Composition and Method Containing Pea and Quinoa Natural Peptides And Niacinamide to Enhance Skin Glow, Radiance And Even Skin Tone
20220249347 · 2022-08-11
Assignee
Inventors
- Pushker Sona (Denville, NJ, US)
- Dr. Mihaela Gorcea (Denville, NJ, US)
- Svetlana Gorelik (Denville, NJ, US)
Cpc classification
A61K8/675
HUMAN NECESSITIES
International classification
Abstract
Healthy glow is characterized by radiant skin, brighter, lighter, even skin tone and clear skin without blemishes. Skin pigmentation is a process known as melanogenesis involving production, transportation, and distribution of melanin from the epidermis's inner layer to the skin's surface. There are multiple biological processes and underlying gene pathways that impact overall melanogenesis. Disclosed is a composition and methods for a combination of Vitamin B3 and Pea and Quinoa peptides obtained from hydrolyzed proteins. This composition was found to offer a holistic approach to the skin glow through multi targeted action controlling key biological pathways. Vitamin B3, Pea peptides, and Quinoa peptides were found to deliver a synergistic effect for desired skin glow, radiance, even skin tone, uniformity, homogeneity, smoothing skin texture, reducing dark spots/hyperpigmentation and improve skin appearance without any reported side effects as compared to formulations without the composition.
Claims
1. A skin care composition for skin glow, radiance, and skin tone, comprising the following components: a first component having an effective amount by weight of quinoa (chenopodium quinoa) peptides in a range of 0.0001%-25%; a second component having an effective amount by weight of pea (pisum sativum) peptides in a range of 0.0001%-50%; and a third component having an effective amount by weight of niacinamide in a range of 0.001-70%, wherein the first, the second, and the third components form a composition that has an effect on an epidermis of a user and lightens the epidermis for skin glow, radiance, and skin tone.
2. The composition of claim 1, wherein the effective amount of niacinamide is between 10-50% by weight, the effective amount of pea peptides is 10-50% by weight, and the effective amount of quinoa peptides is 1-10% by weight.
3. The composition of claim 2, further including an amount by weight of water, and a preservative.
4. The composition of claim 3, wherein the amount of water by weight is less than 100%, and the preservative is 0.5-2%.
5. The composition of claim 4, wherein the preservative includes Benzyl Alcohol, Water, Potassium Sorbate, and Sodium Benzoate.
6. The composition of claim 1, wherein the effect on the epidermis is immediately after application of the composition and enhancement of skin glow, skin radiance, even skin tone, skin complexion, skin homogeneity, uniformity, reduction of dark spots; and hyper-pigmentation is visibly observed after only 7 days of treatment and the composition continues to provide the effect after 28 days of treatment.
7. The composition of claim 1, wherein each component provides multi-targeted biological mechanism of action on skin and work via complementary synergistic mechanisms; and the effective amount of Pea peptides inhibit melanin transfer from melanocytes to keratinocytes, reduces dark spots/hyperpigmentation; the effective amount of Quinoa peptides inhibit melanin transfer from melanocytes to keratinocytes and reduces skin pigmentation; and the effective amount of the niacinamide controls melanin distribution and dispersion in the epidermis at its surface to enhance skin lightening, brightness, and even skin tone.
8. The composition of claim 1, wherein the composition provides a multi-targeted biological mechanism of action and provides a biological effect across all of the epidermis layers, from skin surface, stratum corneum, and viable epidermis.
9. The composition of claim 1, wherein the first component, the second component, and the third component combined is clinically proven in in vitro skin models and in vivo human skin to enhance skin glow, radiance, even skin tone, homogeneity, uniformity, and smooth skin texture to improve skin appearance after only 7 days and 28 days of treatment as compared to the components individually, or only two components combined.
10. The composition of claim 1, wherein the first, the second, and the third components are incorporated in a cosmetic formulation for face, body, and hands, and the cosmetic formulation is selected from a group consisting of a cream, a lotion, a serum, a mask, a spray, a toner, a gel, a cream gel, a hydrogel, a vanishing cream, a cold cream, a foundation, a primer, a color cosmetic, a balm, a cleanser, a bath gel, a face cleanser, and any combination thereof.
