Preparation method and use of artificial exosome complex
11559477 · 2023-01-24
Assignee
Inventors
Cpc classification
A61K8/84
HUMAN NECESSITIES
A61K8/735
HUMAN NECESSITIES
A61K2800/5922
HUMAN NECESSITIES
International classification
A61K47/36
HUMAN NECESSITIES
A61K47/18
HUMAN NECESSITIES
Abstract
A preparation method and use of an artificial exosome complex are provided. Raw materials for preparing the artificial exosome complex include linear polyethyleneimine (PEI) and hyaluronic acid (HA). The linear PEI can be obtained through the HA-PEI preparation process of the present disclosure, and most PEI is branched currently. According to the HA-PEI preparation method, HA-PEI with a specific molecular weight can be quantitatively prepared. The preparation process involves controllable operation steps, and thus can be used for large-scale industrial production. The prepared HA-PEI can promote the absorption of an encapsulated active substance by deep skin cells through the ligand activity of HA and the membrane permeability of linear PEI.
Claims
1. A preparation method of an artificial exosome complex, wherein raw materials for preparing the artificial exosome complex comprise linear polyethyleneimine (PEI) and hyaluronic acid (HA), and the preparation method comprises the following steps: a. dissolving 45 g of poly(2-ethyl-2-oxazoline) in 200 mL of a 30% sulfuric acid aqueous solution to obtain a reaction solution, and setting up a heating reflux device; b. heating the reaction solution to allow a reaction at reflux, and continuously detecting a pH of a distilled solution to determine propionic acid (a reaction by-product) until there is no propionic acid in the distilled solution; and stopping the reaction when the pH of the distilled solution is 6.0 to 7.0; wherein the propionic acid forms an azeotrope with water, and the reaction takes about 7 d; c. after the reaction was stopped, cooling a resulting reaction system to 0° C., and starting clockwise one-way mechanical stirring at a speed of 300 rpm; and adjusting a pH of the reaction system to 7.0 by adding a sodium hydroxide solution with a concentration of 1 mol/L dropwise, and adjusting the pH to 10 to 11 by adding a small amount of the sodium hydroxide solution; d. during the process of adjusting the pH, precipitating out a solid slowly; e. filtering out the solid, and washing the solid with a large amount of distilled water until a resulting filtrate changes to neutral from alkaline; f. vacuum-drying the solid at room temperature until there is no residual moisture, wherein the vacuum-drying takes about 7 d; g. adding 600 mg of HA and 300 mL of distilled water to a first beaker to prepare a first solution; adding 12 g of the PEI and 300 ml of distilled water to a second beaker to prepare a second solution; adding the first solution to the second solution, and adjusting a pH of a resulting mixed solution to 6.5 with a 1 M HCl solution to obtain a third solution; dissolving 6.3 mmol of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and 6.3 mmol of 1-hydroxybenzotriazole (HOBt) in a mixed solvent formed by 15 mL of distilled water and 15 mL of dimethyl sulfoxide (DMSO) to obtain a fourth solution to a 1.5 L round bottom flask; and adding the third solution and the fourth solution, stirring a resulting mixed solution at room temperature for 24 h, and adjusting a pH of the resulting mixed solution to 7.0 with a 1M NaOH solution to obtain a reaction solution; h. adding the reaction solution to an activated dialysis bag of 8,000 KD to 14,000 KD, and dialyzing in a 100 mM NaCl solution for 2 d, then in ethanol with a volume fraction of 25% for 1 d, and finally in pure water for 1 d to obtain an HA-PEI liquid; and lyophilizing the HA-PEI liquid for 2 d to obtain a white spongy HA-PEI substance; i. dispersing 10 g of the lyophilized HA-PEI into 50 mL of distilled water, adjusting a pH to 7.1 with a dilute hydrochloric acid solution, and adding distilled water to 100 mL; and j. using a 0.2 μm filter membrane to sterilize a resulting solution to obtain an HA-PEI stock solution with a concentration of 100 mg/mL to produce the artificial exosome complex.
2. The preparation method according to claim 1, wherein the artificial exosome complex is prepared into an aqueous solution with a concentration of 1 mg/mL to 1 g/mL.
3. The preparation method according to claim 1, wherein the HA is disaccharide units composed of D-glucuronic acid and N-acetylglucosamine, and the HA has a molecular weight of 400 to 1,800,000 and is in the form of the HA and sodium hyaluronate (SH).
4. The preparation method according to claim 1, wherein the linear PEI is obtained by heating and hydrolyzing the poly(2-ethyl-2-oxazoline) in a sulfuric acid aqueous solution, and has a molecular weight of 1,000 to 100,000.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
(1)
(2)
(3)
(4)
(5)
(6)
DETAILED DESCRIPTION OF THE EMBODIMENTS
(7) The present disclosure is further explained below in conjunction with specific examples, but the present disclosure is not limited thereto.
(8) In the following examples, unless otherwise specified, the methods used are conventional methods, and all reagents used are commercially-available products.
