INHIBITORS OF ANTIBIOTIC RESISTANCE MEDIATED BY ARNT

Abstract

The present invention relates to diterpene compounds of general formula (I) capable of contrasting the antibiotic-resistance mediated by the ArnT enzyme, to their use as a medicament, in particular for use as an adjuvant of an antibiotic therapy in the treatment of antibiotic-resistant bacterial infections. The invention relates also to associations of one or more of the compounds of formula (I) with at least another active ingredient, in particular an antibacterial agent and/or an antibiotic, and compositions comprising one or more compounds of formula (I) or the association according to the present invention and at least one pharmaceutically acceptable excipient and/or carrier as well as to products, in particular medical devices, comprising at least a compound, an association or a composition according to the present invention. Moreover, the invention relates to the use of compounds of formula (I) to sensitize a bacterium to an antibacterial agent or an antibiotic, for example, colistin (polymyxin E) or polymyxin B and to an in vivo or in vitro method for sensitizing a bacterium to an antibacterial agent or an antibiotic comprising the exposure of said bacterium to one or more compounds of formula (I) together with colistin.

Claims

1. An adjuvant in antibiotic therapy comprising a compound of formula (I): ##STR00050## wherein R.sub.1 and R.sub.2 are the same or different and independently selected among: hydrogen, C(R.sub.A).sub.3, OR.sub.A, C(═O)R.sub.A, C(═O)OR.sub.A, CH.sub.2OR.sub.A, CH.sub.2OC(═O)R.sub.A, CH.sub.2OC(═O)OR.sub.A, CH.sub.2OC(═O)(CH.sub.2).sub.nC(═O)OR.sub.A with n=0, 1 or 2, CH.sub.2N(R.sub.A).sub.2, C(═O)N(R.sub.A).sub.2, CH.sub.2NHC(═O)R.sub.A and CH.sub.2C(═O)N(R.sub.A).sub.2; where R.sub.A is selected among: hydrogen, alkyl, hydroxyl, monosaccharide, disaccharide and CH.sub.2-formula (I); R.sub.3 is hydrogen, C(R.sub.B).sub.3 or OR.sub.B; where R.sub.B is selected among: hydrogen, alkyl, hydroxyl, and disaccharide; the endocyclic symbol represents a single or double bond and, when it represents a double bond, the exocyclic symbol binding R.sub.4 to the carbocycle represents a single bond; R.sub.4 is hydrogen when the exocyclic symbol binding R.sub.4 to the carbocycle represents a single bond; or R.sub.4 is oxygen or methylene when the exocyclic symbol binding R.sub.4 to the carbocycle represents a double bond; and pharmaceutically acceptable salts.

2. The adjuvant in antibiotic therapy comprising the compound according to claim 1, wherein R.sub.1 is selected among: C(═O)R.sub.A, C(═O)OR.sub.A, CH.sub.2OR.sub.A, CH.sub.2OC(═O)R.sub.A, CH.sub.2OC(═O)(CH.sub.2).sub.nC(═O)OR.sub.A with n=0, 1 or 2, where R.sub.A is selected among: hydrogen, methyl, hydroxyl, monosaccharide and CH.sub.2-formula I; R.sub.2 is methyl; R.sub.3 is selected among: hydrogen, methyl, hydroxyl and disaccharide; the endocyclic symbol represents a single or double bond and, when it represents a double bond, the exocyclic symbol binding R.sub.4 to the carbocycle represents a single bond; R.sub.4 is hydrogen when the exocyclic symbol binding R.sub.4 to the carbocycle represents a single bond; or R.sub.4 is oxygen or methylene when the exocyclic symbol binding R.sub.4 to the carbocycle represents a double bond.

3. The adjuvant in antibiotic therapy comprising the according to claim 2, wherein R.sub.1 is selected among: CH.sub.2OH, C(═O)OH, C(═O)OCH.sub.3, CH.sub.2OC(═O)(CH.sub.2).sub.2C(═O)OH, CH.sub.2OC(═O)C(═O)OH, CH.sub.2OC(═O)CH.sub.2C(═O)OH, C(═O)O-monosaccharide and CH.sub.2OC(═O)(CH.sub.2).sub.nC(═O)O—CH.sub.2-formula I with n=0, 1 or 2.

4. The adjuvant in antibiotic therapy comprising the compound according to claim 1, wherein the compound is selected among: ent-beyer-15-en-18-ol (FDA); ent-beyer-15-en-18-O-oxalic acid (BBN149); ent-beyer-15-en-18-O-malonic acid (FDM); ent-beyer-15-en-18-O-succinic acid (FDS); glucopyranosyl ester of 4-α-13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]-16β-hydroxy-entkaur-16-en-19-oic] acid (SR1); ent-16-bone-beyeran-19-oic acid (SR2); 1,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl ester of ent-beyeran-19-oic acid (SR3); glucopyranosyl β-D ester of ent-beyeran-19-oic acid (SR4); 13-hydroxy-kaur-16-en-19-oic acid (SR5); 4-α-13-[(2-O-β-D-glucopyranosyl-β-D glucopyranosyl) kaur-16-en-19-oic) acid (SR6); methyl ester of ent-16-oxo-beyeran-19-oic acid (SR7); ent-beyeran-19-O-oxalic acid (SR8); ent-beyeran-19-ol (SR9); ent-beyeran-19-oic acid (SR10) and ent-beyeran-18-O-oxalic acid (FDO-H).

5. The adjuvant in antibiotic therapy comprising the compound according to claim 4, wherein the compound is selected among: ent-beyer-15-en-18-O-oxalic acid (BBN149); ent-beyer-15-en-18-O-malonic acid (FDM); ent-beyer-15-en-18-O-succinic acid (FDS); glucopyranosyl β-D ester of ent-beyeran-19-oic acid (SR4); ent-beyeran-19-O-oxalic acid (SR8); ent-beyeran-19-oic acid (SR10) and ent-beyeran-18-O-oxalic acid (FDO-H).

6. A method for treating bacterial infections comprising administering a therapeutically effective amount of the adjuvant in antibiotic therapy comprising the compound of claim 1 to a subject in need thereof.

7. The method of claim 6, wherein the bacterial infections are antibiotic-resistant bacterial infections.

8. A compound of formula (I′): ##STR00051## wherein the compound is selected among: 1,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl ester of ent-beyeran-19-oic acid (SR3); glucopyranosyl β-D ester of ent-beyeran-19-oic acid (SR4); and ent-beyeran-19-ol (SR9), ent-beyer-15-en-18-O-malonic acid (FDM) and ent-beyeran-18-O-oxalic acid (FDO-H).

9. (canceled)

10. An adjuvant in antibiotic therapy comprising the compound of claim 8.

11. A method of treating bacterial infections comprising a therapeutically effective amount of the compound of claim 8 to a subject in need thereof.

12. The method of claim 11, wherein the bacterial infections are antibiotic-resistant bacterial infections.

13. The method according to claim 6, wherein the bacterial infections are caused by a Gram-negative bacterium selected among Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp, Citrobacter freundii, Salmonella typhimurium, Burkholderia cenocepacia, Yersinia pestis, Yersinia enterocolitica, Proteus mirabilis and Salmonella enterica serovar Typhimurium.

14. The method according to claim 7, wherein the antibiotic-resistant bacterial infections are bacterial infections wherein the antibiotic-resistance is mediated by the aminoribosiltransferase (ArnT) enzyme as measured by mass spectrometry.

15. The method according to claim 6, wherein the infection is an acute or chronic pulmonary, extrapulmonary localized or systemic infection.

16. A composition comprising the adjuvant in antibiotic therapy comprising the compound according to claim 1 and at least another active principle.

17. The composition according to claim 16 wherein the active principle is an antibacterial agent and/or an antibiotic.

18. The composition according to claim 16, wherein the weight ratio between compound of formula (I) and antibacterial agent and/or antibiotic is between 1:1-1:20.

19. The composition according to claim 17, wherein the antibiotic belongs to the class of polymyxins and is preferably colistin (polymyxin E) or polymyxin B.

20. The composition according to claim 16, further comprising a pharmaceutically acceptable excipient or carrier.

21. The composition according to claim 20 which is in liquid, solid or semisolid form.

22. A bandage, gauze, patch, cotton wool, spray, prostheses or probes comprising the adjuvant in antibiotic therapy comprising the compound according to claim 1.

23. (canceled)

24. A method for sensitizing a bacterium to an antibacterial agent or an antibiotic comprising exposing the bacterium to the adjuvant in antibiotic therapy comprising the compound of claim 1.

25. The method according to claim 11, wherein the bacterial infections are caused by a Gram-negative bacterium selected among Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp, Citrobacter freundii, Salmonella typhimurium, Burkholderia cenocepacia, Yersinia pestis, Yersinia enterocolitica, Proteus mirabilis and Salmonella enterica serovar Typhimurium.

26. The method according to claim 12, wherein the antibiotic-resistant bacterial infections are bacterial infections wherein the antibiotic-resistance is mediated by the aminoribosiltransferase (ArnT) enzyme as measured by mass spectrometry.

27. The method according to claim 11, wherein the infection is an acute or chronic pulmonary, extrapulmonary localized or systemic infection

28. A composition comprising the compound according to claim 8 and at least another active principle.

29. The composition according to claim 27, further comprising a pharmaceutically acceptable excipient or carrier.

30. The composition according to claim 28, in liquid, solid or semisolid form.

31. A bandage, gauze, patch, cotton wool, spray, prostheses or probes comprising the compound according to claim 8.

32. A method for sensitizing a bacterium to an antibacterial agent or an antibiotic comprising exposing the bacterium to the compound of claim 8.

Description

DETAILED DESCRIPTION OF FIGURES

[0017] FIG. 1. Checkerboard assays to evaluate the synergistic activity between the compounds of interest, here in particular BBN149, FDS, FDM, SR4, SR8 and SR10, and colistin against the colistin-resistant strain P. aeruginosa PA14 col.sup.R 5. The grey boxes highlight the combinations colistin/compound of interest capable of causing a growth inhibition of at least 90%.

[0018] FIG. 2. Graphs showing the adjuvant effect (grey line) of the compounds of interest, here in particular BBN149, FDM, FDS, SR4, SR8, SR10 and FDO-H, in increasing concentration (abscissa axis) on the MIC of colistin (ordinate axis) against the colistin-resistant strain P. aeruginosa PA14 col.sup.R 5. The adjuvant effect was determined by checkerboard assay. As a control, DMSO at equivalent concentrations (0.02-0.64%; black line) was used. The data shown in the graphs are representative of at least three independent experiments.

GLOSSARY

[0019] In the context of the present description, the term “effective amount” means amount of active compound, association or composition comprising the active compounds of the invention, sufficiently high to provide the desired benefits and at the same time low enough not to cause serious side effects.

