ENERGISING AGRICULTURAL FERTILISER COMPOSED OF SUCROSE, HUMIC ACID AND PROTEIN

Abstract

An agricultural energising biostimulating composition made from sucrose, protein/hydrolised protein, and humic acid. The composition is aimed at solving the energy deficit that is affecting the production of fruit and vegetables in the agriculture industry. Plants do not obtain the energy they need for their development, which affects the quality and quantity of the fruit produced.

Claims

1. An agricultural energizing biostimulating aqueous composition comprising 249 to 3,535 grs/liter of carbohydrates, 5 to 100 grs/liter of protein/hydrolyzed protein and 20 to 200 grs/liter humic acids.

2. The composition according to claim 1, wherein the carbohydrates are sucrose, fructose or glucose, or a combination of glucose and fructose.

3. The composition according to claim 1, wherein the protein is of animal or vegetable origin.

4. The composition according to claim 3, wherein the protein of animal origin is may be based on whey, casein, collagen, albumin, or meat protein.

5. The composition according to claim 3, wherein the protein of vegetable origin is from soy, pumpkin, rice, peas, or hemp.

6. The composition according to claim 3, wherein the protein is a hydrolyzed protein.

7. The composition according to claim 1, wherein the composition does not comprise preservatives or additives for conservation.

8. The composition according to claim 2, wherein the composition comprises between 20%-50% of total sugars in the composition, expressed as glucose.

9. The composition according to claim 1, comprising, by weight of non-fat solid matters, 249 to 3,535 grs/liter of carbohydrates, 5 to 100 grs/liter of hydrolyzed proteins and 20 to 200 grs/liter of humic acids.

10. The composition according to claim 9, comprising 2,000 grs/liter of carbohydrates, 10 grs/liter of hydrolyzed proteins and 50 grs/liter humic acids.

11. The composition according to claim 1, comprising a content of macronutrients, of the type N, P.sub.2O.sub.5, K.sub.2O, in an amount which varies between 0% to 1% of the total weight of the composition.

12. The composition according to claim 1, comprising a density between 1.1 and 2.1 g/ml and an acid pH, comprised between 4.5 and 6.

13. A process for obtaining the composition according to claim 1, comprising the following steps: a) preparing an initial saturated solution containing: carbohydrates, protein, humic acid and water, in an autoclave machine with a stirrer and a double jacket; b) raising a temperature of water found in a double layer of the autoclave, using a boiler, to dissolve the saturated solution through indirect heat; c) adding stirring to step b) from a beginning to homogenize the saturated solution from step a); d) continuing for at least 8 hours the process of raising the temperature indirectly with stirring, described in steps b) and c); and e) after completing step d) turning off the boiler and continuously stirring until the mixture reaches room temperature, thus obtaining the agricultural energizing biostimulating aqueous composition.

14. A method of treating energy deficit caused by stress factors in fruit trees and vegetables comprising using the agricultural energizing biostimulating composition according to claim 1.

Description

BRIEF DESCRIPTION OF THE FIGURE

[0028] FIG. 1 represents a diagram of the procedure for obtaining the composition of the present invention.

DETAILED DESCRIPTION OF THE INVENTION

[0029] The composition of the present invention refers itself to a production process based on sucrose, hydrolyzed proteins, and humic acids, which is a direct source of energy for plants, due to its high content of vegetable carbohydrates and proteins, it acts like the glucose that plants should generate in their balanced state, which is their main source of energy for all their metabolic processes, necessary in the different stages of their life.

[0030] Example 1: Composition and preparation procedure of the composition of an agricultural energizing biostimulant based on carbohydrates, hydrolyzed protein and humic acid. Using hydrolyzed collagen as a protein source.

[0031] The manufacture of the agricultural energizing biostimulant composition of the invention is described as follows:

[0032] 200 kg of sugar (1) are incorporated, as well as 5 kg of humic acids (2), and 1 kg of hydrolyzed collagen (3), in 100 liters of distilled water (4) inside the autoclave (5) with stirrer (6) and double jacket (7).