11. The composition of claim 1, further including a fourth component of water less than 70% by weight to form a Phase D.
12. The composition of claim 11, further including a Phase A, a Phase B, a Phase C, and a Phase E, and further comprises: the Phase A having water, glycerin and tetrasodium glutamate diacetate; the Phase B having stearic acid, cetyl alcohol, hydrogenated ethylhexyl olivate, hydrogenated olive oil, unsaponifiables, isopropyl myristate, titanium dioxide, aluminum hydroxide, stearic acid, tocopheryl acetate, ethylhexyl methoxycinnamae, and butyl methoxydibenzoylmethane; the Phase C having water and potassium hydroxide; and the Phase E having phenoxyethanol.
13. The composition of claim 12, wherein the Phase A is between 0.1-67%, the Phase B is between 0.1-20% the Phase C is between 0.1-5.5%, the Phase D is between 0.1-10%, and the Phase E is between 0.1-1%.
14. The composition of claim 13, wherein the Phase D is 5%.
15. A method of using a composition for skin glow, radiance and even skin tone comprising: topically applying to an epidermis of a user a composition that includes a first component having an effective amount by weight of Quinoa peptides in a range of 0.0001%-25%, a second component having an effective amount by weight of Pea peptides in a range of 0.0001%-25%, and a third component having an effective amount of niacinamide in a range of 0.001-70%, wherein the composition lightens the epidermis of a user for skin glow, radiance and even skin tone.
16. The method of claim 15, wherein the topically applying step further includes applying the composition once a day continuously for at least 7 days for visible observation of an effect on the epidermis after application of the composition and enhancement of skin glow, skin radiance, even skin tone, skin complexion, skin homogeneity, uniformity, reduction of dark spots, and hyperpigmentation.
17. The method of claim 16, further includes using the composition as multifunctional facial, body, and hands cosmetic selected from a group consisting of a skin lightening, a skin brightener, a glow enhancers, a vanishing cream, an oil in water (O/W) emulsion, a cold cream, a lightening brightening glow anti-aging cosmetic formulation, an emulsion, a cream, a lotion, a serum, a mask, a spray, a toner, a gel, a foundation, a beauty balm (BB) cream, a color correcting (CC) cream, and a dynamic do-all or daily defense (DD) cream, a concealer, a make-up primer, and any combination thereof.
18. A method of making a skin care composition for skin glow, radiance, and skin tone, comprising: adding deionized (DI) water into a main tank, and mixing; adding to the main tank a second component having an effective amount by weight of Pea peptides in a range of 0.0001%-25%, followed adding by a first component having an effective amount by weight of Quinoa peptides in a range of 0.0001%-25%. adding into the main tank a third component having an effective amount of niacinamide in a range of 0.001-70% and dissolving all components gradually so that none of the components sink to bottom of the tank to form a composition; and wherein the composition lightens an epidermis of a user for skin glow, radiance and even skin tone.
19. The method of claim 18, further includes adding into the main tank a preservative having an effective amount of phenoxyethanol in a range 0.1-1% after all the components dissolve and mixing the preservative until the preservative is uniformly dispersed to for a mixture.
20. The method of claim 19, further includes maintaining the mixture at a pH from 6.0-8.0.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
[0034] The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
[0035] So that those having ordinary skill in the art will have a better understanding of how to make and use the disclosed composition and methods, reference is made to the accompanying figures wherein:
[0036]
[0037]
[0038]
[0039]
[0040]
[0041]
[0042]
[0043]
[0044]
[0045]
[0046]
DETAILED DESCRIPTION
[0047] The present disclosure is directed to a new composition, and method of making and using a novel combination of natural peptides and niacinamide (Vitamin B3) that is efficacious for skin glow, radiance, skin tone and the like. The present disclosure differs, in one aspect, from current methodologies in that it uses unique natural protein combinations with niacinamide. This combination produced unexpected effects never seen before, and never seen with just using the ingredients of the composition alone. The composition does not further aggravate any side effects of the individual ingredients such as niacinamide that is known to cause skin flushing or tingling.
Combination of Ingredients
[0048] This novel Skin Composition contains two natural peptides, Pea and Quinoa, which work via complementary biological mechanism of action in skin, combined at an optimal ratio with the skin lightening third component of an active niacinamide.
