N-BENZYL-N-ARYLSULFONAMIDE DERIVATIVE AND PREPARATION AND USE THEREOF

Abstract

The invention provides an N-benzyl-N-arylsulfonamide derivative, which is an N-benzyl-N-arylsulfonamide compound represented by formula (I) or a pharmaceutically acceptable salt or solvate thereof. The N-benzyl-N-arylsulfonamide derivative is obtained by condensing a substituted nitrobenzene with 5- or 6-membered nitrogen-containing aliphatic heterocycle (the ring B), reducing the nitro group to an amino group, and subjecting the amino group to reductive amination, sulfonamidation; or by subjecting a substituted nitrobenzene to nitro reduction, reductive amination and sulfonamidation, and condensing the resultant intermediate with 5- or 6-membered nitrogen-containing aliphatic heterocycle (the ring B). It has been experimentally demonstrated that the N-benzyl-N-arylsulfonamide derivative of the invention can specifically bind to Kv1.3 potassium channel and inhibit or decrease its activity, and is useful in the treatment of autoimmune diseases caused by abnormal activation of the Kv1.3 potassium channel in human or animals. The invention further provides a medicament or a pharmaceutical composition comprising the N-benzyl-N-arylsulfonamide derivative.

##STR00001##

Claims

1. An N-benzyl-N-arylsulfonamide derivative, characterized in that it is an N-benzyl-N-arylsulfonamide compound represented by formula (I) or a pharmaceutically acceptable salt or solvate thereof ##STR00076## wherein: ring A is substituted phenyl with the substituent group being selected from the group consisting of ##STR00077## and R.sub.3 group; ring B is selected from the group consisting of 5- or 6-membered substituted or unsubstituted aliphatic heterocyclyl containing 1-3 heteroatoms selected from the group consisting of O, N and S, with the substituent group being selected from the group consisting of Z and R.sub.4; R is selected from the group consisting of C1-6 linear or branched alkyl, or halogenated C1-6 linear or branched alkyl; R.sub.1 is selected from the group consisting of substituted or unsubstituted phenyl, or 5- or 6-membered substituted or unsubstituted heteroaryl containing 1-3 heteroatoms selected from the group consisting of O, N and S, the substitution being mono-, di- or tri-substitution with the substituent group being Rb group; Rb is selected from the group consisting of H, halogen, nitro, cyano, C1-3 alkyl, C1-3 alkoxy, halogenated C1-3 alkyl and —C—O—C—; R.sub.2 is selected from the group consisting of H, C1-3 liner or branched alkyl, cyclopropyl and ═O; R.sub.3 is selected from the group consisting of halogen, nitro, cyano, trifluoromethyl, amido, substituted alkylamido; R.sub.4 is selected from the group consisting of H, halogen, ═O, OH, NH.sub.2, formate group, carbamate group, alkylacyl, acetate group, sulfonamido, pyrrolidonyl, cyclopropyl, aminoformamido, dimethylaminoethoxy, alkanoyloxy and alkylamido; Z is selected from the group consisting of O, S and NH, or is absent.

2. The N-benzyl-N-arylsulfonamide derivative according to claim 1, characterized in that the ring A is selected from the group consisting of the following substituted benzene rings: ##STR00078##

3. The N-benzyl-N-arylsulfonamide derivative according to claim 1, characterized in that the ring B is selected from the group consisting of the following 5- or 6-membered aliphatic heterocyclyl containing oxygen and/or nitrogen: ##STR00079##

4. The N-benzyl-N-arylsulfonamide derivative according to claim 1, characterized in that the N-benzyl-N-arylsulfonamide derivative is selected from the group consisting of the following compounds: ethyl 4-(2-cyano-4-((4-fluoro-N-propylphenyl)sulfonamido)phenyl) piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(4-fluorobenzyl)propanesulfonamido)phenyl) piperazin-1-formate, ethyl 4-(2-cyano-4-(N-isobutylphenylsulfonamido)phenyl)piperazin-1-formate, N-(3-cyano-4-(4-hydroxypiperidin-1-yl)phenyl)-N-(4-fluorobenzyl) propane-1-sulfonamide, 4-(2-cyano-4-(N-(4-fluorobenzyl)propyl)phenyl)-N,N-dimethyl piperazin-1-formamide, ethyl 4-(4-(N-benzylpropanesulfonamido)2-cyanophenyl)piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(3-fluorobenzyl)propanesulfonamido)phenyl) piperazin-1-formate, ethyl 4-(4-(4-(N-(4-chlorobenzyl)propanesulfonamido)2-cyanophenyl) piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(2-fluorobenzyl)propanesulfonamido)phenyl) piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(3-methoxybenzyl)propanesulfonamido)phenyl) piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(4-methoxybenzyl)propanesulfonamido)phenyl) piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(3,4-dimethoxybenzyl)propanesulfonamido)phenyl)piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(2-fluoro-4-methoxybenzyl) propanesulfonamido)phenyl)piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(4-fluoro-2-methoxybenzyl) propanesulfonamido)phenyl)piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(pyrimidin-4-ylmethyl)propanesulfonamido) phenyl)piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(pyridin-2-ylmethyl)propanesulfonamido) phenyl)piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(furan-2-ylmethyl)propanesulfonamido)phenyl) piperazin-1-formate, ethyl 4-(2-cyano-4-(N-(thiazol-4-ylmethyl)propanesulfonamido) phenyl)piperazin-1-formate, ethyl 4-(2-cyano-4-(N-((1-methyl-1H-imidazol-5-yl)methyl) propanesulfonamido)phenyl)piperazin-1-formate, ethyl 4-(2-cyano-4-(N-((1-methyl-1H-pyrazol-5-yl)methyl) propanesulfonamido)phenyl)piperazin-1-formate, 4-(2-cyano-4-((N-(4-fluorobenzyl)-2-methylpropyl)sulfonamido) phenyl)-N,N-dimethylpiperazin-1-formamide, 4-(2-cyano-4-(N-(4-fluorobenzyl)butanesulfonamido)phenyl)-N,N-dimethylpiperazin-1-formamide, 4-(2-cyano-4-((N-(4-fluorobenzyl)-3-methylbutyl)sulfonamido)phenyl)-N,N-dimethylpiperazin-1-formamide, 4-(2-cyano-4-((N-(4-fluorobenzyl)-2,2-dimethylpropyl)sulfonamido) phenyl)-N,N-dimethylpiperazin-1-formamide, 4-(2-cyano-4-(N-(pyridin-4-ylmethyl)propanesulfonamido)phenyl)-N,N-dimethylpiperazin-1-formamide, 4-(2-cyano-4-(N-(pyrimidin-4-ylmethyl)propanesulfonamido)phenyl)-N,N-dimethylpiperazin-1-formamide, 4-(2-cyano-4-(N-(4-fluorobenzyl)ethanesulfonamido)phenyl)-N,N-dimethylpiperazin-1-sulfonamide, 4-(4-(N-(4-chlorobenzyl)propanesulfonamido)-2-cyanophenyl)-N,N-dimethylpiperazin-1-sulfonamide, 4-(2-cyano-4-(N-(4-methoxybenzyl)propanesulfonamido)phenyl)-N,N-dimethylpiperazin-1-sulfonamide, 4-(2-cyano-4-((1-cyclopropyl-N-(2,4-difluorobenzyl)methyl) sulfonamido)phenyl)-N,N-dimethylpiperazin-1-sulfonamide, ethyl 4-(4-(N-(4-fluorophenyl)propanesulfonamido)-2-(trifluoro methyl)phenyl)piperazin-1-formate, N-(3-cyano-4-(piperidin-4-yloxy)phenyl)-N-(4-fluorobenzyl)propane sulfonamide, N-(3-cyano-4-(piperidin-4-ylthio)phenyl)-N-(4-fluorobenzyl)propane sulfonamide, N-(3-cyano-4-(piperidin-4-ylamino)phenyl)-N-(4-fluorobenzyl) propanesulfonamide, N-(4-((1-acetylpiperidin-4-yl)oxy)-3-cyanophenyl)-N-(4-fluorobenzyl) propanesulfonamide, N-(4-((1-acetylpiperidin-4-yl)thio)-3-cyanophenyl)-N-(4-fluorobenzyl) propanesulfonamide, N-(4-((1-acetylpiperidin-4-yl)amino)-3-cyanophenyl)-N-(4-fluoro benzyl)propanesulfonamide, N-(4-((1-formylpiperidin-4-yl)oxy)-3-cyanophenyl)-N-(4-fluoro benzyl)propanesulfonamide, N-(4-((1-formylpiperidin-4-yl)thio)-3-cyanophenyl)-N-(4-fluoro benzyl)propanesulfonamide, N-(4-((1-formylpiperidin-4-yl)amino)-3-cyanophenyl)-N-(4-fluoro benzyl)propanesulfonamide, ethyl 4-(4-(N-benzylpropanesulfonamido)2-cyanophenyl)-3-oxopiperazin-1-formate, and pharmaceutically acceptable salts or solvates thereof.

5. A method for preparing the N-benzyl-N-arylsulfonamide compound represented by formula (I) among the N-benzyl-N-arylsulfonamide derivative according to claim 1, the ring A being a substituted benzene ring and Z is absent in formula (I), the preparation method is characterized in comprising the steps of: condensing 1-halo-2-R.sub.3-4-nitrobenzene with 5- or 6-membered nitrogen-containing aliphatic heterocycle (the ring B) under a basic condition, reducing the nitro group to an amino group, subjecting the amino group to reductive amination, sulfonamidation, and deprotection if necessary, to give the target compound; alternatively, subjecting 1-halo-2-R.sub.3-4-nitrobenzene to nitro reduction, reductive amination and sulfonamidation, condensing the resultant intermediate with 5- or 6-membered nitrogen-containing aliphatic heterocycle (the ring B) under a basic condition, and deprotecting the resultant condensate if necessary, to give the target compound, the preparation method is performed according to the following synthesis scheme: ##STR00080##

6. A method for preparing the N-benzyl-N-arylsulfonamide compound represented by formula (I) among the N-benzyl-N-arylsulfonamide derivative according to claim 1, the ring A being a benzene ring, the ring B is piperidine, and Z is selected from the group consisting of O, S and NH in formula (I), the preparation method is characterized in comprising the steps of: reacting 1-halo-2-R.sub.3-4-nitrobenzene with 4-hydroxy-, 4-mercapto- or 4-amino-piperidine under a basic condition, reducing the nitro group to an amino group, and subjecting the amino group to reductive amination and sulfonamidation, to give the target compound; alternatively, reacting N-benzyl/alkyl-N-(3-R.sub.3-4-fluorphenyl)sulfonamide with N-Boc protected 4-hydroxy-, 4-mercapto- or 4-amino-piperidine, removing the Boc protecting group, and substituting at N-position of piperidine, to give the target compound, the preparation method is performed according to the following synthesis scheme: ##STR00081##

7. A method of treating autoimmune disease in a subject comprising administering to the subject the N-benzyl-N-arylsulfonamide derivative or a pharmaceutically acceptable salt or solvate thereof according to claim 1 as a selective inhibitor of Kv1.3 potassium channel.

8. The method according to claim 7, characterized in that the N-benzyl-N-arylsulfonamide derivative or the pharmaceutically acceptable salt or solvate thereof is provided in a medicament comprising with at least one pharmaceutically acceptable carrier or excipient.

9. The method according to claim 8, characterized in that the carrier or excipient is any one or more selected from the group consisting of a diluent, filler, adhesive, wetting agent, disintegrating agent, absorption accelerator, surfactant, adsorption carrier, lubricant, flavor agent and sweetener conventionally used in the field of pharmacy.

10. The method according to claim 8, characterized in that the medicament for treating autoimmune diseases is in a dosage form of tablet, capsule, patch, emulsion, suspension, gel, powder, granule, oral liquid or injection.

11. The method according to claim 8, characterized in that the salt of N-benzyl-N-arylsulfonamide compound is an inorganic acid salt or an organic acid salt, the inorganic acid salt is any one or more salts selected from the salts formed with hydrohalogen acid, nitric acid, carbonic acid, sulfuric acid and phosphoric acid, and the organic acid salt is any one or more salts selected from the salts formed with malic acid, L-malic acid, D-malic acid, citric acid, fumaric acid, oxalic acid, lactic acid, camphor sulfonic acid, L-camphor sulfonic acid, D-camphor sulfonic acid, p-toluene sulfonic acid, methane sulfonic acid and benzoic acid.

12. The method according to claim 7, characterized in that the autoimmune disease is psoriasis, psoriatic arthritis, allergic and irritant contact dermatitis, atopic dermatitis, vitiligo, rheumatoid arthritis, type I diabetes, multiple sclerosis, asthma, glomerulonephritis, periodontal disease, pars planitis, graft rejection, neurodegenerative disease, obesity, or hypertension.

13. A medicament for treating autoimmune diseases, characterized in that the medicament comprises the N-benzyl-N-arylsulfonamide derivative according to claim 1 as an active ingredient.

14. The medicament according to claim 13, characterized in that the medicament comprises at least one active component and one or more pharmaceutically acceptable carriers or excipients, and the active component is any one or more of N-benzyl-N-arylsulfonamide compounds represented by formula (I), pharmaceutically acceptable salts or solvate thereof.

15. The medicament according to claim 14, characterized in that the carrier or excipient is any one or more selected from the group consisting of a diluent, filler, adhesive, wetting agent, disintegrating agent, absorption accelerator, surfactant, adsorption carrier, lubricant, flavor agent and sweetener conventionally used in the field of pharmacy.

16. The medicament according to claim 14, characterized in that the medicament is in a dosage form of tablet, capsule, patch, emulsion, suspension, gel, powder, granule, oral liquid or injection.

17. The medicament according to claim 14, characterized in that the salt of N-benzyl-N-arylsulfonamide compound is an inorganic acid salt or an organic acid salt, the inorganic acid salt is any one or more salts selected from the group consisting of the salts formed with hydrohalogen acid, nitric acid, carbonic acid, sulfuric acid and phosphoric acid, and the organic acid salt is any one or more salts selected from the group consisting of the salts formed with malic acid, citric acid, fumaric acid, oxalic acid, lactic acid, camphor sulfonic acid, p-toluene sulfonic acid, methane sulfonic acid and benzoic acid.

18. The medicament according to claim 13, characterized in that the autoimmune disease is psoriasis, psoriatic arthritis, allergic and irritant contact dermatitis, atopic dermatitis, vitiligo, rheumatoid arthritis, type I diabetes, multiple sclerosis, asthma, glomerulonephritis, periodontal disease, pars planitis, graft rejection, neurodegenerative disease, obesity, or hypertension.