[0033] To homogenize the mixture, the temperature must be raised to 70° C., using a boiler (8) that provides indirect heat, raising the temperature of the water (9) that is between the double layer, further, this process must be complemented with agitation. After 8 hours in the stirring process and at temperatures of 70° C., the mixture achieves a homogeneous consistency. To end this process, only stirring is continued until the product is brought to room temperature.

[0034] After the product is homogeneous and at room temperature, the packaging is carried out, with packaging machines (10) or pumps (11), depending on the format. These can be: 10 l, 20 l, 60 l, 100 l, or 1000 l.

TABLE-US-00001 TABLE 1 Ingredients Range Sucrose (Glucose and Fructose) 249-3,535 g/l Hydrolyzed collagen 5-100 g/l Humic acids 20-200 g/l

Illustration in FIG. 1, Annex at the End of the Description

[0035] Example 2: Composition and preparation procedure of the composition of an agricultural energizing biostimulant based on carbohydrates, hydrolyzed protein and humic acid. Using hydrolyzed whey as protein source.

[0036] The manufacture of the agricultural energizing biostimulant composition is described as follows:

[0037] 200 kg of sugar (1) are incorporated, as well as 5 kg humic acids (2), and 1 kg of hydrolyzed whey (3), in 100 liters of distilled water (4) inside the autoclave (5) with stirrer (6) and double jacket (7).

[0038] To homogenize the mixture, the temperature must be raised to 70° C., using a boiler (8) that provides indirect heat, raising the temperature of the water (9) that is between the double layer, in addition this process must be complemented with agitation.

[0039] After 8 hours in the stirring step and at temperatures of 70° C., the mixture achieved a homogeneous consistency. To end this process, only stirring is continued until the product is brought to room temperature.

[0040] After the product is homogeneous and at room temperature, the packaging is carried out, with packaging machines (10) or pumps (11), depending on the format. These can be: 10 l, 20 l, 60 l, 100 l, or 1000 l.

TABLE-US-00002 TABLE 2 Ingredients Range Sucrose (Glucose and Fructose) 249-3,535 g/l Hydrolyzed whey 5-100 g/l Humic acids 20-200 g/l

Illustration in FIG. 1, Annex at the End of the Description

[0041] Example 3: Composition and preparation procedure of the composition of an agricultural energizing biostimulant based on carbohydrates, protein and humic acid. Using albumin as a protein source.

[0042] The manufacture of the composition, energizing agricultural fertilizer, is described as follows:

[0043] 200 kg of sugar (1) are incorporated, as well as 5 kg humic acids (2), and 1 kg of albumin (3), in 100 liters of distilled water (4) inside the autoclave (5) with stirrer (6) and double jacket (7).

[0044] To homogenize the mixture, the temperature must be raised to 70° C., using a boiler (8) that provides indirect heat by raising the temperature of the water (9) that is between the double layer, in addition this process must be complemented with agitation.

[0045] After 10 hours in the stirring step, since the protein used has less solubility than a hydrolyzed one, and at temperatures of 70° C., the mixture achieves a homogeneous consistency. To end this process, only stirring is continued until the product is brought to room temperature.

[0046] After the product is homogeneous and at room temperature, the packaging is carried out, with packaging machines (10) or pumps (11), depending on the format. These can be: 10 l, 20 l, 60 l, 100 l, or 1000 l.

TABLE-US-00003 TABLE 3 Ingredients Range Sucrose (Glucose and Fructose) 249-3,535 g/l Albumin 5-100 g/l Humic acids 20-200 g/l

Illustration in FIG. 1, Annex at the End of the Description

[0047] Example 4: Use of the composition of the present invention as an agricultural energizing biostimulant, to deliver energy to Cherry Trees and thus achieve a greater and faster shoot growth.

