Obtaining improved water fortifier from brewer's yeast water

Abstract

A fortifier for growth and flowering in horticulture, trees and ornamental plants, aromatic plants and fruit trees, from wastewater from brewer's yeast with a very low protein concentration. The method including the step of: filtered by 80 μm; magnetic treatment of the liquid; addition of 0.1 to 20 g/l phosphate and 10 to 20 g/l glycose. Fermentation is carried out keeping the free amino acid content greater than 0.1%, the content of Mg.sup.+2 greater than 0.1%, total nitrogen, phosphate, and potassium below 3.0%, the content of protein from 0.5% to 1.0% and EDTA from 0.02% to 0.2%, all by dry matter weight; addition of microbes from the environment; at temperatures of 15° C. to 40° C. with gentle agitation, for 24 hours to 30 days; subsequent filtering and pH adjustment. It has been found to be effective in various crops.

Claims

1. A method for obtaining an improved fortifier from wastewater yeast with low protein concentration comprising: obtaining a sample of wastewater from a brewer's yeast water; filtering the wastewater through an 80 μm pore filter; magnetically treating a liquid portion of the filtering step to obtain a magnetically treated product; adding to the magnetically treated product a mixture containing a shredded and homogenized fish waste in a 0.9% weight saline solution (NaCl); adjusting the pH to 5 of the product of the previous step with acetic acid to form a slurry; adding to the slurry amino acids and a magnesium salt maintaining a free amino acids content greater than 0.1% weight and at least 0.1% weight of a magnesium cation (Mg+.sup.2); keeping in the slurry concentrations of nitrogen, phosphate, and potassium to less than 3% of the total weight of the slurry; fermenting the slurry at temperatures of between 24° C. and 26° C. and subjecting the slurry to a smooth, and continuous motion to form a fermenting product; incubating the fermenting product for 20 to 40 days to obtain a incubating product; filtering the incubating product through an 80 μm pore filter to obtain a filtered product; and adjusting the pH of the filtered product between 4.5 and 7.0.

2. The method according to claim 1, wherein the step of magnetically treating is performed in a device equipped with quartz crystals with gold inlays at a temperature between 15° and 40° C., and a liquid flow passage of between 10 and 100 l/min.

Description

BRIEF DESCRIPTION OF THE FIGURES

(1) FIGS. 1a and 1b display examples of conventional production cuttings with addition of the fortifier respectively, grown in parallel for 5 weeks;

(2) FIG. 2a displays the treated cuttings with addition of the fortifier that are on the front tray and in FIG. 2b are on the right tray;

(3) FIG. 3a displays the cuttings groups treated with addition of fortifier, and FIG. 3b displays those that are untreated;

(4) FIG. 4 displays the geranium cuttings treated with the addition of fortifier;

(5) FIG. 5 displays the differences in root growth when applying fortifier in growing Dimorphotheca plants;

(6) FIG. 6 displays the general appearance of Dimorphotheca plants treated before flowering;

(7) FIG. 7 displays the fortifying action in growing Dimorphotheca plants; and

(8) FIG. 8 displays the differences in vigor and flowering of treated Dimorphotheca plants.

DETAILED DESCRIPTION OF THE INVENTION

(9) Obtaining the Fortifier According to the First Embodiment

(10) 900 liters of liquid are placed in a container with a capacity of 1000 liters. It is left to settle for 48 hours. The liquid is filtered through a filter with a pore size of 80 μm and passed through a magnetic treatment kit. 50 liters of 10% concentrated amino acids are added for a total concentration of 1% amino acids in the solution. 25 liters of glucose solution of a 0.4 kg/l concentration and 25 liters of a slurry of 10.sup.8 microbes/ml are added. It is fermented in a room at a controlled temperature of 30° C. for 45 days. At the end of fermentation pH control is performed and it is again passed through a filter with a pore size of 80 μm. The product is defined as finished to be bottled.

(11) The following studies were undertaken with the final product resulting from this same fermentation, with the results below.

(12) Aromatic Plant—Mint

(13) Study carried out in an ecological nursery of aromatic plants with the purpose of determining the final result in the mint cuttings after the application of the fortifier directly to the mother plants.

(14) Comparison of the growth of cuttings from plants treated with the addition of fortifier and plants that have not been. 20 mother plants of this variety were selected, they were watered and conventional fertilizer solution plus fortifier solution were applied. After 1 month the cuttings protruding therefrom began to be monitored and were compared with the nursery's conventional mint production.

(15) The cuttings from the plants treated with the addition of fortifier displayed the following characteristics:

(16) Larger size of the cuttings and faster growth (5 days of nursery were gained).

(17) Greater quantity and size of the roots.

