Efficient method for the preparation of Cangrelor

Abstract

Disclosed is a process for the preparation of Cangrelor in salt form by preparation and subsequent hydrolysis of an intermediate of formula (IV): ##STR00001## The process is characterized by the high yield and purity of the product, and can be used industrially.

Claims

1. A process for the preparation of cangrelor in the form of a salt of formula (V): ##STR00008## wherein M.sup.+is selected from sodium ion, ammonium ion and tributylammonium ion, said process comprising the following steps: a) phosphorylating a compound of formula (I) in the presence of phosphorus oxyhalide: ##STR00009## to give a compound of formula (II): ##STR00010## b) reacting the resulting compound of formula (II) with clodronic acid to give a compound of formula (IV): ##STR00011## c) optionally isolating the resulting compound of formula (IV) by precipitation from the reaction mixture and filtration, and d) hydrolysing the compound of formula (IV) to give cangrelor in the form of a salt of formula (V) by reaction with water in the presence of a base.

2. The process according to claim 1, wherein the compound of formula (V) is cangrelor tetrasodium salt.

3. The process according to claim 1, wherein the compound of formula (II) is not isolated.

4. The process according to claim 1, wherein the compound of formula (V) in the form of a salt obtained in step d) is purified by column chromatography and/or crystallisation from a suitable solvent.

5. The process according to claim 4, wherein the compound of formula (V) in the form of a salt obtained in step d) is purified by column chromatography and subsequent crystallisation from a suitable solvent.

6. The process according to claim 1, wherein the phosphorus oxyhalide is phosphoryl chloride.

7. The process according to claim 1, wherein the phosphorylation of step a) is carried out in an aprotic polar solvent selected from the group consisting of tetrahydrofuran, methyltetrahydrofuran, acetonitrile, dichloromethane, trimethyl phosphate and triethyl phosphate or a mixture thereof.

8. The process according to claim 1, wherein the phosphorylation of step a) is carried out at a temperature ranging from −20° C. to 40° C.

9. The process according to claim 1, wherein the reaction of step b) is carried out by adding the reaction mixture obtained in step a) to a clodronic acid solution in the presence of an organic base selected from the group consisting of triethylamine, tributyl amine, di-isopropyl ethyl amine, n-methyl morpholine, N,N-dimethylaniline and dicyclohexylamine.

10. The process according to claim 9, wherein clodronic acid is dissolved in a solvent selected from tetrahydrofuran, dioxane, methyl tert-butyl ether, ethyl acetate, cyclohexane, toluene, dichloromethane and acetonitrile.

11. The process according to claim 9, wherein the addition is carried out maintaining the two solutions at a temperature ranging from −20° C. to 40° C.

12. The process according to claim 1, wherein the precipitation of the compound of formula (IV) in step (c) is obtained by addition of an antisolvent.

13. The process according to claim 1, wherein the compound of formula (IV) obtained in step b) is not isolated.

14. The process according to claim 1, wherein the hydrolysis of step d) is carried out under basic hydrolysis conditions, in the presence of water and an organic solvent, or only in an aqueous medium.

15. The process according to claim 14, wherein the basic hydrolysis is carried out using an organic or inorganic base selected from the group consisting of ammonia, ammonium carbonate, ammonium bicarbonate, sodium carbonate, sodium bicarbonate, triethylamine, diisopropylamine and tributylamine.

16. The compound of formula (IV): ##STR00012##

17. The process according to claim 2, wherein the compound of formula (II) is not isolated.

18. The process according to claim 2, wherein the compound of formula (V) in the form of a salt obtained in step d) is purified by column chromatography and/or crystallisation from a suitable solvent.

19. The process according to claim 3, wherein the compound of formula (V) in the form of a salt obtained in step d) is purified by column chromatography and/or crystallisation from a suitable solvent.

20. The process according to claim 2, wherein the phosphorus oxyhalide is phosphoryl chloride.

Description

DETAILED DESCRIPTION OF THE INVENTION

(1) The present invention relates to a process for obtaining Cangrelor in the form of a pharmaceutically acceptable salt of formula (V):

(2) ##STR00004##

(3) wherein M.sup.+ is selected from sodium ion, ammonium ion and tributylammonium ion, said process comprising the following steps:

(4) a) phosphorylating a compound of formula (I) in the presence of a phosphorus oxyhalide:

(5) ##STR00005##

(6) to give a compound of formula (II):

(7) ##STR00006##

(8) reacting the resulting compound of formula (II) with clodronic acid to give the compound of formula (IV):

(9) ##STR00007##

(10) c) optionally isolating the resulting compound of formula (IV) by precipitation from the reaction mixture and filtration, and

(11) d) converting compound (IV) to Cangrelor in the form of a salt of formula (V) by reacting with water in the presence of a base.

(12) The compound of formula (V) is preferably Cangrelor tetrasodium salt.

(13) Preferably, compound of formula (II) is not isolated, and the reaction of compound of formula (II) with clodronic acid (step b) is effected directly on the reaction mixture obtained in step a).

(14) Cangrelor in salt form is preferably purified by column chromatography and/or crystallisation from a suitable solvent, more preferably by column chromatography and subsequent crystallisation from a suitable solvent.

(15) The phosphorylation of step a) is effected in the presence of a phosphorus oxyhalide such as phosphoryl chloride in a suitable polar aprotic solvent such as tetrahydrofuran, methyltetrahydrofuran, acetonitrile, dichloromethane, trimethyl phosphate and triethyl phosphate or a mixture thereof, preferably tetrahydrofuran, trimethyl phosphate and triethyl phosphate or a mixture thereof, specifically triethyl phosphate and trimethyl phosphate.

(16) The reaction is effected at a temperature ranging from −20° C. to 40° C., preferably from −20° C. to 20° C., for a reaction time ranging from 10 hours to 48 hours, specifically between 15 hours to 24 hours.