[0020] In the context of the present description, “about” refers to the experimental error that may occur during conventional measurements. More specifically, when referring to a value it indicates ±5% of the indicated value and when referring to a range ±5% of the extremes thereof.

[0021] In the context of the present description, the terms “synergism”, “synergy”, “synergistic activity”, “activity in synergy with” and the like must be read in their broadest meaning of simultaneous action of two or more compounds which is generally expressed in a positive sense, that is, in the enhancement of the effectiveness of one or both of them. In particular, the above terms also mean “adjuvant”, “adjuvant activity” and “adjuvant activity with”, or “co-adjuvant”, respectively.

[0022] In the context of the present description, with the wording “antibiotic resistance mediated by the ArnT enzyme” is meant the phenomenon for which a bacterium is resistant to an antimicrobial drug, for example colistin, due to the action of the ArnT enzyme, the enzyme responsible for the aminoaribosylation of the lipid A component of the lipopolysaccharide (LPS). The term “mediated by” is therefore interchangeable with the terms initiated by, promoted by, caused by, exacerbated by and the like. The antibiotic resistance mediated by the ArnT enzyme can be easily identified, for example, by mass spectrometry analysis as described in greater detail in the following detailed description.

DETAILED DESCRIPTION OF THE INVENTION

[0023] The authors of the present invention have isolated and selected diterpenes of natural origin and developed synthetic derivatives thereof capable of inhibiting the ArnT, the enzyme responsible for the aminoaribosylation of the lipid A component of the lipopolysaccharide (LPS).

[0024] In detail, a library consisting of over 1000 compounds of natural origin extracted, purified and characterized by different plants of traditional medicine and synthetic derivatives thereof was screened in silico with respect to the crystallographic structure of the ArnT enzyme in complex with the ligand undecaprenyl phosphate to identify new compounds capable of contrasting/inhibiting the activity of the ArnT enzyme and therefore enhancing the effectiveness of antibiotics in the treatment of bacterial infections caused by antibiotic-resistant Gram-negative bacteria.

[0025] By in silico screening, 18 compounds were identified (see, Table 1) which were then tested for their antibacterial activity and for their synergistic or adjuvant activity with different antibiotics, in particular colistin, against an antibiotic-resistant strain, in particular colistin-resistant, of P. aeruginosa grown in the absence or presence of a sub-inhibitory concentration of colistin.

[0026] The results obtained from the microbiological essays indicated the compound ent-beyer-15-en-18-O-oxalic acid (BNN149) and its derivatives as new potential agents capable of increasing the effectiveness of antibiotics in the treatment of antibiotic-resistant bacterial infections and, in particular, resistant to colistin.

[0027] Therefore, the present invention relates to compounds of formula (I):

##STR00002##

wherein
R.sub.1 and R.sub.2 are the same or different and independently selected among: hydrogen, C(R.sub.A).sub.3, OR.sub.A, C(═O)R.sub.A, C(═O)OR.sub.A, CH.sub.2OR.sub.A, CH.sub.2OC(═O)R.sub.A, CH.sub.2OC(═O)OR.sub.A, CH.sub.2OC(═O)(CH.sub.2).sub.nC(═O)OR.sub.A with n=0, 1 or 2, CH.sub.2N(R.sub.A).sub.2, C(═O)N(R.sub.A).sub.2, CH.sub.2NHC(═O)R.sub.A and CH.sub.2C(═O)N(R.sub.A).sub.2; where R.sub.A is selected among: hydrogen, alkyl, hydroxyl, monosaccharide, disaccharide and CH.sub.2-formula (I);
R.sub.3 is hydrogen, C(R.sub.B).sub.3 or OR.sub.B; where R.sub.B is selected among hydrogen, alkyl, hydroxyl and disaccharide;
the endocyclic symbol represents a single or double bond and, when it represents a double bond, the exocyclic symbol binding R.sub.4 to the carbocycle represents a single bond;
R.sub.4 is hydrogen when the exocyclic symbol binding R.sub.4 to the carbocycle represents a single bond; or R.sub.4 is oxygen or methylene when the exocyclic symbol binding R.sub.4 to the carbocycle represents a double bond; and pharmaceutically acceptable salts thereof for the use as an adjuvant in an antibiotic therapy, in particular for the use in the treatment of bacterial infections, even more particularly antibiotic-resistant bacterial infections.

[0028] More in particular, the present invention refers to compounds of formula (I) wherein R.sub.1 is selected among: C(═O)R.sub.A, C(═O)OR.sub.A, CH.sub.2OR.sub.A, CH.sub.2OC(═O)R.sub.A, CH.sub.2OC(═O)(CH.sub.2).sub.nC(═O)OR.sub.A with n=0, 1 or 2, wherein R.sub.A is selected among: hydrogen, methyl, hydroxyl, monosaccharide and CH.sub.2-formula I;

R.sub.2 is methyl:
R.sub.3 is selected among: hydrogen, methyl, hydroxyl and disaccharide;
the endocyclic symbol represents a single or double bond and, when represents a double bond, the exocyclic symbol binding R.sub.4 to the carbocycle represents a single bond;
R.sub.4 is hydrogen when the exocyclic symbol binding R.sub.4 to the carbocycle represents a single bond; or R.sub.4 is oxygen or methylene when the exocyclic bond binding R.sub.4 to the carbocycle represents a double bond; and pharmaceutically acceptable salts thereof for the use as an adjuvant in an antibiotic therapy, particularly for the use in the treatment of bacterial infections, even more particularly antibiotic-resistant bacterial infections.
In one embodiment, R.sub.1 can be selected among: CH.sub.2OH, C(═O)OH, C(═O)OCH.sub.3, CH.sub.2OC(═O)(CH.sub.2).sub.2C(═O)OH, CH.sub.2OC(═O)C(═O)OH, CH.sub.2OC(═O)CH.sub.2C(═O)OH, C(═O)O-monosaccharide and CH.sub.2OC(═O)(CH.sub.2).sub.nC(═O)O—CH.sub.2-formula (I) with n=0, 1 or 2.

[0029] The terms monosaccharide and disaccharide in the context of the present invention are as commonly understood in the art and can thus indicate, respectively, any monosaccharide (for example glucose, fructose, galactose and mannose) and any disaccharide (for example sucrose, maltose, lactose, trehalose, gentiobiose and cellobiose). In one embodiment, the monosaccharide is selected between glucose and o-acetyl-glucose. In an embodiment the disaccharide is selected among sucrose, maltose and lactose.

[0030] In particular, the invention relates to a compound for the use as an adjuvant in an antibiotic therapy, wherein the compound is selected among: ent-beyer-15-en-18-ol (FDA); ent-beyer-15-en-18-O-oxalic acid (BBN149); ent-beyer-15-en-18-O-malonic acid (FDM); ent-beyer-15-en-18-O-succinic acid (FDS); glucopyranosyl ester of 4-α-13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl) oxy]-16β-hydroxy-entkaur-16-en-19-oic] acid (SR1); ent-16-oxo-beyeran-19-oic acid (SR2); 1,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl ester of ent-beyeran-19-oic acid (SR3); glucopyranosyl β-D ester of ent-beyeran-19-oic acid (SR4); 13-hydroxy-kaur-16-en-19-oic acid (SR5); 4-α-13-[(2-O-β-D-glucopyranosyl-β-D glucopyranosyl) kaur-16-en-19-oic) acid (SR6); methyl ester of ent-16-oxo-beyeran-19-oic acid (SR7); ent-beyeran-19-O-oxalic acid (SR8); ent-beyeran-19-ol (SR9); ent-beyeran-19-oic acid (SR10) and ent-beyeran-18-O-oxalic acid (FDO-H).

[0031] Even more in particular, the invention refers to a compound as an adjuvant in an antibiotic therapy, wherein the compound is selected among: ent-beyer-15-en-18-O-oxalic acid (BBN149); ent-beyer-15-en-18-O-malonic acid (FDM); ent-beyer-15-en-18-O-succinic acid (FDS); glucopyranosyl β-D ester of ent-beyeran-19-oic acid (SR4); ent-beyeran-19-O-oxalic acid (SR8); ent-beyeran-19-oic acid (SR10) and ent-beyeran-18-O-oxalic acid (FDO-H).

[0032] The bacterial infections mentioned above can be, for example, bacterial infections caused by Gram-negative bacteria such as Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp, Citrobacter freundii or Acinetobacter baumanni. More in particular, infections caused by Pseudomonas aeruginosa.

[0033] The antibiotic-resistant bacterial infections mentioned above can be, for example, antibiotic-resistant bacterial infections wherein the antibiotic-resistance is mediated by the enzyme transferase ArnT.

[0034] The antibiotic resistance mediated by ArnT, in particular the resistance to colistin mediated (caused, promoted, initiated, exacerbated) by ArnT, can be detected or determined according to any one of the methods known in the art, for example, using the analysis of lipid A by mass spectrometry, as for example described in (Lo Sciuto A, Martorana A M, Fernández-Piñar R, Mancone C, Polissi A, Imperi F. Pseudomonas aeruginosa LptE is crucial for LptD assembly, cell envelope integrity, antibiotic resistance and virulence. Virulence. 2018; 9(1): 1718-1733. doi:10.1080/21505594.2018.1537730).

[0035] By way of example, but in no limitative way, the analysis of mass spectrometry to determine the modification of lipid A with the aminoarabinose, catalysed by ArnT, can be carried out according to the following experimental protocol. For the extraction of lipid A, 2 mL of a bacterial culture in an early stationary phase are centrifuged at 3000×g for 10 minutes and the bacterial sediment resuspended in 400 μL of 70% isobutyric acid and 1 M ammonium hydroxide (in a 5:3 ratio). The samples are incubated for 1 hour at 100° C. and centrifuged at 2000×g for 15 minutes. The supernatants are added to 400 μL of water free of endotoxins, frozen at −80° C. and freeze-dried in a vacuum centrifuge. The resulting sediment is washed with 1 mL of methanol and lipid A is extracted from the sediment using 100 μL of a solution of chloroform, methanol and water (in a 3:1:0.25 ratio). After centrifugation at 2000×g for 15 minutes, 2 μL of supernatant are mixed with 2 μL of norharmane matrix resuspended at 10 mg/ml in a solution of chloroform and methanol (in a 2:1 ratio) and 0.5 μL of this mixture are analysed in a time-of-flight mass spectrometer with laser desorption/ionisation assisted by matrix (Matrix-assisted laser desorption/ionization Time of flight or MALDI-TOF) (5800 MALDI TOF/TOF Analyzer, Sciex). The spectral data can be analyzed with the 4000 series Explorer software version 4.1.0 (Sciex, Ontario, Canada) and used to estimate the lipid A forms based on the structures and molecular weights predicted on the basis of the literature for each bacterium. A reference strain, which does not present aminoarbinose on lipid A, may be included in the analysis as a comparison.

