MULTIPURPOSE BIOLOGICAL COMPOSITIONS

Abstract

Multipurpose biological compositions, for the biological control of phytopathogens, phytonematodes, agricultural pests, inoculating agent, phosphorus solubilization, soil conditioning, biostimulant, reduction of hydric stress in cultivated plants and bioremediation in cultivatable areas, contains mixtures of additives and excipients with at least two species from Bacilales order; or at least two strains from the same species, or mutants thereof; (b) at least two fungi from Hypocreales, Orbiliales, Helotiales and Mucorales order, and mutants thereof; or at least two strains from the same species from Hypocreales, Orbiliales, Helotiales and Mucorales orders; and combinations among the whole or part thereof; in c.f.u., in association with or separated from of agrochemicals, essential oil of plants, and plant extract.

Claims

1-19. (canceled)

20. A multipurpose biological composition comprising: (a) 1.0 to 90.0% w/v of at least two species from Bacilales order; or at least two strains from the same species, or mutants thereof; (b) 1.0 to 90.0% w/v of at least two fungi from Hypocreales, Orbiliales, Helotiales and Mucorales order, and mutants thereof; or at least two strains from the same species from Hypocreales, Orbiliales, Helotiales and Mucorales orders; and combinations among the whole or part thereof; in c.f.u., in association with or separated from, 5.0 to 10.0% of agrochemicals, 5.0 to 20.0% of essential oil of plants, 5.0 to 20.0% of plant extract; (c) 1.0 to 15.0% of an additive; and (d) 1.0 to 97.0% of an excipient.

21. The composition according to claim 20, wherein the Bacillus species from Bacilales order are selected from the group consisting of Bacillus velezensis, Bacillus aryabhattai, Bacillus genus, Bacillus pseudomycoides, Bacillus mucilaginosus, Bacillus sonorensis, Bacillus agri, Bacillus aizawai, Bacillus albolactis, Bacillus firmus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus kustaki, Bacillus lacticola, Bacillus lactimorbus, Bacillus lactis, Bacillus laterosporus, Bacillus lentimorbus, Bacillus megaterium, Bacillus medusa, Bacillus metiers, Bacillus natto, Bacillus nigrificans, Bacillus popilliae, pumilus, Bacillus siamensis, Bacillus subtilis, Bacilus licheniformis, Bacilus paralicheniformis, Bacillus amyloliquefasciens, Bacillus megaterium, Bacillus sphaericus, Bacillus thuringiensis; and combinations thereof.

22. The composition according to claim 20, wherein the fungi from Hypocreales order are selected from the group consisting of Trichoderma amazonicum, Trichoderma asperellum, Trichoderma atroviride, Trichoderma aureoviride, Trichoderma austrokoningii, Trichoderma brevicompactum, Trichoderma cinnamomem, Trichoderma hamatum, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma polysporum, Trichoderma pseudokoningii, Trichoderma reeset, Trichoderma stromaticum, Trichoderma vixens, Trichoderma viride, Trichoderma viridescens, Clonostachys agarwalii, Clonostachys asymmetrica, Clonostachys aurantia, Clonostachys chlorina, Clonostachys compacta, Clonostachys cylindrospora, Clonostachys farinosa, Clonostachys pseudobotrytis, Clonostachys pulvinata, Clonostachys rosea, (Link) Schroers, Clonostachys spectabilis, Clonostachys theobromae, Purpuriocillium lilacinus, Pochonia bulbillosa, Pochonia chlamydosporia, Pochonia chlamydosporia varo catenulata, Pochonia globispora, Pochonia goniodes, Pochonia humicola, Pochonia microbactrospora, Pochonia parasitica, Pochonia rubescens, Pochonia suchlasporia, Metarhizium anisopliae, Metarhizium acridum, Metarhizium brunneum, Metarhizium flavoviride, Metarizium rileyi, Aschersonia cubensi, Aschersonia aleyrodis, Aschersonia australiensis Aschersonia placenta, Isaria fumosorosea, Lecanicillium lecanni, Lecanicillium longisporum, Lecanicillium muscarium, Lecanicillium fungicola, Cordyceps militaris, Harposporium anguillulae; and combinations thereof.