(17) The reaction of step b) is effected by adding the reaction mixture obtained in step a) to a clodronic acid solution of formula (III) in the presence of a suitable organic base such as triethyl amine, tributyl amine, di-isopropyl ethyl amine, n-methyl morpholine, N,N-dimethylaniline and dicyclohexyl amine, preferably triethylamine and tributylamine in a suitable solvent such as ethers, esters, alkanes and nitriles, specifically tetrahydrofuran, dioxane, methyl tert-butyl ether, ethyl acetate, cyclohexane, toluene dichloromethane and acetonitrile, preferably tetrahydrofuran, dichloromethane and acetonitrile. The addition is effected by maintaining the two solutions at a temperature ranging from −20° C. to 40° C., preferably from −20° C. to 20° C., and is maintained under stirring for a time ranging from 10 minutes and 180 minutes, preferably from 30 minutes and 120 minutes.

(18) The resulting compound of formula (IV) can be isolated by precipitation (step c) by adding an anti-solvent such as an apolar solvent belonging to the class of ethers or alkanes, such as ethyl ether, isopropyl ether, methyl tert-butyl ether, cyclopentyl methyl ether, n-hexane, cyclohexane and heptane. Compound (IV) is then recovered by filtration of the reaction mixture “as is” or after concentration to a suitable amount of solvent at a temperature ranging from 0° C. to 50° C., specifically between 0° C. to 30° C., to obtain the product as a crystalline solid.

(19) Alternatively, the isolation of compound of formula (IV) can be omitted and the reaction of step d) can be effected directly by adding a basic aqueous solution to the reaction medium to obtain Cangrelor in the form of a salt.

(20) Compound of formula (V) is prepared by reacting intermediate of formula (IV) under basic hydrolysis conditions, in the presence of water and an organic solvent such as ethers, alkanes and nitriles, specifically tetrahydrofuran, dichloromethane, toluene and acetonitrile, or in an aqueous medium only. The reaction is effected in the presence of an organic or inorganic base, specifically ammonia, ammonium carbonate, ammonium bicarbonate, sodium carbonate, sodium bicarbonate, triethylamine, diisopropylamine or tributylamine. The reaction is effected at a temperature ranging from 0° C. to 50° C., specifically between 0° C. to 30° C., and maintained under stirring for a time ranging from 2 hours to 72 hours, preferably from 16 hours to 36 hours.

(21) The product of formula (V) is isolated by chromatography on polymer resin such as HP20SS, eluting with a mixture of water and acetonitrile, followed by freeze-drying. The product thus isolated exhibits high purity.

(22) The product of formula (V) can be purified by crystallisation from a solvent such as acetone, methanol, ethanol or isopropanol or a mixture of one of said substances with water.

(23) The process according to the invention produces Cangrelor in the form of a salt with a high yield and purity. This efficient, novel process avoids the use of large molar amounts of reagents, and allows the synthetic intermediate (cyclic compound IV) to be isolated as a crystalline product. As a result of said improvements, the purification of Cangrelor is less difficult than with the known processes and can be effected with a common polymer resin, using a mixture of water and acetonitrile as eluent phase.

(24) The process of the invention has numerous advantages. They include isolation by crystallisation of cyclic compound (IV), which already has a good level of purity, thereby eliminating high-boiling solvents (which are difficult to remove), excess reagents and the associated by-products from the reaction medium. The end product can then be isolated by fast chromatography with a common polymer resin, by freeze-drying the enriched fractions eluted with water.

(25) Alternatively, the isolation of cyclic compound (IV) can be omitted, and a basic aqueous solution can be added directly to the reaction medium to obtain Cangrelor in the form of a salt which, once again, can be isolated by fast chromatography with a common polymer resin, by freeze-drying the enriched fractions eluted with water.

(26) In both cases the overall yield of the process is much higher than in the processes previously described, and amounts to about 70% molar.

(27) The compound of formula (I) and the reagents used in the process claimed are commercially available.

(28) A further object of the invention is the intermediate of formula (IV).

(29) The invention will now be further illustrated by the following examples.

EXAMPLES

Example 1

Synthesis of Compound of Formula (IV)

(30) Compound I (30.0 g, 0.06 mol) was dissolved in triethyl phosphate (150 mL), and the resulting mixture was cooled to 0° C. Phosphoryl chloride (18.5 g, 0.12 mol) was added in 30 minutes, and the reaction mixture was maintained at 0° C. for 24 hours. The reaction mixture was added in 60 minutes to a mixture of triethyl amine (77.5 g, 0.77 mol) and clodronic acid (27.6 g, 0.06 mol) in dichloromethane (450 mL). The reaction mixture was maintained under stirring at 0° C. for 60 minutes, cyclopentyl methyl ether (300 mL) was then added, and 150 ml of solvent was distilled at low pressure. The resulting suspension was filtered through a Buchner funnel to obtain the compound of formula (IV) as a whitish crystalline solid.

Example 2

Synthesis of Cangrelor Tetrasodium Salt

(31) The compound of formula (IV) obtained in example 1 (30.0 g, 0.02 mol) was suspended in dichloromethane (50 mL). A 10% aqueous solution of NaHCO.sub.3 (70 mL) was added to the suspension. The reaction mixture was maintained under stirring at 20° C. for 24 hours. 50 mL of solvent was distilled at low pressure. The resulting solution was loaded onto a chromatography column containing HP20SS resin. The column was eluted using a mixture of water and acetonitrile (95/5) as eluent. The product was isolated by concentrating the enriched fractions at low pressure, and finally crystallised from acetone to obtain Cangrelor as a white crystalline solid (4.8 g, 0.006 mol with over 99% purity (HPLC)).