19. (canceled)

20. (canceled)

Description

DESCRIPTION OF THE FIGURES

[0103] FIG. 1 is a graph showing the effect of compound 37 on a rat model of atopic dermatitis (ACD). Compound 37 significantly reduced ear inflammation in rats with atopic dermatitis (ACD) in a manifest dose-dependent manner.

[0104] FIG. 2 is a graph showing the effect of compound 24 on a rat model of imiquimod induced psoriasis. Figure A shows the result of PASI score and Figure B shows the result of Baker score. Compound 24 exhibited a significant efficacy on psoriasis in a dose-dependent manner.

[0105] FIG. 3 is a graph showing the pathological analysis of the effect of compound 24 on a rat model of psoriasis (HE×100), in which 1) in the normal control group, the skin structure was intact and clear, without hyperkeratosis, acanthosis, capillarectasis, and with a small amount of infiltration of inflammatory cells in the dermis; 2) in the model control group, it was observed that the granular layer thinned or even disappeared, with acanthosis, thinning above papillae, and a large amount of infiltration of inflammatory cells in the dermis; 3) in the groups of compound 24, the skin structure was intact, the granular layer was visible, the skin was partly lengthened and clubbed, no acanthosis was observed, and a small amount infiltration of inflammatory cells was observed in the dermis.

DETAILED DESCRIPTION

[0106] The invention is further described with reference to examples. The following examples are merely illustrative of the invention, and not by way of limitation.

Preparation Example 1: Synthesis of ethyl 4-(4-(N-benzylphenyl sulfonamido)-2-cyanophenyl)piperazin-1-formate (compound 1)

[0107] ##STR00008## ##STR00009##

Step 1. Synthesis of ethyl 4-(2-cyano-4-nitrophenyl)piperazin-1-formate (intermediate 1a)

[0108] To a reaction flask, 2-chloro-5-nitrobenzonitrile (0.91 g, 5.0 mmol), ethyl piperzin-1-formate (0.84 g, 5.3 mmol), triethylamine (1.1 g, 10.9 mmol) and 20 mL of acetonitrile were added, and allowed for reaction under reflux for 4.5 h. After completion of the reaction, the reaction mixture was cooled to room temperature, diluted with water, and extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was recrystallized with ethyl acetate to give 1a, with a yield of 71.7%; ESI-MS: m/z=305[M+1].sup.+.

Step 2. Synthesis of ethyl 4-(4-amino-2-cyanophenyl)piperazin-1-formate (intermediate 1b)

[0109] To a reaction flask, 1a (2.76 g, 9.0 mmol), stannous chloride dihydrate (11.12 g, 49.3 mmol) and 130 mL of anhydrous ethanol were added, and allowed for reaction under reflux for 2 h. After completion of the reaction, the reaction mixture was cooled to room temperature, alkalized with a sodium carbonate solution, and suction-filtered. The filtrate was extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was recrystallized with ethyl acetate to give 1b, with a yield of 71.3%; ESI-MS: m/z=275 [M+1].sup.+.

Step 3. Synthesis of ethyl 4-(4-benzylamino-2-cyanophenyl)piperazin-1-formate (1c)

[0110] 1b (0.27 g, 1.0 mmol) was dissolved in 1 mL of acetic acid, to which benzaldehyde (0.14 g, 1.3 mmol) was added, followed by stirring at room temperature for 1 h. The reaction mixture was cooled to 10-15° C., to which sodium borohydride (0.04 g, 1.1 mmol) was added, and allowed for reaction for 10 min with the temperature maintained. Thereafter, the reaction mixture was alkalized with a sodium carbonate solution, and extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was recrystallized with ethyl acetate to give 1e, with a yield of 86.0%; ESI-MS: m/z=365[M+1].sup.+.

Step 4. Synthesis of ethyl 4-(4-(N-benzylbenzenesulfonamido)-2-cyanophenyl)piperazin-1-formate (compound 1)

[0111] 1e (0.31 g, 0.86 mmol) was dissolved in 3.0 mL of dichloromethane, to which pyridine (0.37 g, 4.7 mmol) and benzene sulfonyl chloride (0.18 g, 1.0 mmol) were added, followed by stirring at room temperature for 5 h. The reaction mixture was acidified with diluted HCl, and extracted with dichloromethane. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was recrystallized with ethyl acetate to give 1, with a yield of 77.4%; .sup.1H NMR (500 MHz, Chloroform-d): δ 7.71-7.63 (m, 3H), 7.56 (t, J=7.8 Hz, 2H), 7.27-7.23 (m, 3H), 7.22-7.19 (m, 2H), 7.16 (dd, J=7.9, 2.6 Hz, 1H), 7.09 (d, J=2.6 Hz, 1H), 6.81 (d, J=7.9 Hz, 1H), 4.68 (s, 2H), 4.18 (q, J=7.1 Hz, 2H), 3.70-3.62 (m, 4H), 3.18-3.10 (m, 4H), 1.29 (t, J=7.0 Hz, 3H); ESI-MS: m/z=505 [M+1].sup.+.

Preparation Example 2. Synthesis of ethyl 4-(2-cyano-4-((4-fluoro-N-(4-fluorobenzyl)phenyl)sulfonamido)phenyl)piperazin-1-formate (compound 2)

[0112] ##STR00010##

Step 1. Synthesis of ethyl 4-(2-cyano-4-((4-fluorobenzyl)amino) phenyl)piperazin-1-formate (2a)

[0113] The synthesis was accomplished with reference to Step 3 of Example 1, except that an intermediate 1b and p-fluorobenzaldehyde were used as raw materials to prepare compound 2a; ESI-MS: m/z=384[M+1].sup.+.

Step 2. Synthesis of ethyl 4-(2-cyano-4-((4-fluoro-N-(4-fluorobenzyl) phenyl)sulfonamido)phenyl)piperazin-1-formate (compound 2)

[0114] The synthesis was accomplished with reference to Step 4 of Example 1, except that 2a and p-fluorobenzene sulfonyl chloride were used as raw materials to prepare compound 2, with a yield of 50.2%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.72-7.65 (m, 2H), 7.22 (t, J=8.5 Hz, 2H), 7.19-7.16 (m, 2H), 7.12-7.10 (m, 2H), 6.95 (t, J=8.6 Hz, 2H), 6.83 (d, J=9.6 Hz, 1H), 4.63 (s, 2H), 4.17 (q, J=7.1 Hz, 2H), 3.70-3.60 (m, 4H), 3.20-3.11 (m, 4H), 1.28 (t, J=7.1 Hz, 3H); ESI-MS: m/z=541[M+1].sup.+.

Preparation Example 3. Synthesis of ethyl 4-(2-cyano-4-((4-fluoro-N-(4-fluoro-3-methoxybenzyl)phenyl)sulfonamido)phenyl)piperazin-1-formate (compound 3)

[0115] ##STR00011##

Step 1. Synthesis of ethyl 4-(2-cyano-4-((4-fluoro-3-methoxybenzyl) amino)phenyl)piperazin-1-formate (3a)

[0116] The synthesis was accomplished with reference to Step 3 of Example 1, except that an intermediate 1b and 4-fluoro-3-methoxy benzaldehyde were used as raw materials to prepare compound 3a, with a yield of 73.0%; ESI-MS: m/z=413[M+1].sup.+.

Step 2. Synthesis of ethyl 4-(2-cyano-4-((4-fluoro-N-(4-fluoro-3-methoxybenzyl)phenyl)sulfonamino)phenyl)piperazin-1-formate (compound 3)

[0117] The synthesis was accomplished with reference to Step 4 of Example 1, except that 3a and p-fluorobenzene sulfonyl chloride were used as raw materials to prepare compound 3, with a yield of 53.2%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.98 (m, 2H), 7.40 (dd, J=8.0, 7.5 Hz, 2H), 7.23 (dd, J=8.0, 7.5 Hz, 1H), 7.06 (d, J=7.5 Hz, 1H), 6.95 (dd, J=5.0, 1.5 Hz, 1H), 6.81 (d, J=7.5H, 1H), 6.77 (dd, J=7.5, 1.5 Hz, 1H), 6.74 (s, 1H), 4.53 (s, 2H), 4.14 (q, J=7.2 Hz, 2H), 3.83 (s, 3H), 3.32-3.29 (m, 8H), 1.22 (t, J=7.2 Hz, 3H); ESI-MS: m/z=571[M+1].sup.+.

Preparation Example 4. Synthesis of ethyl 4-(2-cyano-4-(N-(benzene sulfonamido)benzamido)phenyl)piperazin-1-formate (compound 4)

[0118] ##STR00012##

Step 1. Synthesis of ethyl 4-(4-benzamido-2-cyanophenyl)piperazin-1-formate (4a)

[0119] 1b (0.5 g, 1.82 mmol) and 4-DMAP (0.025 g, 0.2 mmol) were dissolved in 3.0 mL of pyridine, to which benzoyl chloride (0.31 g, 2.19 mmol) was added, followed by stirring at room temperature for 2 h. The reaction mixture was acidified with diluted HCl, extracted with ethyl acetate, washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (PE:EA=2:1, v/v), to give compound 4a, with a yield of 88.0%; ESI-MS: m/z=379[M+1].sup.+.

Step 2. Synthesis of ethyl 4-(2-cyano-4-(N-(benzenesulfonamido) benzamido)phenyl)piperazin-1-formate (compound 4)

[0120] 4a (0.3 g, 0.79 mmol) was dissolved in 15 mL of anhydrous THF. The resultant mixture was cooled to 0° C., to which 60% sodium hydride (0.034 g, 0.85 mmol) was added and allowed for reaction for 0.5 h with the temperature maintained, and then benzene sulfonyl chloride (0.14 g, 0.79 mmol) was added thereto. Then the resultant mixture was warmed to room temperature and allowed for reaction for 2 h. The resultant reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. The residue was purified with silica gel column chromatography (PE:EA=1:2, v/v), to give 4, with a yield of 61.0%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.97-7.94 (m, 2H), 7.70 (t, J=7.5 Hz, 1H), 7.61-7.55 (m, 2H), 7.47-7.42 (m, 2H), 7.37 (t, J=7.5 Hz, 1H), 7.34 (dd, J=7.8, 2.6 Hz, 1H), 7.29-7.27 (m, 1H), 7.25-7.22 (m, 2H), 6.89 (d, J=7.8 Hz, 1H), 4.18 (q, J=7.1 Hz, 2H), 3.70-3.65 (m, 4H), 3.25-3.17 (m, 4H), 1.29 (t, J=7.1 Hz, 3H); ESI-MS: m/z=519[M+1].sup.+.

Preparation Example 5. Synthesis of N-benzyl-N-(3-cyano-4-(4-oxopiperazin-1-yl)phenyl)benzenesulfonamide (compound 5)

[0121] ##STR00013## ##STR00014##

Step 1. Synthesis of 5-(benzylamino-2-fuorobenzonitrile (5a)

[0122] 5-Amino-2-flurobenzonitrile (1.50 g, 11.0 mmol) was dissolved in 10 mL of acetic acid, to which benzaldehyde (1.50 g, 14.1 mmol) was added, followed by stirring at room temperature for 1 h. Thereafter, the reaction mixture was cooled to 10-15° C., to which sodium borohydride (0.44 g, 11.6 mmol) was added, followed by stirring for another 10 min with the temperature maintained. Then, the reaction mixture was alkalized with a sodium carbonate solution, and extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. The residue was recrystallized with ethyl acetate to give 5a, with a yield of 80.2%; ESI-MS: m/z=227[M+1].sup.+.

Step 2. Synthesis of N-benzyl-N-(3-cyano-4-fluorophenyl) benzenesulfonamide (5b)

[0123] 5a (1.54 g, 6.8 mmol) was dissolved in 20 mL of dichloromethane, to which pyridine (2.96 g, 37.4 mmol) and benzene sulfonyl chloride (1.44 g, 8.2 mmol) were added, followed by stirring at room temperature for 5 h. The reaction mixture was acidified with diluted HCl, extracted with dichloromethane, washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was recrystallized with ethyl acetate to give 5b, with a yield of 87.8%; ESI-MS: m/z=337[M+1].sup.+.

Step 3. Synthesis of N-benzyl-N-(3-cyano-4-(1,4-dioxa-8-azaspiro [4.5]dec-8-yl)phenyl)benzenesulfonamide (5c)

[0124] To a reaction flask, 5b (0.20 g, 0.55 mmol), 4-piperidone ethylene glycol ketal hydrochloride (0.11 g, 0.62 mmol), anhydrous potassium carbonate (0.22 g, 1.61 mmol) and 2 mL of DMSO were added, and allowed for reaction at 120° C. for 6 h. After completion of the reaction, the resultant mixture was cooled to room temperature, diluted with water, extracted with ethyl acetate, and washed with saturated saline. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (PE:EA=2:1, v/v) to give 5c, with a yield of 86.7%; ESI-MS: m/z=490[M+1].sup.+.

Step 4. Synthesis of N-benzyl-N-(3-cyano-4-(4-oxopiperidin-1-yl)phenyl) benzenesulfonamide (compound 5)

[0125] 5c (0.20 g, 0.41 mmol) was dissolved in 3 mL of THF, to which 3 mL of 10% diluted sulfuric acid solution was added, followed by stirring at room temperature for 5 h. The reaction mixture was alkalinized with a sodium carbonate solution, extracted with ethyl acetate, washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (PE:EA=1:1, v/v) to give 50, with a yield of 87.9%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.71-7.65 (m, 3H), 7.57 (t, J=7.5 Hz, 2H), 7.25-7.23 (m, 3H), 7.22-7.20 (m, 2H), 7.18 (dd, J=7.8, 2.6 Hz, 1H), 7.12 (d, J=2.6 Hz, 1H), 6.87 (d, J=7.8 Hz, 1H), 4.69 (s, 2H), 3.50 (t, J=6.0 Hz, 4H), 2.66 (t, J=6.0 Hz, 4H); ESI-MS: m/z=446[M+1].sup.+.

Preparation Example 6. Synthesis of N-benzyl-N-(3-cyano-4-(4-hydroxypiperidin-1-yl)phenyl)benzenesulfonamide (compound 6)

[0126] ##STR00015##

[0127] Steps: 5b (0.50 g, 1.36 mol), 4-piperidinol (1.58 g, 1.56 mmol) and potassium carbonate (0.54 g, 3.9 mmol) were dissolved in 5 mL of DMF, warmed to 100° C., and allowed for reaction for 2 h. After completion of the reaction, the reaction mixture was cooled to room temperature, diluted with water, extracted with ethyl acetate, washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (DCM:EA=10:1, v/v) to give 6, with a yield of 70.4%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.71-7.63 (m, 3H), 7.55 (t, J=7.5 Hz, 2H), 7.25 (m, 1H), 7.24-7.21 (m, 2H), 7.20-7.18 (m, 2H), 7.12 (dd, J=7.9, 2.6 Hz, 1H), 7.04 (d, J=2.6 Hz, 1H), 6.82 (d, J=7.9 Hz, 1H), 4.67 (s, 2H), 4.03-3.84 (m, 1H), 3.52-3.40 (m, 2H), 3.09-2.93 (m, 2H), 2.15-1.97 (m, 2H), 1.84-1.67 (m, 2H); ESI-MS: m/z=448[M+1].sup.+.

