KEY INTERMEDIATE FOR SYNTHESIS OF PROSTAGLANDIN COMPOUND AND PREPARATION METHOD THEREOF

Abstract

The present invention relates to the technical field of organic chemical engineering, and in particular to a key intermediate for synthesizing prostaglandin compounds and a preparation method therefor. When applied to the synthesis of prostaglandin compounds, the process flow is simplified, the yield and product purity are improved, the production costs are reduced, and the industrial application is easy.

##STR00001##

Claims

1. A key intermediate for synthesis of a prostaglandin compound, having a structure shown below: ##STR00071## wherein denotes a single bond or double bond, and if it is a double bond, R.sup.1 is absent; and wherein R.sup.1 and R.sup.2 are each H or protecting groups; R.sup.3 and R.sup.4 are the same or different alkyl or aryl, or R.sup.3 and R.sup.4 form a ring; and R.sup.5 and R.sup.6 are the same or different H, alkyl, or aryl.

2. The key intermediate for the synthesis of the prostaglandin compound according to claim 1, having a structure shown below: ##STR00072## wherein R.sup.1, R.sup.2, R.sup.3, and R.sup.4 are as defined above.

3. The key intermediate for the synthesis of the prostaglandin compound according to claim 1, which is ##STR00073## wherein R.sup.1, R.sup.2, R.sup.3, and R.sup.4 are as defined above.

4. The key intermediate for the synthesis of the prostaglandin compound according to claim 1, which is ##STR00074## wherein R.sup.1 and R.sup.2 are as defined above, and n is an integer from 1 to 3.

5. The key intermediate for the synthesis of the prostaglandin compound according to claim 1, wherein the intermediate is selected from: ##STR00075## wherein P is a protecting group.

6. The key intermediate for the synthesis of the prostaglandin compound according to claim 1, wherein the protecting group is selected from an ether protecting group, an acyl protecting group, a silyl ether protecting group, an acetal protecting group.

7. The key intermediate for the synthesis of the prostaglandin compound according to claim 1, wherein the intermediate is selected from: ##STR00076##

8. A method for preparing the key intermediate for the synthesis of the prostaglandin compound according to claim 1, comprising steps of: a) asymmetrically reducing compound S1 to obtain chiral alcohol compound S2, and then protecting hydroxyl group of the chiral alcohol compound S2 with silane to obtain Weinreb amide compound S3; b) subjecting the Weinreb amide compound S3 to an addition reaction with an alkyne reagent to obtain enyne compound S4; c) subjecting the enyne compound S4 to a Zhang enyne cycloisomerization to obtain five-membered ring S5; d) conjugating the five-membered ring S5 to reduce double bond thereof to obtain compound S6, and further reducing ketone of the compound S6 to obtain compound S7; and e) deprotecting the compound S7 by removing TIPS thereof to obtain compound S8; referring to the following reaction route: ##STR00077##

9. A method for preparing a prostaglandin compound, comprising preparing the prostaglandin compound by the key intermediate for the synthesis of the prostaglandin compound according to claim 1.

10. The method for preparing the prostaglandin compound according to claim 9, wherein the prostaglandin compound is selected from: ##STR00078## ##STR00079## ##STR00080## ##STR00081## ##STR00082## ##STR00083##

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0046] FIG. 1 shows the structures of a specific intermediate according to the present application and prostaglandin compounds.

DESCRIPTION OF THE EMBODIMENTS

[0047] The present invention will be further described in detail below with reference to examples and accompanying drawings. However, the present invention is not limited thereto.

Example 1

[0048] ##STR00033## ##STR00034##

[0049] Step 1:

##STR00035##

[0050] Hydrogenation step of 1 mmol substrate (S/C=1000): In an argon-filled glove box, f-amphox (5.52 mg, 0.01 mmol) and a metal precursor [Ir(COD)Cl].sub.2 (3.35 mg, 0.05 mmol) were dissolved in anhydrous and anaerobic i-PrOH (1 mL), stirred at room temperature for 1 hr, to obtain a catalyst solution of 10 μmol/mL. In the glove box, Compound S1 (171 mg, 1 mmol) was dissolved in a 20 mL reaction flask containing 10 mL of anhydrous and anaerobic toluene, then MeOK (0.7 mg, 0.01 mmol) was added to the reaction system, and finally 0.1 mL of the fresh catalyst prepared above was added. The reaction flask was transferred to a hydrogenation reactor, and the reactor was purged three times with hydrogen and then introduced with 40 atm of hydrogen. The reaction system was stirred for 24 hrs at room temperature. After the reaction, hydrogen in the reactor was discharged, and the reaction solution was dried by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=2:1, to obtain 150 mg of a liquid product S2 as a light yellow oil (yield 88%).

[0051] Step 2:

##STR00036##

[0052] Compound S2 (1.73 g, 10 mmol) and 10 mL of dry DCM were added to a 25 mL reaction flask, and then 2, 6-lutidine (1.6 g, 15 mmol) was added to the reaction system. Finally, TIPSOTf (3.67 g, 12 mmol) was slowly added dropwise to the reaction solution, and reacted at room temperature for 30 minutes. The reaction was monitored by TLC. After the reaction was completed, water was added to stop the reaction. The reaction solution was extracted with DCM (20 mL*3), and the organic phases were combined with anhydrous sodium sulfate and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=4:1, to obtain 3.28 g of a liquid product S3 as a light yellow oil (yield 99%).

[0053] Step 3:

##STR00037##

[0054] Under an argon atmosphere at −78° C., Compound S3 (294 mg, 3 mmol) and 5 mL of anhydrous THF were added to a 25 mL dry reaction flask, and then 1.25 mL (2.4 M, 3 mmol) of n-butyl lithium was added dropwise to the reaction solution, and reacted at −78° C. for 30 minutes. Then Compound (328 mg, 1 mmol) was dissolved in 2 mL of dry THF, and slowly added dropwise to the above reaction solution. The reaction solution was then immediately heated to −10° C., and continuously reacted for 30 minutes. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=20:1, to obtain 351 mg of a liquid product S4 as a light yellow oil (yield 96%).

[0055] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 5.78 (s, 1H), 5.65-5.56 (m, 1H), 5.43 (dd, J=15.2, 7.3 Hz, 1H), 4.72 (dd, J=13.3, 6.5 Hz, 1H), 4.10-3.99 (m, 2H), 3.99-3.88 (m, 2H), 2.84 (dd, J=14.8, 6.6 Hz, 1H), 2.68 (dd, J=14.8, 6.3 Hz, 1H), 1.64 (d, J=6.3 Hz, 3H), 1.01 (s, 21H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 184.67 (s), 133.21 (s), 126.61 (s), 92.51 (s), 85.37 (s), 82.66 (s), 70.22 (s), 64.56 (s), 54.40 (s), 17.96 (s), 17.90 (s), 17.60 (s), 17.34 (s), 12.26 (s).

