Multichannel Close-up Imaging Device

Abstract

A device for optically imaging at least a part of an object, the device having an optical axis and including a two-dimensional first array of first microlenses, having a first side intended to face the object, and a second side, opposite the first side, a two-dimensional second array of second microlenses, each first microlens being aligned with a second microlens on an axis parallel to the optical axis, wherein each first microlens comprises a first catoptric system, and preferably a first catadioptric system.

Claims

1-13. (canceled)

14. A device for optically close-up imaging at least a part of an object, the device having an optical axis and comprising: a first array of catoptric systems or catadioptric systems, the first array comprising: a first slide of transparent material; a second slide of transparent material disposed adjacent the first slide of transparent material; a first plurality of mirrors disposed on the first slide; and a second plurality of mirrors disposed on the second slide, wherein: each of the first plurality of mirrors is configured to reflect a light ray directed toward the first slide by the second plurality of mirrors, each mirror of the second plurality of mirrors comprises a hole, the hole configured to transmit the light ray reflected by the first plurality of mirrors therethrough, and each catoptric system or catadioptric system of the first array comprises a mirror of the first plurality of mirrors and a mirror of the second plurality of mirrors; and a second array of catoptric systems or catadioptric systems, the second array comprising: a third slide of transparent material disposed adjacent the second slide such that the second slide is disposed between the first slide and the third slide; a fourth slide of transparent material disposed adjacent the third slide of transparent material such that the third slide is disposed between the second slide and the fourth slide; a third plurality of mirrors disposed on the third slide; and a fourth plurality of mirrors disposed on the fourth slide, wherein: each mirror of the third plurality of mirrors comprises a hole, the hole configured to transmit the light ray reflected by the first plurality of mirrors therethrough, each mirror of the fourth plurality of mirrors is configured to reflect the light ray from the third plurality of mirrors; and each catoptric system or catadioptric system of the second array comprises a mirror of the third plurality of mirrors and a mirror of the fourth plurality of mirror.

15. The device of claim 14, wherein each of the mirrors of the first plurality of mirrors, the second plurality of mirrors, the third plurality of mirrors, and the fourth plurality of mirrors is a thin film mirror.

16. The device of claim 14, wherein each of the catoptric systems or the catadioptric systems of the first array has a magnification less than 0.2.

17. The device of claim 14, wherein each of the catoptric systems or the catadioptric systems of the first array is configured for having a predetermined field of view, each of the catoptric systems or the catadioptric systems of the first array forming a first optical channel having a diameter D.sub.ch1, a size of the predetermined field of view being greater than the diameter D.sub.ch1 of the first optical channel.

18. The device of claim 17, wherein the size of the predetermined field of view is 5 times greater than the diameter D.sub.ch1 of the first optical channel so that multiple optical channels are adapted for imaging the same object point.

19. The device of claim 14, wherein a catoptric system or catadioptric system of the first array is aligned with a catoptric system or catadioptric system of the second array to form a unitary optical system, the unitary optical system being symmetrical relative to a plane extending perpendicular to the optical axis and extending between the second slide and the third slide.

20. The device of claim 14, wherein: each mirror of the second plurality of mirrors is disposed on a surface of the second slide that faces the third slide, and each mirror of the first plurality of mirrors is disposed on a surface of the first slide that faces the second slide.

21. The device of claim 14, wherein: each mirror of the second plurality of mirrors is disposed on a surface of the second slide facing the first slide, and each mirror of the first plurality of mirrors is disposed a surface of the first slide facing the second slide.

22. The device of claim 14, wherein each mirror of the plurality of second mirrors is concave and each mirror of the plurality of first mirrors is convex.

23. The device of claim 14, wherein each mirror of the plurality of first mirrors and the plurality of second mirrors is concave.

24. The device of claim 14, further comprising a dioptric microlens arranged between a mirror of the first plurality of mirrors and a mirror of the second plurality of mirrors.

25. The device of claim 24, wherein the dioptric microlens is covers a surface of a mirror of the first plurality of mirrors.