[0036] Therefore, the present invention also relates to compounds according to any one of the embodiment herein described for the use in the treatment of bacterial infections, more particularly of antibiotic-resistant bacterial infections, still more particularly antibiotic-resistant bacterial infections wherein the antibiotic-resistance is mediated by Arnt, wherein the antibiotic resistance mediated by Arnt is determined, for example, by extraction of the lipid A and mass spectrometry.

[0037] The above-mentioned antibiotic-resistant bacterial infections can be, for example, polymyxins-resistant bacterial infections, in particular colistin- or polymyxin B-resistant, wherein the antibiotic-resistance is mediated by the enzyme transferase ArnT.

[0038] The antibiotic resistance mediated by ArnT, in particular the resistance to colistin mediated (caused, promoted, initiated, exacerbated) by ArnT, can be identified or determined according to any of the methods known in the art, for example, using the method of microdilutions (BMD, Broth microdilution) for the determination of the minimum inhibitory concentration (MIC), test approved by the European Committee on Antibiotic Susceptibility Testing (EUCAST) and by the Clinical and Laboratory Standards Institute (CLSI) (see, European Committee on Antimicrobial Susceptibility Testing (EUCAST), Breakpoint Tables for Interpretation of MICs and Zone Diameters, Vol. 8, EUCAST, Va “xjo”, Sweden, 2018; and CLSI, Clinical and Laboratory Standards Institute, M07-A10: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, Vol. 35, CLSI, Wayne, Pa., USA, 10.sup.th edition, 2015). By way of example, MIC assays can be performed in 96-well microtitration plates. In each column of wells are aliquoted 100 μl of MH medium containing decreasing concentrations of colistin (dilutions in reason of 2 from 256 to 0.5 μg/ml) or devoid of colistin, and in each row of wells further 100 μl of MH medium containing 1×10.sup.6 cells/ml of each bacterial strain of interest, so as to reach a final volume of 200 μl, a concentration of bacteria equal to 0.5×10.sup.5 cells/ml and colistin concentrations in the range 0-128 μg/ml. The plates are incubated at 37° C. without stirring for 24 hours, and the MIC is visually assessed as the minimum concentration of colistin capable of causing absence of turbidity in the well.

[0039] Therefore, the present invention also refers to compounds according to any embodiment herein described for the use in the treatment of bacterial infections, in particular antibiotic-resistant bacterial infections, more particularly polymyxin-resistant bacterial infections, even more particularly colistin-resistant, wherein the resistance to colistin is determined by the microdilution method.

[0040] In addition to those mentioned above, infections known in the art for possessing this antibiotic-resistance mechanism, in particular to colistin, are those caused for example by Salmonella typhimurium, Burkholderia cenocepacia, Yersinia pestis and Yersinia enterocolitica, Proteus mirabilis and Salmonella enterica serovar Typhimurium.

[0041] In a preferred embodiment, the bacterial infection may be an acute or chronic pulmonary, extra-pulmonary localized or systemic infection.

[0042] The compounds of the invention can be advantageously associated in a combination product comprising one or more compounds of formula (I) (i.e. an ArnT inhibitor) and at least one other active principle.

[0043] Therefore, the present invention also refers to the association of one or more of the compounds as defined above with at least one other active principle. In one embodiment, said active principle is an antibacterial agent and/or an antibiotic.

[0044] The antibacterial agent and/or the antibiotic can be any antibacterial agent or antibiotic, in particular any antibacterial agent or antibiotic the mechanism of action of which provides for electrostatic interaction with the lipopolysaccharide of the bacterial wall. In one embodiment, the antibiotic can be a peptididic antibiotic. In a preferred embodiment, the antibiotic belongs to the class of polymyxins and is preferably colistin (polymyxin E) or polymyxin B, more preferably colistin. The efficacy of polymyxins, in particular colistin and polymyxin B, can be undermined by bacteria that have the same resistance mechanism, that is the one mediated by ArnT (see Jeannot K, Bolard A, Plésiat P. Resistance to polymyxins in Gram-negative organisms. Int J Antimicrob Agents. 2017; 49(5): 526-535. Doi: 10.1016/j.ijantimicag.2016.11.029).

[0045] Although this is not an essential feature for the purposes of therapeutic efficacy, the inventors of the present invention have also found that, when the weight ratio between compound of formula (I) and antibiotic in the association of the present invention is between 1:1-1:20, the association is able to perform its beneficial effects optimally.

[0046] The compounds and the association of the invention can be included in a pharmaceutical composition.

[0047] Therefore, the present invention also relates to compositions comprising, or consisting of, one or more compounds of the invention and at least one suitable pharmaceutically acceptable excipient or carrier, or to compositions comprising the association of the invention and at least one suitable pharmaceutically acceptable excipient or carrier. In one embodiment, the invention relates to compositions comprising one or more compounds of formula (I), at least one antibacterial agent and/or an antibiotic and at least one suitable pharmaceutically acceptable excipient or carrier.

[0048] Said excipient and/or additive may be selected among those generally known in the art such as, for example: carriers, fillers, humectants, disintegrating agents, binders, retardants, absorption accelerators, wetting agents, surfactants, adsorbents, lubricants, glidants, flavouring agents, sweeteners and/or preservatives.

[0049] The skilled in the art will be able to select appropriate additives/excipients thanks to the general knowledge in the field.

[0050] The compositions according to any of the embodiments provided in the present description, can be formulated in any form, administered by any route of administration and associated with any other component, in a variety of ways.

[0051] In particular, the compositions of the invention can be in liquid, semi-liquid, solid or semi-solid form.

[0052] The compositions are preferably, but not exclusively, administered orally or topically. Suitable liquid forms are, by way of non-limiting example, drops, emulsions, solutions, suspensions (prepared or extemporaneous), syrups and elixirs. The liquid or semi-liquid formulations may be contained in suitable delivery carriers. Suitable solid forms are, by way of non-limiting example, tablets, hard or soft capsules, pills, jellies, lozenges, powders, granulates, sachets and films. The solid dosage forms may also be coated with enteric, gastric or other coatings known in the state of the art. Suitable semi-solid forms are, by way of non-limiting example, ointments, gels, salves, creams and pastes.

[0053] In a preferred embodiment, the composition is formulated in the form of a solution, suspension, cream or ointment.

[0054] The formulations, according to any of the embodiments herein described, can be prepared according to conventional methods known to the person skilled in the art.

[0055] Moreover, the compounds, the associations or the compositions of the invention as defined above may be included in a suitable medical device useful in the treatment of bacterial infections. Non-limiting examples of medical devices suitable for the purposes of the present invention are: bandages, gauzes, patches, cotton wool, spray, prostheses, probes etc.

[0056] Therefore, the present invention also relates to medical devices comprising one or more compounds, the association or composition of the invention, wherein the compounds, the association and composition are as defined above. In particular, the invention relates to bandages, gauzes, patches, cotton wool, sprays, prostheses, probes, preferably bandages, gauzes and patches, comprising one or more compounds, the association or composition of the invention, where the compounds, the association and composition are as defined above.

[0057] The different components or active ingredients of the composition of the invention can be present in variable quantities.

[0058] Furthermore, according to any of the embodiments, the compounds, the association, the compositions and/or the medical devices of the present invention are mainly intended for use by humans, but can also be used on animals.

[0059] The present invention also relates to the use of the compounds, association or compositions as described above, for sensitizing a bacterium, in particular a gram-negative bacterium as defined above, to an antibacterial agent or an antibiotic. In particular, the antibiotic can be an antibiotic belonging to the class of polymyxins such as, for example, colistin (polymyxin E) or polymyxin B.

[0060] The present invention therefore also refers to an in vitro or in vivo method for sensitizing a bacterium to an antibacterial agent or an antibiotic which comprises the exposure of a bacterium to one or more compounds of formula (I) or to the association of the invention.

[0061] As anticipated above, in addition to the compounds of formula (I), the inventors have also isolated novel diterpenes of natural origin and designed and developed several synthetic analogues with ent-beyeranic and ent-kauranic structure of BNN149 so as to enhance the activity and selectivity thereof towards the ArnT enzyme.

[0062] Therefore, the present invention also refers to compounds of formula (I′):

##STR00003##

wherein
R.sub.1 is selected among C(═O)OR.sub.A, CH.sub.2OR.sub.A and CH.sub.2OC(═O)CH.sub.2C(═O)OR.sub.A, where R.sub.A is selected among hydrogen and monosaccharide (where monosaccharide is as defined above);
R.sub.2 and R.sub.3 are methyl; and
the symbol represents a single or double bond; provided that when represents a single bond R.sub.1 is not C(═O)OH and when represents a double bond R.sub.1 is not —CH.sub.2OH; and pharmaceutically acceptable salts thereof.
In an embodiment, the present invention refers to a compound of formula (I′) as defined above wherein
R.sub.1 is selected among C(═O)OR.sub.A, CH.sub.2OR.sub.A and CH.sub.2OC(═O)CH.sub.2C(═O)OR.sub.A, where R.sub.A is selected among hydrogen and monosaccharide (where monosaccharide is as defined above);
R.sub.2 and R.sub.3 are methyl; and
the symbol represents a single or double bond; provided that when represents a single bond R.sub.1 is not C(═O)OH and when represents a double bond R.sub.1 is not —CH.sub.2OH or —C(═O)OH; and pharmaceutically acceptable salts thereof.
In another embodiment, the present invention refers to a compound of formula (I′) as herein defined which does not comprise the following compounds:

##STR00004##

[0063] In an embodiment, the present invention refers to compounds of formula (I′) wherein R.sub.1 is selected among: CH.sub.2OH, C(═O)OH, C(═O)OCH.sub.3, CH.sub.2OC(═O)(CH.sub.2).sub.2C(═O)OH, CH.sub.2OC(═O)C(═O)OH, CH.sub.2OC(═O)CH.sub.2C(═O)OH, C(═O)O-monosaccharide.

[0064] In particular, the present invention refers to a compound selected among: 1,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl ester of ent-beyeran-19-oic acid (SR3); glucopyranosyl β-D ester of ent-beyeran-19-oic acid (SR4); and ent-beyeran-19-ol (SR9), ent-beyer-15-en-18-O-malonic acid (FDM) and ent-beyeran-18-O-oxalic acid (FDO-H).

[0065] The present invention also relates to the compounds of formula (I′) for use as a medicament. In particular, the invention also relates to the compounds of formula (I′) for use as an adjuvant in an antibiotic therapy.

[0066] The present invention also relates to the compounds described above for the use in the treatment of bacterial infections, more particularly of antibiotic-resistant bacterial infections.