[0049] This novel Skin Composition contains natural Pea peptides which were incorporated because it provides unexpected benefits in skin pigmentation and reduces dark spots/hyperpigmentation, increases skin glow, radiance, brightness, luminosity, enhances even skin tone, homogeneity and uniformity of skin complexion, and improves skin's healthy appearance providing overall anti-aging benefits. These results were unexpected because traditionally the incorporation of natural proteins and peptides in cosmetic formulations and their application to skin are generally used to moisturize, hydrate, nourish the skin and improve the overall health of the skin.
[0050] In vitro gene expression results clearly demonstrated that the Pea peptides provided a significant effect on skin pigmentation, increasing skin glow, brightness, radiance, reducing dark spots/hyperpigmentation providing an even skin tone and a uniform complexion. The present investigators discovered and demonstrated in gene expression testing assays that this natural Pea peptide component downregulates the expression of nine specific genes (biomarkers) in in vitro 3D Asian skin equivalent models, and inhibits skin melanogenesis multiple biological pathways, reduces skin pigmentation, reduces dark spots/hyperpigmentation, inhibits melanosome transfer and formation, reduces solar lentigines, UV spots, blemishes, red freckles to provide an even skin tone, even complexion and a healthy skin. In addition, this component significantly reduced melanin content in in vitro B16 melanoma cells compared to untreated, showing significant skin lightening, brightening effect in cultured cells.
[0051] While conducting and evaluating additional in vitro results obtained from B16 melanoma cultured cells, it was discovered a different and complementary natural peptide such as Quinoa peptides reduced, significantly, the melanin content in B16 melanoma cells, and provided unexpected effects on skin pigmentation, reducing skin pigmentation, increasing skin radiance, glow, lightness.
[0052] Quinoa peptides were included in this novel composition as a complementary natural peptide technology, because it provided unexpected results in skin pigmentation, such as reduction of melanin content and inhibition of the melanin transfer from melanocytes to keratinocytes, showing the ability to reduce skin pigmentation and enhance skin luminosity, brightening, glow and enhancing the homogenous aspect of the skin, as well as providing overall anti-aging benefits to skin.
[0053] Pea peptides and Quinoa peptides were combined at an optimal ratio with niacinamide as skin lightening active to obtain a novel composition addressing skin pigmentation unmet needs, to enhance skin glow, radiance, brightness, luminosity, lightness and to reduce dark spots/hyperpigmentation and provide an even skin tone, a uniform, homogenous and a healthy skin complexion.
[0054] There are different methods of obtaining Pea and Quinoa peptides from protein biomasses. Depending on the embodiment, pea and quinoa peptides may be prepared using hydrolysis via protease or other enzymes, such as, but not limited to, bromelain, chymotrypsin, ficin, papain, amylase, cellulase, trypsin, alkaline proteases and pepsin enzymes, and a combination of those, as well as other techniques. It is known that longer enzyme activity forms more soluble forms. It is known that hydrolysis process can be a function of temperature, time, and pH parameters. Various temperatures may also be utilized to optimize conditions. Different pH values, time, and temperatures ranges may be utilized for the controlled hydrolysis process, depending on the embodiment and may affect the peptide functional properties.
[0055] Again, there are different methods of obtaining Pea and Quinoa peptides which are then combined with a skin active, such as niacinamide. The Pea and Quinoa plant peptides processes, as noted above, utilize controlled parameters and conditions to obtain lower average MW distribution between 200-2000 Da. Furthermore, when combining the three main ingredients of the Skin Composition, Pea and Quinoa peptides and niacinamide, whether or not a preservative is present, the pH ideally should be between 4.5-6.0 to avoid any stability issues.
Biological Mechanism of Action.
[0056] This novel composition provides a multi-targeted biological mechanism of action and provides a biological effect across all skin epidermis layers, from the skin surface, stratum corneum and viable epidermis. Each component provides multi-targeted biological mechanism of action on skin and work via complementary mechanisms.