23. The composition according to claim 20, wherein the fungi from Orbiliales order are selected from the group consisting of Arthrobotrys oligospora, Arthrobotrys aggregata, Arthrobotrys alaskana, Arthrobotrys amerospora, Arthrobotrys botryospora, Arthrobotrys brochopaga, Arthrobotrys conoides, Arthrobotrys constringens, Arthrobotrys cylindrospora, Arthrobotrys dactyloides, Arthrobotrys elegans, Arthrobotrys ellipsospora, Arthrobotrys entomopaga, Arthrobotrys globospora, Arthrobotrys irregulares, Arthrobotrys javanica, Arthrobotrys longispora, Arthrobotrys musiformis, Arthrobotrys nematopaga, Arthrobotrys oligospora, Dactylaria haptotyla, Dactylaria purpurella, Dactylaria scaphoides, Dactylaria brochopaga, Dactylaria candida; and combinations thereof

24. The composition according to claim 20, wherein the fungi from Helotiales order are selected from the group consisting of Monacrosporium Monacrosporium robustum, Monacrosporium appendiculatum, Monacrospium ellipsosporum, Monacrosporium eudermatum, Monacrospium fusiforme, Monacrosporium globosporum, Monacrosporium leptosporum, Monacrosporium cionopagu, Dactylella alba, Dactylella aphrobrocha, Dactylella arcuata, Dactylella arnaudii, Dactylella atractoides, Dactylella attenuata, Dactylella candida, Dactylella cionopaga, Dactylella fusiformis, Dactylella heterospora, Dactylella intermedia, Dactylella oxyspora, Dactylella ramose; and combinations thereof.

25. The composition according to claim 20, wherein the fungus from order Mucorales is Cunninghamella elegans.

26. The composition according to claim 20, wherein the agrochemicals are insecticides, fungicides, herbicides or nematocides.

27. The composition according to claim 20, wherein the essential oils and plant extract are obtained from seeds, plants and parts of plants, selected from the group consisting of Ricinus communis, Crotalaria juncea, Chenopodium ambrosioides, Aradirachia indica, Verbena officinalis, Erythrina mulungu, Quassia amara, Bidens pilosa, Plantago lanceolata, Senecio brasiliensis, Lantana camara, Tagetes patula, Lantana montevidensis, Eucalyptus spp., Lantana montevidensis, Lantana camara, Senecio brasiliensis, Chrysantemum coronarium, Solanum americanum, Solanum paniculatum Hovenia dulcis, Melia azedarach, Butia eriospatha, Schinus terenbithifolius, Cinnamomum camphora, Conyza bonariensis, Senecio brasiliensis, Bidens pilosa, Amaranthus hybridus, Euphorbia heterophylla, Raphanus sativus, Ipomoea purpurea, Brachiaria plantaginea, Ruta graveolens, Aloysio triphylla, Brassica napus, Mucuna pruriens, Ocimum gratissimum, Lippia sidoides, Croton zehntneri, Mentha piperita, Cymbopogon martinii, Cymbopogon schoenanthus, Piper aduncum, Arisaema franchetianum, Zanthoxylum simulans, Ruta chalepensis, Citrus sinensis, Melaleuca quinquenervia, Cymbopogon citratus, Thymus vulgaris, Piper aduncum, Melaleuca alternifolia, Hesperozygis myrtoides, and combinations thereof.

28. The composition according to claim 20, wherein the additives are dispersants selected from the group consisting of water-soluble ionic polymers; amino acids; algae extract; naturally occurring compounds; water-soluble anionic polymers; surfactants selected from the group consisting of anionic surfactants and non-ionic surfactants; and derivatives thereof; and combinations thereof.

29. The composition according to claim 20, wherein the excipients are selected from the group consisting of silicas; talc; bentonite; carbohydrates; sugars; starches; carbonates; milk derivatives selected from the group consisting of whey and powder milk; and derivatives thereof; and combinations thereof.