(18) More intense color

(19) Higher survival rate of the cuttings.

(20) Increase of more than 30% of the plant's biological mass.

(21) In FIG. 1a (conventional production) and FIG. 1b (with addition of fortifier) the example of the different cuttings obtained grown in parallel for 5 weeks is displayed. It can be seen that the cuttings from plants treated with the addition of the fortifier, had a larger size, a greater volume and size of the roots.

(22) In FIG. 2 the appearance of the cuttings treated with addition of fortifier is compared. In FIG. 2a in the front tray and FIG. 2b the right tray (those treated with fortifier) they are compared with the respective example of the untreated cuttings. The cuttings treated with the addition of the fortifier displayed greater vigor and the production and sale of the cuttings was almost a week ahead. In the cuttings treated with the addition of the fortifier there were no losses, while the conventional production of the nursery had losses of between 3 and 10% according to the case, thus with those that had been treated production without losses was achieved. Growth is standardized. It can be concluded that greater volume and size of the cuttings were obtained when treated with the addition of the fortifier.

(23) In FIG. 3 are displayed sizes obtained in centimeters achieved in samples treated with the addition of fortifier FIG. 3a, and without addition FIG. 3b. The groups of cuttings on the left are those treated with the addition of the fortifier. As you can see its height and vigor are greater. With the same growth time, the treated cuttings exceeded the average height of the untreated cuttings by 2 and 3 cm. The difference in size achieved thanks to the use of the fortifier is very evident.

(24) After conducting the culture, a number of cuttings were collected as a representative sample and the biological mass of the mother plants with addition of fortifier and those without was determined. The results obtained are presented in the table below.

(25) Increase in Biological Mass

(26) TABLE-US-00001 Increase, % Average weight of fresh plants, g Conventional mother plant cuttings 9.10 Mother plant cuttings with fortifier 14.20 36.0 Average weight of dry plants, g Conventional mother plant cuttings 1.40 Mother plant cuttings with fortifier 2.20 36.0
Test on Beans of the Santa Pau Variety

(27) The results presented have been in experimentation and testing of the fortifier in bean plants of the Santa Pau variety. The study was conducted with a sample of 143 plants. All the plants were fertilized in the same manner, in this case with a conventional granulated fertilizer that was mixed with the soil. From here, part of the plants were also watered with the fortifying product, leaving the other part as a control treated only with the conventional fertilizer.

(28) Tests were carried out with the addition of the fortifier in different concentrations. The recommended concentration according to the protocols of the product was termed 1.0, corresponding to a concentration of 1.0 ppm Polyamine and, from this, the following lower and upper values were defined: 0.25-0.5-1.5-2.0. The number and weight of the filled pods per plant were counted, and also the number and weight of the beans extracted from the pods per plant.

(29) The following results were obtained:

(30) Table 1 shows the weight of the beans per plant where an optimal increase of 40-44% is obtained compared to the control for a dose value of 1.0.

(31) TABLE-US-00002 TABLE 1 Weight of beans per plant Increase with Dose Weight (grams) regard to control Control 8.3 Treatment dose 0.25 8.9  7% Treatment dose 0.5 10.8 30% Treatment dose 1.0 11.9 44% Treatment dose 2.0 11.6 40%

(32) Table 2 shows the average number of beans per plant, where an optimum increase of 33% is obtained compared to the control for a dose value of 1.0.

(33) TABLE-US-00003 TABLE 2 Average number of beans per plant Increase with Dose No. of beans regard to control Control 34 Treatment dose 0.25 36  5% Treatment dose 0.5 41 21% Treatment dose 1.0 45 33% Treatment dose 1.5 41 20% Treatment dose 2.0 41 22%

(34) Table 3 shows the average weight of the pods per plant where an optimal increase of 44% is obtained compared to the control for a dose value of 1.0.

(35) TABLE-US-00004 TABLE 3 Average weight of pods per plant Increase with Weight (grams) Weight (grams) regard to control Control 10.7 Treatment dose 0.25 11.5  8% Treatment dose 0.5 13.4 25% Treatment dose 1.0 15.4 44% Treatment dose 1.5 13.8 29% Treatment dose 2 14.2 32%

(36) Table 4 shows the average number of pods per plant, where an optimum increase of 24% with respect to the control is obtained for a dose value of 1.0.

(37) TABLE-US-00005 TABLE 4 Average number of pods per plant Dose No. pods Increase in control Control 11 Treatment dose 0.25 12  9% Treatment dose 0.5 13 12% Treatment dose 1.0 14 24% Treatment dose 1.5 13 16% Treatment dose 2 14 22%

(38) Table 5 presents the average weight of each pod, where an optimum increase of 17% is obtained with respect to the control for a dose value of 1.0.