Example 1. Detection of Skin Irritation of the Artificial Exosome Complex
(9) The detection method is as follows.
(10) 10 volunteers were recruited; a freshly-prepared artificial exosome solution with a concentration of 1 mg/mL was evenly applied at an inner side of the left wrist, and double distilled water (DDW) was applied at an inner side of the right wrist, which used as a control group; and after the application, the changes of the inner side of the wrist that was standing was continuously observed for 20 min and it was recorded whether the volunteer had pain, itch, and other irritating symptoms.
(11) Experimental results were shown in
(12) TABLE-US-00001 TABLE 1 Statistical results of the skin test for the artificial exosome Control (number Exocrinetrater (number of volunteers) of volunteers) Pain Yes 0 0 No 10 10 Itch Yes 0 0 No 10 10 Redness and Yes 0 0 Swelling No 10 10
(13) The experimental conclusion is as follows.
(14) Within the 20 min after the artificial exosome was applied, none of the 10 volunteers experienced the skin irritation symptom of redness and swelling, and none of the 10 volunteers had irritating pain, itch, etc.
Example 2. Detection of Cytotoxicity of the Artificial Exosome
(15) The detection method is as follows.
(16) Human immortal keratinocyte cells (HaCaT cells) were cultivated, digested, and inoculated in a 96-well plate at 5,000 cells/well; the artificial exosome was prepared into artificial exosome solutions with gradient concentrations, the solutions were added to the HaCaT cells, separately, and the cells were further cultivated for 48 h, the morphology of the cells was observed during the 48 h; and finally the MTT method was used to detect the influence of the artificial exosome on cell proliferation.
(17) Experimental results were shown in
(18) The experimental conclusion is as follows.
(19) The artificial exosome did not show obvious cytotoxicity at the cell level, and had no influence on the cell proliferation.
Example 3. Detection of the Delivery Efficiency of the Artificial Exosome for an Active Substance
(20) The detection method is as follows.
(21) The activity of the green fluorescent protein (GFP)-encoding plasmid pCDNA3.1-eGFP that was delivered by the artificial exosome into HaCat cells was detected.
(22) Experimental results were shown in
(23) The experimental conclusion is as follows.
(24) The artificial exosome can well encapsulate an active substance and effectively deliver the active substance into cells.
Example 4. Detection of the Delivery Route of the Artificial Exosome for an Active Substance
(25) The detection method is as follows.
(26) In order to further detect the delivery route of the artificial exosome for an active substance, intracellular organelles such as mitochondrion and lysosome were stained, and at the same time, the artificial exosome was used to deliver the GFP-encoding plasmid pCDNA3.1-eGFP. The delivery area of the active substance was analyzed through the positioning information of fluorescence.
(27) Experimental results were shown in
(28) The experimental conclusion is as follows.
(29) It shows that the artificial exosome can directly deliver the active substance to mitochondria, and it is speculated that, through this delivery route, the active substance can regulate the mitochondrial function, promote the stability of the mitochondrial function, and finally achieve the effect of anti-inflammation and cytothesis.
Example 5. Detection of the Influence of the Artificial Exosome on the Activity of Rat Skin
(30) The detection method is as follows.
(31) Vista rats were used as experimental animals, and sodium carboxymethyl cellulose (CMC-Na) was used to prepare an artificial exosome encapsulating an active substance into an artificial exosome solution with a concentration of 1 mg/mL. The artificial exosome solution was continuously applied on the back of the rats for one week, and the control group was applied with a CMC-Na gel in which the active substance was dissolved. One week later, skin tissue was collected from the back of rats, immunofluorescence assay (IFA) was conducted to detect the expression of the active protein Cytokeratin for epidermal cells, and the functional penetration of the artificial exosome was reflected through the intensity of fluorescence.
(32) Experimental results were shown in
(33) The experimental conclusion is as follows.
(34) Epithelial cells, when in an active state, express the membrane protein. In the IFA, the expression of Cytokeratin can be reflected through the intensity of green fluorescence, thereby reflecting the activity of epidermal cells. It can be seen from the results that the active substance without the artificial exosome cannot effectively stimulate the expression of Cytokeratin, that is, the cell activity cannot be effectively activated; in contrast, the active substance in the artificial exosome stimulates the high expression of Cytokeratin on the skin tissue cells, that is, the artificial exosome activates the activity of epidermal cells and is conducive to the absorption of an active substance by cells. Moreover, the experimental results show that the artificial exosome can effectively activate the activity of deep skin cells. Since the artificial exosome includes HA, it is speculated that cells in the superficial skin layer such as the cuticular layer and stratum lucidum cannot effectively adsorb the artificial exosome as it cannot effectively express an HA receptor, such that the active substance can be delivered deeply to the prickle cell layer and basal cell layer with HA receptors, which makes the skin care more effective.
(35) Although the examples of the present disclosure have been illustrated and described, it should be understood that those of ordinary skill in the art may make various changes, modifications, replacements and variations to the above examples without departing from the principle and spirit of the present disclosure, and the scope of the present disclosure is limited by the appended claims and legal equivalents thereof.