30. The composition according to claim 20, formulated as an encapsulated formulation suspension, dispersible concentrate, emulsifiable concentrate, water-in-oil emulsion, oil-in-water emulsion, microemulsion, concentrated suspension, suspo-emulsion, soluble granulate, soluble concentrate, soluble powder, tablet, dispersible granulate, wettable powder, emulsifiable gel, water-soluble gel, emulsifiable granule, emulsifiable powder, oil dispersion or oil concentrated suspension, mixed formulation of soluble concentrate and concentrated suspension, mixed formulation of soluble concentrate and suspo-emulsion, mixed formulation of soluble concentrate and oil-in-water emulsion, oil-concentrated dispersible or miscible suspension, oil-miscible solution, oil-dispersible powder, encapsulated granulate, dry powder, liquid for electrostatic/electrodynamic spray, granulate, spray/spread oil, ultra-low volume suspension, ultra-low volume micro granulate, thin powder, thin granulate, macrogranulate, encapsulation, microencapsulation and nanoencapsulation.

31. A method for spraying, bar spraying, aerial spraying, low volume application, ultra-low volume application, drench spraying, drenching of cultivated plants; comprising administering the composition according to claim 20 during spraying, bar spraying, aerial spraying, low volume application, ultra-low volume application, drench spraying and drenching of cultivated plants.

32. A method for coating, treating, and encrusting of industrial or non-industrial seeds comprising administering the composition according to claim 20 for coating, treating, and encrusting of industrial or non-industrial seeds.

33. A method for controlling phytopathogens in cultivated plants, comprising administering the composition according to claim 20 for controlling phytopathogens.

34. A method for controlling phytonematodes in cultivated plants, comprising administering the composition according to claim 20 for controlling phytonematodes.

35. A method for controlling agricultural pests in cultivated plants, comprising administering the composition according to claim 20 for controlling agricultural pests.

36. A method for enhancing the growth of cultivated plants, comprising administrating the composition of claim 20 as a biostimulant, as an inoculant, as a phosphorus solubilizer, and as a growth stimulant for enhancing the growth of cultivated plants.

37. A method for conditioning a soil for the growth of cultivated plants, comprising administering the composition according to claim 20 for the growth of cultivated plants.

38. A method for conditioning a soil for mitigating or reducing the hydric or abiotic stress in cultivated plants, comprising administering the composition according to claim 20 for mitigating or reducing the hydric or abiotic stress in cultivated plants.

39. A method for bioremediating cultivable areas for reducing the contamination of the environment by toxic waste and non-toxic waste, comprising administering the composition according to claim 20 for bioremediating cultivable areas for reducing the contamination of the environment by toxic waste and non-toxic waste.

Description

EXAMPLE 1

[0032] Bacillus amyloliquefasciens (CPQBA 040-11 DRM 01 strain)—1.0×10.sup.9 CFU/g
Bacillus amyloliquefasciens (CPQBA 040-11 DRM 04 strain)—1.0×10.sup.9 CFU/g

EXAMPLE 2

[0033] Bacillus amyloliquefasciens: 1.0×10.sup.9 CFU/g
Bacillus liqueniformis: 1.0×10.sup.9 CFU/g

EXAMPLE 3

[0034] Bacillus subtilis: 1.0×10.sup.9 CFU/g
Bacillus amyloliquefaciens: 1.0×10.sup.9 CFU/g
Trichoderma harzianum: 1.0×10.sup.7 CFU/g

EXAMPLE 4

[0035] Beauveria bassiana: 1.0×10.sup.9 CFU/g
Bacillus thuringiensis: 1.0×10.sup.9 CFU/g
Aschersonia aleyrodis: 1.0×10.sup.7 CFU/g

EXAMPLE 5

[0036] Beauveria bassiana: 1.0×10.sup.6 CFU/g
Isaria fumosorosea: 1.0×10.sup.6 CFU/g
Aschersonia aleyrodis: 1.0×10.sup.6 CFU/g