[0067] Similarly to what has been described above, the invention also refers to associations, compositions and products which comprise the compounds of formula (I′), that is a subset of the products of general formula (I), their use in sensitizing antibiotic-resistant bacteria and to methods for sensitizing antibiotic-resistant bacteria to antibiotics comprising the exposure of antibiotic-resistant bacteria to said compounds, associations, compositions and/or products.

[0068] In addition, the present invention also refers to processes for the preparation of compounds of general formula (I′) as defined above. More specifically, the invention relates to processes for the preparation of the compounds indicated as SR2, SR3, SR4, SR5, SR6, SR7, SR8, SR9, SR10 and FDO-H, as taught in the following examples and illustrated in the reaction schemes (Schemes 1-11).

[0069] All the compounds of the invention have been tested in vitro on the colistin-resistant reference strain of P. aeruginosa (PA14 col.sup.R 5).

[0070] The analysis of the results obtained by the microbiological essays showed that these compounds have a good ability to reduce the resistance to colistin on the PA14 col.sup.R 5 strain. Moreover, the selected or synthesized compounds showed activity also towards other antibiotic-resistant strains of P. aeruginosa and for antibiotic-resistant clinical strains of other bacteria such as, for example, Klebsiella pneumoniae.

[0071] These results demonstrate that the compounds of the invention are active towards different bacterial species wherein the resistance mechanism is mediated by ArnT, just to name a few, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp, Citrobacter freundii, Salmonella typhimurium, Burkholderia cenocepacia, Yersinia pestis and Yersinia enterocolitica, Proteus mirabilis and Salmonella enterica serovar Typhimurium. As specified above, the antibiotic resistance mediated by ArnT can be identified or determined according to any of the methods known in the art, for example, by using the method of extraction and analysis of lipid A by mass spectrometry as herein described.

[0072] Therefore, as will become clear from the following description and from the annexed examples, said compounds represent a valid alternative for the treatment of antibiotic-resistant bacterial infections, in particular when the resistance is mediated by the ArnT enzyme.

[0073] Other advantages of the compounds of the present invention will be immediately evident to the person skilled in the art on the basis of the previous description and of the examples reported below.

EXAMPLES

[0074] The examples reported below are for illustrative purposes only and are not intended to limit the scope of the present invention. Variations and modifications of any of the embodiments described herein, which are obvious to a person skilled in the art, are included in the scope of the appended claims.

In Silico Screening

[0075] The crystallographic structure of the ArnT protein in complex with the undecaprenyl phosphate ligand identified by the access code PDB 5F15 (Petrou V I, et al., 2016) was used as a rigid receptor in a virtual screening of a library of natural compounds consisting of about 1000 molecules, mainly extracted, isolated and characterized by plants used in traditional medicine of South America. The library is characterized by good chemical diversity and by compounds with drug-like characteristics (Lipinski et al., 1997). The visual inspection of the docking poses and the analysis of the relative scores allowed to select a restricted number of molecules that have ben tested in vitro Table 1.

TABLE-US-00001 TABLE 1 Structure and synergistic or adjuvant activity of the library compounds with a sub-inhibitory concentration of colistin (8 μg/ml)..sup.1 The data were obtained on the P. aeruginosa PA14 CoI.sup.R5 strain, which has a value of MIC and IC.sub.90 of colistin of 64 μg/ml. Without Synergy with colistin colistin (8 μg/ml) Compound Structure IC.sub.50 (μM) IC.sub.50 (μM) IC.sub.90 (μM) BBN36  [00005] >250 >250 >250 BBN53  [00006] >250 >250 >250 BBN79  [00007] >250 250 >250 BBN101 [00008] >250 >250 >250 BBN118 [00009] >250 250 >250 BBN119 [00010] >250 >250 >250 BBN120 [00011] >250 62.5 >250 BBN135 [00012] >250 >250 >250 BBN139 [00013] >250 >250 >250 BBN145 [00014] >250 >250 >250 BBN146 [00015] >250 >250 >250 BBN147 [00016] >250 125 >250 BBN148 [00017] >250 >250 >250 BBN149 [00018] >250 15.625 31.25 BBN151 [00019] >250 >250 >250 BBN152 [00020] >250 >250 >250 BBN153 [00021] >250 15.625 >250 BBN154 [00022] >250 >250 >250 .sup.1The values of IC.sub.50 and IC.sub.90 indicate the minimum concentration of compound able to cause at least 50% and 90% respectively of inhibition of bacterial growth.

Evaluation of the Antibacterial Activity of the Compounds Identified by in Silico Screening

[0076] The compounds identified by in silico screening were tested for their antibacterial activity (in the absence of colistin) and for their synergistic or adjuvant activity with colistin against a colistin-resistant strain of P. aeruginosa grown in the absence or presence of a sub-inhibitory concentration of colistin (8 μg/ml). As reported in Table 1, none of the compounds showed antibacterial activity per se against P. aeruginosa. On the contrary, some compounds were found to inhibit bacterial growth in the presence of colistin (BBN79, BBN118, BBN120, BBN147, BBN149 and BBN153) with IC.sub.50 values (concentration of compound necessary to inhibit bacterial growth of at least 50%) between 16 and 250 μM (Table 1), thus suggesting their synergistic or adjuvant activity with this compound. Among the various compounds with synergistic or adjuvant activity, only the BBN149 compound caused a complete inhibition of the growth of the reference strain, with an IC.sub.50 value (concentration of compound necessary to inhibit bacterial growth of at least 90%) equal to about 30 μM (Table 1).

Isolation of BBN149 Analogues with Diterpene Structure Extracted from Fabiana densa var. ramulosa and Activity Essays

[0077] The BBN149 diterpene was obtained by solid-liquid extraction from the aerial parts of Fabiana densa var. ramulosa, which were collected and identified by the Department of Pharmacological and Toxicological Chemistry, University of Chile.

[0078] The aerial parts (600 g) were left to macerate in acetone for 24 h. Subsequently, the insoluble fraction was separated by filtration and new solvent was added to the solid matrix in order to increase the extraction efficiency. This operation was carried out several consecutive times. The different soluble fractions were collected together and evaporated under vacuum. The filtrate (100 g) was purified by flash chromatography. As a mobile phase, a mixture of hexane:ethyl acetate (EtOAc) was used, starting from 100% of hexane up to a ratio of 97:3 of the eluent system which allowed the isolation of the alcoholic derivative FDA (10%) and a fraction (15 g) composed of a mixture of three products. This latter fraction was subjected to a second chromatographic purification by using a gradient elution. A chloroform (CHCl.sub.3):methanol (CH.sub.3OH) mixture was used as the mobile phase. Using a ratio 98:2 of the eluent system the succinic derivative FDS was isolated with a yield of 20%. The increase in polarity of the mobile phase, obtained using a ratio between the two solvents equal to 97:3, allowed the elution of the malonic derivative FDM with a yield equal to 10%. The further increase in polarity through the use of an eluent mixture CHCl.sub.3:CH.sub.3OH:formic acid in a ratio 97:3:1% allowed the isolation of the oxalic derivative BBN149 with a yield equal to 5%.

[0079] The compounds thus obtained (BBN149, FDA, FDM and FDS) were tested for their antibacterial and synergistic or adjuvant activity with colistin as previously described. According to the results previously obtained for the compound BBN149 (Table 1), none of the compounds showed antibacterial activity per se, while three out of four compounds (BBN149, FDM and FDS) were able to inhibit bacterial growth in the presence of colistin (Table 2). Compared to the reference compound BBN149, however, the FDM and FDS compounds showed a higher IC.sub.50 and an inability to cause complete inhibition of bacterial growth (values of IC.sub.90>250 μM).

TABLE-US-00002 TABLE 2 Structure and synergistic activity with colistin of derivatives of natural origin of the diterpene BBN149 (FDA, FDS and FDM). Data obtained with the P. aeruginosa PA14 CoI.sup.R5 strain, for which the MIC and IC.sub.90 of colistin is equal to 64 μg/ml. Without Synergy with colistin colistin (8 μg/ml) Compound Structure IC.sub.50 (μM) IC.sub.50 (μM) IC.sub.90 (μM) FDA [00023] >250 >250 >250 BBN149 [00024] >250 15.625 31.25 FDS [00025] >250 31.25 >250 FDM [00026] >250 62.5 >250
The structural identity of the isolated compounds was determined by nuclear magnetic resonance spectroscopy (.sup.1H-NMR and .sup.13C-NMR) and mass spectrometry (MS).

Characterization of the Components of the Extract

Ent-beyer-15-en-18-ol (FDA)

[0080] White solid (yield 10%); m.p. 110° C.±0.5° C. [α].sub.D +29.7° (CHCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=5.69 (d, 1H, J=5.7 Hz, H-15); 5.45 (d, 1H, J=5.6 Hz, H-16); 3.41 (d, 1H, J=10.8 Hz, H-18a); 3.11 (d, 1H, J=10.8 Hz, H-18b); 0.99 (s, 3H, CH.sub.3-17); 0.78 (s, 3H, CH.sub.3-19); 0.77 (s, 3H, CH.sub.3-20); .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=136.59, 135.38, 72.48, 61.34, 52.94, 49.19, 49.14, 43.81, 38.90, 37.74, 37.28, 37.16, 35.54, 33.34, 25.11, 20.38, 20.02, 18.12, 17.96, 15.76.

[0081] ESI-MS (positive) m/z: [M+Na].sup.+ calculated for C.sub.20H.sub.32ONa 311.25, found 311.3.

Ent-beyer-15-en-18-O-succinate (FDS)

[0082] Brown solid (yield 20%); m.p. 107.5° C.±0.5° C. [α].sub.D+14.6° (CHCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=5.67 (d, 1H, J=5.6 Hz, H-15); 5.45 (d, 1H, J=5.6 Hz, H-16); 3.88 (d, 1H, J=10.8 Hz, H-18a); 3.68 (d, 1H, J=10.08 Hz, H-18b); 2.67 (m, 4H, HOOC—CH.sub.2CH.sub.2—COOR); 0.99 (s, 3H, CH.sub.3-17); 0.83 (s, 3H, CH.sub.3-19); 0.77 (s, 3H, CH.sub.3-20). .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=177.44, 172.28, 136.60, 135.35, 73.61, 61.24, 52.93, 50.01, 49.05, 43.75, 38.74, 37.29, 37.05, 36.70, 36.01, 33.27, 29.20, 29.06, 25.06, 20.30, 20.20, 17.91, 17.80, 15.63.

[0083] ESI-MS (negative) m/z: [M−H].sup.− calculated for C.sub.24H.sub.35O.sub.4 388.54, found 387.5; [M+Cl].sup.− calculated for C.sub.24H.sub.36O.sub.4Cl 423.40, found 423.20.

