Liquid invisible mask with broad-spectrum bacteriostasis and preparation method and using method thereof

Abstract

A liquid invisible mask, and a preparation method and a using method thereof are provided by the present disclosure, and the present disclosure relates to the technical field of isolated bacteriostatic protective materials. The liquid invisible mask which is a film covering a surface of a nasal cavity, an oral cavity or a throat mucosa, wherein: the film comprises a garlic extract with garlicin content more than 45%, sesame essential oil and cream; and a mass ratio of the garlic extract, the sesame essential oil and the cream is (70-85): (10-20): (5-10). The present disclosure takes the garlic extract with high content of garlicin as the active component, ensures the basic efficacy of sterilization and toxicity elimination, and is supplemented with sesame essential oil as the film-forming carrier material and cream as the skeleton material.

Claims

1. A liquid clear mask, which is a film covering a surface of a nasal cavity, an oral cavity or a throat mucosa, wherein: the film comprises a garlic extract with garlicin content more than 45%, sesame essential oil and cream; and a mass ratio of the garlic extract, the sesame essential oil and the cream is (70-85):(10-20):(5-10).

2. The liquid clear mask according to claim 1, wherein: content of the garlic extract is 70% to 85% of a total mass of the liquid invisible mask; content of the sesame essential oil is 10% to 20% of the total mass of the liquid invisible mask, and content of the cream is 5% to 10% of the total mass of the liquid invisible mask.

3. The liquid clear mask according to claim 2, wherein: a mass ratio of the garlic extract to the sesame essential oil is (80-85):(10-15).

4. The liquid clear mask according to claim 1, wherein: a mass ratio of the sesame essential oil to the cream is (10-15):(5-10).

5. The liquid clear mask according to claim 1, wherein: the mass ratio of the garlic extract, the sesame essential oil and the cream is (80-85):(10-15):(5-10).

6. The liquid clear mask according to claim 5, wherein: the mass ratio of the garlic extract, the sesame essential oil and the cream is (80-85):(10-15):(5-7).

7. The liquid clear mask according to claim 1, wherein: the garlic extract is obtained by following steps: mixing garlic and extract solution in a weight ratio of 1:(2-5), extracting in a high-pressure water-oil mixed phase, and extracting for 5 h to 12 h under an action of an extraction pressure difference formed by an operation of a high-pressure rotor pump; separating an extracted material and solution, standing the extract solution, layering water-oil, and an oil layer is garlic oil; wherein, drying and crushing the garlic to 60 meshes to 80 meshes; preparing the extract solution in a weight ratio of water:edible refined rapeseed oil as 1:(1-5); a vacuum degree generated by the operation of the high-pressure rotor pump is 0.02 MPa to 0.08 MPa, and a discharge pressure is 0.1 MPa to 0.6 MPa.

Description

BRIEF DESCRIPTION OF DRAWINGS

(1) FIG. 1 is a schematic diagram of structure and functional principle of the liquid invisible mask in the embodiment 1 of the present disclosure;

(2) FIG. 2 is a use effect drawing of the liquid invisible mask in the embodiment 1 of the present disclosure in highly polluting environments;

(3) FIG. 3 is a use effect drawing of the liquid invisible mask in the embodiment 1 of the present disclosure in a normal environment.

DETAILED DESCRIPTION OF THE EMBODIMENTS

(4) The following embodiments are intended to illustrate the present disclosure, but are not intended to limit the scope of the present disclosure.

(5) In the following embodiment, the garlic extract prepared by the following method:

(6) selecting fresh purple garlic without pests and mildew in Longhui and Dongkou of selenium-rich soil area of Xuefeng Mountain, removing garlic peel, washing garlic with water and remove impurities such as sediment;

(7) smashing the washed dry garlic, and using the electric hot air tunnel dryer for baking at the temperature of 60° C. for 12 h; and making mechanical crushing to 80 mesh;

(8) putting the comminuted material into a stirring rotating leaf (36 r/min) leaching tank, adding the extract solution with the weight ratio of water to edible refined rapeseed oil as 1:5, and the weight ratio of garlic to the extract solution as 1:2; extracting for 8 h under an action of an extraction pressure difference formed by the high vacuum degree (0.02 MPa˜0.08 MPa) and discharge pressure (0.1 MPa˜0.6 MPa) produced continuously in an operation of the high-pressure rotor pump;

(9) pressing the extracted material with a press to basically separate the product liquid; applying the residue to the subsequent processing of flavor sauce; leaving the separated extract liquid in a standing tank for 2 h to separate water and oil; testing the oil layer and preparing it into garlicin oil finished product with specific gravity 1.094 G/l˜1.098 G/l, and refractive index (20° C.) 1.550118 4.5502.