26. The device of claim 14, further comprising a positive dioptric microlens arranged in the hole of the mirrors of the first plurality of mirrors.

27. The method of using the device of claim 14, comprising: emitting an isotropic light from an object; and reflecting the isotropic light through the device of claim 14 using the first array of catoptric systems or catadioptric systems and the second array of catoptric systems or catadioptric systems to obtain an image of the object.

28. The method of claim 27, wherein the object is a fluorescent object.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0037] The invention will be described by way of example, with reference to the accompanying drawings in which:

[0038] FIG. 1 diagrammatically illustrates a single optical channel of the prior art,

[0039] FIG. 2 diagrammatically illustrates another multichannel close-up optical device from the prior art wherein each lens array comprises a transparent substrate,

[0040] FIG. 3 diagrammatically illustrates a multichannel close-up imaging device from the prior art comprising diaphragms,

[0041] FIG. 4 diagrammatically illustrates an optical device according to an embodiment of the invention comprising an array of catoptric first lenses,

[0042] FIG. 5 diagrammatically illustrates an optical device according to an embodiment of the invention comprising two lenses, each lens comprising a Cassegrain combination of two reflectors,

[0043] FIG. 6 diagrammatically illustrates an optical device according to an embodiment of the invention comprising two lenses, each lens comprising another Cassegrain combination of two reflectors,

[0044] FIG. 7 diagrammatically illustrates an optical device according to an embodiment of the invention comprising two lenses, each lens comprising a Gregorian combination of reflectors,

[0045] FIG. 8 diagrammatically illustrates an optical device according to an embodiment of the invention comprising two lenses, each lens comprising a Newtonian combination of reflectors and dioptric lens,

[0046] FIG. 9 diagrammatically illustrates an optical device according to an embodiment of the invention comprising two lenses, each lens comprising another Cassegrain combination of reflectors,

[0047] FIG. 10 diagrammatically illustrates an optical device according to an embodiment of the invention comprising two lenses, one of the lenses comprising another Cassegrain combination of reflectors,

[0048] FIG. 11 diagrammatically illustrates an optical device according to an embodiment of the invention comprising two lenses, each lens comprising a Gregorian combination of reflectors, and

[0049] FIG. 12 diagrammatically illustrates an optical device according to an embodiment of the invention comprising two lenses, one of the lenses comprising another Cassegrain combination of reflectors.

DEFINITIONS

[0050] The term “catoptric system” will be used herein to designate an optical system comprising at least two mirrors adapted to form an image of an object.

[0051] The term “catadioptric system” will be used herein to designate an optical system comprising at least two mirrors in combination with a refraction-based dioptric lens, said catadioptric system being adapted to form an image of an object.

[0052] The term “lens” will be used herein to designate an optical system adapted to focus or to disperse a light ray by means of refraction (using a dioptric system), or reflection (using a catoptric system), or both.

[0053] The term “convex” will be used herein to designate an object having a surface which has a protruding curvature relative to an incident light ray. Consequently, the terms “convex mirror” herein designate a mirror adapted to diverge a light ray, while the terms “convex dioptric lens” herein designate a dioptric lens adapted to converge a light ray.

[0054] The term “concave” will be used herein to designate an object having a surface which has a grooving curvature relative to an incident light ray. Consequently, the terms “concave mirror” herein designate a mirror adapted to converge a light ray, while the terms “concave dioptric lens” herein designate a dioptric lens adapted to diverge a light ray.

[0055] The term “positive” lens, respectively “negative” lens, will be used herein to designate a lens adapted to converge an incident light ray, respectively to diverge an incident light ray.

[0056] The term “microlens” will be used herein to designate a lens having a diameter lower than 2 mm, notably lower than 500 μm, and preferably lower than 300 μm.

[0057] The term “transparent” will be used herein to designate a material absorbing less than 50% of the energy of light rays in the visible range, preferably without changing the wavelength of said rays.