Ent-beyer-15-en-18-O-malonate (FDM)

[0084] Green oil (yield 10%); oily. [α].sub.D+25.8° (CHCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=5.67 (d, 1H, J=5.6 Hz H-15); 5.45 (d, 1H, J=5.6 Hz, H-16); 3.98 (d, 1H, J=10.8 Hz, H-18b); 3.75 (d, 1H, J=10.08 Hz, H-18a); 3.44 (s, 2H, HOOC—CH.sub.2—COOR); 0.99 (s, 3H, CH.sub.3-17); 0.85 (s, 3H, CH.sub.3-19); 0.77 (s, 3H, CH.sub.3-20). .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=169.29, 167.81, 136.66, 135.19, 74.66, 61.23, 52.92, 49.88, 49.03, 43.76, 40.53, 38.69, 37.31, 36.97, 36.77, 35.95, 33.26, 25.04, 20.30, 20.24, 17.85, 17.72, 15.62.

[0085] ESI-MS (negative) m/z: [M−H].sup.− calculated for C.sub.23H.sub.33O.sub.4 373.51; found 373.1.

Ent-beyer-15-en-18-O-ossalate (BBN149)

[0086] White solid (yield 5%) m.p. 169.5° C.±0.5° C. [α].sub.D+10° (CHCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=5.67 (d, 1H, J=5.7 Hz, H-15,); 5.46 (d, 1H, J=5.7 Hz, H-16,); 4.10 (d, 1H, J=10.8 Hz, H-18a); 3.90 (d, 1H, J=10.8 Hz, H-18b); 0.99 (s, 3H, CH.sub.3-17); 0.91 (s, 3H, CH.sub.3-19); 0.79 (s, 3H, CH.sub.3-20). .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=158.65, 157.77, 136.74, 135.13, 76.54, 61.17, 52.83, 50.08, 49.01, 43.77, 38.57, 37.36, 36.98, 36.94, 35.92, 33.21, 25.03, 20.35, 20.29, 17.78, 17.62, 15.63.

[0087] ESI-MS (negative) m/z: [M−H].sup.− calculated for C.sub.22H.sub.31O.sub.4 360.49; found 359.4.

Characterization of the Compounds of Natural Origin

[0088] The chemical identity of the compounds examined was verified by Nuclear Magnetic Resonance (NMR). The results obtained were found to be in agreement with those reported in the literature.

[0089] Compound BBN36 (aloin or (10S)-1,8-dihydroxy-3-(hydroxymethyl)-10-[(2S, 3R, 4R, 5S, 6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]-10 H-anthracen-9-one) the NMR analysis is in accordance with what reported in the literature (Jang Hoon K. et al, Journal of Enzyme Inhibition and Medicinal Chemistry, 2017, vol. 32, 78-83).

[0090] BBN53 compound (chlorogenic acid or (1S, 3R, 4R, 5R)-3-[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxy-1,4,5-trihydroxycyclohexane-1-carboxylic acid) NMR analysis is in agreement with what reported in the literature (W. Khoo et al., 2015).

[0091] BBN79 compound (verbascoside or [(2R, 3R, 4R, 5R, 6R)-6-[2-(3,4-dihydroxyphenyl)ethoxy]-5-hydroxy-2-(hydroxymethyl)-4-[(2S, 3R, 4R, 5R, 6S)-3,4,5-trihydroxy-6-methyloxan-2-yl] oxyoxane-3-yl] (E)-3-(3,4-dihydroxyphenyl)prop-2-enoate) the NMR analysis is in agreement with what reported in the literature (Venditti A. et al, 2013).

[0092] Compound BBN101 (floretin or 3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one) the NMR analysis is in agreement with what reported in the literature (Qingwen Hu et al., 2018).

[0093] BBN118 compound (piscidone or 3-[3,4-dihydroxy-6-methoxy-2-(3-methylbut-2-enyl)phenyl]-5,7-dihydroxycromen-4-one) the NMR analysis is in agreement with what reported in literature (Tahara S. et al., 1991).

[0094] Compound BBN119 (Rheediaxantone A or 11,22-dihydroxy-7,7,19,19-tetramethyl-2,8,20-trioxapentacyclo [12.8.0.03,120,04,90,016,21]docosa-1(14), 3(12), 4(9), 5, 10, 15, 17, 21-octaen-13-one) the NMR analysis is in agreement with what reported in the literature (Delle Monache F. et al., 1981).

[0095] Compound BBN120 (rheediaxanthone B or NMR analysis is in accordance with what reported in the literature (Delle Monache F. et aL, 1981).

[0096] Compound BBN135 (harmane or 1-methyl-9H-pyrido[3,4-b]indole) the NMR analysis is in agreement with what reported in the literature (Z. Zhao et al., 2019).

[0097] BBN139 compound (loganine or methyl (1S, 4aS, 6S, 7R, 7aS)-6-hydroxy-7-methyl-1-[(2 S, 3 R, 4 S, 5S, 6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-1, 4a, 5,6,7,7a-haxidrocrociclopenta [c] piran-4-carboxylate) the NMR analysis is in agreement with what reported in the literature (Garaev et al., 2014).

[0098] Compound BBN145 (3-hydroxy-4-methoxy cinnamic acid) the NMR analysis is in agreement with what reported in the literature (Set Byeol K. et al., 2017).

[0099] Compound BBN146 (6-methoxy-7-O-geranyl-coumarin) the NMR analysis is in agreement with what reported in the literature (Fiorito S. et al., 2018).

[0100] Compound BBN147 (6-prenyl-aromadendrine) the NMR analysis is in agreement with what reported in the literature (Toshio F. et al., 1993).

[0101] Compound BBN148 (vismiafenone b or [5,7-dihydroxy-2,2-dimethyl-8-(3-methylbut-2-enyl) cromen-6-yl]-phenylmethanone) the NMR analysis is in agreement with what reported in the literature (Delle Monache et al., 1980).

[0102] Compound BBN149 (ent-beyer-15-en-18-O-oxalic acid) the NMR analysis is in agreement with what reported in the literature (Erazo S. et al., 2002).

[0103] Compound BBN151 (physcione or 1,8-dihydroxy-3-methoxy-6-methyl-anthracene-9,10-dione) the NMR analysis is in agreement with what reported in the literature (Delle Monache F. et al., 1979) Compound BBN152 (rhein or 4,5-dihydroxy-9,10-dioxoanthracene-2-carboxylic acid) the NMR analysis is in agreement with what reported in the literature (Manshuo L. et al., 2017).

[0104] Compound BBN153 (longistiline c or 3-methoxy-4-(3-methylbut-2-enyl)-5-[(E)-2-phenylethylene]phenol) the NMR analysis is in agreement with what reported in the literature (Xing-Yue J. Et al., 2016).

[0105] Compound BBN154 (calcone19 or 4′-O-geranil-calcone) the NMR analysis is in agreement with what reported in the literature (Guglielmi P. et al., 2019).

Design and Synthesis of BB149 Derivatives and Activity Assays

[0106] The results obtained from the microbiological tests suggested the promising role of the diterpene ent-beyerenic scaffold in modulating colistin resistance in colistin-resistant bacterial infections. Given that the BBN149 molecule emerged from the first screening as a promising hit, a first generation of diterpene derivatives was designed and synthesized with the aim of increasing the co-adjuvant ability the action of colistin and delineating the SAR (structure-activity relationship).

[0107] The ent-beyerenic scaffold differs from the ent-kaurenic one in the presence of an exocyclic double bond:

##STR00027##

[0108] Given the possibility of obtaining diterpenes with an ent-beyeranic structure, starting from diterpenes with an ent-kaurenic structure characteristic of the diterpenes of the Stevia rebaudiana var. ramulosa, analogues of the compound BBN149 have been designed wherein the double bond is not present at positions 15 and 16 and the chiral carbon in position 4 has a different stereochemistry (R instead of 3).

[0109] Diterpene derivatives have been tested for their antibacterial and synergistic activity with colistin as previously described. No compound showed antibacterial activity per se, while three compounds (SR4, SR8 and SR10) were able to inhibit bacterial growth in the presence of colistin, with IC.sub.50 values around 15-30 μM (Table 3). No compound was able to completely inhibit bacterial growth (IC.sub.50 values >250 μM).

TABLE-US-00003 TABLE 3 Structure and synergistic or adjuvant activity with colistin of synthetic analogues of diterpene BBN149 (SR1-SR10). Data obtained with the P. aeruginosa PA14 CoI.sup.R5 strain, for which the MIC and IC.sub.90 of colistin is equal to μg/ml. Without Synergy with colistin colistin (8 μg/ml) Compound Structure IC.sub.50 (μM) IC.sub.50 (μM) IC.sub.90 (μM) SR1 [00028] >250 >250 >250 SR2 [00029] >250 >250 >250 SR3 [00030] >250 >250 >250 SR4 [00031] >250 15.625 >250 SR5 [00032] >250 >250 >250 SR6 [00033] >250 >250 >250 SR7 [00034] >250 >250 >250 SR8 [00035] >250 31.25 >250 SR9 [00036] >250 >250 >250  SR10 [00037] >250 31.25 >250 FDO-H [00038] <250 7.812 15,625

Chemical Products, Reagents and Methods of Analysis

[0110] All reagents and solvents are commercially available and have been used without further purification.

[0111] Silica gel (230-400 mesh) was used for purification by flash column chromatography. All reactions were monitored by thin layer chromatography (TLC) and F254 fluorescence silica gel plates (Sigma-Aldrich 99569) were used. The melting points were determined with a Buchi Melting Point B—454 apparatus. The .sup.1H and .sup.13C NMR spectra were recorded with a Bruker 400 Ultra Shield™ instrument (400 MHz for .sup.1H NMR and 100 MHz for .sup.13C NMR), using tetramethyl silane (TMS) as standard. Chemical shifts are reported in parts per million (ppm). The multiplicities were reported as follows: singlet (s), doublet (d), triplet (t) and multiplet (m). Mass spectrometry was performed with the Thermo Finnigan LXQ linear ion trap mass spectrometer, equipped with electrospray ionization (ESI). High resolution mass spectra (HR-MS) were recorded with a Bruker BioApex Fourier transform ion cyclotron resonance (FT-ICR).

Synthetic Procedures

Synthesis of the Compound SR2

[0112] The compound SR1 (SIG MA-ALDRICH 260-975-5) (8.68 mmol, 7.0 g) is treated with hydrobromic acid (48% HBr in water) (21 ml) and the solution, which takes on a brown colour, is left under stirring at room temperature for 16 hours. Subsequently, the precipitate is filtered and solubilized in AcOEt. The organic phase is extracted once with water and once with brine, dehydrated with anhydrous Na.sub.2SO.sub.4 and concentrated at a reduced pressure. The compound SR2 (7.72 mmol, 2.45 g) is obtained by crystallization with CH.sub.3OH. (Lohoelter C. et al., 2013; Avent et al. 1989).