[0048] History: Santana cherry graft holder nursery, located in the Sixth Region of Chile, in the town of Graneros. The nursery presents low development (in the second leaf stage), this could be explained by nematodes, inheritance from previous orchards and possible allelopathies.

[0049] Objective: To deliver the necessary energy to the plants through the developed composition, to activate their own growth and production.

[0050] In this assay, measurements of the shoots were carried out in the control nursery orchard (without treatment), and in the nursery orchard treated with the agricultural energizing biostimulant composition. 20 plants will be used as a representative sample of each group, which their shoot growth will measured in centimeters, then it will be evaluated what percentage of this group is in a certain growth range, indicated in the evaluation table

TABLE-US-00004 Table of growth evaluation of Santana cherry metropolitan region (%) Measurement Measurement Measurement Measurement Measurement Day 0 1 2 3 4 5 Date 20 Sep. 2019 10 Oct. 2019 30 Oct. 2019 10 Nov. 2019 30 Nov. 2019 10 Dec. 2019 Shoot (cm) 10-15 cm 20-30 cm 40-50 cm 60-70 cm 80 > cm Control 100% 10%  0%  0%  0% without treatment Composition 1.sup.st 100% 90% 90% 90% 90% treatment application

[0051] Example 5: Use of the composition of the present invention as an energizing agricultural biostimulant, to deliver energy to the walnut trees and thus achieve an increase in the diameter/size of the fruits.

[0052] History: Orchard with even in its vegetative growth, presents water stress as a result of the situation that exists in this town of Chacabuco in the Metropolitan Region.

Objective

[0053] It is expected to activate the plant from the beginning of root activity and to partially supply the energy deficit caused by abiotic water stress.

[0054] In this test we will demonstrate that, in the application of the program with the composition, the diameter of the walnut fruits increases significantly, in addition to making it remain more active until the fruit is finished.

[0055] Test:

[0056] Significant differences are observed in each of the fruits in the test sample comparing the control with the sample treated with the agricultural energizing biostimulant composition.

[0057] Comparing the control versus the treatment, differences of almost 6 mm in diameter are observed, with a 26.1% increase in sample point A. For point B, 1 mm in diameter and 4.43% increase; Point C, 5.2 mm in diameter and 24.5%; Point D, 7.5 mm in diameter and 34.2%. The initial average diameter for all the control fruits was 16.9 mm, compared to 17 mm for the treatment with the composition, therefore, there is no significant difference in this aspect (see chart 1).

TABLE-US-00005 CHART 1 Table of millimeters (mm) and Percentages (%) Sampling points control table Control A B C D Initial 16.8 15.9 17.8 17.4 Final 38.9 38.7 38.8 39.3 Growth 22.1 22.8 21.1 21.9 Sampling points composition treatment table Treatment A B C D Initial 15.6 19.4 17.8 15.2 Final 43.6 43.2 44.1 44.5 Growth 28 23.8 26.3 29.4 Note: Points A; B; C; D, correspond to measurements averages of the fruits, carried out in four cardinal points of the Orchard.

[0058] Example 6: Use of the composition of the present invention as an agricultural energizing biostimulant to deliver energy to avocados and thereby minimize fruit drop due to abiotic stress effects, improve flowering, fruit set and harvest.

[0059] Physiological Antecedents of Avocados:

[0060] The flowering and fruit setting process in Hass avocados has been widely studied and it has been sought, with highly variable results, to increase production in a stable manner over time.

[0061] Whiley, Lovatt, Wolstenholme, I. Hormaza, Gardiazabal and others have studied in detail the phenology and the process of flowering, fruit set and final fruit set. The conclusion is that it is a complex process that depends on many internal factors of the tree, the climate, the presence of pollen and pollinating agents, among others.

[0062] Specific data: The avocado produces up to a million flowers, from this, if everything works more or less; CCCo of a normal framework, an initial pollination/fruit set of less than 1% can be obtained.