Preparation Example 7. Synthesis of N-benzyl-N-(3-cyano-4-(4-acetyloxypiperidin-1-yl)phenyl)benzenesulfonamide (compound 7)

[0128] ##STR00016##

[0129] Steps: Compound 6 (0.25 g, 0.56 mmol) was dissolved in 20 mL of dichloromethane, to which triethylamine (0.084 g, 0.83 mmol) and acetyl chloride (0.052 g, 0.67 mmol) were added, followed by stirring at room temperature for 1 h. After completion of the reaction, the organic layer was washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (PE:EA=2:1, v/v) to give 7, with a yield of 51.2%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.70-7.64 (m, 3H), 7.55 (t, J=7.8 Hz, 2H), 7.25-7.21 (m, 3H), 7.20-7.17 (m, 2H), 7.13 (dd, J=7.9, 2.6 Hz, 1H), 7.05 (d, J=2.6 Hz, 1H), 6.83 (d, J=7.9 Hz, 1H), 5.00-4.91 (m, 1H), 4.67 (s, 2H), 3.44-3.30 (m, 2H), 3.12-3.04 (m, 2H), 2.08 (s, 3H), 2.07-2.02 (m, 2H), 1.92-1.81 (m, 2H); ESI-MS: m/z=490[M+1].sup.+.

Preparation Example 8. Ethyl 4-(2-cyano-4-((4-fluoro-N-propylphenyl) sulfonamido)phenyl)piperazin-1-formate (compound 8)

[0130] ##STR00017##

Step 1. Synthesis of ethyl 4-(2-cyano-4-(propylamino)phenyl)piperazin-1-formate (8a)

[0131] 1b (0.3 g, 1.1 mmol) was dissolved in 30 mL of anhydrous ethanol, to which propionaldehyde (0.095 g, 1.6 mmol) and 0.3 g of molecular sieve were added, and allowed for reaction under reflux for 4 h. Thereafter, the reaction mixture was cooled to room temperature, to which sodium borohydride (0.05 g, 1.3 mmol) was added, and allowed for reaction at room temperature for another 1 h. The resultant reaction mixture was diluted with water, extracted with ethyl acetate, washed with saturated saline, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (DCM:EA=5:1, v/v) to give 8a, with a yield of 38.1%; ESI-MS: m/z=317[M+1].sup.+.

Step 2. Synthesis of ethyl 4-(2-cyano-4-((4-fluoro-N-propylphenyl) sulfonamido)phenyl)piperazin-1-formate (compound 8)

[0132] The synthesis was accomplished with reference to Step 4 of Example 1, except that 8a and p-fluorobenzene sulfonyl chloride were used as raw materials to prepare compound 8, with a yield of 49.3%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.65-7.60 (m, 2H), 7.25 (dd, J=8.8, 2.6 Hz, 1H), 7.22-7.16 (m, 3H), 6.94 (d, J=8.8 Hz, 1H), 4.19 (q, J=7.1 Hz, 2H), 3.73-3.67 (m, 4H), 3.45 (t, J=7.1 Hz, 2H), 3.24-3.18 (m, 4H), 1.50-1.38 (m, 2H), 1.30 (t, J=7.1 Hz, 3H), 0.91 (t, J=7.4 Hz, 3H); ESI-MS: m/z=475[M+1].sup.+.

Preparation Example 9. Synthesis of ethyl 4-(2-cyano-4-(N-(4-fluorobenzyl)propanesulfonamido)phenyl)piperazin-1-formate (compound 9)

[0133] ##STR00018##

[0134] The synthesis was accomplished with reference to Step 4 of Example 1, except that 2a and propane sulfonyl chloride were used as raw materials to prepare compound 9 and the crude product was purified with silica gel column chromatography (PE:EA=2:1, v/v), with a yield of 45.1%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.43 (d, J=2.6 Hz, 1H), 7.31 (dd, J=7.8, 2.6 Hz, 1H), 7.23-7.18 (m, 2H), 6.97-6.94 (m, 2H), 6.89 (d, J=8.9 Hz, 1H), 4.77 (s, 2H), 4.17 (q, J=7.1 Hz, 2H), 3.69-3.63 (m, 4H), 3.19-3.14 (m, 4H), 3.05-2.99 (m, 2H), 1.98-1.85 (m, 2H), 1.28 (t, J=7.1 Hz, 3H), 1.08 (t, J=7.4 Hz, 3H); ESI-MS: m/z=489[M+1].sup.+.

Preparation Example 10. Synthesis of N-(3-cyano-4-morpholinyl phenyl)-N-(4-fluorobenzyl)benzenesulfonamide (compound 10)

[0135] ##STR00019##

Step 1. Synthesis of 2-morpholinyl-5-nitrobenzonitrile (10a)

[0136] 2-Bromo-5-nitrobenzonitrile (1.0 g, 4.4 mmol) was dissolved in 20 mL of tetrahydrofuran, to which potassium carbonate (1.22 g, 8.8 mmol) and morpholine (0.58 g, 6.6 mmol) were added. The resultant mixture was heated to reflux for 4 h. After completion of the reaction, the mixture was cooled to room temperature, and filtered. The filtrate was concentrated, and then purified with silica gel column chromatography (PE:EA=4:1, v/v) to give 10a, with a yield of 96.3%; ESI-MS: m/z=234[M+1].sup.+.

Step 2. Synthesis of 2-morpholinyl-5-aminobenzonitrile (10b)

[0137] 10a (1.0 g, 4.29 mmol) was dissolved in 10 mL of anhydrous ethanol, to which stannous chloride dihydrate (4.84 g, 21.4 mmol) was added. The resultant mixture was heated to reflux for 6 h. After completion of the reaction, the reaction mixture was cooled to room temperature, alkalized with a sodium bicarbonate solution, and filtered. The filtrate was extracted with ethyl acetate. The organic layers were combined, washed with saturated saline, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was recrystallized with ethyl acetate, to give 10b, with a yield of 51.6%; ESI-MS: m/z=204[M+1].sup.+.

Step 3. Synthesis of 5-((4-fluorobenzyl)amino)-2-morpholinylbenzonitrile (10c)

[0138] 10b (0.48 g, 2.36 mol) was dissolved in 10 mL of acetic acid, to which p-fluorobenzaldehyde (0.35 g, 2.83 mmol) was added, followed by stirring at room temperature for 1 h, and to which sodium borohydride (0.134 g, 3.54 mmol) was added at 10-15° C., and allowed for reaction for another 0.5 h with the temperature maintained. After completion of the reaction, the reaction mixture was poured into water, alkalized with a sodium bicarbonate solution, and extracted with ethyl acetate. The organic layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was recrystallized with ethyl acetate to give 10c, with a yield of 27.2%; ESI-MS: m/z=312[M+1].sup.+.

Step 4. Synthesis of N-(3-cyano-4-morpholinylphenyl)-N-(4-fluorobenzyl) benzenesulfonamide (compound 10)

[0139] 10c (0.2 g, 0.64 mmol) was dissolved in 10 mL of dichloromethane, to which pyridine (0.25 g, 3.2 mmol) and propane sulfonyl chloride (0.15 g, 0.82 mmol) were added, and allowed for reaction at room temperature for 6 h. After completion of the reaction, the resultant mixture was poured into water. The organic phase was washed with diluted HCl solution, and extracted with dichloromethane. The organic phases were combined, washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified with silica gel column chromatography (PE:EA=4:1, v/v), to give 10, with a yield of 74.7%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.70-7.64 (m, 3H), 7.56 (t, J=7.8 Hz, 2H), 7.21-7.16 (m, 2H), 7.14 (dd, J=7.9, 2.6 Hz, 1H), 7.05 (d, J=2.6 Hz, 1H), 6.99-6.92 (m, 2H), 6.83 (d, J=8.9 Hz, 1H), 4.64 (s, 2H), 3.94-3.79 (m, 4H), 3.33-3.12 (m, 4H); ESI-MS: m/z=452[M+1].sup.+.

Preparation Example 11. Synthesis of 4-(4-(N-benzylbenzenesulfonamido)-2-cyanophenyl)-N,N-dimethylpiperazin-1-formamide (compound 11)

[0140] ##STR00020##

[0141] Steps: To a reaction flask, 5b (0.106 g, 0.29 mol), N,N-dimethylpiperazin-1-formamide (0.05 mg, 0.32 mmol), potassium carbonate (0.12 g, 0.87 mmol) and 2 mL of DMF were added, warmed to 100° C., and allowed for reaction for 2 h. After completion of the reaction, the reaction mixture was cooled to room temperature, diluted with water, extracted with ethyl acetate, washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified with silica gel column chromatography (DCM:MeOH=20:1), to give 11, with a yield of 65.2%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.71-7.64 (m, 3H), 7.55 (t, J=7.8 Hz, 2H), 7.27-7.22 (m, 3H), 7.23-7.18 (m, 2H), 7.13 (dd, J=7.9, 2.6 Hz, 1H), 7.09 (d, J=2.6 Hz, 1H), 6.81 (d, J=7.9 Hz, 1H), 4.67 (s, 2H), 3.48-3.40 (m, 4H), 3.23-3.13 (m, 4H), 2.86 (s, 6H); ESI-MS: m/z=504[M+1].sup.+.

Preparation Example 12. Synthesis of 1-(4-(N-benzylbenzene sulfonamido)-2-cyanophenyl)-N,N-dimethylpiperidin-4-sulfonamide (compound 12)

[0142] ##STR00021##

[0143] The synthesis was accomplished with reference to Example 11, except that 5b and N,N-dimethylpiperidin-4-sulfonamide were used as raw materials to prepare compound 12, with a yield of 78.0%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.71-7.64 (m, 3H), 7.56 (t, J=7.8 Hz, 2H), 7.27-7.22 (m, 3H), 7.22-7.19 (m, 2H), 7.15 (dd, J=7.8, 2.6 Hz, 1H), 7.07 (d, J=2.6 Hz, 1H), 6.81 (d, J=7.8 Hz, 1H), 4.67 (s, 2H), 3.71-3.66 (m, 2H), 3.16-3.05 (m, 1H), 2.97 (s, 6H), 2.87-2.76 (m, 2H), 2.20-2.15 (m, 2H), 2.13-2.03 (m, 2H); ESI-MS: m/z=539[M+1].sup.+.

Preparation Example 13. Synthesis of ethyl 4-(2-cyano-4-(N-isobutylphenylsulfonamido)phenyl)piperazin-1-formate (compound 13)

[0144] ##STR00022##

Step 1. Synthesis of ethyl 4-(2-cyano-4-(isobutylamino)phenyl) piperazin-1-formate (13a)

[0145] The synthesis was accomplished with reference to Step 1 of Example 8, except that 1b and isobutyraldehyde were used as raw materials to prepare compound 13a, and the residue was purified with silica gel column chromatography (DCM:EA=5:1, v/v), with a yield of 40.1%; ESI-MS: m/z=−331[M+1].sup.+.

Step 2. Synthesis of ethyl 4-(2-cyano-4-(N-isobutylphenylsulfonamido) phenyl)piperazin-1-formate (compound 13)

[0146] The synthesis was accomplished with reference to Step 4 of Example 1, except that 13a and benzene sulfonyl chloride were used as raw materials to prepare compound 13, with a yield of 63.4%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.65-7.60 (m, 1H), 7.65-7.62 (m, 1H), 7.60-7.56 (m, 2H), 7.52-7.49 (m, 2H), 7.30-7.28 (m, 1H), 7.13 (d, J=2.6 Hz, 1H), 6.94 (d, J=7.8 Hz, 1H), 4.19 (q, J=7.1 Hz, 2H), 3.73-3.67 (m, 4H), 3.26 (d, J=7.4 Hz, 2H), 3.23-3.17 (m, 4H), 1.59-1.50 (m, 1H), 1.30 (t, J=7.1 Hz, 3H), 0.92 (d, J=6.6 Hz, 6H); ESI-MS: m/z=471[M+1].sup.+.

Preparation Example 14. Synthesis of ethyl 1-(4-(N-benzylbenzene sulfonamido)-2-cyanophenyl)piperidin-4-yl carbamate (compound 14)

[0147] ##STR00023##

[0148] The synthesis was accomplished with reference to Step 3 of Example 5, except that 5b and ethyl piperidin-4-carbamate were used as raw materials to prepare compound 14, with a yield of 65%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.70-7.63 (m, 3H), 7.55 (t, J=7.8 Hz, 2H), 7.27-7.23 (m, 3H), 7.22-7.18 (m, 2H), 7.12 (dd, J=7.9, 2.5 Hz, 1H), 7.05 (d, J=2.5 Hz, 1H), 6.81 (d, J=7.9 Hz, 1H), 4.67 (s, 2H), 4.60 (brs, 1H), 4.13 (q, J=7.0 Hz, 2H), 3.72-3.62 (m, 1H), 3.53-3.47 (m, 2H), 2.94-2.84 (m, 2H), 2.11-2.05 (m, 2H), 1.65-1.60 (m, 2H), 1.26 (t, J=7.0 Hz, 3H); ESI-MS: m/z=519[M+1].sup.+.

Preparation Example 15. Synthesis of N-(1-(4-(N-benzylbenzene sulfonamido)-2-cyanophenyl)pyrrolidin-3-yl)acetamide (compound 15)

[0149] ##STR00024##

[0150] The synthesis was accomplished with reference to Step 3 of Example 5, except that 5b and N-(pyrrolidin-3-yl)acetamide were used as raw materials to prepare compound 15, with a yield of 97.0%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.69 (d, J=8.0 Hz, 2H), 7.65 (t, J=6.9 Hz, 1H), 7.54 (t, J=7.7 Hz, 2H), 7.25-7.20 (m, 5H), 7.01-6.97 (m, 1H), 6.93 (d, J=2.5 Hz, 1H), 6.44 (dd, J=8.0, 2.5 Hz, 1H), 5.82 (d, J=7.0 Hz, 1H), 4.64 (s, 2H), 4.62-4.51 (m, 1H), 3.86-3.66 (m, 2H), 3.67-3.52 (m, 1H), 3.42-3.39 (m, 1H), 2.38-2.16 (m, 1H), 2.01-1.92 (m, 4H); ESI-MS: m/z=475[M+1].sup.+.