##STR00039##

SR2

[0113] Brown powder (yield 89%); m.p. 230° C.±0.5° C. [α].sub.D −69.3° (EtOH). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=2.64 (dd, 1H, J=18.6 Hz e J=3.7 Hz, 1H, H-15a); 2.07 (d, 1H, J=13.3 Hz, H-3eq); 1.93-1.32 (m, 13H); 1.25 (s, 3H, CH.sub.3-18); 1.23-1.01 (m, 4H); 0.97 (s, 3H, CH.sub.3-17); 0.96-079 (m, 1H, H-1ax); 0.78 (s, 3H, CH.sub.3-20); .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=222.90, 183.50, 57.17, 54.90, 54.44, 48.89, 48.61, 43.81, 41.60, 39.90, 39.63, 38.34, 37.84, 37.46, 29.11, 21.77, 20.49, 19.99, 19.01, 13.48.

[0114] ESI-HRMS (positive) m/z: [M+Na].sup.− calculated for C.sub.20H.sub.30O.sub.3Na 341.20872; found 341.20902.

Synthesis of Compound SR10

[0115] A solution containing SR2 (3.14 mmol, 1.0 g), triethylene glycol (12.5 ml), hydrazine (2.5 ml) and potassium hydroxide (KOH) (22.3 mmol, 1.25 g) is distilled at 180° C., until the removal of a volume of about 1.25 ml. After the distillation is over, the Dean-Stark is removed and the reaction is left under stirring to reflux for 22 hours at 180° C. and, subsequently, for 2 hours at 200° C. Subsequently, the reaction is brought to room temperature and 162.5 ml of distilled water are added. The solution is neutralized with glacial acetic acid (CH.sub.3COOH) 1N. The precipitate is filtered and solubilized in diethyl ether (Et.sub.2O). Finally, this solution is extracted 2 times with water, dehydrated with anhydrous Na.sub.2SO.sub.4 and concentrated under reduced pressure, thus obtaining SR10 (0.911 mmol, 277 mg). (Mosetting E. et al., 1955; Yang et al., 2012).

##STR00040##

SR10

[0116] White powder (yield 29%); melting point: 190° C.±0.5° C. [α].sub.D −42.0° (CHCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=2.13 (d, 1H, J=12 Hz, H-3eq); 2.02-1.24 (m, 15H); 1.22 (s, 3H, CH.sub.3-18); 1.18-0.95 (m, 5H); 0.93 (s, 3H, CH.sub.3-17); 0.89 (m, 1H, H-1ax); 0.84 (s, 3H, CH.sub.3-20). .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=184.04, 60.54, 57.52, 56.33, 45.13, 43.83, 41.54, 40.19, 40.18, 39.45, 38.36, 37.96, 37.69, 33.63, 29.19, 27.26, 21.92, 20.95, 19.09, 14.32.

[0117] ESI-HRMS (negative) m/z: [M−H].sup.− calculated for C.sub.20H.sub.31O.sub.2 303.23295; found 303.23288.

Synthesis of Compound SR3

[0118] To a solution containing SR10 (0.986 mmol, 300 mg) in dichloromethane (CH.sub.2Cl.sub.2) (6.73 ml) and water (1.79 ml), tetrabutylammonium bromide (TBAB) (0.02 mmol, 6.72 mg), potassium carbonate (K.sub.2CO.sub.3) (3.26 mmol, 1450 mg) and acetobromo-α-D-glucose (1.36 mmol, 560 mg) are added. The solution is left under stirring to reflux for 24 hours at a temperature of 50° C. Subsequently, the aqueous phase is extracted with CH.sub.2Cl.sub.2 and the organic phases, in turn, are extracted twice with water, once with brine and finally dehydrated with anhydrous Na.sub.2SO.sub.4. Following evaporation of the solvent under reduced pressure, the compound SR3 (0.572 mmol, 363 mg) was obtained. (Klucik J. et al., 2011; Chaturvedula et al., 2011; Yang et al., 2012).

##STR00041##

SR3

[0119] Brown powder (yield 58%); m.p. 140° C.±0.5° C. [α].sub.D −8.7° (.sup.1H NMR (CD.sub.3OD, 400 MHz): δ (ppm)=5.82 (d, J=8.4 Hz, 1H, H-1′); 5.34 (t, 1H, J=9.4 Hz, H-3′); 5.12-5.05 (m, 2H, H-2′e H-4′); 4.33 (dd, 1H, J=12.1 Hz, J=4.8 Hz, H-6′); 4.02 (dd, 1H, J=12.0 Hz, J=2.4 Hz, H-6″); 4.02-3.98 (m, 1H, H-5′); 2.04 (s, 3H, CH.sub.3CO); 2.02 (s, 6H, 2×CH.sub.3CO); 1.98 (s, 3H, CH.sub.3CO); 1.80-1.25 (m, 16H); 1.17 (s, 3H, CH.sub.3-17); 1.41-0.97 (m, 5H); 0.94 (s, 3H, CH.sub.3-18); 0.77 (s, 3H, CH.sub.3-20). .sup.13C NMR (CD.sub.3OD, 100 MHz): δ (ppm)=177.16, 172.23, 171.55, 171.23, 170.79, 92.50, 74.54, 73.57, 71.77, 69.45, 62.66, 58.53, 58.48, 57.48, 46.16, 45.18, 42.51, 41.12, 41.09, 40.34, 39.29, 39.03, 38.52, 34.82, 29.23, 27.51, 22.96, 21.89, 20.93, 20.52, 14.42.

[0120] ESI-HRMS (positive) m/z: [M+Na].sup.+ calculated for C.sub.34H.sub.50O.sub.11Na 657.32453; found 657.32481.

Synthesis of Compound SR4

[0121] A solution of compound SR3 (0.495 mmol, 314 mg) in CH.sub.3OH: H.sub.2O: hexane (10:2:1) at 10% of Et.sub.3N (7.6 ml) is stirred at room temperature for 48 hours, at the end of which the solution is concentrated under pressure and the residue obtained, the compound SR4 (0.511 mmol, 238 mg), is crystallized with Et.sub.2O at room temperature. (Ouilmi D. et al., 1995; Chaturvedula et al., 2011; Yang et al., 2012).

##STR00042##

SR4

[0122] White powder (quantitative yield); m.p. 160° C.±0.5° C. [α].sub.D −22.7° (MeOH). .sup.1H NMR (CD.sub.3OD, 400 MHz): δ (ppm)=5.42 (d, 1H, J=8.3 Hz, H-1′); 3.83 (dd, 1H, J=12.1 Hz, J=1.6 Hz, H-6′); 3.67 (dd, 1H, J=11.6 Hz, J=4.4 Hz, H-6′); 3.40-3.34 (m, 4H, H-2′, H-3′, H-4′, H-5′); 2.20-2.17 (d, 1H, J=13.2, H-3eq); 2.09-2.03 (m, 1H, H-5eq); 1.91-1.34 (m, 14H); 1.21 (s, 3H, CH.sub.3-17); 1.18-0.97 (m, 5H); 0.94 (s, 3H, CH.sub.3-18); 0.87 (s, 3H, CH.sub.3-20). .sup.13C NMR (CD.sub.3OD, 100 MHz): δ (ppm)=178.27, 95.55, 78.69, 78.66, 74.03, 71.11, 62.42, 59.10, 58.56, 57.57, 46.25, 45.10, 42.75, 41.29, 41.23, 40.32, 39.37, 39.04, 38.53, 34.51, 29.24, 27.60 22.86, 21.93, 20.07, 14.42.

[0123] ESI-HRMS (positive) m/z: [M+Na].sup.+ calculated for C.sub.26H.sub.42O.sub.7Na 489.28227; found 489.28264.

Synthesis of Compound SR9

[0124] To a solution of SR10 (1 mmol, 304 mg) in tetrahydrofuran (THF) (0.0854 n/l, 11.70 ml), lithium tetrahydroaluminate LiAlH.sub.4 (9 mmol, 4.5 ml) is added dropwise. The reaction is left to reflux for about three hours at the end of which the excess of LiAlH.sub.4 is eliminated by adding EtOAc and 20 drops of a saturated solution of Rochelle Salt. The solution is evaporated under pressure to eliminate the excess of THF, extracted with EtOAc and dehydrated with anhydrous Na.sub.2SO.sub.4. The SR9 compound (0.82 mmol, 238 mg) was obtained with a yield of 82%. (Batista et al., 2007; Murillo et al., 2019; Yang et al., 2012).

##STR00043##

SR9

[0125] White powder (yield 82%); m.p. 115° C.±0.5° C.; [α].sub.D −0.7° (CHCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=3.75 (d, 1H, J=8 Hz, H-19b); 3.41 (d, 1H, J=8 Hz, H-19a,); 1.75 (d, 1H, J=12 Hz, H-3eq); 1.72-1.16 (m, 19H); 0.95 (s, 3H, CH.sub.3-17); 0.93 (s, 3H, CH.sub.3-19); 0.89 (s, 3H, H-20). .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=65.82, 57.78, 57.32, 57.14, 45.09, 41.78, 40.10, 39.91, 39.42, 38.67, 37.75, 37.69, 35.69, 33.74, 27.30, 27.21, 20.74, 20.50, 18.25, 15.85.

[0126] ESI-MS (positive) m/z: [M+Na].sup.+ calculated for C.sub.20H.sub.34ONa 313.26; found 313.7.

Synthesis of Compound SR8

[0127] To a solution containing the compound SR9 (0.207 mmol, 60 mg) in Et.sub.2O (0.192 mmol/ml, 1.08 ml) at 0° C., oxalyl chloride (0.414 mmol, 0.207 ml) is added dropwise (ratio between starting substrate and reactive 1:2). The solution is left under stirring for 30 minutes at room temperature. The reaction is then switched off by adding H.sub.2O until there is no more effervescence. The aqueous solution is extracted with Et.sub.2O and the organic phase thus obtained is washed twice with water and once with brine, and dehydrated with Na.sub.2SO.sub.4. The residue is evaporated under pressure and purified through a flash chromatographic column using an eluent mixture CHCl.sub.3: CH.sub.3OH:HCOOH in a ratio 98:2:1%. The SR8 compound (0.190 mmol; 69 mg) was obtained with a yield of 92%. (Zhang X. et al., 2016).

##STR00044##

SR8

[0128] Oil (yield 92%); oily. [α].sub.D −14.3° (CHCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=4.52 (d, 1H, J=10.8 Hz, H-19a); 4.10 (d, 1H, J=10.8 Hz, H-19b); 2.04-1.3 (m, 17H); 1.16-1.03 (m, 5H); 1.00 (s, 3H, CH.sub.3-17); 0.94 (s, 3H, CH.sub.3-19); 0.92 (s, 3H, H-20). .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=158.59, 158.18, 71.10, 57.70, 57.15, 57.01, 45.03, 41.51, 40.03, 39.52, 39.42, 37.67, 37.66, 37.37, 36.06, 33.69, 27.42, 27.26, 20.72, 20.35, 18.05, 15.86.