[0056] Step 4:

##STR00038##

[0057] In an argon-filled glove box, Compound S4 (3.28 g, 9 mmol) was dissolved in a 100 mL reaction flask containing 50 mL of anhydrous and anaerobic DCE. (S)-BINAP (0.558 g, 0.9 mmol) and [Rh(COD)Cl].sub.2 (0.22 g, 0.45 mmol) were added to the reaction flask and stirred at room temperature for 5 minutes. Finally, AgSbF.sub.6 (0.614 g, 1.8 mmol) was added and continuously reacted at room temperature for 10 minutes. The reaction was monitored by TLC. After the reaction was completed, the reaction solution was dried by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=12:1, to obtain 2.78 g of a liquid product S5 as a light yellow oil (yield 85%).

[0058] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 6.33 (d, J=7.4 Hz, 1H), 5.73-5.69 (m, 1H), 5.69-5.64 (m, 1H), 5.24 (dd, J=29.5, 13.6 Hz, 2H), 4.24 (q, J=6.7 Hz, 1H), 4.05-3.97 (m, 2H), 3.96-3.90 (m, 2H), 3.31 (t, J=6.0 Hz, 1H), 2.72 (dd, J=17.9, 6.4 Hz, 1H), 2.40 (dd, J=17.9, 7.2 Hz, 1H), 1.03 (s, 21H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 203.43 (s), 142.37 (s), 136.27 (s), 135.06 (s), 119.54 (s), 96.84 (s), 72.37 (s), 65.20 (d, J=5.6 Hz), 57.37 (s), 48.52 (s), 17.93 (s), 17.90 (s), 12.13 (s).

[0059] Step 5:

##STR00039##

[0060] Under an argon atmosphere, Compound S5 (366 mg, 1 mmol), anhydrous ZnCl.sub.2 (204 mg, 1.5 mmol), (Ph.sub.3P).sub.4Pd (23.1 mg, 0.02 mmol) and 5 mL anhydrous THF were sequentially added to a 10 mL dry reaction flask. Then Ph.sub.2SiH.sub.2 (312 mg, 1.7 mmol) was added dropwise into the reaction flask, and reacted at 50° C. for 1 hr. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=20:1, to obtain 329 mg of a liquid product S6 as a light yellow oil (yield 90%).

[0061] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 5.84-5.73 (m, 1H), 5.19 (dd, J=21.0, 13.7 Hz, 2H), 5.13 (t, J=5.2 Hz, 1H), 4.22 (q, J=7.2 Hz, 1H), 3.98-3.89 (m, 2H), 3.88-3.79 (m, 2H), 2.69 (dd, J=18.1, 6.8 Hz, 1H), 2.61 (dd, J=18.1, 7.9 Hz, 1H), 2.25 (ddd, J=16.7, 14.7, 6.9 Hz, 2H), 2.06-1.98 (m, 1H), 1.82 (dt, J=12.1, 5.8 Hz, 1H), 1.04 (s, 21H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 215.26 (s), 138.07 (s), 117.66 (s), 102.42 (s), 73.18 (s), 64.80 (s), 64.63 (s), 55.82 (s), 49.36 (s), 47.37 (s), 32.55 (s), 17.95 (d, J=5.5 Hz), 12.16 (s).

[0062] Step 6:

##STR00040##

[0063] Under an argon atmosphere at −78° C., Compound S6 (368 mg, 1 mmol) and 5 mL of anhydrous THF were added to a 25 mL dry reaction flask, and then 1.2 mL (1 M, 1.2 mmol) of L-Selectride was added dropwise into the reaction solution, and reacted at −78° C. for 30 minutes. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=8:1, to obtain 312 mg of a liquid product S7 as a light yellow oil (yield 85%).

[0064] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.60 (dt, J=17.1, 9.6 Hz, 1H), 5.19-4.96 (m, 2H), 4.93 (t, J=4.5 Hz, 1H), 4.17 (td, J=7.2, 2.1 Hz, 1H), 4.06 (dd, J=11.2, 4.9 Hz, 1H), 4.03-3.92 (m, 2H), 3.90-3.79 (m, 2H), 3.04 (d, J=6.4 Hz, 1H), 2.33 (td, J=9.6, 5.2 Hz, 1H), 2.16 (dt, J=13.9, 6.1 Hz, 1H), 2.08-1.92 (m, 1H), 1.82 (dt, J=14.2, 4.0 Hz, 2H), 1.75-1.68 (m, 1H), 1.04 (s, 21H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 139.57 (s), 116.20 (s), 103.90 (s), 78.41 (s), 72.73 (s), 64.97 (s), 64.63 (s), 57.64 (s), 44.94 (s), 43.13 (s), 31.64 (s), 17.97 (s), 12.05 (s).

[0065] Step 7:

##STR00041##

[0066] Compound S7 (370 mg, 1 mmol) and 5 mL of anhydrous THF were added to a 25 mL dry reaction flask, and then 1.5 mL (1 M in THF, 1.5 mmol) of TBAF was added dropwise to the reaction solution, and reacted at room temperature for 30 minutes. The reaction was monitored by TLC. After the reaction was completed, the reaction solution was extracted with Et.sub.2O (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=2:1, to obtain 337 mg of a liquid product S8 as a light yellow oil (yield 91%).

[0067] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.62 (ddd, J=17.1, 10.1, 8.9 Hz, 1H), 5.16-5.01 (m, 2H), 4.89 (dd, J=5.1, 3.0 Hz, 1H), 4.22 (d, J=4.9 Hz, 1H), 4.05-3.93 (m, 2H), 3.92-3.81 (m, 3H), 3.10 (s, 1H), 2.54 (s, 1H), 2.34-2.20 (m, 2H), 1.96-1.82 (m, 2H), 1.79-1.67 (m, 2H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 139.06 (s), 116.58 (s), 103.79 (s), 77.27 (s), 72.33 (s), 65.14 (s), 64.58 (s), 57.59 (s), 44.91 (s), 41.82 (s), 31.15 (s).