Preparation Example 16. Synthesis of N-benzyl-N-(3-cyano-4-(2,6-dioxopiperidin-1-yl)phenyl)benzenesulfonamide (compound 16)

[0151] ##STR00025##

Step 1. Synthesis of 2-(2,6-dioxopiperidin-1-yl)-5-nitrobenzonitrile (16a)

[0152] Glutarimide (0.94 g, 8.31 mmol) was dissolved in 5 mL of anhydrous DMF and cooled to 0-5° C., to which 60% sodium hydride (0.33 g, 8.31 mmol) was added in batches, followed by stirring for 0.5 h with the temperature maintained, and to which 2-fluoro-5-nitrobenzonitrile (1.25 g, 7.55 mmol) was further added, warmed to 50° C., and allowed for reaction overnight. After completion of the reaction, the reaction mixture was cooled to room temperature, diluted with water, extracted with ethyl acetate, washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (PE:EA=1:1, v/v), to give 16a, with a yield of 25.1%; ESI-MS: m/z=260[M+1].sup.+.

Step 2. Synthesis of 5-(benzylamino)-2-(2,6-dioxopiperidin-1-yl)benzonitrile (16c)

[0153] 16a (0.25 g, 0.97 mmol) was dissolved in 6 mL of acetic acid, to which 0.05 g of 10% palladium on carbon was added, and allowed for reduction for 1 h at 30° C. with the addition of hydrogen. After completion of the reaction, the reaction mixture was filtered under reduced pressure. To the filtrate, benzaldehyde (0.13 g, 1.24 mmol) was added, and allowed for reaction at room temperature for 1 h, and then cooled to 10-15° C., to which sodium borohydride (0.055 g, 1.45 mmol) was added, and allowed for reaction for 0.5 h with the temperature maintained. After completion of the reaction, the reaction mixture was poured into water, alkalized with sodium bicarbonate, and extracted with ethyl acetate. The organic phases were combined, washed with saturate saline, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (DCM:EA=15:1, v/v), to give 16c. The yield for the two steps was 47.5%; ESI-MS: m/z=320[M+1].sup.+.

Step 3. Synthesis of N-benzyl-N-(3-cyano-4-(2,6-dioxopiperidin-1-yl) phenyl)benzenesulfonamide (compound 16)

[0154] 23c (0.14 g, 0.46 mmol) was dissolved in 10 mL of dichloromethane, to which pyridine (0.2 g, 2.53 mmol) and benzene sulfonyl chloride (0.1 g, 0.59 mmol) were further added, and allowed for reaction at room temperature for 1 h. After completion of the reaction, the organic phase was washed with diluted HCl solution, and extracted with dichloromethane. The organic phases were combined, washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (PE:EA=1:1, v/v), to give 16, with a yield of 30.6%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.71-7.63 (m, 3H), 7.55 (t, J=7.5, 2H), 7.37 (dd, J=8.0, 2.5 Hz, 1H), 7.34 (d, J=2.5 Hz, 1H), 7.32-7.28 (m, 1H), 7.25-7.21 (m, 4H), 7.13 (d, J=8.0 Hz, 1H), 4.76 (s, 2H), 2.95-2.84 (m, 2H), 2.84-2.71 (m, 2H), 2.29-2.07 (m, 2H); ESI-MS: m/z=460[M+1].sup.+.

Preparation Example 17. Synthesis of N-benzyl-N-(3-cyano-4-(3-methyl-2-oxotetrahydropyrimidin-1(2H)-yl)phenyl)benzenesulfonamide (compound 17)

[0155] ##STR00026##

Step 1. Synthesis of 5-(benzylamino)-2-bromobenzonitrile (17a)

[0156] The synthesis was accomplished with reference to Step 3 of Example 5, except that 5-amino-2-bromobenzonitrile and benzaldehyde were used as raw materials to prepare compound 17a, with a yield of 85.8%. ESI-MS: m/z=288[M+1].sup.+.

Step 2. Synthesis of N-benzyl-N-(4-bromo-3-cyanophenyl)benzenesulfonamide (17b)

[0157] The synthesis was accomplished with reference to Step 4 of Example 5, except that 17a and benzene sulfonyl chloride were used as raw materials to prepare compound 17b, with a yield of 85.0%; ESI-MS: m/z=288[M+1].sup.+.

Step 3. Synthesis of N-benzyl-N-(3-cyano-4-(3-methyl-2-oxotetrahydropyrimidin-1(2H)-yl)phenyl)benzensulfonamide (compound 17)

[0158] To a reaction flask, 17b (0.427 g, 1.0 mmol), 0.137 g (1.2 mmol) of 1-methyltetrahydro-2(1H)-pyrimidinone (0.137 g, 1.2 mmol), potassium carbonate (0.276 g, 2.0 mmol), copper iodide (0.019 g, 0.1 mmol) and trans-N,N′-dimethyl-1,2-cyclohexanediamine (0.015 g, 0.1 mmol) and 1 mL of toluene were added, and allowed for reaction at 110° C. under the protection of nitrogen gas for 24 h. After completion of the reaction, the reaction mixture was cooled to room temperature, and filtered. The filtrate was concentrated under reduced pressure. The residue was purified with silica gel column chromatography (DCM:MeOH=20:1), to give 17, with a yield of 11.1%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.70-7.68 (s, 2H), 7.66 (t, J=7.5 Hz, 1H), 7.55 (t, J=7.7 Hz, 2H), 7.26-7.21 (m, 7H), 7.19 (d, J=2.4 Hz, 1H), 4.71 (s, 2H), 3.67 (t, J=6.4 Hz, 2H), 3.42 (t, J=6.1 Hz, 2H), 3.01 (s, 3H), 2.27-2.10 (m, 2H); ESI-MS: m/z=460[M+1].sup.+.

Preparation Example 18. Synthesis of N-benzyl-N-(3-cyano-4-(4-(2-(dimethylamino)ethoxy)piperidin-1-yl)phenyl)benzenesulfonamide (compound 18)

[0159] ##STR00027##

[0160] The synthesis was accomplished with reference to Step 3 of Example 5, except that 5b and N,N-dimethyl-2-(4-piperidyloxy)ethylamine were used as raw materials to prepare compound 18, and the residue was purified with silica gel column chromatography (DCM:MeOH:TEA=40:1:1), to give 18, with a yield of 68%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.70-7.63 (m, 3H), 7.55 (t, J=7.8 Hz, 2H), 7.26-7.22 (m, 3H), 7.22-7.19 (m, 2H), 7.10 (dd, J=8.9, 2.6 Hz, 1H), 7.02 (d, J=2.6 Hz, 1H), 6.80 (d, J=8.9 Hz, 1H), 4.66 (s, 2H), 3.60 (t, J=5.9 Hz, 2H), 3.54-3.48 (m, 1H), 3.45-3.37 (m, 2H), 3.06-2.95 (m, 2H), 2.55 (t, J=5.9 Hz, 2H), 2.31 (s, 6H), 2.04-1.94 (m, 2H), 1.87-1.75 (m, 2H); ESI-MS: m/z=519[M+1].sup.+.

Preparation Example 19. Synthesis of N-(3-cyano-4-(4-hydroxypiperidin-1-yl)phenyl)-N-(4-fluorobenzyl)propane-1-sulfonamide (compound 19)

[0161] ##STR00028## ##STR00029##

Step 1. Synthesis of 2-(4-hydroxypiperidin-1-yl)-5-nitrobenzonitrile (19a)

[0162] 2-Chloro-5-nitrobenzonitrile (1.5 g, 8.21 mmol), 4-hydroxypiperidine (0.87 g, 8.63 mmol) and triethylamine (1.25 g, 12.3 mmol) were dissolved in 20 mL of acetonitrile, warmed to 80° C. and allowed for reaction for 2 h. The resultant mixture was concentrated under reduced pressure. The residue was purified with silica gel column chromatography (DCM:MeOH=20:1, v/v), to give 19a, with a yield of 74.4%; ESI-MS: m/z=248[M+1].sup.+.

Step 2. Synthesis of 1-(2-cyano-4-nitrophenyl)piperidin-4-ylacetate (19b)

[0163] 19a (1.2 g, 4.85 mmol) was dissolved in 20 mL of DCM, to which triethylamine (0.74 g, 7.28 mmol) was added. The resultant mixture was then cooled to 0-5° C., to which acetyl chloride (0.46 g, 5.82 mmol) was added dropwise. Thereafter, the mixture was warmed to room temperature, and allowed for reaction for 1 h. The resultant mixture was concentrated under reduced pressure. The residue was purified with silica gel column chromatography (PE:EA=1:1, v/v), to give 19b, with a yield of 54.1%; ESI-MS: m/z=290[M+1].sup.+.

Step 3. Synthesis of 1-(4-amino-2-cyanophenyl)piperidin-4-ylacetate (19c)

[0164] 19b (0.75 g, 2.59 mmol) and stannous chloride dihydrate (2.93 g, 12.96 mmol) were dissolved in 20 mL of ethanol, and allowed for reaction under reflux for 2 h. After completion of the reaction, the reaction mixture was cooled to room temperature, alkalized with a sodium carbonate solution, and filtered. The filtrate was extracted with ethyl acetate, washed with saturate saline, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (PE:EA=2:1, v/v), to give 19c, with a yield of 81.8%; ESI-MS: m/z=260[M+1].sup.+.

Step 4. Synthesis of 1-(2-cyano-4-((4-fluorobenzyl)amino)phenyl) piperidin-4-ylacetate (19d)

[0165] The synthesis was accomplished with reference to Step 3 of Example 1, except that the intermediate 19c and p-fluorobenzaldehyde were used as raw materials to prepare compound 19d, and the obtained crude product was purified with silica gel column chromatography (PE:EA=2:1, v/v), to give 19d, with a yield of 81.1%; ESI-MS: m/z=368[M+1].sup.+.

Step 5. Synthesis of 1-(2-cyano-4-(N-(4-fluorobenzyl)propylsulfonamido) phenyl)piperidin-4-ylacetate (19e)

[0166] The synthesis was accomplished with reference to Step 2 of Example 2, except that the intermediate 19d and propanesulfonyl chloride were used as raw materials to prepare compound 19e, and the obtained crude product was purified with silica gel column chromatography (PE:EA=2:1, v/v), to give 19e, with a yield of 69.8%; ESI-MS: m/z=474[M+1].sup.+.

Step 6. Synthesis of N-(3-cyano-4-(4-hydroxypiperidin-1-yl)phenyl)-N-(4-fluorobenzyl)propane-1-sulfonamide (compound 19)

[0167] 19e (0.16 g, 0.34 mmol) and lithium hydroxide (0.033 g, 1.35 mmol) were dissolved in a mixture of 4 mL of THF and 1 mL of water, followed by stirring at room temperature overnight, and then diluted with water, extracted with ethyl acetate, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified with silica gel column chromatography (DCM:EA=10:1, v/v), to give 19, with a yield of 96.0%. 1H NMR (500 MHz, Chloroform-d): δ 7.39 (d, J=2.6 Hz, 1H), 7.24 (dd, J=7.9, 2.6, 1H), 7.24-7.19 (m, 2H), 7.00-6.96 (m, 2H), 6.90 (d, J=7.9 Hz, 1H), 4.76 (s, 2H), 4.13-3.73 (m, 1H), 3.59-3.46 (m, 2H), 3.13-2.85 (m, 4H), 2.22-1.97 (m, 2H), 2.02-1.83 (m, 2H), 1.84-1.71 (m, 2H), 1.09 (t, J=7.4 Hz, 3H); ESI-MS: m/z=432[M+1].sup.+.

Preparation Example 20. Synthesis of N-(3-cyano-4-(4-hydroxypiperidin-1-yl)phenyl)-N-(4-fluorobenzyl)benzenesulfonamide (compound 20)

[0168] ##STR00030##

Step 1. Synthesis of 2-fluoro-5-((4-fluorobenzyl)amino)benzonitrile (20a)

[0169] The synthesis was accomplished with reference to Step 1 of Example 5, except that 5b and p-fluorobenzaldehyde were used as raw materials to prepare compound 20a, with a yield of 91.1%. ESI-MS: m/z=245[M+1].sup.+.

Step 2. Synthesis of N-(3-cyano-4-fluorophenyl)-N-(4-fluorobenzyl) benzenesulfonamide (20b)

[0170] The synthesis was accomplished with reference to Step 2 of Example 5, except that 5c and benzene sulfonyl chloride were used as raw materials to prepare compound 20b, with a yield of 89.0%; ESI-MS: m/z=385[M+1].sup.+.

Step 3. Synthesis of N-(3-cyano-4-(4-hydroxypiperidin-1-yl)phenyl)-N-(4-fluorobenzyl)benzenesulfonamide (compound 20)

[0171] The synthesis was accomplished with reference to Example 11, except that 20b and 4-hydroxypiperidine were used as raw materials to prepare compound 20, and the obtained crude product was purified with silica gel column chromatography (DCM:EA=10:1, v/v), with a yield of 96%. 1H NMR (500 MHz, Chloroform-d): δ 7.71-7.62 (m, 3H), 7.59-7.51 (m, 2H), 7.21-7.15 (m, 2H), 7.11-7.04 (m, 1H), 7.04-6.99 (m, 1H), 6.98-6.90 (m, 2H), 6.83 (dd, J=7.9, 2.6 Hz, 1H), 4.63 (s, 2H), 3.95-3.81 (m, 1H), 3.63-3.41 (m, 2H), 3.11-2.93 (m, 2H), 2.14-1.98 (m, 2H), 1.84-1.67 (m, 2H); ESI-MS: m/z=466[M+1].sup.+.

Preparation Example 21. Synthesis of 4-(2-cyano-4-(N-(4-fluorobenzyl) propyl)phenyl)-N,N-dimethylpiperazin-1-formamide (compound 21)

[0172] ##STR00031## ##STR00032##

Step 1. Synthesis of 5-nitro-2-(piperazin-1-yl)benzonitrile (21a)

[0173] The synthesis was accomplished with reference to Step 1 of Example 1, except that 2-fluoro-5-nitrobenzonitrile and piperazine were used as raw materials to prepare compound 21a, with a yield of 98.2%. ESI-MS: m/z=233[M+1].sup.+.

Step 2. Synthesis of 4-(2-cyano-4-nitrophenyl)-N,N-dimethylpiperazin-1-formamide (21b)

[0174] 21a (0.5 g, 2.15 mmol) was dissolved in 10 mL of DCM, to which triethylamine (0.65 g, 6.46 mmol) was added. The resultant mixture was cooled to 0-5° C., to which dimethylamino formal chloride (0.35 g, 3.23 mmol) was added dropwise. Thereafter, the resultant mixture was warmed to room temperature, and allowed for reaction for 2 h. After completion of the reaction, the reaction mixture was concentrated under reduced pressure. The residue was purified with silica gel column chromatography (DCM:MeOH=20:1, v/v), to give 21b, with a yield of 91%; ESI-MS: m/z=304[M+1].sup.+.