EXAMPLE 6

[0037] Beauveria bassiana: 1.0×10.sup.6 CFU/g
Aschersonia aleyrodis: 1.0×10.sup.6 CFU/g

[0038] By way of example, the composition can have the following concentrations:

EXAMPLE 1

[0039] Bacillus amyloliquefasciens (CPQBA 040-11 DRM 01 strain): 1.0 to 20.0%
Bacillus amyloliquefasciens (CPQBA 040-11 DRM 04 strain): 1.0 to 20.0%

Additives: 1.0 to 20.0%

Excipients: 97.0 to 40.0%

EXAMPLE 2

[0040] Bacillus amyloliquefasciens: 1.0 to 20.0%
Bacillus liqueniformis: 1.0 to 20.0%
Melaleuca quinquenervia oil: 10.0 to 15.0%

Additives: 1.0 to 15.0%

Excipients: 86.0 to 30.0%

EXAMPLE 3

[0041] Bacillus subtilis: 1.0 to 20.0%
Bacillus amyloliquefaciens: 1.0 to 20.0%
Trichoderma harzianum: 1.0 to 20.0%

Azoxistrobin: 5.0 to 10.0%

Additives: 1.0 to 20.0%

Excipients: 92.0 to 10.0%

EXAMPLE 4:

[0042] Beauveria bassiana: 10.0 to 20.0%
Bacillus thuringiensis: 10.0 to 20.0%
Aschersonia aleyrodis: 10.0 to 20.0%

Additives: 1.0 to 20.0%

Excipients: 69.0 to 20.0%

EXAMPLE 5:

[0043] Beauveria bassiana: 10.0 to 20.0%
Isaria fumosorosea: 10.0 to 20.0%
Aschersonia aleyrodis: 10.0 to 20.0%

Additives: 1.0 to 20.0%

Excipients: 69.0 to 20.0%

EXAMPLE 6:

[0044] Beauveria bassiana: 10.0 to 20.0%
Aschersonia aleyrodis: 10.0 to 20.0%

Additives: 1.0 to 20.0%

Excipients: 69.0 to 40.0%.

EXAMPLES OF MODES TO OBTAIN THE COMPOSITION

[0045] A composition containing 10.0% Bacillus amyloliquefasciens (CPQBA 040-11 DRM 01 strain), 10.0% Bacillus amyloliquefasciens (CPQBA 040-11 DRM 04 strain), 3% acrylic styrene polymer, 2.0% anionic surfactant and 75.0% inert material was formulated in order to evaluate its efficiency in the control of phytopathogens, biostimulation, growth promotion and soil conditioning.

[0046] A composition containing 10.0% Bacillus subtilis, 10.0% Bacillus amyloliquefasciens, 10.0% Malaleuca quinquenervia oil, 6.0% anionic surfactant and 64.0% inert material was formulated in order to evaluate its efficiency in the control of phytopathogens.

[0047] A composition containing 10.0% Bacillus amyloliquefasciens, 8.0% Bacillus licheniformis, 10% Trichoderma harzianum, 18.0% Araxistrobin, 7.5% anionic surfactant and 46.5% inert material was formulated in order to evaluate its efficiency in the control of phytopathogens, control of nematodes, growth promotion and soil conditioning.

[0048] A composition containing 15.0% Beauveria bassiana, 7.0% Bacillus thuringiensis, 20.0% Aschersonia aleyrodis, 4.0% acrylic styrene polymer, 2.5% anionic surfactant and 51.5% inert material was formulated in order to evaluate its efficiency in the control of insects.

[0049] A composition containing 15.0% Beauveria bassiana, 15.0% Isaria fumosorosea, 20.0% Aschersonia aleyrodis, 4.0% acrylic styrene polymer, 2.5% anionic surfactant and 43.5% inert material was formulated in order to evaluate its efficiency in the control of insects.

[0050] A composition containing 15.0% Beauveria bassiana, 20.0% Aschersonia aleyrodis, 4.0% acrylic styrene polymer, 2.5% anionic surfactant and 58.5% inert material was formulated in order to evaluate its efficiency in the control of insects.