[0063] Finally, after the second summer fall, the final fruit set, if we consider the initial number of flowers, can vary between 0.04-0.02% or less in some cases of stress.

[0064] The initial number of flowers and their quality greatly influence the final number of fruits to be harvested.

[0065] The most common plantation frames in Chile vary between 833 and 416, with many high-density plantations having 1000 to 1333 trees/ha.

[0066] Therefore, depending on the number of trees/ha, the number of fruits per tree can range from 250 to 90, to produce 20 tons/ha, which is considered a good production.

[0067] The potential should be between 300 to 120 depending on the plantation framework to achieve productions between 24 to 28 tons/ha.

[0068] Smaller trees in high density plantation frames (1000 to 1333 trees/ha) should set about 120 fruits/tree, and in medium to low densities, they should produce between 300 to 400 fruits per tree.

[0069] The other detail that needs to be clarified is that not all trees produce well every year (biennial bearing), so the real challenge to obtain good productions is that the productive trees manage to set over 200 and 500 fruits according to their size.

[0070] Having clarified all this, it is clear that the final % of setting, if we start with a universe of 10,000 flowers, should be between 0.05 to 0.02% depending on the size of the trees.

[0071] One of the points most mentioned by researchers is in the reserves of starch and other carbohydrates. The largest reserves are achieved in winter without active shoots and without flowering. These reserves diminish rapidly during flowering/fruit set, fruit development, shoot and root growth, reaching a minimum in late summer/fall during the second flas of vegetative development.

[0072] The next season's flowering depends largely on the tree's starch reserves and other carbohydrates that the tree has at the beginning of the winter. The quality of the flower depends a lot on its size and the carbohydrate reserve it contains.

[0073] There are some works that directly relate the carbohydrate reserves in the flowers at the time of fruit set, and the final production of the fruit.

[0074] All this information motivated us to develop the agricultural biostimulant and energizing to composition, in order to improve the quality of flowering and the final setting of the fruit, i.e., greater stable productions over time. From the study of flowering/fruit set, an application plan is developed, seeking to satisfy the energy needs of the avocado, at its key moments.

[0075] Via Soil, Composition Assay

TABLE-US-00006 Control initial Phenological Dose fruit set Month stage Objective l/ha % assay August/Sept Cauliflower Support flower 5 to 7 0.00% 0.00% bud onset Sept/Oct Flower onset Support 5 to 7 0.05% 0.08% initial fruit development

[0076] Via Leaf: Composition Assay

TABLE-US-00007 Control initial Phenological Dose fruit set Month stage Objective l/ha % assay Sept/Oct Flower onset Support flower 1 to 1.5 0.00% 0.00% onset Sept/Oct 10 days from Support flower/ 1 to 1.5 0.05% 0.08% flower onset fruit set October 10 days from Support flower/ 1 to 1.5 0.03% 0.07% flower onset fruit set Oct/Nov Flower end Support fruit 1 to 1.5 0.025% 0.06% set

[0077] After these applications and depending on the climatic conditions, the load achieved in December and the state of the orchard, it is possible to make a new application plan, aiming to avoid the second fall of fruits, to shift the curve of fruit sizes. and thereby ensuring the production per hectare, and finally ensure a good flowering for the following season.

[0078] Via Soil, Composition Assay

TABLE-US-00008 Control initial Phenological Dose fruit set Month stage Objective l/ha % Assay Dec/January Fruit set/fruit Support fruit 5 to 7 0.025% 0.06% cellular set division February Fruit growth Support fruit 5 to 7 0.02% 0.06% development and set March Fruit growth Fruit growth 5 to 7 0.02% 0.06% and reserves

[0079] We took 200 g fruits as a base. And we think that both treatments contemplate the use of foliar fertilization and growth regulators.

[0080] Base 10,000 initial flowers.