MOLECULAR MARKER, GENE OF MAIZE EAR ROT RESISTANCE AND USE THEREOF

Abstract

The disclosure belongs to the technical field of functional molecular marker, and discloses a molecular marker of maize ear rot resistance, gene and use thereof. In the disclosure a major gene zmSRR1 of maize ear rot resistance is cloned, and the gene's resistance to Fusarium verticillioides is checked by transgenic method. By comparing sequences and a candidate gene association analysis combined with resistance phenotypes, three natural variation sites affecting maize ear rot resistance are confirmed, and are found to be combined into five haplotypes in natural materials. Among which one is a high ear rot susceptible haplotype, so that the steps of detecting variation sites, confirming haplotypes, identifying types of resistance genes in maize, confirming whether disease resistance improvement can be targeted, tracking and monitoring the subsequent breeding are conducted.

Claims

1. A method for developing a functional molecular marker, wherein the method uses a molecular marker of maize ear rot resistance, the molecular marker comprises three variation sites of srr1-1, srr1-2, and srr1-3, respectively for a 34.sup.th base A/G mutation, a 1915.sup.th A/G mutation, and a 2033.sup.th G/T mutation of the zmSRR1 gene sequence.

2.-4. (canceled)

5. A type detection method of a disease resistance gene of maize materials, wherein the method uses a molecular marker of maize ear rot resistance, the molecular marker comprises three variation sites srr1-1, srr1-2, and srr1-3, respectively for a 34.sup.th base A/G mutation, a 1915.sup.th A/G mutation, and a 2033.sup.th G/T mutation of the zmSRR1 gene sequence.

6. A maize molecular breeding method, wherein the method uses a molecular marker of maize ear rot resistance, molecular marker comprises three variation sites of srr1-1, srr1-2, and srr1-3, respectively for a 34.sup.th base A/G mutation, a 1915.sup.th A/G mutation, and a 2033.sup.th G/T mutation of the zmSRR1 gene sequence.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to make technical solutions of embodiments herein more clearly, briefly introduction of drawings used in the embodiments of the present application is shown as follows. Obviously, drawings described below are only some embodiments of the present application. Other drawings can be obtained from the drawings herein without creative work by those skilled in the art.

[0016] FIG. 1 is a flow chart of a method of using a molecular marker of maize ear rot resistance shown in an embodiment of the present disclosure.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0017] In order to make objectives, technical solutions and advantages herein more clearly, combined with the embodiments below, the disclosure is further described in detail. It should be understood that the specific embodiments described here are only used to explain the disclosure, not to limit it.

[0018] In view of the problems in the prior art, the present disclosure provides molecular marker of maize ear rot resistance, gene and use thereof. The present disclosure is described in detail below with reference to the accompanying drawings.

[0019] The molecular markers of maize ear rot resistance of the present disclosure are three variant sites of srr1-1, srr1-2, srr1-3, respectively for the 34.sup.th base A/G mutation, the 1915.sup.th A/G mutation, and the 2033.sup.th G/T mutation in the zmSRR1 gene sequence.

[0020] As shown in FIG. 1, a method of using the molecular marker of maize ear rot resistance provided by an embodiment of the present disclosure comprises:

[0021] S101, cloning a major gene zmSRR1 of maize ear rot resistance;

[0022] S102, checking the gene function by transgenic method;

[0023] S103, comparing sequences by which three natural variation sites affecting the resistance of maize ear rot in gene zmSRR1 are found;

[0024] S104, detecting variation sites and identifying types of resistance genes in maize;

[0025] S105, confirming whether disease resistance improvement can be targeted by association the marker results and phenotype of populations;

[0026] S106, tracking and monitoring the subsequent breeding.

[0027] A major gene zmSRR1 of maize ear rot resistance is cloned in present disclosure (also known as GRMZM2G009818, Zm00001d027645, LOC103631713, MDIS1-interacting receptor like kinase 1), derived from Ming Ju #, Zijian Zhou #, Cong Mu, Xuecai Zhang, Jingyang Gao, Yakun Liang, JiafaChen, Yabin Wu, Xiaopeng Li, Shiwei Wang, Jingjing Wen, Luming Yang*, Jianyu Wu*, Dissecting the genetic architecture of Fusarium verticillioides seed rotresistance in maize by combining QTL mapping and genome-wide associationanalysis, Scientific Reports, 2017, 7(1).

TABLE-US-00001 TABLE 1 Statistical analysis of 3 functional markers and their haplotypes on the resistance phenotype of maize ear rot statistics of incidence grade of ear rot mean and significant phenotypic number of of contribution marker genotype materials difference significant rate srrl-1 A 39 2.87A 4.68E−05 12.0% G 93 2.35B srrl-2 A 76 2.70A 9.61E−04 7.5% G 66 2.32B srrl-3 G 85 2.69A 6.25E−04 8.0% T 57 2.29B haplotype A + 29 3.03a 3.89E−05 18.1% A + G A + 10 2.40b G + T G + 41 2.40b A + G G + 9 2.55b G + G G + 43 2.27b G + T

[0028] Through detection of markers and identification of disease resistance in 142 maize inbred lines, three functional markers are found to be combined into five haplotypes, and contribute to 18.1% of the phenotypic variation, wherein, A+A+G is a high ear rot susceptible haplotype.

TABLE-US-00002 TABLE 2 Numbers of materials and proportions of different haplotypes of SRR1 gene in different maize breeding kin materials detected based on 3 functional markers Total (numbers kin G + G + G G + G + T A + G + T G + A + G A + A + G of materials) CML 3(4.2%) 28(38.9%) .sup. 10(13.9%) 24(33.3%) 7(9.7%) 72 Reid 0(0%).sup.  10(76.9%) 0(0%)  2(15.4%) 1(7.7%) 13 Lan 1(20%)  3(60%)  0(0%) 1(20%)  0(0%).sup.  5 P 1(6.3%)  2(12.5%) 0(0%) 11(68.8%)  2(12.5%) 16 TSPT  3(13.6%) 1(4.5%) 0(0%) 0(0%).sup.  18(81.8%) 22

[0029] Through marker detections of maize inbred lines with different breeding kin, the high ear rot susceptible haplotypes were found concentrated in a kin material with Tangsipingtou (TSPT). Tangsipingtou kin inbred line is the main male parent type of maize planting area in Huang-Huai-Hai, China, with excellent traits in breeding such as early maturity and pollen dispersal, but poor resistance to ear rot. Marker detections show that the proportion of SRR1 gene with susceptible genotypes in Tangsipingtou kin materials is up to 81.8%. Therefore, the molecular markers of ear rot resistance can effectively improve the resistance of most Tangsipingtou kin inbred lines to ear rot and promote the breeding of disease-resistant maize varieties.

[0030] The above are only specific embodiments of the present disclosure, and the protection scope of the disclosure is not limited to this. Within the technical scope disclosed herein, any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present disclosure shall be covered by the claimed scope of the present disclosure.