125] As shown in Table 9 above, in case of the negative control group administered only with the testosterone solution, the density of hair follicles was reduced by about 22% compared to the normal group. However, it was confirmed that in all cases of administering mixtures of mixing sulforaphane, indole-3-carbinol and β-sitosterol at various mixing ratios, the density of hair follicles was significantly increased, compared to the negative control group.

Example 5-5. Confirmation of Effect According to Administration Method of Compounds

[0126] To the experimental animals bred in Example 2-1, a mixture of mixing sulforaphane, indole-3-carbinol and β-sitosterol at a ratio of 8:1:1 by the same method as Example 4 was orally administered daily at a dose of 10 mg/kg (SFN 8 mg/kg+I3C 1 mg/kg+S 1 mg/kg) in total for 21 days. As a comparative group, the mixture was administered by the same method, and the administration method was a method of intraperitoneal administration (IP). The normal control group (control; normal group) is a group in which neither testosterone nor the extract is administered, and the negative control group (Testosterone; T) is a group in which only testosterone is administered.

[0127] Referring to Example 3-2, the density of hair follicles was measured in the skin of the normal control group, negative control group and experimental groups and shown in Table 10 below.

TABLE-US-00010 TABLE 10 Rate of change Density of hair compared to normal Administration method follicles group Control 1.25 ± 0.39  — Testosterone 0.98 ± 0.23  −21.6% T + Oral administration 2.41 ± 0.064 92.8% T + IP injection 1.37 ± 0.051 9.6%

[0128] As shown in Table 10 above, in case of the negative control group administered only with the testosterone solution, the density of hair follicles was reduced by about 22% compared to the normal group. It was confirmed that in case of all experimental groups in which the mixture was intraperitoneally administered (IP injection) or orally administered (oral administration), the density of hair follicles was increased, compared to the negative control group, and in particular, in the orally administered experimental group, the density of hair follicles was more significantly increased.

[0129] From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as all changed or modified forms derived from the meaning and scope of the claims described below and their equivalents rather than the detailed description.