SOLUTION FOR PRESERVING BIOLOGICAL SAMPLES AND USE OF SUCH SOLUTION

Abstract

A solution for preserving biological samples, includes methyl ethyl ketone peroxide with a percentage by volume between 5% and 25%. There is also a use of a solution for preserving biological samples including methyl ethyl ketone peroxide with a percentage by volume between 5% and 25%. The use includes immersing the sample in the solution.

Claims

1-10. (canceled)

11. A solution for preserving biological samples, comprising methyl ethyl ketone peroxide with a percentage by volume comprised between 5% and 25%.

12. The solution according to claim 11, further comprising dimethyl sulfoxide.

13. The solution according to claim 11, wherein said methyl ethyl ketone peroxide has a percentage by volume of 10%.

14. The solution according to claim 12, wherein said dimethyl sulfoxide has a percentage by volume of 90%.

15. The solution according to claim 11, further comprising one or more organic solvents.

16. The solution according to claim 11, further comprising water.

17. Use of a solution for preserving biological samples according to claim 11.

18. The use according to claim 17, wherein a biological sample is immersed in said solution.

19. The use according to claim 17, wherein said solution is applied superficially on a biological sample.

20. The use according to claim 17, wherein said solution is inoculated inside vascular and/or anatomical cavities of a biological sample.

Description

DETAILED DESCRIPTION OF THE DISCLOSURE

[0021] A solution for preserving biological samples according to the disclosure comprises methyl ethyl ketone peroxide with a percentage by volume comprised between 5% and 25%.

[0022] In this manner, the risks of explosion and corrosion are reduced with respect to conventional solutions.

[0023] In a preferred embodiment, the methyl ethyl ketone peroxide of the solution has a percentage by volume of 10%.

[0024] Such solution also comprises dimethyl sulfoxide.

[0025] In a preferred embodiment of the solution, the dimethyl sulfoxide has a percentage by volume of 90%.

[0026] Dimethyl sulfoxide has no contraindications, unlike methyl ethyl ketone peroxide, therefore the higher its percentage by volume and the lower that of methyl ethyl ketone peroxide, the lower the risks of explosion or corrosion.

[0027] Experimental tests and trials in the field have shown that a percentage by volume in a solution of methyl ethyl ketone peroxide comprised between 5% and 25% is sufficient to preserve biological samples.

[0028] The solution according to the present disclosure can comprise other elements in addition to methyl ethyl ketone peroxide and dimethyl sulfoxide, such as one or more organic solvents, such as for example glycerol and/or liquid petrolatum, and water.

[0029] In a preferred embodiment, the solution has a composition constituted solely by:

[0030] 10% by volume of methyl ethyl ketone peroxide,

[0031] 90% by volume of dimethyl sulfoxide.

[0032] The disclosure also relates to the use of a solution like the one described above for preserving biological samples.

[0033] In the present description, the term “preserving” means slowing down the processes of chemical/physical deterioration that begin as a consequence of the removal of biological samples or of the death of the organism from which the biological sample in question is removed.

[0034] In the present description, the expression “biological sample” means any organ, cell, tissue or even entire organism that needs to be preserved.

[0035] A first form of use of a solution, as described previously, entails the immersion of the biological sample inside such solution.

[0036] A second form of use of a solution, as described previously, entails a superficial application of such solution on the biological sample, for example by way of spreading.

[0037] A third form of use of a solution, as described previously, entails an inoculation of such solution inside vascular and/or anatomical cavities of the biological sample.

[0038] In practice it has been found that the disclosure fully achieves the intended aim and advantages by providing a solution for preserving biological samples that is not carcinogenic or toxic for the user and which is less explosive and less corrosive than the solutions known today.

[0039] The disclosure thus conceived is susceptible of numerous modifications and variations, all of which are within the scope of the appended claims. Moreover, all the details may be substituted by other, technically equivalent elements.

[0040] In practice the materials employed, provided they are compatible with the specific use, and the contingent dimensions and shapes, may be any according to requirements and to the state of the art.

[0041] The disclosures in Italian Patent Application No. 102019000000661 from which this application claims priority are incorporated herein by reference.