Step 3. Synthesis of 4-(4-amino-2-cyanophenyl)-N,N-dimethylpiperazin-1-formamide (21c)

[0175] The synthesis was accomplished with reference to Step 2 of Example 1, except that 21b was used as raw material to prepare compound 21c, and the obtained crude product was purified with silica gel column chromatography (DCM:MeOH=20:1, v/v), with a yield of 87.6%; ESI-MS: m/z=248[M+1].sup.+.

Step 4. Synthesis of 4-(2-cyano-4-((4-fluorobenzyl)amino)phenyl)-N,N-dimethylpiperazin-1-formamide (21d)

[0176] The synthesis was accomplished with reference to Step 3 of Example 1, except that 21c and p-fluorobenzaldehyde were used as raw materials to prepare compound 21d, and the obtained crude product was purified with silica gel column chromatography (DCM:MeOH=20:1, v/v), with a yield of 86.0%; ESI-MS: m/z=382[M+1].sup.+.

Step 5. Synthesis of 4-(2-cyano-4-(N-(4-fluorobenzyl)propyl)phenyl)-N,N-dimethylpiperazin-1-formamide (compound 21)

[0177] The synthesis was accomplished with reference to Step 4 of Example 1, except that 21d and propane sulfonyl chloride were used as raw materials to prepare compound 21, and the obtained crude product was purified with silica gel column chromatography (PE:EA=2:1, v/v), with a yield of 46.9%. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.42 (d, J=2.5 Hz, 1H), 7.30 (dd, J=7.9, 2.5 Hz, 1H), 7.24-7.16 (m, 2H), 6.98 (t, J=8.6 Hz, 2H), 6.89 (d, J=7.9 Hz, 1H), 4.77 (s, 2H), 3.56-3.37 (m, 4H), 3.27-3.14 (m, 4H), 3.09-2.94 (m, 2H), 2.86 (s, 6H), 1.99-1.84 (m, 2H), 1.09 (t, J=7.4 Hz, 3H); ESI-MS: m/z=488[M+1].sup.+.

Preparation Example 22. Synthesis of compounds 22-36

[0178] ##STR00033##

Step 1. Synthesis of intermediates 22a-36a

[0179] The synthesis was accomplished with reference to Step 3 of Example 1, except that 1b and corresponding aromatic aldehyde were used as raw materials to prepare compounds 22a-36a.

Step 2. Synthesis of compounds 22-36

[0180] The synthesis was accomplished with reference to Step 3 of Example 3, except that 22a-36a and propane sulfonyl chloride were used as raw materials to prepare compounds 22-36.

TABLE-US-00001 TABLE 1.1 NMR and MS data of compounds 22-36 Preparation Example Compound No. Name and structure of the compound .sup.1H NMR and MS(ESI) Compound 22 Ethyl 4-(4-(N-benzylpropanesulfonamido) 2-cyanophenyl)piperazin-1-formate [00034] .sup.1HNMR(500 MHz, Chloroform-d): δ 7.31-7.29(m, 5H), 6.81(d, J = 7.5 Hz, 1H), 6.77(d, J = 7.5 Hz, 1H), 6.74(s, 1H), 4.56(s, 2H), 4.14(q, J = 8.0 Hz, 2H), 3.32-3.29(m, 8H), 3.11(d, J = 7.1Hz, 2H), 1.69(m, 2H), 1.22(t, J = 8.0Hz, 3H), 1.01(t, J = 7.9Hz, 3H); ESI-MS: m/z = 471[M + 1].sup.+ Compound 23 Ethyl 4-(2-cyano-4-(N-(3-fluoro benzyl)propanesulfonamido)phenyl) piperazin-l-formate [00035] .sup.1H NMR (500 MHz, Chloroform-d): δ 7.36(m, 1H), 7.09(m, 1H), 6.97(d, J = 8.0 Hz, 1H), 6.81-6.74(m, 3H), 4.58(s, 1H), 4.15(q, J = 7.8 Hz, 2H) 3.33-3.30(m, 8H), 3.10(d, J = 7.1 Hz, 2H), 1.70(m, 2H), 1.24(t, J = 7.5 Hz, 3H), 0.99(t, J = 7.2 Hz, 3H); ESI-MS: m/z = 489 [M + 1].sup.+ Compound 24 Ethyl 4-(4-(4-(N-(4-chlorobenzyl) propanesulfonamido)2-cyanophenyl) piperazin-l-formate [00036] .sup.1HNMR(500 MHz, Chloroform-d): δ 7.48 (d, J = 7.5 Hz, 2H), 7.32(d, J = 7.5 Hz, 2H), 6.83(d, J = 7.5 Hz, 1H), 6.78(d, J = 7.5 Hz, 1H), 6.74(s, 1H), 4.58(s, 2H), 4.14(q, J = 7.9 Hz, 2H), 3.34-3.30(m, 8H), 3.12(d, J = 7.2 Hz, 2H), 1.70(m, 2H), 1.24(t, J = 7.2 Hz, 3H), 1.01(t, J = 7.2 Hz, 3H); ESI-MS: m/z = 506 [M + 1].sup.+ Compound 25 Ethyl 4-(2-cyano-4-(N-(2-fluoro benzyl)propanesulfonamido)phenyl) piperazin-l-formate [00037] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.72(m, 1H), 7.63(dd, J = 8.0, 7.5 Hz, 1H), 7.26(dd, J = 7.5, 5.0 Hz, 1H), 7.10(m, 1H), 6.82(d, J = 7.5 Hz 1H), 6.77(d, J = 7.5 Hz), 6.75(m, 1H), 4.56(s, 1H), 4.13(q, J = 7.8 Hz, 2H), 3.32-3.30 (m, 8H), 3.11(d, J = 7.1 Hz, 2H), 1.72(m, 2H), 1.25(t, J = 7.5 Hz, 3H), 1.00 (t, J = 7.2 Hz, 3H); ESI-MS: m/z = 489 [M + 1].sup.+ Compound 26 Ethyl 4-(2-cyano-4-(N-(3-methoxy benzyl)propanesulfonamido)phenyl) piperazin-l-formate [00038] .sup.1IHNMR (500 MHz, Chloroform-d): δ 7.25(m, 1H), 6.99-6.97(m, 3H), 6.81(d, J = 7.5 Hz 1H), 6.77 (d, J = 7.5 Hz), 6.74(m, 1H), 4.56(s, 2H), 4.12(q, J = 7.9 Hz, 2H), 3.70(s, 3H), 3.32-3.30 (m, 8H), 3.12(t, J = 7.2 Hz, 2H), 1.68(m, 2H), 1.21(t, J = 7.9 Hz, 3H), 0.98 (t, J = 7.8 Hz, 3H); ESI-MS: m/z = 501 [M + 1].sup.+ Compound 27 Ethyl 4-(2-cyano-4-(N-(4- methoxybenzyl)propanesulfonamido) phenyl)piperazin-1-formate [00039] .sup.1HNMR (500 MHz,Chloroform-d): δ 7.13(d, J = 7.5 Hz, 2H), 6.89(d, J = 7.5 Hz, 2H), 6.82(d, J = 7.5 Hz 1H), 6.78 (d, J = 7.5 Hz, 1H), 6.74(s, 1H), 4.56(s, 2H), 4.12(q, J = 7.8 Hz, 2H), 3.81(s, 3H), 3.32-3.29 (m, 8H), 3.11(t, J = 7.2 Hz, 2H), 1.68(m, 2H), 1.22(t, J = 8.0 Hz, 3H), 0.99 (t, J = 8.0 Hz, 3H); ESI-MS: m/z = 531 [M + 1].sup.+ Compound 28 Ethyl 4-(2-cyano-4-(N-(3,4- dimethoxybenzyl)propanesulfonamido) phenyl)piperazin-1-formate [00040] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.28(d, J = 7.5 Hz, 1H), 6.81(d, J = 7.5 Hz, 1H), 6.77(d, J = 7.5 Hz 1H), 6.75 (d, J = 7.5 Hz, 1H), 6.61(s, 1H), 6.57(d, J = 7.5 Hz, 1H), 4.55(s, 2H), 4.14(q, J = 7.9 Hz, 2H), 3.84(s, 3H), 3.72(s, 3H), 3.32-3.29 (m, 8H), 3.10(t, J = 7.2 Hz, 2H), 1.68(m, 2H), 1.23(t, J = 7.9 Hz, 3H), 0.98(t, J = 7.9 Hz, 3H); ESI-MS: m/z = 531 [M + 1].sup.+ Compound 29 Ethyl 4-(2-cyano-4-(N-(2-fluoro-4- methoxybenzyl)propanesulfonamido) phenyl)piperazin-1-formate [00041] .sup.1HNMR (500 MHz, Chloroform-d) δ 7.26(dd, J = 7.5,5.0 Hz, 1H), 7.16(dd, J = 8.0,1.5 Hz, 1H), 6.81(d, J = 7.5 Hz 1H), 6.77(d, J = 7.5 Hz, 1H), 6.75(s, 1H), 6.66(dd, J = 7.5, 1.5 Hz, 1H), 4.58(s, 2H), 4.11(q, J = 7.8 Hz, 2H), 3.81(s, 3H), 3.33-3.29 (m, 8H), 3.10(t, J = 7.2 Hz, 2H), 1.68(m, 2H), 1.22(t, J = 8.0 Hz, 3H), 0.97 (t, J = 8.0 Hz, 3H); ESI-MS: m/z = 519[M + 1].sup.+ Compound 30 Ethyl 4-(2-cyano-4-(N-(4-fluoro-2- methoxybenzyl)propanesulfonamido) phenyl)piperazin-1-formate [00042] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.28(dd, J = 7.5, 5.0 Hz, 1H), 7.17(dd, J = 8.0, 1.5 Hz, 1H), 6.87(m, 1H), 6.82(d, J = 7.5 Hz 1H), 6.77(d, J = 7.5 Hz, 1H), 6.74(s, 1H), 4.60(s, 2H), 4.12(q, J = 8.0 Hz, 2H), 3.72(s, 3H), 3.32-3.29 (m, 8H), 3.13(t, J = 7.2 Hz, 2H), 1.69(m, 2H), 1.26(t, J = 8.0 Hz, 3H), 0.98 (t, J = 8.0 Hz, 3H); ESI-MS: m/z = 519[M + 1].sup.+ Compound 31 Ethyl 4-(2-cyano-4-(N-(pyrimidin-4- ylmethyl)propanesulfonamido)phenyl) piperazin-l-formate [00043] .sup.1HNMR (500 MHz, Chloroform-d): δ 9.12(s, 1H), 8.95(d, J = 7.4 Hz, 1H), 7.21(d, J = 7.4 Hz, 1H), 6.85(d, J = 7.5 Hz 1H), 6.79(d, J = 7.5 Hz, 1H), 6.75(s, 1H), 4.63(s, 2H), 4.12(q, J = 7.4 Hz, 2H), 3.72(s, 3H), 3.32-3.29 (m, 8H), 3.12(t, J = 7.2 Hz, 2H), 1.70(m, 2H), 1.26(t, J = 7.1 Hz, 3H), 0.97 (t, J = 7.1 Hz, 3H); ESI-MS: m/z = 473 [M + 1].sup.+ Compound 32 Ethyl 4-(2-cyano-4-(N-(pyridin-2- ylmethyl)propanesulfonamido)phenyl) piperazin-l-formate [00044] .sup.1HNMR (500 MHz, Chloroform-d): δ 8.51 (d, J = 7.4 Hz, 1H), 7.74(m, 1H), 7.31(d, J = 7.4 Hz, 1H), 7.24(m, 1H), 6.88(d, J = 7.5 Hz 1H), 6.81(d, J = 7.5 Hz, 1H), 6.80(s, 1H), 4.62(s, 2H), 4.78(q, J = 7.4 Hz, 2H), 3.52-3.39 (m, 8H), 3.12(t, J = 7.2 Hz, 2H), 1.73(m, 2H), 1.26(t, J = 7.1 Hz, 3H), 1.01 (t, J = 7.1 Hz, 3H); ESI-MS: m/z = 472 [M + 1].sup.+ Compound 33 Ethyl 4-(2-cyano-4-(N-(furan-2- ylmethyl)propanesulfonamido)phenyl) piperazin-l-formate [00045] .sup.1HNMR (500 MHz,Chloroform-d): δ 7.55(d, J = 7.4 Hz, 1H), 6.88(d, J = 7.5 Hz 1H), 6.78(d, J = 7.5 Hz, 1H), 6.75(s, 1H), 6.39(m, 1H), 6.29(d, J = 7.2 Hz), 4.53(s, 2H), 4.15(q, J = 7.4 Hz, 2H), 3.35 = 3.30 (m, 8H), 3.12(t, J = 7.2 Hz, 2H), 1.70(m, 2H), 1.26(t, J = 7.1 Hz, 3H), 0.99 (t, J = 7.1 Hz, 3H); ESI-MS: m/z = 461 [M + 1].sup.+ Compound 34 Ethyl 4-(2-cyano-4-(N-(thiazol-4- ylmethyl)propanesulfonamido)phenyl) piperazin-l-formate [00046] .sup.1HNMR (500 MHz, Chloroform-d): δ 9.07(s,1H), 6.89(s, 1H), 6.88(d, J = 7.5 Hz 1H), 6.78(d, J = 7.5 Hz, 1H), 6.75(s, 1H), 4.65(s, 2H), 4.17(q, J = 7.4 Hz, 2H), 3.36-3.31 (m, 8H), 3.10(t, J = 7.2 Hz, 2H), 1.69(m, 2H), 1.22(t, J = 7.1 Hz, 3H), 0.98 (t, J = 7.1 Hz, 3H); ESI-MS: m/z = 478[+M + 1].sup.+ Compound 35 Ethyl 4-(2-cyano-4-(N-((1-methyl- 1H-imidazol-5-yl)methyl)propanesulf- onamido)phenyl)piperazin-l-formate [00047] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.62(s,1H), 7.18(s, 1H), 6.89(d, J = 7.5 Hz 1H), 6.80(d, J = 7.5 Hz, 1H), 6.77(s, 1H), 4.68(s, 2H), 4.14(q, J = 7.4 Hz, 2H), 3.72(s, 3H), 3.35- 3.30 (m, 8H), 3.13(t, J = 7.2 Hz, 2H), 1.69(m, 2H), 1.24(t, J = 7.2 Hz, 3H), 1.01 (t, J = 7.2 Hz, 3H); ESI-MS: m/z = 475[M + 1].sup.+ Compound 36 Ethyl 4-(2-cyano-4-(N-((1-methyl- 1H-pyrazol-5-yemethyppropanesulfon- amido)phenyl)piperazin-l-formate [00048] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.31(d, J − 7.4 Hz, 1H), 6.86(d, J = 7.5 Hz 1H), 6.81 (d, J = 7.5 Hz, 1H), 6.78(s, 1H), 4.63(s, 2H), 4.15(q, J = 7.4 Hz, 2H), 3.63(s, 3H), 3.32- 3.29 (m, 8H), 3.11 (t, J = 7.2 Hz, 2H), 1.70 (m, 2H), 1.21(t, J = 7.2 Hz, 3H), 1.00 (t, J = 7.2 Hz, 3H); ESI-MS: m/z = 475[M + 1].sup.+