[0129] ESI-MS (positive) m/z: [M+Na].sup.+ calculated for C.sub.22H.sub.34O.sub.4Na 385.25; found 385.3.

Synthesis of SR6

[0130] The SR1 compound (0.62 mmol, 500 mg) is treated with a 10% solution of potassium hydroxide (KOH) (12.5 ml). The reaction is left under stirring for one hour at a temperature of 100° C. Subsequently, the reaction is cooled to room temperature, neutralized with a solution of CH.sub.3COOH 1N and concentrated under reduced pressure. The SR6 compound (0.589 mmol, 390 mg) was obtained with a yield of 95% by crystallization with CH.sub.3OH. (Wood J R et al., 1955, Chaturvedula et al., 2011).

##STR00045##

SR6

[0131] White powder (yield 95%); m.p. 213° C.±0.5° C. [α].sub.D −32.5° (MeOH). .sup.1H NMR (DMSO-de, 400 MHz): δ (ppm)=5.08 (s, 1H, H-17); 4.74 (s, 1H, H-17b); 4.46 (d, 1H, J=8 Hz, H-1′); 4.35 (d, 1H, J=8 Hz, H-1″); 3.63-2.98 (m, 12H); 2.17-1.22 (m, 17H); 1.07 (s, 3H, H-18); 0.89 (s, 3H, H-20); 0.80-0.70 (m, 2H,); .sup.13C NMR (DMSO-d.sub.6, 100 MHz): δ (ppm)=179.50, 153.41, 104.75, 103.97, 96.36, 84.90, 82.45, 76.98, 76.63, 76.12, 76.02, 75.51, 69.87, 69.75, 60.92, 60.62, 56.56, 53.39, 47.22, 43.87, 43.09, 41.93, 41.27, 41.22, 40.66, 38.37, 36.13, 29.21, 21.95, 19.96, 19.21, 15.35.

[0132] ESI-HRMS (positive) m/z: [M+Na].sup.+ calculated for C.sub.32H.sub.50O.sub.13Na 665.31436; found 665.31488.

Synthesis of Compound SR7

[0133] The SR2 compound (0.943 mmol, 300 mg) is treated, at 0° C., with thionyl chloride (SOCl.sub.2) (13.6 ml) and anhydrous dimethylformamide (DMF) (0.3 ml). The solution is left under stirring for 2 hours at room temperature. Subsequently, the solvent is evaporated under reduced pressure, and anhydrous CH.sub.3OH (54.5 ml) and triethylamine (Et.sub.3N) (12.3 ml) are added at 0° C. The solution is left under stirring for 2 hours at room temperature and, subsequently, the residue obtained by evaporating the solvent is solubilized in CH.sub.2Cl.sub.2. The organic phase is extracted three times with brine and dehydrated with anhydrous Na.sub.2SO.sub.4, leaving it stirring overnight. The SR7 compound (0.943 moles, 313 mg) was obtained with a quantitative yield (Batista et al., 2007; Avent et al., 1989)

##STR00046##

SR7

[0134] Yellow powder (quantitative yield); m.p. 184° C.±0.5° C. [α].sub.D −46.0° (CHCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=3.63 (s, 3H, COOCH.sub.3); 2.63 (dd, 1H, J=18.6 Hz, J=3.8 Hz, H-15ax); 2.18 (d, 1H, J=14.1 Hz, H-3eq); 2.02-1.33 (m, 15H); 1.19 (s, 3H, CH.sub.3-18); 1.14-0.10 (m, 5H); 0.97 (s, 3H, CH.sub.3-17); 0.89 (m, 1H, H-1ax); 0.68 (s, 3H, CH.sub.3-20). .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=177.98, 57.22, 54.89, 54.47, 51.40, 48.86, 48.62, 43.94, 41.64, 39.97, 39.60, 38.10, 37.47, 29.00, 21.86, 20.48, 20.00, 19.10, 13.32.

[0135] ESI-HRMS (positive) m/z: [M+Na].sup.+ calculated for C.sub.21H.sub.32O.sub.3Na 355.22437; found 355.22466.

Synthesis of Compound SR5

[0136] A solution containing SR1 (1.36 mmol, 1.1 g) and sodium periodate (NaIO.sub.4) (7 mmol, 1.5 g) in water (75 ml) is left under stirring at room temperature for 16 hours. Subsequently KOH (134 mmol, 7.5 g) is added to the solution, which is left under stirring to reflux for 1 hour. Thereafter, the solution is neutralized at room temperature with CH.sub.3COOH. The aqueous phase is extracted with Et.sub.2O, while the organic phase is extracted with water and dehydrated with anhydrous Na.sub.2SO.sub.4. The compound SR5 (1.02 mmol, 324.58 mg) is obtained with a yield of 75% by crystallization with CH.sub.3OH. (Batista et al., 2007; Avent et al., 1989).

##STR00047##

SR5

[0137] White powder; 75% yield; melting point: 205° C. ±0.5° C.; [α].sub.D −45.7° (CDCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=4.98 (s, 1H, H-17a); 4.81 (s, 1H, H-17b); 2.22-2.07 (m, 4H); 1.95-1.30 (m, 14H); 1.23 (s, 3H, CH.sub.3-18); 1.10-0.99 (m, 2H); 0.95 (s, 3H, CH.sub.3-20). .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=183.30, 155.85, 103.17, 80.48, 56.99, 53.93, 47.52, 47.03, 43.71, 41.84, 41.35, 40.64, 39.62, 39.45, 37.86, 28.92, 21.92, 20.57, 19.13, 15.55.

[0138] ESI-MS (negative) m/z: [M−H].sup.− calculated for C.sub.20H.sub.30O.sub.3 317.45; found 317.50.

Synthesis of Compound FDA-H

[0139] A solution consisting of FDA (0.329 mmol, 95 mg) and Pd/C (6.55 mg, 10%) in dry EtOH (16.4 ml) is left under stirring, under a hydrogen atmosphere (10 bar), at room temperature for 24 h. The solution is subsequently filtered, and the solvent is evaporated under pressure, obtaining the FDA-H compound (0.327 mmol, 95 mg) with a quantitative yield. (Murillo, J A et al., 2019).

##STR00048##

FDA-H

[0140] White powder (quantitative yield); m.p. 108° C.±0.5° C.; [α].sub.D −4.6° (CHCl.sub.3). .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=3.40 (d, 1H, J=10.8 Hz, H-18a); 3.10 (d, 1H, J=11.2 Hz, H-18b); 2.03 (m, 1H, H-3eq.); 1.61-1.07 (m, 21H); 0.95 (s, 3H, CH.sub.3-17); 0.93 (s, 3H, CH.sub.3-19); 0.75 (s, 3H, CH.sub.3-20); .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=72.45, 57.79, 56.96, 49.67, 45.06, 41.01, 40.14, 39.45, 39.41, 37.77, 37.64, 35.39, 33.90, 29.85, 27.32, 20.63, 20.08, 17.92, 17.90, 15.74.

Synthesis of Compound FDO-H

[0141] To a solution containing the FDA-H compound (0.258 mmol, 75 mg) in Et.sub.2O (0.192 mmol/ml, 1.34 ml) oxalyl chloride (0.516 mmol, 0.26 ml) is added dropwise at 0° C. (ratio between starting substrate and reactive 1:2). The solution is left under stirring to reflux for 30 minutes and at room temperature. The reaction is then quenched by slowly adding distilled H.sub.2O until there is no more effervescence. The aqueous solution is extracted with Et.sub.2O and the organic phase thus obtained is washed twice with water and once with brine, and dehydrated on Na.sub.2SO.sub.4. The residue is evaporated under pressure and purified through a flash chromatographic column using an eluent mixture CHCl.sub.3: CH.sub.3OH: HCOOH in a ratio 98:2:1%. The FDO-H compound (0.196 mmol; 71 mg) was obtained with a yield of 76%. (Zhang, X. et al., 2016).

##STR00049##

FDO-H

[0142] Light yellow oil (yield 76%); [α].sub.D −5° (CHCl.sub.3); .sup.1H NMR (CDCl.sub.3, 400 MHz): δ (ppm)=4.10 (d, 1H, J=10.8 Hz, H-18a); 3.90 (d, 1H, J=10.8 Hz, H-18b); 2.03 (m, 1H, H-3eq.); 0.96 (s, 3H, CH.sub.3-17); 1.70-1.07 (m, 21H); 0.93 (s, 3H, CH.sub.3-19); 0.89 (s, 3H, CH.sub.3-20). .sup.13C NMR (CDCl.sub.3, 100 MHz): δ (ppm)=158.5, 157.80, 57.67, 56.88, 50.62, 44.98, 40.81, 40.04, 39.44, 39.12, 37.75, 37.71, 36.95, 35.83, 33.84, 29.85, 27.27, 20.57, 20.46, 17.62, 17.59, 15.69. ESI-HRMS (negative) m/z: [M−H].sup.− calculated for C.sub.22H.sub.33O.sub.4 361.23843; found 361.23816.

[0143] Biological Activity Assays

[0144] Bacterial Growth Inhibition Assays

[0145] For the evaluation of the antibacterial activity and synergy with colistin of the compounds under examination, a colistin resistant P. aeruginosa strain (PA14 col.sup.R 55) with a minimum inhibitory concentration (MIC) of colistin equal to 64 μg/ml, previously obtained by in vitro growth in the presence of increasing concentrations of colistin (Lo Sciuto e Imperi, 2018). This strain over-expresses the am genes responsible for the modification of lipopolysaccharide by the addition of L-amino-arabinose.

[0146] The strain was grown in Mueller-Hinton (MH) medium for 8 hours, and inoculated at a cell density of ˜10.sup.6/ml in MH medium supplemented or not with colistin at a concentration of 16 μg/ml. One hundred μl of the bacterial cultures were aliquoted in the wells of a 96-well microtitration plate, containing 100 μl of MH medium supplemented with decreasing concentrations of the compound to be tested (500, 250, 125, 62.5, 31.25, 16.625, 8.313 or 4.156 μM) or without compound, so as to bring the bacterial concentration to 5×10.sup.5 cells/ml and that of the compounds to 8 μg/ml for colistin and in the range 0-250 μM for the compounds under examination, in a final volume of 200 μl. The microtitration plates were incubated at 37° C. without stirring for 24 hours. Bacterial growth was assessed by measuring absorbance at 600 nm (A.sub.600) in a microplate reader (Victor 2.sup.V, Perkin-Elmer). Since the compounds are dissolved in DMSO at a concentration of 10 mM, in each assay the bacterial growth is also analyzed in the presence of DMSO at concentrations equivalent to those present in the samples that contain the compounds (0-2.5%) and in the presence or absence of 8 μg/ml colistin. The 50% or 90% inhibitory concentration (IC.sub.50 and IC.sub.90) for each compound was determined as the minimum concentration of the compound capable of causing at least 50% or 90% of bacterial growth inhibition compared to control cultures grown under the same conditions in the presence of an equivalent concentration of DMSO. For each compound three independent experiments were conducted, and the averages of the values obtained in the three experiments are considered to determine the values of IC.sub.50 e IC.sub.90.