DETAILED DESCRIPTION

[0058] Referring to FIG. 4, the optical device 1 according to an embodiment is adapted to image at least a part of an object 2. The device 1 may be adapted for close-up imaging. Close-up imaging refers to imaging of an object arranged at a close distance from the optical device 1, such as a distance that is less than 2 cm from the optical device 1 or less than 1 cm.

[0059] The device 1 has an optical axis 3 along which different components of the optical device are aligned. The device 1 comprises at least a two-dimensional first array 4 of first microlenses 5. The two-dimensional first array 4 may form a plane perpendicular to the optical axis 3, each of the first microlenses 5 being aligned on an axis parallel to the optical axis 3. The first array 4 has a first side intended to face the object 2, and a second side opposite the first side.

[0060] Each of the first array 4 comprises at least a first catoptric system 8, and preferably a first catadioptric system. The first catoptric system comprises preferably at least two reflective components: a primary mirror 14 and a secondary mirror 12. Each mirror (primary mirror 15 or secondary mirror 12) has an optical axis aligned with the optical axis 3.

[0061] Each first microlens 5 defines a first optical channel 24, coming from the object 2 to the first microlens 5. The first optical channel 24 has a diameter D.sub.ch1. Each first microlens 5 also forms a second optical channel 25 from the side of the first microlens 5 opposite the object 2 toward a direction opposite the object 2. The second optical channel 25 has a diameter D.sub.ch2. The first catoptric system allows forming a second optical channel 25 having a diameter D.sub.ch2 inferior to the diameter D.sub.ch1, preferably smaller than 0.3 times D.sub.ch1. These conditions between diameters of the optical channels allow for avoiding crosstalk between the different adjacent optical channels of adjacent first microlenses 5. Moreover, the first catoptric system allows for a shortened focal length of the first microlens 5 compared with a pure dioptric microlens 5. Hence, it is possible to form an intermediate image from the first microlens 5 smaller than an intermediate image formed by a pure dioptric microlens. Finally, the first microlens 5 comprising the first catoptric system allows for increasing the field of view of the optical device 1 compared with an optical device 1 comprising an array of pure dioptric lenses facing the object 2. The shortened focal length and increased field of view allows for increasing the number of first catoptric systems and/or catadioptric system adapted for imaging a single object point, and then increasing the resolution of an image while avoiding ghost image resulting from crosstalk. The first catoptric system and/or the first catadioptric system have preferably a magnification less than 0.2, and preferably less than 0.1, so that the total resolution of the imaging stem can be increased.

[0062] In a preferred embodiment of the present disclosure, the first catoptric system and/or the first catadioptric system is configured for having field of view. A dimension of the field of view is greater than the diameter D.sub.ch1 of the first optical channel, and may be 5 times greater than the diameter D.sub.ch1 of the first optical channel. Multiple optical channels are then allowed for imaging the same object point.

[0063] Another aspect of the present disclosure is the use of the device 1 for imaging an object 2 emitting an isotropic light, and preferably for imaging a fluorescent object. The fluorescent object can be, for example, a fluorescent dye or a fluorophore. Because the optical device 1 allows for imaging the object 2 with a wider field of view compared with optical devices of the prior art, the use of the device 1 is particularly adapted for imaging an object emitting an isotropic light. Preferably, the device 1 is used for imaging a set of fluorescent dyes arranged in the object plane of the device.

[0064] The optical device 1 comprises a two-dimensional array 6 of second microlenses 7. The two-dimensional second array 6 may form a plane perpendicular to the optical axis 3, each of the second microlenses 7 being aligned on an axis parallel to the optical axis 3. Each second microlens 7 is aligned with a first microlens 5 on an axis parallel to the optical axis 3, forming a unitary optical system comprising the first microlens 5 and the second microlens 7. Each second microlens 7 can be chosen between at least a pure dioptric microlens and a catoptric microlens, preferably a catadioptric microlens.

[0065] Referring to FIG. 5, the first array 4 can comprise a first slide 11 of transparent material arranged to face the object 2. The first slide 11 can be for example a glass slide or a glass wafer.