[0057] Pea peptides inhibits melanin transfer from melanocytes to keratinocytes, reduces dark spots/hyperpigmentation. Quinoa peptides inhibit melanin transfer from melanocytes to keratinocytes thus reduced skin pigmentation. Niacinamide controls melanin distribution and dispersion in the epidermis at skin surface to enhance skin lightening, brightness and even skin tone.
[0058] This unique and optimal combination of two natural peptides Pea peptides and Quinoa peptides with skin lightening active niacinamide provided unexpected results and immediately enhance skin glow, skin radiance, even skin tone, skin complexion, skin homogeneity, uniformity, reduce dark spots and hyperpigmentation, after only 7 days of treatment and providing effects after 28 days of treatment demonstrated during the in vivo clinical studies.
Description of the In Vitro and In Vivo Clinical Study Results
[0059] In Vitro study was conducted on MelanoDerm 3D in vitro Asian model (MatTek) containing normal human derived epidermal keratinocytes and melanocytes. Skin Care Composition (#SG4-136) was evaluated for its ability to decrease melanin content via spectrophotometric analysis (measurement of melanin absorbance units at 403 nm) on 3D MelanoDerm in vitro Asian skin model. Measurements were compared versus untreated skin and performed in triplicates.
[0060] In vivo clinical study was conducted on 18 human Asian female volunteers with dark spots/hyperpigmentation on the face and dark skin complexion (Fitzpatrick type IV).
[0061] Several measurements were conducted to evaluate the ability of Skin Care Composition (# SG4-136) formulated in a standard vanishing cream (formula SG4-42A) to enhance skin glow, radiance and even skin tone and to reduce dark spots/hyperpigmentation for a homogenous and more uniform skin complexion.
[0062] Formula SG4-42A cream (containing Skin Care Composition # SG 4-136) was compared to a placebo cream (SG5-42B) in this single blinded randomized half-face in vivo clinical study.
[0063] The below examples are given merely to illustrate the principles of the invention. They do not necessarily limit the scope of the invention to these or any particular embodiments.
Study 1. In Vitro B16 Melanoma Assay
[0064] The in vitro B16 melanoma cells are standard cell cultures models used herein to investigate skin depigmentation effect of biological actives and their effect on melanogenesis. In this in vitro study using B16 melanoma cells, it was proven the synergist effect between the 2 natural peptides for this skin care composition and the synergy action of all 3 complementary ingredients Pea peptides, Quinoa peptides and niacinamide when used at the recorded levels.
[0065] In the study B16 melanoma cells were tested using a Spectrophotometric method melanin absorption measured at 405 nm. The B16 melanoma cells were treated with materials for 48 hours. Again, Melanin content was measured via spectrophotometric method (at 405 nm). Measurements were conducted in triplicates. Concentrations were determined based on prescreening MTT assay which determined cell viability. Analysis was done using Student T-test comparison between groups with a (p<0.05). Samples included an Untreated sample, Skin Care Composition at 0.1% (solution), Pea peptides, Quinoa peptides and a Pea+Quinoa peptides combination at corresponding use levels used in Skin Care Composition. Concentrations were determined based on prescreening MTT cytotoxicity assay. MTT assay is an in vitro colorimetric assay for determining cell viability and cytotoxicity activity.
[0066] Study results prove that when used alone each natural peptide decrease the melanin content statically significant better compared to untreated control.
[0067] However, when Pea and Quinoa peptides are used in a specific optimal ratio, the peptide blend decreased the melanin content in vitro B16 melanoma cells statistically significant better compared to untreated and compared to each peptide alone, thus proving synergy.
[0068] When the 2 natural peptides where combined with niacinamide at an optimal ratio, the Skin Care composition (#SG4-136), a combination of three main ingredients in a specific ratio of two natural peptides, Pea peptides and Quinoa peptides, with a skin active niacinamide, described herein that includes decreased the melanin content statistically significant better compared to untreated, each peptide alone, and the combination of the 2 peptides, thus proving synergistic effect due to complementary technologies used in this skin care composition.