Example 2

[0068] ##STR00042##

[0069] At room temperature, Compound S7 (2 g, 5.4 mmol) and 25 mL of THF were added to a 100 mL reaction flask, and then 25 mL of 1N HCl was added dropwise to the reaction solution and reacted at room temperature for 2 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated sodium bicarbonate aqueous solution. The reaction solution was extracted with ethyl acetate (25 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 900 mg of a liquid product S9 as a light yellow oil (yield 98%).

[0070] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.62 (m, 1H), 5.14-5.04 (m, 2H), 4.89 (dd, J=5.1, 3.0 Hz, 1H), 4.22 (t, J=5.1 Hz, 1H), 4.05-3.93 (m, 2H), 3.91-3.80 (m, 3H), 3.10 (br, 1H), 2.54 (br, 1H), 2.33-2.20 (m, 2H), 1.95-1.81 (m, 2H), 1.79-1.68 (m, 2H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 139.1, 116.6, 103.8, 77.3, 72.3, 65.1, 64.6, 57.6, 44.9, 41.8, 31.1. HRMS (ESI) Calcd for C.sub.11H.sub.18O.sub.4Na [M+Na].sup.+: 237.1097, found. 237.1095.

Example 3

[0071] ##STR00043##

[0072] At room temperature, Compound S6 (1.5 g, 4.1 mmol) and 25 mL of THF were added to a 100 mL reaction flask, and then 25 mL of 1N HCl was added dropwise to the reaction solution and reacted at room temperature for 2 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated sodium bicarbonate aqueous solution. The reaction solution was extracted with ethyl acetate (25 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 620 mg of a liquid product S10 as a light yellow oil (yield 90%).

[0073] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 9.73 (s, 1H), 5.76-5.70 (m, 1H), 5.25 (s, 1H), 5.22 (d, J=7.9 Hz, 1H), 4.18 (q, J=8.2 Hz, 1H), 2.84-2.77 (m, 2H), 2.70 (dd, J=18.7, 5.0 Hz, 1H), 2.56-2.45 (m, 2H), 2.41 (dd, J=18.6, 9.4 Hz, 1H), 2.25 (br, 1H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 212.5, 199.3, 136.8, 119.4, 72.0, 55.3, 49.0, 45.2, 41.2. HRMS (ESI) Calcd for C.sub.9H.sub.12O.sub.3Na [M+Na]+: 191.0679, found: 191.0679.

Example 4

[0074] The key intermediate for the synthesis of a prostaglandin compound was reacted with an intermediate represented by the following formulas to prepare the prostaglandin compounds.

[0075] The structures of the intermediate is selected from:

##STR00044##

[0076] The preparation route was as follows:

##STR00045##

Example 5

[0077] The preparation method of a specific intermediate in Example 4 was as follows:

##STR00046##

[0078] Step 1:

##STR00047##

[0079] Hydrogenation step (S/C=1000): In an argon-filled glove box, f-amphox (55.2 mg, 0.1 mmol) and a metal precursor [Ir(COD)Cl].sub.2 (33.5 mg, 0.5 mmol) were dissolved in anhydrous and anaerobic i-PrOH (1 mL), stirred at room temperature for 1 hr, to obtain a catalyst solution of 100 μmol/mL. In the glove box, Compound SC-1 (2.6 g, 20 mmol) was dissolved in a 100 mL reaction flask containing 50 mL of anhydrous and anaerobic isopropanol, then K.sub.2CO.sub.3 (27.8 mg, 0.01 mmol) was added to the reaction system, and finally 0.2 mL of the fresh catalyst prepared above was added. The reaction flask was transferred to a hydrogenation reactor, and the reactor was purged three times with hydrogen and then introduced with 80 atm of hydrogen. The reaction system was stirred 24 hrs at room temperature. After the reaction, hydrogen in the reactor was discharged, and the reaction solution was dried by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=2:1, to obtain 2.55 g of a liquid product SC-2 as a light yellow oil (yield 98%).

[0080] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 3.66 (ddd, J=13.9, 9.2, 3.7 Hz, 2H), 3.42 (dd, J=11.0, 7.7 Hz, 1H), 2.49 (s, 2H), 1.47-1.38 (m, 3H), 1.31 (t, J=10.5 Hz, 5H), 0.88 (t, J=6.7 Hz, 3H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 72.29 (s), 66.60 (s), 32.93 (s), 31.79 (s), 25.24 (s), 22.49 (s), 13.91 (s).

[0081] Step 2:

##STR00048##

[0082] Under an argon atmosphere at −20° C., (Me).sub.3SI (26.8 g, 131.5 mmol) and 100 mL of anhydrous THF were added to a 250 mL dry reaction flask, and then 54 mL (2.4 M, 131.5 mmol) of n-butyl lithium was added dropwise to the reaction solution, and reacted at −20° C. for 30 minutes. Compound SC-3 (3.0, 26.3 mmol) was dissolved in 10 mL of dry THF, and slowly added dropwise to the above reaction solution. The reaction solution was then slowly heated to room temperature, and continuously reacted for 5 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (100 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=20:1, to obtain 2.8 g of a liquid product SC-4 as a light yellow oil (yield 88%).

[0083] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.94-5.79 (m, 1H), 5.15 (ddt, J=43.7, 10.4, 1.4 Hz, 2H), 4.13-4.06 (m, 1H), 1.58-1.48 (m, 3H), 1.35-1.28 (m, 5H), 0.88 (t, J=6.8 Hz, 3H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 141.30 (s), 114.46 (s), 73.23 (s), 36.95 (s), 31.71 (s), 24.97 (s), 22.56 (s), 13.98 (s).

Example 6

[0084] The preparation method of another specific intermediate in Example 4 was as follows:

[0085] Step 1:

##STR00049##

[0086] Hydrogenation step (S/C=500): In an argon-filled glove box, f-amphox (55.2 mg, 0.1 mmol) and a metal precursor [Ir(COD)Cl].sub.2 (33.5 mg, 0.5 mmol) were dissolved in anhydrous and anaerobic i-PrOH (1 mL), and stirred at room temperature for 1 hr, to obtain a catalyst solution of 100 μmol/mL. In the glove box, Compound SC-5-1 (3.28 g, 20 mmol) was dissolved in a 100 mL reaction flask containing 50 mL of anhydrous and anaerobic isopropanol, then K.sub.2CO.sub.3 (27.8 mg, 0.01 mmol) was added to the reaction system, and finally 0.4 mL of the fresh catalyst prepared above was added. The reaction flask was transferred to a hydrogenation reactor, and the reactor was purged three times with hydrogen and then introduced with 50 atm of hydrogen. The reaction system was stirred 24 hrs at room temperature. After the reaction, hydrogen in the reactor was discharged, and the reaction solution was dried by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=2:1, to obtain 3.2 g of a liquid product SC-6-1 as a light yellow oil (yield 98%).