[0066] The first array 4 can further comprise a first two-dimensional array of thin film secondary mirrors 12, each secondary mirror 12 covering the first slide 11. The two-dimensional array of thin film secondary mirrors 12 may form a plane perpendicular to the optical axis 3.

[0067] Each secondary mirror 12 is adapted to reflect at least a light ray coming from a side opposite the object 2 relative to the first slide 11. The mirrors of the different embodiments of the invention can be fabricated by standard microlens fabrication and deposition processes, comprising for example melting resist technology, imprint technology, and/or deposition processes. An array of secondary mirrors 12 can be fabricated for example by lift-off deposition of a thin reflective material layer, preferably a thin metal layer. The metal used for the fabrication of the mirrors may be selected from aluminium, silver, and/or gold.

[0068] The first array 4 can further comprise a second slide 13 of transparent material, the first slide 11 being arranged between the object 2 and the second slide 13. A second two-dimensional array of thin film primary mirrors 14 covers the second slide 13. Each primary mirror 14 is adapted to reflect a light ray coming from a side facing the object 2 relative to the second slide 13. Each primary mirror 14 comprises a hole 15, said hole 15 being adapted to transmit light reflected by the secondary mirror 12 through the primary mirror 14. The hole 15 may be fabricated in the center of the primary mirror 14.

[0069] The first slide 11, the first array of secondary mirrors 12, the second slide 13 and the second array of secondary mirrors 14 allow to fabricated the two-dimensional first array 4 of first microlenses 5, each first microlens 5 comprising a catoptric system. The catoptric system comprises the primary mirror 14 and the secondary mirror 12. Referring to FIG. 5, each first microlens 5 can then be designed as a Cassegrain reflector.

[0070] The optical device 1 can also comprise a third slide 16 of transparent material arranged on a side opposite to the object 2 relative to the first array 4. The third slide 16 can be for example a glass slide or a glass wafer.

[0071] A third two-dimensional array of thin film quaternary mirrors 17 can cover the third slide 16. The two-dimensional array of thin film quaternary mirrors 17 may form a plane perpendicular to the optical axis 3. Each quaternary mirror 17 is adapted to reflect at least a light ray coming from a side opposite the object 2 relative to the third slide 16.

[0072] The optical device 1 can further comprise a fourth slide 18 of transparent material, the third slide 16 being arranged between the first array 4 and the fourth slide 18. A fourth two-dimensional array of thin film tertiary mirrors 19 covers the fourth slide. Each tertiary mirror 19 is adapted to reflect a light ray coming from a side facing the object 2 relative to the fourth slide 18. Each tertiary mirror 17 comprises a hole 15, said hole 15 being adapted to transmit light reflected by the secondary mirror 12 through the primary mirror 14.

[0073] The third slide 16, the third array of quaternary mirrors 17, the fourth slide 18 and the fourth array of tertiary mirrors 17 allow to fabricate the two-dimensional second array 6 of second microlenses 7, each second microlens 7 comprising a second catoptric system. The second catoptric system comprises the tertiary mirror 19 and the quaternary mirror 17. In reference to FIG. 5, each second microlens 7 can be then designed as a Cassegrain reflector. The magnification of the second catoptric and/or catadioptric system may be higher than 5 and preferably higher than 10. Having a second catoptric system and/or a second catadioptric system instead of a pure refractive second lens allows for increasing the resolution of the image obtained by the device, by allowing having a first catoptric and/or catadioptric system with a low magnification. The increase in resolution can be obtained by avoiding ghost images resulting from crosstalk. It consequently also allows having a plurality of optical channels participating in the imaging of one object point, preferably more than 4 and notably more than 9 optical channels. It also allows to increase the global numerical aperture of the device, the global numerical aperture being composed by the segmented assembly of individual numerical apertures of each optical channel, while minimizing the formation of ghost images.

[0074] In an embodiment of the present disclosure, the first microlens 5 and the second microlens 7 of the unitary optical system are symmetrical relative to a plane 10 perpendicular to the optical axis 3. Because of the symmetry between the catoptric first microlens 5 and the catoptric second microlens 7, it is possible to remove comatic aberrations of the optical device 1, like coma and distortion.