Checkerboard Essays

[0147] To further confirm the synergistic activity with colistin of the diterpene compounds under examination, checkerboard essays were carried out, which allow to evaluate the antibacterial activity of the compounds under examination (diterpene derivative and colistin) using different combinations of concentration of the two compounds (FIG. 1). For these essays the most promising compounds were selected based on the previously obtained results (BBN149, FDM, FDS, SR4, SR8 and SR10; Tables 2-3). Again, the greatest synergistic activity was found in the compound BBN149, which was able to reduce the minimum inhibitory concentration (MIC) of colistin for the colistin-resistant strain of P. aeruginosa by 8 times (64 to 8 μg/ml) at concentrations of BBN149≥31 μM, and 4 times (64 to 16 μg/ml) at concentrations of BBN149≥16 μM (FIG. 1). Similarly, the FDO-H compound reduces the MIC of colistin by 8 times at concentrations ≥31 μM while decreasing it by 4 times at concentrations ≥4 μM. Compounds FDS, FDM, SR8 and SR10 showed slightly less activity, managing to cause at most a 4-fold reduction in the MIC of colistin (64 to 16 μg/ml) at minimum concentrations of compound between 16 and 31 μM. Compound SR4 was the least active, being able to reduce the MIC of colistin by 4 times only at a concentration equal to 125 μM (FIG. 1).

[0148] The checkerboard essays were performed in microtiter plates with 96 wells, aliquoting in each column of wells 50 μl of MH medium containing decreasing concentrations of colistin (dilutions in reason of 2 from 384 to 1.5 μg/ml) or without colistin, and in each row of wells further 50 μl of MH medium containing decreasing concentrations of compound of interest (dilutions in reason 2 from 375 to 11.7 μg/ml) or without compound. Finally, 50 μl of the inoculum of the PA14 col.sup.R 5 strain were added to each well at a concentration of ˜1.5×10.sup.6 cells/ml in MH medium, so as to reach a final volume of 150 μl, a bacteria concentration equal to 0.5×10.sup.5 cells/ml, colistin concentrations in the range 0-128 μg/ml and concentrations of compounds of interest in the range 0-125 μM. Plates were incubated at 37° C. without shaking for 24 hours, and bacterial growth was assessed by measuring the A.sub.600 in a microplate reader (Victor 2.sup.V, Perkin-Elmer). Three independent experiments were conducted for each compound, and the averages of the values obtained in the three experiments were considered to determine the IC.sub.90 values.

Minimum Inhibitory Concentration (MIC) Essays: Activity Spectrum and Specificity of the Compound BBN149

[0149] To evaluate the spectrum of activity and the specificity of the compound BBN149, which was found to be the most effective compound in enhancing the activity of colistin (Tables 1-3 and FIG. 1), MIC essays were performed using different strains of P. aeruginosa, both resistant and sensitive to colistin, and colistin-resistant clinical strains of another Gram-negative bacterium, Klebsiella pneumoniae. All the colistin-resistant strains used depend on the aminoarabinosylation of lipid A as a mechanism of resistance to colistin (Lo Sciuto e Imperi, 2018; Esposito et al., 2018). As reported in Table 5, the compound BBN149 was able to reduce the MIC of colistin, by a value between 4 and 16 times, in all the colistin-resistant strains analyzed, both of P. aeruginosa and of K. pneumoniae. Furthermore, the compound showed no relevant activity on colistin-sensitive strains (Table 5). Overall, these data demonstrate that the BBN149 compound is able to specifically interfere with the mechanism of resistance to colistin in various Gram-negative bacteria. MIC essays were performed in 96-well microtitration plates, using previously characterized P. aeruginosa and K. pneumoniae strains (Lo Sciuto e Imperi, 2018; Esposito et al., 2018). In each column of wells were aliquoted 100 μl of MH medium containing decreasing concentrations of colistin (dilutions in reason 2 from 256 to 0.5 μg/ml) or without colistin, and in each row of wells further 100 μl of MH medium containing ˜1×10.sub.6 cells/ml of each bacterial strain of interest and BBN149 or DMSO as a control at a concentration of 120 μM or 1.2% respectively, so as to reach a final volume of 200 μl, an equal concentration of bacteria at 0.5×10.sup.5 cells/ml, colistin concentrations in the range 0-128 μg/ml and concentrations of BBN140 or DMSO equal to 60 μM or 0.6% respectively. The plates were incubated at 37° C. without stirring for 24 hours, and the MIC was visually evaluated as the minimum concentration of colistin capable of causing absence of turbidity in the well. At least three independent experiments were conducted for each strain.

TABLE-US-00004 TABLE 5 MIC of colistin for different strains of P. aeruginosa and K. pneumoniae in the presence of 60 μM BBN149 or 0.6% DMSO as a control. MIC (μg/ml) Specie Strain BBN149 DMSO P. aeruginosa PA14 col.sup.R 5 8 64 PA14 1 0.5 KK1 col.sup.R 1 8 128 KK1 1 0.5 KK27 col.sup.R 6 4 64 KK27 1 0.5 TR1 col.sup.R 6 4 16 TR1 1 0.5 K. pneumoniae KP-Mo-3  16 128 KP-Mo-5  8 128 KP-Mo-6  4 32 KP-Mo-11 8 64 KP-Mo-16 8 64

Cell Viability Test

[0150] The cytotoxic potential of the compounds was evaluated on cell cultures in vitro. For these essays, the most promising compounds were selected based on their antibacterial activity in synergy with colistin. In particular, for the compounds BBN149, FDM, FDS, SR4, SR8 and SR10, cytotoxic activity was determined on human epithelial cells of bronchial origin, 16HBE (Cozens et al, 1994). All compounds were tested in the concentration range of 1.95 μM to 250 μM with an exposure of 18 hours. The results are reported in the table as viability (%) compared to untreated cells (Table 4). Cell viability of control samples treated only with the solvent (DMSO) was equal to 97.11% (±7.65) regardless of the concentration used, in the range between 2.5% and 0.02% (1/2 serial dilutions). Although almost all the compounds show a reduction in cell viability from 15% to 35% at the highest concentrations (250 μM), at concentrations active against P. aeruginosa, cell viability is reduced by a maximum of about 10%. Compounds SR4 and SR10 show a total reduction of cell viability, exclusively at the highest concentration (250 μM). This result can be explained by the reduced solubility of the compound in aqueous media and therefore by the possible precipitation in the cell culture medium.

[0151] To evaluate the cytotoxic potential of the compounds under examination, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) reduction test, commonly used to monitor cell viability was used following the exposure to potentially cytotoxic agents. The essay is based on the reduction of MTT to formazan, a compound that assumes a blue colour which can be evaluated by spectrophotometric reading at a wavelength of 570 nm. For the essay we used the 16HBE cell line. 16HBE cells are derived from human bronchi and their production is reported in Cozens et al (1994). The essay was performed as follows: 3×10.sub.4 cells per well were seeded in 96-well multi-well plates and incubated (37° C., 5% CO.sub.2) to allow adhesion to the bottom of the plate (8-12 hr); the compounds dissolved in DMSO at a concentration of 10 mM were added to a final concentration of 250 μM and diluted 1/2 up to 1.95 μM in the corresponding wells; similarly control samples were treated with 2.5% DMSO (equal to the amount of DMSO present in the samples treated with the compounds at 250 μM) and serially diluted 1/2 up to 0.019%; the cells were then incubated for 18 hours (37° C., 5% CO.sub.2); MTT 0.5 mg/ml was added to each well of the plate and the plate was incubated again for 3 hours (37° C., 5% CO.sub.2); after incubation, the supernatant was removed and the formazan was solubilized in DMSO; the quantity of formazan produced was determined by spectrophotometric reading at a wavelength of 570 nm. Each test was performed in duplicate and three independent replicas were made.

Cell viability was calculated as follows:

C.sub.OD570=OD.sub.570 of sample−OD.sub.570 of wells without cells (white)

Reduction (%)=(C.sub.OD570NT−C.sub.OD570T)/C.sub.OD570NT×100

Viability=100−reduction (%)

where: NT indicates the samples of cells non-threated with the compounds; T the samples of cells treated with the compounds at the different concentrations.

[0152] The mean cell viability values were calculated using the Excel mean and standard deviation functions.

TABLE-US-00005 TABLE 4 Average percentage of cell viability (±standard deviation) μM FDM BBN149 FDS SR4 SR8 SR10 FDO-H DMSO 250 85.01 ± 2.40 65.51 ± 4.05  86.12 ± 3.18  −1.11 ± 2.02 80.16 ± 2.01  1.47 ± 2.29  76.51 ± 12.64  72.15 ± 19.63 125 100.48 ± 12.78 71.99 ± 5.18 105.88 ± 1.31 106.23 ± 5.52 78.02 ± 7.46  92.07 ± 5.47  98.42 ± 16.71 76.74 ± 6.62 62.5 106.63 ± 2.87  76.33 ± 2.65 102.15 ± 3.15  99.27 ± 2.43 85.68 ± 6.99  99.80 ± 4.30  97.14 ± 12.19 85.58 ± 9.56 31.25 113.17 ± 8.95  86.07 ± 5.30 102.95 ± 6.74 101.69 ± 4.39 90.55 ± 4.17  99.52 ± 9.52  93.65 ± 11.27 87.92 ± 5.29 15.63 107.42 ± 0.01  90.80 ± 6.31  99.77 ± 6.58  97.27 ± 5.61 92.64 ± 6.57  97.98 ± 1.66 94.06 ± 3.88 92.29 ± 5.70 7.81 97.93 ± 2.17 86.13 ± 2.37  93.53 ± 2.01  92.44 ± 2.11 90.33 ± 4.26 101.00 ± 7.12 100.25 ± 23.84 90.21 ± 3.93 3.91 96.70 ± 1.60 90.17 ± 7.35  95.34 ± 8.28  92.31 ± 3.26  97.84 ± 10.19 104.95 ± 6.36  94.57 ± 12.67 97.03 ± 9.44 1.95 102.92 ± 7.31  94.15 ± 6.34  98.93 ± 5.38  96.35 ± 9.42 100.17 ± 14.21 105.21 ± 6.73 106.31 ± 17.23 105.83 ± 6.65