[0087] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.34-7.25 (m, 2H), 7.19 (dd, J=10.6, 4.3 Hz, 3H), 3.72 (tdd, J=7.8, 5.0, 3.1 Hz, 1H), 3.55 (ddd, J=18.8, 11.1, 5.3 Hz, 2H), 2.96-2.62 (m, 4H), 1.84-1.66 (m, 2H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 141.64 (s), 128.42 (s), 128.37 (s), 125.93 (s), 71.52 (s), 66.74 (s), 34.61 (s), 31.76 (s).

[0088] Step 2:

##STR00050##

[0089] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.31-7.23 (m, 2H), 7.17 (dd, J=10.7, 4.4 Hz, 3H), 2.92 (dddd, J=6.5, 5.0, 4.0, 2.8 Hz, 1H), 2.85-2.68 (m, 3H), 2.44 (dd, J=5.0, 2.7 Hz, 1H), 1.92-1.74 (m, 2H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 141.12 (s), 128.29 (s), 128.23 (s), 125.87 (s), 51.60 (s), 47.05 (s), 34.15 (s), 32.10 (s).

[0090] Step 3:

##STR00051##

[0091] Under an argon atmosphere at −20° C., (Me).sub.3SI (30 g, 147 mmol) and 100 mL of anhydrous THF were added to a 250 mL dry reaction flask, and then 61.2 mL (2.4 M, 147 mmol) of n-butyl lithium was added dropwise to the reaction solution, and reacted at −20° C. for 30 minutes. Compound SC-7-1 (4.35, 29.3 mmol) was dissolved in 10 mL of dry THF, and slowly added dropwise to the above reaction solution. The reaction solution was then slowly heated to room temperature, and continuously reacted for 5 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (100 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=20:1, to obtain 4.0 g of a liquid product SC-8-1 as a light yellow oil (yield 85%).

[0092] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.34-7.27 (m, 2H), 7.25-7.18 (m, 3H), 5.92 (ddd, J=17.0, 10.4, 6.2 Hz, 1H), 5.38-5.07 (m, 2H), 4.14 (q, J=6.1 Hz, 1H), 2.85-2.62 (m, 2H), 1.95-1.83 (m, 2H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 141.81 (s), 140.93 (s), 128.41 (s), 128.35 (s), 125.80 (s), 114.91 (s), 72.42 (s), 38.44 (s), 31.57 (s).

Example 7

[0093] The preparation method of another specific intermediate in Example 4 was as follows:

[0094] Step 1:

##STR00052##

[0095] At room temperature, Compound SC-9 (100 g, 617 mmol) and 700 mL of 2-butanone were added to a 1000 mL dry reaction flask, and then epichlorohydrin (143 g, 1.5 mol) and anhydrous potassium carbonate (170 g, 1234 mmol) were added to the reaction solution. The reaction solution was heated to reflux for 12 hrs. Then, water was added to stop the reaction. The reaction solution was extracted with ethyl acetate (200 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=20:1, to obtain 112 g of a liquid product as a light SC-10 yellow oil (512 mmol, yield 85%).

[0096] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 7.36 (t, J=8.0 Hz, 1H), 7.19 (d, J=7.7 Hz, 1H), 7.13 (s, 1H), 7.07 (d, J=8.3 Hz, 1H), 4.26 (dd, J=11.0, 2.7 Hz, 1H), 3.98-3.87 (m, 1H), 3.33 (dt, J=5.8, 2.9 Hz, 1H), 2.86 (dt, J=19.1, 4.4 Hz, 1H), 2.73 (dd, J=4.4, 2.8 Hz, 1H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 170.97 (s), 158.53 (s), 131.76 (q, J=32.2 Hz), 129.96 (s), 123.82 (q, J=272.3 Hz), 118.04 (s), 117.76 (q, J=3.5 Hz), 111.37 (d, J=3.9 Hz), 68.98 (s), 60.22 (s), 49.79 (s), 44.31 (s), 20.81 (s), 14.01 (s).

[0097] Step 2:

##STR00053##

[0098] Under an argon atmosphere at −20° C., (Me).sub.3SI (30 g, 147 mmol) and 100 mL of anhydrous THF were added to a 250 mL dry reaction flask, and then 61.2 mL (2.4 M, 147 mmol) of n-butyl lithium was added dropwise to the reaction solution, and reacted at −20° C. for 30 minutes. Compound SC-10 (6.38, 29.3 mmol) was dissolved in 10 mL of dry THF, and slowly added dropwise to the above reaction solution. The reaction solution was then slowly heated to room temperature, and continuously reacted for 5 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (100 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=20:1, to obtain 6.17 g of a liquid product SC-11 as a light yellow oil (yield 85%).

[0099] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.39 (t, J=8.0 Hz, 1H), 7.23 (d, J=7.7 Hz, 1H), 7.15 (s, 1H), 7.09 (dd, J=8.3, 2.4 Hz, 1H), 5.96 (ddd, J=17.2, 10.6, 5.7 Hz, 1H), 5.47 (dt, J=17.3, 1.4 Hz, 1H), 5.31 (dt, J=10.6, 1.3 Hz, 1H), 4.65-4.54 (m, 1H), 4.05 (dd, J=9.4, 3.4 Hz, 1H), 3.93 (dd, J=9.4, 7.5 Hz, 1H), 2.44 (d, J=3.9 Hz, 1H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 158.54 (s), 135.84 (s), 131.81 (q, J=32.4 Hz), 130.00 (s), 123.84 (q, J=272.3 Hz), 117.99 (s), 117.82 (q, J=3.8 Hz), 117.31 (s), 111.42 (q, J=3.8 Hz), 71.84 (s), 71.03 (s).

Example 8

[0100] The preparation method of another specific intermediate in Example 4 was as follows:

[0101] Step 1:

##STR00054##

[0102] At room temperature, Compound SC-12 (2 g, 15.6 mmol) and 50 mL of 2-butanone were added to a 1000 mL dry reaction flask, and then epichlorohydrin (3.6 g, 39 mol) and anhydrous potassium carbonate (4.3 g, 31.2 mmol) were added to the reaction solution. The reaction solution was heated to reflux for 12 hrs. Then, water was added to stop the reaction. The reaction solution was extracted with ethyl acetate (50 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=20:1, to obtain 2.3 g of a liquid product SC-13 as a light yellow oil (12.6 mmol, yield 81%).