Preparation Example 23. Synthesis of compounds 37-48

[0181] ##STR00049## [0182] 37a, 37b, 37: R.sub.1=N,N-dimethylformamido R.sub.2=4-fluorophenyl, R.sub.3=2methylpropyl [0183] 38a, 38b, 38: R.sub.1=N,N-dimethylformamido, R.sub.2=4-fluorophenyl, R.sub.3=n-butyl [0184] 39a, 39b, 39: R.sub.1=N,N-dimethylformamido, R.sub.2=4-fluorophenyl, R.sub.3=3-methylbutyl [0185] 40a, 40b, 40: R.sub.1=N,N-dimethylformamido, R.sub.2=4-fluorophenyl, R.sub.3=2,2-dimethylpropyl [0186] 41a, 41b, 41: R.sub.1=N,N-dimethylformamido, R.sub.2=pyridin-4-yl, R.sub.3=n-propyl [0187] 42a, 42b, 42: R.sub.1=N,N-dimethylformamido, R.sub.2=pyrimidin-4-yl, R.sub.3=n-propyl [0188] 43a, 43b, 43: R.sub.1=N,N-dimethylformamido, R.sub.2=4-fluorophenyl, R.sub.3=n-propyl [0189] 44a, 44b, 44: R.sub.1=N,N-dimethylformamido, R.sub.2=4-chlorophenyl, R.sub.3=n-propyl [0190] 45a, 45b, 45: R.sub.1=N,N-dimethylformamido, R.sub.2=4-methoxyphenyl, R.sub.3=n-propyl [0191] 46a, 46b, 46: R.sub.1=N,N-dimethylformamido, R.sub.2=1-methylpyrazol-5-yl, R.sub.3=4-fluorophenyl [0192] 47a, 47b, 47: R.sub.1=N,N-dimethylformamido, R.sub.2=2,4-difluorophenyl, R.sub.3=cyclopropylmethyl [0193] 48a, 48b, 48: R.sub.1=N,N-dimethylformamido, R.sub.2=4-fluorophenyl, R.sub.3=pyridin-3-yl

Step 1. Synthesis of intermediates 37a-48a

[0194] The synthesis was accomplished with reference to Steps 1-2 of Example 1, except that 2-chloro-5-nitrobenzonitrile and corresponding monosubstituted piperazine were used as raw materials to prepare compound 37a-48a via substitution and nitro reduction.

Step 2. Synthesis of compounds 37b-48b

[0195] The synthesis was accomplished with reference to Step 3 of Example 1, except that 37a-48a and corresponding aromatic aldehyde were used as raw materials to prepare compounds 37b-48b.

Step 3. Synthesis of compounds 37-48

[0196] The synthesis was accomplished with reference to Step 4 of Example 1, except that 37b-48b and corresponding sulfonyl chloride were used as raw materials to prepare compounds 37-48.

TABLE-US-00002 TABLE 1.2 NMR and MS data of compounds 37-48 Preparation Example Compound No. Name and structure of compound .sup.1HNMR and MS(ESI) Compound 37 4-(2-cyano-4-((N-(4-fluorobenzyl)- 2-methylpropyl)sulfonamido) pheny1)-N,N-dimethylpiperazin-1- formamide [00050] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.56(dd, J = 7.5, 5.0 Hz, 2H), 7.21(dd, J = 8.0, 7.5 Hz, 2H), 6.81-6.74(m, 3H), 4.67(s, 2H), 3.52(m, 4H), 3.33-3.29 (m, 6H), 2.98(s, 6H), 1.72(m, 1H), 0.93(d, J = 6.8 Hz, 6H); ESI-MS: m/z = 502[M + 1].sup.+ Compound 38 4-(2-cyano-4-(N-(4-fluorobenzyl) butanesulfonamido)pheny1)-N,N- dimethylpiperazin-l-formamide [00051] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.65(ddd, J = 7.5, 4.8, 1.5 Hz, 2H), 7.23(ddd, J = 8.0, 7.4, 1.5 Hz, 2H), 7.02(d, J = 7.5 Hz, 1H), 6.88(d, J = 7.5 Hz, 1H), 6.75(s, 1H), 4.62(s, 2H), 3.53(m, 4H), 3.30(m, 4H), 3.15(t, J = 7.2 Hz, 2H), 2.99(s, 6H), 1.61(m, 2H), 1.30(m, 2H), 0.95(t, J = 7.2 Hz, 3H); ESI-MS: m/z = 502[M + 1].sup.+ Compound 39 4-(2-cyano-4-((N-(4-fluorobenzyl)- 3-methylbutyl)sulfonamido) pheny1)-N,N-dimethylpiperazin-1- formamide [00052] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.67(ddd, J = 7.5, 5.0, 1.5 Hz, 2H), 7.25(ddd, J = 8.0, 7.5, 1.5 Hz, 2H), 7.00(d, J = 7.5 Hz, 1H), 6.86(d, J = 7.5 Hz, 1H), 6.78(s, 1H), 4.61(s, 2H), 3.51(m, 4H), 3.32(m, 4H), 3.13(t, J = 7.1 Hz, 2H), 3.00(s, 6H), 1.77(m, 2H), 1.62(m, 1H), 0.95(d, J = 7.1 Hz, 6H); ESI-MS: m/z = 516[M + ].sup.+ Compound 40 4-(2-cyano-4-((N-(4-fluorobenzyl)- 2,2-dimethylpropyl)sulfonamido) phenyl)-N,N-dimethylpiperazin- 1-formamide [00053] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.69(ddd, J = 7.5, 5.0, 1.5 Hz, 2H), 7.27(ddd, J = 8.0, 7.5, 1.5 Hz, 2H), 7.05(d, J = 7.5 Hz, 1H), 6.90(d, J = 7.5 Hz, 1H), 4.67(s, 2H), 3.52(m, 4H), 3.30(m, 4H), 3.27(s, 2H), 2.99(s, 6H), 0.96(s, 9H); ESI-MS: m/z = 526[M + 1].sup.+ Compound 41 4-(2-cyano-4-(N-(pyridin-4-yl- methyl)propanesulfonamido)pheny1)- N,N-dimethylpiperazin-l-formamide [00054] .sup.1HNMR (500 MHz, Chloroform-d): δ 6 8.55(d, J = 7.5 Hz, 2H), 7.35(d, J = 7.5 Hz, 2H), 6.91(d, J = 7.5 Hz, 1H), 6.80(d, J = 7.5 Hz, 1H), 6.78(s, 1H), 5.10(s, 2H), 3.52(m, 4H), 3.30(m, 4H), 3.12(t, J = 7.1 Hz, 2H), 2.99(s, 6H), 1.69(m, 2H), 0.98(t, J = 7.1 Hz, 3H); ESI-MS: m/z = 471[M + 1].sup.+ Compound 42 4-(2-cyano-4-(N-(pyrimidin-4-y1 methyl)propanesulfonamido) pheny1)-N,N-dimethylpiperazin-1- formamide [00055] .sup.1HNMR (500 MHz,Chloroform-d): δ 9.12(s, H), 8.95 (d, J = 7.5 Hz, 1H), 7.25(d, J = 7.5 Hz, 1H), 7.00(d, J = 7.5 Hz, 1H), 6.87(d, J = 7.5 Hz, 1H), 6.77(s, 1H), 458(s, 2H), 3.55(m, 4H), 3.31(m, 4H), 3.13(t, J = 7.1 Hz, 2H), 2.99(s, 6H), 1.69(m, 2H), 0.99(t, J = 7.1 Hz, 3H); ESI-MS: m/z = 472[M + 1].sup.+ Compound 43 4-(2-cyano-4-(N-(4-fluorobenzyl) ethanesulfonamido)pheny1)-N,N- dimethylpiperazin-l-sulfonamide [00056] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.62(ddd, J = 7.5, 5.0, 1.5 Hz, 2H), 7.21(ddd, J +32 8.0, 7.5, 1.5 Hz, 2H), 7.00(d, J = 7.5 Hz, 1H), 6.83(d, J = 7.5 Hz, 1H), 6.73(s, 1H), 4.62(s, 2H)3.45(q, J = 7.1 Hz, 2H), 3.19(m, 4H), 2.68(s, 6H), 2.58(m, 4H), 1.27(t, J = 7.1 Hz, 3H); ESI-MS: m/z = 510[M + 1].sup.+ Compound 44 4-(4-(N-(4-chlorobenzyl)propanesulf- onamido)-2-cyanopheny1)-N,N- dimethylpiperazin-l-sulfonamide [00057] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.45(d, J = 7.5 Hz, 2H), 7.35(d, J = 7.5 Hz, 2H), 6.90(d, J = 7.5 Hz, 1H), 6.81(d, J = 7.5 Hz, 1H), 6.77(s, 1H), 4.63(s, 2H), 3.20(m, 4H), 3.12(t, J = 7.1 Hz, 2H), 2.67(s, 6H), 2.58(m, 4H), 1.70(m, 2H), 1.00(t, J = 7.1 Hz, 3H); ESI-MS: m/z = 541[M + 1].sup.+ Compound 45 4-(2-cyano-4-(N-(4-methoxybenzyl) propanesulfonamido)pheny1)-N, N-dimethylpiperazin-l-sulfonamide [00058] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.22(d, J = 7.5 Hz, 2H), 6.99(d, J = 7.5 Hz, 2H), 6.88 (d, J= 7.5Hz, 1H), 6.79(d, J = 7.5 Hz, 1H), 6.75(s, 1H), 4.62(s, 2H), 3.81(s, 3H), 3.20(m, 2H), 3.10(t, J = 7.1 Hz, 2H), 2.67(s, 6H), 2.58(m, 4H), 1.69(m, 2H), 1.01(t, J = 7.1 Hz, 3H); ESI-MS: m/z = 536[M + 1].sup.+ Compound 46 4-(2-cyano-4-((4-fluoro-N-((1- methy1-1H-pyrazol-5-y)methyl)phenyl) sulfonamido)pheny1)-N,N- dimethylpiperazin-1-sulfonamide [00059] .sup.1HNMR (500 MHz, Chloroform-d): δ 8.00(ddd, J = 7.5, 5.0, 1.5 Hz, 2H), 7.45(ddd, J = 8.0, 7.5, 1.5 Hz, 2H), 7.21(d, J = 7.5 Hz, 1H), 6.98(d, J = 7.5 Hz, 1H), 6.80(d, J = 7.5 Hz, 1H), 6.76(s, 1H), 6.05(d, J = 7.5 Hz, 1H), 4.63(s, 2H), 3.63(s, 1H), 3.20(m, 4H), 2.66(s, 6H), 2.58(m, 4H); ESI-MS: m/z = 562[M + 1].sup.+ Compound 47 4-(2-cyano-4((1-cyclopropyl-N-( 2,4-difluorobenzyl)methyl)sulfon- amido)phenyyl-N,N-dimethylpipe- razin-l-sulfonamide [00060] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.30(dd, J = 7.5, 5.0 Hz,1H), 6.96(ddd, J = 8.0,7.5, 1.5 Hz, 1H), 6.81(d, J = 7.5 Hz, 1H), 6.77-6.71(m, 3H), 4.60(s, 2H), 3.35(d, J = 7.1 Hz, 2H), 3.20(m, 4H), 2.66(s, 6H), 2.57(m, 4H), 1.02(m, 1H), 0.80-0.40(m, 4H); ESI-MS: m/z = 554[M + 1].sup.+ Compound 48 4-(2-cyano-4-(-N-(4-fuorophenyl) pyridin-3-sulfonamido)pheny1)-N, N-dimethylpiperazin-l-sulfonamide [00061] .sup.1HNMR (500 MHz, Chloroform-d): δ 8.95(s, 1H), 8.88(d, J = 7.5, 5.0 Hz, 7H), 8.46(d, J = 7.4H, 1H), 7.63(m, 2H), 7.38(dd, J = 7.5, 5.0 Hz, 2H), 7.16(dd, J = 8.0, 7.5 Hz, 2H), 6.85(d, J = 7.5 Hz, 1H), 6.79(d, J = 7.5 Hz, 1H), 6.76(s, 1H), 4.65(s, 1H), 3.22(m, 4H), 2.66(s, 6H), 2.58(m, 4H); ESI-MS: m/z = 559[M + 1].sup.+

Preparation Example 24. Synthesis of ethyl 4-(4-(N-(4-fluorophenyl) propanesulfonamido)-2-(trifluoromethyl)phenyl)piperazin-1-formate (compound 49)

[0197] ##STR00062##

[0198] The synthesis was accomplished with reference to Steps 1-4 of Example 1, except that 1-chloro-4-nitro-2-(trifluoromethyl)benzene and corresponding monosubstituted piperazine were used as raw materials to prepare compound 49 via substitution, nitro reduction, reductive amination and sulfonamidation. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.64-7.61 (m, 2H), 7.25 (dd, J=8.8, 2.6 Hz, 2H), 6.87 (s, 1H), 6.56 (d, J=7.5 Hz, 1H), 6.49 (d, J=7.4 Hz, 1H), 4.63 (s, 2H), 4.15 (q, J=7.1 Hz, 2H), 3.32-3.29 (m, 8H), 3.15 (t, J=7.1 Hz, 2H), 1.70 (m, 2H), 1.30 (t, J=7.1 Hz, 3H), 0.91 (t, J=7.4 Hz, 3H); ESI-MS: m/z=532[M+1].sup.+.

Preparation Example 25. Synthesis of Compounds 50-58

[0199] ##STR00063##

[0200] Compounds 50-58 were prepared using parallel synthesis method well-known to those skilled in the art. The starting material, N-(3-cyano-4-fluorophenyl)-N-(4-fluorobenzyl)propanesulfonamide, was prepared with reference to the synthesis procedure of 5b in Steps 1 and 2 of Preparation Example 5.

Step 1. Synthesis of intermediates 50b-52b

[0201] 50a-52a (2.5 mmol) were placed into three parallel reaction flasks, respectively, and were dissolved with anhydrous DMF. The resultant mixtures were each cooled to 0° C., to which 60% sodium hydride (0.12 g, 3.0 mmol) was added, and stirred for 0.5 h with the temperature maintained, and to which N-(3-cyano-4-fluorophenyl)-N-(4-fluorobenzyl)propanesulfonamide (1.0 g, 2.7 mmol) was further added, warmed to room temperature, and allowed for reaction for 1 h. After completion of the reaction, the reaction mixtures were each poured into water, extracted with ethyl acetate, washed with saturated saline, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residues were each purified with silica gel column chromatography (PE:EA=2:1, v/v), to give 50b-52b.