(39) TABLE-US-00006 TABLE 5 Average weight of each pod Dose Weight (grams) Increase in control Control 1.01 Treatment dose 0.25 0.99 −2% Treatment dose 0.5 1.07 12% Treatment dose 1 1.18 17% Treatment dose 1.5 1.09  8% Treatment dose 2 1.07  6%

(40) When applying different concentrations of the fortifier, in general there was a significant increase both in the number and weight of the pods per plant, as well as in the number and weight of the beans per plant.

(41) Ornamental Plant

(42) The objective of the test was to observe the differences in growth and flowering of the conventional production of the nursery with the same conventional production plus addition of a fortifier solution. The study was carried out in an ornamental plant nursery. Currently it is one of the largest producers of ornamental plants in Maresme. 2 varieties of cuttings and 5 varieties of plants were treated.

(43) Cuttings:

(44) Cuttings from Ville de Paris Geraniums and Carnations were treated. In both cases 600 units of each. They were watered 3 times with addition of the fortifier, 1 weekly watering for 3 weeks to solution 1/200 vol/vol. In FIG. 4 two photos of the treated cuttings with addition of the fortifier are shown. In both varieties the result was the same, the growth of the cuttings was advanced. They left the nursery a week earlier compared to the conventional one.

(45) Plants:

(46) A total of 380 plants of different varieties were treated with the addition of fortifier, having 5 weeks of life and 6 applications were made. The following varieties were tested:

(47) Ville de Paris Geranium: 80 units.

(48) Double flower geranium: 25 units.

(49) Carnations: 70 units.

(50) Dimorphotheca: 145 units.

(51) Whorled plectranthus: 30 units.

(52) Zone: 30 units.

(53) The applications were made at dilution 1/200 in the following order:

(54) TABLE-US-00007 1.sup.st month 2.sup.nd month 3.sup.rd month M T W T F S S M T W T F S S M T W T F S S Week 1 1 Week 1 3 Week 1 5 Week 2 2 Week 2 4 Week 2 6 Week 3 Week 3 Week 3 Week 4 Week 4 Week 4

(55) During the test, growth and flowering were evaluated. Between the third and the fourth application the change was already evident. In general, all except the whorled plectranthus displayed greater vigor. These manifested increased vigor and flowering, generally an increase in size of between 10 and 20% depending on the case. They also had more roots and a greater number of flowers. On the other hand, the whorled plectranthus manifested an increase in the number of branches.

(56) Geranium Test.

(57) FIG. 4 shows the geranium cuttings treated with addition of the fortifier, those that overtook conventional nursery production between 5 and 7 days. Its growth was greater and faster.

(58) Dimorphotheca Test.

(59) FIG. 5 shows the results obtained in the treated plants with addition of fortifier, which showed a higher quantity of roots. The plant on the right is the plant treated with the fortifier and as you can see the roots are more numerous and go up higher into the pot. FIG. 6 shows that the treated plants have greater vigor and a greater number of leaves per shoot and buds about to flower.

(60) FIG. 7 shows the action of the fortifier in growing Dimorphotheca plants. On the left in both photos the pot corresponding to the treated plants with addition of fortifier is shown. The application of the product resulted in a larger size of these of between 10 and 20%, with a higher growth rate and vigor. In FIG. 8 on the right in both photos the pot corresponding to plants treated with addition of fortifier is shown. The plants of treated Dimorphothecas display greater vigor and better flowering. Between 10 and 30% more flowers depending on the sample analyzed.

(61) An example of the second embodiment is made by adding 120 liters of filtered and magnetically treated wastewater from brewer's yeast are placed in a 1000 liters container. 100 kg of sardine waste homogenized in 2 l/kg saline solution is added. The container is topped with the saline solution, whilst maintaining the pH of 5. The mix is kept in anaerobic conditions between 20-40 days and at 24-26° C., ensuring that the pH is maintained between 4.5 and 7. The result is filtered with a filter whose pore size is 80 μm and bottled.

SUMMARY

(62) Thus, in the present invention, direct fermentation of wastewater with very low protein concentrations coming from the beer production has been achieved. The use of this waste, by eliminating its release into the environment, leads to the corresponding reduction in environmental impact.

(63) It is noteworthy that the objectives of procurement within the fortifier composition of triamine spermidine and tetramine spermine have also been achieved; thus obtaining an improved fortifier, and evidence of effective action in various crops.

(64) The invention having been sufficiently described, as well as a preferred embodiment thereof, as an example and without limitation, it should be added that it is possible to make changes in its constitution and materials employed without departing from the scope thereof, defined in the following claims.