[0051] The compositions for the biological control of phytopathogens, phytonematodes and insects, inoculants, soil conditioners, bioestimulants, hydric stress reducers in plants and bioremediation shall follow the flow of events below:

Raw material: the raw material that will make out the product shall be received and handled by skilled people;
Mixture: the raw material shall be weighed and mixed following the standard operational procedure (POP) at the indicated proportions;
Sampling: after the mixture, samples shall be taken in order to check and certify the product guarantee. The number of colony forming units (CFU/g or CFU/ml) of product;
Packing: the formulated product complying with the guarantee specifications shall be packed in previously labeled 1.0; 5.0 and 10.0 kg/l plastic bottles. The bottles/pouches are sealed and closed;
Storage: The bottles/pouches are accommodated on pallets and stored in a dry, aired light-protected place, and maintained in this condition until being dispatched.

[0052] The present invention can be applied through spraying, bar spraying, aerial spraying, low volume, ultra-low volume, drench spraying, drench, coating (either industrially or not), treatment (either industrially or not) and incrustation (either industrially or not) of crop seeds for the biological control of phytopathogens, phytonematodes, insects and mites, inoculants, soil conditioners, bioestimulants and hydric stress reducers for cultivated plants and bioremediation. The application method shall be analyzed case after case and shall depend on the technical conditions and needs of every producer.

Advantages Attained

[0053] The use of the composition for the biological control of phytopathogens, phytonematodes, insects and mites, inoculants, soil conditioners, bioestimulants and hydric stress reducers for cultivated plants and bioremediation containing mixtures of at least 2 (two) species from Bacillus, or 2 (two) or more strains of the same species and mutants thereof; at least 2 (two) representatives of fungi from orders Hypocreales, Orbiliales, Helotiales and Mucorales and mutants thereof, or (two) or more strains of the same species from representatives from orders of Bacillus mixed with fungi from orders Hypocreales, Orbiliales, Helotiales and Mucorales, and combinations among same or part of same (in colony forming units, CFU) together with or separated from agrochemicals (fungicides, insecticides, acaricides, herbicides and nematicides), essential oils of plants or plant extract, provides the following advantages:

It benefits the development of cultivated plants, providing increments in radicular growth, foliar and production parameters;
It is an alternative to the use of fungicides, nematicides, insecticides and acaricides, thus fulfilling the interest of the society concerning more environmentally friendly products;
It explores a wider range of mechanisms of action against phytonematodes, thus assuring higher efficiency;
It explores a wider range of mechanisms of action against phytopathogens, thus assuring higher efficiency;
It explores a wider range of mechanisms of action against insects and mites, thus assuring higher efficiency;
It explores a wider range of mechanisms of action in the fixation of nitrogen into plants, thus assuring higher nutritional efficiency;
It explores a wider range of mechanisms in soil conditioning, reducing phytopathogens and increasing the interaction with the roots and promoting the radicular and foliar growth of cultivated plants;
It explores a wider range of mechanisms of action against abiotic stresses, mainly the hydric stress, thus assuring higher efficiency in the retention of water and productive increment;
It explores different mechanisms of action in the degradation of substances deleterious to the environment, for instance, pesticides;
It reduces the selection of phytonematodes, phytopathogens and chemical-resistant insects;
It can be easily operated when used in spraying, drench spraying, drench and coating, treatment and incrustation of seeds, either industrially or not;
It can be used in constituents of coating, treatment and incrustation of seeds (either industrially or not) of Coffea spp (Coffea arabica, Coffea canefora, Coffea robusta), Glycine max, Zea mays, Gossypium hirsutum, greens (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), forest trees (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.), Nicotiana tabacum, ornamental plants, fruit trees (Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus persica, Passiflora edulis), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, Crotalaria (Crotalaria juncea, Crotalaria spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora) and other species from the above cited genera.

[0054] The scope of the present invention should not be limited to the practical examples, but to the terms defined in the claims and equivalents thereof.

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