Step 2. Synthesis of compounds 50-52

[0202] Intermediates 50b-52b (1.5 mmol) were dissolved in 30 mL of DCM, respectively, to which trifluoroacetic acid (5.0 g, 43.1 mmol) was added, followed by stirring at room temperature for 1 h. The resultant mixtures were each alkalized with a sodium carbonate solution, extracted with dichloromethane, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residues were each purified with silica gel column chromatography (DCM:MeOH=20:1, v/v), to give compounds 50-52.

Step 3. Synthesis of compounds 53-55

[0203] 50-52 (0.45 mmol) were dissolved in 5 mL of DCM, respectively, to which triethylamine (0.05 g, 0.49 mmol) and then acetyl chloride (0.053 g, 0.67 mmol) were added sequentially, followed by stirring at room temperature for 1 h, and then concentration under reduced pressure. The residues were each purified with silica gel column chromatography (DCM:MeOH=20:1, v/v), to give compounds 53-55.

Step 4. Synthesis of compounds 56-58

[0204] N-formyl saccharin (0.047 g, 0.22 mmol) was added to 1 mL of THF, to which 50-52 (0.22 mmol) were then added, respectively, followed by stirring at room temperature for 15 min. The resultant mixtures were each alkalized with a sodium carbonate solution, extracted with ethyl acetate, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residues were each purified with silica gel column chromatography (DCM:MeOH=20:1, v/v), to give compounds 56-58.

Preparation Example 26. Synthesis of compounds 59 and 60

[0205] The synthesis was accomplished with reference to Steps 1-4 of Example 25, except that 5b was used as raw material to prepare compounds 59 and 60 via substitution, deprotection of Boc, and N-acetylation/formylation.

TABLE-US-00003 TABLE 1.3 NMR and MS data of Compounds 50-60 Preparation Example compound No. Name of compound NMR and MS data Compound 50 N-(3-cyano-4-(piperidin-4-yloxy) phenyl)-N-(4-fluorobenzyl) propanesulfonamide [00064] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.63(dd, J = 7.5, 5.0 Hz, 2H), 7.32(d, J = 7.4 Hz, 1H), 7.25 (ddd, J = 8.0, 7.5, 1.5 Hz, 2H), 7.04(d, J = 7.4 Hz, 1H), 6.95(s, 1H), 4.56(s, 2H), 4.23(br, 1H), 3.70(m, 1H), 3.12(t, J = 7.2 Hz, 2H), 2.80-2.69(m, 4H), 2.00-1.75 (m, 4H), 1.68(m, 2H), 0.97(t, J = 7.2 Hz, 3H); ESI-MS: m/z = 432[M + 1].sup.+ Compound 51 N-(3-cyano-4-(piperidin-4-ylthio) phenyl)-N-(4-fluorobenzyl) propanesulfonamide [00065] .sup.11-1NMR (500 MHz, Chloroform-d): g 7.66(dd, J = 7.5, 5.0 Hz, 2H), 7.44(d, J = 7.5 Hz, 1H), 7.23(dd, J = 8.0, 7.5 Hz, 2H), 6.85-6.82(m, 2H), 4.63(s, 2H), 4.15(br, 1H), 3.11(t, J = 7.2 Hz, 2H), 2.89(m, 1H), 2.81-2.70(m, 4H), 1.99-1.74(m, 4H), 1.68(m, 1H), 1.01(t, J = 7.2 Hz, 3H); ESI-MS: m/z = 478[M + 1].sup.+ Compound 52 N-(3-cyano-4-(piperidin-4-ylamino) pheny1)-N-(4-fluorobenzyl) propanesulfonamide [00066] .sup.1HNMR (500MHz, Chloroform-d): δ 7.56(dd, J = 7.5, 5.0 Hz, 2H), 7.22(dd, J = 8.0, 7.5 Hz, 2H), 6.85-6.82(m, 2H), 6.75(d, J = 7.5 Hz, 1H), 4.60(br, 1H), 4.53(s, 2H), 3.55(br, 1H), 3.10(t, J = 7.1 Hz, 2H), 2.79-2.63(m, 5H), 1.81-1.56(m, 6H), 0.97(t, J = 7.1 Hz, 3H),; ESI-MS: m/z = 431[M + 1].sup.+ Compound 53 N-(4((1-acetylpiperidin-4-ypoxy)- 3-cyanopheny1)-N-(4-fluorobenzyl) propanesulfonamide [00067] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.63(dd, J = 7.5, 5.0 Hz, 2H), 7.38(d, J = 7.5 Hz, 1H), 7.22(dd, J = 8.0, 7.5 Hz, 2H), 7.04(d, J = 7.5 Hz, 1H), 6.92(s, 1H), 4.67(s, 2H), 3.76(m, 1H), 3.59-3.49(m, 4H), 3.11(t, J = 7.2 Hz, 2H), 2.15-1.90(m, 4H), 2.10(s, 3H), 1.68(m, 2H), 0.98(t, J = 7.2 Hz, 3H); ESI-MS: m/z = 474[M + 1].sup.+ Compound 54 N-(4-((1-acetylpiperidin-4-yl)thio)- 3-cyanophenyI)-N-(4-fluorobenzyl) propanesulfonamide [00068] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.65(dd, J = 7.5, 5.0 Hz, 2H), 7.44(d, J = 7.5 Hz, 1H), 7.21(dd, J = 8.0, 7.5 Hz, 2H), 6.88(d, J = 7.5 Hz, 1H), 6.82(s, 1H), 4.66(s, 2H), 3.59-3.49(m, 4H), 3.11(t, J = 7.2 Hz, 2H), 2.94(m, 1H), 2.13-1.88(m, 4H), 2.11(s, 3H), 1.69(m, 2H), 1.69(m, 2H), 1.00(t, J = 7.2 Hz, 3H); ESI-MS: m/z = 490[M + 1].sup.+ Compound 55 N-(4((1-acetylpiperidin-4-yl) ami-no)-3-cyanophenyI)-N-(4- fluorobenzyl)propanesulfonamide [00069] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.60(dd, J = 7.5, 5.0 Hz, 2H), 7.23(dd, J = 8.0,7.5 Hz, 2H), 6.85-6.82(m, 2H), 6.75(d, J = 7.5 Hz, 1H), 4.71 (br, 1H), 4.62(s, 2H), 3.60-3.50(m, 4H), 3.09(t, J = 7.1 Hz, 2H), 2.68(m, 1H), 2.10(s, 3H), 1.97-1.72 (m, 6H), 0.98(t, J = 7.2 Hz, 3H); ESI-MS: m/z = 473[M + 1].sup.+ Compound 56 N-(4-((1-formylpiperidin-4-yl)oxy)- 3-cyanophenye-N-(4-fluorobenzyl) propanesulfonamide [00070] .sup.1HNMR (500 MHz, Chloroform-d): δ 8.05(s, 1H), 7.59(dd, J = 7.5, 5.0 Hz, 2H), 7.32(d, J = 7.5 Hz, 1H), 7.22(dd, J = 8.0, 7.5 Hz, 2H), 4.62(s, 2H), 3.76(m, 1H), 3.59-3.48(m, 4H), 3.10(t, J = 7.1 Hz, 2H), 2.15-1.90(m, 4H), 1.68(m, 1H), 0.96(t, J = 7.1 Hz, 3H); ESI-MS: m/z = 460[M + 1].sup.+ Compound 57 N-(4((1-formylpiperidin-4-yl)-thio)- 3-cyanophenye-N-(4-fluorobenzyl) propanesulfonamide [00071] .sup.1HNMR (500 MHz, Chloroform-d): δ 8.04(s, 1H), 7.63(dd, J = 7.5, 5.0 Hz, 2H), 7.44(d, J = 7.5 Hz, 1H), 7.38(dd, J = 8.0, 7.5 Hz, 2H), 6.85-6.83(m, 2H), 4.66(s, 2H), 3.60-3.51(m, 4H), 3.12(t, J = 7.2 Hz, 2H), 2.95(m, 1H), 2.14-1.89(m, 4H), 1.70(m, 2H), 1.00(t, J = 7.2 Hz, 3H); ESI-MS: m/z = 476[M + 1].sup.+ Compound 58 N-(4((1-formylpiperidin-4-yl) amino)-3-cyanophenye-N-(4-fluoro- benzyl)propanesulfonamide [00072] .sup.1HNMR (500 MHz, Chloroform-d): δ 8.08(s, 1H), 7.65(dd, J = 7.5, 4.9 Hz, 2H), 7.25(dd, J = 8.0, 7.5 Hz, 2H), 6.84-6.83(m, 2H), 6.75(d, J = 7.5 Hz, 1H), 4.67(s, 2H), 4.55(br, 1H), 3.59-3.50(m, 4H), 3.12(t, J = 7.1 Hz, 2H), 2.68(m, 1H), 1.97-1.73(m, 6H), 0.95(t, J = 7.1 Hz, 3H); ESI-MS: m/z = 459[M + 1].sup.+ Compound 59 N-(4((1-acetylpiperidin-4-yl)oxy)- 3-cyanopheny1)-N-benzylbenze nesulfonamide [00073] .sup.1HNMR (500 MHz, Chloroform-d): δ 7.71- 7.65 (m, 3H), 7.57 (t, J =7.8 Hz, 2H), 7.27- 7.18 (m, 6H), 7.05 (d, J = 2.6 Hz, 1H), 6.81 (d, J = 9.1 Hz, 1H), 4.68 (s, 2H), 4.66-4.61 (m, 1H), 3.91-3.81 (m, 1H), 3.75-3.67 (m, 1H), 3.64-3.54 (m, 1H), 3.54-3.43 (m, 1H), 2.13 (s, 3H), 1.94-1.83 (m, 4H); ESI-MS: m/z = 490[M + 1].sup.+ Compound 60 N-(4((1-formylpiperidin-4-yl)oxy)- 3-cyanophenye-N-benzylbenzenesulfonamide [00074] .sup.1H NMR (500 MHz, Chloroform-d) : δ 8.06 (s, 1H), 7.71-7.65 (m, 3H), 7.57 (t, J = 7.8 Hz, 2H), 7.28-7.22 (m, 4H), 7.22-7.19 (m, 2H), 7.06 (d, J = 2.6 Hz, 1H), 6.82 (d, J = 9.1 Hz, 1H), 4.72-4.69 (m, 1H), 4.68 (s, 2H), 3.89-3.76 (m, 1H), 3.69-3.61 (m, 1H), 3.58-3.48 (m, 1H), 3.43-3.36 (m, 1H), 1.99-1.84 (m, 4H); ESI-MS: m/z = 476[M + 1].sup.+

Preparation Example 27. Synthesis of ethyl 4-(4-(N-benzylpropane sulfonamido)2-cyanophenyl)-3-oxopiperazin-1-formate (compound 61)

[0206] ##STR00075##

Step 1. Synthesis of intermediate N-benzyl-N-(4-bromo-3-cyanophenyl) propanesulfonamide (61a)

[0207] The synthesis was accomplished with reference to Step 2 of Example 17, except that 17a and propane sulfonyl chloride were used as raw materials to prepare compound 61a, ESI-MS: m/z=394[M+1].sup.+.

Step 2. Synthesis of ethyl 4-(4-(N-benzylpropanesulfonamido)2-cyano phenyl)-3-oxopiperazin-1-formate (compound 61)

[0208] The synthesis was accomplished with reference to Step 3 of Example 17, except that 61a and ethyl 3-oxopiperazin-1-formate were used as raw materials to prepare compound 61. .sup.1H NMR (500 MHz, Chloroform-d): δ 7.43 (d, J=7.5 Hz, 2H), 7.31-7.29 (m, 3H), 7.25 (d, J=7.5 Hz, 2H), 7.21 (d, J=7.5 Hz, 1H), 6.97 (s, 1H), 4.65 (s, 2H), 4.14 (q, J=7.1 Hz, 2H), 3.79 (s, 2H), 3.64 (t, J=7.1 Hz, 2H), 3.24 (t, J=7.1 Hz, 2H), 3.10 (t, J=7.1 Hz, 2H), 1.70 (m, 2H), 1.22 (t, J=7.2 Hz, 3H), 0.99 (t, J=7.2 Hz, 3H); ESI-MS: m/z=485[M+1].sup.+.

Example 28 Biological Assessments

I. IonWorks Automated Patch Clamp Assay of Inhibitory Activity Against Kv1.3

[0209] The principle and method for Kv1.3 IonWorks assay can be found in Schroeder et al. [Schroeder et al. J Biomol Screen 2003, 8(1):50-64]

[0210] 1. Cell Culture

[0211] Inhibitory activity of the compounds against Kv1.3 was assessed using a CHO-K1 recombinant cell line stably expressing human Kv1.3 (Charles River, Calif.). The cells were cultured in an F-12K culture medium containing 5% calf serum (Invitrogen, Carlsbad. Calif.) in an incubator at 37° C. and under air humidity with 6% CO.sub.2. Prior to be fed in the IonWorks system, the adherent cells were treated with Versene at 37° C. for 6-7 min. After gently beating the culture flask, the cells were re-suspended in a PBS phosphate buffer, and then were centrifuged at 50×g for 4 min. After a short grinding, the cells were eventually re-suspended in an external recording solution at a density of approximately 1×10.sup.6/mL.

[0212] 2. Preparation of Plates for Assay of Compounds

[0213] The compounds were dissolved in DMSO to formulate a final assay concentration of 300× on the master plate. The final gradient assay concentrations were 0.00384, 0.00192, 0.096, 0.048, 0.24, 1.2, 6 and 30 μM. All 300× stock solutions of each compound in DMSO were transferred to a 384-well plate with 2 μl per well. The plates were sealed and stored at −80° C. until the day of assay.

[0214] On the day of assay, the plates were each thawed at room temperature. After centrifugation, 198 μl of the external recording solution (with the components of: 130 mM Na-Gluconate, 20 mM NaCl, 4 mM KCl, 1 mM MgCl.sub.2, 1.8 mM CaCl.sub.2, 5 mM Glucose and 10 mM HEPES, pH7.3) was added and mixed thoroughly. This step provided a 1:100 dilution for the compounds. After addition of cells to IonWorks, a further 1:3 dilution was carried out, resulting in a total dilution of 1:300. At least 8 wells were reserved as blank control on each of the plates, i.e., containing only 0.3% DMSO. In addition, at least 8 wells were reserved as positive control to detect the specificity to cell signals. Fluoxetine, a compound as the positive control, was assayed at its maximum blocking concentration (100 μm) and secondary maximum blocking concentration (10 μm). In addition, an internal recording solution used in the assay (with the components of: 100 mM K-gluconate, 40 mM KCl, 1 mM MgCl.sub.2, 1 mM EGTA and 10 mM HEPES, pH7.3) contained Amphotericin B at a final concentration of 200 μg/mL to gain current channels inside the cells. The osmotic pressure of the solution was adjusted with sucrose.