(10) Embodiment 1: a preparation method for the liquid invisible mask

(11) The formula is as follows: according to the quality percentage, 85% garlic extract, 10% sesame essential oil and 5% cream.

(12) Specific Preparation Steps:

(13) 1) refining the sesame essential oil in a thermostatic electric interlayer pot with stirring, a temperature 120° C., a time 20 min, then adding the cream, stirring for 10 min, and cooling for use;

(14) 2) adding the garlic extract in the 1), then emulsifying and homogenizing, and mixing evenly to form a stable liquid state.

(15) Wherein, the pipeline high-shear homogenizing emulsifier is used for emulsifying and homogenizing, and the specific operating conditions are: speed 1450-1500R/min; intermittent operation: starting for 3 min, stopping for 2 min, continuing three operation units; and handling 500 L to 1000 L per hour.

(16) FIG. 1 is a schematic diagram of structure and functional principle of the liquid invisible mask in the embodiment 1 of the present disclosure;

(17) FIG. 2 is a use effect drawing of the liquid invisible mask in the embodiment 1 of the present disclosure in highly polluting environments; the highly polluting environment usually refers to a hospital or a crowded place. In a highly polluting environment, a layer of medical mask can be externally applied, and a layer of liquid invisible mask of the disclosure can be used inside, so as to play a double protection and be safer.

(18) FIG. 3 is a use effect drawing of the liquid invisible mask in the embodiment 1 of the present disclosure in a normal environment. In the normal environment, the liquid invisible mask can be used to replace the traditional external mask, and the protective film can be formed in the nasal cavity, the oral cavity, the throat and other parts of the user through the spraying equipment, so that most of the germs and dust can be isolated, and the susceptible population can be protected.

(19) Embodiment 2: the preparation method for the liquid invisible mask

(20) The preparation method is the same as Embodiment 1, and the difference is that the formula is as follows: according to the quality percentage, 70% garlic extract, 20% sesame essential oil and 10% cream.

(21) Embodiment 3: the preparation method for the liquid invisible mask

(22) The preparation method is the same as Embodiment 1, and the difference is that the formula is as follows: according to the quality percentage, 80% garlic extract, 15% sesame essential oil and 5% cream.

(23) Effect Investigation

(24) The protective effect, safety, irritation and other aspects of the liquid invisible mask obtained in Embodiment 1 and Embodiment 2 are tested.

(25) 1. Anti-Bacterial and Virus Inactivation

(26) The test basis is GB15979-2002.

(27) TABLE-US-00001 TABLE 1 Bacteriostatic Effect of Sample Stock Solution of Embodiment 1 on Staphylococcus aureus Control group Number of colonies Bacteriostatic Test No. (CFU/mL) rate for 5 min 1 1.87 × 10.sup.4 86.31 2 2.81 × 10.sup.4 85.69 3 3.29 × 10.sup.4 85.07 Average 2.66 × 10.sup.4 85.69 Note: sterile growth of negative control

(28) TABLE-US-00002 TABLE 2 Bacteriostatic Effect of Sample Stock Solution of Embodiment 1 on Escherichia coli Control group Number of colonies Bacteriostatic Test No. (CFU/mL) rate for 5 min 1 4.25 × 10.sup.4 91.20 2 2.40 × 10.sup.4 93.12 3 3.17 × 10.sup.4 91.82 Average 3.27 × 10.sup.4 92.05 Note: sterile growth of negative control

(29) TABLE-US-00003 TABLE 3 Bacteriostatic Effect of Sample Stock Solution of Embodiment 1 on Candida albicans Control group Number of colonies Bacteriostatic Test No. (CFU/mL) rate for 5 min 1 1.32 × 10.sup.4 66.06 2 2.12 × 10.sup.4 64.15 3 3.13 × 10.sup.4 63.44 Average 2.25 × 10.sup.4 64.55 Note: sterile growth of negative control

(30) TABLE-US-00004 TABLE 4 Virus Inactivation Rate Test of Sample Stock Solution of Embodiment 1 in 5 Min Average virus titer total Average virus Laboratory Action logarithm Average virus number of inactivation mortality virus and concentration lgTCIDso/ titer logarithm viruses logarithm rate host and time Group mL lgTCIDso/mL TCID50/mL (KL) (%) Influenza A Stock Control 6.12 5.94 9.15 × 10.sup.5 1.04 90.05 virus Solution group 1 H1N1 Control 5.90 (A/PR/8/34) group 2 Host name: Control 5.80 MDCK cell group 3 Group 1 4.80 4.9 9.10 × 10.sup.4 Group 2 5.20 Group 3 4.71