[0069]
Study 2. In Vitro Melanin Assay Protocol and Results
[0070] This study demonstrated that skin pigmentation on 3D skin model named RHE-MEL had a statistically significant increased individual typology angle (ITA°) brightening skin than untreated and niacinamide benchmarks after 14 days after treatment. As previously described t calculated ITA° classifies skin types into 7 physiologically different categories: very light, light, intermediate, tan, brown, dark and very dark. According to their ITA°, individuals are assigned into one of the seven skin classification categories. The Skin Care composition with the three ingredients, described above, visibly increased skin glow, radiance, brightness and even skin tone in the RHE-MEL 3d Model as shown in
[0071] The protocol for this study including the following. A melanized reconstructed human epidermis 3D Model (RHE-MEL) (in vitro Caucasian skin model) was used. The study duration was for 14 days. The RHE-MEL 3D in vitro skin tissues were allowed to grow in the first 4 days. The 3D skin tissues were treated every day with the materials for 10 days. The following samples were used: Untreated sample, Benchmark Niacinamide (at same use level as in Skin Care Composition), and Skin Care Composition used at 1%. Concentrations of materials were predetermined based on screening MTT assay. A C-Cube Dermoscope® (a high quality magnifying lens and a powerful lighting system used to evaluate pigmented skin lesions, and can diagnose melanoma), was used coupled with C-Cube clinical software for image analysis. Skin pigmentation was analyzed via high resolution pictures obtained from the C-Cube Dermoscope® coupled with an image analysis software. ITA° parameter was measured as degree of pigmentation of the skin directly on in vitro 3D RHE-MEL skin model. Statistical analysis was done using the Student T-test with a confidence level of (p<0.05).
[0072] In this 3D RHE-MEL in vitro Caucasian model skin measured was the intensity of the skin pigmentation degree by ITA° parameter (individual typological angle). Measurements were conducted for the untreated skin, skin treated with Niacinamide (used as benchmark), and skin treated with Skin Care composition after 14 days of treatment. Niacinamide increased the ITA°. The skin pigmentation degree presented significantly better as compared to untreated skin sample. Skin appears lighter, brighter but with an uneven looking appearance after Niacinamide treatment. Skin Care composition treatment to the tissue sample increased the ITA°. The skin pigmentation ITA degree of the Skin Care composition treated tissue sample presented statistically better as compared to untreated and Niacinamide treated tissue samples. The 3D RHE-MEL skin model treated with this Skin Care composition showed increased skin glow, radiance, evenness, and a more uniform and homogeneous appearance compared to untreated and niacinamide treatment.
[0073] The complementary technologies from the Skin Care composition such as Pea peptide, Quinoa peptide and Niacinamide are proven to work synergistically to improve skin glow, radiance, even skin tone, homogeneity, smoothness and increase the overall brightness and luminosity of the 3D RHE-MEL in vitro skin model.
Study 3. In Vitro Melanin Assay Protocol and Results
[0074] This composition was tested on melanoderma 3D in vitro Asian skin tissues (MatTek) containing normal human derived epidermal keratinocytes and melanocytes. Melanocytes undergo melanogenesis which leads to melanin accumulation within the tissue overtime. Skin Care composition (SG4-136) was used at 5% solution concentration. The melanoderm tissues were treated with this composition every 24 hours for 15 days. Spectrophotometric measurements of the melanin content (melanin absorbance units at 405 nm) were measured on untreated skin and on skin treated with this composition. Skin tissues were placed in 12 well plate containing 5 ml of agarose assay medium at 37° C. Test materials were applied to the surface of the skin tissues and incubated at 37C. Every 24 hours skin tissues were rinsed with PBS, fresh test material was applied, and the medium was changed every 48 hours. Treatments were carried out for 15 days. Melanin content was measured in the untreated skin tissues and skin tissues treated with Skin Care Composition (SG4-136). Measurements were performed in triplicates. Again, melanin content (mg/ml tissue) was expressed as absorbance measurements at 405 nm. Analysis of Variance (ANOVA) (One-way analysis of variance; p<0.05) was done to compare the results of this testing.
[0075]
[0076] The effect is visible as is clearly illustrated in
Study 4. In Vivo Clinical Study Protocol and Results
[0077] The in vivo clinical study was conducted on 18 Asian (Thai) female volunteers with dark spots, hyperpigmentation, uneven skin tone and dark skin complexion (Fitzpatrick type IV). This was a single blinded half-face randomized type clinical study.