[0103] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.17 (t, J=8.1 Hz, 1H), 6.93 (ddd, J=7.9, 1.9, 0.9 Hz, 1H), 6.90 (t, J=2.2 Hz, 1H), 6.79 (ddd, J=8.4, 2.5, 0.8 Hz, 1H), 4.20 (dd, J=11.0, 2.9 Hz, 1H), 3.85 (dd, J=11.0, 5.9 Hz, 1H), 3.31 (ddt, J=5.7, 4.1, 2.8 Hz, 1H), 2.87 (dd, J=4.8, 4.2 Hz, 1H), 2.71 (dd, J=4.9, 2.7 Hz, 1H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 159.00 (s), 134.62 (s), 130.12 (s), 121.15 (s), 114.85 (s), 112.92 (s), 68.79 (s), 49.74 (s), 44.27 (s).

[0104] Step 2:

##STR00055##

[0105] Under an argon atmosphere at −20° C., (Me).sub.3SI (30 g, 147 mmol) and 100 mL of anhydrous THF were added to a 250 mL dry reaction flask, and then 61.2 mL (2.4 M, 147 mmol) of n-butyl lithium was added dropwise to the reaction solution, and reacted at −20° C. for 30 minutes. Compound SC-13 (5.4 g, 29.3 mmol) was dissolved in 10 mL of dry THF, and slowly added dropwise to the above reaction solution. The reaction solution was then slowly heated to room temperature, and continuously reacted for 5 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (100 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=20:1, to obtain 4.95 g of a liquid product SC-14 as a light yellow oil (yield 85%).

[0106] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.19 (t, J=8.1 Hz, 1H), 6.95 (ddd, J=7.9, 1.9, 0.8 Hz, 1H), 6.91 (t, J=2.2 Hz, 1H), 6.80 (ddd, J=8.4, 2.4, 0.8 Hz, 1H), 5.93 (ddd, J=17.2, 10.6, 5.6 Hz, 1H), 5.45 (dt, J=17.3, 1.4 Hz, 1H), 5.29 (dt, J=10.6, 1.3 Hz, 1H), 4.66-4.49 (m, 1H), 3.99 (dd, J=9.4, 3.5 Hz, 1H), 3.87 (dd, J=9.4, 7.6 Hz, 1H), 2.58 (d, J=3.7 Hz, 1H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 159.10 (s), 135.80 (s), 134.83 (s), 130.23 (s), 121.36 (s), 117.31 (s), 115.01 (s), 113.03 (s), 71.80 (s), 71.00 (s).

Example 9

[0107] Preparation of Prostaglandin Compound PGF.sub.2α

##STR00056##

[0108] Step 1:

##STR00057##

[0109] In an argon-filled glove box, Compound S8 (22 mg, 0.1 mmol), SC-4 (65.5 mg, 0.5 mmol) and second-generation Hovedy-Grubbs catalyst (6.2 mg, 0.01 mmol) were dissolved in anhydrous and anaerobic DCM (2 mL). Then the reaction system was heated to 60° C. and stirred for 5 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction solution was dried by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 27 mg of a liquid product S11 as a light yellow oil (yield 95%), and 6 mg of raw material was recovered.

[0110] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 5.55 (dd, J=15.2, 7.3 Hz, 1H), 5.41 (dd, J=15.2, 9.1 Hz, 1H), 4.89 (t, J=4.0 Hz, 1H), 4.20 (s, 1H), 4.07-3.98 (m, 2H), 3.96 (dd, J=10.8, 6.3 Hz, 1H), 3.86 (ddt, J=18.7, 12.2, 6.2 Hz, 3H), 3.13 (s, 2H), 2.44 (s, 1H), 2.37-2.28 (m, 1H), 2.25 (dd, J=17.1, 9.8 Hz, 1H), 1.92 (s, 1H), 1.87-1.81 (m, 2H), 1.73-1.64 (m, 2H), 1.59-1.51 (m, 1H), 1.50-1.41 (m, 1H), 1.39-1.23 (m, 6H), 0.87 (t, J=6.7 Hz, 3H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 136.01 (s), 132.15 (s), 103.81 (s), 77.15 (s), 72.96 (s), 71.96 (s), 65.16 (s), 64.61 (s), 55.82 (s), 44.87 (s), 41.80 (s), 37.20 (s), 31.70 (s), 31.07 (s), 25.19 (s), 22.57 (s), 13.99 (s).

[0111] Step 2:

##STR00058##

[0112] Compound S1l (35 mg, 0.11 mmol), 5 mL of THF and 1 mL of deionized water were added to a 25 mL reaction flask, and then TsOH (1.7 mg, 0.01 mmol) was added to the reaction solution in one portion, and reacted at 75° C. for 3 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated sodium bicarbonate aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product S12 was directly used in the next reaction. Under an argon atmosphere at −78° C., Compound S13 (243 mg, 0.55 mmol) and 5 mL of anhydrous THF were added to a 25 mL dry reaction flask, and then 1.1 mL (1 M, 1.1 mmol) of i-butyl lithium was added dropwise to the reaction solution, and reacted at −78° C. for 30 minutes. Then the freshly prepared compound S12 above was dissolved in 2 mL of dry THF, and slowly added dropwise to the above reaction solution. The reaction solution was then heated to room temperature, and continuously reacted for 2 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 21 mg of a liquid product PG.sub.2α as a light yellow oil (yield of two steps: 55%).

[0113] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 5.56 (dd, J=15.3, 7.0 Hz, 1H), 5.46 (ddd, J=19.9, 14.7, 8.0 Hz, 2H), 5.40-5.30 (m, 1H), 4.29 (s, 3H), 4.16 (s, 1H), 4.08 (dt, J=16.4, 8.2 Hz, 1H), 3.94 (s, 1H), 2.33 (t, J=6.4 Hz, 3H), 2.28-2.17 (m, 2H), 2.11 (dd, J=13.1, 6.9 Hz, 3H), 1.74 (d, J=14.6 Hz, 1H), 1.67 (dd, J=16.9, 13.0 Hz, 2H), 1.61-1.53 (m, 1H), 1.46 (dt, J=26.4, 8.9 Hz, 2H), 1.39-1.22 (m, 8H), 0.88 (t, J=6.7 Hz, 3H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 134.91 (s), 132.63 (s), 129.61 (s), 129.16 (s), 77.68 (s), 73.12 (s), 72.57 (s), 55.38 (s), 50.30 (s), 42.72 (s), 36.94 (s), 32.99 (s), 31.71 (s), 26.27 (s), 25.22 (s), 24.51 (s), 22.61 (s), 14.02 (s).