[0215] 3. Experimental Procedures and Data Analysis

[0216] Experimental procedures were carried out following the protocols as set by IonWorks Quattro system (Molecular Devices, San Jose, Calif.). The current of human Kv1.3 was elicited to 0 millivolt (mV) for four times by a pulse lasting 1 second. A potential of −80 mV was maintained between pulses, for 5 seconds. First, a early voltage application program was started in the IonWorks Quattro system, and then the compounds were added and incubated for 600 seconds. Next, a late voltage application program was started until completion of all assays.

[0217] The maximum peak outward currents both before and after addition of the compounds as well as the average tail current elicited when the fourth pulse rose to 0 mV were measured. The inhibitory activity of the compounds against Kv1.3 can be calculated by dividing the measured current amplitude after addition of the compounds by the measured current amplitude before addition of the compounds.

[0218] All data was calibrated by a data filtration program of the IonWorks Quattro system. The filtration standard was that: seal quality >30 MΩ, decrease of seal resistance <50% and current amplitude >200 pA.

TABLE-US-00004 TABLE 2.1 The inhibitory activity of compounds against Kv1.3 (automated patch clamp method) Compound Kv1.3 No. (IC.sub.50, μM) 1 0.265 2 0.131 3 0.107 4 12.90 5 0.186 6 0.193 7 0.312 8 9.301 9 0.134 10 0.098 11 0.195 12 0.234 13 6.400 14 0.129 15 0.310 16 1.260 17 0.190 18 0.101 19 0.130 20 0.090 21 0.097 22 0.333 23 0.176 24 0.086 25 0.321 26 0.420 27 0.239 28 0.676 29 0.439 30 0.288 31 0.412 32 0.165 33 0.839 34 0.645 35 0.201 36 0.130 37 0.092 38 0.101 39 0.155 40 0.206 41 0.131 42 0.167 43 0.124 44 0.096 45 0.224 46 0.356 47 0.124 48 0.145 49 0.160 50 0.370 51 0.412 52 0.139 53 0.291 54 0.389 55 0.160 56 0.250 57 0.254 58 0.279 59 0.441 60 0.332 61 0.092

II. Conventional Patch Clamp Assay of Inhibitory Activity Against Kv1.3

[0219] In order to compare the influence of different assay methods on the inhibitory activity of a compound, the conventional patch clamp method was used to assay the inhibitory activities of compounds 21, 37, 44, 61 against Kv1.3. The principle and method for the conventional patch clamp method can be found in Grissmer et al. [Grissmer et al. Molecular Pharmacology 1994, 45:1227-1234].

[0220] 1. Cell Culture

[0221] Inhibitory activity of the compounds against Kv1.3 was assessed using a CHO-K1 recombinant cell line stably expressing human Kv1.3 (Charles River, Calif.). The cells were cultured in an F-12K culture medium containing 5% calf serum (Invitrogen, Carlsbad. Calif.) in an incubator at 37° C. and under air humidity with 6% CO.sub.2.

[0222] During the experiment, adherent cells were placed in a recording chamber under an inverted microscope. All the experiments were performed at room temperature. Each of the cells used itself as the control.

[0223] 2. Assay of the Compounds

[0224] The test compounds were formulated to their final concentrations on the day of assay, and then dissolved in an extracellular fluid. The extracellular fluid (mM) was: NaCl, 137; KCl, 4; CaCl.sub.2, 1.8; MgCl.sub.2, 1; HEPES, 10; glucose 10; pH 7.4 (NaOH titration). All the solutions of the test compounds and the control compounds contained 0.3% of DMSO.

[0225] Each of the compounds was perfused by a gravity-driven perfusion system. At least two cells were tested for each of the concentrations of a compound. After the current was stable, the blocking effect of the compounds was calculated by comparing the change in the current before and after administration of the compounds. 1000 μM 4-AP was used as a positive control.

[0226] 3. Electrophysiology

[0227] The cells were transferred to a perfusion chamber, and were perfused with the extracellular fluid. Intracellular fluid (mM): K-aspartate, 130; MgCl.sub.2, 5; EGTA 5; HEPES, 10; pH 7.2 (KOH titration). The intracellular fluid was stored in a small amount in a refrigerator at −80° C. in batches, and was thawed on the day of the experiment. The electrodes were drawn by PC-10 (Narishige, Japan). A whole-cell patch-clamp recording was performed, with the noise being filtered by one-fifth of the sampling frequency.

[0228] 4. Process of Assay and Analysis of Results

[0229] The cells were clamped at −80 mV and then depolarized to 0 mV with square waves lasting for 2 seconds to obtain a Kv1.3 current. This process was repeated every 20 seconds. The maximum current elicited by the square waves was detected. After the maximum current became stable, the test compounds were perfused. After the reaction was stable, the blocking strength was calculated.

[0230] Data was collected and analyzed using pCLAMP 10 (Molecular Devices, Union City, Calif.). A stable current meant a current which varies within a limited range over time. The value of the stable current was used to calculate the effect of a compound at this solubility.

TABLE-US-00005 TABLE 2.2 Inhibitory activity of certain compounds against Kv1.3 (conventional patch-clamp method) Compound Kv1.3 No. (IC.sub.50, μM) 21 0.141 37 0.108 44 0.111 61 0.130

III. Conventional Patch Clamp Assay of Inhibitory Activity Against Kv1.5

[0231] The principle and method for the conventional patch clamp method can be found in Grissmer et al. [Grissmer et al. Molecular Pharmacology 1994, 45:1227-1234].

[0232] 1. Cell Culture

[0233] Inhibitory activity of a compounds against Kv1.5 was assessed using a CHO-K1 recombinant cell line stably expressing human Kv1.5 (Charles River, Calif.). The cells were cultured in an F-12K culture medium containing 5% calf serum (Invitrogen, Carlsbad. Calif.) in an incubator at 37° C. and under air humidity with 6% CO.sub.2.

[0234] During the experiment, adherent cells were placed in a recording chamber under an inverted microscope. All the experiments were performed at room temperature. Each of the cells used itself as the control.

[0235] 2. Assay of the Compounds

[0236] The test compounds were formulated to their final concentrations on the day of assay, and then dissolved in an extracellular fluid. The extracellular fluid (mM) was: NaCl, 137; KCl, 4; CaCl.sub.2, 1.8; MgCl.sub.2, 1; HEPES, 10; glucose 10; pH 7.4 (NaOH titration). All the solutions of the test compounds and the control compounds contained 0.3% of DMSO.

[0237] Each of the compounds was perfused by a gravity-driven perfusion system. At least two cells were tested for each of the concentrations of a compound. After the current was stable, the blocking effect of the compounds was calculated by comparing the change in the current before and after administration of the compounds.

[0238] 1000 μM 4-AP was used as a positive control.

[0239] 3. Electrophysiology

[0240] The cells were transferred to a perfusion chamber, and were perfused with the extracellular fluid. Intracellular fluid (mM): K-aspartate, 130; MgCl.sub.2, 5; EGTA 5; HEPES, 10; pH 7.2 (KOH titration). The intracellular fluid was stored in a small amount in a refrigerator at −80° C. in batches, and was thawed on the day of the experiment. The electrodes were drawn by PC-10 (Narishige, Japan). A whole-cell patch-clamp recording was performed, with the noise being filtered by one-fifth of the sampling frequency.

[0241] 4. Process of Assay and Analysis of Results

[0242] The cells were clamped at −80 mV and then depolarized to 20 mV with square waves lasting for 2 seconds to obtain a Kv1.5 current. This process was repeated every 20 seconds. The maximum current elicited by the square waves was detected. After the maximum current became stable, the test compounds were perfused. After the reaction was stable, the blocking strength was calculated.

[0243] Data was collected and analyzed using pCLAMP 10 (Molecular Devices, Union City, Calif.). A stable current meant a current which varies within a limited range over time. The value of the stable current was used to calculate the effect of a compound at this solubility.

TABLE-US-00006 TABLE 2.2 Inhibitory activity of certain compounds against Kv1.5 (conventional patch-clamp method) Compound Kv1.5 No. (IC.sub.50, μM) 21 2.816 37 3.65 44 7.895 61 2.108

IV. Effects of Compound 37 on a Rat Model of Allergic Contact Dermatitis (ACD Model)

[0244] 1. Laboratory Animal

[0245] SPF SD female rats with body weight of 130-150 g were used.

[0246] 2. Drugs and Agents

[0247] Dexamethasone (DXMS) sodium phosphate injection, 5 mg/ml/injection (Shiyao Yinhu Pharmaceutical Co., Ltd.); 1-chloro-2,4-dinitrobenzene (DNCB) (TCI Shanghai); vehicle and negative control: acetone/DMSO 9:1 (Vehicle, Model group).

[0248] 3. Experimental Procedures

[0249] 3.1 The animals were randomly divided into 6 groups based on body weight with 10 animals per group: blank control group, model control group, positive control group (dexamethasone sodium phosphate injection 5 mg/mL), 5% Compound 37 group, 1.5% Compound 37 group and 0.5% Compound 37 group.

[0250] 3.2 Modeling and administration: a rat model of allergic contact dermatitis (ACD) was established by sensitizing and stimulating the rat's skin with a DNCB solution in acetone and DMSO (acetone-DMSO 9:1). The rats were normally fed in a SPF barrier system for 1 week. Long hair was removed with a razor and short hair was removed with depilatory cream within an area of about 2 cm×2 cm from abdomen of the rats one day before the experiment. On day 1 of the experiment, the depilation site was uniformly applied with 50 μL of 7% DNCB solution in acetone and DMSO for sensitization. On day 2 of the experiment, 50 μL of 1% DNCB solution in acetone and DMSO was used repeatedly to strengthen the sensitization. From day 5 of the experiment, for each of the rats, the right ear was stimulated by applying 20 μL of 1% DNCB solution in acetone and DMSO, and the left ear was stimulated by applying 20 μL of acetone-DMSO (acetone-DMSO 9:1) solution as the control; for the negative control group, the same site was only applied with 20 μL of acetone-DMSO solution (9:1) as the control, for 3 days. An ACD rat model induced by DNCB was established in rats with manifest erythema and swelling in ears. The drugs were administered to the right ear by wiping. The first administration was 6-8 h before stimulation. After the stimulation, the drugs were administered by wiping three times a day, with 25 μl each time, for 4 days. For the negative control group and the model control group, a blank medium of equal volume was administered to the right ear of the rats by wiping.

[0251] 3.3 Measurement of difference between ear thicknesses: before the stimulation and after the final administration, the thickness of central parts of left and right ears of the rats in each of the groups was measured with electronic digital calipers. The measurements were performed twice in parallel and averaged to calculate the difference between ear thicknesses (right ear thickness−left ear thickness).

[0252] 4. Conclusion: Compound 37 significantly reduced ear inflammation in rats with atopic dermatitis (ACD) in a dose-dependent manner. The experimental results were shown in FIG. 1.

V. Effects of Compound 24 on a Rat Model of Psoriasis Induced by Imiquimod

[0253] 1. Laboratory Animal

[0254] SPF Wista female rats with body weight of around 200 g were used.

[0255] 2. Drugs and Formulations

[0256] Dexamethasone (DXMS) sodium phosphate injection, 5 mg/ml/injection (Shiyao Yinhu Pharmaceutical Co., Ltd.); 5% imiquimod cream, 0.25 g/pack (Sichuan MED-SHINE Pharmaceutical Co., Ltd.); 3×106 U recombinant human interferon α-2a injection, 1.0 ml/vial (Shenyang Sunshine Pharmaceutical Co., Ltd.); vehicle and negative control: acetone/DMSO 9:1 (Vehicle).

[0257] 3. Experimental Procedures

[0258] 3.1 The animals were randomly divided into 6 groups based on body weight with 10 animals per group: normal control group, model control group, positive control group (dexamethasone sodium phosphate injection 5 mg/mL), 1% Compound 24 group, 0.3% Compound 24 group and 0.1% Compound 24 group.

[0259] 3.2 Modelling and administration: after adaptive feeding for several days, for each of the rats, hair in central area of the back (2 cm×2 cm) was shaved, and then the surface short hair was shaved with mild depilatory cream, washed and dried. If hair on the back grew out again during the experiment, the hair was removed with a razor. Every morning, for the rats in model control group and administration groups, 5% imiquimod cream was evenly applied on the back at 20 mg/cm.sup.2 (0.25 g/pack, for 3 rats), and after 10 min, recombinant human interferon α-2a injection was injected intraperitoneally at 10,000 U/500 g; for the normal control group, the rats were not treated, once a day, for 10 days. In the afternoon, for the rats in the administration groups, drugs were applied at a corresponding dose (drugs with corresponding concentrations were applied on the back at 100 μL/rat, twice, 50 μL each time) once a day for 10 days.

[0260] 3.3 Psoriasis Area and Severity Index (PASI) score: according to PASI scoring standard, a score of 0-4 was given for erythema, scale and epidermal thickening of infiltration in the lesional skin of rats. The scoring criteria was as follows: 0, none; 1, mild; 2, moderate; 3, severe; 4, extremely severe. For the rats in each of the groups, the scores were averaged and changes in skin lesions of the rats were observed.

[0261] 3.4 HE staining: after completion of modeling (on day 11), the tissues with skin lesions on the back of rats were collected, fixed with 4% neutral formaldehyde, embedded in paraffin, and sectioned. After HE staining, changes in skin histomorphology were observed under a microscope, and Baker scoring was performed. The specific criteria for Baker scoring were as follows: Munro's microabscess as found in the epidermis: a score of 2.0; hyperkeratosis: a score of 0.5; parakeratosis: a score of 1.0; thinning or disappearing of the granular layer: a score of 1.0; acanthosis: a score of 1.0; mild, moderate and severe lengthening and clubbing of rete ridges: scores of 0.5, 1.0 and 1.5, respectively; mild, moderate and severe infiltration of mononuclear or multinuclear cells in the dermis: scores of 0.5, 1.0 and 1.5, respectively; thinning above papillae: a score of 0.5; capillarectasis: a score of 0.5.

[0262] 3.5 Statistical Methods: all data was expressed as mean±standard deviation (x±s). SPSS 20.0 statistical software was used to process the data. One-way ANOVA was used to compare the mean values of multiple samples. P<0.05 was considered statistically significant. A rank sum test was used for the data that is not normally distributed or of unequal variance.

[0263] 4. Conclusion: all the results of PASI and Baker scores, and pathological analysis demonstrated that Compound 24 had a significant effect on psoriasis in a dose-dependent manner. The experimental results were shown in FIGS. 2 and 3.