[0078] Skin Care Composition Cream (SG5-42A) containing skin care composition (#SG 4-136) at 5% was tested against a placebo cream (SG5-42B) and versus baseline skin conditions for a duration of 28 days.
[0079] For this in vivo study the following instrumental measurements were taken at baseline, after 7 days and 28 days of treatment. Complexion grading was assessed on pictures (VISIA® standard light) by trained jury of 3 clinicians. Parameters measured were radiance scores, even complexion scores, skin glow index. Rating scores for these parameters were 0 (worst) to 10 (best). Radiance scores measure the visual perception by human eye of luminosity emanating from the entire skin tone on the face. Rating scale from 0 to 10 where 0 is dull, while and 10 is radiant/luminous.
[0080] Even complexion scores measure the uniform aspect of the skin on the face with no visible marks and no change in skin tone. Changes in skin tone (from dark to light) are not considered. Changes in skin redness and rosacea are considered. Rating scale from 0 to 10 where 0 means not even skin, while 10 means very even skin.
[0081] Skin glow index measures the sum between radiance scores and even complexion scores. Skin appearance is glass/porcelain like, more radiant, with an even, homogenous, and uniform skin complexion and with a glow and healthier look.
[0082] During the same in vivo study, ITA° (Individual typological angle) on dark spots/hyperpigmentation and ΔEa*b* parameters were also measured with a Spectrocolorimeter® CM 700D. Digital pictures were taken with VISIA® instrument at baseline, after 7 days and 28 days of treatment with both products.
[0083] ITA° (Individual Typological angle) on dark spots measures the skin pigmentation degree of a subject by measuring L* (luminosity) and b* (cutaneous melanin) components. The higher the ITA°, the lighter and more luminous spots and less hyperpigmentation. An increase in ITA° compared to baseline it means the dark spots are less visible, lighter, and more luminous.
[0084] The formula expressed as ΔEa*b* (dark spots−normal skin) measures changes of the skin hue and luminosity. A decrease in ΔEa*b* compared to baseline means an attenuation of pigmentation differences between normal skin and dark spots. This means that dark spots are less visible and less perceivable compared to the normal skin background in near vicinity of the spot, thus skin is more homogenous, uniform with an even tone and an even complexion.
[0085] Volunteers were also asked to provide subjective evaluations after 28 days of treatment and to evaluate both products for several sensorial parameters versus baseline and compared to each other.
[0086] The in vivo clinical studies conducted on Asian skin female subjects with dark spots, hyperpigmentation, uneven skin tone and dark skin complexion, demonstrated that this skin care composition cream SG5-42A which contains skin care composition (# SG4-136) enhances skin glow, radiance, reduces dark spots/hyperpigmentation, providing an evened skin tone, a homogenous and more uniform skin appearance compared to baseline and placebo, after only 7 days and 28 days of treatment. The in vivo clinical study results, drawings, graphs, and digital pictures are illustrated in
[0087]
[0088]
[0089]
[0090]
[0091] Based on in vivo clinical study results SG5-42A cream increased skin radiance scores and skin glow index after only 7 and 28 days and performed statistically significant better compared to baseline and placebo cream. SG5-42A cream also increased ITA° thus decreased hyperpigmentation and dark spots for less visible lighter spots and an even tone after only 7 and 28 days compared to baseline and placebo cream.
[0092] The results are statistically significant better compared to baseline after 7 days and 28 days of treatment.
[0093] In Vivo clinical study results demonstrate that formula SG5-42A cream decreased ΔEa*b* parameter, thus skin pigmentation and hue (difference between dark spots and normal skin) for more uniform, homogenous and even skin tone after only 7 and 28 days. The results are statistically significant better compared to baseline and placebo after 7 days and 28 days of treatment.
[0094] During subjective evaluations conducted on Asian female volunteers were asked to provide subjective evaluations after 28 days of treatment and to evaluate both products for several sensorial parameters versus baseline and compared to each other.
[0095]
[0096] Subjective evaluation study results demonstrated that after 28 days of treatment with SG5-42A cream versus baseline.