(31) TABLE-US-00005 TABLE 5 Test Effects of Samples Obtained in Embodiment 1 to Embodiment 2 and Comparative Embodiment Antibacterial effect 5 min virus (5 min) % extinguishing Film- Escherichia Staphylococcus Candida rate % forming coli aureus albicans H1N1 Stability speed Embodiment 92.05 85.69 64.55 90.05 Hold for Very fast 1 9 h to 12 h Embodiment 85.05 76.03 54.55 80.16 Hold for Relatively 2 6 h to 9 faster h Comparative 68.35 62.25 48.36 60.05 Hold for General Embodiment 5 min 1 to10 min Easily absorbed by skin and mucosa Comparative 72.34 65.38 46.72 73.38 Hold for General Embodiment 10 min 2 to 15 min

(32) Comparative Embodiment 1:

(33) the preparation method is the same as Embodiment 1, and the difference is that the formula is as follows: according to the quality percentage, 55% garlic extract, 55% sesame essential oil and 15% cream.

(34) Comparative Embodiment 2:

(35) the preparation method is the same as Embodiment 1, and the difference is that the sesame essential oil is replaced with sweet orange oil, and the cream is replaced with cod liver oil.

(36) The test effect of the sample obtained in Embodiment 3 is comparable to that of Embodiment 1 and Embodiment 2.

(37) 2. Mucosa Safety

(38) According to the classification standard of eye irritation reaction in the Technical Specification for Disinfection (2002 edition), the product obtained in Embodiment 1 to Embodiment 3 is non-irritant in the damage type of New Zealand rabbit eyes.

(39) 3. Skin Irritation

(40) According to the Evaluation Standard for Skin Irritation Intensity of the Technical Specification for Disinfection (2002 Edition), the product obtained in Embodiment 1 to Embodiment 3 is non-irritative to the multiple intact skin of New Zealand rabbits.

(41) 4. Stability and Long-Term Antibacterial Action

(42) TABLE-US-00006 TABLE 6 Stability and long-term antibacterial action of Embodiment 1 Antibacterial Antibacterial effect types 5 min 10 min 15 min 20 min 8 h to 12 h Escherichia coli 92.05% 99.36% 99.94% 99.99% 99.99% Staphylococcus 85.69% 97.95% 99.70% 99.96% 99.99% aureus Candida albicans 64.55% 87.43% 95.54% 99.01% 99.99% Influenza A virus 90.05% 99.00% 99.90% 99.99% 99.99% H1N1

(43) It can be seen from Table 6 that the long-acting bacteriostatic effect and stability of Embodiment 1 are remarkable, and the bacteriostatic and antiviral effects can reach 99.9% after 20 min of application; the liquid invisible mask formed in Embodiment 1 still has good antibacterial effect after 8 h to 12 h of application, which shows that the stability and long-acting bacteriostatic effect are very good. Meanwhile, the stability and long-term bacteriostatic effect of Embodiment 2 and Embodiment 3 are similar to those of Embodiment 1.

(44) 5. Garlicin Content

(45) After testing, the content of the garlicin active ingredient in Embodiment 1 to Embodiment 3 is more than 45%, and the content of garlicin in the product of Embodiment 1 is 47.92%, which is 252 times of the content 0.19 of garlicin active ingredient in garlic.

(46) 6. Determination of Heavy Metals (Lead, Mercury and Arsenic)

(47) Lead, mercury and arsenic are not detected in the product stock solution obtained in Embodiment 1 to Embodiment 3.

(48) 7. Microbiological Indicators

(49) The total bacterial colony count and total fungal colony count of the product stock solution obtained in Embodiment 1 to Embodiment 3 are all less than 1 CFU/mL, and Escherichia coli group, Staphylococcus aureus, Pseudomonas aeruginosa and hemolytic streptococcus are not detected and meet the requirements of GB 15979-2002 Hygienic Standard for Disposable Sanitary Articles.

(50) Although the present disclosure has been described in detail above with general description and specific embodiments, it will be apparent to those skilled in the art that some modifications or improvements may be made on the basis of the present disclosure. Therefore, these modifications or improvements made without departing from the spirit of the disclosure are within the scope of the disclosure.