Example 10

[0114] Preparation of Prostaglandin Compound Travoprost:

##STR00059##

[0115] Step 1:

##STR00060##

[0116] In an argon-filled glove box, Compound S8 (43 mg, 0.2 mmol), SC-11 (243 mg, 1.0 mmol) and second-generation Hovedy-Grubbs catalyst (12 mg, 0.02 mmol) were dissolved in anhydrous and anaerobic DCM (2 mL). Then the reaction system was heated to 60° C. and stirred for 5 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction solution was dried by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 68 mg of a liquid product S14 as a light yellow oil (yield 82%).

[0117] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.36 (t, J=8.0 Hz, 1H), 7.19 (d, J=7.7 Hz, 1H), 7.13 (s, 1H), 7.06 (dd, J=8.3, 2.0 Hz, 1H), 5.73-5.62 (m, 2H), 4.87 (dd, J=4.7, 3.1 Hz, 1H), 4.51 (d, 1=3.4 Hz, 1H), 4.20 (s, 1H), 4.06-3.75 (m, 7H), 3.37-3.18 (m, 3H), 2.29 (dt, J=8.0, 6.8 Hz, 2H), 1.95-1.78 (m, 2H), 1.77-1.65 (in, 2H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 158.59 (s), 133.88 (s), 131.76 (q, J=32.3 Hz), 130.08 (s), 129.98 (s), 123.84 (q, J=272.3 Hz), 117.94 (s), 117.70 (q, J=3.6 Hz), 111.51 (q, J=3.8 Hz), 103.66 (s), 77.09 (s), 72.01 (d, J=3.6 Hz), 70.19 (s), 65.10 (s), 64.54 (s), 55.79 (s), 44.82 (s), 41.83 (s), 30.98 (s).

[0118] Step 2:

##STR00061##

[0119] Compound S14 (46 mg, 0.11 mmol), 5 mL of THF and 1 mL of deionized water were added to a 25 mL reaction flask, and then TsOH (1.9 mg, 0.015 mmol) was added to the reaction solution in one portion, and reacted at 75° C. for 3 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated sodium bicarbonate aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained S15 crude product was directly used in the next reaction. Under an argon atmosphere at −78° C., Compound S13 (243 mg, 0.55 mmol) and 5 mL of anhydrous THF were added to a 25 mL dry reaction flask, and then 1.1 mL (1 M, 1.1 mmol) of n-butyl lithium was added dropwise to the reaction solution, and reacted at −78° C. for 30 minutes. Then the freshly prepared compound S15 above was dissolved in 2 mL of dry THF, and slowly added dropwise to the above reaction solution. The reaction was then heated to room temperature, and continuously reacted for 2 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 40 mg of a liquid product Fluprostenol as a light yellow oil (yield of two steps: 80%).

[0120] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 7.40 (t, J=8.0 Hz, 1H), 7.23 (d, J=7.7 Hz, 1H), 7.15 (s, 1H), 7.09 (dd, J=8.3, 2.3 Hz, 1H), 5.71 (ddd, J=21.7, 15.3, 7.6 Hz, 2H), 5.52 (dd, J=17.7, 7.7 Hz, 1H), 5.40 (dd, J=18.3, 7.8 Hz, 1H), 4.60 (td, J=7.2, 3.3 Hz, 1H), 4.24 (s, 1H), 4.09-3.95 (m, 3H), 2.77 (s, 4H), 2.43-2.23 (m, 5H), 2.20-2.04 (m, 5H), 1.82 (d, J=14.7 Hz, 1H), 1.66 (ddt, J=26.6, 13.7, 7.0 Hz, 2H), 1.55-1.48 (m, 1H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 158.49 (s), 135.95 (s), 131.95 (q, J=32.2 Hz), 130.10 (s), 129.84 (s), 128.81 (s), 128.78 (s), 126.66-120.94 (m), 118.08 (s), 117.99 (q, J=3.5 Hz), 111.51 (d, J=3.6 Hz), 78.00 (s), 72.86 (s), 71.82 (s), 71.18 (s), 56.33 (s), 51.17 (s), 43.17 (s), 32.56 (s), 29.69 (s), 26.12 (s), 25.07 (s), 24.49 (s).

Example 11

[0121] Method for preparing Travoprost with Compound Fluprostenol:

##STR00062##

[0122] Fluprostenol (20 mg, 0.043 mmol) and 2 mL of DMF were added to a 25 mL reaction flask, and then CsCO.sub.3 (21.1 mg, 0.065 mmol) was added to the reaction solution in one portion, and reacted at room temperature for 24 hrs. The reaction was monitored by TLC. After the reaction was completed, water was added to stop the reaction. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=8:1, to obtain 18 mg of a liquid product Travoprost as a light yellow oil (yield 86%).

[0123] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.39 (t, J=8.0 Hz, 1H), 7.22 (d, J=7.7 Hz, 1H), 7.15 (s, 1H), 7.09 (dd, J=8.3, 2.2 Hz, 1H), 5.83-5.62 (m, 2H), 5.52-5.34 (m, 2H), 4.99 (hept, J=6.3 Hz, 1H), 4.54 (dd, J=10.8, 5.3 Hz, 1H), 4.21 (d, J=2.9 Hz, 1H), 4.07-3.89 (m, 3H), 2.71 (d, J=3.7 Hz, 1H), 2.48 (d, J=6.5 Hz, 1H), 2.40 (ddd, J=10.4, 8.6, 4.7 Hz, 1H), 2.35-2.24 (m, 4H), 2.21-2.04 (m, 4H), 1.82 (dd, J=14.5, 2.0 Hz, 1H), 1.73-1.62 (m, 3H), 1.57 (ddd, J=14.8, 10.3, 4.4 Hz, 1H), 1.22 (dt, J=6.3, 3.0 Hz, 6H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 173.59 (s), 158.62 (s), 134.86 (s), 131.89 (q, J=32.3 Hz), 130.04 (s), 129.89 (s), 129.34 (s), 128.93 (s), 123.86 (q, J=272.4 Hz), 118.05 (s), 117.84 (dd, J=7.7, 3.8 Hz), 111.45 (q, J=3.8 Hz), 78.25 (s), 73.11 (s), 72.10 (s), 70.67 (s), 67.76 (s), 56.16 (s), 50.76 (s), 42.98 (s), 33.92 (s), 26.57 (s), 25.57 (s), 24.81 (s), 21.81 (s).