[0097] These results indicated the following: [0098] 89% of subjects felt that their skin was glower [0099] 83% of subjects felt that their skin was more radiant [0100] 78% of subjects perceived even skin complexion [0101] 78% of subjects perceived that dark spots and blemishes are reduced [0102] 56% of subjects perceived a glass/porcelain appearance of the skin
[0103] SG5-42A cream was perceived by all subjects to significantly increase the skin glow, radiance, even skin complexion and to reduce dark spots for a glass/porcelain skin appearance compared to baseline after 28 days of treatment.
[0104] The sensory performance of product SG5-42A was perceived to perform better than placebo after 28 days of treatment.
[0105]
[0106] After 7 days and 28 days of treatment with SG5-42A cream skin looks radiant, brighter, glower, lighter while dark spots/hyperpigmentation visibly diminished and skin tone is more even, uniform, and homogenous compared to baseline and placebo skin sites.
[0107]
In Vivo Clinical Study Summary of Results
[0108] Based on in vivo clinical study results SG5-42A cream increased skin radiance scores and skin glow index after only 7 and 28 days and performed statistically significant better compared to baseline and placebo cream.
[0109] SG5-42A cream also increased ITA° thus decreased hyperpigmentation and dark spots for less visible lighter spots and an even tone after only 7 and 28 days compared to baseline and placebo cream. The results are statistically significant better compared to baseline after 7 days and 28 days of treatment.
[0110] In Vivo clinical study results demonstrate that formula SG5-42A cream decreased ΔEa*b* parameter, thus skin pigmentation and hue (difference between dark spots and normal skin) for more uniform, homogenous and even skin tone after only 7 and 28 days.
[0111] The results are statistically significant better compared to baseline and placebo after 7 days and 28 days of treatment.
[0112] SG5-42A cream was perceived by all subjects to significantly increase the skin glow, radiance, even skin complexion and to reduce dark spots for a glass/porcelain skin appearance compared to baseline after 28 days of treatment.
[0113] The sensory performance of product SG5-42A was perceived to perform better than placebo after 28 days of treatment.
[0114] The results are visible and clearly demonstrated via digital pictures for a representative subject (age 51 years old).
[0115] This Asian volunteer (51 years old) showing at Day 0 (baseline) dark spots, hyperpigmentation, and uneven skin tone.
[0116] After only 7 days and 28 days of treatment with SG5-42A cream skin looks radiant, brighter, glower, lighter while dark spots/hyperpigmentation visibly diminished and skin tone is more even, uniform, and homogenous compared to baseline and placebo skin sites.
TABLE-US-00002 TABLE II Formulation Composition # SG5-42A (with Skin Care Composition SG4- 136) and SG5-42B (placebo) used for the in vivo clinical study Percentage (%) SG5-42A Example (With Skin Percentage Commercial Example Care (%) SG5-42B Ingredient Name Name Supplier Composition) (Placebo) Phase A Water Water 66.456 71.456 Glycerin Glycerin 99.5% Protameen 1.00 1.00 Natural USP Chemicals Tetrasodium Glutamate Diacetate BioPure ™ GLDA Jarchem 0.04 0.04 Industries, Inc. Phase B Stearic Acid Stearic Acid TP Jeen Intl. 17.90 17.90 NF Corp. Cetyl Alcohol Cetyl Alcohol NF Jeen Intl. 0.53 0.53 Corp. Hydrogenated Ethylhexyl Fision ® EcoSil TRI-K 0.50 0.50 Olivate, Hydrogenated Industries, Olive Oil Unsaponifiables Inc. Isopropyl Myristate Jeechol IPM Jeen Intl. 0.75 0.75 Corp. Titanium Dioxide, Aluminum TRIShield T-110 TRI-K 0.20 0.20 Hydroxide, Stearic Acid Industries, Inc. Tocopheryl Acetate Vitamin E TRI-K 0.10 0.10 Acetate Industries, Inc. Ethylhexyl Methoxycinnamate Galaxy 2932 Galaxy 0.75 0.75 Surfactants Ltd. Butyl Methoxydibenzoylmethane Jeescreen A Jeen Intl. 0.40 0.40 Corp. Phase C Water Water 5.00 5.00 Potassium Hydroxide Potassium J. T. Baker 0.484 0.484 Hydroxide, Pellets Phase D Water, Niacinamide, Pea Skin Care TRI-K 5.00 0.00 Peptides, Quinoa peptides Composition Industries, (#SG4-136) Inc. Phase E Phenoxyethanol TRIstat P5 TRI-K 0.89 0.89 Industries, Inc.