[0075] The mirrors (e.g., the primary mirror 14 and/or the secondary mirror 12 and/or the tertiary mirror 19 and/or the quaternary mirror 17) can have a reflective surface forming a spherical surface or an aspherical surface. A mirror having a reflective surface forming an aspherical surface allows to mitigate, preferably to avoid, spherical aberration.

[0076] Each primary mirror 14 can cover a surface of the second slide opposite to the object 2 relative to the second slide 13, and each secondary mirror 12 can cover a surface of the first slide opposite to the object 2 relative to the first slide 11.

[0077] The first microlenses 5 can be mounted in a Cassegrain design: the primary mirrors 14 of the first array 4 can be concave and the secondary mirrors 12 of the first array 4 can be convex.

[0078] In reference to FIG. 6, each primary mirror 14 can cover a surface of the second slide 13 facing the object 2, and each secondary mirror 12 can cover a surface of the first slide 11 opposite to the object 2 relative to the first slide 11.

[0079] In reference to FIG. 7, both the primary mirrors 14 and the secondary mirrors 12 can be concave. Then, each first microlens 5 comprises a catoptric system mounted in a Gregorian design. A Gregorian design allows an intermediate image plane of the device 1 to be shifted away from the object 2 along the optical axis 3 compared to a Cassegrain design.

[0080] In reference to FIG. 8, each first microlens 5 can comprise a dioptric microlens, preferably a pure dioptric microlens arranged between the primary mirror 14 and the secondary mirror 12. Each first microlens 5 can be mounted in a Newtonian design. The primary mirrors 14 can be concave and the secondary mirrors 12 can be flat. The secondary mirrors 12 cover the side of the first slide 11 opposite the object 2 relative to the first slide 11, and a positive pure dioptric microlens covers each secondary mirror 12.

[0081] In reference to FIG. 9, each of the first microlenses 5 can be mounted in a Cassegrain catadioptric design. The primary mirrors 14 can be concave and the secondary mirrors 12 can be convex. The secondary mirrors 12 cover the side of the first slide 11 opposite the object 2 relative to the first slide 11, and a negative pure dioptric microlens covers each secondary mirror 12. This configuration allows the intermediate image plane 10 to be shifted away from the object 2 along the optical axis 3, and to reduce the obturation ratio compared to other Cassegrain configurations.

[0082] In reference to FIG. 10, each second microlens 7 can be a dioptric microlens, preferably comprising a third slide 16 of transparent material and a positive microlens covering a side of the third slide 16 facing the object 2 relative to the third slide 16. For example, the first microlens 5 can be mounted in a Cassegrain design, i.e. the primary mirrors 14 of the first array 4 can be concave and the secondary mirrors 12 of the first array 4 can be convex. The second microlenses 7 can be pure dioptric positive microlenses. Preferably, each second microlens 7 can be a collimator assembly of pure dioptric microlenses.

[0083] In reference to FIG. 11, the first microlenses 5 can be mounted in a Gregorian design, the primary mirrors being concave, the secondary mirrors being also concave and each secondary mirror being covered by a positive pure dioptric microlens, on the side opposite the object 2 relative to the first slide 11. This configuration allows for the intermediate image plane 10 to be shifted away from the object plane 2 along the optical axis 3, and to reduce the obturation ratio compared to Cassegrain configurations.

[0084] In reference to FIG. 12, each second microlens 7 can comprise a positive dioptric microlens arranged in the hole 15 of the primary mirror 14 of the first array 4. The first microlenses 5 can for example be mounted in a Cassegrain design, the first mirrors 14 being concave, the second mirrors 12 being convex, and the second microlenses 7 comprising a positive pure dioptric microlens inserted in the hole 15.

[0085] In any embodiment of the present disclosure, each unitary optical system can comprise both a positive microlens and a micronegative lens, so as to minimize or to avoid the Petzval effect. This allows the imaging device 1 to form a flat-field image of the object 2.