Example 12

[0124] Preparation of Prostaglandin Compound Cloprostenol:

[0125] The reaction route was shown below:

##STR00063##

[0126] Step 1:

##STR00064##

[0127] In an argon-filled glove box, Compound S8 (22 mg, 0.1 mmol), SC-14 (97 mg, 0.5 mmol) and second-generation Hovedy-Grubbs catalyst (6 mg, 0.01 mmol) were dissolved in anhydrous and anaerobic DCM (2 mL). Then the reaction system was heated to 60° C. and stirred for 5 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction solution was dried by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 35 mg of a liquid product S16 as a light yellow oil (yield 86%).

[0128] 1H NMR (600 MHz, CDCl.sub.3) δ 7.17 (q, J=7.8 Hz, 1H), 6.96-6.85 (m, 2H), 6.79 (dd, J=8.3, 2.3 Hz, 1H), 5.73-5.58 (m, 2H), 4.88 (dd, J=5.0, 2.9 Hz, 1H), 4.57-4.44 (m, 1H), 4.21 (s, 1H), 4.04-3.98 (m, 1H), 3.98-3.92 (m, 2H), 3.90-3.81 (m, 4H), 3.24 (d, J=35.0 Hz, 1H), 3.10 (s, 2H), 2.30 (qd, J=14.1, 8.5 Hz, 2H), 1.97-1.80 (m, 2H), 1.78-1.67 (m, 2H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 159.19 (s), 134.81 (s), 133.88 (s), 130.22 (s), 130.06 (s), 121.26 (s), 115.10 (s), 113.03 (s), 103.69 (s), 77.14 (s), 72.09 (s), 71.99 (s), 70.23 (s), 65.12 (s), 64.57 (s), 55.86 (s), 44.89 (s), 41.84 (s), 31.03 (s).

[0129] Step 2:

##STR00065##

[0130] Compound S16 (45 mg, 0.11 mmol), 5 mL of THF and 1 mL of deionized water were added to a 25 mL reaction flask, and then TsOH (2.58 mg, 0.015 mmol) was added to the reaction solution in one portion, and reacted at 75° C. for 3 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated sodium bicarbonate aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product S17 was directly used in the next reaction. Under an argon atmosphere at −78° C., Compound S13 (243 mg, 0.55 mmol) and 5 mL of anhydrous THF were added to a 25 mL dry reaction flask, and then 1.1 mL (1 M, 1.1 mmol) of n-butyl lithium was added dropwise to the reaction solution, and reacted at −78° C. for 30 minutes. Then the freshly prepared compound S17 above was dissolved in 2 mL of dry THF, and slowly added dropwise to the above reaction solution. The reaction solution was then heated to room temperature, and continuously reacted for 2 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 34 mg of a liquid product Cloprostenol as a light yellow oil (yield of two steps: 69%).

[0131] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 7.20 (t, J=8.1 Hz, 1H), 6.97-6.94 (m, 1H), 6.92 (s, 1H), 6.81 (dd, J=8.3, 2.3 Hz, 1H), 5.69 (ddd, J=21.6, 15.2, 7.5 Hz, 2H), 5.52 (dd, J=17.7, 7.4 Hz, 1H), 5.39 (dd, J=18.0, 7.9 Hz, 1H), 4.62-4.53 (m, 1H), 4.23 (s, 1H), 4.10-3.96 (m, 2H), 3.93 (dd, J=22.7, 14.6 Hz, 1H), 2.82 (d, J=6.4 Hz, 4H), 2.36 (dd, J=18.9, 14.2 Hz, 3H), 2.30-2.23 (m, 1H), 2.21-2.05 (m, 4H), 1.81 (d, J=14.4 Hz, 1H), 1.66 (tt, J=20.8, 6.8 Hz, 2H), 1.50 (dd, J=12.7, 8.8 Hz, 1H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 177.21 (s), 159.18 (s), 134.92 (s), 134.83 (s), 130.28 (s), 129.65 (s), 129.52 (s), 128.97 (s), 121.32 (s), 115.12 (s), 113.10 (s), 77.56 (s), 72.50 (s), 71.78 (s), 70.72 (s), 55.49 (s), 50.52 (s), 42.86 (s), 32.82 (s), 26.20 (s), 25.04 (s).

Example 13

[0132] Preparation of prostaglandin compound Latanoprost

##STR00066##

[0133] Step 1:

##STR00067##

[0134] In an argon-filled glove box, Compound S8 (48 mg, 0.2 mmol), SC-8-1 (162 mg, 0.5 mmol) and second-generation Hovedy-Grubbs catalyst (12.5 mg, 0.02 mmol) were dissolved in anhydrous and anaerobic DCM (2 mL). Then the reaction system was heated to 60° C. and stirred for 5 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction solution was dried by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 54 mg of a liquid product S18 as a light yellow oil (yield 78%).

[0135] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.27 (dd, J=9.0, 5.9 Hz, 2H), 7.21-7.14 (m, 2H), 5.73-5.54 (m, 1H), 5.50-5.31 (m, 1H), 4.89 (t, J=3.8 Hz, 1H), 4.20 (s, 1H), 4.12-3.92 (m, 3H), 3.92-3.74 (m, 3H), 3.34 (d, J=58.2 Hz, 1H), 3.20 (s, 1H), 2.85 (s, 1H), 2.78-2.60 (m, 2H), 2.39-2.30 (m, 1H), 2.26 (dd, J=17.4, 9.9 Hz, 1H), 2.05 (s, 1H), 1.96-1.75 (m, 4H), 1.69 (ddt, J=15.2, 9.9, 4.9 Hz, 2H). .sup.13C NMR (101 MHz, CDCl.sub.3) δ 141.91 (s), 135.97 (s), 132.74 (s), 128.42 (s), 128.29 (s), 125.71 (s), 103.68 (s), 76.76 (s), 72.24 (s), 71.56 (s), 65.10 (s), 64.57 (s), 55.43 (s), 44.36 (s), 41.74 (s), 38.66 (s), 31.80 (s), 30.84 (s).

[0136] Step 2:

##STR00068##

[0137] Compound S18 (28 mg, 0.08 mmol), 10 mg of palladium on carbon, 2 mL of ethanol and 40 μl of sodium hydroxide aqueous solution (1.0 M, 40 μmol) were added to a 25 mL reaction flask, the reactor was purged three times with hydrogen and then introduced with 1 atm of hydrogen. The reaction was continued at room temperature for 5 hrs. The reaction was monitored by TLC. After the reaction was completed, the palladium on carbon was filtered off, and the solvent was removed by rotary evaporation. The obtained crude product S19 was directly used in the next reaction.