Procedure:
[0117] 1) Heat water to 85° C. and add Phase A ingredients one at a time until dissolve and uniform.
[0118] 2) Combine Phase B with heat and mixing to 85° C. Combine Phase C.
[0119] 3) Transfer Phase B to Phase A to emulsify and switch to homogenizer.
[0120] 4) Do not let temperature drop. Keep at 85° C. Add Phase C under homogenizer.
[0121] 5) Switch to side sweep mixing. Cool to 40° C. Add Phase D and Phase E.
Skin Care Composition: Summary of Skin Benefits and Claims
[0122] This novel composition contains two natural peptides Pea and Quinoa, which work via complementary biological mechanism of action in skin, and are combined at an optimal ratio with the skin lightening active Vitamin B3 (Niacinamide). This optimal combination of Vitamin B3 (Niacinamide) and complementary natural Pea and Quinoa peptides can offer a holistic approach to the skin glow through multi targeted action controlling key biological pathways to deliver the desired end benefit of enhancing skin glow, skin radiance and even skin tone and even skin complexion.
[0123] This unique and optimal combination of two natural peptides Pea and Quinoa with the skin lightening active niacinamide provided unexpected results and immediately enhance skin glow, skin radiance, even skin tone, skin complexion, skin homogeneity, uniformity, reduce dark spots and hyperpigmentation, after only 7 days of treatment and providing effects after 28 days of treatment demonstrated during the in vivo clinical studies.
[0124] This novel composition provides a multi-targeted biological mechanism of action and provides a biological effect across all skin epidermis layers, from the skin surface, stratum corneum and viable epidermis. Each component provides multi-targeted biological mechanism of action on skin and work via complementary mechanisms. Pea peptides inhibit melanin transfer from melanocytes to keratinocytes, reduces dark spots/hyperpigmentation. Pea peptides inhibit melanin transfer from melanocytes to keratinocytes, reduces dark spots/hyperpigmentation. Quinoa peptides inhibit melanin transfer from melanocytes to keratinocytes thus reduced skin pigmentation. Niacinamide controls melanin distribution and dispersion in the epidermis at skin surface to enhance skin lightening, brightness and even skin tone.
Summary of Results
[0125] Below is a brief summary of the results using this novel composition. Again these embodiments are given to merely illustrate the principles of the invention and are not meant to limit the invention to these particular examples.
[0126] Pea peptides inhibit melanin transfer from melanocytes to keratinocytes, reduces dark spots/hyperpigmentation. Quinoa peptides inhibit melanin transfer from melanocytes to keratinocytes thus reduce skin pigmentation. Niacinamide controls melanin distribution and dispersion in the epidermis at skin surface to enhance skin lightening, brightness and even skin tone.
[0127] Clinically proven to enhance skin glow and radiance after only 7 and 28 days of treatment.
[0128] Clinically proven to enhance even skin tone, homogeneity, and uniformity of the skin complexion after only 7 days and 28 days of treatment.
[0129] Clinically proven to minimize dark spots/hyperpigmentation and to provide an even skin tone and even complexion in only 7 & 28 days of treatment.
[0130] Subjective evaluations results proved that Asian female subjects perceived their skin was glower, more radiant with an even skin tone, reduced dark spots and blemishes, the appearance of skin was glass/porcelain looking like after 28 days of treatment with SG5-42A cream compared to baseline and placebo treatment.
[0131] Digital pictures demonstrate visible improvements for a representative Asian volunteer (51 years old) which shows at Day 0 (baseline) dark spots, hyperpigmentation, and uneven skin tone. After 7 days and 28 days of treatment with SG5-42A cream skin looks more radiant, brighter, glower, while dark spots/hyperpigmentation are visibly diminished, while skin tone is more even, uniform, and homogenous compared to baseline and placebo skin sites.
[0132] Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of the principles and applications of the present invention. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as defined by the appended claims.