[0138] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 7.20 (t, J=7.4 Hz, 2H), 7.15-7.08 (m, 3H), 4.84 (dd, J=13.3, 9.5 Hz, 1H), 4.15 (s, 1H), 3.99-3.86 (m, 2H), 3.86-3.74 (m, 3H), 3.65-3.51 (m, 1H), 3.13 (s, 2H), 2.81-2.66 (m, 1H), 2.65-2.52 (m, 1H), 1.94-1.78 (m, 4H), 1.71 (ddd, J=23.1, 11.7, 6.9 Hz, 2H), 1.66-1.58 (m, 1H), 1.56-1.45 (m, 3H), 1.38 (tt, J=19.2, 9.6 Hz, 1H), 1.29 (ddd, J=22.1, 14.6, 7.4 Hz, 1H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 142.08 (s), 128.37 (s), 128.36 (s), 125.75 (s), 103.81 (s), 78.17 (s), 74.35 (s), 71.20 (s), 65.10 (s), 64.58 (s), 53.32 (s), 46.83 (s), 41.66 (s), 39.04 (s), 35.57 (s), 32.86 (s), 32.07 (s), 29.35 (s).

Example 14

[0139] Preparation of prostaglandin compound Latanoprost

[0140] Step 1:

##STR00069##

[0141] Compound S19 (54 mg, 0.15 mmol), 5 mL of THF and 1 mL of deionized water were added to a 25 mL reaction flask, and then TsOH (2.5 mg, 0.015 mmol) was added to the reaction solution in one portion, and reacted at 75° C. for 3 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated sodium bicarbonate aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product S20 was directly used in the next reaction. Under an argon atmosphere at −78° C., Compound S13 (332 mg, 0.75 mmol) and 5 mL of anhydrous THF were added to a 25 mL dry reaction flask, and then 1.5 mL (1 M, 1.5 mmol) of n-butyl lithium was added dropwise to the reaction solution, and reacted at −78° C. for 30 minutes. Then the freshly prepared compound S20 above was dissolved in 2 mL of dry THF, and slowly added dropwise to the above reaction solution. The reaction solution was then heated to room temperature, and continuously reacted for 2 hrs. The reaction was monitored by TLC. After the reaction was completed, the reaction was terminated by adding a saturated ammonium chloride aqueous solution. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 32 mg of a liquid product Latanoprost acid as a light yellow oil (yield of two steps: 55%).

[0142] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 7.35-7.24 (m, 2H), 7.26-7.12 (m, 3H), 5.59-5.45 (m, 1H), 5.45-5.26 (m, 1H), 4.14 (d, J=23.8 Hz, 2H), 3.98-3.90 (m, 2H), 3.74-3.62 (m, 1H), 2.86-2.74 (m, 1H), 2.65 (ddd, J=19.4, 15.6, 9.5 Hz, 1H), 2.34 (dd, J=14.5, 7.8 Hz, 2H), 2.30-2.21 (m, 2H), 2.15 (td, J=14.9, 7.6 Hz, 2H), 1.93-1.82 (m, 2H), 1.81-1.74 (m, 2H), 1.73-1.65 (m, 3H), 1.61 (dd, J=13.8, 7.2 Hz, 2H), 1.53 (ddd, J=16.2, 12.0, 7.1 Hz, 1H), 1.40-1.30 (m, 2H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 177.26 (s), 142.05 (s), 129.45 (d, J=8.5 Hz), 128.40 (s), 125.82 (s), 78.55 (s), 74.43 (s), 71.52 (s), 52.39 (s), 51.66 (s), 42.48 (s), 38.73 (s), 35.19 (s), 33.04 (s), 32.08 (s), 29.04 (s), 26.63 (s), 26.35 (s), 24.63 (s).

[0143] Step 2:

##STR00070##

[0144] Latanoprost acid (30 mg, 0.076 mmol) and 2 mL of DMF were added to a 25 mL reaction flask, and then CsCO.sub.3 (37 mg, 0.114 mmol) was added to the reaction solution in one portion. The reaction was continued at room temperature for 24 hrs. The reaction was monitored by TLC. After the reaction was completed, water was added to stop the reaction. The reaction solution was extracted with ethyl acetate (5 mL*3), and the organic phases were combined, and dried with anhydrous sodium sulfate by rotary evaporation. The obtained crude product was subjected to silica gel column chromatography with eluent of petroleum ether:ethyl acetate=1:1, to obtain 24 mg of a liquid product Latanoprost as a light yellow oil (yield 73%).

[0145] .sup.1H NMR (600 MHz, CDCl.sub.3) δ 7.24-7.18 (m, 2H), 7.17-7.08 (m, 3H), 5.39 (dt, J=10.8, 7.2 Hz, 1H), 5.31 (dt, J=18.0, 7.1 Hz, 1H), 5.02-4.88 (m, 1H), 4.09 (s, 1H), 3.89 (d, J=23.0 Hz, 1H), 3.66-3.54 (m, 1H), 2.77-2.68 (m, 1H), 2.66-2.57 (m, 1H), 2.44 (s, 1H), 2.29-2.19 (m, 3H), 2.14 (ddd, J=15.9, 11.5, 5.7 Hz, 1H), 2.06 (tq, J=14.5, 7.2 Hz, 2H), 1.85-1.75 (m, 3H), 1.75-1.68 (m, 2H), 1.65-1.58 (m, 3H), 1.57-1.51 (m, 2H), 1.50-1.42 (m, 1H), 1.36-1.24 (m, 2H), 1.19-1.11 (m, 6H). .sup.13C NMR (151 MHz, CDCl.sub.3) δ 173.46 (s), 142.09 (s), 129.56 (s), 129.34 (s), 128.38 (s), 125.79 (s), 78.75 (s), 74.65 (s), 71.29 (s), 67.64 (s), 52.85 (s), 51.86 (s), 42.51 (s), 39.04 (s), 35.78 (s), 34.05 (s), 32.10 (s), 29.61 (s), 26.88 (s), 26.62 (s), 24.92 (s), 21.81 (s).

[0146] Referring to the aforementioned synthetic route, the intermediates can be used in the preparation of compounds shown in FIG. 1.

[0147] Preferred embodiments of the present invention have been described above. However, the present invention is not limited thereto. Any other changes, modifications, alternatives, combinations, simplifications made without departing from the spirit and principle of the present invention are all equivalent replacements, and fall in the protection scope of the present invention.